TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2751069.2.1
(940 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU996988.1|BU996988 HI06I11r HI Hordeum vulgare subsp. v... 208 1e-098
gb|BE455905.3|BE455905 HVSMEg0017F08f Hordeum vulgare pre-a... 157 9e-083
gb|BQ469857.1|BQ469857 HX01B05T HX Hordeum vulgare subsp. v... 233 2e-066
gb|BF258030.2|BF258030 HVSMEf0014I19f Hordeum vulgare seedl... 243 8e-064
gb|CA032516.1|CA032516 HX13F16r HX Hordeum vulgare subsp. v... 198 4e-050
gb|BF624205.2|BF624205 HVSMEa0012L04f Hordeum vulgare seedl... 112 3e-049
gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA li... 126 2e-028
gb|BE231148.2|BE231148 HVSMEg0017A24f Hordeum vulgare pre-a... 122 4e-027
>gb|BU996988.1|BU996988 HI06I11r HI Hordeum vulgare subsp. vulgare cDNA clone HI06I11
5-PRIME, mRNA sequence
Length = 656
Score = 208 bits (448), Expect(3) = 1e-098
Identities = 88/111 (79%), Positives = 98/111 (88%)
Frame = -2 / +1
Query: 570 VVACNSTKKIRNTSNAGITFGAPGGIICAKASMLLQSVPPAVLVRFLREHRSEWADYNID 391
V+ ++KKIR+ + A F APGG+ICAKASMLLQSVPPAVLVRFLREHRSEWADYN D
Sbjct: 136 VIIACNSKKIRSNNTAPNAFIAPGGVICAKASMLLQSVPPAVLVRFLREHRSEWADYNFD 315
Query: 390 AYLASSLKTSACSLPGLRPMRFSGGQMIMPLAHTVENEEILEVVRLEGQPL 238
AY AS+LK+S+CSLPGLRPMRFSG Q+IMPLAHTVENEEILEVVRLEGQ L
Sbjct: 316 AYSASALKSSSCSLPGLRPMRFSGSQIIMPLAHTVENEEILEVVRLEGQAL 468
Score = 147 bits (315), Expect(3) = 1e-079
Identities = 65/101 (64%), Positives = 73/101 (72%)
Frame = +2 / -1
Query: 245 CPXXXXXXXXXXXXXV*ASGMIICPPENLMGRNPGSEHALVFNDEAKYASIL*SAHSDLC 424
CP V*ASGM+I PENL+GRNPGSEH L F+ EA+YAS L*SAHSD C
Sbjct: 461 CPSRRTTSRISSFSTV*ASGMMIWLPENLIGRNPGSEHELDFSAEAEYASKL*SAHSDRC 282
Query: 425 SLRNRTSTAGGTLCSNMDAFAQIMPPGAPNVIPALLVFLIF 547
SLRNRTSTAGGTLCSN+DA A I PPGA + A+L+ LIF
Sbjct: 281 SLRNRTSTAGGTLCSNIDALAHITPPGAIKALGAVLLLLIF 159
Score = 134 bits (287), Expect(3) = 3e-049
Identities = 56/93 (60%), Positives = 66/93 (70%)
Frame = -3 / +2
Query: 548 RKLGILAMLGLHLEPQEALFVRRHPCYCRASHRQYWYDF*GSIDLNGLITILMRIWLHH* 369
R+LG + +L + L E L+V +H CYCR H+QYW+DF*G+I LNGLI LM I L *
Sbjct: 158 RRLGAITLLPMLL*LLEVLYVLKHQCYCRVFHQQYWFDF*GNIGLNGLIITLMHIRLQR* 337
Query: 368 RPVRAHFLGCDP*DFLGGR*SCHLLTQWRTRRF 270
V AHFLGC *DFLG R SCHLLT+WR RRF
Sbjct: 338 NQVHAHFLGCAL*DFLGARSSCHLLTRWRMRRF 436
Score = 131 bits (280), Expect(3) = 3e-064
Identities = 61/104 (58%), Positives = 69/104 (66%)
Frame = +1 / -3
Query: 238 KRLSFKADNFKNLLVLHCVSKWHDHLPPRKSHGSQPRK*ARTGLQ**SQIRINIVISPFR 417
+RL FK +NF NLL+LH VSKWHD L PRKSH +QPRK*A T Q *S+I I ++ISPFR
Sbjct: 468 ERLPFKTNNF*NLLILHRVSKWHDDLAPRKSHRAQPRK*A*T*FQR*SRICIKVIISPFR 289
Query: 418 SMLPQKSYQYCRWDALQ*HGCLRTNNASWGSKCNPSIASIPNFL 549
M P KS QYC W+ LQ*H C T N S K S PN L
Sbjct: 288 PMFP*KSNQYCWWNTLQ*H*CFSTYNTSRSYKSIGSSVIAPNLL 157
Score = 126 bits (270), Expect(3) = 1e-098
Identities = 53/58 (91%), Positives = 54/58 (93%)
Frame = -2 / +1
Query: 231 DEALLSRDIHLLQLCTGIDEKSVGSSFQLVFAPIDEHFPDDAPLISSGFRVIPLDMKT 58
DE LLSRDIHLLQ CTGIDEKS+GS FQLVFAPIDE FPDDAPLISSGFRVIPLDMKT
Sbjct: 469 DEGLLSRDIHLLQFCTGIDEKSMGSCFQLVFAPIDELFPDDAPLISSGFRVIPLDMKT 642
Score = 125 bits (268), Expect(2) = 2e-042
Identities = 58/104 (55%), Positives = 72/104 (69%)
Frame = -1 / +3
Query: 550 QEN*EY*QCWDYIWSPRRHYLCEGIHVTAERPTGSTGTISEGA*I*MG*LQY*CVFGFII 371
+E+*E * C +S R Y+C I+VTAE T STG+IS+G +*MG*L *C+FGF
Sbjct: 156 KED*EQ*HCSQCFYSSWRCYMC*SINVTAECSTSSTGSISKGTSV*MG*L*L*CIFGFSA 335
Query: 370 EDQCVLTSWVATHEIFWGADDHATCSHSGERGDS*SCPP*RTTS 239
E + +LTSWVA +EIFW D HATCSH GE GDS SC *R ++
Sbjct: 336 EIKFMLTSWVAPYEIFWEPDHHATCSHGGE*GDSRSCSS*RASA 467
Score = 119 bits (255), Expect = 2e-026
Identities = 52/86 (60%), Positives = 59/86 (68%)
Frame = -2 / +1
Query: 699 ALGRQPAVLRTFSQRLSRGFNDAISGFNDDGWSVMGGDGIEDVVVACNSTKKIRNTSNAG 520
ALGRQPAVLRTFSQRLSRGFNDAISGFNDDGWSVM GDGIEDV++ACNS K N +
Sbjct: 10 ALGRQPAVLRTFSQRLSRGFNDAISGFNDDGWSVMAGDGIEDVIIACNSKKIRSNNTAPN 189
Query: 519 ITFGAPGGIICAKASMLLQSVPPAVL 442
G I + +L P ++
Sbjct: 190 AFIAPGGVICAKASMLLQSVPPAVLV 267
Score = 107 bits (228), Expect(3) = 1e-079
Identities = 43/59 (72%), Positives = 53/59 (89%)
Frame = +2 / -1
Query: 56 TVFISSGMTRKPEEINGASSGKCSSIGANTSWKEEPTDFSSIPVQSWRRWMSLERRASS 232
+VF+SSG+TRKPE+INGASSGK SSIGA TSWK++P DFSSIPVQ+ RW+SL++R SS
Sbjct: 644 SVFMSSGITRKPEDINGASSGKSSSIGAKTSWKQDPIDFSSIPVQN*SRWISLDKRPSS 468
Score = 94.5 bits (200), Expect(3) = 1e-037
Identities = 50/82 (60%), Positives = 53/82 (64%)
Frame = +3 / -2
Query: 243 VVLQGGQLQESPRSPLCEQVA*SSAPQKISWVATQEVSTHWSSMMKPNTHQYCNQPIQIY 422
+ LQ QL ESP SP CEQVA* S QKIS ATQEVS + S +KPN HQ NQPIQ
Sbjct: 463 LALQDEQLLESPHSPPCEQVA**SGSQKIS*GATQEVSMNLISALKPNMHQSYNQPIQTD 284
Query: 423 APSEIVPVLPVGRSAVTWMPSH 488
P EI PVL V SAVT M H
Sbjct: 283 VPLEIEPVLLVEHSAVTLML*H 218
Score = 85.4 bits (180), Expect(3) = 3e-064
Identities = 35/58 (60%), Positives = 44/58 (75%)
Frame = +1 / -3
Query: 58 CFHIKWYDTKARGNQWSIIRKMLINWCKHKLEGGTHRFLIYSSAKLEKVDVP*KKGFI 231
CFH++WY+T+A WSIIR+ LINW K KLE G H FL+YSSAKL++VD+ * K I
Sbjct: 642 CFHVQWYNTEA*RY*WSIIREKLINWGKDKLEAGPH*FLVYSSAKLKQVDIS**KTLI 469
Score = 82.2 bits (173), Expect(3) = 1e-079
Identities = 37/57 (64%), Positives = 41/57 (71%)
Frame = +2 / -1
Query: 530 LVFLIFLVELQAXXXXXXXXXXITDQPSSLKPLMASLNPLLSL*LKVRRTAGCLPKA 700
++ L+ ELQA ITDQPSSLKPL+ASLNPLLSL*L VRRTAGCLP A
Sbjct: 179 VLLLLIFFELQAIITSSMPSPAITDQPSSLKPLIASLNPLLSL*LNVRRTAGCLPSA 9
Score = 73.9 bits (155), Expect(3) = 3e-049
Identities = 31/52 (59%), Positives = 37/52 (71%)
Frame = -1 / +3
Query: 214 KGHPPSPALHWNR*EICGFLLPACVCTN**AFSG*CSIDFLWLSCHTT*YEN 59
K +PP+ LHWNR EI G LLP C+C N**AF *CSI+ L C+TT +EN
Sbjct: 486 KRYPPASVLHWNRREINGVLLPTCLCPN**AFP**CSINIFRLPCYTTGHEN 641
Score = 68.4 bits (143), Expect(3) = 3e-064
Identities = 32/48 (66%), Positives = 35/48 (72%)
Frame = +1 / -3
Query: 553 *VASNNNVFNAISPHYRPTIIIETTNGIIKPPA*SLTKGP*NCRLPSQ 696
*VAS+N++FNAIS HYRPTIIIE T IIK LTK P N RLP Q
Sbjct: 156 *VASDNHIFNAISGHYRPTIIIEATYSIIKSSTQPLTKCPQNSRLPPQ 13
Score = 67.5 bits (141), Expect(2) = 2e-042
Identities = 34/59 (57%), Positives = 39/59 (66%)
Frame = -3 / +2
Query: 230 MKPFFQGTSTFSSFALE*MRNLWVPPSSLCLHQLMSIFRMMLH*FPLAFVSYHLI*KQL 54
M+ F+Q ST SFALE* RN W P S+L L QLMS MMLH*+ A V YH *KQ+
Sbjct: 470 MRVFYQEISTCFSFALE*TRNQWGPASNLSLPQLMSFSLMMLH*YLQASVLYHWT*KQM 646
Score = 65.7 bits (137), Expect(3) = 1e-037
Identities = 32/58 (55%), Positives = 34/58 (58%)
Frame = +3 / -2
Query: 57 LFSYQVV*HESQRKSMEHHPENAHQLVQTQAGRRNPQISHLFQCKAGEGGCPLKEGLH 230
LFS VV*H S + MEHH AHQL Q Q G R P IS LFQCK GG L + H
Sbjct: 643 LFSCPVV*HGSLKILMEHHQGKAHQLGQRQVGSRTPLISRLFQCKTEAGGYLLIKDPH 470
Score = 65.2 bits (136), Expect(3) = 1e-098
Identities = 28/50 (56%), Positives = 36/50 (72%)
Frame = -1 / +3
Query: 694 GKATCSSTDL*SKTKQGV**CH*WFQ**WLVCNGGRWH*RRCCCLQLNQE 545
G+A C S D+*S+ + +**C+ W Q**WLVCNG RWH*R LQL ++
Sbjct: 15 GEAACCSADI*SEAE*RI**CYKWLQ**WLVCNGRRWH*RCDYRLQLKED 164
Score = 34.5 bits (69), Expect(3) = 1e-037
Identities = 14/20 (70%), Positives = 15/20 (75%)
Frame = +3 / -2
Query: 546 SWLSCKQQQRLQCHLPPLQT 605
S LSCK+ LQCHL PLQT
Sbjct: 163 SSLSCKR*SHLQCHLRPLQT 104
Score = 25.8 bits (50), Expect(3) = 3e-049
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = -3 / +2
Query: 596 WGEMALKTLLLLATQPR 546
W EMALK L LATQ R
Sbjct: 113 WPEMALKM*LSLATQRR 163
>gb|BE455905.3|BE455905 HVSMEg0017F08f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0017F08f, mRNA sequence
Length = 806
Score = 157 bits (338), Expect(3) = 9e-083
Identities = 64/88 (72%), Positives = 75/88 (85%)
Frame = -2 / +1
Query: 813 EVLRPLYESSRVVAQKMTTVALRHLRQIAQETSGEVVYALGRQPAVLRTFSQRLSRGFND 634
EVLRPLYES +++AQKMT ALRH+RQIA E+SGE+ Y GRQPAVLRTFSQRLSRGFND
Sbjct: 1 EVLRPLYESPKILAQKMTIAALRHIRQIAHESSGEMPYGGGRQPAVLRTFSQRLSRGFND 180
Query: 633 AISGFNDDGWSVMGGDGIEDVVVACNST 550
A++GF DDGWS+M DG EDV +A NS+
Sbjct: 181 AVNGFLDDGWSLMSSDGAEDVTIAINSS 264
Score = 96.8 bits (205), Expect(3) = 9e-083
Identities = 39/73 (53%), Positives = 52/73 (71%)
Frame = -2 / +2
Query: 306 MPLAHTVENEEILEVVRLEGQPLTHDEALLSRDIHLLQLCTGIDEKSVGSSFQLVFAPID 127
+PLAHT+E+EE+ E +RL G L HDE+ LSRD++LLQ+C G+ E + + QLVFAPID
Sbjct: 518 LPLAHTLEHEEVFEGIRLXGHSLWHDESCLSRDMYLLQVCNGVHENAACACAQLVFAPID 697
Query: 126 EHFPDDAPLISSG 88
E DD L+ G
Sbjct: 698 EIVADDCSLLPXG 736
Score = 92.3 bits (195), Expect(3) = 9e-083
Identities = 38/61 (62%), Positives = 47/61 (77%)
Frame = -2 / +1
Query: 501 GGIICAKASMLLQSVPPAVLVRFLREHRSEWADYNIDAYLASSLKTSACSLPGLRPMRFS 322
GG++CAKA MLLQ+VPPA+LVRFLREHRSEWAD +DAY ++L+ S + GLR F
Sbjct: 322 GGVLCAKAFMLLQNVPPALLVRFLREHRSEWADPCVDAYXRAALRASPYAXXGLRASWFM 501
Query: 321 G 319
G
Sbjct: 502 G 504
Score = 76.7 bits (161), Expect(3) = 2e-035
Identities = 45/90 (50%), Positives = 50/90 (55%)
Frame = +2 / -1
Query: 542 IFLVELQAXXXXXXXXXXITDQPSSLKPLMASLNPLLSL*LKVRRTAGCLPKAYTTSPLV 721
+F EL A I+DQPSS PL ASL PLL+L*L V +TAG P SPL
Sbjct: 272 LFGDELIAIVTSSAPSLLISDQPSSRNPLTASLKPLLNL*LNVLKTAGWRPPP*GISPLD 93
Query: 722 S*AICLRWRSATVVIF*ATTLEDSYSGRST 811
S AICL R A +VIF A DS SG T
Sbjct: 92 SCAICLM*RRAAIVIFCARIFGDS*SGLRT 3
Score = 72.5 bits (152), Expect(3) = 5e-025
Identities = 39/85 (45%), Positives = 48/85 (56%)
Frame = +1 / -3
Query: 553 *VASNNNVFNAISPHYRPTIIIETTNGIIKPPA*SLTKGP*NCRLPSQGIHYFSTCFLSN 732
*V SN N+ + I+ H P II ++ N II+ A SLTK *NC L I +FST F+ N
Sbjct: 261 *VDSNCNILSTITAHQ*PAIIQKSINSIIETSAQSLTKCS*NCWLAPPSIGHFST*FMCN 82
Query: 733 LSKVAQCHSSHFLSNYSRRLI*WSK 807
L V Q + H L RLI WSK
Sbjct: 81 LPNVTQSSNCHLLRKNLWRLIKWSK 7
Score = 63.4 bits (132), Expect(3) = 2e-035
Identities = 31/61 (50%), Positives = 33/61 (54%)
Frame = +2 / -1
Query: 320 PENLMGRNPGSEHALVFNDEAKYASIL*SAHSDLCSLRNRTSTAGGTLCSNMDAFAQIMP 499
P N + R P L AS SAHS+ CSLRNRTS AGGT CSNM A A P
Sbjct: 503 PINQLARRPXXA*GLARKAARX*ASTQGSAHSERCSLRNRTSRAGGTFCSNMKALAHKTP 324
Query: 500 P 502
P
Sbjct: 323 P 321
Score = 47.8 bits (98), Expect(3) = 2e-035
Identities = 26/65 (40%), Positives = 33/65 (50%)
Frame = +2 / -3
Query: 110 SSGKCSSIGANTSWKEEPTDFSSIPVQSWRRWMSLERRASS*VRGCPXXXXXXXXXXXXX 289
SS SSIGA TS + FS P+ + RR++SL+R+ SS R CP
Sbjct: 714 SSATISSIGAKTSCAQAQAAFSWTPLHTCRRYISLDRQLSSCQRLCPSSLIPSKTSSCSK 535
Query: 290 V*ASG 304
V ASG
Sbjct: 534 VCASG 520
Score = 40.0 bits (81), Expect(3) = 5e-025
Identities = 24/48 (50%), Positives = 28/48 (58%)
Frame = +1 / -3
Query: 358 RTGLQ**SQIRINIVISPFRSMLPQKSYQYCRWDALQ*HGCLRTNNAS 501
RT Q + I IN ISPFR+MLPQK Y R LQ H L T +A+
Sbjct: 465 RTSSQSSAXISINTGISPFRAMLPQKPY**SRRYILQQHESLGT*DAT 322
Score = 39.6 bits (80), Expect(3) = 5e-025
Identities = 24/79 (30%), Positives = 40/79 (50%)
Frame = +1 / -2
Query: 76 YDTKARGNQWSIIRKMLINWCKHKLEGGTHRFLIYSSAKLEKVDVP*KKGFIMSKRLSFK 255
+ K G Q +II IN K KL T L+ + A L+K+ + * FIM + +S +
Sbjct: 748 FTRKPXG*Q*AIISNDFINRRKDKLCTSTSSILMDTVAYLQKIHIS**TTFIMPETVSXE 569
Query: 256 ADNFKNLLVLHCVSKWHDH 312
++ K+ +L + KW +
Sbjct: 568 SNTLKDFFML*GMCKWQGY 512
>gb|BQ469857.1|BQ469857 HX01B05T HX Hordeum vulgare subsp. vulgare cDNA clone HX01B05
5-PRIME, mRNA sequence
Length = 658
Score = 233 bits (503), Expect(2) = 2e-066
Identities = 90/122 (73%), Positives = 107/122 (87%)
Frame = -2 / +3
Query: 915 AEMLPSGYLVRPCEGXGSIVHIVDHLDLEAWSVPEVLRPLYESSRVVAQKMTTVALRHLR 736
AE+LPSGYL+RPCEG GS++HIVDH+DL+AWSVPEVLRPLYES ++VAQKMT ALRH+R
Sbjct: 180 AEVLPSGYLIRPCEGGGSMIHIVDHVDLDAWSVPEVLRPLYESPKIVAQKMTIAALRHIR 359
Query: 735 QIAQETSGEVVYALGRQPAVLRTFSQRLSRGFNDAISGFNDDGWSVMGGDGIEDVVVACN 556
QIA E+SGE+ Y GRQPAVLRTFSQRLSRGFNDA+SGF DDGWS++ DG ED+ + N
Sbjct: 360 QIAHESSGEIPYGAGRQPAVLRTFSQRLSRGFNDAVSGFPDDGWSLLTSDGAEDITITVN 539
Query: 555 ST 550
S+
Sbjct: 540 SS 545
Score = 126 bits (269), Expect(2) = 2e-032
Identities = 66/105 (62%), Positives = 71/105 (67%)
Frame = +2 / -1
Query: 602 DQPSSLKPLMASLNPLLSL*LKVRRTAGCLPKAYTTSPLVS*AICLRWRSATVVIF*ATT 781
DQPSS PL ASL PLLSL LKV RTAG P SPL S AICL RSA +VIF AT
Sbjct: 493 DQPSSGNPLTASLKPLLSLWLKVLRTAGWRPAP*GISPLDSCAICLMCRSAAIVIFCATI 314
Query: 782 LEDSYSGRSTSGTLHASRSRWSTICTIEPXPSHGRTKYPLGSISA 916
DS SG TSGTL AS+S ST+ +EP PSHGR + PLGS SA
Sbjct: 313 FGDS*SGLRTSGTLQASKST*STMWIMEPPPSHGRIR*PLGSTSA 179
Score = 40.0 bits (81), Expect(2) = 2e-066
Identities = 15/18 (83%), Positives = 17/18 (94%)
Frame = -2 / +3
Query: 501 GGIICAKASMLLQSVPPA 448
GGI+CAKASML+Q VPPA
Sbjct: 603 GGILCAKASMLVQDVPPA 656
Score = 33.6 bits (67), Expect(2) = 2e-032
Identities = 12/18 (66%), Positives = 15/18 (83%)
Frame = +2 / -1
Query: 449 AGGTLCSNMDAFAQIMPP 502
AGGT C+++DAFA MPP
Sbjct: 655 AGGTSCTSIDAFAHKMPP 602
>gb|BF258030.2|BF258030 HVSMEf0014I19f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0014I19f, mRNA sequence
Length = 563
Score = 243 bits (526), Expect = 8e-064
Identities = 99/145 (68%), Positives = 121/145 (83%)
Frame = -2 / +3
Query: 492 ICAKASMLLQSVPPAVLVRFLREHRSEWADYNIDAYLASSLKTSACSLPGLRPMRFSGGQ 313
+CAKASMLLQ+VPPA+LVRFLREHRSEWAD +DAY A++L+ S ++PGLR F G Q
Sbjct: 3 LCAKASMLLQNVPPALLVRFLREHRSEWADPGVDAYSAAALRASPYAVPGLRASGFMGSQ 182
Query: 312 MIMPLAHTVENEEILEVVRLEGQPLTHDEALLSRDIHLLQLCTGIDEKSVGSSFQLVFAP 133
+I+PLAHT+E+EE LEV+RLEG L HDE +LSRD++LLQLC+G+DE + G+ QLVFAP
Sbjct: 183 VILPLAHTLEHEEFLEVIRLEGHSLCHDEVVLSRDMYLLQLCSGVDENAAGACAQLVFAP 362
Query: 132 IDEHFPDDAPLISSGFRVIPLDMKT 58
IDE F DDAPL+ SGFRVIPLD KT
Sbjct: 363 IDESFADDAPLLPSGFRVIPLDAKT 437
Score = 139 bits (299), Expect = 2e-032
Identities = 71/144 (49%), Positives = 86/144 (59%)
Frame = +2 / -2
Query: 56 TVFISSGMTRKPEEINGASSGKCSSIGANTSWKEEPTDFSSIPVQSWRRWMSLERRASS* 235
+V SSG+TRKPE +GASS SSIGA TS + P FSS P+ + RR++SL+R SS*
Sbjct: 439 SVLASSGITRKPEGSSGASSANDSSIGAKTSCAQAPAAFSSTPLHNCRRYISLDRTTSS* 260
Query: 236 VRGCPXXXXXXXXXXXXXV*ASGMIICPPENLMGRNPGSEHALVFNDEAKYASIL*SAHS 415
R CP V ASG I P N + R PG+ L A+ AS SAHS
Sbjct: 259 QRLCPSSLITSKNSSCSKVCASGRIT*LPINPLARRPGTA*GLARKAAAE*ASTPGSAHS 80
Query: 416 DLCSLRNRTSTAGGTLCSNMDAFA 487
+ CSLRNRTS AGGT CSN++A A
Sbjct: 79 ERCSLRNRTSRAGGTFCSNIEALA 8
Score = 112 bits (239), Expect = 3e-024
Identities = 63/141 (44%), Positives = 80/141 (56%)
Frame = +1 / -1
Query: 67 IKWYDTKARGNQWSIIRKMLINWCKHKLEGGTHRFLIYSSAKLEKVDVP*KKGFIMSKRL 246
IKWY T+ARG QWSII K IN K KL T LI ++A L+K+ + * FIM++ +
Sbjct: 428 IKWYHTEARG*QWSIISKRFINRRKDKLCTSTSSILIDTAA*LQKIHIS**NNFIMTETV 249
Query: 247 SFKADNFKNLLVLHCVSKWHDHLPPRKSHGSQPRK*ARTGLQ**SQIRINIVISPFRSML 426
S +++N + L +L + KW D+L K S R RT Q I IN ISPFR+ML
Sbjct: 248 SLESNNLQELFML*GMCKWQDNLTAHKPTRS*TRNCIRTSSQSSGGISINTRISPFRAML 69
Query: 427 PQKSYQYCRWDALQ*HGCLRT 489
PQK Y R LQ H L T
Sbjct: 68 PQKPY**SRRYILQQH*SLGT 6
>gb|CA032516.1|CA032516 HX13F16r HX Hordeum vulgare subsp. vulgare cDNA clone HX13F16
5-PRIME, mRNA sequence
Length = 639
Score = 198 bits (427), Expect = 4e-050
Identities = 78/98 (79%), Positives = 90/98 (91%)
Frame = -2 / +2
Query: 915 AEMLPSGYLVRPCEGXGSIVHIVDHLDLEAWSVPEVLRPLYESSRVVAQKMTTVALRHLR 736
AE+LPSGYL+RPCEG GS++HIVDH+DL+AWSVPEVLRPLYES ++VAQKMT ALRH+R
Sbjct: 344 AEVLPSGYLIRPCEGGGSMIHIVDHVDLDAWSVPEVLRPLYESPKIVAQKMTIAALRHIR 523
Query: 735 QIAQETSGEVVYALGRQPAVLRTFSQRLSRGFNDAISG 622
QIA E+SGE+ Y GRQPAVLRTFSQRLSRGFNDA+SG
Sbjct: 524 QIAHESSGEIPYGAGRQPAVLRTFSQRLSRGFNDAVSG 637
Score = 116 bits (247), Expect = 2e-025
Identities = 61/98 (62%), Positives = 66/98 (67%)
Frame = +2 / -1
Query: 623 PLMASLNPLLSL*LKVRRTAGCLPKAYTTSPLVS*AICLRWRSATVVIF*ATTLEDSYSG 802
PL ASL PLLSL LKV RTAG P SPL S AICL RSA +VIF AT DS SG
Sbjct: 636 PLTASLKPLLSLWLKVLRTAGWRPAP*GISPLDSCAICLMCRSAAIVIFCATIFGDS*SG 457
Query: 803 RSTSGTLHASRSRWSTICTIEPXPSHGRTKYPLGSISA 916
TSGTL AS+S ST+ +EP PSHGR + PLGS SA
Sbjct: 456 LRTSGTLQASKST*STMWIMEPPPSHGRIR*PLGSTSA 343
>gb|BF624205.2|BF624205 HVSMEa0012L04f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0012L04f, mRNA sequence
Length = 486
Score = 112 bits (239), Expect(2) = 3e-049
Identities = 48/85 (56%), Positives = 63/85 (74%)
Frame = -2 / +2
Query: 501 GGIICAKASMLLQSVPPAVLVRFLREHRSEWADYNIDAYLASSLKTSACSLPGLRPMRFS 322
GG++CAKASMLLQ+VPPA+LVRFLREHRSEWAD +DAY A++L+ S ++PGLR F
Sbjct: 230 GGVLCAKASMLLQNVPPALLVRFLREHRSEWADPGVDAYSAAALRASPYAVPGLRASGFM 409
Query: 321 GGQMIMPLAHTVENEEILEVVRLEG 247
G Q+I+ L E + ++ LEG
Sbjct: 410 GSQVILALHIP*SIESL*XLLDLEG 484
Score = 103 bits (219), Expect(2) = 3e-049
Identities = 41/57 (71%), Positives = 48/57 (84%)
Frame = -2 / +2
Query: 720 TSGEVVYALGRQPAVLRTFSQRLSRGFNDAISGFNDDGWSVMGGDGIEDVVVACNST 550
+SGE+ Y GRQPAVLRTFSQRLSRGFNDA++GF DDGWS+M DG EDV +A NS+
Sbjct: 2 SSGEMPYGGGRQPAVLRTFSQRLSRGFNDAVNGFLDDGWSLMSSDGAEDVTIAINSS 172
Score = 68.9 bits (144), Expect(2) = 3e-021
Identities = 32/61 (52%), Positives = 38/61 (62%)
Frame = +2 / -1
Query: 320 PENLMGRNPGSEHALVFNDEAKYASIL*SAHSDLCSLRNRTSTAGGTLCSNMDAFAQIMP 499
P N + R PG+ L A+ AS SAHS+ CSLRNRTS AGGT CSN++A A P
Sbjct: 411 PINPLARRPGTA*GLARKAAAE*ASTPGSAHSERCSLRNRTSRAGGTFCSNIEALAHKTP 232
Query: 500 P 502
P
Sbjct: 231 P 229
Score = 52.8 bits (109), Expect(2) = 3e-021
Identities = 29/59 (49%), Positives = 33/59 (55%)
Frame = +2 / -1
Query: 542 IFLVELQAXXXXXXXXXXITDQPSSLKPLMASLNPLLSL*LKVRRTAGCLPKAYTTSPL 718
+F EL A I+DQPSS PL ASL PLL+L*L V +TAG P SPL
Sbjct: 180 LFGDELIAIVTSSAPSLLISDQPSSRNPLTASLKPLLNL*LNVLKTAGWRPPP*GISPL 4
>gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38k12 5', mRNA sequence
Length = 633
Score = 126 bits (270), Expect = 2e-028
Identities = 53/58 (91%), Positives = 54/58 (93%)
Frame = -2 / +1
Query: 231 DEALLSRDIHLLQLCTGIDEKSVGSSFQLVFAPIDEHFPDDAPLISSGFRVIPLDMKT 58
DE LLSRDIHLLQ CTGIDEKS+GS FQLVFAPIDE FPDDAPLISSGFRVIPLDMKT
Sbjct: 43 DEGLLSRDIHLLQFCTGIDEKSMGSCFQLVFAPIDELFPDDAPLISSGFRVIPLDMKT 216
Score = 107 bits (228), Expect = 1e-022
Identities = 43/59 (72%), Positives = 53/59 (89%)
Frame = +2 / -2
Query: 56 TVFISSGMTRKPEEINGASSGKCSSIGANTSWKEEPTDFSSIPVQSWRRWMSLERRASS 232
+VF+SSG+TRKPE+INGASSGK SSIGA TSWK++P DFSSIPVQ+ RW+SL++R SS
Sbjct: 218 SVFMSSGITRKPEDINGASSGKSSSIGAKTSWKQDPIDFSSIPVQN*SRWISLDKRPSS 42
>gb|BE231148.2|BE231148 HVSMEg0017A24f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0017A24f, mRNA sequence
Length = 905
Score = 122 bits (260), Expect = 4e-027
Identities = 49/70 (70%), Positives = 58/70 (82%)
Frame = -2 / +3
Query: 267 EVVRLEGQPLTHDEALLSRDIHLLQLCTGIDEKSVGSSFQLVFAPIDEHFPDDAPLISSG 88
EV+RLEG L HDE +LSRD++LLQLC+G+DE + G+ QLVFAPIDE F DDAPL+ SG
Sbjct: 3 EVIRLEGHSLCHDEVVLSRDMYLLQLCSGVDENAAGACAQLVFAPIDESFADDAPLLPSG 182
Query: 87 FRVIPLDMKT 58
FRVIPLD KT
Sbjct: 183 FRVIPLDAKT 212
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 489,773,633
Number of Sequences: 312970
Number of extensions: 8223048
Number of successful extensions: 48215
Number of sequences better than 1.0e-020: 10
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 10946972140
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)