TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2621786.2.1
(623 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AJ435714.1|AJ435714 AJ435714 S00002 Hordeum vulgare subs... 242 7e-065
gb|CA000350.1|CA000350 HS08P01u HS Hordeum vulgare subsp. v... 218 9e-058
gb|CK124142.1|CK124142 BES1824107d03 BES1824 Hordeum vulgar... 180 5e-045
gb|AL510982.1|AL510982 AL510982 Hordeum vulgare Barke devel... 152 6e-038
>gb|AJ435714.1|AJ435714 AJ435714 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200059A10F1, mRNA sequence
Length = 568
Score = 242 bits (523), Expect(2) = 7e-065
Identities = 98/119 (82%), Positives = 111/119 (93%)
Frame = +2 / +2
Query: 38 VEAMWLMLQQEQPGDYVVATEESHTVEEFLQAAFGYAGLSWKDHVVIDKKYFRPAEVDSL 217
VEAMWLMLQQ++P DYVVATEE HTVEEFLQAAFGYAGL+WKDHVVIDKKYFRP+EVD L
Sbjct: 5 VEAMWLMLQQDKPDDYVVATEECHTVEEFLQAAFGYAGLNWKDHVVIDKKYFRPSEVDCL 184
Query: 218 KGDATKARKVLKWKPKVGFQELVEMMVDHDIELAKKEKVLVDAGFRDPKQQP*KHHSCF 394
+GD++K+R+VL WKPKVGFQ+LVEMMVD+DIELAKKEKVLVDAG+RDPKQQP* + F
Sbjct: 185 QGDSSKSRRVLGWKPKVGFQQLVEMMVDNDIELAKKEKVLVDAGYRDPKQQP*SRYR*F 361
Score = 187 bits (402), Expect(2) = 9e-048
Identities = 83/113 (73%), Positives = 85/113 (75%)
Frame = -3 / -2
Query: 375 YGCCLGSRNPASTRTFSFLAXXXXXXXXXXXXSWNPTLGFHLSTFLALVASPLRLSTSAG 196
YGCCLGSR PASTRTFSFLA W PTLGFH ST L L SP R STS G
Sbjct: 342 YGCCLGSRYPASTRTFSFLASSMSLSTIISTSCWKPTLGFHPSTLLDLDESP*RQSTSEG 163
Query: 195 RKYFLSMTT*SFQLRPA*PKAACRNSSTVWDSSVATT*SPGCSCCSMSHIAST 37
RKYFLSMTT*SFQLRPA PKAAC+NSSTVW SSVATT*S G SC SMSH+AST
Sbjct: 162 RKYFLSMTT*SFQLRPAYPKAACKNSSTVWHSSVATT*SSGLSCWSMSHMAST 4
Score = 121 bits (259), Expect = 3e-027
Identities = 57/85 (67%), Positives = 60/85 (70%)
Frame = +1 / +1
Query: 127 AGCLRLRRPQLEGSRGH*QEVLPACRSRQPQGGRHQGEEGAQVEAQGWVPGARGDDGRSR 306
AGCL +RRPQLEGS GH QEVL R P G Q EE A VEAQGW+P A DDG R
Sbjct: 94 AGCLWVRRPQLEGSCGHRQEVLSPFRGGLPSGRFIQVEESAWVEAQGWLPAAG*DDG**R 273
Query: 307 H*ARQEGEGSGGCWVP*SQAAAIET 381
H*ARQEGEG GCWVP QAAAIE+
Sbjct: 274 H*ARQEGEGPCGCWVPRPQAAAIES 348
Score = 75.3 bits (158), Expect(2) = 3e-028
Identities = 35/67 (52%), Positives = 44/67 (65%)
Frame = -2 / -1
Query: 238 RLGGVPLEAVDFCRPEVLLVNDHVILPAEAGVAEGSLQEXXXXXXXXXXXXVIPRLFLLQ 59
RLG + L+AV R +VLLV+DH+ILP EAGV +GSLQE ++ RL LL+
Sbjct: 205 RLG*ISLKAVHLGRAKVLLVDDHMILPVEAGVPKGSLQELLHGVALLGGDNIVIRLVLLE 26
Query: 58 HEPHCLD 38
HEPH LD
Sbjct: 25 HEPHGLD 5
Score = 69.3 bits (145), Expect(2) = 3e-028
Identities = 27/37 (72%), Positives = 29/37 (78%)
Frame = -1 / -3
Query: 377 SMAAAWDHGTQHPPEPSPSWRAQCRDRPSSPRAPGTQ 267
SMAAAW GTQHP PSPSWRAQCR +PSS A G+Q
Sbjct: 344 SMAAAWGRGTQHPQGPSPSWRAQCRYQPSSQPAAGSQ 234
Score = 24.9 bits (48), Expect(2) = 7e-065
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +1 / +1
Query: 493 RNKDCCSMILQCVHLLF*LCYVL*SNE 573
RNK+ CS+ILQ L + + Y NE
Sbjct: 463 RNKEVCSLILQYR*LSYYVTYFYHMNE 543
Score = 23.1 bits (44), Expect(2) = 9e-048
Identities = 11/29 (37%), Positives = 12/29 (41%)
Frame = -2 / -1
Query: 574 IHSIKAHNIVRKVNEHTVRSYCNNPYFLP 488
IHS + TV S C PYF P
Sbjct: 544 IHSYDKSTSHSNLINGTVGSNCKPPYFYP 458
>gb|CA000350.1|CA000350 HS08P01u HS Hordeum vulgare subsp. vulgare cDNA clone HS08P01
3-PRIME, mRNA sequence
Length = 524
Score = 218 bits (471), Expect(2) = 9e-058
Identities = 88/109 (80%), Positives = 101/109 (92%)
Frame = +2 / -3
Query: 68 EQPGDYVVATEESHTVEEFLQAAFGYAGLSWKDHVVIDKKYFRPAEVDSLKGDATKARKV 247
++P DYVVATEE HTVEEFLQAAFGYAGL+WKDHVVIDKKYFRP+EVD L+GD++K+R+V
Sbjct: 522 DKPDDYVVATEECHTVEEFLQAAFGYAGLNWKDHVVIDKKYFRPSEVDCLQGDSSKSRRV 343
Query: 248 LKWKPKVGFQELVEMMVDHDIELAKKEKVLVDAGFRDPKQQP*KHHSCF 394
L WKPKVGFQ+LVEMMVD+DIELAKKEKVLVDAG+RDPKQQP* + F
Sbjct: 342 LGWKPKVGFQQLVEMMVDNDIELAKKEKVLVDAGYRDPKQQP*SRYR*F 196
Score = 167 bits (360), Expect(2) = 5e-042
Identities = 75/103 (72%), Positives = 76/103 (73%)
Frame = -3 / +2
Query: 375 YGCCLGSRNPASTRTFSFLAXXXXXXXXXXXXSWNPTLGFHLSTFLALVASPLRLSTSAG 196
YGCCLGSR PASTRTFSFLA W PTLGFH ST L L SP R STS G
Sbjct: 215 YGCCLGSRYPASTRTFSFLASSMSLSTIISTSCWKPTLGFHPSTLLDLDESP*RQSTSEG 394
Query: 195 RKYFLSMTT*SFQLRPA*PKAACRNSSTVWDSSVATT*SPGCS 67
RKYFLSMTT*SFQLRPA PKAAC+NSSTVW SSVATT*S G S
Sbjct: 395 RKYFLSMTT*SFQLRPAYPKAACKNSSTVWHSSVATT*SSGLS 523
Score = 121 bits (259), Expect = 3e-027
Identities = 57/85 (67%), Positives = 60/85 (70%)
Frame = +1 / -2
Query: 127 AGCLRLRRPQLEGSRGH*QEVLPACRSRQPQGGRHQGEEGAQVEAQGWVPGARGDDGRSR 306
AGCL +RRPQLEGS GH QEVL R P G Q EE A VEAQGW+P A DDG R
Sbjct: 463 AGCLWVRRPQLEGSCGHRQEVLSPFRGGLPSGRFIQVEESAWVEAQGWLPAAG*DDG**R 284
Query: 307 H*ARQEGEGSGGCWVP*SQAAAIET 381
H*ARQEGEG GCWVP QAAAIE+
Sbjct: 283 H*ARQEGEGPCGCWVPRPQAAAIES 209
Score = 69.3 bits (145), Expect(3) = 7e-026
Identities = 27/37 (72%), Positives = 29/37 (78%)
Frame = -1 / +3
Query: 377 SMAAAWDHGTQHPPEPSPSWRAQCRDRPSSPRAPGTQ 267
SMAAAW GTQHP PSPSWRAQCR +PSS A G+Q
Sbjct: 213 SMAAAWGRGTQHPQGPSPSWRAQCRYQPSSQPAAGSQ 323
Score = 56.5 bits (117), Expect(3) = 7e-026
Identities = 25/39 (64%), Positives = 31/39 (79%)
Frame = -2 / +1
Query: 238 RLGGVPLEAVDFCRPEVLLVNDHVILPAEAGVAEGSLQE 122
RLG + L+AV R +VLLV+DH+ILP EAGV +GSLQE
Sbjct: 352 RLG*ISLKAVHLGRAKVLLVDDHMILPVEAGVPKGSLQE 468
Score = 28.6 bits (56), Expect(3) = 7e-026
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -1 / +3
Query: 128 AGTPRRCGTPQW 93
A TP RCGTP+W
Sbjct: 462 ARTPPRCGTPRW 497
Score = 24.9 bits (48), Expect(2) = 9e-058
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +1 / -2
Query: 493 RNKDCCSMILQCVHLLF*LCYVL*SNE 573
RNK+ CS+ILQ L + + Y NE
Sbjct: 94 RNKEVCSLILQYR*LSYYVTYFYHMNE 14
Score = 23.1 bits (44), Expect(2) = 5e-042
Identities = 11/29 (37%), Positives = 12/29 (41%)
Frame = -2 / +1
Query: 574 IHSIKAHNIVRKVNEHTVRSYCNNPYFLP 488
IHS + TV S C PYF P
Sbjct: 13 IHSYDKSTSHSNLINGTVGSNCKPPYFYP 99
>gb|CK124142.1|CK124142 BES1824107d03 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010D037 5-PRIME, mRNA sequence
Length = 847
Score = 180 bits (388), Expect = 5e-045
Identities = 70/83 (84%), Positives = 80/83 (96%)
Frame = +2 / +3
Query: 2 SSARDWGFAGDYVEAMWLMLQQEQPGDYVVATEESHTVEEFLQAAFGYAGLSWKDHVVID 181
S+ARDWGFAGDYVEAMWLMLQQ++P DYVVATEE HTVEEFLQAAFGYAGL+WKDHVVID
Sbjct: 558 SAARDWGFAGDYVEAMWLMLQQDKPDDYVVATEECHTVEEFLQAAFGYAGLNWKDHVVID 737
Query: 182 KKYFRPAEVDSLKGDATKARKVL 250
KKYFRP+EVD L+GD++K+R+VL
Sbjct: 738 KKYFRPSEVDCLQGDSSKSRRVL 806
Score = 139 bits (299), Expect = 1e-032
Identities = 66/83 (79%), Positives = 68/83 (81%)
Frame = -3 / -1
Query: 249 STFLALVASPLRLSTSAGRKYFLSMTT*SFQLRPA*PKAACRNSSTVWDSSVATT*SPGC 70
ST L L SP R STS GRKYFLSMTT*SFQLRPA PKAAC+NSSTVW SSVATT*S G
Sbjct: 805 STLLDLDESP*RQSTSEGRKYFLSMTT*SFQLRPAYPKAACKNSSTVWHSSVATT*SSGL 626
Query: 69 SCCSMSHIAST*SPANPQSRADE 1
SC SMSH+AST*SPA PQS A E
Sbjct: 625 SCWSMSHMAST*SPAKPQSLAAE 557
>gb|AL510982.1|AL510982 AL510982 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY06K01u 3',
mRNA sequence
Length = 455
Score = 152 bits (326), Expect(2) = 6e-038
Identities = 61/79 (77%), Positives = 72/79 (91%)
Frame = +2 / -1
Query: 158 WKDHVVIDKKYFRPAEVDSLKGDATKARKVLKWKPKVGFQELVEMMVDHDIELAKKEKVL 337
WK+HVVIDKK FRP+EVD L+GD++K+R+VL WKPKVGFQ+LVEMMVD+DIELAKKEKVL
Sbjct: 455 WKNHVVIDKKXFRPSEVDCLQGDSSKSRRVLGWKPKVGFQQLVEMMVDNDIELAKKEKVL 276
Query: 338 VDAGFRDPKQQP*KHHSCF 394
VDAG+RDPKQQP* + F
Sbjct: 275 VDAGYRDPKQQP*SRYR*F 219
Score = 104 bits (222), Expect(2) = 3e-023
Identities = 47/72 (65%), Positives = 47/72 (65%)
Frame = -3 / +1
Query: 375 YGCCLGSRNPASTRTFSFLAXXXXXXXXXXXXSWNPTLGFHLSTFLALVASPLRLSTSAG 196
YGCCLGSR PASTRTFSFLA W PTLGFH ST L L SP R STS G
Sbjct: 238 YGCCLGSRYPASTRTFSFLASSMSLSTIISTSCWKPTLGFHPSTLLDLDESP*RQSTSEG 417
Query: 195 RKYFLSMTT*SF 160
RK FLSMTT* F
Sbjct: 418 RKXFLSMTT*FF 453
Score = 24.9 bits (48), Expect(2) = 6e-038
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +1 / -3
Query: 493 RNKDCCSMILQCVHLLF*LCYVL*SNE 573
RNK+ CS+ILQ L + + Y NE
Sbjct: 117 RNKEVCSLILQYR*LSYYVTYFYHMNE 37
Score = 23.1 bits (44), Expect(2) = 3e-023
Identities = 11/29 (37%), Positives = 12/29 (41%)
Frame = -2 / +3
Query: 574 IHSIKAHNIVRKVNEHTVRSYCNNPYFLP 488
IHS + TV S C PYF P
Sbjct: 36 IHSYDKSTSHSNLINGTVGSNCKPPYFYP 122
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 277,782,500
Number of Sequences: 312970
Number of extensions: 4535880
Number of successful extensions: 92063
Number of sequences better than 1.0e-020: 6
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6617006604
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)