TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2622915.2.1
(584 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV064104.1|CV064104 BNEL98b1 Barley EST endosperm librar... 151 3e-036
gb|CA015797.1|CA015797 HV11O17u HV Hordeum vulgare subsp. v... 127 4e-029
gb|BU974893.1|BU974893 HB29G22r BC Hordeum vulgare subsp. v... 120 8e-027
gb|BU995027.1|BU995027 HM08P11r HM Hordeum vulgare subsp. v... 82 1e-023
gb|CB881163.1|CB881163 HM08P11w HM Hordeum vulgare subsp. v... 82 1e-023
gb|BU995276.1|BU995276 HM09L23r HM Hordeum vulgare subsp. v... 82 1e-023
gb|CB881416.1|CB881416 HM09L23w HM Hordeum vulgare subsp. v... 78 1e-021
>gb|CV064104.1|CV064104 BNEL98b1 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL98b1 5' similar to putative
sterol 4-alpha-methyl-oxidase, mRNA sequence
Length = 640
Score = 151 bits (324), Expect = 3e-036
Identities = 56/59 (94%), Positives = 58/59 (98%)
Frame = -3 / -2
Query: 582 LWMVLRVLKTVEAHTGYXFPWSPSNFLPLYGGSDFHDYHHRVLYTKSGNYASTFVYMDW 406
LWMVLRVL+TVEAH+GY FPWSPSNFLPLYGGSDFHDYHHRVLYTKSGNYASTFVYMDW
Sbjct: 576 LWMVLRVLETVEAHSGYHFPWSPSNFLPLYGGSDFHDYHHRVLYTKSGNYASTFVYMDW 400
Score = 108 bits (231), Expect(2) = 3e-024
Identities = 49/60 (81%), Positives = 51/60 (85%)
Frame = +2 / +2
Query: 404 HQSM*TNVEA*FPDFVYSTR*W*SWKSEPPYRGRKFDGLHGKX*PV*ASTVFNTLNTIHS 583
+QSM*T VEA*FPD V STR*W*SWKSEPPY GRKFDGL GK P+*ASTV NTL TIHS
Sbjct: 398 NQSM*TKVEA*FPDLV*STR*W*SWKSEPPYSGRKFDGLQGK*YPL*ASTVSNTLKTIHS 577
Score = 22.1 bits (42), Expect(2) = 3e-024
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +2 / +1
Query: 284 PQWSLCLAITIVRPEQP 334
P CLAIT++ +QP
Sbjct: 352 PFHGFCLAITLICAKQP 402
>gb|CA015797.1|CA015797 HV11O17u HV Hordeum vulgare subsp. vulgare cDNA clone HV11O17
3-PRIME, mRNA sequence
Length = 536
Score = 127 bits (273), Expect = 4e-029
Identities = 46/48 (95%), Positives = 47/48 (97%)
Frame = -3 / -2
Query: 549 EAHTGYXFPWSPSNFLPLYGGSDFHDYHHRVLYTKSGNYASTFVYMDW 406
EAH+GY FPWSPSNFLPLYGGSDFHDYHHRVLYTKSGNYASTFVYMDW
Sbjct: 535 EAHSGYHFPWSPSNFLPLYGGSDFHDYHHRVLYTKSGNYASTFVYMDW 392
>gb|BU974893.1|BU974893 HB29G22r BC Hordeum vulgare subsp. vulgare cDNA clone HB29G22
5-PRIME, mRNA sequence
Length = 499
Score = 120 bits (256), Expect = 8e-027
Identities = 43/45 (95%), Positives = 44/45 (97%)
Frame = -3 / +2
Query: 540 TGYXFPWSPSNFLPLYGGSDFHDYHHRVLYTKSGNYASTFVYMDW 406
+GY FPWSPSNFLPLYGGSDFHDYHHRVLYTKSGNYASTFVYMDW
Sbjct: 2 SGYHFPWSPSNFLPLYGGSDFHDYHHRVLYTKSGNYASTFVYMDW 136
>gb|BU995027.1|BU995027 HM08P11r HM Hordeum vulgare subsp. vulgare cDNA clone HM08P11
5-PRIME, mRNA sequence
Length = 621
Score = 81.7 bits (172), Expect(2) = 1e-023
Identities = 31/40 (77%), Positives = 34/40 (85%)
Frame = -3 / +1
Query: 582 LWMVLRVLKTVEAHTGYXFPWSPSNFLPLYGGSDFHDYHH 463
LWMVLRVL+TVEAH+GY FPWSPSNFLPLYGGS +H
Sbjct: 412 LWMVLRVLETVEAHSGYHFPWSPSNFLPLYGGSSDFHDYH 531
Score = 78.0 bits (164), Expect(2) = 3e-021
Identities = 28/29 (96%), Positives = 28/29 (96%)
Frame = -3 / +1
Query: 492 GGSDFHDYHHRVLYTKSGNYASTFVYMDW 406
G SDFHDYHHRVLYTKSGNYASTFVYMDW
Sbjct: 505 GSSDFHDYHHRVLYTKSGNYASTFVYMDW 591
Score = 47.3 bits (97), Expect(2) = 1e-023
Identities = 20/28 (71%), Positives = 20/28 (71%)
Frame = -1 / +2
Query: 488 ARTSMTTITECCTQSQGTMPRHLFTWTG 405
A TSMTTI C T SQGT P LFTWTG
Sbjct: 509 ALTSMTTIIVCSTPSQGTTPLLLFTWTG 592
Score = 42.8 bits (87), Expect(2) = 3e-021
Identities = 18/30 (60%), Positives = 22/30 (73%)
Frame = -2 / +3
Query: 583 AVDGVKGIEDC*SSHRLSLPMEPIKFPAPV 494
AVDG +G+ D SS +SLP+EPIK PA V
Sbjct: 411 AVDGFEGVRDGGSSQWISLPLEPIKLPATV 500
>gb|CB881163.1|CB881163 HM08P11w HM Hordeum vulgare subsp. vulgare cDNA clone HM08P11
3-PRIME, mRNA sequence
Length = 604
Score = 81.7 bits (172), Expect(2) = 1e-023
Identities = 31/40 (77%), Positives = 34/40 (85%)
Frame = -3 / -1
Query: 582 LWMVLRVLKTVEAHTGYXFPWSPSNFLPLYGGSDFHDYHH 463
LWMVLRVL+TVEAH+GY FPWSPSNFLPLYGGS +H
Sbjct: 547 LWMVLRVLETVEAHSGYHFPWSPSNFLPLYGGSSDFHDYH 428
Score = 78.0 bits (164), Expect(2) = 3e-021
Identities = 28/29 (96%), Positives = 28/29 (96%)
Frame = -3 / -1
Query: 492 GGSDFHDYHHRVLYTKSGNYASTFVYMDW 406
G SDFHDYHHRVLYTKSGNYASTFVYMDW
Sbjct: 454 GSSDFHDYHHRVLYTKSGNYASTFVYMDW 368
Score = 47.3 bits (97), Expect(2) = 1e-023
Identities = 20/28 (71%), Positives = 20/28 (71%)
Frame = -1 / -2
Query: 488 ARTSMTTITECCTQSQGTMPRHLFTWTG 405
A TSMTTI C T SQGT P LFTWTG
Sbjct: 450 ALTSMTTIIVCSTPSQGTTPLLLFTWTG 367
Score = 42.8 bits (87), Expect(2) = 3e-021
Identities = 18/30 (60%), Positives = 22/30 (73%)
Frame = -2 / -3
Query: 583 AVDGVKGIEDC*SSHRLSLPMEPIKFPAPV 494
AVDG +G+ D SS +SLP+EPIK PA V
Sbjct: 548 AVDGFEGVRDGGSSQWISLPLEPIKLPATV 459
>gb|BU995276.1|BU995276 HM09L23r HM Hordeum vulgare subsp. vulgare cDNA clone HM09L23
5-PRIME, mRNA sequence
Length = 591
Score = 81.7 bits (172), Expect(2) = 1e-023
Identities = 31/40 (77%), Positives = 34/40 (85%)
Frame = -3 / +1
Query: 582 LWMVLRVLKTVEAHTGYXFPWSPSNFLPLYGGSDFHDYHH 463
LWMVLRVL+TVEAH+GY FPWSPSNFLPLYGGS +H
Sbjct: 385 LWMVLRVLETVEAHSGYHFPWSPSNFLPLYGGSSDFHDYH 504
Score = 78.0 bits (164), Expect(2) = 3e-021
Identities = 28/29 (96%), Positives = 28/29 (96%)
Frame = -3 / +1
Query: 492 GGSDFHDYHHRVLYTKSGNYASTFVYMDW 406
G SDFHDYHHRVLYTKSGNYASTFVYMDW
Sbjct: 478 GSSDFHDYHHRVLYTKSGNYASTFVYMDW 564
Score = 47.3 bits (97), Expect(2) = 1e-023
Identities = 20/28 (71%), Positives = 20/28 (71%)
Frame = -1 / +2
Query: 488 ARTSMTTITECCTQSQGTMPRHLFTWTG 405
A TSMTTI C T SQGT P LFTWTG
Sbjct: 482 ALTSMTTIIVCSTPSQGTTPLLLFTWTG 565
Score = 42.8 bits (87), Expect(2) = 3e-021
Identities = 18/30 (60%), Positives = 22/30 (73%)
Frame = -2 / +3
Query: 583 AVDGVKGIEDC*SSHRLSLPMEPIKFPAPV 494
AVDG +G+ D SS +SLP+EPIK PA V
Sbjct: 384 AVDGFEGVRDGGSSQWISLPLEPIKLPATV 473
>gb|CB881416.1|CB881416 HM09L23w HM Hordeum vulgare subsp. vulgare cDNA clone HM09L23
3-PRIME, mRNA sequence
Length = 576
Score = 78.0 bits (164), Expect(2) = 1e-021
Identities = 28/29 (96%), Positives = 28/29 (96%)
Frame = -3 / -1
Query: 492 GGSDFHDYHHRVLYTKSGNYASTFVYMDW 406
G SDFHDYHHRVLYTKSGNYASTFVYMDW
Sbjct: 453 GSSDFHDYHHRVLYTKSGNYASTFVYMDW 367
Score = 74.8 bits (157), Expect(2) = 1e-021
Identities = 29/40 (72%), Positives = 32/40 (80%)
Frame = -3 / -1
Query: 582 LWMVLRVLKTVEAHTGYXFPWSPSNFLPLYGGSDFHDYHH 463
LWMV VL+TVEAH+GY FPWSPSNFLPLYGGS +H
Sbjct: 546 LWMV*GVLETVEAHSGYHFPWSPSNFLPLYGGSSDFHDYH 427
Score = 47.3 bits (97), Expect(2) = 1e-021
Identities = 20/28 (71%), Positives = 20/28 (71%)
Frame = -1 / -2
Query: 488 ARTSMTTITECCTQSQGTMPRHLFTWTG 405
A TSMTTI C T SQGT P LFTWTG
Sbjct: 449 ALTSMTTIIVCSTPSQGTTPLLLFTWTG 366
Score = 44.1 bits (90), Expect(2) = 1e-021
Identities = 18/30 (60%), Positives = 23/30 (76%)
Frame = -2 / -3
Query: 583 AVDGVKGIEDC*SSHRLSLPMEPIKFPAPV 494
AVDG++G+ D SS +SLP+EPIK PA V
Sbjct: 547 AVDGLRGVRDGGSSQWISLPLEPIKLPATV 458
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 269,732,872
Number of Sequences: 312970
Number of extensions: 4216361
Number of successful extensions: 52365
Number of sequences better than 1.0e-020: 7
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6065589387
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)