TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750560.2.1
(660 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CB883217.1|CB883217 HQ01I24w HQ Hordeum vulgare subsp. v... 144 3e-034
gb|BE214385.1|BE214385 HV_CEb0003E19f Hordeum vulgare seedl... 142 2e-033
gb|BG345137.1|BG345137 HVSMEg0018N15f Hordeum vulgare pre-a... 138 2e-032
gb|BQ470466.1|BQ470466 HX03B03r HX Hordeum vulgare subsp. v... 76 4e-027
gb|CA024140.1|CA024140 HZ48H14r HZ Hordeum vulgare subsp. v... 72 1e-025
gb|AV933277.1|AV933277 AV933277 K. Sato unpublished cDNA li... 65 1e-021
>gb|CB883217.1|CB883217 HQ01I24w HQ Hordeum vulgare subsp. vulgare cDNA clone HQ01I24
3-PRIME, mRNA sequence
Length = 476
Score = 144 bits (310), Expect = 3e-034
Identities = 54/78 (69%), Positives = 68/78 (87%)
Frame = +3 / +3
Query: 273 FRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPPKILCKVVN 452
+ ELWHACAGPLVTVPR G+LV+YFPQGH+EQ+EAS +Q + L+DLPPK+LC+V+N
Sbjct: 243 YDELWHACAGPLVTVPRVGDLVFYFPQGHIEQVEASMNQVAGNQMRLYDLPPKLLCRVIN 422
Query: 453 VELRAETDSDEVYAQIML 506
VEL+AE D+DEVYAQ+ML
Sbjct: 423 VELKAEADTDEVYAQVML 476
>gb|BE214385.1|BE214385 HV_CEb0003E19f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0003E19f, mRNA sequence
Length = 720
Score = 142 bits (305), Expect = 2e-033
Identities = 53/86 (61%), Positives = 70/86 (81%)
Frame = +3 / +2
Query: 264 DALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPPKILCK 443
D L+ ELWHACAGPLVT PR G+LV+YFPQGH+EQ+EAS +Q + L+DLPPK+LC+
Sbjct: 122 DPLYDELWHACAGPLVTAPRVGDLVFYFPQGHIEQVEASMNQVAGNQMRLYDLPPKLLCR 301
Query: 444 VVNVELRAETDSDEVYAQIMLQPEAD 521
V+N+EL+AE D D+VYAQ++L E +
Sbjct: 302 VINIELKAEEDIDKVYAQVILMLELE 379
>gb|BG345137.1|BG345137 HVSMEg0018N15f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0018N15f, mRNA sequence
Length = 831
Score = 138 bits (297), Expect = 2e-032
Identities = 52/86 (60%), Positives = 69/86 (80%)
Frame = +3 / +1
Query: 264 DALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPPKILCK 443
D L+ ELWHACA PLVT PR G+LV+YFPQGH+EQ+EAS +Q + L+DLPPK+LC+
Sbjct: 208 DPLYDELWHACAVPLVTAPRVGDLVFYFPQGHIEQVEASMNQVAGNQMRLYDLPPKLLCR 387
Query: 444 VVNVELRAETDSDEVYAQIMLQPEAD 521
V+N+EL+AE D D+VYAQ++L E +
Sbjct: 388 VINIELKAEADIDKVYAQVILMLELE 465
>gb|BQ470466.1|BQ470466 HX03B03r HX Hordeum vulgare subsp. vulgare cDNA clone HX03B03
5-PRIME, mRNA sequence
Length = 632
Score = 76.2 bits (160), Expect(2) = 4e-027
Identities = 28/47 (59%), Positives = 38/47 (80%)
Frame = +3 / +1
Query: 384 DQQLDQHLPLFDLPPKILCKVVNVELRAETDSDEVYAQIMLQPEADQ 524
+Q + L+DLPPK+LC+V+NVEL+AE D+DEVYAQ+ML PE +Q
Sbjct: 16 NQVAGNQMRLYDLPPKLLCRVINVELKAEADTDEVYAQVMLMPEPEQ 156
Score = 64.8 bits (135), Expect(2) = 4e-027
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +3 / +1
Query: 576 VYSFCKTLTASDTSTHGGFSVLRRHAEE 659
V SFCKTLTASDTSTHGGFSVLRRHA+E
Sbjct: 217 VRSFCKTLTASDTSTHGGFSVLRRHADE 300
>gb|CA024140.1|CA024140 HZ48H14r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ48H14
5-PRIME, mRNA sequence
Length = 402
Score = 71.6 bits (150), Expect(2) = 1e-025
Identities = 28/50 (56%), Positives = 38/50 (76%)
Frame = +3 / +3
Query: 375 ASTDQQLDQHLPLFDLPPKILCKVVNVELRAETDSDEVYAQIMLQPEADQ 524
A +Q + L+DLP K+LC+V+NVEL+AE D+DEVYAQ+ML PE +Q
Sbjct: 3 ARGNQVAANQMRLYDLPSKLLCRVLNVELKAEADTDEVYAQVMLMPEPEQ 152
Score = 64.8 bits (135), Expect(2) = 1e-025
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +3 / +3
Query: 576 VYSFCKTLTASDTSTHGGFSVLRRHAEE 659
V SFCKTLTASDTSTHGGFSVLRRHA+E
Sbjct: 225 VRSFCKTLTASDTSTHGGFSVLRRHADE 308
>gb|AV933277.1|AV933277 AV933277 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal6b18 5', mRNA sequence
Length = 667
Score = 64.8 bits (135), Expect(2) = 1e-021
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +3 / +2
Query: 576 VYSFCKTLTASDTSTHGGFSVLRRHAEE 659
V SFCKTLTASDTSTHGGFSVLRRHA+E
Sbjct: 176 VRSFCKTLTASDTSTHGGFSVLRRHADE 259
Score = 57.9 bits (120), Expect(2) = 1e-021
Identities = 21/30 (70%), Positives = 27/30 (90%)
Frame = +3 / +2
Query: 435 LCKVVNVELRAETDSDEVYAQIMLQPEADQ 524
LC+V+NVEL+AE D+DEVYAQ+ML PE +Q
Sbjct: 26 LCRVINVELKAEADTDEVYAQVMLMPEPEQ 115
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 295,895,576
Number of Sequences: 312970
Number of extensions: 4050502
Number of successful extensions: 51898
Number of sequences better than 1.0e-020: 6
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7126007112
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)