TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750422.2.1
(1599 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ463879.1|BJ463879 BJ463879 K. Sato unpublished cDNA li... 462 e-129
gb|BJ463167.1|BJ463167 BJ463167 K. Sato unpublished cDNA li... 402 e-111
gb|CA025934.1|CA025934 HZ53J06r HZ Hordeum vulgare subsp. v... 292 3e-078
gb|BJ450389.1|BJ450389 BJ450389 K. Sato unpublished cDNA li... 241 4e-069
gb|BF254138.2|BF254138 HVSMEf0003C03f Hordeum vulgare seedl... 196 3e-049
gb|CA019139.1|CA019139 HV10O15r HV Hordeum vulgare subsp. v... 186 3e-046
gb|BJ468050.1|BJ468050 BJ468050 K. Sato unpublished cDNA li... 186 4e-046
gb|BJ466524.1|BJ466524 BJ466524 K. Sato unpublished cDNA li... 106 5e-032
gb|BJ463579.1|BJ463579 BJ463579 K. Sato unpublished cDNA li... 121 1e-026
gb|CK122942.1|CK122942 BES1824101g06 BES1824 Hordeum vulgar... 83 1e-025
gb|BJ473172.1|BJ473172 BJ473172 K. Sato unpublished cDNA li... 115 6e-025
gb|BQ465558.1|BQ465558 HU04A05r HU Hordeum vulgare subsp. v... 83 8e-025
gb|CA005298.1|CA005298 HU11C04u HU Hordeum vulgare subsp. v... 83 1e-022
gb|CB882152.1|CB882152 HL01A09w HL Hordeum vulgare subsp. v... 107 1e-022
gb|BG365307.1|BG365307 HVSMEi0002D20f Hordeum vulgare 20 DA... 55 4e-022
gb|BM816972.1|BM816972 HB107D11_SK.ab1 HB Hordeum vulgare s... 79 5e-022
gb|BQ464213.1|BQ464213 HF01K13T HF Hordeum vulgare subsp. v... 83 5e-022
gb|BU988066.1|BU988066 HF16J03r HF Hordeum vulgare subsp. v... 90 6e-022
gb|BQ768889.1|BQ768889 EBro08_SQ012_O23_R root, 3 week, dro... 91 2e-021
gb|AV909889.1|AV909889 AV909889 K. Sato unpublished cDNA li... 48 7e-021
>gb|BJ463879.1|BJ463879 BJ463879 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30n22 5', mRNA sequence
Length = 681
Score = 462 bits (1003), Expect = e-129
Identities = 182/225 (80%), Positives = 206/225 (91%)
Frame = -3 / +1
Query: 1267 ELSALGDKINKFSGMIEGLIWSPRGSDSNNQQDDWASEEGPHSDFAGPTCGSTACVALIR 1088
EL ALGDKIN+F+GMIEGLIWSPRGSDSN++ DDWA EEGPHSDF+GPTCGSTACVA++R
Sbjct: 4 ELQALGDKINQFTGMIEGLIWSPRGSDSNDRHDDWAFEEGPHSDFSGPTCGSTACVAMVR 183
Query: 1087 NTQLVVANAGDSRCVISRGGKAYNLSRDHKPELAAERERIMKAGGFIHMGRVNGSLNLSR 908
N+QLVVANAGDSRCVISR G+AYNLSRDHKPEL AERERI+KAGG+I MGRVNG++NLSR
Sbjct: 184 NSQLVVANAGDSRCVISRNGQAYNLSRDHKPELEAERERILKAGGYIQMGRVNGTINLSR 363
Query: 907 AIGDVELKQNKFLPPEKQIVTANPDINVVELCXXXXXXXVACDGIWDCMSSQQLVDFIHE 728
AIGD+E KQNKFL P+KQ++TANPD+N VELC +ACDGIWDCMSSQQLVDFIHE
Sbjct: 364 AIGDMEFKQNKFLSPDKQMLTANPDVNTVELCDDDDFLVLACDGIWDCMSSQQLVDFIHE 543
Query: 727 RINTESSLSAVCERVLDRCLAPSTIAGDGCDNMTMILVQFKKPVD 593
+ TESSLSAVCE+VLDRCLAPST+ G+GCDNMTMILVQFKKP+D
Sbjct: 544 HLKTESSLSAVCEKVLDRCLAPSTLGGEGCDNMTMILVQFKKPID 678
Score = 356 bits (771), Expect = 2e-097
Identities = 156/225 (69%), Positives = 166/225 (73%)
Frame = +3 / -2
Query: 594 STGFLNCTRIMVMLSHPSPAIVDGARHLSSTLSHTADRLLSVFIRSWIKSTSCWLDMQSQ 773
S GFLNCTRI+VMLSHPSP VDGARHLSST SHTA+RLLSVF SW+KSTSCWLDMQSQ
Sbjct: 677 SIGFLNCTRIIVMLSHPSPPNVDGARHLSSTFSHTAERLLSVFKCSWMKSTSCWLDMQSQ 498
Query: 774 MPXXXXXXXXXXXXXXXXXXXGLAVTICFSGGRNLFCFNSTSPIALDKFKLPFTRPIWMK 953
MP G AV IC SG +NLFC NS SPIALDKF +PFTRPIWM
Sbjct: 497 MPSHAKTRKSSSSQSSTVFTSGFAVNICLSGDKNLFCLNSISPIALDKFIVPFTRPIWMY 318
Query: 954 PPAFIIXXXXXXXXXXXXXDKLYAFPPLEITHRESPAFATTSWVFLINATHAVLPHVGPA 1133
PPAF I DKLYA+P LEIT RESPAFATTS +FL AT AVLP VGP
Sbjct: 317 PPAFSIRSLSASSSGLWSLDKLYAWPFLEITQRESPAFATTS*LFLTIATQAVLPQVGPL 138
Query: 1134 KSE*GPSSEAQSSC*LFESLPLGDQINPSIIPLNLFILSPSADSS 1268
KSE*GPSS+AQSSC FESLPLGDQINPSI+P+N FILSPSA SS
Sbjct: 137 KSE*GPSSKAQSSCLSFESLPLGDQINPSIMPVNWFILSPSACSS 3
Score = 297 bits (643), Expect = 1e-079
Identities = 126/226 (55%), Positives = 158/226 (69%)
Frame = +2 / -1
Query: 593 IDRFFELHQDHGHVVAPVSSDR*WSQASVQHSLAHGRQAALRVYTLVDKIHQLLA*HAVP 772
++ ELH+DH HVVA S+ *WSQAS++H H R+A L + +D+I QLLA HAVP
Sbjct: 678 VNWLLELHKDHRHVVASFST*C*WSQASIEHLFTHCRKATLCL*VFMDEIDQLLARHAVP 499
Query: 773 NAITCYNNKVVIIAELHDVDIRIGRHNLLLGRQEFVLFQFHVSNCP*QV*TSIYSSHMDE 952
NAITC N K++II +L V +R+ +LL+ RQEF+LF+FH+SNC *QV +SIYS H+D
Sbjct: 498 NAITC*NKKIIIITKLDSVYVRVCCQHLLIRRQEFILFKFHISNCS*QVYSSIYSPHLDV 319
Query: 953 APCFHYPFSLSCKLWFVVPR*IVCLPTP*NNTSGITSICHNKLGVSY*CYTRCTPTCWPS 1132
PCF YPFSL KLWFVV *IVCL P*+NT+GI+ +CHNKL + Y C T CTP WP+
Sbjct: 318 PPCFQYPFSLCFKLWFVVS**IVCLAIP*DNTAGISGVCHNKLTIPYHCNTGCTPAGWPA 139
Query: 1133 KVRVRXXXXXXXXXXXXXXTAPWRPNQSFYHTTKLVYFVSKCR*FS 1270
K+RVR TA RP+Q FYH +KLVYF+SKC *FS
Sbjct: 138 KIRVRSLLKSPIIMSII*ITASRRPDQPFYHASKLVYFISKCL*FS 1
Score = 272 bits (589), Expect = 3e-072
Identities = 137/223 (61%), Positives = 154/223 (69%)
Frame = -1 / +2
Query: 1269 ENYLHLETK*TSLVV**KD*FGLQGAVIQIINKMIGLQRRDLTLTLLGQHVGVQRV*H** 1090
ENY HLE K*TSL+ **K * GL+ AVIQ+I+ MIGL RRDLTL L GQ GVQ V *
Sbjct: 2 ENYRHLEIK*TSLLA**KG*SGLREAVIQMIDMMIGLLRRDLTLILAGQPAGVQPVLQW* 181
Query: 1089 ETPNLLWQMLVIPDVLFQGVGRHTIYRGTTNQSLQLREKG**KQGASSIWDE*MEV*TCQ 910
NLLWQ IP VL QG+ RHTIY+ TTNQSL+ RE G *KQG +S W E*ME+*TCQ
Sbjct: 182 GIVNLLWQTPEIPAVLSQGMARHTIYQETTNQSLKQRENGY*KQGGTSKWGE*MEL*TCQ 361
Query: 909 GQLETWN*NRTNSCLPRSKL*RPILISTSWSSAMMTTLLL*HVMAFGTACQANSWWILST 730
QLE WN NR NSCL SK *+ L T S MM L *HVMAFGTAC+ANSW I S
Sbjct: 362 EQLEIWNLNRINSCLLISKC*QQTLT*TLSSFVMMMIFLF*HVMAFGTACRANSWSISSM 541
Query: 729 SV*TRRAACLPCARECWTDAWLHQRSLETGATT*P*SWCSSKN 601
+ * +R A L C + C DAWLHQ +E ATT* *S CSS++
Sbjct: 542 NT*RQRVAFLQCVKRCSIDAWLHQH*VEKDATT*R*SLCSSRS 670
Score = 193 bits (416), Expect(2) = 3e-071
Identities = 90/143 (62%), Positives = 101/143 (70%)
Frame = -2 / +3
Query: 1268 RTICTWRQNKQV*WYDRRIDLVSKGQ*FK*STR*LGFRGGTSL*LCWANMWEYSVCSINK 1089
RT TWR NK V W+DRR+DLVS+ Q*FK* T *LGF GGTSL* AN+ EYS+C K
Sbjct: 3 RTTGTWR*NKPVYWHDRRVDLVSERQ*FK**T**LGF*GGTSL*F*RANLREYSLCCNGK 182
Query: 1088 KHPTCCGKCW*FPMCYFKGWEGIQFIEGPQTRACS*ERKDNESRGLHPYGTSKWKFKLVK 909
+ TCCGK FP+CY K W GIQFI+ PQTRA S ER D ESRG+HP G SKW +KLVK
Sbjct: 183 E*STCCGKRRRFPLCYLKEWPGIQFIKRPQTRA*SRERTDTESRGVHPNGASKWNYKLVK 362
Query: 908 GNWRRGIETEQIPASREANCDGQ 840
NWR GI TE I S AN D +
Sbjct: 363 SNWRYGI*TE*ILVS**ANVDSK 431
Score = 122 bits (261), Expect(2) = 5e-057
Identities = 62/108 (57%), Positives = 68/108 (62%)
Frame = +1 / -3
Query: 607 *TAPGSWSCCRTRLQRSLMEPGICPALSRTRQTGCSPCLYARG*NPPAVGLTCSPKCHHM 786
*TA GS SCCR L LMEPGI A T Q G S L G*N PAVG TCSPKCHHM
Sbjct: 664 *TAQGSSSCCRILLHLMLMEPGIYRAPFHTLQKGYSLSLSVHG*NRPAVGSTCSPKCHHM 485
Query: 787 LQQQSRHHRRAPRR*YQDWPSQFASREAGICSVSIPRLQLPLTSLNFH 930
L+Q++ HH +A + Q S FA +E I SV IP LQL LTSL FH
Sbjct: 484 LKQENHHHHKARQCLRQGLLSTFAYQETRIYSV*IPYLQLLLTSL*FH 341
Score = 120 bits (256), Expect(2) = 5e-057
Identities = 55/90 (61%), Positives = 63/90 (70%)
Frame = +1 / -3
Query: 1000 CGPSINCMPSHPLK*HIGNHQHLPQQVGCFLLMLHTLYSHMLAQQSQSEVPPLKPNHLVD 1179
CG INCMP H L+*H GN + LPQQV L + H LYS LA+ +QSEVPP KPNH V
Sbjct: 271 CGLLINCMPGHSLR*HSGNLRRLPQQVDYSLPLQHRLYSRRLAR*NQSEVPPQKPNHHVY 92
Query: 1180 YLNHCPLETKSILLSYH*TCLFCLQVQIVL 1269
+LNHC ET+S LLS *T LF LQV +VL
Sbjct: 91 HLNHCLSETRSTLLSCQ*TGLFYLQVPVVL 2
Score = 96.8 bits (205), Expect(2) = 3e-071
Identities = 41/62 (66%), Positives = 46/62 (74%)
Frame = -2 / +3
Query: 788 SM*WHLGLHVKPTAGGFYPRAYKHGEQPVCRVRESAGQMPGSINDRWRRVRQHDHDPGAV 609
SM*WHLGLHV+PTAG F+P K E P C V + A MPGSIN RWRR+RQHD DP AV
Sbjct: 483 SM*WHLGLHVEPTAGRFHP*TLKDRE*PFCSV*KGAR*MPGSINIRWRRMRQHDDDPCAV 662
Query: 608 QK 603
Q+
Sbjct: 663 QE 668
>gb|BJ463167.1|BJ463167 BJ463167 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags26b03 5', mRNA sequence
Length = 545
Score = 402 bits (872), Expect = e-111
Identities = 161/181 (88%), Positives = 172/181 (95%)
Frame = -3 / +3
Query: 1552 ENGENDRLKLGLSSMQGWRATMEDAHSALLDLDSETAFFGVFDGHGGRVVAKFCAKYLHG 1373
E+GEND+LK GLSSMQGWRA+MEDAHSALLDLD+ETAFFGVFDGHGGRVVAKFCAKYLH
Sbjct: 3 EDGENDKLKFGLSSMQGWRASMEDAHSALLDLDNETAFFGVFDGHGGRVVAKFCAKYLHS 182
Query: 1372 QVLKSEAYSTGDLGAAVHRAFFRMDEMMQGQRGWRELSALGDKINKFSGMIEGLIWSPRG 1193
QVLKSEAYS+GDLG AVH+AFFRMDEMM+GQRGWRELSALGDK+NKFSGMIEGLIWSPRG
Sbjct: 183 QVLKSEAYSSGDLGTAVHKAFFRMDEMMRGQRGWRELSALGDKMNKFSGMIEGLIWSPRG 362
Query: 1192 SDSNNQQDDWASEEGPHSDFAGPTCGSTACVALIRNTQLVVANAGDSRCVISRGGKAYNL 1013
SDS N +DDWA EEGPHSDF GPTCGSTACVAL+RN QLVVANAGDSRCVISR G+AYNL
Sbjct: 363 SDSKNVEDDWALEEGPHSDFDGPTCGSTACVALVRNNQLVVANAGDSRCVISRAGQAYNL 542
Query: 1012 S 1010
S
Sbjct: 543 S 545
Score = 311 bits (674), Expect = 6e-084
Identities = 140/172 (81%), Positives = 146/172 (84%)
Frame = +3 / -2
Query: 1011 DKLYAFPPLEITHRESPAFATTSWVFLINATHAVLPHVGPAKSE*GPSSEAQSSC*LFES 1190
DKLYA+P LEITHRESPAFATTSW+FL +ATHAVLPHVGP+KSE GPSS AQSS FES
Sbjct: 544 DKLYAWPALEITHRESPAFATTSWLFLTSATHAVLPHVGPSKSEWGPSSRAQSSSTFFES 365
Query: 1191 LPLGDQINPSIIPLNLFILSPSADSSRQPLCPCIISSILKKALWTAAPRSPVE*ASLLRT 1370
LPLGDQINPSI+PLNLFILSPSADSS QPLCP IISSILKKAL TA PRSP E ASLLRT
Sbjct: 364 LPLGDQINPSIMPLNLFILSPSADSSLQPLCPRIISSILKKAL*TAVPRSPEEYASLLRT 185
Query: 1371 *PCRYFAQNLAXXXXXXXXXXXXNAVSLSRSSNAE*ASSMVARHPCIDERPN 1526
* CRYFAQNLA NAVSLSRSSNAE*ASSM+ARHPCIDERPN
Sbjct: 184 *ECRYFAQNLATTLPPWPSKTPKNAVSLSRSSNAE*ASSMLARHPCIDERPN 29
Score = 283 bits (612), Expect = 2e-075
Identities = 125/177 (70%), Positives = 139/177 (78%)
Frame = -2 / +2
Query: 1541 K**T*VRSFIYARMASNHGRCSFSIARS**RDSILWCF*WSWRKSRCQILCKISAWSSPQ 1362
K**T + SFIYAR+A HGRCSFSIARS D ILWCF* RK CQILCKIS SS Q
Sbjct: 14 K**TQIWSFIYARVACKHGRCSFSIARSGQ*DGILWCF*RPRRKGGCQILCKISTLSSSQ 193
Query: 1361 KRSLFNW*PRRCCP*SFLQNG*NDARTERLARTICTWRQNKQV*WYDRRIDLVSKGQ*FK 1182
K S+F W*PR CC *SFL NG*NDARTERL RTICT R ++QV W+DRR+DLVSK Q*F+
Sbjct: 194 K*SIFFW*PRNCCS*SFL*NG*NDARTERLERTICTRR*DEQVQWHDRRVDLVSKRQ*FE 373
Query: 1181 *STR*LGFRGGTSL*LCWANMWEYSVCSINKKHPTCCGKCW*FPMCYFKGWEGIQFI 1011
R*LG GGT L*L WANMW+YS+CS +K+ PTC GKCW*FP+CYF GW GIQF+
Sbjct: 374 ECRR*LGS*GGTPL*L*WANMWKYSMCSTSKE*PTCSGKCW*FPVCYF*GWPGIQFV 544
Score = 277 bits (599), Expect = 1e-073
Identities = 127/180 (70%), Positives = 133/180 (73%)
Frame = +1 / -3
Query: 1015 NCMPSHPLK*HIGNHQHLPQQVGCFLLMLHTLYSHMLAQQSQSEVPPLKPNHLVDYLNHC 1194
NCMP P K*H GNHQHLP QVG LL+LH LY HMLA QSQS VPP +P+HL+ NHC
Sbjct: 540 NCMPGQP*K*HTGNHQHLPLQVGYSLLVLHMLYFHMLAHQSQSGVPPQEPSHLLHSSNHC 361
Query: 1195 PLETKSILLSYH*TCLFCLQVQIVLANLSVLASFHPF*RKLYGQQRLGHQLNRLRF*GLD 1374
LETKS LLS H*TC L VQIVL+NLSVLASFHPF*RKLY QQ LGHQ N L F* L+
Sbjct: 360 LLETKSTLLSCH*TCSSYLLVQIVLSNLSVLASFHPF*RKLYEQQFLGHQKNMLHF*ELE 181
Query: 1375 HADILHRIWQRLFLHDHQKHQRMLSRYQDLAMLNEHLPWLLAILA*MKDLT*VYHFLHFQ 1554
DILHRIWQ FL QKHQRM S DLAMLNEHLP L A LA*MKD *VYHF Q
Sbjct: 180 SVDILHRIWQPPFLRGRQKHQRMPSHCPDLAMLNEHLPCLHATLA*MKDQI*VYHFPRLQ 1
Score = 255 bits (552), Expect = 4e-067
Identities = 125/180 (69%), Positives = 136/180 (75%)
Frame = -1 / +1
Query: 1554 LKMEKMIDLS*VFHLCKDGEQPWKMLIQHC*ILIARQHSLVFLMVMEEESLPNSVQNICM 1375
LK KMI+ + VFHLCK G Q WKMLIQHC*I R+HSLVFL EE LPNSVQNI
Sbjct: 1 LKTGKMINSNLVFHLCKGGVQAWKMLIQHC*IWTMRRHSLVFLTATEEGWLPNSVQNIYT 180
Query: 1374 VKSSKAKPIQLVT*ALLSIELSSEWMK*CKDREVGENYLHLETK*TSLVV**KD*FGLQG 1195
+K SK K I LVT* LL I+LS EWMK*C+DREVGENYLH E +*TS V **K *FGLQ
Sbjct: 181 LKFSKVKHILLVT*ELLFIKLSLEWMK*CEDREVGENYLH*EIR*TSSVA**KG*FGLQE 360
Query: 1194 AVIQIINKMIGLQRRDLTLTLLGQHVGVQRV*H**ETPNLLWQMLVIPDVLFQGVGRHTI 1015
AVI+ + KM GL RRD TLTL+GQHV VQ V*H** NL WQMLVIP VLF G+ RHTI
Sbjct: 361 AVIRRM*KMTGLLRRDPTLTLMGQHVEVQHV*H**GITNL*WQMLVIPGVLFLGLARHTI 540
Score = 251 bits (543), Expect = 7e-066
Identities = 107/179 (59%), Positives = 122/179 (68%)
Frame = +2 / -1
Query: 1016 IVCLPTP*NNTSGITSICHNKLGVSY*CYTRCTPTCWPSKVRVRXXXXXXXXXXXXXXTA 1195
IVCL +P NNT GITSICH KL + Y*CYT CT TCWP KVRV TA
Sbjct: 539 IVCLASPRNNTPGITSICHYKLVIPY*CYTCCTSTCWPIKVRVGSLLKSPVIFYILRITA 360
Query: 1196 PWRPNQSFYHTTKLVYFVSKCR*FSPTSLSLHHFIHSEEXXXXXXXXXXXXIGFAFEDLT 1375
WRPNQ FYH T+LV+ +S CR*FSPTSLS HHFIHS+E I F FE+L
Sbjct: 359 SWRPNQPFYHATELVHLIS*CR*FSPTSLSSHHFIHSKESFMNSSS*VTRRICFTFENLR 180
Query: 1376 MQIFCTEFGNDSSSMTIKNTKECCLAIKI*QC*MSIFHGCSPSLHR*KT*LKSIIFSIF 1552
+ IFCTEFGN SS+ +KNTKEC L ++I*QC*MSIFH C+P LHR*KT + IIF +F
Sbjct: 179 V*IFCTEFGNHPSSVAVKNTKECRLIVQI*QC*MSIFHACTPPLHR*KTKFEFIIFPVF 3
>gb|CA025934.1|CA025934 HZ53J06r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ53J06
5-PRIME, mRNA sequence
Length = 445
Score = 292 bits (633), Expect = 3e-078
Identities = 116/147 (78%), Positives = 131/147 (89%)
Frame = -3 / +3
Query: 1060 GDSRCVISRGGKAYNLSRDHKPELAAERERIMKAGGFIHMGRVNGSLNLSRAIGDVELKQ 881
GDSRCVISR G+AYNLSRDHKPEL AERERI+KAGG+I MGRVNG++NLSRAIGD+E KQ
Sbjct: 3 GDSRCVISRNGQAYNLSRDHKPELEAERERILKAGGYIQMGRVNGTINLSRAIGDMEFKQ 182
Query: 880 NKFLPPEKQIVTANPDINVVELCXXXXXXXVACDGIWDCMSSQQLVDFIHERINTESSLS 701
NKFL P+KQ++TANPD+N VELC +ACDGIWDCMSSQQLVDFIHE + TESSLS
Sbjct: 183 NKFLSPDKQMLTANPDVNTVELCDDDDFLVLACDGIWDCMSSQQLVDFIHEHLKTESSLS 362
Query: 700 AVCERVLDRCLAPSTIAGDGCDNMTMI 620
AVCE+VLDRCLAPST+ G+GCDNMTMI
Sbjct: 363 AVCEKVLDRCLAPSTLGGEGCDNMTMI 443
Score = 221 bits (477), Expect = 9e-057
Identities = 94/148 (63%), Positives = 100/148 (67%)
Frame = +3 / -1
Query: 618 RIMVMLSHPSPAIVDGARHLSSTLSHTADRLLSVFIRSWIKSTSCWLDMQSQMPXXXXXX 797
RI+VMLSHPSP VDGARHLSST SHTA+RLLSVF SW+KSTSCWLDMQSQMP
Sbjct: 445 RIIVMLSHPSPPNVDGARHLSSTFSHTAERLLSVFKCSWMKSTSCWLDMQSQMPSHAKTR 266
Query: 798 XXXXXXXXXXXXXGLAVTICFSGGRNLFCFNSTSPIALDKFKLPFTRPIWMKPPAFIIXX 977
G AV IC SG +NLFC NS SPIALDKF +PFTRPIWM PPAF I
Sbjct: 265 KSSSSQSSTVFTSGFAVNICLSGDKNLFCLNSISPIALDKFIVPFTRPIWMYPPAFSIRS 86
Query: 978 XXXXXXXXXXXDKLYAFPPLEITHRESP 1061
DKLYA+P LEIT RESP
Sbjct: 85 LSASSSGLWSLDKLYAWPFLEITQRESP 2
Score = 200 bits (432), Expect = 1e-050
Identities = 87/147 (59%), Positives = 109/147 (74%)
Frame = +2 / -3
Query: 620 DHGHVVAPVSSDR*WSQASVQHSLAHGRQAALRVYTLVDKIHQLLA*HAVPNAITCYNNK 799
DH HVVA S+ *WSQAS++H H R+A L + +D+I QLLA HAVPNAITC N K
Sbjct: 443 DHRHVVASFST*C*WSQASIEHLFTHCRKATLCL*VFMDEIDQLLARHAVPNAITC*NKK 264
Query: 800 VVIIAELHDVDIRIGRHNLLLGRQEFVLFQFHVSNCP*QV*TSIYSSHMDEAPCFHYPFS 979
++II +L V +R+ +LL+ RQEF+LF+FH+SNC *QV +SIYS H+D PCF YPFS
Sbjct: 263 IIIITKLDSVYVRVCCQHLLIRRQEFILFKFHISNCS*QVYSSIYSPHLDVPPCFQYPFS 84
Query: 980 LSCKLWFVVPR*IVCLPTP*NNTSGIT 1060
L KLWFVV *IVCL P*+NT+GI+
Sbjct: 83 LCFKLWFVVS**IVCLAIP*DNTAGIS 3
Score = 184 bits (397), Expect = 9e-046
Identities = 89/146 (60%), Positives = 100/146 (68%)
Frame = -1 / +1
Query: 1056 IPDVLFQGVGRHTIYRGTTNQSLQLREKG**KQGASSIWDE*MEV*TCQGQLETWN*NRT 877
IP VL QG+ RHTIY+ TTNQSL+ RE G *KQG +S W E*ME+*TCQ QLE WN NR
Sbjct: 7 IPAVLSQGMARHTIYQETTNQSLKQRENGY*KQGGTSKWGE*MEL*TCQEQLEIWNLNRI 186
Query: 876 NSCLPRSKL*RPILISTSWSSAMMTTLLL*HVMAFGTACQANSWWILSTSV*TRRAACLP 697
NSCL SK *+ L T S MM L *HVMAFGTAC+ANSW I S + * +R A L
Sbjct: 187 NSCLLISKC*QQTLT*TLSSFVMMMIFLF*HVMAFGTACRANSWSISSMNT*RQRVAFLQ 366
Query: 696 CARECWTDAWLHQRSLETGATT*P*S 619
C + C DAWLHQ +E ATT* *S
Sbjct: 367 CVKRCSIDAWLHQH*VEKDATT*R*S 444
Score = 118 bits (252), Expect(2) = 9e-031
Identities = 59/104 (56%), Positives = 65/104 (62%)
Frame = +1 / -2
Query: 619 GSWSCCRTRLQRSLMEPGICPALSRTRQTGCSPCLYARG*NPPAVGLTCSPKCHHMLQQQ 798
GS SCCR L LMEPGI A T Q G S L G*N PAVG TCSPKCHHML+Q+
Sbjct: 444 GSSSCCRILLHLMLMEPGIYRAPFHTLQKGYSLSLSVHG*NRPAVGSTCSPKCHHMLKQE 265
Query: 799 SRHHRRAPRR*YQDWPSQFASREAGICSVSIPRLQLPLTSLNFH 930
+ HH +A + Q S FA +E I SV IP LQL LTSL FH
Sbjct: 264 NHHHHKARQCLRQGLLSTFAYQETRIYSV*IPYLQLLLTSL*FH 133
Score = 108 bits (231), Expect(2) = 2e-044
Identities = 46/72 (63%), Positives = 51/72 (70%)
Frame = -2 / +2
Query: 1055 FPMCYFKGWEGIQFIEGPQTRACS*ERKDNESRGLHPYGTSKWKFKLVKGNWRRGIETEQ 876
FP+CY K W GIQFI+ PQTRA S ER D ESRG+HP G SKW +KLVK NWR GI TE
Sbjct: 8 FPLCYLKEWPGIQFIKRPQTRA*SRERTDTESRGVHPNGASKWNYKLVKSNWRYGI*TE* 187
Query: 875 IPASREANCDGQ 840
I S AN D +
Sbjct: 188 ILVS**ANVDSK 223
Score = 91.8 bits (194), Expect(2) = 2e-044
Identities = 38/57 (66%), Positives = 42/57 (73%)
Frame = -2 / +2
Query: 788 SM*WHLGLHVKPTAGGFYPRAYKHGEQPVCRVRESAGQMPGSINDRWRRVRQHDHDP 618
SM*WHLGLHV+PTAG F+P K E P C V + A MPGSIN RWRR+RQHD DP
Sbjct: 275 SM*WHLGLHVEPTAGRFHP*TLKDRE*PFCSV*KGAR*MPGSINIRWRRMRQHDDDP 445
Score = 36.3 bits (73), Expect(2) = 9e-031
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = +1 / -2
Query: 1000 CGPSINCMPSHPLK*HIGN 1056
CG INCMP H L+*H GN
Sbjct: 63 CGLLINCMPGHSLR*HSGN 7
>gb|BJ450389.1|BJ450389 BJ450389 K. Sato unpublished cDNA library, cv. Akashinriki vegetative
stage leaves Hordeum vulgare subsp. vulgare cDNA clone
baak29f20 5', mRNA sequence
Length = 625
Score = 241 bits (521), Expect(2) = 4e-069
Identities = 98/141 (69%), Positives = 118/141 (83%)
Frame = -3 / +3
Query: 1078 LVVANAGDSRCVISRGGKAYNLSRDHKPELAAERERIMKAGGFIHMGRVNGSLNLSRAIG 899
LVVANAGDSRCVISR G+A++LSRD KPEL +E+ERI AGG+I GRVNGSLNL+RAIG
Sbjct: 9 LVVANAGDSRCVISRKGQAHDLSRDXKPELESEKERIQNAGGYIVAGRVNGSLNLTRAIG 188
Query: 898 DVELKQNKFLPPEKQIVTANPDINVVELCXXXXXXXVACDGIWDCMSSQQLVDFIHERIN 719
D+E+K+NK LP EKQ V+A P++N V L +ACDGIWDCMSSQQ+VDF+HE++N
Sbjct: 189 DMEMKENKLLPAEKQTVSAEPEVNTVTLSEDDEFIVLACDGIWDCMSSQQVVDFVHEKLN 368
Query: 718 TESSLSAVCERVLDRCLAPST 656
TE SLSAVCE+VLDRCLAP +
Sbjct: 369 TEESLSAVCEKVLDRCLAPES 431
Score = 154 bits (331), Expect(2) = 9e-040
Identities = 76/141 (53%), Positives = 83/141 (58%)
Frame = +3 / -1
Query: 657 VDGARHLSSTLSHTADRLLSVFIRSWIKSTSCWLDMQSQMPXXXXXXXXXXXXXXXXXXX 836
+ GA+ SST SHTADRL SVF S KST+C LDMQS +P
Sbjct: 430 LSGAKQRSSTFSHTADRLSSVFSFSCTKSTTC*LDMQSHIPSQAKTMNSSSSESVTVFTS 251
Query: 837 GLAVTICFSGGRNLFCFNSTSPIALDKFKLPFTRPIWMKPPAFIIXXXXXXXXXXXXXDK 1016
G A T+CFS G++LF F S SPIAL KFKLP TRP M PPAF I DK
Sbjct: 250 GSAETVCFSAGKSLFSFISISPIALVKFKLPLTRPATM*PPAF*ILSFSDSSSGLXSLDK 71
Query: 1017 LYAFPPLEITHRESPAFATTS 1079
A P LEITHRESPAFATTS
Sbjct: 70 S*A*PFLEITHRESPAFATTS 8
Score = 87.7 bits (185), Expect(3) = 6e-022
Identities = 42/88 (47%), Positives = 54/88 (61%)
Frame = +2 / -3
Query: 668 QASVQHSLAHGRQAALRVYTLVDKIHQLLA*HAVPNAITCYNNKVVIIAELHDVDIRIGR 847
QA+V+H LAH R L V L+ KIH LL *HAVP IT NN+ VI+ + + V R
Sbjct: 419 QATVEHFLAHSR*TFLGVQLLMHKIHYLLT*HAVPYTITGQNNEFVILRKCNCVHFRFS* 240
Query: 848 HNLLLGRQEFVLFQFHVSNCP*QV*TSI 931
+L LG Q+ + F H++NC QV TSI
Sbjct: 239 DSLFLGWQKLIFFHLHITNCSCQVQTSI 156
Score = 41.4 bits (84), Expect(2) = 4e-069
Identities = 19/55 (34%), Positives = 28/55 (50%)
Frame = -3 / +3
Query: 649 GDGCDNMTMILVQFKKPVDRNKKAEAAGQSASNVDEAKSRVAEE*SMAGTHQGAT 485
G+GCDNMTMILV KKP + + + + A + E+ ++ T AT
Sbjct: 435 GEGCDNMTMILVVLKKPTNSAATSSPEQSVVTPTNSAATSSPEQSAVTPTKSAAT 599
Score = 32.7 bits (65), Expect(3) = 6e-022
Identities = 13/21 (61%), Positives = 15/21 (71%)
Frame = +2 / -3
Query: 1016 IVCLPTP*NNTSGITSICHNK 1078
IV L P*+NT GI ICHN+
Sbjct: 71 IVSLTLP*DNTPGIPGICHNQ 9
Score = 30.4 bits (60), Expect(2) = 9e-040
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +3 / -1
Query: 600 GFLNCTRIMVMLSHPSPAI 656
GF + T+I+VMLS PSP +
Sbjct: 484 GFFSTTKIIVMLSQPSPPL 428
Score = 22.1 bits (42), Expect(3) = 6e-022
Identities = 8/15 (53%), Positives = 10/15 (66%)
Frame = +2 / -3
Query: 593 IDRFFELHQDHGHVV 637
I R + +QDH HVV
Sbjct: 491 ISRLLQHNQDHRHVV 447
>gb|BF254138.2|BF254138 HVSMEf0003C03f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0003C03f, mRNA sequence
Length = 529
Score = 196 bits (422), Expect = 3e-049
Identities = 81/107 (75%), Positives = 89/107 (83%)
Frame = -3 / +1
Query: 859 KQIVTANPDINVVELCXXXXXXXVACDGIWDCMSSQQLVDFIHERINTESSLSAVCERVL 680
KQIVT+NPDINVVELC +ACDGIWDCMSSQQLVDFIHE I+TES+LSAVCERVL
Sbjct: 1 KQIVTSNPDINVVELCNEDDFLVLACDGIWDCMSSQQLVDFIHEHIHTESTLSAVCERVL 180
Query: 679 DRCLAPSTIAGDGCDNMTMILVQFKKPVDRNKKAEAAGQSASNVDEA 539
DRCLAPST+ GDGCDNMTMILVQFKKPV K A+A QSA +E+
Sbjct: 181 DRCLAPSTMGGDGCDNMTMILVQFKKPVSDKKNADAGEQSAKGKEES 321
Score = 152 bits (327), Expect = 4e-036
Identities = 63/99 (63%), Positives = 69/99 (69%)
Frame = +3 / -3
Query: 561 DCPAASAFLFRSTGFLNCTRIMVMLSHPSPAIVDGARHLSSTLSHTADRLLSVFIRSWIK 740
DC ASAF TGFLNCT+IMVMLSHPSP IVDGARHLSSTLSHTA+R+LSV I SW+K
Sbjct: 299 DCSPASAFFLSLTGFLNCTKIMVMLSHPSPPIVDGARHLSSTLSHTAERVLSVCICSWMK 120
Query: 741 STSCWLDMQSQMPXXXXXXXXXXXXXXXXXXXGLAVTIC 857
ST+CWLD+QSQMP G VTIC
Sbjct: 119 STNCWLDIQSQMPSHAKTRKSSSLQSSTTLMSGFEVTIC 3
Score = 148 bits (318), Expect = 7e-035
Identities = 62/88 (70%), Positives = 72/88 (81%)
Frame = +2 / -2
Query: 596 DRFFELHQDHGHVVAPVSSDR*WSQASVQHSLAHGRQAALRVYTLVDKIHQLLA*HAVPN 775
D FFELHQDHGHVVA VS+ *WSQASV+HS H R+ A RVY LVD+IHQLLA*H VPN
Sbjct: 264 DWFFELHQDHGHVVASVSAHC*WSQASVEHSFTHCRKGAFRVYMLVDEIHQLLA*HTVPN 85
Query: 776 AITCYNNKVVIIAELHDVDIRIGRHNLL 859
AITC N KV++IA+LH++D+RI H LL
Sbjct: 84 AITC*NKKVILIAKLHNIDVRI*SHYLL 1
Score = 145 bits (311), Expect = 7e-034
Identities = 60/86 (69%), Positives = 66/86 (76%)
Frame = +1 / -1
Query: 601 VF*TAPGSWSCCRTRLQRSLMEPGICPALSRTRQTGCSPCLYARG*NPPAVGLTCSPKCH 780
VF*TAP SWSCCR RL+ LMEPGIC AL T Q GC PC+YARG*NPP VGLT SPKCH
Sbjct: 259 VF*TAPRSWSCCRIRLRPLLMEPGICRALFHTLQKGCFPCVYARG*NPPTVGLTYSPKCH 80
Query: 781 HMLQQQSRHHRRAPRR*YQDWPSQFA 858
HML+Q+S H +AP+ * QD S FA
Sbjct: 79 HMLKQESHPHCKAPQH*CQDLKSLFA 2
Score = 142 bits (304), Expect = 6e-033
Identities = 63/100 (63%), Positives = 70/100 (70%)
Frame = -1 / +2
Query: 858 SKL*RPILISTSWSSAMMTTLLL*HVMAFGTACQANSWWILSTSV*TRRAACLPCARECW 679
SK * IL S WS AM T L *HVMAFGT CQANSWWI STS+ TR+A L C +EC
Sbjct: 2 SK**LQILTSMLWSFAMRMTFLF*HVMAFGTVCQANSWWISSTSIYTRKAPFLQCVKECS 181
Query: 678 TDAWLHQRSLETGATT*P*SWCSSKNLSIGTRRPKQQGNL 559
TDAWLHQ+ ET ATT*P*SWCSSKN S+ + + NL
Sbjct: 182 TDAWLHQQWAETDATT*P*SWCSSKNQSVTRKMLTRGSNL 301
Score = 133 bits (285), Expect = 3e-030
Identities = 60/105 (57%), Positives = 74/105 (70%)
Frame = -2 / +3
Query: 857 ANCDGQS*YQRRGAXXXXXXXXCSM*WHLGLHVKPTAGGFYPRAYKHGEQPVCRVRESAG 678
AN D +S*+Q GA SM*WHLGL+VKPT GGF+PRAY HG+ P C V +SA
Sbjct: 3 ANSDFKS*HQCCGALQ*G*LSCFSM*WHLGLYVKPTVGGFHPRAYTHGKHPFCSV*KSAR 182
Query: 677 QMPGSINDRWRRVRQHDHDPGAVQKTCRSEQEGRSSRAICQQCGR 543
QMPGSIN+ RR+RQHDHD GAVQKT + +++ AIC++ GR
Sbjct: 183 QMPGSINNGRRRMRQHDHDLGAVQKTSQ*QEKC*RGGAICERQGR 317
>gb|CA019139.1|CA019139 HV10O15r HV Hordeum vulgare subsp. vulgare cDNA clone HV10O15
5-PRIME, mRNA sequence
Length = 538
Score = 186 bits (401), Expect = 3e-046
Identities = 75/82 (91%), Positives = 80/82 (97%)
Frame = -3 / +3
Query: 1576 TPKTDKLSENGENDRLKLGLSSMQGWRATMEDAHSALLDLDSETAFFGVFDGHGGRVVAK 1397
TPKTDKLSE+GEND+LK GLSSMQGWRA+MEDAHSALLDLD+ETAFFGVFDGHGGRVVAK
Sbjct: 288 TPKTDKLSEDGENDKLKFGLSSMQGWRASMEDAHSALLDLDNETAFFGVFDGHGGRVVAK 467
Query: 1396 FCAKYLHGQVLKSEAYSTGDLG 1331
FCAKYLH QVLKSEAYS+GDLG
Sbjct: 468 FCAKYLHSQVLKSEAYSSGDLG 533
Score = 120 bits (256), Expect = 3e-026
Identities = 50/77 (64%), Positives = 60/77 (77%)
Frame = +2 / -3
Query: 1349 IGFAFEDLTMQIFCTEFGNDSSSMTIKNTKECCLAIKI*QC*MSIFHGCSPSLHR*KT*L 1528
I F FE+L + IFCTEFGN SS+ +KNTKEC L ++I*QC*MSIFH C+P LHR*KT
Sbjct: 515 ICFTFENLRV*IFCTEFGNHPSSVAVKNTKECRLIVQI*QC*MSIFHACTPPLHR*KTKF 336
Query: 1529 KSIIFSIFR*LVSFWST 1579
+ IIF +FR*L+ WST
Sbjct: 335 EFIIFPVFR*LIGLWST 285
Score = 117 bits (250), Expect = 2e-025
Identities = 56/84 (66%), Positives = 58/84 (69%)
Frame = +1 / -2
Query: 1327 QRLGHQLNRLRF*GLDHADILHRIWQRLFLHDHQKHQRMLSRYQDLAMLNEHLPWLLAIL 1506
Q LGHQ N L F* L+ DILHRIWQ FL QKHQRM S DLAMLNEHLP L A L
Sbjct: 537 QFLGHQKNMLHF*ELESVDILHRIWQPPFLRGRQKHQRMPSHCPDLAMLNEHLPCLHATL 358
Query: 1507 A*MKDLT*VYHFLHFQIACQFLEY 1578
A*MKD *VYHF QIA + LEY
Sbjct: 357 A*MKDQI*VYHFPRLQIAYRSLEY 286
Score = 114 bits (244), Expect = 1e-024
Identities = 55/84 (65%), Positives = 60/84 (71%)
Frame = -1 / +1
Query: 1578 VLQKLTSYLKMEKMIDLS*VFHLCKDGEQPWKMLIQHC*ILIARQHSLVFLMVMEEESLP 1399
VLQ+ SYLK KMI+ + VFHLCK G Q WKMLIQHC*I R+HSLVFL EE LP
Sbjct: 286 VLQRPISYLKTGKMINSNLVFHLCKGGVQAWKMLIQHC*IWTMRRHSLVFLTATEEGWLP 465
Query: 1398 NSVQNICMVKSSKAKPIQLVT*AL 1327
NSVQNI +K SK K I LVT* L
Sbjct: 466 NSVQNIYTLKFSKVKHILLVT*EL 537
Score = 114 bits (244), Expect = 1e-024
Identities = 56/85 (65%), Positives = 62/85 (72%)
Frame = -2 / +2
Query: 1580 RYSKN*QAI*KWRK**T*VRSFIYARMASNHGRCSFSIARS**RDSILWCF*WSWRKSRC 1401
+YSK+ AI*+ K**T + SFIYAR+A HGRCSFSIARS D ILWCF* RK C
Sbjct: 284 QYSKDR*AI*RRGK**TQIWSFIYARVACKHGRCSFSIARSGQ*DGILWCF*RPRRKGGC 463
Query: 1400 QILCKISAWSSPQKRSLFNW*PRRC 1326
QILCKIS SS QK S+F W*PR C
Sbjct: 464 QILCKISTLSSSQK*SIFFW*PRNC 538
Score = 107 bits (229), Expect = 1e-022
Identities = 52/84 (61%), Positives = 53/84 (63%)
Frame = +3 / -1
Query: 1326 AAPRSPVE*ASLLRT*PCRYFAQNLAXXXXXXXXXXXXNAVSLSRSSNAE*ASSMVARHP 1505
A PRSP E ASLLRT* CRYFAQNLA NAVSLSRSSNAE*ASSM+ARHP
Sbjct: 538 AVPRSPEEYASLLRT*ECRYFAQNLATTLPPWPSKTPKNAVSLSRSSNAE*ASSMLARHP 359
Query: 1506 CIDERPNXXXXXXXXXXXXXXXGV 1577
CIDERPN GV
Sbjct: 358 CIDERPNLSLSFSPSSDSLSVFGV 287
>gb|BJ468050.1|BJ468050 BJ468050 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30n22 3', mRNA sequence
Length = 636
Score = 186 bits (400), Expect = 4e-046
Identities = 74/110 (67%), Positives = 87/110 (79%)
Frame = -3 / -3
Query: 862 EKQIVTANPDINVVELCXXXXXXXVACDGIWDCMSSQQLVDFIHERINTESSLSAVCERV 683
+KQ++TANPD+N VELC +ACDGIWDCMSSQQLVDFIHE + TESSLSAVCE+V
Sbjct: 634 DKQMLTANPDVNTVELCDDDDFLVLACDGIWDCMSSQQLVDFIHEHLKTESSLSAVCEKV 455
Query: 682 LDRCLAPSTIAGDGCDNMTMILVQFKKPVDRNKKAEAAGQSASNVDEAKS 533
LDRCLAPST+ G+GCDNMTMILVQFKKP+D K A A + A +A +
Sbjct: 454 LDRCLAPSTLGGEGCDNMTMILVQFKKPIDHGKNASAGEKLAVEDKDASA 305
Score = 144 bits (310), Expect = 9e-034
Identities = 63/97 (64%), Positives = 66/97 (68%)
Frame = +3 / +3
Query: 573 ASAFLFRSTGFLNCTRIMVMLSHPSPAIVDGARHLSSTLSHTADRLLSVFIRSWIKSTSC 752
A AFL S GFLNCTRI+VMLSHPSP VDGARHLSST SHTA+RLLSVF SW+KSTSC
Sbjct: 345 ALAFLPWSIGFLNCTRIIVMLSHPSPPNVDGARHLSSTFSHTAERLLSVFKCSWMKSTSC 524
Query: 753 WLDMQSQMPXXXXXXXXXXXXXXXXXXXGLAVTICFS 863
WLDMQSQMP G AV IC S
Sbjct: 525 WLDMQSQMPSHAKTRKSSSSQSSTVFTSGFAVNICLS 635
Score = 105 bits (225), Expect = 5e-022
Identities = 48/111 (43%), Positives = 70/111 (63%)
Frame = +2 / +2
Query: 530 TTLGFVHIAGRLPCCFGLLVPIDRFFELHQDHGHVVAPVSSDR*WSQASVQHSLAHGRQA 709
T+ G + + + G+ ++ ELH+DH HVVA S+ *WSQAS++H H R+A
Sbjct: 302 TSTGILILHSQFFTSTGIFAMVNWLLELHKDHRHVVASFST*C*WSQASIEHLFTHCRKA 481
Query: 710 ALRVYTLVDKIHQLLA*HAVPNAITCYNNKVVIIAELHDVDIRIGRHNLLL 862
L + +D+I QLLA HAVPNAITC N K++II +L V +R+ +LL+
Sbjct: 482 TLCL*VFMDEIDQLLARHAVPNAITC*NKKIIIITKLDSVYVRVCCQHLLI 634
>gb|BJ466524.1|BJ466524 BJ466524 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32j08 3', mRNA sequence
Length = 643
Score = 106 bits (226), Expect(2) = 5e-032
Identities = 44/59 (74%), Positives = 49/59 (83%)
Frame = -3 / -3
Query: 715 ESSLSAVCERVLDRCLAPSTIAGDGCDNMTMILVQFKKPVDRNKKAEAAGQSASNVDEA 539
ES+LSAVCERVLDRCLAPST+ GDGCDNMTMILVQFKKPV K A+A QSA +E+
Sbjct: 578 ESTLSAVCERVLDRCLAPSTMGGDGCDNMTMILVQFKKPVSDKKNADAGEQSAKGKEES 402
Score = 102 bits (217), Expect(2) = 2e-030
Identities = 42/52 (80%), Positives = 45/52 (86%)
Frame = +3 / +1
Query: 561 DCPAASAFLFRSTGFLNCTRIMVMLSHPSPAIVDGARHLSSTLSHTADRLLS 716
DC ASAF TGFLNCT+IMVMLSHPSP IVDGARHLSSTLSHTA+R+LS
Sbjct: 424 DCSPASAFFLSLTGFLNCTKIMVMLSHPSPPIVDGARHLSSTLSHTAERVLS 579
Score = 73.9 bits (155), Expect(2) = 5e-022
Identities = 32/58 (55%), Positives = 42/58 (72%)
Frame = -2 / -2
Query: 716 GEQPVCRVRESAGQMPGSINDRWRRVRQHDHDPGAVQKTCRSEQEGRSSRAICQQCGR 543
G+ P C V +SA QMPGSIN+ RR+RQHDHD GAVQKT + +++ AIC++ GR
Sbjct: 579 GKHPFCSV*KSARQMPGSINNGRRRMRQHDHDLGAVQKTSQ*QEKC*RGGAICERQGR 406
Score = 52.4 bits (108), Expect(2) = 5e-032
Identities = 18/24 (75%), Positives = 21/24 (87%)
Frame = -2 / -1
Query: 776 HLGLHVKPTAGGFYPRAYKHGEQP 705
HLGL+VKPT GGF+PRAY HG+ P
Sbjct: 640 HLGLYVKPTVGGFHPRAYTHGKAP 569
Score = 51.5 bits (106), Expect(2) = 2e-030
Identities = 19/24 (79%), Positives = 20/24 (83%)
Frame = +1 / +3
Query: 706 GCSPCLYARG*NPPAVGLTCSPKC 777
G PC+YARG*NPP VGLT SPKC
Sbjct: 570 GAFPCVYARG*NPPTVGLTYSPKC 641
Score = 51.5 bits (106), Expect(2) = 5e-022
Identities = 19/21 (90%), Positives = 20/21 (95%)
Frame = -3 / -2
Query: 778 GIWDCMSSQQLVDFIHERINT 716
GIWDCMSSQQLVDFIHE I+T
Sbjct: 642 GIWDCMSSQQLVDFIHEHIHT 580
>gb|BJ463579.1|BJ463579 BJ463579 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32j08 5', mRNA sequence
Length = 610
Score = 121 bits (259), Expect = 1e-026
Identities = 50/67 (74%), Positives = 56/67 (83%)
Frame = -3 / +3
Query: 739 FIHERINTESSLSAVCERVLDRCLAPSTIAGDGCDNMTMILVQFKKPVDRNKKAEAAGQS 560
FIHE I+TES+LSAVCERVLDRCLAPST+ GDGCDNMTMILVQFKKPV K A+A QS
Sbjct: 12 FIHEHIHTESTLSAVCERVLDRCLAPSTMGGDGCDNMTMILVQFKKPVSDKKNADAGEQS 191
Query: 559 ASNVDEA 539
A +E+
Sbjct: 192 AKGKEES 212
Score = 112 bits (239), Expect = 6e-024
Identities = 47/59 (79%), Positives = 50/59 (84%)
Frame = +3 / -1
Query: 561 DCPAASAFLFRSTGFLNCTRIMVMLSHPSPAIVDGARHLSSTLSHTADRLLSVFIRSWI 737
DC ASAF TGFLNCT+IMVMLSHPSP IVDGARHLSSTLSHTA+R+LSV I SWI
Sbjct: 190 DCSPASAFFLSLTGFLNCTKIMVMLSHPSPPIVDGARHLSSTLSHTAERVLSVCICSWI 14
>gb|CK122942.1|CK122942 BES1824101g06 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010G061 5-PRIME, mRNA sequence
Length = 774
Score = 83.1 bits (175), Expect(4) = 1e-025
Identities = 34/57 (59%), Positives = 44/57 (77%)
Frame = -3 / +1
Query: 1117 GSTACVALIRNTQLVVANAGDSRCVISRGGKAYNLSRDHKPELAAERERIMKAGGFI 947
GSTA A++ +LVVAN GDSR V+S+GGKA +SRDHKP+ ER+RI +AGGF+
Sbjct: 304 GSTASTAILVGGRLVVANVGDSRAVVSKGGKAIAVSRDHKPDQTDERQRIEEAGGFV 474
Score = 38.6 bits (78), Expect(4) = 1e-025
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -3 / +1
Query: 1438 FGVFDGHGGRVVAKFCAKYLHGQVLKSEAYST 1343
FGVFDGHGG A++ K+L ++K + T
Sbjct: 124 FGVFDGHGGARAAEYVKKHLFSNLIKHPKFMT 219
Score = 29.9 bits (59), Expect(4) = 1e-025
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = -3 / +1
Query: 859 KQIVTANPDINVVELCXXXXXXXVACDGIWDCMSSQQLVDFI 734
KQ V A+P+I + +A DG+WD +++ + V +
Sbjct: 544 KQYVVADPEIKEEVVDSSLEFLILASDGLWDVVTNDEAVAMV 669
Score = 28.1 bits (55), Expect(4) = 1e-025
Identities = 14/26 (53%), Positives = 15/26 (57%)
Frame = -3 / +1
Query: 937 RVNGSLNLSRAIGDVELKQNKFLPPE 860
RV G L +SRA GD LKQ PE
Sbjct: 493 RVGGVLAVSRAFGDKLLKQYVVADPE 570
>gb|BJ473172.1|BJ473172 BJ473172 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal38o09 5', mRNA sequence
Length = 599
Score = 115 bits (246), Expect = 6e-025
Identities = 51/150 (34%), Positives = 82/150 (54%)
Frame = -3 / +2
Query: 1117 GSTACVALIRNTQLVVANAGDSRCVISRGGKAYNLSRDHKPELAAERERIMKAGGFIHMG 938
G+ A A+I L+VANAGD R V+SR G A +S+DH+ ER+R+ GG++ G
Sbjct: 5 GTRALTAMIFGRSLLVANAGDCRAVLSRRGAAIEMSKDHRACCLNERKRVESLGGYVDDG 184
Query: 937 RVNGSLNLSRAIGDVELKQNKFLPPEKQIVTANPDINVVELCXXXXXXXVACDGIWDCMS 758
+NG L ++RA+GD L K + ++A P++ ++ L + DGIWD S
Sbjct: 185 YLNGQLAVTRALGDWHLDGLKEMGEPGGPLSAEPELKMITLTKDDEFLIIGSDGIWDFFS 364
Query: 757 SQQLVDFIHERINTESSLSAVCERVLDRCL 668
+Q VDF R+ + L C+ +++ +
Sbjct: 365 NQNSVDFARRRLQEHNDLRRCCKEIVEEAI 454
>gb|BQ465558.1|BQ465558 HU04A05r HU Hordeum vulgare subsp. vulgare cDNA clone HU04A05
5-PRIME, mRNA sequence
Length = 660
Score = 83.1 bits (175), Expect(4) = 8e-025
Identities = 34/57 (59%), Positives = 44/57 (77%)
Frame = -3 / +3
Query: 1117 GSTACVALIRNTQLVVANAGDSRCVISRGGKAYNLSRDHKPELAAERERIMKAGGFI 947
GSTA A++ +LVVAN GDSR V+S+GGKA +SRDHKP+ ER+RI +AGGF+
Sbjct: 324 GSTASTAILVGGRLVVANVGDSRAVVSKGGKAIAVSRDHKPDQTDERQRIEEAGGFV 494
Score = 38.6 bits (78), Expect(4) = 8e-025
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -3 / +3
Query: 1438 FGVFDGHGGRVVAKFCAKYLHGQVLKSEAYST 1343
FGVFDGHGG A++ K+L ++K + T
Sbjct: 144 FGVFDGHGGARAAEYVKKHLFSNLIKHPKFMT 239
Score = 28.1 bits (55), Expect(4) = 8e-025
Identities = 14/26 (53%), Positives = 15/26 (57%)
Frame = -3 / +3
Query: 937 RVNGSLNLSRAIGDVELKQNKFLPPE 860
RV G L +SRA GD LKQ PE
Sbjct: 513 RVGGVLAVSRAFGDKLLKQYVVADPE 590
Score = 26.3 bits (51), Expect(4) = 8e-025
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = -3 / +3
Query: 859 KQIVTANPDINVVELCXXXXXXXVACDGIWD 767
KQ V A+P+I + +A DG+WD
Sbjct: 564 KQYVVADPEIKEEVVDSSLEFLILASDGLWD 656
>gb|CA005298.1|CA005298 HU11C04u HU Hordeum vulgare subsp. vulgare cDNA clone HU11C04
3-PRIME, mRNA sequence
Length = 567
Score = 83.1 bits (175), Expect(3) = 1e-022
Identities = 34/57 (59%), Positives = 44/57 (77%)
Frame = -3 / +1
Query: 1117 GSTACVALIRNTQLVVANAGDSRCVISRGGKAYNLSRDHKPELAAERERIMKAGGFI 947
GSTA A++ +LVVAN GDSR V+S+GGKA +SRDHKP+ ER+RI +AGGF+
Sbjct: 265 GSTASTAILVGGRLVVANVGDSRAVVSKGGKAIAVSRDHKPDQTDERQRIEEAGGFV 435
Score = 38.6 bits (78), Expect(3) = 1e-022
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -3 / +1
Query: 1438 FGVFDGHGGRVVAKFCAKYLHGQVLKSEAYST 1343
FGVFDGHGG A++ K+L ++K + T
Sbjct: 85 FGVFDGHGGARAAEYVKKHLFSNLIKHPKFMT 180
Score = 28.1 bits (55), Expect(3) = 1e-022
Identities = 14/26 (53%), Positives = 15/26 (57%)
Frame = -3 / +1
Query: 937 RVNGSLNLSRAIGDVELKQNKFLPPE 860
RV G L +SRA GD LKQ PE
Sbjct: 454 RVGGVLAVSRAFGDKLLKQYVVADPE 531
>gb|CB882152.1|CB882152 HL01A09w HL Hordeum vulgare subsp. vulgare cDNA clone HL01A09
3-PRIME, mRNA sequence
Length = 418
Score = 107 bits (229), Expect = 1e-022
Identities = 43/48 (89%), Positives = 47/48 (97%)
Frame = -3 / +1
Query: 1576 TPKTDKLSENGENDRLKLGLSSMQGWRATMEDAHSALLDLDSETAFFG 1433
TPKTDKLSE+GEND+LK GLSSMQGWRA+MEDAHSALLDLD+ETAFFG
Sbjct: 274 TPKTDKLSEDGENDKLKFGLSSMQGWRASMEDAHSALLDLDNETAFFG 417
>gb|BG365307.1|BG365307 HVSMEi0002D20f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0002D20f, mRNA sequence
Length = 812
Score = 55.1 bits (114), Expect(5) = 4e-022
Identities = 24/41 (58%), Positives = 28/41 (68%)
Frame = -3 / +2
Query: 1117 GSTACVALIRNTQLVVANAGDSRCVISRGGKAYNLSRDHKP 995
G+TA ALI L+VANAGD R V+ GKA +SRDHKP
Sbjct: 290 GTTALAALIIGRSLLVANAGDCRAVVCCRGKAIEMSRDHKP 412
Score = 40.5 bits (82), Expect(5) = 4e-022
Identities = 16/36 (44%), Positives = 26/36 (72%)
Frame = -3 / +3
Query: 982 ERERIMKAGGFIHMGRVNGSLNLSRAIGDVELKQNK 875
E+ RI +GG+++ G +NG LN++RAIGD ++ K
Sbjct: 426 EKMRIEASGGYVYDGYLNGLLNVARAIGDWHMEGMK 533
Score = 35.9 bits (72), Expect(5) = 4e-022
Identities = 12/36 (33%), Positives = 22/36 (61%)
Frame = -3 / +2
Query: 1456 DSETAFFGVFDGHGGRVVAKFCAKYLHGQVLKSEAY 1349
+ ++F+GVFDGHGG+ A F L +++ + +
Sbjct: 89 EGPSSFYGVFDGHGGKHAADFVCSNLPRFIVEGDGF 196
Score = 29.5 bits (58), Expect(5) = 4e-022
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = -3 / +3
Query: 850 VTANPDINVVELCXXXXXXXVACDGIWDCMSSQ 752
++A P++ + L + CDGIWD SQ
Sbjct: 555 LSAEPEVMMRNLTEEDEFLIIGCDGIWDVFRSQ 653
Score = 24.4 bits (47), Expect(5) = 4e-022
Identities = 8/33 (24%), Positives = 15/33 (45%)
Frame = -3 / +2
Query: 766 CMSSQQLVDFIHERINTESSLSAVCERVLDRCL 668
C+S VDF + + A C+ ++D +
Sbjct: 638 CISQPNAVDFARRELQEHNDPIACCKELVDEAI 736
>gb|BM816972.1|BM816972 HB107D11_SK.ab1 HB Hordeum vulgare subsp. vulgare cDNA clone
HB107D11_SK.ab1 similar to (AC022314) F9C16.6
[Arabidopsis thaliana], mRNA sequence
Length = 870
Score = 79.4 bits (167), Expect(4) = 5e-022
Identities = 34/57 (59%), Positives = 41/57 (71%)
Frame = -3 / +3
Query: 1117 GSTACVALIRNTQLVVANAGDSRCVISRGGKAYNLSRDHKPELAAERERIMKAGGFI 947
GSTA A++ +LVVAN GDSR VI RGG A +SRDHKP+ ER+RI AGGF+
Sbjct: 393 GSTASTAILVGDRLVVANVGDSRAVICRGGDAIAVSRDHKPDQTDERQRIEDAGGFV 563
Score = 35.9 bits (72), Expect(4) = 5e-022
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = -3 / +3
Query: 1438 FGVFDGHGGRVVAKFCAKYLHGQVLKSEAYST 1343
FGVFDGHGG A+F + L ++K + T
Sbjct: 213 FGVFDGHGGARAAEFVKQNLFSNLIKHPKFFT 308
Score = 28.1 bits (55), Expect(4) = 5e-022
Identities = 14/26 (53%), Positives = 15/26 (57%)
Frame = -3 / +3
Query: 937 RVNGSLNLSRAIGDVELKQNKFLPPE 860
RV G L +SRA GD LKQ PE
Sbjct: 582 RVGGVLAVSRAFGDKLLKQYVVADPE 659
Score = 24.4 bits (47), Expect(4) = 5e-022
Identities = 6/27 (22%), Positives = 18/27 (66%)
Frame = -3 / +1
Query: 787 ACDGIWDCMSSQQLVDFIHERINTESS 707
A DG+WD +++++ V + ++++ +
Sbjct: 706 ASDGLWDVVTNEEAVAMVKPIVDSQQA 786
>gb|BQ464213.1|BQ464213 HF01K13T HF Hordeum vulgare subsp. vulgare cDNA clone HF01K13
5-PRIME, mRNA sequence
Length = 551
Score = 83.1 bits (175), Expect(3) = 5e-022
Identities = 34/57 (59%), Positives = 44/57 (77%)
Frame = -3 / +3
Query: 1117 GSTACVALIRNTQLVVANAGDSRCVISRGGKAYNLSRDHKPELAAERERIMKAGGFI 947
GSTA A++ +LVVAN GDSR V+S+GGKA +SRDHKP+ ER+RI +AGGF+
Sbjct: 291 GSTASTAILVGGRLVVANVGDSRAVVSKGGKAIAVSRDHKPDQTDERQRIEEAGGFV 461
Score = 38.6 bits (78), Expect(3) = 5e-022
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -3 / +3
Query: 1438 FGVFDGHGGRVVAKFCAKYLHGQVLKSEAYST 1343
FGVFDGHGG A++ K+L ++K + T
Sbjct: 111 FGVFDGHGGARAAEYVKKHLFSNLIKHPKFMT 206
Score = 25.8 bits (50), Expect(3) = 5e-022
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = -3 / +3
Query: 937 RVNGSLNLSRAIGDVELKQ 881
RV G L +SRA GD LKQ
Sbjct: 480 RVGGVLAVSRAFGDKLLKQ 536
>gb|BU988066.1|BU988066 HF16J03r HF Hordeum vulgare subsp. vulgare cDNA clone HF16J03
5-PRIME, mRNA sequence
Length = 594
Score = 90.0 bits (190), Expect(3) = 6e-022
Identities = 39/81 (48%), Positives = 54/81 (66%)
Frame = -3 / +3
Query: 1117 GSTACVALIRNTQLVVANAGDSRCVISRGGKAYNLSRDHKPELAAERERIMKAGGFIHMG 938
G+TA ALI L+VANAGD R V+ + G+A LS+DHKP +E+ RI GG + G
Sbjct: 189 GTTALTALIFGRTLLVANAGDCRAVLGKRGRAVELSKDHKPSCKSEKLRIENLGGIVFDG 368
Query: 937 RVNGSLNLSRAIGDVELKQNK 875
+NG L+++RAIGD +K +K
Sbjct: 369 YLNGQLSVARAIGDWHVKGSK 431
Score = 29.5 bits (58), Expect(3) = 6e-022
Identities = 12/43 (27%), Positives = 20/43 (46%)
Frame = -3 / +3
Query: 850 VTANPDINVVELCXXXXXXXVACDGIWDCMSSQQLVDFIHERI 722
+T P+ V L + CDG+ D M+SQ V + + +
Sbjct: 447 LTPEPEFQEVRLTEEDEFLIIGCDGLXDVMTSQCAVSMVRKEL 575
Score = 28.1 bits (55), Expect(3) = 6e-022
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = -3 / +3
Query: 1438 FGVFDGHGGRVVAKFCAKYL 1379
+GVFDGHGG F K L
Sbjct: 12 YGVFDGHGGTDAVCFVRKNL 71
>gb|BQ768889.1|BQ768889 EBro08_SQ012_O23_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ012_O23 5', mRNA sequence
Length = 670
Score = 90.9 bits (192), Expect(2) = 2e-021
Identities = 40/81 (49%), Positives = 54/81 (66%)
Frame = -3 / +3
Query: 1117 GSTACVALIRNTQLVVANAGDSRCVISRGGKAYNLSRDHKPELAAERERIMKAGGFIHMG 938
G+TA ALI L+VANAGD R V+ GKA +SRDHKP E+ RI +GG+++ G
Sbjct: 399 GTTALAALIIGRSLLVANAGDCRAVVCCRGKAIEMSRDHKPSCNREKMRIEASGGYVYDG 578
Query: 937 RVNGSLNLSRAIGDVELKQNK 875
+NG LN++RAIGD ++ K
Sbjct: 579 YLNGLLNVARAIGDWHMEGMK 641
Score = 35.9 bits (72), Expect(2) = 2e-021
Identities = 12/36 (33%), Positives = 22/36 (61%)
Frame = -3 / +3
Query: 1456 DSETAFFGVFDGHGGRVVAKFCAKYLHGQVLKSEAY 1349
+ ++F+GVFDGHGG+ A F L +++ + +
Sbjct: 198 EGPSSFYGVFDGHGGKHAADFVCSNLPRFIVEGDGF 305
>gb|AV909889.1|AV909889 AV909889 K. Sato unpublished cDNA library, cv. Akashinriki vegetative
stage leaves Hordeum vulgare subsp. vulgare cDNA clone
baak2f12 5', mRNA sequence
Length = 607
Score = 47.8 bits (98), Expect(3) = 7e-021
Identities = 20/41 (48%), Positives = 28/41 (68%)
Frame = -3 / +3
Query: 1117 GSTACVALIRNTQLVVANAGDSRCVISRGGKAYNLSRDHKP 995
G+TA +RN LVV++ GDS VISRGG+ +L+ H+P
Sbjct: 93 GATATALFLRNDVLVVSHIGDSCLVISRGGRPQSLTNFHRP 215
Score = 46.0 bits (94), Expect(3) = 7e-021
Identities = 18/36 (50%), Positives = 25/36 (69%)
Frame = -3 / +3
Query: 982 ERERIMKAGGFIHMGRVNGSLNLSRAIGDVELKQNK 875
E +RI AGG+IH GR+ G +++SRA GD+ K K
Sbjct: 243 EVKRIRAAGGWIHDGRICGDISVSRAFGDIRFKTRK 350
Score = 45.1 bits (92), Expect(3) = 7e-021
Identities = 16/55 (29%), Positives = 31/55 (56%)
Frame = -3 / +3
Query: 853 IVTANPDINVVELCXXXXXXXVACDGIWDCMSSQQLVDFIHERINTESSLSAVCE 689
++ ++PD+++VEL VA DG+WD + S + V F+ +++ + CE
Sbjct: 429 LIISSPDVSLVELGPDAEFVLVATDGLWDYIKSTEAVAFVRDQLRQHGDVQRACE 593
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 846,814,385
Number of Sequences: 312970
Number of extensions: 14803922
Number of successful extensions: 82199
Number of sequences better than 1.0e-020: 24
length of database: 58,378,179
effective HSP length: 54
effective length of database: 41,477,799
effective search space used: 19826387922
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)