TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2647051.2.1
(627 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF266997.3|BF266997 HV_CEa0016K10f Hordeum vulgare seedl... 160 5e-039
gb|BU995376.1|BU995376 HM10B01r HM Hordeum vulgare subsp. v... 160 5e-039
gb|CB881502.1|CB881502 HM10A02w HM Hordeum vulgare subsp. v... 160 5e-039
gb|CX662506.1|CX662506 EST 322 Expressed sequences from Bar... 160 5e-039
gb|CX662646.1|CX662646 EST 477 Expressed sequences from Bar... 160 5e-039
gb|AF474923.1| Hordeum vulgare subsp. vulgare chalcone isom... 160 5e-039
gb|BI957123.1|BI957123 HVSMEn0007J13f Hordeum vulgare rachi... 158 2e-038
gb|BI956899.1|BI956899 HVSMEn0005O19f Hordeum vulgare rachi... 121 3e-027
gb|CA024561.1|CA024561 HZ49K12r HZ Hordeum vulgare subsp. v... 110 9e-024
gb|BE195609.3|BE195609 HVSMEh0089O15f Hordeum vulgare 5-45 ... 105 2e-022
>gb|BF266997.3|BF266997 HV_CEa0016K10f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0016K10f, mRNA sequence
Length = 751
Score = 160 bits (345), Expect = 5e-039
Identities = 71/86 (82%), Positives = 76/86 (88%)
Frame = +2 / +3
Query: 2 EGVAVEKFREVFKPETFAPGASILFTHSTAGVLTVAFSKDSSVPAAGGVAIENKRLCEAV 181
E AV+KF+E F P +FAPGASILFTHS AGVLTVAFSKDSSVP +GGVAIEN RLCEAV
Sbjct: 321 EAAAVDKFKEAFGPHSFAPGASILFTHSPAGVLTVAFSKDSSVPESGGVAIENARLCEAV 500
Query: 182 LESIIGERGVSPAAKLSLAARVSELL 259
LESIIGE GVSPAAKLSLA RV+ELL
Sbjct: 501 LESIIGEHGVSPAAKLSLANRVAELL 578
Score = 135 bits (290), Expect = 5e-031
Identities = 62/82 (75%), Positives = 65/82 (79%)
Frame = -1 / -1
Query: 246 TLAARLSFAAGDTPRSPMMDSSTASQRRLFSIATPPAAGTDESLEKATVRTPAVECVKRM 67
TL ARLS AAGDTP SPMMDSSTASQ FS+ATPP +GTDESLEKATV TPA ECVK M
Sbjct: 565 TLLARLSLAAGDTPCSPMMDSSTASQSLAFSMATPPDSGTDESLEKATVSTPAGECVKSM 386
Query: 66 EAPGAKVSGLNTSLNFSTATPS 1
EAPGAK+ G SLN STA S
Sbjct: 385 EAPGAKLWGPKASLNLSTAAAS 320
Score = 113 bits (242), Expect = 7e-025
Identities = 53/81 (65%), Positives = 58/81 (71%)
Frame = -3 / -3
Query: 244 PRGEAQLRGRRHAALPDDGLQHGLAEALVLDRHAAGRRHRRVLGEGDGEDSGGRVREEDG 65
P G+A+L RRH L DDGLQHGLAE VLD HAAG RHRRVLGEGDGE +GG VREE G
Sbjct: 563 PVGKAELGRRRHTVLADDGLQHGLAEPGVLDGHAAGLRHRRVLGEGDGEHAGGGVREEYG 384
Query: 64 GARRESLWLEHLPELFYGDAL 2
GA E++ E L EL G L
Sbjct: 383 GAGGEAVGPEGLLELVDGGGL 321
>gb|BU995376.1|BU995376 HM10B01r HM Hordeum vulgare subsp. vulgare cDNA clone HM10B01
5-PRIME, mRNA sequence
Length = 602
Score = 160 bits (345), Expect = 5e-039
Identities = 71/86 (82%), Positives = 76/86 (88%)
Frame = +2 / +3
Query: 2 EGVAVEKFREVFKPETFAPGASILFTHSTAGVLTVAFSKDSSVPAAGGVAIENKRLCEAV 181
E AV+KF+E F P +FAPGASILFTHS AGVLTVAFSKDSSVP +GGVAIEN RLCEAV
Sbjct: 225 EAAAVDKFKEAFGPHSFAPGASILFTHSPAGVLTVAFSKDSSVPESGGVAIENARLCEAV 404
Query: 182 LESIIGERGVSPAAKLSLAARVSELL 259
LESIIGE GVSPAAKLSLA RV+ELL
Sbjct: 405 LESIIGEHGVSPAAKLSLANRVAELL 482
Score = 135 bits (290), Expect = 5e-031
Identities = 62/82 (75%), Positives = 65/82 (79%)
Frame = -1 / -2
Query: 246 TLAARLSFAAGDTPRSPMMDSSTASQRRLFSIATPPAAGTDESLEKATVRTPAVECVKRM 67
TL ARLS AAGDTP SPMMDSSTASQ FS+ATPP +GTDESLEKATV TPA ECVK M
Sbjct: 469 TLLARLSLAAGDTPCSPMMDSSTASQSLAFSMATPPDSGTDESLEKATVSTPAGECVKSM 290
Query: 66 EAPGAKVSGLNTSLNFSTATPS 1
EAPGAK+ G SLN STA S
Sbjct: 289 EAPGAKLWGPKASLNLSTAAAS 224
Score = 113 bits (242), Expect = 7e-025
Identities = 53/81 (65%), Positives = 58/81 (71%)
Frame = -3 / -1
Query: 244 PRGEAQLRGRRHAALPDDGLQHGLAEALVLDRHAAGRRHRRVLGEGDGEDSGGRVREEDG 65
P G+A+L RRH L DDGLQHGLAE VLD HAAG RHRRVLGEGDGE +GG VREE G
Sbjct: 467 PVGQAELGRRRHPVLADDGLQHGLAEPGVLDGHAAGLRHRRVLGEGDGEHAGGGVREEYG 288
Query: 64 GARRESLWLEHLPELFYGDAL 2
GA E++ E L EL G L
Sbjct: 287 GAGGEAVGPEGLLELVDGGGL 225
>gb|CB881502.1|CB881502 HM10A02w HM Hordeum vulgare subsp. vulgare cDNA clone HM10A02
3-PRIME, mRNA sequence
Length = 630
Score = 160 bits (345), Expect = 5e-039
Identities = 71/86 (82%), Positives = 76/86 (88%)
Frame = +2 / -2
Query: 2 EGVAVEKFREVFKPETFAPGASILFTHSTAGVLTVAFSKDSSVPAAGGVAIENKRLCEAV 181
E AV+KF+E F P +FAPGASILFTHS AGVLTVAFSKDSSVP +GGVAIEN RLCEAV
Sbjct: 488 EAAAVDKFKEAFGPHSFAPGASILFTHSPAGVLTVAFSKDSSVPESGGVAIENARLCEAV 309
Query: 182 LESIIGERGVSPAAKLSLAARVSELL 259
LESIIGE GVSPAAKLSLA RV+ELL
Sbjct: 308 LESIIGEHGVSPAAKLSLANRVAELL 231
Score = 135 bits (290), Expect = 5e-031
Identities = 62/82 (75%), Positives = 65/82 (79%)
Frame = -1 / +1
Query: 246 TLAARLSFAAGDTPRSPMMDSSTASQRRLFSIATPPAAGTDESLEKATVRTPAVECVKRM 67
TL ARLS AAGDTP SPMMDSSTASQ FS+ATPP +GTDESLEKATV TPA ECVK M
Sbjct: 244 TLLARLSLAAGDTPCSPMMDSSTASQSLAFSMATPPDSGTDESLEKATVSTPAGECVKSM 423
Query: 66 EAPGAKVSGLNTSLNFSTATPS 1
EAPGAK+ G SLN STA S
Sbjct: 424 EAPGAKLWGPKASLNLSTAAAS 489
Score = 113 bits (242), Expect = 7e-025
Identities = 53/81 (65%), Positives = 58/81 (71%)
Frame = -3 / +3
Query: 244 PRGEAQLRGRRHAALPDDGLQHGLAEALVLDRHAAGRRHRRVLGEGDGEDSGGRVREEDG 65
P G+A+L RRH L DDGLQHGLAE VLD HAAG RHRRVLGEGDGE +GG VREE G
Sbjct: 246 PVGQAELGRRRHPVLADDGLQHGLAEPGVLDGHAAGLRHRRVLGEGDGEHAGGGVREEYG 425
Query: 64 GARRESLWLEHLPELFYGDAL 2
GA E++ E L EL G L
Sbjct: 426 GAGGEAVGPEGLLELVDGGGL 488
>gb|CX662506.1|CX662506 EST 322 Expressed sequences from Barley Hordeum vulgare subsp.
vulgare cDNA clone P322 5', mRNA sequence
Length = 513
Score = 160 bits (345), Expect = 5e-039
Identities = 71/86 (82%), Positives = 76/86 (88%)
Frame = +2 / +2
Query: 2 EGVAVEKFREVFKPETFAPGASILFTHSTAGVLTVAFSKDSSVPAAGGVAIENKRLCEAV 181
E AV+KF+E F P +FAPGASILFTHS AGVLTVAFSKDSSVP +GGVAIEN RLCEAV
Sbjct: 92 EAAAVDKFKEAFGPHSFAPGASILFTHSPAGVLTVAFSKDSSVPESGGVAIENARLCEAV 271
Query: 182 LESIIGERGVSPAAKLSLAARVSELL 259
LESIIGE GVSPAAKLSLA RV+ELL
Sbjct: 272 LESIIGEHGVSPAAKLSLANRVAELL 349
Score = 138 bits (297), Expect = 5e-032
Identities = 63/82 (76%), Positives = 66/82 (80%)
Frame = -1 / -1
Query: 246 TLAARLSFAAGDTPRSPMMDSSTASQRRLFSIATPPAAGTDESLEKATVRTPAVECVKRM 67
TL ARLS AAGDTP SPMMDSSTASQ R FS+ATPP +GTDESLEKATV TPA ECVK M
Sbjct: 336 TLLARLSLAAGDTPCSPMMDSSTASQSRAFSMATPPDSGTDESLEKATVSTPAGECVKSM 157
Query: 66 EAPGAKVSGLNTSLNFSTATPS 1
EAPGAK+ G SLN STA S
Sbjct: 156 EAPGAKLWGPKASLNLSTAAAS 91
Score = 113 bits (242), Expect = 7e-025
Identities = 53/81 (65%), Positives = 58/81 (71%)
Frame = -3 / -3
Query: 244 PRGEAQLRGRRHAALPDDGLQHGLAEALVLDRHAAGRRHRRVLGEGDGEDSGGRVREEDG 65
P G+A+L RRH L DDGLQHGLAE VLD HAAG RHRRVLGEGDGE +GG VREE G
Sbjct: 334 PVGQAELGRRRHPVLADDGLQHGLAEPGVLDGHAAGLRHRRVLGEGDGEHAGGGVREEYG 155
Query: 64 GARRESLWLEHLPELFYGDAL 2
GA E++ E L EL G L
Sbjct: 154 GAGGEAVGPEGLLELVDGGGL 92
>gb|CX662646.1|CX662646 EST 477 Expressed sequences from Barley Hordeum vulgare subsp.
vulgare cDNA clone P477 5', mRNA sequence
Length = 513
Score = 160 bits (345), Expect = 5e-039
Identities = 71/86 (82%), Positives = 76/86 (88%)
Frame = +2 / +2
Query: 2 EGVAVEKFREVFKPETFAPGASILFTHSTAGVLTVAFSKDSSVPAAGGVAIENKRLCEAV 181
E AV+KF+E F P +FAPGASILFTHS AGVLTVAFSKDSSVP +GGVAIEN RLCEAV
Sbjct: 92 EAAAVDKFKEAFGPHSFAPGASILFTHSPAGVLTVAFSKDSSVPESGGVAIENARLCEAV 271
Query: 182 LESIIGERGVSPAAKLSLAARVSELL 259
LESIIGE GVSPAAKLSLA RV+ELL
Sbjct: 272 LESIIGEHGVSPAAKLSLANRVAELL 349
Score = 138 bits (297), Expect = 5e-032
Identities = 63/82 (76%), Positives = 66/82 (80%)
Frame = -1 / -1
Query: 246 TLAARLSFAAGDTPRSPMMDSSTASQRRLFSIATPPAAGTDESLEKATVRTPAVECVKRM 67
TL ARLS AAGDTP SPMMDSSTASQ R FS+ATPP +GTDESLEKATV TPA ECVK M
Sbjct: 336 TLLARLSLAAGDTPCSPMMDSSTASQSRAFSMATPPDSGTDESLEKATVSTPAGECVKSM 157
Query: 66 EAPGAKVSGLNTSLNFSTATPS 1
EAPGAK+ G SLN STA S
Sbjct: 156 EAPGAKLWGPKASLNLSTAAAS 91
Score = 113 bits (242), Expect = 7e-025
Identities = 53/81 (65%), Positives = 58/81 (71%)
Frame = -3 / -3
Query: 244 PRGEAQLRGRRHAALPDDGLQHGLAEALVLDRHAAGRRHRRVLGEGDGEDSGGRVREEDG 65
P G+A+L RRH L DDGLQHGLAE VLD HAAG RHRRVLGEGDGE +GG VREE G
Sbjct: 334 PVGQAELGRRRHPVLADDGLQHGLAEPGVLDGHAAGLRHRRVLGEGDGEHAGGGVREEYG 155
Query: 64 GARRESLWLEHLPELFYGDAL 2
GA E++ E L EL G L
Sbjct: 154 GAGGEAVGPEGLLELVDGGGL 92
>gb|AF474923.1| Hordeum vulgare subsp. vulgare chalcone isomerase (CHI) gene,
complete cds
Length = 11475
Score = 160 bits (345), Expect = 5e-039
Identities = 71/86 (82%), Positives = 76/86 (88%)
Frame = +2 / +3
Query: 2 EGVAVEKFREVFKPETFAPGASILFTHSTAGVLTVAFSKDSSVPAAGGVAIENKRLCEAV 181
E AV+KF+E F P +FAPGASILFTHS AGVLTVAFSKDSSVP +GGVAIEN RLCEAV
Sbjct: 7515 EAAAVDKFKEAFGPHSFAPGASILFTHSPAGVLTVAFSKDSSVPESGGVAIENARLCEAV 7694
Query: 182 LESIIGERGVSPAAKLSLAARVSELL 259
LESIIGE GVSPAAKLSLA RV+ELL
Sbjct: 7695 LESIIGEHGVSPAAKLSLANRVAELL 7772
Score = 135 bits (290), Expect = 5e-031
Identities = 62/82 (75%), Positives = 65/82 (79%)
Frame = -1 / -3
Query: 246 TLAARLSFAAGDTPRSPMMDSSTASQRRLFSIATPPAAGTDESLEKATVRTPAVECVKRM 67
TL ARLS AAGDTP SPMMDSSTASQ FS+ATPP +GTDESLEKATV TPA ECVK M
Sbjct: 7759 TLLARLSLAAGDTPCSPMMDSSTASQSLAFSMATPPDSGTDESLEKATVSTPAGECVKSM 7580
Query: 66 EAPGAKVSGLNTSLNFSTATPS 1
EAPGAK+ G SLN STA S
Sbjct: 7579 EAPGAKLWGPKASLNLSTAAAS 7514
Score = 113 bits (242), Expect = 7e-025
Identities = 53/81 (65%), Positives = 58/81 (71%)
Frame = -3 / -2
Query: 244 PRGEAQLRGRRHAALPDDGLQHGLAEALVLDRHAAGRRHRRVLGEGDGEDSGGRVREEDG 65
P G+A+L RRH L DDGLQHGLAE VLD HAAG RHRRVLGEGDGE +GG VREE G
Sbjct: 7757 PVGQAELGRRRHPVLADDGLQHGLAEPGVLDGHAAGLRHRRVLGEGDGEHAGGGVREEYG 7578
Query: 64 GARRESLWLEHLPELFYGDAL 2
GA E++ E L EL G L
Sbjct: 7577 GAGGEAVGPEGLLELVDGGGL 7515
>gb|BI957123.1|BI957123 HVSMEn0007J13f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0007J13f, mRNA sequence
Length = 592
Score = 158 bits (340), Expect = 2e-038
Identities = 71/83 (85%), Positives = 74/83 (89%)
Frame = +2 / -3
Query: 11 AVEKFREVFKPETFAPGASILFTHSTAGVLTVAFSKDSSVPAAGGVAIENKRLCEAVLES 190
AVEKF+E F P +FAPGASILFTHS AGVLTVAFSKDSSVP +GGVAIEN RLCEAVLES
Sbjct: 497 AVEKFKEAFGPHSFAPGASILFTHSPAGVLTVAFSKDSSVPESGGVAIENARLCEAVLES 318
Query: 191 IIGERGVSPAAKLSLAARVSELL 259
IIGE GVSPAAKLSLA RV ELL
Sbjct: 317 IIGEHGVSPAAKLSLANRVVELL 249
Score = 135 bits (290), Expect = 5e-031
Identities = 61/79 (77%), Positives = 65/79 (82%)
Frame = -1 / +1
Query: 246 TLAARLSFAAGDTPRSPMMDSSTASQRRLFSIATPPAAGTDESLEKATVRTPAVECVKRM 67
TL A+LS AAGDTP SPMMDSSTASQ FS+ATPP +GTDESLEKATV TPA ECVK M
Sbjct: 262 TLLAKLSLAAGDTPCSPMMDSSTASQSLAFSMATPPDSGTDESLEKATVSTPAGECVKSM 441
Query: 66 EAPGAKVSGLNTSLNFSTA 10
EAPGAK+ G SLNFSTA
Sbjct: 442 EAPGAKLWGPKASLNFSTA 498
Score = 113 bits (242), Expect = 7e-025
Identities = 52/81 (64%), Positives = 59/81 (72%)
Frame = -3 / +3
Query: 244 PRGEAQLRGRRHAALPDDGLQHGLAEALVLDRHAAGRRHRRVLGEGDGEDSGGRVREEDG 65
P G+A+L RRH L DDGLQHGLAE VLD HAAG RHRRVLGEGDGE +GG VRE+ G
Sbjct: 264 PVGQAELGRRRHPVLADDGLQHGLAEPGVLDGHAAGLRHRRVLGEGDGEHAGGGVREKYG 443
Query: 64 GARRESLWLEHLPELFYGDAL 2
GA +++ E L ELF G L
Sbjct: 444 GAGGKAVGPEGLLELFNGGGL 506
>gb|BI956899.1|BI956899 HVSMEn0005O19f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005O19f, mRNA sequence
Length = 885
Score = 121 bits (259), Expect = 3e-027
Identities = 55/65 (84%), Positives = 56/65 (86%)
Frame = +2 / +3
Query: 53 APGASILFTHSTAGVLTVAFSKDSSVPAAGGVAIENKRLCEAVLESIIGERGVSPAAKLS 232
APGASILFTHS AGVLTVAFSKDSSVP +GGVAI N RLCEAVLESIIGE GVSPAAKL
Sbjct: 177 APGASILFTHSPAGVLTVAFSKDSSVPESGGVAIXNARLCEAVLESIIGEHGVSPAAKLR 356
Query: 233 LAARV 247
L V
Sbjct: 357 LGEGV 371
Score = 110 bits (234), Expect = 2e-023
Identities = 49/65 (75%), Positives = 52/65 (80%)
Frame = -1 / -3
Query: 246 TLAARLSFAAGDTPRSPMMDSSTASQRRLFSIATPPAAGTDESLEKATVRTPAVECVKRM 67
T + LS AAGDTP SPMMDSSTASQ F +ATPP +GTDESLEKATV TPA ECVK M
Sbjct: 370 TPSPSLSLAAGDTPCSPMMDSSTASQSLAFXMATPPDSGTDESLEKATVSTPAGECVKSM 191
Query: 66 EAPGA 52
EAPGA
Sbjct: 190 EAPGA 176
>gb|CA024561.1|CA024561 HZ49K12r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ49K12
5-PRIME, mRNA sequence
Length = 499
Score = 110 bits (234), Expect = 9e-024
Identities = 46/58 (79%), Positives = 50/58 (86%)
Frame = +2 / +2
Query: 2 EGVAVEKFREVFKPETFAPGASILFTHSTAGVLTVAFSKDSSVPAAGGVAIENKRLCE 175
E AV+KF+E F P +FAPGASILFTHS AGVLTVAFSKDSSVP +GGVAIEN RLCE
Sbjct: 326 EAAAVDKFKEAFGPHSFAPGASILFTHSPAGVLTVAFSKDSSVPESGGVAIENARLCE 499
>gb|BE195609.3|BE195609 HVSMEh0089O15f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0089O15f, mRNA sequence
Length = 391
Score = 105 bits (225), Expect = 2e-022
Identities = 48/54 (88%), Positives = 50/54 (92%)
Frame = +2 / +2
Query: 98 LTVAFSKDSSVPAAGGVAIENKRLCEAVLESIIGERGVSPAAKLSLAARVSELL 259
LTVAFSKDSSVP +GGVAIEN RLCEAVLESIIGE GVSPAAKLSLA RV+ELL
Sbjct: 2 LTVAFSKDSSVPESGGVAIENARLCEAVLESIIGEHGVSPAAKLSLANRVAELL 163
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 227,371,598
Number of Sequences: 312970
Number of extensions: 3562864
Number of successful extensions: 86292
Number of sequences better than 1.0e-020: 12
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6659423313
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)