TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2762970.2.1
(578 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U46003.1|HVU46003 Hordeum vulgare beta-D-glucan exohydro... 220 7e-086
gb|AV925468.1|AV925468 AV925468 K. Sato unpublished cDNA li... 220 7e-078
gb|AL504655.1|AL504655 AL504655 Hordeum vulgare Barke roots... 220 7e-070
gb|BF257182.2|BF257182 HVSMEf0012B20f Hordeum vulgare seedl... 219 3e-068
gb|AV914506.1|AV914506 AV914506 K. Sato unpublished cDNA li... 133 6e-068
gb|BU977331.1|BU977331 HA11D20r HA Hordeum vulgare subsp. v... 220 7e-062
gb|CB883402.1|CB883402 HQ02C08w HQ Hordeum vulgare subsp. v... 219 2e-056
gb|AF102868.1| Hordeum vulgare subsp. vulgare beta-D-glucan... 129 3e-055
gb|BE411093.1|BE411093 ISC002.C07F990406 ITEC ISC Barley Le... 213 2e-054
gb|BU996833.1|BU996833 HM14O21r HM Hordeum vulgare subsp. v... 85 4e-052
gb|AV919570.1|AV919570 AV919570 K. Sato unpublished cDNA li... 119 2e-051
gb|BI947544.1|BI947544 HVSMEl0005N11f Hordeum vulgare spike... 128 1e-050
gb|BQ469930.1|BQ469930 HX01E12T HX Hordeum vulgare subsp. v... 128 4e-049
gb|AV913828.1|AV913828 AV913828 K. Sato unpublished cDNA li... 193 2e-048
gb|BG416639.1|BG416639 HVSMEk0013O04f Hordeum vulgare testa... 106 2e-048
gb|BM098044.2|BM098044 EBpi03_SQ002_G06_R pistil, 4 DPA, no... 96 7e-048
gb|CA032659.1|CA032659 HX13N01r HX Hordeum vulgare subsp. v... 189 2e-047
gb|AL503553.1|AL503553 AL503553 Hordeum vulgare Barke roots... 152 3e-044
gb|AV913362.1|AV913362 AV913362 K. Sato unpublished cDNA li... 119 1e-043
gb|AV918661.1|AV918661 AV918661 K. Sato unpublished cDNA li... 119 6e-043
gb|AV920100.1|AV920100 AV920100 K. Sato unpublished cDNA li... 119 6e-037
gb|AV930613.1|AV930613 AV930613 K. Sato unpublished cDNA li... 119 6e-037
gb|BQ765660.1|BQ765660 EBro03_SQ007_F19_R root, 3 week, wat... 119 6e-037
gb|AV911128.1|AV911128 AV911128 K. Sato unpublished cDNA li... 117 2e-036
gb|BF627275.3|BF627275 HVSMEb0004G19f Hordeum vulgare seedl... 119 4e-036
gb|AV909243.1|AV909243 AV909243 K. Sato unpublished cDNA li... 117 3e-035
gb|BQ761635.1|BQ761635 EBem10_SQ001_J09_R embryo, 2 Day ger... 116 4e-033
gb|BI951410.1|BI951410 HVSMEl0025N13f Hordeum vulgare spike... 120 2e-031
gb|AV917377.1|AV917377 AV917377 K. Sato unpublished cDNA li... 135 5e-031
gb|BU997464.1|BU997464 HI08B05r HI Hordeum vulgare subsp. v... 119 2e-030
gb|BU995937.1|BU995937 HM11N11r HM Hordeum vulgare subsp. v... 103 4e-030
gb|CA022582.1|CA022582 HZ43L07r HZ Hordeum vulgare subsp. v... 129 2e-029
gb|BM100567.2|BM100567 EBma01_SQ005_J17_R maternal, 4 DPA, ... 129 2e-029
gb|CA015249.1|CA015249 HT13L19r HT Hordeum vulgare subsp. v... 88 3e-029
gb|BQ468423.1|BQ468423 HM01D10T HM Hordeum vulgare subsp. v... 109 2e-028
gb|CB881387.1|CB881387 HM09K13w HM Hordeum vulgare subsp. v... 106 8e-028
gb|BQ663930.1|BQ663930 HU04K15u HU Hordeum vulgare subsp. v... 119 1e-027
gb|BQ464740.1|BQ464740 HU01E02T HU Hordeum vulgare subsp. v... 120 1e-026
gb|CA020043.1|CA020043 HV14C18r HV Hordeum vulgare subsp. v... 120 1e-026
gb|BJ467252.1|BJ467252 BJ467252 K. Sato unpublished cDNA li... 119 3e-026
gb|CB858339.1|CB858339 HI06K15w HI Hordeum vulgare subsp. v... 119 3e-026
gb|BM374002.2|BM374002 EBma03_SQ003_F17_R maternal, 8 DPA, ... 118 4e-026
gb|BM098018.2|BM098018 EBpi03_SQ002_F03_R pistil, 4 DPA, no... 118 4e-026
gb|BQ462564.1|BQ462564 HI01E18T HI Hordeum vulgare subsp. v... 117 1e-025
gb|BQ462668.1|BQ462668 HI01K09T HI Hordeum vulgare subsp. v... 117 1e-025
gb|BQ468807.1|BQ468807 HM02G11r HM Hordeum vulgare subsp. v... 106 2e-025
gb|BG366042.2|BG366042 HVSMEi0004P02f Hordeum vulgare 20 DA... 114 6e-025
gb|CA026644.1|CA026644 HZ56I06r HZ Hordeum vulgare subsp. v... 108 3e-024
gb|CA028903.1|CA028903 HZ63J10r HZ Hordeum vulgare subsp. v... 88 4e-024
gb|AV833400.1|AV833400 AV833400 K. Sato unpublished cDNA li... 87 8e-024
gb|CB882092.1|CB882092 HM11N11w HM Hordeum vulgare subsp. v... 103 1e-023
gb|BU996476.1|BU996476 HM13L18r HM Hordeum vulgare subsp. v... 102 3e-023
>gb|U46003.1|HVU46003 Hordeum vulgare beta-D-glucan exohydrolase, isoenzyme ExoII, mRNA,
complete cds
Length = 2105
Score = 220 bits (477), Expect(2) = 7e-086
Identities = 91/110 (82%), Positives = 98/110 (89%)
Frame = +2 / +2
Query: 2 PMSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKS 181
PMSRI+DAVYRILRVKFTMGLFE+PY D SL GELGKQEHR+LAREAVRKSLVLLKNGKS
Sbjct: 1100 PMSRINDAVYRILRVKFTMGLFESPYADPSLVGELGKQEHRDLAREAVRKSLVLLKNGKS 1279
Query: 182 SYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAGTTIL 331
+ PLLPLPKKAGKILVAGSHA+DLGNQCGGWTITWQG +GN T +
Sbjct: 1280 ASTPLLPLPKKAGKILVAGSHADDLGNQCGGWTITWQGQTGNDKTAGTTI 1429
Score = 189 bits (409), Expect(3) = 1e-080
Identities = 78/97 (80%), Positives = 81/97 (83%)
Frame = -3 / -3
Query: 291 CHVIVHPPHWLPRSLAWLPATRILPAFFGRGSNGA*EDLPFFRRTRDFLTASRASSRCSC 112
CHV+VHPPHWLP+S AWLPATRILPAFFGRG+NG D PFF T DFLTASRA SRCSC
Sbjct: 1389 CHVMVHPPHWLPKSSAWLPATRILPAFFGRGNNGVEADFPFFSNTNDFLTASRARSRCSC 1210
Query: 111 FPSSPARLESG*GFSNRPMVNLTRRIL*TASSILLMG 1
FPSSP RL S *G SNRPMVN T RIL*TAS ILLMG
Sbjct: 1209 FPSSPTRLGSA*GLSNRPMVNFTLRIL*TASLILLMG 1099
Score = 119 bits (255), Expect(2) = 7e-086
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +2 / +2
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 1496 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLV 1675
Query: 572 VE 577
VE
Sbjct: 1676 VE 1681
Score = 112 bits (241), Expect(3) = 1e-080
Identities = 49/60 (81%), Positives = 50/60 (83%)
Frame = -3 / -3
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 1680 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 1501
Score = 92.2 bits (196), Expect(2) = 7e-033
Identities = 45/102 (44%), Positives = 55/102 (53%)
Frame = -2 / -2
Query: 307 VAAASLPRDRPPPXXXXXXXXXXXXXXDLAGLLREGQQRSVGGLAVLQENQGFPDGFARQ 128
VA SLP D P DL GLL E QQ S G ++ Q++Q PDG +
Sbjct: 1405 VAGLSLPCDGPSSALVAQVVGVASSDEDLTGLLWERQQWSGGRFSIFQQHQ*LPDGLTSK 1226
Query: 127 FPVFLLPELAGEARVGVRVLEQAHGELDTKDPVDGVVDSAHG 2
VFLLPE EA V + L+ HGEL ++PVD +VDSAHG
Sbjct: 1225 ITVFLLPEFTNEAWVSIGALK*THGELHPENPVDSIVDSAHG 1100
Score = 67.2 bits (141), Expect(2) = 7e-033
Identities = 33/56 (58%), Positives = 34/56 (60%)
Frame = -2 / -2
Query: 577 LHHQXPAGDEDHNALGXSAHXXXXXXXXXXXRQVQVVAERLRVWRLPDHHDRVVVL 410
LHHQ PAGDE H+A AH RQVQVVAERL V L DHHD VVVL
Sbjct: 1681 LHHQRPAGDEHHHAPDALAHVLDHRGAGRRDRQVQVVAERLSVRWLADHHDGVVVL 1514
Score = 59.9 bits (125), Expect(4) = 7e-025
Identities = 31/66 (46%), Positives = 37/66 (56%)
Frame = +3 / +3
Query: 3 P*AESTTPSTGSFVSSSPWACSRTLTPTLASPASSGSKNTGNWRAKPSGNPWFS*RTASP 182
P*AEST STG SSPW R T AS +SGS+NT +PSG+ W *+ +
Sbjct: 1101 P*AESTMLSTGFSG*SSPWVYLRAPMLTQASLVNSGSRNTVILLVRPSGSHWCC*KMENL 1280
Query: 183 PTLRCC 200
P L CC
Sbjct: 1281 PPLHCC 1298
Score = 55.8 bits (116), Expect(4) = 7e-025
Identities = 22/36 (61%), Positives = 30/36 (83%)
Frame = +2 / +2
Query: 296 SSGNTTAGTTILSGIEATVDPSTQVVYSESPDSGVL 403
+ + TAGTTILS I++TVDPST+VV+SE+PDS +
Sbjct: 1397 TGNDKTAGTTILSAIKSTVDPSTEVVFSENPDSAAV 1504
Score = 35.2 bits (71), Expect(3) = 1e-080
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / -3
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 1506 STAALSGFSEKTTSVLGSTVDLIAERI 1426
Score = 27.0 bits (53), Expect(4) = 7e-025
Identities = 10/11 (90%), Positives = 10/11 (90%)
Frame = +1 / +1
Query: 544 GPHLRQAAGGG 576
G HLRQAAGGG
Sbjct: 1648 GAHLRQAAGGG 1680
Score = 25.2 bits (49), Expect(4) = 7e-025
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = +1 / +1
Query: 469 QPEPDDPGAR 498
QPEPDDP AR
Sbjct: 1573 QPEPDDPCAR 1602
>gb|AV925468.1|AV925468 AV925468 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd24f16 5', mRNA sequence
Length = 621
Score = 220 bits (477), Expect(2) = 7e-078
Identities = 91/110 (82%), Positives = 98/110 (89%)
Frame = +2 / +2
Query: 2 PMSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKS 181
PMSRI+DAVYRILRVKFTMGLFE+PY D SL GELGKQEHR+LAREAVRKSLVLLKNGKS
Sbjct: 92 PMSRINDAVYRILRVKFTMGLFESPYADPSLVGELGKQEHRDLAREAVRKSLVLLKNGKS 271
Query: 182 SYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAGTTIL 331
+ PLLPLPKKAGKILVAGSHA+DLGNQCGGWTITWQG +GN T +
Sbjct: 272 ASTPLLPLPKKAGKILVAGSHADDLGNQCGGWTITWQGQTGNDKTAGTTI 421
Score = 189 bits (409), Expect(3) = 2e-072
Identities = 78/97 (80%), Positives = 81/97 (83%)
Frame = -3 / -1
Query: 291 CHVIVHPPHWLPRSLAWLPATRILPAFFGRGSNGA*EDLPFFRRTRDFLTASRASSRCSC 112
CHV+VHPPHWLP+S AWLPATRILPAFFGRG+NG D PFF T DFLTASRA SRCSC
Sbjct: 381 CHVMVHPPHWLPKSSAWLPATRILPAFFGRGNNGVEADFPFFSNTNDFLTASRARSRCSC 202
Query: 111 FPSSPARLESG*GFSNRPMVNLTRRIL*TASSILLMG 1
FPSSP RL S *G SNRPMVN T RIL*TAS ILLMG
Sbjct: 201 FPSSPTRLGSA*GLSNRPMVNFTLRIL*TASLILLMG 91
Score = 92.7 bits (197), Expect(2) = 1e-027
Identities = 45/102 (44%), Positives = 55/102 (53%)
Frame = -2 / -3
Query: 307 VAAASLPRDRPPPXXXXXXXXXXXXXXDLAGLLREGQQRSVGGLAVLQENQGFPDGFARQ 128
VA SLP D P DL GLL E QQ S G ++ Q++Q PDG +
Sbjct: 397 VAGLSLPCDGPSSALVAQVVSVASSDEDLTGLLWERQQWSGGRFSIFQQHQ*LPDGLTSK 218
Query: 127 FPVFLLPELAGEARVGVRVLEQAHGELDTKDPVDGVVDSAHG 2
VFLLPE EA V + L+ HGEL ++PVD +VDSAHG
Sbjct: 217 IAVFLLPEFTNEAWVSIGALK*THGELHPENPVDSIVDSAHG 92
Score = 92.2 bits (196), Expect(2) = 7e-078
Identities = 37/44 (84%), Positives = 40/44 (90%)
Frame = +2 / +2
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVC 523
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC
Sbjct: 488 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVC 619
Score = 85.4 bits (181), Expect(3) = 2e-072
Identities = 36/42 (85%), Positives = 37/42 (88%)
Frame = -3 / -1
Query: 522 HTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 618 HTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 493
Score = 61.2 bits (128), Expect(3) = 2e-022
Identities = 31/66 (46%), Positives = 37/66 (56%)
Frame = +3 / +3
Query: 3 P*AESTTPSTGSFVSSSPWACSRTLTPTLASPASSGSKNTGNWRAKPSGNPWFS*RTASP 182
P*AEST STG SSPW R T AS +SGS+NT +PSG+ W *+ +
Sbjct: 93 P*AESTMLSTGFSG*SSPWVYLRAPMLTQASLVNSGSRNTAILLVRPSGSHWCC*KMENL 272
Query: 183 PTLRCC 200
P L CC
Sbjct: 273 PPLHCC 290
Score = 55.8 bits (116), Expect(3) = 2e-022
Identities = 22/36 (61%), Positives = 30/36 (83%)
Frame = +2 / +2
Query: 296 SSGNTTAGTTILSGIEATVDPSTQVVYSESPDSGVL 403
+ + TAGTTILS I++TVDPST+VV+SE+PDS +
Sbjct: 389 TGNDKTAGTTILSAIKSTVDPSTEVVFSENPDSAAV 496
Score = 49.4 bits (102), Expect(2) = 1e-027
Identities = 25/50 (50%), Positives = 27/50 (54%)
Frame = -2 / -3
Query: 448 WRLPDHHDRVVVLVGQHAAVRALRVHHLRAGVHGGLDPGEDRRAGSSVAA 299
W H VV+ AVR L HLRAGV GGLD +DRR GS V A
Sbjct: 541 WLADHHDGVVVLAAVNGGAVRVL*EDHLRAGVDGGLDRRKDRRPGSFVVA 392
Score = 35.2 bits (71), Expect(3) = 2e-072
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / -1
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 498 STAALSGFSEKTTSVLGSTVDLIAERI 418
Score = 25.2 bits (49), Expect(3) = 2e-022
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = +1 / +1
Query: 469 QPEPDDPGAR 498
QPEPDDP AR
Sbjct: 565 QPEPDDPCAR 594
>gb|AL504655.1|AL504655 AL504655 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW05P08V 5', mRNA sequence
Length = 700
Score = 220 bits (477), Expect(2) = 7e-070
Identities = 91/110 (82%), Positives = 98/110 (89%)
Frame = +2 / +2
Query: 2 PMSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKS 181
PMSRI+DAVYRILRVKFTMGLFE+PY D SL GELGKQEHR+LAREAVRKSLVLLKNGKS
Sbjct: 182 PMSRINDAVYRILRVKFTMGLFESPYADPSLVGELGKQEHRDLAREAVRKSLVLLKNGKS 361
Query: 182 SYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAGTTIL 331
+ PLLPLPKKAGKILVAGSHA+DLGNQCGGWTITWQG +GN T +
Sbjct: 362 ASTPLLPLPKKAGKILVAGSHADDLGNQCGGWTITWQGQTGNDKTAGTTI 511
Score = 189 bits (409), Expect(3) = 3e-067
Identities = 78/97 (80%), Positives = 81/97 (83%)
Frame = -3 / -2
Query: 291 CHVIVHPPHWLPRSLAWLPATRILPAFFGRGSNGA*EDLPFFRRTRDFLTASRASSRCSC 112
CHV+VHPPHWLP+S AWLPATRILPAFFGRG+NG D PFF T DFLTASRA SRCSC
Sbjct: 471 CHVMVHPPHWLPKSSAWLPATRILPAFFGRGNNGVEADFPFFSNTNDFLTASRARSRCSC 292
Query: 111 FPSSPARLESG*GFSNRPMVNLTRRIL*TASSILLMG 1
FPSSP RL S *G SNRPMVN T RIL*TAS ILLMG
Sbjct: 291 FPSSPTRLGSA*GLSNRPMVNFTLRIL*TASLILLMG 181
Score = 92.2 bits (196), Expect(3) = 5e-029
Identities = 45/102 (44%), Positives = 55/102 (53%)
Frame = -2 / -1
Query: 307 VAAASLPRDRPPPXXXXXXXXXXXXXXDLAGLLREGQQRSVGGLAVLQENQGFPDGFARQ 128
VA SLP D P DL GLL E QQ S G ++ Q++Q PDG +
Sbjct: 487 VAGLSLPCDGPSSALVAQVVGVASSDEDLTGLLWERQQWSGGRFSIFQQHQ*LPDGLTSK 308
Query: 127 FPVFLLPELAGEARVGVRVLEQAHGELDTKDPVDGVVDSAHG 2
VFLLPE EA V + L+ HGEL ++PVD +VDSAHG
Sbjct: 307 ITVFLLPEFTNEAWVSIGALK*THGELHPENPVDSIVDSAHG 182
Score = 65.3 bits (137), Expect(2) = 7e-070
Identities = 26/29 (89%), Positives = 26/29 (89%)
Frame = +2 / +1
Query: 416 DYAIVVVGEPPYAETFGDNLNLTIPAPGP 502
DYAIVVVGEPPYAETFGDNLNLTIP P
Sbjct: 604 DYAIVVVGEPPYAETFGDNLNLTIPCARP 690
Score = 59.9 bits (125), Expect(3) = 5e-021
Identities = 31/66 (46%), Positives = 37/66 (56%)
Frame = +3 / +3
Query: 3 P*AESTTPSTGSFVSSSPWACSRTLTPTLASPASSGSKNTGNWRAKPSGNPWFS*RTASP 182
P*AEST STG SSPW R T AS +SGS+NT +PSG+ W *+ +
Sbjct: 183 P*AESTMLSTGFSG*SSPWVYLRAPMLTQASLVNSGSRNTVILLVRPSGSHWCC*KMENL 362
Query: 183 PTLRCC 200
P L CC
Sbjct: 363 PPLHCC 380
Score = 59.4 bits (124), Expect(3) = 3e-067
Identities = 26/31 (83%), Positives = 27/31 (87%)
Frame = -3 / -3
Query: 501 GPGAGIVRFRLSPNVSAYGGSPTTTIA*SYL 409
G GIVRFRLSPNVSAYGGSPTTT+A*S L
Sbjct: 689 GRAQGIVRFRLSPNVSAYGGSPTTTMA*SVL 597
Score = 55.3 bits (115), Expect(3) = 5e-021
Identities = 22/33 (66%), Positives = 29/33 (87%)
Frame = +2 / +2
Query: 296 SSGNTTAGTTILSGIEATVDPSTQVVYSESPDS 394
+ + TAGTTILS I++TVDPST+VV+SE+PDS
Sbjct: 479 TGNDKTAGTTILSAIKSTVDPSTEVVFSENPDS 577
Score = 43.9 bits (90), Expect(3) = 3e-067
Identities = 21/32 (65%), Positives = 22/32 (68%)
Frame = -2 / -1
Query: 394 AVRALRVHHLRAGVHGGLDPGEDRRAGSSVAA 299
AVR L HLRAGV GGLD +DRR GS V A
Sbjct: 577 AVRVL*EDHLRAGVDGGLDRRKDRRPGSFVVA 482
Score = 36.6 bits (74), Expect(3) = 5e-029
Identities = 17/22 (77%), Positives = 17/22 (77%)
Frame = -2 / -2
Query: 481 QVQVVAERLRVWRLPDHHDRVV 416
QVQVVAERL V L DHHD VV
Sbjct: 669 QVQVVAERLSVRWLADHHDGVV 604
Score = 35.2 bits (71), Expect(3) = 5e-029
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / -2
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 588 STXALSGFSEKTTSVLGSTVDLIAERI 508
Score = 22.5 bits (43), Expect(3) = 5e-021
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = +1 / +3
Query: 469 QPEPDDP 489
QPEPDDP
Sbjct: 657 QPEPDDP 677
>gb|BF257182.2|BF257182 HVSMEf0012B20f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0012B20f, mRNA sequence
Length = 537
Score = 219 bits (476), Expect(2) = 3e-068
Identities = 91/110 (82%), Positives = 98/110 (89%)
Frame = +2 / +2
Query: 2 PMSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKS 181
PMSRI+DAVYRILRVKFTMGLFE+PY D SL GELGKQEHR+LAREAVRKSLVLLKNGKS
Sbjct: 53 PMSRINDAVYRILRVKFTMGLFESPYADPSLIGELGKQEHRDLAREAVRKSLVLLKNGKS 232
Query: 182 SYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAGTTIL 331
+ PLLPLPKKAGKILVAGSHA+DLGNQCGGWTITWQG +GN T +
Sbjct: 233 ASTPLLPLPKKAGKILVAGSHADDLGNQCGGWTITWQGQTGNDKTAGTTI 382
Score = 188 bits (408), Expect(3) = 3e-064
Identities = 78/97 (80%), Positives = 81/97 (83%)
Frame = -3 / -1
Query: 291 CHVIVHPPHWLPRSLAWLPATRILPAFFGRGSNGA*EDLPFFRRTRDFLTASRASSRCSC 112
CHV+VHPPHWLP+S AWLPATRILPAFFGRG+NG D PFF T DFLTASRA SRCSC
Sbjct: 342 CHVMVHPPHWLPKSSAWLPATRILPAFFGRGNNGVEADFPFFSNTNDFLTASRARSRCSC 163
Query: 111 FPSSPARLESG*GFSNRPMVNLTRRIL*TASSILLMG 1
FPSSP RL S *G SNRPMVN T RIL*TAS ILLMG
Sbjct: 162 FPSSPMRLGSA*GLSNRPMVNFTLRIL*TASLILLMG 52
Score = 92.2 bits (196), Expect(2) = 1e-027
Identities = 45/102 (44%), Positives = 55/102 (53%)
Frame = -2 / -3
Query: 307 VAAASLPRDRPPPXXXXXXXXXXXXXXDLAGLLREGQQRSVGGLAVLQENQGFPDGFARQ 128
VA SLP D P DL GLL E QQ S G ++ Q++Q PDG +
Sbjct: 358 VAGLSLPCDGPSSALVAQVVGVASSDEDLTGLLWERQQWSGGRFSIFQQHQ*LPDGLTSK 179
Query: 127 FPVFLLPELAGEARVGVRVLEQAHGELDTKDPVDGVVDSAHG 2
VFLLPE EA V + L+ HGEL ++PVD +VDSAHG
Sbjct: 178 ITVFLLPEFTNEAWVSIGALK*THGELHPENPVDSIVDSAHG 53
Score = 60.3 bits (126), Expect(2) = 3e-068
Identities = 24/29 (82%), Positives = 26/29 (89%)
Frame = +2 / +2
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLN 478
+ V + KYDYAIVVVGEPPYAETFGDNLN
Sbjct: 449 AAVDSGKYDYAIVVVGEPPYAETFGDNLN 535
Score = 58.5 bits (122), Expect(3) = 3e-064
Identities = 25/28 (89%), Positives = 26/28 (92%)
Frame = -3 / -1
Query: 480 RFRLSPNVSAYGGSPTTTIA*SYLSAST 397
RFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 537 RFRLSPNVSAYGGSPTTTMA*SYLPLST 454
Score = 49.4 bits (102), Expect(2) = 1e-027
Identities = 25/50 (50%), Positives = 27/50 (54%)
Frame = -2 / -3
Query: 448 WRLPDHHDRVVVLVGQHAAVRALRVHHLRAGVHGGLDPGEDRRAGSSVAA 299
W H VV+ AVR L HLRAGV GGLD +DRR GS V A
Sbjct: 502 WLADHHDGVVVLAAVNGGAVRVL*EDHLRAGVDGGLDRRKDRRPGSFVVA 353
Score = 35.2 bits (71), Expect(3) = 3e-064
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / -1
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 459 STAALSGFSEKTTSVLGSTVDLIAERI 379
>gb|AV914506.1|AV914506 AV914506 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags6k08 5', mRNA sequence
Length = 544
Score = 133 bits (286), Expect(4) = 6e-068
Identities = 54/71 (76%), Positives = 59/71 (83%)
Frame = +2 / +2
Query: 119 HRELAREAVRKSLVLLKNGKSSYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGS 298
HR+LAREA RKSLVLLKNGKS+ PLLPLPKKAGKILVAGSHA+ LGNQCGGWTITW G
Sbjct: 41 HRDLAREAXRKSLVLLKNGKSASTPLLPLPKKAGKILVAGSHADXLGNQCGGWTITWXGQ 220
Query: 299 SGNTTAGTTIL 331
+GN T +
Sbjct: 221 TGNDXTAGTTI 253
Score = 114 bits (244), Expect(3) = 2e-054
Identities = 43/57 (75%), Positives = 46/57 (80%)
Frame = -3 / -2
Query: 288 HVIVHPPHWLPRSLAWLPATRILPAFFGRGSNGA*EDLPFFRRTRDFLTASRASSRC 118
HV+VHPPHWLP+ AWLPATRILPAFFGRG+NG D PFF T DFL ASRA SRC
Sbjct: 210 HVMVHPPHWLPKXSAWLPATRILPAFFGRGNNGVEADFPFFSNTNDFLXASRARSRC 40
Score = 107 bits (230), Expect(4) = 6e-068
Identities = 44/57 (77%), Positives = 48/57 (84%)
Frame = +2 / +2
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGR 562
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CV LISGR
Sbjct: 320 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVXXLISGR 490
Score = 100 bits (214), Expect(3) = 2e-054
Identities = 43/55 (78%), Positives = 44/55 (80%)
Frame = -3 / -2
Query: 561 LPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
LPEM THL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 489 LPEMSXXTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 325
Score = 49.8 bits (103), Expect(4) = 6e-068
Identities = 19/29 (65%), Positives = 21/29 (72%)
Frame = +1 / +1
Query: 313 CRHDDPLRDRGHRGPQHAGGVLGEPGQRR 399
CR DDP D+ HR PQH GG+L EP QRR
Sbjct: 238 CRDDDPFGDQVHRRPQHGGGLLREP*QRR 324
Score = 35.2 bits (71), Expect(3) = 2e-054
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / -2
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 330 STAALSGFSEKTTSVLGSTVDLIAERI 250
Score = 25.2 bits (49), Expect(4) = 6e-068
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +1 / +1
Query: 544 GPHLRQAAGGG 576
G HLRQA GGG
Sbjct: 472 GAHLRQAXGGG 504
>gb|BU977331.1|BU977331 HA11D20r HA Hordeum vulgare subsp. vulgare cDNA clone HA11D20
5-PRIME, mRNA sequence
Length = 486
Score = 220 bits (477), Expect(2) = 7e-062
Identities = 91/110 (82%), Positives = 98/110 (89%)
Frame = +2 / +2
Query: 2 PMSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKS 181
PMSRI+DAVYRILRVKFTMGLFE+PY D SL GELGKQEHR+LAREAVRKSLVLLKNGKS
Sbjct: 29 PMSRINDAVYRILRVKFTMGLFESPYADPSLVGELGKQEHRDLAREAVRKSLVLLKNGKS 208
Query: 182 SYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAGTTIL 331
+ PLLPLPKKAGKILVAGSHA+DLGNQCGGWTITWQG +GN T +
Sbjct: 209 ASTPLLPLPKKAGKILVAGSHADDLGNQCGGWTITWQGQTGNDKTAGTTI 358
Score = 189 bits (409), Expect(3) = 3e-058
Identities = 78/97 (80%), Positives = 81/97 (83%)
Frame = -3 / -1
Query: 291 CHVIVHPPHWLPRSLAWLPATRILPAFFGRGSNGA*EDLPFFRRTRDFLTASRASSRCSC 112
CHV+VHPPHWLP+S AWLPATRILPAFFGRG+NG D PFF T DFLTASRA SRCSC
Sbjct: 318 CHVMVHPPHWLPKSSAWLPATRILPAFFGRGNNGVEADFPFFSNTNDFLTASRARSRCSC 139
Query: 111 FPSSPARLESG*GFSNRPMVNLTRRIL*TASSILLMG 1
FPSSP RL S *G SNRPMVN T RIL*TAS ILLMG
Sbjct: 138 FPSSPTRLGSA*GLSNRPMVNFTLRIL*TASLILLMG 28
Score = 92.2 bits (196), Expect(2) = 1e-027
Identities = 45/102 (44%), Positives = 55/102 (53%)
Frame = -2 / -3
Query: 307 VAAASLPRDRPPPXXXXXXXXXXXXXXDLAGLLREGQQRSVGGLAVLQENQGFPDGFARQ 128
VA SLP D P DL GLL E QQ S G ++ Q++Q PDG +
Sbjct: 334 VAGLSLPCDGPSSALVAQVVGVASSDEDLTGLLWERQQWSGGRFSIFQQHQ*LPDGLTSK 155
Query: 127 FPVFLLPELAGEARVGVRVLEQAHGELDTKDPVDGVVDSAHG 2
VFLLPE EA V + L+ HGEL ++PVD +VDSAHG
Sbjct: 154 ITVFLLPEFTNEAWVSIGALK*THGELHPENPVDSIVDSAHG 29
Score = 49.4 bits (102), Expect(2) = 1e-027
Identities = 25/50 (50%), Positives = 27/50 (54%)
Frame = -2 / -3
Query: 448 WRLPDHHDRVVVLVGQHAAVRALRVHHLRAGVHGGLDPGEDRRAGSSVAA 299
W H VV+ AVR L HLRAGV GGLD +DRR GS V A
Sbjct: 478 WLADHHDGVVVLAAVNGGAVRVL*EDHLRAGVDGGLDRRKDRRPGSFVVA 329
Score = 38.4 bits (78), Expect(2) = 7e-062
Identities = 15/20 (75%), Positives = 17/20 (85%)
Frame = +2 / +2
Query: 392 SGVLADKYDYAIVVVGEPPY 451
+ V + KYDYAIVVVGEPPY
Sbjct: 425 AAVDSGKYDYAIVVVGEPPY 484
Score = 38.0 bits (77), Expect(3) = 3e-058
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = -3 / -1
Query: 453 AYGGSPTTTIA*SYLSAST 397
AYGGSPTTT+A*SYL ST
Sbjct: 486 AYGGSPTTTMA*SYLPLST 430
Score = 35.2 bits (71), Expect(3) = 3e-058
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / -1
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 435 STAALSGFSEKTTSVLGSTVDLIAERI 355
>gb|CB883402.1|CB883402 HQ02C08w HQ Hordeum vulgare subsp. vulgare cDNA clone HQ02C08
3-PRIME, mRNA sequence
Length = 665
Score = 219 bits (476), Expect = 2e-056
Identities = 94/133 (70%), Positives = 107/133 (80%)
Frame = +2 / +1
Query: 5 MSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKSS 184
MSRIDDAV RILRVKFTMGLF+NP D S A +LGK+EHRELAREAVRKSLVLLKNG +
Sbjct: 181 MSRIDDAVSRILRVKFTMGLFDNPLADLSFADQLGKKEHRELAREAVRKSLVLLKNGNTP 360
Query: 185 YAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAGTTILSGIEATVDPST 364
LPLPKKA KILVAGSHA++LG QCGGW+I W G SG+ TAGTTIL I++TV +T
Sbjct: 361 NQQFLPLPKKASKILVAGSHASNLGYQCGGWSIQWMGGSGDITAGTTILDAIKSTVGDAT 540
Query: 365 QVVYSESPDSGVL 403
VVYSE+PD +
Sbjct: 541 PVVYSENPDDSFM 579
Score = 81.3 bits (172), Expect(3) = 2e-036
Identities = 38/58 (65%), Positives = 43/58 (74%)
Frame = -3 / -1
Query: 177 LPFFRRTRDFLTASRASSRCSCFPSSPARLESG*GFSNRPMVNLTRRIL*TASSILLM 4
LPFF RT DFLTAS A+S CS FPS A+L S G S RP+VN TRR+ TASSILL+
Sbjct: 353 LPFFNRTSDFLTASLANSLCSFFPS*SAKLRSAKGLSKRPIVNFTRRMRLTASSILLI 180
Score = 69.0 bits (145), Expect(3) = 2e-036
Identities = 36/66 (54%), Positives = 38/66 (57%)
Frame = -3 / -1
Query: 390 SGLSEYTTCVLGSTVASIPERIXXXXXXXXXXPCHVIVHPPHWLPRSLAWLPATRILPAF 211
SG SE TT V TV + RI P H I HPPHW P+ LA LPATRIL AF
Sbjct: 566 SGFSE*TTGVASPTVDFMASRIVVPAVISPLPPIH*IDHPPHW*PKLLA*LPATRILLAF 387
Query: 210 FGRGSN 193
FGRG N
Sbjct: 386 FGRGRN 369
Score = 38.9 bits (79), Expect(3) = 2e-036
Identities = 18/22 (81%), Positives = 18/22 (81%)
Frame = -3 / -1
Query: 486 IVRFRLSPNVSAYGGSPTTTIA 421
IVR LSP VSAYG SPTTTIA
Sbjct: 665 IVRSVLSPTVSAYGVSPTTTIA 600
>gb|AF102868.1| Hordeum vulgare subsp. vulgare beta-D-glucan exohydrolase isoenzyme
ExoI mRNA, complete cds
Length = 2161
Score = 129 bits (278), Expect(3) = 3e-055
Identities = 53/85 (62%), Positives = 66/85 (77%)
Frame = +2 / +2
Query: 140 AVRKSLVLLKNGKSSYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAG 319
A + ++L S+ APLLPLPKKA KILVAGSHA++LG QCGGWTI WQG +G TT G
Sbjct: 1247 ARKSLVLLKNGKTSTDAPLLPLPKKAPKILVAGSHADNLGYQCGGWTIEWQGDTGRTTVG 1426
Query: 320 TTILSGIEATVDPSTQVVYSESPDS 394
TTIL ++A VDPST VV++E+PD+
Sbjct: 1427 TTILEAVKAAVDPSTVVVFAENPDA 1501
Score = 120 bits (257), Expect(2) = 2e-030
Identities = 51/61 (83%), Positives = 56/61 (91%)
Frame = +2 / +2
Query: 2 PMSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKS 181
PMSRIDDAV RILRVKFTMGLFENPY D ++A +LGKQEHR+LAREA RKSLVLLKNGK+
Sbjct: 1106 PMSRIDDAVTRILRVKFTMGLFENPYADPAMAEQLGKQEHRDLAREAARKSLVLLKNGKT 1285
Query: 182 S 184
S
Sbjct: 1286 S 1288
Score = 93.1 bits (198), Expect(3) = 8e-051
Identities = 45/66 (68%), Positives = 46/66 (69%)
Frame = -3 / -2
Query: 198 SNGA*EDLPFFRRTRDFLTASRASSRCSCFPSSPARLESG*GFSNRPMVNLTRRIL*TAS 19
S + E LPFF T DFL AS A S CSCFPS A S *GFS RPMVNLTRRI TAS
Sbjct: 1302 SGASVEVLPFFSSTSDFLAASLAKSLCSCFPSCSAMAGSA*GFSKRPMVNLTRRIRVTAS 1123
Query: 18 SILLMG 1
SILLMG
Sbjct: 1122 SILLMG 1105
Score = 88.1 bits (187), Expect(3) = 3e-055
Identities = 36/55 (65%), Positives = 41/55 (74%)
Frame = +2 / +2
Query: 413 YDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLVVE 577
+ YAIV VGE PY ET GDNLNLTIP PG S Q+VCG +C VLISGR +VV+
Sbjct: 1523 FSYAIVAVGEHPYTETKGDNLNLTIPEPGLSTVQAVCGGVRCATVLISGRPVVVQ 1687
Score = 77.2 bits (163), Expect(3) = 8e-051
Identities = 36/68 (52%), Positives = 39/68 (57%)
Frame = -3 / -2
Query: 390 SGLSEYTTCVLGSTVASIPERIXXXXXXXXXXPCHVIVHPPHWLPRSLAWLPATRILPAF 211
SG S TT V GST A R+ PCH +V PPHW PR AWLPATRIL AF
Sbjct: 1497 SGFSANTTTVDGSTAAFTASRMVVPTVVRPVSPCHSMVQPPHW*PRLSAWLPATRILGAF 1318
Query: 210 FGRGSNGA 187
G G +GA
Sbjct: 1317 LGSGRSGA 1294
Score = 76.7 bits (162), Expect(3) = 1e-024
Identities = 34/59 (57%), Positives = 39/59 (66%)
Frame = -2 / -1
Query: 178 LAVLQENQGFPDGFARQFPVFLLPELAGEARVGVRVLEQAHGELDTKDPVDGVVDSAHG 2
LAVLQ++Q P RQ PV LL L G RV V VLE+ HGELD +DP G+VD AHG
Sbjct: 1282 LAVLQQHQRLPRRLPRQIPVLLLS*LLGHGRVSVGVLEETHGELDPQDPGHGIVDPAHG 1106
Score = 67.2 bits (141), Expect(3) = 8e-051
Identities = 30/52 (57%), Positives = 34/52 (65%)
Frame = -3 / -2
Query: 573 TTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA* 418
TT+ P M T H PHT L PG+G+VRFRLSP VS YG SPT T+A*
Sbjct: 1683 TTTGRPLMSTVAHRTPPHTACTVLRPGSGMVRFRLSPLVSVYGCSPTATMA* 1528
Score = 37.1 bits (75), Expect(3) = 3e-055
Identities = 14/20 (70%), Positives = 16/20 (80%)
Frame = +1 / +1
Query: 1 PHEQNRRRRLQDPSCQVHHG 60
PHEQ+RR R DP+ QVHHG
Sbjct: 1105 PHEQDRRCRDPDPAGQVHHG 1164
Score = 36.6 bits (74), Expect(3) = 1e-024
Identities = 21/53 (39%), Positives = 23/53 (43%)
Frame = -2 / -1
Query: 577 LHHQXPAGDEDHNALGXSAHXXXXXXXXXXXRQVQVVAERLRVWRLPDHHDRV 419
LHH P DE A +AH QVQVVA LRV L +D V
Sbjct: 1687 LHHHGPPADEHRRAPHAAAHRLHGAQAGLRDGQVQVVALGLRVRVLAHGNDGV 1529
Score = 35.7 bits (72), Expect(2) = 2e-030
Identities = 16/35 (45%), Positives = 16/35 (45%)
Frame = +1 / +1
Query: 469 QPEPDDPGARAEXXXXXXXXXXXXCGPHLRQAAGG 573
QPEPD PGAR E G H R A GG
Sbjct: 1579 QPEPDHPGARPEHRAGGVRRRAVRDGAHQRAARGG 1683
Score = 35.7 bits (72), Expect(3) = 1e-024
Identities = 24/70 (34%), Positives = 28/70 (40%)
Frame = -2 / -1
Query: 391 VRALRVHHLRAGVHGGLDPGEDRRAGSSVAAASLPRDRPPPXXXXXXXXXXXXXXDLAGL 212
V L HH R VHG L +D A A +LP D P DL L
Sbjct: 1498 VGVLGEHHDRRRVHGRLHRLQDGGAHGGAARVALPLDGPAAALVAQVVGVAPGDQDLGRL 1319
Query: 211 LREGQQRSVG 182
L + Q+R VG
Sbjct: 1318 LGQRQKRRVG 1289
>gb|BE411093.1|BE411093 ISC002.C07F990406 ITEC ISC Barley Leaf Library Hordeum vulgare
subsp. vulgare cDNA clone ISC002.C07, mRNA sequence
Length = 426
Score = 213 bits (461), Expect = 2e-054
Identities = 88/107 (82%), Positives = 95/107 (88%)
Frame = +2 / +3
Query: 11 RIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKSSYA 190
RI+DAVYRILRVKFTMGLFE+PY D SL GELGKQEHR+LAREAVRKSLVLLKNGKS+
Sbjct: 3 RINDAVYRILRVKFTMGLFESPYADPSLVGELGKQEHRDLAREAVRKSLVLLKNGKSAST 182
Query: 191 PLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAGTTIL 331
PLLPLPKKAGKILVAGSHA+DLGNQCGGWTITWQG +GN T +
Sbjct: 183 PLLPLPKKAGKILVAGSHADDLGNQCGGWTITWQGQTGNDKTAGTTI 323
Score = 182 bits (394), Expect(2) = 1e-049
Identities = 75/94 (79%), Positives = 78/94 (82%)
Frame = -3 / -3
Query: 291 CHVIVHPPHWLPRSLAWLPATRILPAFFGRGSNGA*EDLPFFRRTRDFLTASRASSRCSC 112
CHV+VHPPHWLP+S AWLPATRILPAFFGRG+NG D PFF T DFLTASRA SRCSC
Sbjct: 283 CHVMVHPPHWLPKSSAWLPATRILPAFFGRGNNGVEADFPFFSNTNDFLTASRARSRCSC 104
Query: 111 FPSSPARLESG*GFSNRPMVNLTRRIL*TASSIL 10
FPSSP RL S *G SNRPMVN T RIL*TAS IL
Sbjct: 103 FPSSPTRLGSA*GLSNRPMVNFTLRIL*TASLIL 2
Score = 32.5 bits (65), Expect(2) = 1e-049
Identities = 18/44 (40%), Positives = 21/44 (47%)
Frame = -2 / -2
Query: 430 HDRVVVLVGQHAAVRALRVHHLRAGVHGGLDPGEDRRAGSSVAA 299
H VV L +AA+ V GGLD +DRR GS V A
Sbjct: 425 HXGVVELAAXNAALSGFSRRPXPCWVDGGLDRRKDRRPGSFVVA 294
>gb|BU996833.1|BU996833 HM14O21r HM Hordeum vulgare subsp. vulgare cDNA clone HM14O21
5-PRIME, mRNA sequence
Length = 532
Score = 85.4 bits (181), Expect(3) = 4e-052
Identities = 35/44 (79%), Positives = 40/44 (90%)
Frame = +2 / +3
Query: 53 TMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKSS 184
TMGLFENPY D ++A +LGKQEHR+LAREA RKSLVLLKNGK+S
Sbjct: 21 TMGLFENPYADPAMAEQLGKQEHRDLAREAARKSLVLLKNGKTS 152
Score = 79.9 bits (169), Expect(3) = 4e-052
Identities = 28/44 (63%), Positives = 36/44 (81%)
Frame = +2 / +1
Query: 263 QCGGWTITWQGSSGNTTAGTTILSGIEATVDPSTQVVYSESPDS 394
+CGGWTI WQG +G TT GTTIL ++A VDPST VV++E+PD+
Sbjct: 235 ECGGWTIEWQGDTGRTTVGTTILEAVKAAVDPSTVVVFAENPDA 366
Score = 79.0 bits (167), Expect(3) = 4e-052
Identities = 32/48 (66%), Positives = 35/48 (72%)
Frame = +2 / +1
Query: 413 YDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLIS 556
+ YAIV VGE PY ET GDNLNLTIP PG S Q+VCG +C VLIS
Sbjct: 388 FSYAIVAVGEHPYTETKGDNLNLTIPEPGLSTVQAVCGGVRCATVLIS 531
Score = 64.4 bits (135), Expect(4) = 4e-035
Identities = 30/49 (61%), Positives = 31/49 (63%)
Frame = -3 / -1
Query: 198 SNGA*EDLPFFRRTRDFLTASRASSRCSCFPSSPARLESG*GFSNRPMV 52
S + E LPFF T DFL AS A S CSCFPS A S *GFS RPMV
Sbjct: 166 SGASVEVLPFFSSTSDFLAASLAKSLCSCFPSCSAMAGSA*GFSKRPMV 20
Score = 61.7 bits (129), Expect(4) = 4e-035
Identities = 27/45 (60%), Positives = 30/45 (66%)
Frame = -3 / -3
Query: 552 MRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA* 418
M T H PHT L PG+G+VRFRLSP VS YG SPT T+A*
Sbjct: 527 MSTVAHRTPPHTACTVLRPGSGMVRFRLSPLVSVYGCSPTATMA* 393
Score = 40.7 bits (83), Expect(4) = 4e-035
Identities = 16/24 (66%), Positives = 18/24 (75%)
Frame = -3 / -1
Query: 258 PRSLAWLPATRILPAFFGRGSNGA 187
PR AWLPATRI+ AF G G +GA
Sbjct: 229 PRLSAWLPATRIMGAFLGSGRSGA 158
Score = 35.2 bits (71), Expect(4) = 4e-035
Identities = 18/42 (42%), Positives = 20/42 (47%)
Frame = -3 / -3
Query: 390 SGLSEYTTCVLGSTVASIPERIXXXXXXXXXXPCHVIVHPPH 265
SG S TT V GST A R+ PCH +V PPH
Sbjct: 362 SGFSANTTTVDGSTAAFTASRMVVPTVVRPVSPCHSMVQPPH 237
>gb|AV919570.1|AV919570 AV919570 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags6k08 3', mRNA sequence
Length = 696
Score = 119 bits (255), Expect(4) = 2e-051
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +2 / -3
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 511 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLV 332
Query: 572 VE 577
VE
Sbjct: 331 VE 326
Score = 112 bits (241), Expect(4) = 5e-040
Identities = 49/60 (81%), Positives = 50/60 (83%)
Frame = -3 / +3
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 327 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 506
Score = 55.3 bits (115), Expect(4) = 2e-051
Identities = 22/31 (70%), Positives = 28/31 (90%)
Frame = +2 / -3
Query: 311 TAGTTILSGIEATVDPSTQVVYSESPDSGVL 403
TAGTTILS I++TVDPST+VV+SE+PDS +
Sbjct: 595 TAGTTILSAIKSTVDPSTEVVFSENPDSAAV 503
Score = 54.8 bits (114), Expect(4) = 2e-051
Identities = 18/20 (90%), Positives = 19/20 (95%)
Frame = +2 / -2
Query: 248 NDLGNQCGGWTITWQGSSGN 307
NDLGNQCGGWTITWQG +GN
Sbjct: 662 NDLGNQCGGWTITWQGQTGN 603
Score = 41.6 bits (85), Expect(4) = 5e-040
Identities = 12/16 (75%), Positives = 15/16 (93%)
Frame = -3 / +1
Query: 291 CHVIVHPPHWLPRSLA 244
CHV+VHPPHWLP+S +
Sbjct: 619 CHVMVHPPHWLPKSFS 666
Score = 35.2 bits (71), Expect(4) = 5e-040
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / +3
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 501 STAALSGFSEKTTSVLGSTVDLIAERI 581
Score = 30.7 bits (61), Expect(4) = 2e-051
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +2 / -1
Query: 215 AGKILVAGSHANDLG 259
AGKILVAGSHA LG
Sbjct: 696 AGKILVAGSHAERLG 652
Score = 29.3 bits (58), Expect(4) = 5e-040
Identities = 11/14 (78%), Positives = 11/14 (78%)
Frame = -3 / +2
Query: 258 PRSLAWLPATRILP 217
P AWLPATRILP
Sbjct: 653 PSRSAWLPATRILP 694
>gb|BI947544.1|BI947544 HVSMEl0005N11f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0005N11f, mRNA sequence
Length = 489
Score = 128 bits (275), Expect(3) = 1e-050
Identities = 52/65 (80%), Positives = 56/65 (86%)
Frame = +2 / +3
Query: 137 EAVRKSLVLLKNGKSSYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTA 316
EAVRKSLVLLKNGKS+ PLLPLPKKAGKILVAGSHA+DLGNQCGGWTITWQG +GN
Sbjct: 3 EAVRKSLVLLKNGKSACTPLLPLPKKAGKILVAGSHADDLGNQCGGWTITWQGQTGNDKT 182
Query: 317 GTTIL 331
T +
Sbjct: 183 AGTTI 197
Score = 110 bits (236), Expect(4) = 2e-048
Identities = 41/52 (78%), Positives = 44/52 (84%)
Frame = -3 / -3
Query: 291 CHVIVHPPHWLPRSLAWLPATRILPAFFGRGSNGA*EDLPFFRRTRDFLTAS 136
CHV+VHPPHWLP+S AWLPATRILPAFFGRG+NG D PFF T DFLTAS
Sbjct: 157 CHVMVHPPHWLPKSSAWLPATRILPAFFGRGNNGVQADFPFFSNTNDFLTAS 2
Score = 72.2 bits (152), Expect(3) = 1e-050
Identities = 31/43 (72%), Positives = 34/43 (79%)
Frame = +2 / +1
Query: 446 PYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLVV 574
P + G+NLNLTIPAPGPSV Q+VC KCVVVLISGR LVV
Sbjct: 319 PTLKPSGNNLNLTIPAPGPSVIQNVCKSVKCVVVLISGRPLVV 447
Score = 66.7 bits (140), Expect(4) = 2e-048
Identities = 28/37 (75%), Positives = 28/37 (75%)
Frame = -3 / -2
Query: 573 TTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSP 463
TTS LPEM TTTHL HT W T GPGAGIVRFRL P
Sbjct: 446 TTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLLP 336
Score = 49.4 bits (102), Expect(3) = 8e-021
Identities = 25/50 (50%), Positives = 27/50 (54%)
Frame = -2 / -2
Query: 448 WRLPDHHDRVVVLVGQHAAVRALRVHHLRAGVHGGLDPGEDRRAGSSVAA 299
W H VV+ AVR L HLRAGV GGLD +DRR GS V A
Sbjct: 317 WLADHHDGVVVLAAVNGGAVRVL*EDHLRAGVDGGLDRRKDRRPGSFVVA 168
Score = 46.2 bits (95), Expect(3) = 8e-021
Identities = 21/41 (51%), Positives = 23/41 (56%)
Frame = -2 / -1
Query: 574 HHQXPAGDEDHNALGXSAHXXXXXXXXXXXRQVQVVAERLR 452
HHQ PAGDE H+AL AH RQVQVVA R +
Sbjct: 447 HHQRPAGDEHHHALDALAHVLDHRGAGRRNRQVQVVARRFK 325
Score = 40.7 bits (83), Expect(3) = 8e-021
Identities = 22/56 (39%), Positives = 26/56 (46%)
Frame = -2 / -2
Query: 307 VAAASLPRDRPPPXXXXXXXXXXXXXXDLAGLLREGQQRSVGGLAVLQENQGFPDG 140
VA SLP D P DL GLL E QQ S G ++ Q++Q PDG
Sbjct: 173 VAGLSLPCDGPSSALVAQVVGVASSDEDLTGLLWERQQWSAGRFSIFQQHQ*LPDG 6
Score = 39.3 bits (80), Expect(3) = 1e-050
Identities = 17/25 (68%), Positives = 19/25 (76%)
Frame = +2 / +3
Query: 392 SGVLADKYDYAIVVVGEPPYAETFG 466
+ V + KYDYAIVVVGEPP TFG
Sbjct: 264 AAVDSGKYDYAIVVVGEPPDP*TFG 338
Score = 35.2 bits (71), Expect(4) = 2e-048
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / -3
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 274 STAALSGFSEKTTSVLGSTVDLIAERI 194
Score = 35.2 bits (71), Expect(4) = 2e-048
Identities = 16/23 (69%), Positives = 17/23 (73%)
Frame = -3 / -3
Query: 465 PNVSAYGGSPTTTIA*SYLSAST 397
P V GGSPTTT+A*SYL ST
Sbjct: 337 PKV*GSGGSPTTTMA*SYLPLST 269
>gb|BQ469930.1|BQ469930 HX01E12T HX Hordeum vulgare subsp. vulgare cDNA clone HX01E12
5-PRIME, mRNA sequence
Length = 462
Score = 128 bits (276), Expect(2) = 4e-049
Identities = 51/72 (70%), Positives = 61/72 (84%)
Frame = +2 / +1
Query: 179 SSYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAGTTILSGIEATVDP 358
S+ APLLPLPKKA KILVAGSHA++LG QCGGWTI WQG +G TT GTTIL ++A VDP
Sbjct: 28 STDAPLLPLPKKAPKILVAGSHADNLGYQCGGWTIEWQGDTGRTTVGTTILEAVKAAVDP 207
Query: 359 STQVVYSESPDS 394
ST VV++E+PD+
Sbjct: 208 STVVVFAENPDA 243
Score = 87.2 bits (185), Expect(2) = 4e-049
Identities = 36/55 (65%), Positives = 40/55 (72%)
Frame = +2 / +1
Query: 413 YDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLVVE 577
+ YAIV VGE PY ET GDNLNLTIP PG S Q+VCG +C VLISGR VV+
Sbjct: 265 FSYAIVAVGEHPYTETKGDNLNLTIPEPGLSTVQAVCGGVRCATVLISGRSEVVQ 429
Score = 77.2 bits (163), Expect(2) = 9e-029
Identities = 36/68 (52%), Positives = 39/68 (57%)
Frame = -3 / -2
Query: 390 SGLSEYTTCVLGSTVASIPERIXXXXXXXXXXPCHVIVHPPHWLPRSLAWLPATRILPAF 211
SG S TT V GST A R+ PCH +V PPHW PR AWLPATRIL AF
Sbjct: 239 SGFSANTTTVDGSTAAFTASRMVVPTVVRPVSPCHSMVQPPHW*PRLSAWLPATRILGAF 60
Query: 210 FGRGSNGA 187
G G +GA
Sbjct: 59 LGSGRSGA 36
Score = 68.5 bits (144), Expect(2) = 9e-029
Identities = 31/52 (59%), Positives = 34/52 (65%)
Frame = -3 / -2
Query: 573 TTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA* 418
TTS P M T H PHT L PG+G+VRFRLSP VS YG SPT T+A*
Sbjct: 425 TTSDRPLMSTVAHRTPPHTACTVLRPGSGMVRFRLSPLVSVYGCSPTATMA* 270
>gb|AV913828.1|AV913828 AV913828 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags23h03 5', mRNA sequence
Length = 617
Score = 193 bits (418), Expect = 2e-048
Identities = 81/145 (55%), Positives = 103/145 (71%)
Frame = +2 / +2
Query: 2 PMSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKS 181
P+SRIDDAV RILRVKF G+FE+P+ D +L +G +EHR+LAREAVRKSLVLLKNGK+
Sbjct: 92 PLSRIDDAVERILRVKFISGVFEHPFSDPTLLDVIGCKEHRQLAREAVRKSLVLLKNGKN 271
Query: 182 SYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAGTTILSGIEATVDPS 361
P LPL K A +ILV G+HA+++G QCGGWTI W G SG T GT+IL I+ T+
Sbjct: 272 QKEPFLPLAKNAKRILVTGTHADNIGYQCGGWTIAWHGDSGKITPGTSILEAIQRTLQVE 451
Query: 362 TQVVYSESPDSGVLADKYDYAIVVV 436
T+VVY E P + ++ +VV
Sbjct: 452 TEVVYEECPTEATIEEENFSYAIVV 526
Score = 105 bits (225), Expect(2) = 1e-028
Identities = 52/93 (55%), Positives = 60/93 (64%)
Frame = -3 / -3
Query: 291 CHVIVHPPHWLPRSLAWLPATRILPAFFGRGSNGA*EDLPFFRRTRDFLTASRASSRCSC 112
CH IVHPPH P A +P T IL AF +G NG+ LPFFR T+DF TASRAS RCS
Sbjct: 381 CHAIVHPPH*YPILSACVPVTSILFAFLAKGRNGSF*FLPFFRSTKDFRTASRAS*RCSL 202
Query: 111 FPSSPARLESG*GFSNRPMVNLTRRIL*TASSI 13
P + +R+ S G S P +NLT RI TASSI
Sbjct: 201 QPITSSRVGSENGCSKTPEMNLTLRIRSTASSI 103
Score = 39.8 bits (81), Expect(2) = 1e-028
Identities = 21/33 (63%), Positives = 23/33 (69%)
Frame = -3 / -3
Query: 498 PGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAS 400
P GI+R LSPN SA G SPTTTIA* S+S
Sbjct: 591 PLNGILRSVLSPNPSA*GTSPTTTIA*EKFSSS 493
>gb|BG416639.1|BG416639 HVSMEk0013O04f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0013O04f, mRNA sequence
Length = 694
Score = 106 bits (227), Expect(3) = 2e-048
Identities = 43/64 (67%), Positives = 51/64 (79%)
Frame = +2 / +1
Query: 140 AVRKSLVLLKNGKSSYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAG 319
A R SLVLLKNGK++ PLLPLPKKA KIL+AGSHA+DLGNQCGGWTITW+ +G+
Sbjct: 1 ADRMSLVLLKNGKAASTPLLPLPKKAAKILIAGSHADDLGNQCGGWTITWR*QTGHHKTA 180
Query: 320 TTIL 331
T +
Sbjct: 181 GTTI 192
Score = 87.7 bits (186), Expect(3) = 9e-038
Identities = 33/49 (67%), Positives = 37/49 (75%)
Frame = -3 / -3
Query: 288 HVIVHPPHWLPRSLAWLPATRILPAFFGRGSNGA*EDLPFFRRTRDFLT 142
HV+VHPPHWLP+S AWLPA RIL AFFGRG+NG PFF T D L+
Sbjct: 149 HVMVHPPHWLPKSSAWLPAMRILAAFFGRGNNGVEAAFPFFSSTNDILS 3
Score = 84.5 bits (179), Expect(3) = 2e-048
Identities = 38/55 (69%), Positives = 41/55 (74%)
Frame = +2 / +1
Query: 413 YDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLVVE 577
YDYAIVVVG PP +ETF LNLTIPAP PSV +VC C+VVLISG LVVE
Sbjct: 280 YDYAIVVVGHPPNSETFCYYLNLTIPAPRPSVIHNVC*SVTCLVVLISGMPLVVE 444
Score = 82.2 bits (174), Expect(3) = 9e-038
Identities = 37/60 (61%), Positives = 43/60 (71%)
Frame = -3 / -3
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STT+ +PEM TT H+ HT W T G GAGIVRFR NVS +GG PTTT+A*SY+ ST
Sbjct: 443 STTNGMPEMSTTKHVTL*HTLWITEGRGAGIVRFR**QNVSEFGGWPTTTMA*SYMPLST 264
Score = 41.2 bits (84), Expect(3) = 2e-048
Identities = 17/31 (54%), Positives = 24/31 (77%)
Frame = +2 / +1
Query: 311 TAGTTILSGIEATVDPSTQVVYSESPDSGVL 403
TAGTTIL I+ TV P+T+VV+ ++PDS +
Sbjct: 175 TAGTTILWAIKCTVYPNTEVVF*QNPDSSTV 267
Score = 23.8 bits (46), Expect(3) = 9e-038
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = -3 / -3
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG TT VLG TV I +RI
Sbjct: 269 STVELSGFC*KTTSVLG*TVHLIAQRI 189
>gb|BM098044.2|BM098044 EBpi03_SQ002_G06_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ002_G06 5', mRNA sequence
Length = 471
Score = 95.9 bits (204), Expect(3) = 7e-048
Identities = 35/53 (66%), Positives = 44/53 (83%)
Frame = +2 / +1
Query: 236 GSHANDLGNQCGGWTITWQGSSGNTTAGTTILSGIEATVDPSTQVVYSESPDS 394
GSHA++LG QCGGWTI WQG +G TT GTTIL ++A VDPST VV++E+PD+
Sbjct: 163 GSHADNLGYQCGGWTIEWQGDTGRTTVGTTILEAVKAAVDPSTVVVFAENPDA 321
Score = 71.7 bits (151), Expect(3) = 7e-048
Identities = 28/42 (66%), Positives = 31/42 (73%)
Frame = +2 / +1
Query: 413 YDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKC 538
+ YAIV VGE PY ET GDNLNLTIP PG S Q+VCG +C
Sbjct: 343 FSYAIVAVGEHPYTETKGDNLNLTIPEPGLSTVQAVCGGVRC 468
Score = 62.6 bits (131), Expect(3) = 7e-048
Identities = 26/34 (76%), Positives = 31/34 (91%)
Frame = +2 / +2
Query: 83 DSSLAGELGKQEHRELAREAVRKSLVLLKNGKSS 184
D ++A +LGKQEHR+LAREA RKSLVLLKNGK+S
Sbjct: 5 DPAMAEQLGKQEHRDLAREAARKSLVLLKNGKTS 106
Score = 57.6 bits (120), Expect(3) = 2e-027
Identities = 25/40 (62%), Positives = 28/40 (70%)
Frame = -3 / -2
Query: 537 HLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA* 418
H PHT L PG+G+VRFRLSP VS YG SPT T+A*
Sbjct: 467 HRTPPHTACTVLRPGSGMVRFRLSPLVSVYGCSPTATMA* 348
Score = 53.9 bits (112), Expect(3) = 2e-027
Identities = 25/52 (48%), Positives = 27/52 (51%)
Frame = -3 / -2
Query: 390 SGLSEYTTCVLGSTVASIPERIXXXXXXXXXXPCHVIVHPPHWLPRSLAWLP 235
SG S TT V GST A R+ PCH +V PPHW PR AWLP
Sbjct: 317 SGFSANTTTVDGSTAAFTASRMVVPTVVRPVSPCHSMVQPPHW*PRLSAWLP 162
Score = 47.1 bits (97), Expect(3) = 2e-027
Identities = 21/35 (60%), Positives = 22/35 (62%)
Frame = -3 / -1
Query: 198 SNGA*EDLPFFRRTRDFLTASRASSRCSCFPSSPA 94
S + E LPFF T DFL AS A S CSCFPS A
Sbjct: 120 SGASVEVLPFFSSTSDFLAASLAKSLCSCFPSCSA 16
>gb|CA032659.1|CA032659 HX13N01r HX Hordeum vulgare subsp. vulgare cDNA clone HX13N01
5-PRIME, mRNA sequence
Length = 326
Score = 189 bits (410), Expect = 2e-047
Identities = 77/93 (82%), Positives = 83/93 (89%)
Frame = +2 / +1
Query: 53 TMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKSSYAPLLPLPKKAGKILV 232
TMGLFE+PY D SLAGELGKQEHR+LAREAVRKSLVLLKNGKS+Y PLLPLPKKAGKILV
Sbjct: 19 TMGLFESPYADPSLAGELGKQEHRDLAREAVRKSLVLLKNGKSAYTPLLPLPKKAGKILV 198
Query: 233 AGSHANDLGNQCGGWTITWQGSSGNTTAGTTIL 331
AGSHA+DLGNQCGGWTITWQG +GN T +
Sbjct: 199 AGSHADDLGNQCGGWTITWQGQTGNDKTAGTTI 297
Score = 167 bits (361), Expect(2) = 1e-043
Identities = 66/80 (82%), Positives = 69/80 (86%)
Frame = -3 / -1
Query: 291 CHVIVHPPHWLPRSLAWLPATRILPAFFGRGSNGA*EDLPFFRRTRDFLTASRASSRCSC 112
CHV+VHPPHWLP+S AWLPATRILPAFFGRG+NG * D PFF T DFLTASRA SRCSC
Sbjct: 257 CHVMVHPPHWLPKSSAWLPATRILPAFFGRGNNGV*ADFPFFSNTNDFLTASRARSRCSC 78
Query: 111 FPSSPARLESG*GFSNRPMV 52
FPSSPARL S *G SNRPMV
Sbjct: 77 FPSSPARLGSA*GLSNRPMV 18
Score = 27.0 bits (53), Expect(2) = 1e-043
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = -2 / -3
Query: 355 VHGGLDPGEDRRAGSSVAA 299
V GGLD +DRR GS V A
Sbjct: 324 VDGGLDRRKDRRPGSFVVA 268
>gb|AL503553.1|AL503553 AL503553 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW02J07T 5', mRNA sequence
Length = 700
Score = 152 bits (328), Expect(3) = 3e-044
Identities = 65/74 (87%), Positives = 69/74 (93%)
Frame = +2 / +3
Query: 2 PMSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKS 181
PMSRI+DAVYRILRVKFTMGLFE+PY D SL GELGKQEHR+LAREAVRKSLVLLKNGKS
Sbjct: 384 PMSRINDAVYRILRVKFTMGLFESPYADPSLVGELGKQEHRDLAREAVRKSLVLLKNGKS 563
Query: 182 SYAPLLPLPKKAGK 223
+ PLLPLPKKAGK
Sbjct: 564 ASTPLLPLPKKAGK 605
Score = 131 bits (283), Expect(2) = 1e-037
Identities = 58/74 (78%), Positives = 59/74 (79%)
Frame = -3 / -1
Query: 222 LPAFFGRGSNGA*EDLPFFRRTRDFLTASRASSRCSCFPSSPARLESG*GFSNRPMVNLT 43
LPAFFGRG+NG D PFF T DFLTASRA SRCSCFPSSP RL S *G SNRPMVN T
Sbjct: 604 LPAFFGRGNNGVEADFPFFSNTNDFLTASRARSRCSCFPSSPTRLGSA*GLSNRPMVNFT 425
Query: 42 RRIL*TASSILLMG 1
RIL*TAS ILLMG
Sbjct: 424 LRIL*TASLILLMG 383
Score = 43.4 bits (89), Expect(2) = 1e-037
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = -3 / -3
Query: 270 PHWLPRSLAWLPATRIL 220
PHWLP+S AWLPATRIL
Sbjct: 653 PHWLPKSSAWLPATRIL 603
Score = 40.7 bits (83), Expect(3) = 3e-044
Identities = 15/17 (88%), Positives = 17/17 (100%)
Frame = +2 / +1
Query: 221 KILVAGSHANDLGNQCG 271
+ILVAGSHA+DLGNQCG
Sbjct: 604 RILVAGSHADDLGNQCG 654
Score = 24.7 bits (48), Expect(3) = 3e-044
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = +2 / +2
Query: 269 GGWTITW 289
GGWTITW
Sbjct: 653 GGWTITW 673
>gb|AV913362.1|AV913362 AV913362 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags21m18 5', mRNA sequence
Length = 585
Score = 119 bits (255), Expect(2) = 6e-043
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +2 / +1
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 193 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLV 372
Query: 572 VE 577
VE
Sbjct: 373 VE 378
Score = 112 bits (241), Expect(3) = 1e-043
Identities = 49/60 (81%), Positives = 50/60 (83%)
Frame = -3 / -2
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 377 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 198
Score = 75.8 bits (160), Expect(2) = 6e-043
Identities = 28/38 (73%), Positives = 31/38 (81%)
Frame = +2 / +1
Query: 218 GKILVAGSHANDLGNQCGGWTITWQGSSGNTTAGTTIL 331
GKILVAGSHA+DLGNQCGGWTITWQG +GN T +
Sbjct: 13 GKILVAGSHADDLGNQCGGWTITWQGQTGNDKTAGTTI 126
Score = 65.8 bits (138), Expect(3) = 1e-043
Identities = 22/26 (84%), Positives = 25/26 (96%)
Frame = -3 / -2
Query: 291 CHVIVHPPHWLPRSLAWLPATRILPA 214
CHV+VHPPHWLP+S AWLPATRILP+
Sbjct: 86 CHVMVHPPHWLPKSSAWLPATRILPS 9
Score = 35.2 bits (71), Expect(3) = 1e-043
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / -2
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 203 STAALSGFSEKTTSVLGSTVDLIAERI 123
>gb|AV918661.1|AV918661 AV918661 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags21m18 3', mRNA sequence
Length = 663
Score = 119 bits (255), Expect(2) = 6e-043
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +2 / -1
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 483 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLV 304
Query: 572 VE 577
VE
Sbjct: 303 VE 298
Score = 112 bits (241), Expect(3) = 1e-042
Identities = 49/60 (81%), Positives = 50/60 (83%)
Frame = -3 / +2
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 299 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 478
Score = 75.8 bits (160), Expect(2) = 6e-043
Identities = 28/38 (73%), Positives = 31/38 (81%)
Frame = +2 / -1
Query: 218 GKILVAGSHANDLGNQCGGWTITWQGSSGNTTAGTTIL 331
GKILVAGSHA+DLGNQCGGWTITWQG +GN T +
Sbjct: 663 GKILVAGSHADDLGNQCGGWTITWQGQTGNDKTAGTTI 550
Score = 62.1 bits (130), Expect(3) = 1e-042
Identities = 21/24 (87%), Positives = 23/24 (95%)
Frame = -3 / +2
Query: 291 CHVIVHPPHWLPRSLAWLPATRIL 220
CHV+VHPPHWLP+S AWLPATRIL
Sbjct: 590 CHVMVHPPHWLPKSSAWLPATRIL 661
Score = 35.2 bits (71), Expect(3) = 1e-042
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / +2
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 473 STAALSGFSEKTTSVLGSTVDLIAERI 553
>gb|AV920100.1|AV920100 AV920100 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags9e08 3', mRNA sequence
Length = 682
Score = 119 bits (255), Expect(2) = 6e-037
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +2 / -1
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 511 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLV 332
Query: 572 VE 577
VE
Sbjct: 331 VE 326
Score = 112 bits (241), Expect(3) = 2e-036
Identities = 49/60 (81%), Positives = 50/60 (83%)
Frame = -3 / +3
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 327 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 506
Score = 55.8 bits (116), Expect(2) = 6e-037
Identities = 22/36 (61%), Positives = 30/36 (83%)
Frame = +2 / -1
Query: 296 SSGNTTAGTTILSGIEATVDPSTQVVYSESPDSGVL 403
+ + TAGTTILS I++TVDPST+VV+SE+PDS +
Sbjct: 610 TGNDKTAGTTILSAIKSTVDPSTEVVFSENPDSAAV 503
Score = 41.2 bits (84), Expect(3) = 2e-036
Identities = 12/14 (85%), Positives = 14/14 (100%)
Frame = -3 / +3
Query: 291 CHVIVHPPHWLPRS 250
CHV+VHPPHWLP+S
Sbjct: 618 CHVMVHPPHWLPKS 659
Score = 35.2 bits (71), Expect(3) = 2e-036
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / +3
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 501 STAALSGFSEKTTSVLGSTVDLIAERI 581
>gb|AV930613.1|AV930613 AV930613 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd24f16 3', mRNA sequence
Length = 599
Score = 119 bits (255), Expect(2) = 6e-037
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +2 / -1
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 476 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLV 297
Query: 572 VE 577
VE
Sbjct: 296 VE 291
Score = 112 bits (241), Expect(2) = 2e-029
Identities = 49/60 (81%), Positives = 50/60 (83%)
Frame = -3 / +1
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 292 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 471
Score = 55.8 bits (116), Expect(2) = 6e-037
Identities = 22/36 (61%), Positives = 30/36 (83%)
Frame = +2 / -1
Query: 296 SSGNTTAGTTILSGIEATVDPSTQVVYSESPDSGVL 403
+ + TAGTTILS I++TVDPST+VV+SE+PDS +
Sbjct: 575 TGNDKTAGTTILSAIKSTVDPSTEVVFSENPDSAAV 468
Score = 35.2 bits (71), Expect(2) = 2e-029
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / +1
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 466 STAALSGFSEKTTSVLGSTVDLIAERI 546
>gb|BQ765660.1|BQ765660 EBro03_SQ007_F19_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ007_F19 5', mRNA sequence
Length = 371
Score = 119 bits (255), Expect(2) = 6e-037
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +2 / +3
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 99 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLV 278
Query: 572 VE 577
VE
Sbjct: 279 VE 284
Score = 112 bits (241), Expect(2) = 1e-029
Identities = 49/60 (81%), Positives = 50/60 (83%)
Frame = -3 / -2
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 283 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 104
Score = 55.3 bits (115), Expect(2) = 6e-037
Identities = 22/31 (70%), Positives = 28/31 (90%)
Frame = +2 / +3
Query: 311 TAGTTILSGIEATVDPSTQVVYSESPDSGVL 403
TAGTTILS I++TVDPST+VV+SE+PDS +
Sbjct: 15 TAGTTILSAIKSTVDPSTEVVFSENPDSAAV 107
Score = 35.2 bits (71), Expect(2) = 1e-029
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / -2
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 109 STAALSGFSEKTTSVLGSTVDLIAERI 29
>gb|AV911128.1|AV911128 AV911128 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak12b24 3', mRNA sequence
Length = 609
Score = 117 bits (252), Expect(2) = 2e-036
Identities = 49/62 (79%), Positives = 54/62 (87%)
Frame = +2 / -3
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 514 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQTVCKSVRCVVVLISGRPLV 335
Query: 572 VE 577
+E
Sbjct: 334 LE 329
Score = 110 bits (237), Expect(2) = 6e-029
Identities = 48/60 (80%), Positives = 50/60 (83%)
Frame = -3 / +3
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
S+TS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 330 SSTSGLPEMSTTTHLTLLHTVWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 509
Score = 55.3 bits (115), Expect(2) = 2e-036
Identities = 22/31 (70%), Positives = 28/31 (90%)
Frame = +2 / -3
Query: 311 TAGTTILSGIEATVDPSTQVVYSESPDSGVL 403
TAGTTILS I++TVDPST+VV+SE+PDS +
Sbjct: 598 TAGTTILSAIKSTVDPSTEVVFSENPDSAAV 506
Score = 35.2 bits (71), Expect(2) = 6e-029
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / +3
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 504 STAALSGFSEKTTSVLGSTVDLIAERI 584
>gb|BF627275.3|BF627275 HVSMEb0004G19f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0004G19f, mRNA sequence
Length = 636
Score = 119 bits (255), Expect(2) = 4e-036
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +2 / +3
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 90 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLV 269
Query: 572 VE 577
VE
Sbjct: 270 VE 275
Score = 112 bits (241), Expect(2) = 2e-029
Identities = 49/60 (81%), Positives = 50/60 (83%)
Frame = -3 / -3
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 274 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 95
Score = 53.0 bits (110), Expect(2) = 4e-036
Identities = 21/30 (70%), Positives = 27/30 (90%)
Frame = +2 / +3
Query: 314 AGTTILSGIEATVDPSTQVVYSESPDSGVL 403
AGTTILS I++TVDPST+VV+SE+PDS +
Sbjct: 9 AGTTILSAIKSTVDPSTEVVFSENPDSAAV 98
Score = 35.2 bits (71), Expect(2) = 2e-029
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / -3
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 100 STAALSGFSEKTTSVLGSTVDLIAERI 20
>gb|AV909243.1|AV909243 AV909243 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak12b24 5', mRNA sequence
Length = 655
Score = 117 bits (252), Expect(2) = 3e-035
Identities = 49/62 (79%), Positives = 54/62 (87%)
Frame = +2 / +1
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 121 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQTVCKSVRCVVVLISGRPLV 300
Query: 572 VE 577
+E
Sbjct: 301 LE 306
Score = 110 bits (237), Expect(2) = 6e-029
Identities = 48/60 (80%), Positives = 50/60 (83%)
Frame = -3 / -3
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
S+TS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 305 SSTSGLPEMSTTTHLTLLHTVWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 126
Score = 51.2 bits (106), Expect(2) = 3e-035
Identities = 20/29 (68%), Positives = 26/29 (89%)
Frame = +2 / +1
Query: 317 GTTILSGIEATVDPSTQVVYSESPDSGVL 403
GTTILS I++TVDPST+VV+SE+PDS +
Sbjct: 43 GTTILSAIKSTVDPSTEVVFSENPDSAAV 129
Score = 35.2 bits (71), Expect(2) = 6e-029
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / -3
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 131 STAALSGFSEKTTSVLGSTVDLIAERI 51
>gb|BQ761635.1|BQ761635 EBem10_SQ001_J09_R embryo, 2 Day germination, no treatment, cv
Optic, EBem10 Hordeum vulgare subsp. vulgare cDNA clone
EBem10_SQ001_J09 5', mRNA sequence
Length = 255
Score = 116 bits (250), Expect(2) = 4e-033
Identities = 49/61 (80%), Positives = 53/61 (86%)
Frame = +2 / +3
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 72 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLV 251
Query: 572 V 574
V
Sbjct: 252 V 254
Score = 110 bits (237), Expect(2) = 3e-029
Identities = 48/59 (81%), Positives = 49/59 (83%)
Frame = -3 / -3
Query: 573 TTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
TTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 253 TTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 77
Score = 43.9 bits (90), Expect(2) = 4e-033
Identities = 17/26 (65%), Positives = 23/26 (88%)
Frame = +2 / +3
Query: 326 ILSGIEATVDPSTQVVYSESPDSGVL 403
ILS I++TVDPST+VV+SE+PDS +
Sbjct: 3 ILSAIKSTVDPSTEVVFSENPDSAAV 80
Score = 35.2 bits (71), Expect(2) = 3e-029
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = -3 / -3
Query: 405 ASTPLSGLSEYTTCVLGSTVASIPERI 325
++ LSG SE TT VLGSTV I ERI
Sbjct: 82 STAALSGFSEKTTSVLGSTVDLIAERI 2
>gb|BI951410.1|BI951410 HVSMEl0025N13f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0025N13f, mRNA sequence
Length = 581
Score = 120 bits (259), Expect(2) = 1e-028
Identities = 50/85 (58%), Positives = 63/85 (74%)
Frame = +2 / +3
Query: 140 AVRKSLVLLKNGKSSYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAG 319
A + ++L S+ APLLPLPKKA KILVAGSHA++LG QCGGWTI WQG +G TT G
Sbjct: 132 ARKSLVLLKNGKTSTDAPLLPLPKKAPKILVAGSHADNLGYQCGGWTIEWQGDTGRTTVG 311
Query: 320 TTILSGIEATVDPSTQVVYSESPDS 394
TTIL ++A VDP T V++ +PD+
Sbjct: 312 TTILEAVKAAVDPFTDEVFT*NPDA 386
Score = 110 bits (236), Expect = 1e-023
Identities = 47/57 (82%), Positives = 52/57 (91%)
Frame = +2 / +3
Query: 14 IDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKSS 184
IDDAV RILRVKFTMGLFENPY D ++A +LGKQEHR+LAREA RKSLVLLKNGK+S
Sbjct: 3 IDDAVTRILRVKFTMGLFENPYADPAMAEQLGKQEHRDLAREAARKSLVLLKNGKTS 173
Score = 84.5 bits (179), Expect(2) = 2e-031
Identities = 41/62 (66%), Positives = 42/62 (67%)
Frame = -3 / -2
Query: 198 SNGA*EDLPFFRRTRDFLTASRASSRCSCFPSSPARLESG*GFSNRPMVNLTRRIL*TAS 19
S + E LPFF T DFL AS A S CSCFPS A S *GFS RPMVNLTRRI TAS
Sbjct: 187 SGASVEVLPFFSSTSDFLAASLAKSLCSCFPSCSAMAGSA*GFSKRPMVNLTRRIRVTAS 8
Query: 18 SI 13
SI
Sbjct: 7 SI 2
Score = 69.9 bits (147), Expect(2) = 2e-031
Identities = 33/68 (48%), Positives = 37/68 (54%)
Frame = -3 / -2
Query: 390 SGLSEYTTCVLGSTVASIPERIXXXXXXXXXXPCHVIVHPPHWLPRSLAWLPATRILPAF 211
SG T+ V GST A + PCH +V PPHW PR AWLPATRIL AF
Sbjct: 382 SGF*VNTSSVKGSTAAFTASNMVVPTVVRPVSPCHSMVQPPHW*PRLSAWLPATRILGAF 203
Query: 210 FGRGSNGA 187
G G +GA
Sbjct: 202 LGSGRSGA 179
Score = 25.7 bits (50), Expect(2) = 1e-028
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +1 / +2
Query: 13 NRRRRLQDPSCQVHHG 60
+RR R DP+ QVHHG
Sbjct: 2 DRRCRDPDPAGQVHHG 49
>gb|AV917377.1|AV917377 AV917377 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags18k19 5', mRNA sequence
Length = 192
Score = 135 bits (290), Expect = 5e-031
Identities = 57/64 (89%), Positives = 60/64 (93%)
Frame = +2 / +1
Query: 83 DSSLAGELGKQEHRELAREAVRKSLVLLKNGKSSYAPLLPLPKKAGKILVAGSHANDLGN 262
D SL GELGKQEHR+LAREAVRKSLVLLKNGKS+ PLLPLPKKAGKILVAGSHA+DLGN
Sbjct: 1 DPSLVGELGKQEHRDLAREAVRKSLVLLKNGKSASTPLLPLPKKAGKILVAGSHADDLGN 180
Query: 263 QCGG 274
QCGG
Sbjct: 181 QCGG 192
Score = 129 bits (278), Expect = 8e-030
Identities = 50/62 (80%), Positives = 52/62 (83%)
Frame = -3 / -2
Query: 273 PPHWLPRSLAWLPATRILPAFFGRGSNGA*EDLPFFRRTRDFLTASRASSRCSCFPSSPA 94
PPHWLP+S AWLPATRILPAFFGRG+NG D PFF T DFLTASRA SRCSCFPSSP
Sbjct: 191 PPHWLPKSSAWLPATRILPAFFGRGNNGVEADFPFFSNTNDFLTASRARSRCSCFPSSPT 12
Query: 93 RL 88
RL
Sbjct: 11 RL 6
>gb|BU997464.1|BU997464 HI08B05r HI Hordeum vulgare subsp. vulgare cDNA clone HI08B05
5-PRIME, mRNA sequence
Length = 594
Score = 119 bits (255), Expect(2) = 2e-030
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +2 / -1
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 549 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLV 370
Query: 572 VE 577
VE
Sbjct: 369 VE 364
Score = 112 bits (241), Expect(2) = 2e-026
Identities = 49/60 (81%), Positives = 50/60 (83%)
Frame = -3 / +2
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 365 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 544
Score = 33.4 bits (67), Expect(2) = 2e-030
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = +2 / -1
Query: 350 VDPSTQVVYSESPDSGVL 403
VDPST+VV+SE+PDS +
Sbjct: 594 VDPSTEVVFSENPDSAAV 541
Score = 25.2 bits (49), Expect(2) = 2e-026
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = -3 / +2
Query: 405 ASTPLSGLSEYTTCVLGS 352
++ LSG SE TT VLGS
Sbjct: 539 STAALSGFSEKTTSVLGS 592
>gb|BU995937.1|BU995937 HM11N11r HM Hordeum vulgare subsp. vulgare cDNA clone HM11N11
5-PRIME, mRNA sequence
Length = 636
Score = 103 bits (220), Expect(3) = 4e-030
Identities = 42/57 (73%), Positives = 46/57 (80%)
Frame = +2 / +3
Query: 407 DKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLVVE 577
D+YDYA+VVVGE PYA T GDNLNLTIP PGP V + VC KCVVVL+SGR LVVE
Sbjct: 183 DEYDYAVVVVGEHPYAATAGDNLNLTIPGPGPEVIRKVCELVKCVVVLVSGRPLVVE 353
Score = 39.8 bits (81), Expect(3) = 4e-030
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = +2 / +3
Query: 263 QCGGWTITWQGSSGNTTAG 319
QCGGWT +WQG SGN G
Sbjct: 24 QCGGWTKSWQGQSGNGITG 80
Score = 26.6 bits (52), Expect(3) = 4e-030
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +1 / +2
Query: 295 KQRQHYCRHDDPLRDRGHRGPQHAGGVLGEPGQ 393
+QR H RH P D+ G + + G PGQ
Sbjct: 62 RQRHHRTRHHHP*GDQVGGGQEDGHRLHGAPGQ 160
>gb|CA022582.1|CA022582 HZ43L07r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ43L07
5-PRIME, mRNA sequence
Length = 416
Score = 129 bits (278), Expect = 2e-029
Identities = 53/85 (62%), Positives = 66/85 (77%)
Frame = +2 / +3
Query: 140 AVRKSLVLLKNGKSSYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAG 319
A + ++L S+ APLLPLPKKA KILVAGSHA++LG QCGGWTI WQG +G TT G
Sbjct: 126 ARKSLVLLKNGKTSTDAPLLPLPKKAPKILVAGSHADNLGYQCGGWTIEWQGDTGRTTVG 305
Query: 320 TTILSGIEATVDPSTQVVYSESPDS 394
TTIL ++A VDPST VV++E+PD+
Sbjct: 306 TTILEAVKAAVDPSTVVVFAENPDA 380
Score = 77.2 bits (163), Expect(2) = 1e-027
Identities = 36/68 (52%), Positives = 39/68 (57%)
Frame = -3 / -2
Query: 390 SGLSEYTTCVLGSTVASIPERIXXXXXXXXXXPCHVIVHPPHWLPRSLAWLPATRILPAF 211
SG S TT V GST A R+ PCH +V PPHW PR AWLPATRIL AF
Sbjct: 376 SGFSANTTTVDGSTAAFTASRMVVPTVVRPVSPCHSMVQPPHW*PRLSAWLPATRILGAF 197
Query: 210 FGRGSNGA 187
G G +GA
Sbjct: 196 LGSGRSGA 173
Score = 64.4 bits (135), Expect(2) = 1e-027
Identities = 30/49 (61%), Positives = 31/49 (63%)
Frame = -3 / -2
Query: 198 SNGA*EDLPFFRRTRDFLTASRASSRCSCFPSSPARLESG*GFSNRPMV 52
S + E LPFF T DFL AS A S CSCFPS A S *GFS RPMV
Sbjct: 181 SGASVEVLPFFSSTSDFLAASLAKSLCSCFPSCSAMAGSA*GFSKRPMV 35
>gb|BM100567.2|BM100567 EBma01_SQ005_J17_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ005_J17 5', mRNA sequence
Length = 275
Score = 129 bits (278), Expect = 2e-029
Identities = 53/85 (62%), Positives = 66/85 (77%)
Frame = +2 / +3
Query: 140 AVRKSLVLLKNGKSSYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAG 319
A + ++L S+ APLLPLPKKA KILVAGSHA++LG QCGGWTI WQG +G TT G
Sbjct: 21 ARKSLVLLKNGKTSTDAPLLPLPKKAPKILVAGSHADNLGYQCGGWTIEWQGDTGRTTVG 200
Query: 320 TTILSGIEATVDPSTQVVYSESPDS 394
TTIL ++A VDPST VV++E+PD+
Sbjct: 201 TTILEAVKAAVDPSTVVVFAENPDA 275
>gb|CA015249.1|CA015249 HT13L19r HT Hordeum vulgare subsp. vulgare cDNA clone HT13L19
5-PRIME, mRNA sequence
Length = 570
Score = 88.1 bits (187), Expect(2) = 3e-029
Identities = 36/55 (65%), Positives = 41/55 (74%)
Frame = +2 / +2
Query: 413 YDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLVVE 577
+ YAIV VGE PY ET GDNLNLTIP PG S Q+VCG +C VLISGR +VV+
Sbjct: 134 FSYAIVAVGEHPYTETKGDNLNLTIPEPGLSTVQAVCGGVRCATVLISGRPVVVQ 298
Score = 60.8 bits (127), Expect(2) = 3e-029
Identities = 22/36 (61%), Positives = 29/36 (80%)
Frame = +2 / +2
Query: 287 WQGSSGNTTAGTTILSGIEATVDPSTQVVYSESPDS 394
WQG +G TT GTTIL ++A VDPST VV++E+PD+
Sbjct: 5 WQGDTGRTTVGTTILEAVKAAVDPSTVVVFAENPDA 112
>gb|BQ468423.1|BQ468423 HM01D10T HM Hordeum vulgare subsp. vulgare cDNA clone HM01D10
5-PRIME, mRNA sequence
Length = 634
Score = 109 bits (234), Expect(2) = 2e-028
Identities = 46/58 (79%), Positives = 52/58 (89%)
Frame = +2 / +1
Query: 5 MSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGK 178
M RI+DAV RILRVKF+MGLFENP+PD +L G+LG + HRELAREAVRKSLVLLKNGK
Sbjct: 385 MDRINDAVTRILRVKFSMGLFENPFPDKTLTGQLGAKAHRELAREAVRKSLVLLKNGK 558
Score = 36.6 bits (74), Expect(2) = 2e-028
Identities = 15/25 (60%), Positives = 18/25 (72%)
Frame = +2 / +1
Query: 173 GKSSYAPLLPLPKKAGKILVAGSHA 247
G+ P+LPL KKA KILV G+HA
Sbjct: 559 GRKDGKPVLPLSKKAKKILVVGTHA 633
>gb|CB881387.1|CB881387 HM09K13w HM Hordeum vulgare subsp. vulgare cDNA clone HM09K13
3-PRIME, mRNA sequence
Length = 638
Score = 106 bits (227), Expect(2) = 8e-028
Identities = 43/57 (75%), Positives = 47/57 (82%)
Frame = +2 / -2
Query: 407 DKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLVVE 577
D+YDYA+VVVGE PYAET GDNLNLTIP PGP V + VC KCVVVL+SGR LVVE
Sbjct: 523 DEYDYAVVVVGEQPYAETAGDNLNLTIPGPGPEVIRKVCELVKCVVVLVSGRPLVVE 353
Score = 37.5 bits (76), Expect(2) = 8e-028
Identities = 16/37 (43%), Positives = 21/37 (56%)
Frame = +2 / -2
Query: 308 TTAGTTILSGIEATVDPSTQVVYSESPDSGVLADKYD 418
T GTTI I++ VD T + YSE PD G ++ D
Sbjct: 631 TGQGTTIFEAIKSAVDKKTVIDYSEHPDKGNVSKSED 521
>gb|BQ663930.1|BQ663930 HU04K15u HU Hordeum vulgare subsp. vulgare cDNA clone HU04K15
3-PRIME, mRNA sequence
Length = 582
Score = 119 bits (255), Expect(2) = 1e-027
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +2 / -1
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 525 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLV 346
Query: 572 VE 577
VE
Sbjct: 345 VE 340
Score = 112 bits (241), Expect(2) = 5e-027
Identities = 49/60 (81%), Positives = 50/60 (83%)
Frame = -3 / +2
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 341 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 520
Score = 27.0 bits (53), Expect(2) = 5e-027
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = -2 / +3
Query: 373 HHLRAGVHGGLD 338
+HLRAGV GGLD
Sbjct: 546 NHLRAGVDGGLD 581
Score = 24.3 bits (47), Expect(2) = 1e-027
Identities = 9/12 (75%), Positives = 12/12 (100%)
Frame = +2 / -2
Query: 338 IEATVDPSTQVV 373
I++TVDPST+VV
Sbjct: 581 IKSTVDPSTEVV 546
>gb|BQ464740.1|BQ464740 HU01E02T HU Hordeum vulgare subsp. vulgare cDNA clone HU01E02
5-PRIME, mRNA sequence
Length = 563
Score = 120 bits (257), Expect = 1e-026
Identities = 51/61 (83%), Positives = 56/61 (91%)
Frame = +2 / +1
Query: 2 PMSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKS 181
PMSRIDDAV RILRVKFTMGLFENPY D ++A +LGKQEHR+LAREA RKSLVLLKNGK+
Sbjct: 346 PMSRIDDAVTRILRVKFTMGLFENPYADPAMAEQLGKQEHRDLAREAARKSLVLLKNGKT 525
Query: 182 S 184
S
Sbjct: 526 S 528
>gb|CA020043.1|CA020043 HV14C18r HV Hordeum vulgare subsp. vulgare cDNA clone HV14C18
5-PRIME, mRNA sequence
Length = 542
Score = 120 bits (257), Expect = 1e-026
Identities = 51/61 (83%), Positives = 56/61 (91%)
Frame = +2 / +2
Query: 2 PMSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKS 181
PMSRIDDAV RILRVKFTMGLFENPY D ++A +LGKQEHR+LAREA RKSLVLLKNGK+
Sbjct: 290 PMSRIDDAVTRILRVKFTMGLFENPYADPAMAEQLGKQEHRDLAREAARKSLVLLKNGKT 469
Query: 182 S 184
S
Sbjct: 470 S 472
>gb|BJ467252.1|BJ467252 BJ467252 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags26e21 3', mRNA sequence
Length = 522
Score = 119 bits (255), Expect = 3e-026
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +2 / -3
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 505 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLV 326
Query: 572 VE 577
VE
Sbjct: 325 VE 320
Score = 112 bits (241), Expect = 1e-024
Identities = 49/60 (81%), Positives = 50/60 (83%)
Frame = -3 / +3
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 321 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 500
>gb|CB858339.1|CB858339 HI06K15w HI Hordeum vulgare subsp. vulgare cDNA clone HI06K15
3-PRIME, mRNA sequence
Length = 542
Score = 119 bits (255), Expect = 3e-026
Identities = 50/62 (80%), Positives = 54/62 (87%)
Frame = +2 / -3
Query: 392 SGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLV 571
+ V + KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LV
Sbjct: 525 AAVDSGKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLV 346
Query: 572 VE 577
VE
Sbjct: 345 VE 340
Score = 112 bits (241), Expect = 1e-024
Identities = 49/60 (81%), Positives = 50/60 (83%)
Frame = -3 / +2
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 341 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 520
>gb|BM374002.2|BM374002 EBma03_SQ003_F17_R maternal, 8 DPA, no treatment, cv Optic, EBma03
Hordeum vulgare subsp. vulgare cDNA clone
EBma03_SQ003_F17 5', mRNA sequence
Length = 533
Score = 118 bits (254), Expect = 4e-026
Identities = 49/56 (87%), Positives = 51/56 (91%)
Frame = +2 / +2
Query: 410 KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLVVE 577
KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LVVE
Sbjct: 17 KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLVVE 184
Score = 112 bits (241), Expect = 1e-024
Identities = 49/60 (81%), Positives = 50/60 (83%)
Frame = -3 / -3
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYLSAST 397
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL ST
Sbjct: 183 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYLPLST 4
>gb|BM098018.2|BM098018 EBpi03_SQ002_F03_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ002_F03 5', mRNA sequence
Length = 523
Score = 118 bits (254), Expect = 4e-026
Identities = 49/56 (87%), Positives = 51/56 (91%)
Frame = +2 / +3
Query: 410 KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLVVE 577
KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSV Q+VC +CVVVLISGR LVVE
Sbjct: 18 KYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVIQNVCKSVRCVVVLISGRPLVVE 185
Score = 109 bits (234), Expect = 9e-024
Identities = 47/56 (83%), Positives = 48/56 (85%)
Frame = -3 / -1
Query: 576 STTSXLPEMRTTTHLAXPHTDWXTLGPGAGIVRFRLSPNVSAYGGSPTTTIA*SYL 409
STTS LPEM TTTHL HT W T GPGAGIVRFRLSPNVSAYGGSPTTT+A*SYL
Sbjct: 184 STTSGLPEMSTTTHLTLLHTFWITEGPGAGIVRFRLSPNVSAYGGSPTTTMA*SYL 17
>gb|BQ462564.1|BQ462564 HI01E18T HI Hordeum vulgare subsp. vulgare cDNA clone HI01E18
5-PRIME, mRNA sequence
Length = 596
Score = 117 bits (251), Expect = 1e-025
Identities = 50/61 (81%), Positives = 55/61 (90%)
Frame = +2 / +2
Query: 2 PMSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKS 181
PMSRIDDAV RILRVKFTMGL ENPY D ++A +LGKQEHR+LAREA RKSLVLLKNGK+
Sbjct: 350 PMSRIDDAVTRILRVKFTMGLLENPYADPAMAEQLGKQEHRDLAREAARKSLVLLKNGKT 529
Query: 182 S 184
S
Sbjct: 530 S 532
Score = 93.1 bits (198), Expect(2) = 7e-024
Identities = 45/66 (68%), Positives = 46/66 (69%)
Frame = -3 / -3
Query: 198 SNGA*EDLPFFRRTRDFLTASRASSRCSCFPSSPARLESG*GFSNRPMVNLTRRIL*TAS 19
S + E LPFF T DFL AS A S CSCFPS A S *GFS RPMVNLTRRI TAS
Sbjct: 546 SGASVEVLPFFSSTSDFLAASLAKSLCSCFPSCSAMAGSA*GFSKRPMVNLTRRIRVTAS 367
Query: 18 SILLMG 1
SILLMG
Sbjct: 366 SILLMG 349
Score = 36.1 bits (73), Expect(2) = 7e-024
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -3 / -3
Query: 243 WLPATRILPAFFGRGSNGA 187
WLPATRIL AF G G +GA
Sbjct: 594 WLPATRILGAFLGSGRSGA 538
>gb|BQ462668.1|BQ462668 HI01K09T HI Hordeum vulgare subsp. vulgare cDNA clone HI01K09
5-PRIME, mRNA sequence
Length = 551
Score = 117 bits (251), Expect = 1e-025
Identities = 50/61 (81%), Positives = 55/61 (90%)
Frame = +2 / +3
Query: 2 PMSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKS 181
PMSRIDDAV RILRVKFTMGLFENPY D ++A +LGKQEHR+LAREA RKSLVLLKNG +
Sbjct: 351 PMSRIDDAVTRILRVKFTMGLFENPYADPAMAEQLGKQEHRDLAREAARKSLVLLKNGMT 530
Query: 182 S 184
S
Sbjct: 531 S 533
>gb|BQ468807.1|BQ468807 HM02G11r HM Hordeum vulgare subsp. vulgare cDNA clone HM02G11
5-PRIME, mRNA sequence
Length = 558
Score = 106 bits (227), Expect(2) = 2e-025
Identities = 43/57 (75%), Positives = 47/57 (82%)
Frame = +2 / +1
Query: 407 DKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLVVE 577
D+YDYA+VVVGE PYAET GDNLNLTIP PGP V + VC KCVVVL+SGR LVVE
Sbjct: 124 DEYDYAVVVVGEQPYAETAGDNLNLTIPGPGPEVIRKVCELVKCVVVLVSGRPLVVE 294
Score = 29.3 bits (58), Expect(2) = 2e-025
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +2 / +1
Query: 335 GIEATVDPSTQVVYSESPDSGVLADKYD 418
G+++ VD T + YSE PD G ++ D
Sbjct: 43 GLKSAVDKKTVIDYSEHPDKGNVSKSED 126
>gb|BG366042.2|BG366042 HVSMEi0004P02f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0004P02f, mRNA sequence
Length = 914
Score = 114 bits (245), Expect = 6e-025
Identities = 49/77 (63%), Positives = 62/77 (80%)
Frame = +2 / +1
Query: 2 PMSRIDDAVYRILRVKFTMGLFENPYPDSSLAGELGKQEHRELAREAVRKSLVLLKNGKS 181
PMSRI+DAVYRIL+VKFTMGLFE+PY D SL GELGKQEHR+LAREA ++ + + + K+
Sbjct: 583 PMSRINDAVYRILKVKFTMGLFESPYADPSLIGELGKQEHRDLAREAGKEVIGVAEKRKN 762
Query: 182 SYAPLLPLPKKAGKILV 232
++ PKKAGKIL+
Sbjct: 763 CLPLIVGFPKKAGKILL 813
Score = 99 bits (213), Expect = 7e-021
Identities = 49/76 (64%), Positives = 52/76 (68%)
Frame = -3 / -1
Query: 228 RILPAFFGRGSNGA*EDLPFFRRTRDFLTASRASSRCSCFPSSPARLESG*GFSNRPMVN 49
RILPAFFG+ + + F L ASRA SRCSCFPSSP RL S *G SNRPMVN
Sbjct: 809 RILPAFFGKPTMSGRQFFLFSATPMTSLPASRARSRCSCFPSSPMRLGSA*GLSNRPMVN 630
Query: 48 LTRRIL*TASSILLMG 1
T RIL*TAS ILLMG
Sbjct: 629 FTLRIL*TASLILLMG 582
>gb|CA026644.1|CA026644 HZ56I06r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ56I06
5-PRIME, mRNA sequence
Length = 324
Score = 108 bits (232), Expect = 4e-023
Identities = 45/72 (62%), Positives = 54/72 (75%)
Frame = +2 / +3
Query: 140 AVRKSLVLLKNGKSSYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAG 319
A + ++L S+ APLLPLPKKA KILVAGSHA++LG QCGGWTI WQG +G TT G
Sbjct: 108 ARKSLVLLKNGKTSTDAPLLPLPKKAPKILVAGSHADNLGYQCGGWTIEWQGDTGRTTVG 287
Query: 320 TTILSGIEATVD 355
TTIL ++A VD
Sbjct: 288 TTILEAVKAAVD 323
Score = 65.3 bits (137), Expect(2) = 3e-024
Identities = 29/56 (51%), Positives = 32/56 (57%)
Frame = -3 / -3
Query: 354 STVASIPERIXXXXXXXXXXPCHVIVHPPHWLPRSLAWLPATRILPAFFGRGSNGA 187
ST A R+ PCH +V PPHW PR AWLPATRIL AF G G +GA
Sbjct: 322 STAAFTASRMVVPTVVRPVSPCHSMVQPPHW*PRLSAWLPATRILGAFLGSGRSGA 155
Score = 64.4 bits (135), Expect(2) = 3e-024
Identities = 30/49 (61%), Positives = 31/49 (63%)
Frame = -3 / -3
Query: 198 SNGA*EDLPFFRRTRDFLTASRASSRCSCFPSSPARLESG*GFSNRPMV 52
S + E LPFF T DFL AS A S CSCFPS A S *GFS RPMV
Sbjct: 163 SGASVEVLPFFSSTSDFLAASLAKSLCSCFPSCSAMAGSA*GFSKRPMV 17
>gb|CA028903.1|CA028903 HZ63J10r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ63J10
5-PRIME, mRNA sequence
Length = 361
Score = 88.1 bits (187), Expect(2) = 4e-024
Identities = 36/55 (65%), Positives = 41/55 (74%)
Frame = +2 / +2
Query: 413 YDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXLVVE 577
+ YAIV VGE PY ET GDNLNLTIP PG S Q+VCG +C VLISGR +VV+
Sbjct: 101 FSYAIVAVGEHPYTETKGDNLNLTIPEPGLSTVQAVCGGVRCATVLISGRPVVVQ 265
Score = 43.0 bits (88), Expect(2) = 4e-024
Identities = 16/26 (61%), Positives = 22/26 (84%)
Frame = +2 / +2
Query: 317 GTTILSGIEATVDPSTQVVYSESPDS 394
GTTIL ++A VDPST VV++E+PD+
Sbjct: 2 GTTILEAVKAAVDPSTVVVFAENPDA 79
>gb|AV833400.1|AV833400 AV833400 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags8b19, mRNA sequence
Length = 540
Score = 87.2 bits (185), Expect(3) = 8e-024
Identities = 35/63 (55%), Positives = 46/63 (73%)
Frame = +2 / +1
Query: 389 DSGVLADKYDYAIVVVGEPPYAETFGDNLNLTIPAPGPSVXQSVCGXAKCVVVLISGRXL 568
DS + + + +AIVVVGE PYAET GDN +LTI PGP ++VC KCVVV++SGR +
Sbjct: 133 DSFMKKNDFSFAIVVVGETPYAETVGDNTDLTILDPGPDTIRTVCSAVKCVVVIVSGRPV 312
Query: 569 VVE 577
V+E
Sbjct: 313 VIE 321
Score = 34.3 bits (69), Expect(3) = 8e-024
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = +2 / +3
Query: 293 GSSGNTTAGTTILSGIEATVDPSTQVVYSESP 388
GS TTIL I++TV +T VVYSE P
Sbjct: 33 GSGXILQLXTTILXAIKSTVGDATPVVYSEXP 128
Score = 26.6 bits (52), Expect(3) = 8e-024
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +2 / +2
Query: 266 CGGWTITWQG 295
CGGW+I W G
Sbjct: 2 CGGWSIQWXG 31
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.316 0.137 0.438
Matrix: BLOSUM62
Number of Hits to DB: 251,954,212
Number of Sequences: 312970
Number of extensions: 4051804
Number of successful extensions: 91141
Number of sequences better than 1.0e-020: 67
length of database: 58,378,179
effective HSP length: 46
effective length of database: 43,981,559
effective search space used: 6421307614
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.6 bits)