TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750436.2.1
(656 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY247215.1| Hordeum vulgare subsp. vulgare syntaxin-like... 192 1e-048
gb|BG418473.2|BG418473 HVSMEk0022N01f Hordeum vulgare testa... 121 5e-027
gb|AV917182.1|AV917182 AV917182 K. Sato unpublished cDNA li... 121 5e-027
gb|BQ460377.1|BQ460377 HA09O01r HA Hordeum vulgare subsp. v... 121 5e-027
gb|AY246906.1| Hordeum vulgare subsp. vulgare syntaxin (Ror... 104 8e-027
gb|AY246907.1| Hordeum vulgare subsp. vulgare syntaxin (Ror... 104 8e-027
gb|CB880180.1|CB880180 HM05L22w HM Hordeum vulgare subsp. v... 104 8e-027
gb|CB881364.1|CB881364 HM09J09w HM Hordeum vulgare subsp. v... 103 1e-026
gb|AY247217.1| Hordeum vulgare subsp. vulgare syntaxin-like... 119 2e-026
gb|AV919917.1|AV919917 AV919917 K. Sato unpublished cDNA li... 117 5e-026
gb|CD662917.1|CD662917 UCRHV18_03ce02_b1 Drought-stressed D... 115 2e-025
>gb|AY247215.1| Hordeum vulgare subsp. vulgare syntaxin-like protein 2 gene,
partial cds
Length = 931
Score = 192 bits (414), Expect = 1e-048
Identities = 81/104 (77%), Positives = 93/104 (89%)
Frame = -1 / +1
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
IERIIS+ RGEE++ AAVAEHGKGAVLAA++EIQ R DAAREVERSLLELHQVFLDMAV+
Sbjct: 568 IERIISEGRGEEIMSAAVAEHGKGAVLAALNEIQDRHDAAREVERSLLELHQVFLDMAVV 747
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
V++QGEKLDDIE+ V NA YV GNKELGKA+++QR SRKCLC
Sbjct: 748 VQSQGEKLDDIENQVTNARDYVHSGNKELGKARQHQRGSRKCLC 879
Score = 108 bits (231), Expect(2) = 8e-028
Identities = 48/65 (73%), Positives = 51/65 (78%)
Frame = +3 / -2
Query: 462 PLRLHHDGHVEEHLVELQEAAFHLARRVTAXLDLVHRGEHGALAVLRDSGAQELLAAVVG 641
PLRLH DGHVEE LVEL+EAA HL V A LDLV RGEH ALAVL D GA +LLAA +G
Sbjct: 762 PLRLHDDGHVEEDLVELEEAALHLPGGVVAVLDLVERGEHRALAVLGDGGAHDLLAAALG 583
Query: 642 DDALD 656
DDALD
Sbjct: 582 DDALD 568
Score = 34.5 bits (69), Expect(2) = 8e-028
Identities = 14/20 (70%), Positives = 15/20 (75%)
Frame = +1 / -3
Query: 295 IGTMTSRTRSSRIMMPMQRH 354
+GTMT R R S MMPMQRH
Sbjct: 929 MGTMTMRMRRSSRMMPMQRH 870
>gb|BG418473.2|BG418473 HVSMEk0022N01f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0022N01f, mRNA sequence
Length = 821
Score = 121 bits (258), Expect = 5e-027
Identities = 51/104 (49%), Positives = 74/104 (71%)
Frame = -1 / +1
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+ +I R E++ AV + G+G VL V EIQ R DA R++ER LLEL Q+FLDMAV+
Sbjct: 238 IDDLIETGRSEQIFKDAVQQQGRGQVLDTVAEIQERHDAVRDLERKLLELQQIFLDMAVL 417
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
VE QG+ ++ IE+HV+NA++++Q G L AK+ Q++SRK +C
Sbjct: 418 VEAQGDMINHIETHVSNATNHIQQGVGALHNAKKLQKNSRKWMC 549
>gb|AV917182.1|AV917182 AV917182 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags8f06 5', mRNA sequence
Length = 555
Score = 121 bits (258), Expect = 5e-027
Identities = 51/104 (49%), Positives = 74/104 (71%)
Frame = -1 / +3
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+ +I R E++ AV + G+G VL V EIQ R DA R++ER LLEL Q+FLDMAV+
Sbjct: 192 IDDLIETGRSEQIFKDAVQQQGRGQVLDTVAEIQERHDAVRDLERKLLELQQIFLDMAVL 371
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
VE QG+ ++ IE+HV+NA++++Q G L AK+ Q++SRK +C
Sbjct: 372 VEAQGDMINHIETHVSNATNHIQQGVGALQNAKKLQKNSRKWMC 503
>gb|BQ460377.1|BQ460377 HA09O01r HA Hordeum vulgare subsp. vulgare cDNA clone HA09O01
5-PRIME, mRNA sequence
Length = 482
Score = 121 bits (258), Expect = 5e-027
Identities = 51/104 (49%), Positives = 74/104 (71%)
Frame = -1 / +3
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+ +I R E++ AV + G+G VL V EIQ R DA R++ER LLEL Q+FLDMAV+
Sbjct: 90 IDDLIETGRSEQIFKDAVQQQGRGQVLDTVAEIQERHDAVRDLERKLLELQQIFLDMAVL 269
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
VE QG+ ++ IE+HV+NA++++Q G L AK+ Q++SRK +C
Sbjct: 270 VEAQGDMINHIETHVSNATNHIQQGVGALQNAKKLQKNSRKWMC 401
>gb|AY246906.1| Hordeum vulgare subsp. vulgare syntaxin (Ror2) gene, complete cds
Length = 2346
Score = 104 bits (221), Expect(2) = 8e-027
Identities = 46/86 (53%), Positives = 59/86 (68%)
Frame = -1 / +1
Query: 611 AAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVMVETQGEKLDDIESHV 432
A + G+G VL V EIQ R A ++ERSLLEL QVF DMAV+V QGE+LDDIE HV
Sbjct: 1645 AIAEQQGRGEVLGVVAEIQERHGAVADLERSLLELQQVFNDMAVLVAAQGEQLDDIEGHV 1824
Query: 431 ANASHYVQGGNKELGKAKEYQRSSRK 354
A +V G ++L A+++Q+SSRK
Sbjct: 1825 GRARSFVDRGREQLQVARKHQKSSRK 1902
Score = 35.9 bits (72), Expect(2) = 8e-027
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -3 / +3
Query: 372 PEKQPQVSLHRHHNPAAPRPACHRANRHQPQE 277
PE+ PQV LHRH +PA P +R Q +
Sbjct: 1884 PEELPQVDLHRHRHPARRHPHHRHPHRAQEHQ 1979
>gb|AY246907.1| Hordeum vulgare subsp. vulgare syntaxin (Ror2) mRNA, complete cds
Length = 1293
Score = 104 bits (221), Expect(2) = 8e-027
Identities = 46/86 (53%), Positives = 59/86 (68%)
Frame = -1 / +2
Query: 611 AAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVMVETQGEKLDDIESHV 432
A + G+G VL V EIQ R A ++ERSLLEL QVF DMAV+V QGE+LDDIE HV
Sbjct: 764 AIAEQQGRGEVLGVVAEIQERHGAVADLERSLLELQQVFNDMAVLVAAQGEQLDDIEGHV 943
Query: 431 ANASHYVQGGNKELGKAKEYQRSSRK 354
A +V G ++L A+++Q+SSRK
Sbjct: 944 GRARSFVDRGREQLQVARKHQKSSRK 1021
Score = 35.9 bits (72), Expect(2) = 8e-027
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -3 / +1
Query: 372 PEKQPQVSLHRHHNPAAPRPACHRANRHQPQE 277
PE+ PQV LHRH +PA P +R Q +
Sbjct: 1003 PEELPQVDLHRHRHPARRHPHHRHPHRAQEHQ 1098
>gb|CB880180.1|CB880180 HM05L22w HM Hordeum vulgare subsp. vulgare cDNA clone HM05L22
3-PRIME, mRNA sequence
Length = 600
Score = 104 bits (221), Expect(2) = 8e-027
Identities = 46/86 (53%), Positives = 59/86 (68%)
Frame = -1 / -1
Query: 611 AAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVMVETQGEKLDDIESHV 432
A + G+G VL V EIQ R A ++ERSLLEL QVF DMAV+V QGE+LDDIE HV
Sbjct: 525 AIAEQQGRGEVLGVVAEIQERHGAVADLERSLLELQQVFNDMAVLVAAQGEQLDDIEGHV 346
Query: 431 ANASHYVQGGNKELGKAKEYQRSSRK 354
A +V G ++L A+++Q+SSRK
Sbjct: 345 GRARSFVDRGREQLQVARKHQKSSRK 268
Score = 35.9 bits (72), Expect(2) = 8e-027
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -3 / -3
Query: 372 PEKQPQVSLHRHHNPAAPRPACHRANRHQPQE 277
PE+ PQV LHRH +PA P +R Q +
Sbjct: 286 PEELPQVDLHRHRHPARRHPHHRHPHRAQEHQ 191
>gb|CB881364.1|CB881364 HM09J09w HM Hordeum vulgare subsp. vulgare cDNA clone HM09J09
3-PRIME, mRNA sequence
Length = 515
Score = 103 bits (219), Expect(2) = 1e-026
Identities = 45/82 (54%), Positives = 58/82 (70%)
Frame = -1 / -3
Query: 599 EHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVMVETQGEKLDDIESHVANAS 420
+ G+G VL V EIQ R A ++ERSLLEL QVF DMAV+V QGE+LDDIE HV A
Sbjct: 513 QQGRGEVLGVVAEIQERHGAVADLERSLLELQQVFNDMAVLVAAQGEQLDDIEGHVGRAR 334
Query: 419 HYVQGGNKELGKAKEYQRSSRK 354
+V G ++L A+++Q+SSRK
Sbjct: 333 SFVDRGREQLQVARKHQKSSRK 268
Score = 35.9 bits (72), Expect(2) = 1e-026
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -3 / -2
Query: 372 PEKQPQVSLHRHHNPAAPRPACHRANRHQPQE 277
PE+ PQV LHRH +PA P +R Q +
Sbjct: 286 PEELPQVDLHRHRHPARRHPHHRHPHRAQEHQ 191
>gb|AY247217.1| Hordeum vulgare subsp. vulgare syntaxin-like protein 4 gene,
partial cds
Length = 1352
Score = 119 bits (254), Expect = 2e-026
Identities = 50/85 (58%), Positives = 64/85 (75%)
Frame = -1 / +2
Query: 599 EHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVMVETQGEKLDDIESHVANAS 420
+ G+G V+A V EIQ R DA +++ERSLLELHQVFLDMA +VE QG +L++IE+HVA AS
Sbjct: 653 DQGRGEVMATVSEIQERHDAVKDIERSLLELHQVFLDMAALVEAQGHQLNNIETHVARAS 832
Query: 419 HYVQGGNKELGKAKEYQRSSRKCLC 345
+V G EL A+ YQ+SSRK C
Sbjct: 833 SFVVRGTVELESARVYQKSSRKWAC 907
>gb|AV919917.1|AV919917 AV919917 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags8f06 3', mRNA sequence
Length = 463
Score = 117 bits (251), Expect = 5e-026
Identities = 49/96 (51%), Positives = 70/96 (72%)
Frame = -1 / -2
Query: 632 RGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVMVETQGEKL 453
R E++ AV + G+G VL V EIQ R DA R++ER LLEL Q+FLDMAV+VE QG+ +
Sbjct: 399 RSEQIFKDAVQQQGRGQVLDTVXEIQERHDAVRDLERKLLELQQIFLDMAVLVEAQGDMI 220
Query: 452 DDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
+ IE+HV+NA++++Q G L AK+ Q++SRK +C
Sbjct: 219 NHIETHVSNATNHIQQGVGALQNAKKLQKNSRKXMC 112
>gb|CD662917.1|CD662917 UCRHV18_03ce02_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_03ce02, mRNA sequence
Length = 838
Score = 115 bits (246), Expect = 2e-025
Identities = 49/104 (47%), Positives = 68/104 (65%)
Frame = -1 / -3
Query: 656 IERIISDDRGEELLGAAVAEHGKGAVLAAVHEIQXRRDAAREVERSLLELHQVFLDMAVM 477
I+++I E++ A+ E G+G VL + EIQ R D +E+E LLEL Q+FLD++V+
Sbjct: 614 IDKLIETGDSEQIFERAIQEQGRGRVLDTLQEIQERHDTVKEIETKLLELQQIFLDLSVL 435
Query: 476 VETQGEKLDDIESHVANASHYVQGGNKELGKAKEYQRSSRKCLC 345
VETQGE LD+IE V A H+VQ G L KA+ Q++SRK C
Sbjct: 434 VETQGEILDNIEEQVTGAVHHVQTGTNLLQKARVLQKNSRKWTC 303
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 230,227,048
Number of Sequences: 312970
Number of extensions: 3325884
Number of successful extensions: 45127
Number of sequences better than 1.0e-020: 14
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7083590403
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)