TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2823202.2.1
(529 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|AJ311603.1|HVU311603 Hordeum vulgare mRNA for putative ... 142 1e-033
gb|BM816691.1|BM816691 HB01E06_T3.ab1 HB Hordeum vulgare su... 139 1e-032
gb|AJ434759.1|AJ434759 AJ434759 S00002 Hordeum vulgare subs... 139 1e-032
dbj|D83178.1| Hordeum vulgare mRNA for endonuclease, comple... 138 3e-032
gb|BM372230.2|BM372230 EBro03_SQ004_G04_R root, 3 week, wat... 136 1e-031
gb|BM372422.2|BM372422 EBro03_SQ004_O15_R root, 3 week, wat... 132 1e-030
gb|BF257877.2|BF257877 HVSMEf0014B21f Hordeum vulgare seedl... 129 9e-030
gb|AV920399.1|AV920399 AV920399 K. Sato unpublished cDNA li... 123 6e-028
gb|CB876328.1|CB876328 HX10P08w HX Hordeum vulgare subsp. v... 123 6e-028
gb|BQ665439.1|BQ665439 HX03J22u HX Hordeum vulgare subsp. v... 119 1e-026
gb|AV929820.1|AV929820 AV929820 K. Sato unpublished cDNA li... 111 3e-024
gb|BJ450513.1|BJ450513 BJ450513 K. Sato unpublished cDNA li... 111 3e-024
gb|BJ454144.1|BJ454144 BJ454144 K. Sato unpublished cDNA li... 111 3e-024
gb|BJ457937.1|BJ457937 BJ457937 K. Sato unpublished cDNA li... 111 3e-024
gb|BJ461676.1|BJ461676 BJ461676 K. Sato unpublished cDNA li... 111 3e-024
dbj|AB028448.1| Hordeum vulgare Bnuc1 mRNA for nuclease I, ... 111 3e-024
>emb|AJ311603.1|HVU311603 Hordeum vulgare mRNA for putative nuclease (bnuc2 gene)
Length = 1139
Score = 142 bits (305), Expect = 1e-033
Identities = 62/88 (70%), Positives = 68/88 (77%)
Frame = +3 / +2
Query: 6 RAIQQNITXXXXXXXXXXXXCRSRTKTCADKYAAESAKLACTAYEGVDQDSTLEDDYFFA 185
+AIQ+NIT CRSRTKTCADKYA ESA LAC AY+GV QDSTL D+Y+F
Sbjct: 647 KAIQRNITEDWSSEEKQWEACRSRTKTCADKYAEESAVLACDAYKGVKQDSTLGDEYYFK 826
Query: 186 ALPVVQKRIAQGGVRLAAILNRIFGGNS 269
ALPVVQKRIAQGGVRLAAILNRIF G +
Sbjct: 827 ALPVVQKRIAQGGVRLAAILNRIFSGKN 910
>gb|BM816691.1|BM816691 HB01E06_T3.ab1 HB Hordeum vulgare subsp. vulgare cDNA clone
HB01E06_T3.ab1 similar to ESTs
D48949(S15541),AU097625(S15541) correspond to a region
of the predicted gene. Similar to Arabidopsis thaliana
chromosome I BAC T22E19; putative bifunctional nuclease
(AC016447) [Oryza sativa], mRNA sequence
Length = 837
Score = 139 bits (298), Expect = 1e-032
Identities = 59/88 (67%), Positives = 67/88 (76%)
Frame = +3 / +3
Query: 9 AIQQNITXXXXXXXXXXXXCRSRTKTCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAA 188
+IQ+NIT CRS+T TCA+KYA ESA LAC AYEGV+QD TL D+Y+F A
Sbjct: 348 SIQRNITDDWSSEEKQWETCRSKTTTCAEKYAQESAVLACDAYEGVEQDDTLGDEYYFKA 527
Query: 189 LPVVQKRIAQGGVRLAAILNRIFGGNSR 272
LPVVQKR+AQGGVRLAAILNRIF GN R
Sbjct: 528 LPVVQKRLAQGGVRLAAILNRIFSGNGR 611
>gb|AJ434759.1|AJ434759 AJ434759 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200006A08F1, mRNA sequence
Length = 502
Score = 139 bits (298), Expect = 1e-032
Identities = 59/88 (67%), Positives = 67/88 (76%)
Frame = +3 / +3
Query: 9 AIQQNITXXXXXXXXXXXXCRSRTKTCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAA 188
+IQ+NIT CRS+T TCA+KYA ESA LAC AYEGV+QD TL D+Y+F A
Sbjct: 54 SIQRNITDDWSSEEKQWEACRSKTTTCAEKYAQESAVLACDAYEGVEQDDTLGDEYYFKA 233
Query: 189 LPVVQKRIAQGGVRLAAILNRIFGGNSR 272
LPVVQKR+AQGGVRLAAILNRIF GN R
Sbjct: 234 LPVVQKRLAQGGVRLAAILNRIFSGNGR 317
>dbj|D83178.1| Hordeum vulgare mRNA for endonuclease, complete cds
Length = 1180
Score = 138 bits (295), Expect = 3e-032
Identities = 58/88 (65%), Positives = 67/88 (76%)
Frame = +3 / +1
Query: 9 AIQQNITXXXXXXXXXXXXCRSRTKTCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAA 188
+IQ+NIT CRS+T TCA+KYA ESA LAC AYEGV+QD TL D+Y+F A
Sbjct: 643 SIQRNITDDWSSEEKQWETCRSKTTTCAEKYAQESAVLACDAYEGVEQDDTLGDEYYFKA 822
Query: 189 LPVVQKRIAQGGVRLAAILNRIFGGNSR 272
LPVVQKR+AQGG+RLAAILNRIF GN R
Sbjct: 823 LPVVQKRLAQGGLRLAAILNRIFSGNGR 906
>gb|BM372230.2|BM372230 EBro03_SQ004_G04_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ004_G04 5', mRNA sequence
Length = 526
Score = 136 bits (291), Expect = 1e-031
Identities = 58/88 (65%), Positives = 66/88 (75%)
Frame = +3 / +2
Query: 9 AIQQNITXXXXXXXXXXXXCRSRTKTCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAA 188
+IQ+NIT CRS+T TCA+KYA ESA LAC AYEGV+QD TL D+Y+F A
Sbjct: 14 SIQRNITDDWSSEEKQWETCRSKTTTCAEKYAQESAVLACDAYEGVEQDDTLGDEYYFKA 193
Query: 189 LPVVQKRIAQGGVRLAAILNRIFGGNSR 272
LPVVQKR+A GGVRLAAILNRIF GN R
Sbjct: 194 LPVVQKRLAXGGVRLAAILNRIFXGNGR 277
>gb|BM372422.2|BM372422 EBro03_SQ004_O15_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ004_O15 5', mRNA sequence
Length = 565
Score = 132 bits (283), Expect = 1e-030
Identities = 57/88 (64%), Positives = 64/88 (72%)
Frame = +3 / +1
Query: 9 AIQQNITXXXXXXXXXXXXCRSRTKTCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAA 188
+IQ+NIT CRS+T TCA+KYA ESA LAC AYEGV+QD TL D+Y+F A
Sbjct: 109 SIQRNITDDWSSEEKQWETCRSKTTTCAEKYAQESAVLACDAYEGVEQDDTLGDEYYFKA 288
Query: 189 LPVVQKRIAQGGVRLAAILNRIFGGNSR 272
LPVVQKR GGVRLAAILNRIF GN R
Sbjct: 289 LPVVQKRARPGGVRLAAILNRIFXGNGR 372
>gb|BF257877.2|BF257877 HVSMEf0014B21f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0014B21f, mRNA sequence
Length = 572
Score = 129 bits (277), Expect = 9e-030
Identities = 57/88 (64%), Positives = 64/88 (72%)
Frame = +3 / +2
Query: 9 AIQQNITXXXXXXXXXXXXCRSRTKTCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAA 188
+IQ+NIT CR T TCA+KYA ESA LAC AYEGV+QD TL D+Y+F A
Sbjct: 284 SIQRNITDDWSSEEKQWEACRIITTTCAEKYAQESAVLACDAYEGVEQDDTLGDEYYFKA 463
Query: 189 LPVVQKRIAQGGVRLAAILNRIFGGNSR 272
LPVVQ R+AQGGVRLAAILNRIF GN R
Sbjct: 464 LPVVQMRLAQGGVRLAAILNRIFIGNGR 547
>gb|AV920399.1|AV920399 AV920399 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags13m18 3', mRNA sequence
Length = 663
Score = 123 bits (264), Expect = 6e-028
Identities = 54/87 (62%), Positives = 61/87 (70%)
Frame = +3 / -2
Query: 9 AIQQNITXXXXXXXXXXXXCRSRTKTCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAA 188
AI+ N+T C + T TCADKYA ESA+L+C AY G D S LED+YFF A
Sbjct: 560 AIKHNLTNEWCREENQWAACYTPTTTCADKYAEESAELSCPAYVGADHGSNLEDEYFFKA 381
Query: 189 LPVVQKRIAQGGVRLAAILNRIFGGNS 269
LPVVQKRIAQGGVRLAAILNRIF G +
Sbjct: 380 LPVVQKRIAQGGVRLAAILNRIFSGKN 300
>gb|CB876328.1|CB876328 HX10P08w HX Hordeum vulgare subsp. vulgare cDNA clone HX10P08
3-PRIME, mRNA sequence
Length = 597
Score = 123 bits (264), Expect = 6e-028
Identities = 54/87 (62%), Positives = 61/87 (70%)
Frame = +3 / -1
Query: 9 AIQQNITXXXXXXXXXXXXCRSRTKTCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAA 188
AI+ N+T C + T TCADKYA ESA+L+C AY G D S LED+YFF A
Sbjct: 501 AIKHNLTNEWCREENQWAACYTPTTTCADKYAEESAELSCPAYVGADHGSNLEDEYFFKA 322
Query: 189 LPVVQKRIAQGGVRLAAILNRIFGGNS 269
LPVVQKRIAQGGVRLAAILNRIF G +
Sbjct: 321 LPVVQKRIAQGGVRLAAILNRIFSGKN 241
>gb|BQ665439.1|BQ665439 HX03J22u HX Hordeum vulgare subsp. vulgare cDNA clone HX03J22
3-PRIME, mRNA sequence
Length = 428
Score = 119 bits (255), Expect = 1e-026
Identities = 50/68 (73%), Positives = 55/68 (80%)
Frame = +3 / -3
Query: 66 CRSRTKTCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAALPVVQKRIAQGGVRLAAIL 245
C + T TCADKYA ESA+L+C AY G D S LED+YFF ALPVVQKRIAQGGVRLAAIL
Sbjct: 426 CYTPTTTCADKYAEESAELSCPAYVGADHGSNLEDEYFFKALPVVQKRIAQGGVRLAAIL 247
Query: 246 NRIFGGNS 269
NRIF G +
Sbjct: 246 NRIFSGKN 223
>gb|AV929820.1|AV929820 AV929820 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd16l09 3', mRNA sequence
Length = 688
Score = 111 bits (237), Expect = 3e-024
Identities = 46/62 (74%), Positives = 52/62 (83%)
Frame = +3 / -1
Query: 84 TCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAALPVVQKRIAQGGVRLAAILNRIFGG 263
TCA+KYA ESA LAC AYEGV+Q TL DDY+F ALPVV+KRIAQGGVRLA ILN+IF G
Sbjct: 472 TCAEKYAKESALLACDAYEGVEQGDTLGDDYYFRALPVVEKRIAQGGVRLAVILNQIFSG 293
Query: 264 NS 269
+
Sbjct: 292 KN 287
>gb|BJ450513.1|BJ450513 BJ450513 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak29p24 5', mRNA sequence
Length = 612
Score = 111 bits (237), Expect = 3e-024
Identities = 46/62 (74%), Positives = 52/62 (83%)
Frame = +3 / +1
Query: 84 TCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAALPVVQKRIAQGGVRLAAILNRIFGG 263
TCA+KYA ESA LAC AYEGV+Q TL DDY+F ALPVV+KRIAQGGVRLA ILN+IF G
Sbjct: 175 TCAEKYAKESALLACDAYEGVEQGDTLGDDYYFRALPVVEKRIAQGGVRLAVILNQIFSG 354
Query: 264 NS 269
+
Sbjct: 355 KN 360
>gb|BJ454144.1|BJ454144 BJ454144 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak46f01 5', mRNA sequence
Length = 621
Score = 111 bits (237), Expect = 3e-024
Identities = 46/62 (74%), Positives = 52/62 (83%)
Frame = +3 / +2
Query: 84 TCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAALPVVQKRIAQGGVRLAAILNRIFGG 263
TCA+KYA ESA LAC AYEGV+Q TL DDY+F ALPVV+KRIAQGGVRLA ILN+IF G
Sbjct: 188 TCAEKYAKESALLACDAYEGVEQGDTLGDDYYFRALPVVEKRIAQGGVRLAVILNQIFSG 367
Query: 264 NS 269
+
Sbjct: 368 KN 373
>gb|BJ457937.1|BJ457937 BJ457937 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak29p24 3', mRNA sequence
Length = 620
Score = 111 bits (237), Expect = 3e-024
Identities = 46/62 (74%), Positives = 52/62 (83%)
Frame = +3 / -2
Query: 84 TCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAALPVVQKRIAQGGVRLAAILNRIFGG 263
TCA+KYA ESA LAC AYEGV+Q TL DDY+F ALPVV+KRIAQGGVRLA ILN+IF G
Sbjct: 442 TCAEKYAKESALLACDAYEGVEQGDTLGDDYYFRALPVVEKRIAQGGVRLAVILNQIFSG 263
Query: 264 NS 269
+
Sbjct: 262 KN 257
>gb|BJ461676.1|BJ461676 BJ461676 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak46f01 3', mRNA sequence
Length = 617
Score = 111 bits (237), Expect = 3e-024
Identities = 46/62 (74%), Positives = 52/62 (83%)
Frame = +3 / -2
Query: 84 TCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAALPVVQKRIAQGGVRLAAILNRIFGG 263
TCA+KYA ESA LAC AYEGV+Q TL DDY+F ALPVV+KRIAQGGVRLA ILN+IF G
Sbjct: 430 TCAEKYAKESALLACDAYEGVEQGDTLGDDYYFRALPVVEKRIAQGGVRLAVILNQIFSG 251
Query: 264 NS 269
+
Sbjct: 250 KN 245
>dbj|AB028448.1| Hordeum vulgare Bnuc1 mRNA for nuclease I, complete cds
Length = 1283
Score = 111 bits (237), Expect = 3e-024
Identities = 46/62 (74%), Positives = 52/62 (83%)
Frame = +3 / +2
Query: 84 TCADKYAAESAKLACTAYEGVDQDSTLEDDYFFAALPVVQKRIAQGGVRLAAILNRIFGG 263
TCA+KYA ESA LAC AYEGV+Q TL DDY+F ALPVV+KRIAQGGVRLA ILN+IF G
Sbjct: 749 TCAEKYAKESALLACDAYEGVEQGDTLGDDYYFRALPVVEKRIAQGGVRLAVILNQIFSG 928
Query: 264 NS 269
+
Sbjct: 929 KN 934
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 257,741,931
Number of Sequences: 312970
Number of extensions: 4544468
Number of successful extensions: 173027
Number of sequences better than 1.0e-020: 21
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5341209875
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)