TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750663.2.1
(1282 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ764079.1|BQ764079 EBro03_SQ006_P05_R root, 3 week, wat... 244 e-114
gb|BM375019.2|BM375019 EBem06_SQ002_B16_R embryo, 21 DPA, n... 346 1e-094
gb|BQ763699.1|BQ763699 EBro03_SQ005_N01 _R root, 3 week, wa... 244 2e-085
gb|BG415911.2|BG415911 HVSMEk0009A02f Hordeum vulgare testa... 161 8e-039
gb|AV917400.1|AV917400 AV917400 K. Sato unpublished cDNA li... 80 1e-027
gb|AV922578.1|AV922578 AV922578 K. Sato unpublished cDNA li... 80 4e-022
>gb|BQ764079.1|BQ764079 EBro03_SQ006_P05_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ006_P05 5', mRNA sequence
Length = 717
Score = 244 bits (528), Expect(3) = e-114
Identities = 105/111 (94%), Positives = 109/111 (98%)
Frame = -1 / +2
Query: 1282 IVQSTVMPCALVEKAIFGLLRICQRLLPYKENLADELLRSLQLVLKLDARVADAYCENIT 1103
IVQSTVMPCALVEKAIFGLLRICQRLLPYKENLAD+LLRSLQLVLKLDARVADAYCENIT
Sbjct: 8 IVQSTVMPCALVEKAIFGLLRICQRLLPYKENLADDLLRSLQLVLKLDARVADAYCENIT 187
Query: 1102 QEVARLVKANAAHIKSQMGWRTVILLLSITARHPDASEVGFEAIVFIMTEG 950
QEVARLVKANA HIKSQMGWRTV+LLLSITARHPDAS VGFEAI+FIM+EG
Sbjct: 188 QEVARLVKANAGHIKSQMGWRTVVLLLSITARHPDASGVGFEAIMFIMSEG 340
Score = 235 bits (508), Expect(3) = 2e-098
Identities = 100/110 (90%), Positives = 103/110 (93%)
Frame = +3 / -1
Query: 951 PSVMMNTIASKPTSEASGWRAVMESSNITVLQPICDFICAAFALTSRATSCVMFSQYASA 1130
PS M+N IASKPT EASGWRAVMESSN TVLQPICDF+C AFALTSRATSCVMFSQYASA
Sbjct: 339 PSDMINMIASKPTPEASGWRAVMESSNTTVLQPICDFMCPAFALTSRATSCVMFSQYASA 160
Query: 1131 TRASSLRTNCNDLSNSSARFSLYGSNLWHILRSPKIAFSTRAHGITVD*T 1280
TRASSLRTNCNDLS SSARFSLYGSNLWHI RSPKIAFSTRAHG+TVD*T
Sbjct: 159 TRASSLRTNCNDLSKSSARFSLYGSNLWHIRRSPKIAFSTRAHGMTVD*T 10
Score = 182 bits (393), Expect(3) = 6e-072
Identities = 73/108 (67%), Positives = 87/108 (80%)
Frame = +2 / -3
Query: 959 HDEHDSLKTDFGSIWMACCNGEQQYYCSPAHL*LYMCRICFDEPCNLLCDVLTIRICYPC 1138
HD+HDSLK SIW+A CNGEQQ+Y SPAHL* Y+ RICFD+PCNLL +VLT+ ICY C
Sbjct: 331 HDKHDSLKAYS*SIWVASCNGEQQHYSSPAHL*FYVSRICFDKPCNLLRNVLTVCICYTC 152
Query: 1139 VELEN*LQRPQ*FISKILLVW*QPLAYPQESKNSFLHESTRHNCGLNY 1282
++LEN*LQ PQ +SK+L VW PLAYPQ+SKNSFLH+ H+ GLNY
Sbjct: 151 IQLEN*LQ*PQQVVSKVLFVWK*PLAYPQKSKNSFLHKGAWHDRGLNY 8
Score = 181 bits (389), Expect(3) = 4e-062
Identities = 74/110 (67%), Positives = 88/110 (80%)
Frame = -3 / +1
Query: 1280 SSVHSYAVCSRGESYFWTPEDMPEVATIQGESC**ITEVVAVSSQARRTGSRCVL*EHHT 1101
SSVH +A+ GESYFWT DMPEV +IQ E C* + EV+A+SSQA T SRC+L*EH+
Sbjct: 10 SSVHGHAMRPCGESYFWTSADMPEVTSIQREPC*RLAEVIAISSQAGCTCSRCIL*EHYA 189
Query: 1100 GGCTARQSKCGTYKVTDGLENSNIAALHYSTPSRCFRSRF*GYRVHHDRG 951
GCTA QSKCGT+K+TDGLEN ++AALHYS+P RCFRSR *GY V+H RG
Sbjct: 190 RGCTACQSKCGTHKITDGLENCSVAALHYSSPPRCFRSRL*GYHVYHVRG 339
Score = 153 bits (329), Expect(3) = 1e-049
Identities = 71/110 (64%), Positives = 78/110 (70%)
Frame = -2 / +3
Query: 1281 *FSPQLCRVLSWRKLFLDS*GYARGCYHTRRILLMNY*GRCS*FSSSTHG*QMRIVRTSH 1102
*FSP+ C WRKLFLD GYARG +HT+R LL *G C+*FSS H *QM VRT
Sbjct: 9 *FSPRSCHAPLWRKLFLDFCGYARGYFHTKRTLLTTC*GHCN*FSSWMHV*QMHTVRTLR 188
Query: 1101 RRLHGSSKQMRHI*SHRWAGEQ*YCCSPLQHAIQMLPKSVLRLSCSS*QR 952
+RLHG SKQMR *+HRWAGE * CCSPLQ A QML + LRLSC S QR
Sbjct: 189 KRLHGLSKQMRDT*NHRWAGEL*CCCSPLQLATQMLQE*ALRLSCLSCQR 338
Score = 147 bits (316), Expect(3) = 2e-045
Identities = 76/117 (64%), Positives = 84/117 (71%)
Frame = +1 / -2
Query: 931 SLVKGEPPLS**TR*PQNRLRKHLDGVL*WRAAILLFSSPSVTLYVPHLL*RAVQPPV*C 1110
+L+ PL+**T *PQ+ L KHL G L*WRAA L FSSPSV L VPHLL*+AVQP *C
Sbjct: 359 NLILRGDPLT**T**PQSLLLKHLGGEL*WRAATLQFSSPSVILCVPHLL*QAVQPLA*C 180
Query: 1111 SHNTHLLPVRRA*ELTATTSVIHQQDSPCMVATSGISSGVQK*LSPREHTA*LWTEL 1281
SH+ HLL V A*EL A TS QQ S CM TSGIS+ VQK*LSP+ A* WTEL
Sbjct: 179 SHSMHLLHVHPA*ELIAMTSASRQQGSLCMEVTSGISAEVQK*LSPQGRMA*PWTEL 9
Score = 112 bits (240), Expect(3) = e-114
Identities = 47/72 (65%), Positives = 53/72 (73%)
Frame = -1 / +2
Query: 784 EEGEKGPEAIREMWXXXXXXXXXXXLDQREEVRNHALASLQRCLTSTGELCLQSATWSHA 605
EE ++G EAIREMW LDQREEVRNHAL SLQRCLT+T +CLQ TWSHA
Sbjct: 500 EEADRGMEAIREMWLKLLQALKKLSLDQREEVRNHALVSLQRCLTATEGICLQPTTWSHA 679
Query: 604 FDLVIFSLLDDL 569
FD +IF+LLDDL
Sbjct: 680 FDHIIFALLDDL 715
Score = 95.0 bits (201), Expect(3) = e-114
Identities = 38/58 (65%), Positives = 46/58 (79%)
Frame = -1 / +2
Query: 970 VFIMTEGAHLSLANYGFCIDAARQFAESRVGLADRSVRALDLMSDSVRNLALWSQEIK 797
+ + HLS +NY FCI+A+RQFAESRVGL DRS+RALDLM+DSV NLA WSQ+ K
Sbjct: 317 IMFIMSEGHLSKSNYAFCIEASRQFAESRVGLTDRSIRALDLMADSVTNLARWSQDTK 490
Score = 83.5 bits (176), Expect(3) = 2e-098
Identities = 39/72 (54%), Positives = 49/72 (68%)
Frame = +2 / -3
Query: 569 QIIEQGEYDEVKGMGPRRGLEAEFSRGC*ASLERSQRMVSHLLPLIQAQFLQCLQQPEPH 748
Q+++Q EYD +K + P GLE C*ASLE +Q +VSHLL L+QAQF Q LQQ EPH
Sbjct: 715 QVVQQREYDVIKSV*PCGGLETYPFGRC*ASLE*NQCVVSHLLSLVQAQFFQRLQQFEPH 536
Query: 749 LPYRLRPFLAFF 784
LP P ++FF
Sbjct: 535 LPDCFHPSISFF 500
Score = 80.8 bits (170), Expect(3) = 2e-098
Identities = 37/56 (66%), Positives = 44/56 (78%)
Frame = +3 / -1
Query: 780 SSNVVAFISWDHKARFLTESDIKSRARTDLSAKPTLDSANCRAASMQKP*FASER* 947
+S+ +F+S DH+AR +TES IKSRAR DLS +PTLDSANC ASMQK *F ER*
Sbjct: 507 ASSPGSFVSCDHRARLVTESAIKSRARMDLSVRPTLDSANCLDASMQKA*FDFER* 340
Score = 65.7 bits (137), Expect(3) = 6e-072
Identities = 27/42 (64%), Positives = 28/42 (66%)
Frame = +3 / -1
Query: 567 SKSSSKENMTRSKAWDHVADWRQSSPVDVRHRWREASAWFRT 692
SKSSS NM SKA DHV WR V VRHRW E +AWFRT
Sbjct: 717 SKSSSNANMM*SKACDHVVGWRHIPSVAVRHRWSETNAWFRT 592
Score = 62.5 bits (130), Expect(3) = 6e-072
Identities = 24/45 (53%), Positives = 32/45 (71%)
Frame = +2 / -3
Query: 797 FYLLGPQGKVSDGVGHQVQGTDRPIS*TNSRLGKLPCRIDAEAIV 931
F +L P GK+SD +GHQ+QG D P+ *TN LGKL R +AE ++
Sbjct: 490 FCILRPPGKISDRIGHQIQGADGPVC*TNPGLGKLSRRFNAEGVI 356
Score = 56.5 bits (117), Expect(3) = 4e-062
Identities = 26/50 (52%), Positives = 28/50 (56%)
Frame = -2 / +3
Query: 717 N*AWIRGRRCETMRWLLSSDA*HPRENSASSPRRGPMPLTSSYSPCSMIC 568
N*AW R RRCET W SSDA* + SSP G L +SYS C C
Sbjct: 567 N*AWTRERRCETTHWFHSSDA*QRPKGYVSSPPHGHTLLITSYSRCWTTC 716
Score = 47.3 bits (97), Expect(3) = 1e-049
Identities = 24/64 (37%), Positives = 30/64 (46%)
Frame = -3 / +1
Query: 770 RAGGDKGDVAQXXXXXXXXXXXXXXXXAKPCAGFSPAMPNIHGRTLPPVRDVVPCL*PRH 591
R G ++GDVAQ AKP GF+PAMPN R + P +V * H
Sbjct: 514 RDGSNQGDVAQTAASAEKTELGPERGGAKPRIGFTPAMPNSDRRDMSPAHHMVTRF*SHH 693
Query: 590 ILLA 579
I +A
Sbjct: 694 IRVA 705
Score = 41.8 bits (85), Expect(3) = 2e-045
Identities = 20/47 (42%), Positives = 29/47 (61%)
Frame = +1 / -2
Query: 811 TTRQGF*RSRTSSPGHGQTYQLNQL*TRQTAVPHRCRSHSSLVKGEP 951
TT Q * +R S+PG G T L+Q TRQT +CR + +++G+P
Sbjct: 476 TTGQD**PNRPSNPGRGWTCLLDQPWTRQTVSTLQCRRRNLILRGDP 336
Score = 40.5 bits (82), Expect(3) = 4e-062
Identities = 23/51 (45%), Positives = 25/51 (49%)
Frame = -3 / +1
Query: 950 GSPFTSELWLLHRCGTAVCRV*SWFS**VCPCPGLDVRLRQKPCLVVPRDK 798
GSP +L LLH VCRV W + V P PG D R C VV R K
Sbjct: 337 GSPLKIKLRLLH*SVETVCRVQGWSNRQVHPRPGFDGRFGH*SCPVVARYK 489
Score = 35.0 bits (70), Expect(3) = 1e-049
Identities = 20/37 (54%), Positives = 20/37 (54%)
Frame = -2 / +3
Query: 921 SASMRHGSLPSLELV*LIGLSVPWT*CPTPSETLPCG 811
SA R SLPS LV* G S PW * P S LP G
Sbjct: 366 SALKRRDSLPSPGLV*QTGPSAPWI*WPIRSLILPGG 476
Score = 32.2 bits (64), Expect(3) = 2e-045
Identities = 19/41 (46%), Positives = 22/41 (53%)
Frame = +1 / -2
Query: 568 ANHRARRI*RGQRHGTTSRTGGRVLPWMLGIAGEKPAHGFA 690
A+ A RI* Q+ T G L +LGIAG KP GFA
Sbjct: 716 ASRPATRI*CDQKRVTMWWAGDISLRSLLGIAGVKPMRGFA 594
>gb|BM375019.2|BM375019 EBem06_SQ002_B16_R embryo, 21 DPA, no treatment, cv Optic, EBem06
Hordeum vulgare subsp. vulgare cDNA clone
EBem06_SQ002_B16 5', mRNA sequence
Length = 551
Score = 346 bits (750), Expect = 1e-094
Identities = 141/178 (79%), Positives = 159/178 (89%)
Frame = -1 / +2
Query: 688 RNHALASLQRCLTSTGELCLQSATWSHAFDLVIFSLLDDLLEISQNHSQKDYRNMEGSLV 509
RNHAL SLQRCLT+T +CLQ TWSHAFD +IF+LLDDLLEI+QNHSQKD+RNMEGSLV
Sbjct: 2 RNHALVSLQRCLTATEGICLQPTTWSHAFDHIIFALLDDLLEIAQNHSQKDHRNMEGSLV 181
Query: 508 LAMKLVAKVYLQLLPDLFGLSSFCKLWLGVLSRMEKYVKVKVRGKRSDRLQEVVPDLLKN 329
LA+KLVAK YLQLLPDLFGLSSFCKLWLGVLSRMEK +K KVRGKRSD+LQE++PDLL+N
Sbjct: 182 LAVKLVAKAYLQLLPDLFGLSSFCKLWLGVLSRMEKCIKTKVRGKRSDKLQELIPDLLRN 361
Query: 328 ILLVMKSKGILAKRSTIGGDSLWELTWLHAHNISASLLPDVFPSQEYEQQSSTGSPRG 155
IL+ MK +GILAKRSTIGGDSLWELTWLH +NIS L +VFP+QEYE S+ GSPRG
Sbjct: 362 ILVAMKGRGILAKRSTIGGDSLWELTWLHVNNISTGLQSEVFPTQEYEPPSNAGSPRG 535
Score = 197 bits (424), Expect = 1e-049
Identities = 98/178 (55%), Positives = 120/178 (67%)
Frame = +2 / -2
Query: 155 PSWATGXXXXXXXXXWKNIRQQTSRDVVRVKPRQLPQAVAPDRAPLRKDALALHHEQNVL 334
PS ATG +++R Q RDVV V+P QLPQAVAP RAPL +D ALH +Q+V
Sbjct: 535 PSRATGIARRLILLGREDLRLQARRDVVHVEPGQLPQAVAPYRAPLGEDTPALHRDQDVS 356
Query: 335 EQVRDHLLQPVAPLASDLDLDVLLHPAQDPQPQLAETA*AEKVRQELEVYLRNQLHGEHK 514
+VRD LL+ VA LA+DL LD LLHPA+DPQPQLAE A E+V +L+V L Q HGEH+
Sbjct: 355 *KVRDQLLKLVAALATDLGLDALLHPAEDPQPQLAEAAQPEEVW*QLKVGLCYQFHGEHE 176
Query: 515 *PFHVSVVLLGVVLADLQQIIEQGEYDEVKGMGPRRGLEAEFSRGC*ASLERSQRMVS 688
FHV VV L VVL D QQ+++Q EYD +K + P GLE C*ASLE +Q +VS
Sbjct: 175 GSFHVPVVFL*VVLGDFQQVVQQREYDVIKSV*PCGGLETYPFGRC*ASLE*NQCVVS 2
Score = 160 bits (344), Expect(2) = 9e-059
Identities = 70/97 (72%), Positives = 72/97 (74%)
Frame = +3 / -3
Query: 399 YFSIRLRTPSHSLQKLLRPKRSGKSWRYTFATSFMASTSDPSMFL*SFWEWFWLISSKSS 578
+FSI LRTPSHSLQKLL PKRSG S R FATSF AST DPSMFL SF EWFW ISSKSS
Sbjct: 291 HFSILLRTPSHSLQKLLSPKRSGNS*R*AFATSFTASTRDPSMFLWSFCEWFWAISSKSS 112
Query: 579 SKENMTRSKAWDHVADWRQSSPVDVRHRWREASAWFR 689
S NM SKA DHV WR V VRHRW E +AWFR
Sbjct: 111 SNANMM*SKACDHVVGWRHIPSVAVRHRWSETNAWFR 1
Score = 139 bits (298), Expect(2) = 4e-050
Identities = 65/116 (56%), Positives = 73/116 (62%)
Frame = -2 / +3
Query: 687 ETMRWLLSSDA*HPRENSASSPRRGPMPLTSSYSPCSMICWRSARTTPRRTTETWKGHLC 508
ET W SSDA* + SSP G L +SYS C CW+S +TT R+TT TWK C
Sbjct: 3 ETTHWFHSSDA*QRPKGYVSSPPHGHTLLITSYSRCWTTCWKSPKTTHRKTTGTWKDPSC 182
Query: 507 SP*SWLRRYTSSSCRTFSA*AVSASCGWGS*AGWRSTSRSRSEASGATGCRRWSRT 340
SP*+W +R T S +T S *A SASCGWGS AGWRS SR RS AS AT R SRT
Sbjct: 183 SP*NW*QRPTFSCYQTSSG*AASASCGWGSSAGWRSASRPRSVASAATSFRS*SRT 350
Score = 115 bits (245), Expect(2) = 7e-031
Identities = 50/100 (50%), Positives = 64/100 (64%)
Frame = -3 / +1
Query: 686 KPCAGFSPAMPNIHGRTLPPVRDVVPCL*PRHILLAR*FAGDQPEPLPEGLQKHGRVTCA 507
KP GF+PAMPN R + P +V * HI +A AG++P+PL E Q+HGR+ A
Sbjct: 4 KPRIGFTPAMPNSDRRDMSPAHHMVTRF*SHHIRVAGRLAGNRPKPLTERPQEHGRIPRA 183
Query: 506 RHEAGCEGIPPALAGPFRPKQFLQAVAGGPEPDGEVRQGQ 387
R E G +G+P A+ P R +Q LQAVAGGP+ DGEV Q Q
Sbjct: 184 RRETGSKGLPSAVTRPLRAEQLLQAVAGGPQQDGEVHQDQ 303
Score = 87.7 bits (185), Expect(2) = 9e-059
Identities = 39/54 (72%), Positives = 42/54 (77%)
Frame = +3 / -3
Query: 186 SYSWLGKTSGSRLAEMLCA*SHVSSHRLSPPIVLLFARMPLLFITSRMFLSRSG 347
SYSW+GKTS R EML S VSSHRLSPPIVLL AR+PL FI +RMFL RSG
Sbjct: 504 SYSWVGKTSDCRPVEMLFTWSQVSSHRLSPPIVLLLARIPLPFIATRMFLRRSG 343
Score = 79.9 bits (168), Expect(2) = 4e-050
Identities = 36/58 (62%), Positives = 40/58 (68%)
Frame = -3 / +1
Query: 329 HSARDEEQGHPCEEEHDRGRQPVGADVASRAQHLG*SAA*CFSKPGVRATEQHR*PKR 156
H RDE QG+P +EEHDRGRQPVGAD+A R QHL A PGV A EQ R*P+R
Sbjct: 361 HPGRDEGQGYPRQEEHDRGRQPVGADLAPREQHLDGPAIGGLPDPGV*AAEQCR*PER 534
Score = 42.3 bits (86), Expect(2) = 7e-031
Identities = 21/37 (56%), Positives = 23/37 (62%)
Frame = -2 / +3
Query: 264 CGS*RGFTRTTSRLVCCLMFFQARSTSNRAAPVAQEG 154
CGS* G T TTSR C + RS S RA PVA+EG
Sbjct: 426 CGS*PGST*TTSRRACNRRSSRPRSMSRRAMPVAREG 536
>gb|BQ763699.1|BQ763699 EBro03_SQ005_N01 _R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ005_N01 5', mRNA sequence
Length = 546
Score = 244 bits (528), Expect(2) = 2e-085
Identities = 105/111 (94%), Positives = 109/111 (98%)
Frame = -1 / +1
Query: 1282 IVQSTVMPCALVEKAIFGLLRICQRLLPYKENLADELLRSLQLVLKLDARVADAYCENIT 1103
IVQSTVMPCALVEKAIFGLLRICQRLLPYKENLAD+LLRSLQLVLKLDARVADAYCENIT
Sbjct: 25 IVQSTVMPCALVEKAIFGLLRICQRLLPYKENLADDLLRSLQLVLKLDARVADAYCENIT 204
Query: 1102 QEVARLVKANAAHIKSQMGWRTVILLLSITARHPDASEVGFEAIVFIMTEG 950
QEVARLVKANA HIKSQMGWRTV+LLLSITARHPDAS VGFEAI+FIM+EG
Sbjct: 205 QEVARLVKANAGHIKSQMGWRTVVLLLSITARHPDASGVGFEAIMFIMSEG 357
Score = 235 bits (508), Expect(2) = 2e-078
Identities = 100/110 (90%), Positives = 103/110 (93%)
Frame = +3 / -2
Query: 951 PSVMMNTIASKPTSEASGWRAVMESSNITVLQPICDFICAAFALTSRATSCVMFSQYASA 1130
PS M+N IASKPT EASGWRAVMESSN TVLQPICDF+C AFALTSRATSCVMFSQYASA
Sbjct: 356 PSDMINMIASKPTPEASGWRAVMESSNTTVLQPICDFMCPAFALTSRATSCVMFSQYASA 177
Query: 1131 TRASSLRTNCNDLSNSSARFSLYGSNLWHILRSPKIAFSTRAHGITVD*T 1280
TRASSLRTNCNDLS SSARFSLYGSNLWHI RSPKIAFSTRAHG+TVD*T
Sbjct: 176 TRASSLRTNCNDLSKSSARFSLYGSNLWHIRRSPKIAFSTRAHGMTVD*T 27
Score = 182 bits (393), Expect(2) = 6e-058
Identities = 73/108 (67%), Positives = 87/108 (80%)
Frame = +2 / -1
Query: 959 HDEHDSLKTDFGSIWMACCNGEQQYYCSPAHL*LYMCRICFDEPCNLLCDVLTIRICYPC 1138
HD+HDSLK SIW+A CNGEQQ+Y SPAHL* Y+ RICFD+PCNLL +VLT+ ICY C
Sbjct: 348 HDKHDSLKAYS*SIWVASCNGEQQHYSSPAHL*FYVSRICFDKPCNLLRNVLTVCICYTC 169
Query: 1139 VELEN*LQRPQ*FISKILLVW*QPLAYPQESKNSFLHESTRHNCGLNY 1282
++LEN*LQ PQ +SK+L VW PLAYPQ+SKNSFLH+ H+ GLNY
Sbjct: 168 IQLEN*LQ*PQQVVSKVLFVWK*PLAYPQKSKNSFLHKGAWHDRGLNY 25
Score = 181 bits (389), Expect(2) = 1e-049
Identities = 74/110 (67%), Positives = 88/110 (80%)
Frame = -3 / +3
Query: 1280 SSVHSYAVCSRGESYFWTPEDMPEVATIQGESC**ITEVVAVSSQARRTGSRCVL*EHHT 1101
SSVH +A+ GESYFWT DMPEV +IQ E C* + EV+A+SSQA T SRC+L*EH+
Sbjct: 27 SSVHGHAMRPCGESYFWTSADMPEVTSIQREPC*RLAEVIAISSQAGCTCSRCIL*EHYA 206
Query: 1100 GGCTARQSKCGTYKVTDGLENSNIAALHYSTPSRCFRSRF*GYRVHHDRG 951
GCTA QSKCGT+K+TDGLEN ++AALHYS+P RCFRSR *GY V+H RG
Sbjct: 207 RGCTACQSKCGTHKITDGLENCSVAALHYSSPPRCFRSRL*GYHVYHVRG 356
Score = 153 bits (329), Expect(2) = 6e-039
Identities = 71/110 (64%), Positives = 78/110 (70%)
Frame = -2 / +2
Query: 1281 *FSPQLCRVLSWRKLFLDS*GYARGCYHTRRILLMNY*GRCS*FSSSTHG*QMRIVRTSH 1102
*FSP+ C WRKLFLD GYARG +HT+R LL *G C+*FSS H *QM VRT
Sbjct: 26 *FSPRSCHAPLWRKLFLDFCGYARGYFHTKRTLLTTC*GHCN*FSSWMHV*QMHTVRTLR 205
Query: 1101 RRLHGSSKQMRHI*SHRWAGEQ*YCCSPLQHAIQMLPKSVLRLSCSS*QR 952
+RLHG SKQMR *+HRWAGE * CCSPLQ A QML + LRLSC S QR
Sbjct: 206 KRLHGLSKQMRDT*NHRWAGEL*CCCSPLQLATQMLQE*ALRLSCLSCQR 355
Score = 147 bits (316), Expect(2) = 4e-040
Identities = 76/117 (64%), Positives = 84/117 (71%)
Frame = +1 / -3
Query: 931 SLVKGEPPLS**TR*PQNRLRKHLDGVL*WRAAILLFSSPSVTLYVPHLL*RAVQPPV*C 1110
+L+ PL+**T *PQ+ L KHL G L*WRAA L FSSPSV L VPHLL*+AVQP *C
Sbjct: 376 NLILRGDPLT**T**PQSLLLKHLGGEL*WRAATLQFSSPSVILCVPHLL*QAVQPLA*C 197
Query: 1111 SHNTHLLPVRRA*ELTATTSVIHQQDSPCMVATSGISSGVQK*LSPREHTA*LWTEL 1281
SH+ HLL V A*EL A TS QQ S CM TSGIS+ VQK*LSP+ A* WTEL
Sbjct: 196 SHSMHLLHVHPA*ELIAMTSASRQQGSLCMEVTSGISAEVQK*LSPQGRMA*PWTEL 26
Score = 92.7 bits (196), Expect(2) = 2e-085
Identities = 37/58 (63%), Positives = 46/58 (79%)
Frame = -1 / +1
Query: 970 VFIMTEGAHLSLANYGFCIDAARQFAESRVGLADRSVRALDLMSDSVRNLALWSQEIK 797
+ + HLS +NY FCI+A+RQFAESRVGL DRS+RAL+LM+DSV NLA WSQ+ K
Sbjct: 334 IMFIMSEGHLSKSNYAFCIEASRQFAESRVGLTDRSIRALNLMADSVTNLARWSQDTK 507
Score = 78.0 bits (164), Expect(2) = 2e-078
Identities = 36/56 (64%), Positives = 43/56 (76%)
Frame = +3 / -2
Query: 780 SSNVVAFISWDHKARFLTESDIKSRARTDLSAKPTLDSANCRAASMQKP*FASER* 947
+S+ +F+S DH+AR +TES IK RAR DLS +PTLDSANC ASMQK *F ER*
Sbjct: 524 ASSPGSFVSCDHRARLVTESAIKFRARMDLSVRPTLDSANCLDASMQKA*FDFER* 357
Score = 62.5 bits (130), Expect(2) = 6e-058
Identities = 24/45 (53%), Positives = 32/45 (71%)
Frame = +2 / -1
Query: 797 FYLLGPQGKVSDGVGHQVQGTDRPIS*TNSRLGKLPCRIDAEAIV 931
F +L P GK+SD +GHQ+QG D P+ *TN LGKL R +AE ++
Sbjct: 507 FCILRPPGKISDRIGHQIQGADGPVC*TNPGLGKLSRRFNAEGVI 373
Score = 38.2 bits (77), Expect(2) = 4e-040
Identities = 19/47 (40%), Positives = 28/47 (59%)
Frame = +1 / -3
Query: 811 TTRQGF*RSRTSSPGHGQTYQLNQL*TRQTAVPHRCRSHSSLVKGEP 951
TT Q * +R S+ G G T L+Q TRQT +CR + +++G+P
Sbjct: 493 TTGQD**PNRPSNSGRGWTCLLDQPWTRQTVSTLQCRRRNLILRGDP 353
Score = 36.8 bits (74), Expect(2) = 1e-049
Identities = 22/51 (43%), Positives = 24/51 (47%)
Frame = -3 / +3
Query: 950 GSPFTSELWLLHRCGTAVCRV*SWFS**VCPCPGLDVRLRQKPCLVVPRDK 798
GSP +L LLH VCRV W + V P P D R C VV R K
Sbjct: 354 GSPLKIKLRLLH*SVETVCRVQGWSNRQVHPRPEFDGRFGH*SCPVVARYK 506
Score = 28.1 bits (55), Expect(2) = 6e-039
Identities = 19/37 (51%), Positives = 19/37 (51%)
Frame = -2 / +2
Query: 921 SASMRHGSLPSLELV*LIGLSVPWT*CPTPSETLPCG 811
SA R SLPS LV* G S P * P S LP G
Sbjct: 383 SALKRRDSLPSPGLV*QTGPSAP*I*WPIRSLILPGG 493
>gb|BG415911.2|BG415911 HVSMEk0009A02f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0009A02f, mRNA sequence
Length = 985
Score = 161 bits (346), Expect = 8e-039
Identities = 66/138 (47%), Positives = 97/138 (70%)
Frame = -1 / +3
Query: 553 NHSQKDYRNMEGSLVLAMKLVAKVYLQLLPDLFGLSSFCKLWLGVLSRMEKYVKVKVRGK 374
++S KD+ NM+ SL+ A+KL+ KV+LQ L DL SSF KLWL VL +EK++KVK+RG+
Sbjct: 3 SYSPKDFGNMDVSLLHAVKLLCKVFLQSLKDLSAQSSFSKLWLEVLDMIEKFMKVKLRGR 182
Query: 373 RSDRLQEVVPDLLKNILLVMKSKGILAKRSTIGGDSLWELTWLHAHNISASLLPDVFPSQ 194
R+++L E +P+LLKNILLVMK+ G+L+K S ++LWE TWL + I+ S+ P+VFP
Sbjct: 183 RTEKLHEAIPELLKNILLVMKANGVLSKTSASEENTLWETTWLQVNKIAPSMQPEVFPDN 362
Query: 193 EYEQQSSTGSPRGPSPVE 140
E + + + SP +
Sbjct: 363 ESDSAAKDEQSKSDSPAQ 416
>gb|AV917400.1|AV917400 AV917400 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags18m03 5', mRNA sequence
Length = 656
Score = 80.3 bits (169), Expect(3) = 1e-027
Identities = 32/63 (50%), Positives = 44/63 (69%)
Frame = -1 / +1
Query: 379 GKRSDRLQEVVPDLLKNILLVMKSKGILAKRSTIGGDSLWELTWLHAHNISASLLPDVFP 200
G R++RLQE +LLKN+LLVMK+ G+L++ S G SLWE TWLH + ++ SL VFP
Sbjct: 355 GSRTERLQETATELLKNMLLVMKASGVLSRTSAGGESSLWEATWLHVNKMAPSLQSVVFP 534
Query: 199 SQE 191
+
Sbjct: 535 DYD 543
Score = 45.1 bits (92), Expect(3) = 1e-027
Identities = 23/55 (41%), Positives = 26/55 (47%)
Frame = -2 / +2
Query: 588 SPCSMICWRSARTTPRRTTETWKGHLCSP*SWLRRYTSSSCRTFSA*AVSASCGW 424
S CS C R R + + +ETWK C *S Y S F AVS SCGW
Sbjct: 143 SSCSTNC*RLRRISRQSISETWKLPSCML*SCYPNYPCSHSTIFRRTAVSVSCGW 307
Score = 36.3 bits (73), Expect(3) = 1e-027
Identities = 14/22 (63%), Positives = 16/22 (72%)
Frame = -1 / +1
Query: 706 DQREEVRNHALASLQRCLTSTG 641
DQREEVR+HAL L RC+ G
Sbjct: 31 DQREEVRDHALVLLHRCIVVDG 96
>gb|AV922578.1|AV922578 AV922578 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags18m03 3', mRNA sequence
Length = 713
Score = 80.3 bits (169), Expect(2) = 4e-022
Identities = 32/63 (50%), Positives = 44/63 (69%)
Frame = -1 / -2
Query: 379 GKRSDRLQEVVPDLLKNILLVMKSKGILAKRSTIGGDSLWELTWLHAHNISASLLPDVFP 200
G R++RLQE +LLKN+LLVMK+ G+L++ S G SLWE TWLH + ++ SL VFP
Sbjct: 481 GSRTERLQETATELLKNMLLVMKASGVLSRTSAGGESSLWEATWLHVNKMAPSLQSVVFP 302
Query: 199 SQE 191
+
Sbjct: 301 DYD 293
Score = 45.1 bits (92), Expect(2) = 4e-022
Identities = 23/55 (41%), Positives = 26/55 (47%)
Frame = -2 / -3
Query: 588 SPCSMICWRSARTTPRRTTETWKGHLCSP*SWLRRYTSSSCRTFSA*AVSASCGW 424
S CS C R R + + +ETWK C *S Y S F AVS SCGW
Sbjct: 693 SSCSTNC*RLRRISRQSISETWKLPSCML*SCYPNYPCSHSTIFRRTAVSVSCGW 529
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 654,113,493
Number of Sequences: 312970
Number of extensions: 11647137
Number of successful extensions: 91233
Number of sequences better than 1.0e-020: 6
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 15587956837
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)