TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2594211.2.1
(668 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA020777.1|CA020777 HZ37K23r HZ Hordeum vulgare subsp. v... 110 1e-028
gb|CB875905.1|CB875905 HX09L09w HX Hordeum vulgare subsp. v... 110 2e-028
gb|AV921430.1|AV921430 AV921430 K. Sato unpublished cDNA li... 110 2e-028
gb|BG343293.2|BG343293 HVSMEg0005F10f Hordeum vulgare pre-a... 110 5e-028
gb|AV913816.1|AV913816 AV913816 K. Sato unpublished cDNA li... 110 5e-028
gb|BJ464959.1|BJ464959 BJ464959 K. Sato unpublished cDNA li... 110 5e-028
gb|CA031352.1|CA031352 HX09L09r HX Hordeum vulgare subsp. v... 110 7e-024
gb|AV917412.1|AV917412 AV917412 K. Sato unpublished cDNA li... 109 2e-023
gb|AV922591.1|AV922591 AV922591 K. Sato unpublished cDNA li... 109 2e-023
gb|BQ463996.1|BQ463996 HF01B01T HF Hordeum vulgare subsp. v... 109 2e-023
gb|CA025455.1|CA025455 HZ52D12r HZ Hordeum vulgare subsp. v... 109 2e-023
gb|AV917956.1|AV917956 AV917956 K. Sato unpublished cDNA li... 108 3e-023
gb|AL500971.1|AL500971 AL500971 Hordeum vulgare Barke roots... 100 6e-021
gb|BJ454545.1|BJ454545 BJ454545 K. Sato unpublished cDNA li... 100 8e-021
gb|BJ462348.1|BJ462348 BJ462348 K. Sato unpublished cDNA li... 100 8e-021
gb|BQ465629.1|BQ465629 HU04D11r HU Hordeum vulgare subsp. v... 100 8e-021
gb|BQ762357.1|BQ762357 EBro01_SQ005_H18_R root, 3 week, hyd... 100 8e-021
gb|CA005047.1|CA005047 HU12E04u HU Hordeum vulgare subsp. v... 100 8e-021
>gb|CA020777.1|CA020777 HZ37K23r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ37K23
5-PRIME, mRNA sequence
Length = 582
Score = 110 bits (235), Expect(3) = 1e-028
Identities = 42/55 (76%), Positives = 48/55 (87%)
Frame = -3 / +1
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TPS 484
+WGQNWQSN LLDGQ LSFRVT +G+SVVS+NA PRGW FGQTFSGAQF+* P+
Sbjct: 76 NWGQNWQSNALLDGQGLSFRVTAGNGQSVVSNNAVPRGWSFGQTFSGAQFH*APA 240
Score = 33.6 bits (67), Expect(3) = 1e-028
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -2 / +3
Query: 394 LGACREAPWFYEW 356
+GAC EAPWFY W
Sbjct: 312 IGACWEAPWFYGW 350
Score = 21.7 bits (41), Expect(3) = 1e-028
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -1 / +1
Query: 194 AAFYHPPI 171
AA YHPPI
Sbjct: 520 AALYHPPI 543
>gb|CB875905.1|CB875905 HX09L09w HX Hordeum vulgare subsp. vulgare cDNA clone HX09L09
3-PRIME, mRNA sequence
Length = 650
Score = 110 bits (235), Expect(3) = 2e-028
Identities = 42/55 (76%), Positives = 48/55 (87%)
Frame = -3 / -3
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TPS 484
+WGQNWQSN LLDGQ LSFRVT +G+SVVS+NA PRGW FGQTFSGAQF+* P+
Sbjct: 606 NWGQNWQSNALLDGQGLSFRVTAGNGQSVVSNNAVPRGWSFGQTFSGAQFH*APA 442
Score = 33.6 bits (67), Expect(3) = 2e-028
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -2 / -2
Query: 394 LGACREAPWFYEW 356
+GAC EAPWFY W
Sbjct: 370 IGACWEAPWFYGW 332
Score = 21.7 bits (41), Expect(3) = 2e-028
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -1 / -3
Query: 194 AAFYHPPI 171
AA YHPPI
Sbjct: 162 AALYHPPI 139
>gb|AV921430.1|AV921430 AV921430 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags23g08 3', mRNA sequence
Length = 710
Score = 110 bits (235), Expect(3) = 2e-028
Identities = 42/55 (76%), Positives = 48/55 (87%)
Frame = -3 / -1
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TPS 484
+WGQNWQSN LLDGQ LSFRVT +G+SVVS+NA PRGW FGQTFSGAQF+* P+
Sbjct: 578 NWGQNWQSNALLDGQGLSFRVTAGNGQSVVSNNAVPRGWSFGQTFSGAQFH*APA 414
Score = 95.5 bits (202), Expect(3) = 3e-021
Identities = 38/53 (71%), Positives = 39/53 (73%)
Frame = +2 / +1
Query: 488 GVQLNWAPLKVWPKPQPRGAALLETTERPSLVVTRKERACPSRSVLLCQFWPQ 646
G Q NWAPLKVWP QPRG ALLETT+ P VTR ER CPS S LLCQF PQ
Sbjct: 418 GAQWNWAPLKVWPNDQPRGTALLETTDWPLPAVTRNERPCPSSSALLCQFCPQ 576
Score = 33.6 bits (67), Expect(3) = 2e-028
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -2 / -3
Query: 394 LGACREAPWFYEW 356
+GAC EAPWFY W
Sbjct: 342 IGACWEAPWFYGW 304
Score = 23.5 bits (45), Expect(3) = 3e-021
Identities = 8/14 (57%), Positives = 9/14 (64%)
Frame = +2 / +1
Query: 350 DAPFIKPWRLSASS 391
+ P IKPWR A S
Sbjct: 298 EGPTIKPWRFPARS 339
Score = 22.6 bits (43), Expect(3) = 3e-021
Identities = 8/18 (44%), Positives = 13/18 (72%)
Frame = +1 / +3
Query: 85 RVHYNQHTWVTRKTSHPK 138
R+H++QHT T+ + PK
Sbjct: 15 RLHHSQHTPHTQTRNTPK 68
Score = 21.7 bits (41), Expect(3) = 2e-028
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -1 / -1
Query: 194 AAFYHPPI 171
AA YHPPI
Sbjct: 134 AALYHPPI 111
>gb|BG343293.2|BG343293 HVSMEg0005F10f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0005F10f, mRNA sequence
Length = 947
Score = 110 bits (235), Expect(2) = 5e-028
Identities = 42/55 (76%), Positives = 48/55 (87%)
Frame = -3 / +3
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TPS 484
+WGQNWQSN LLDGQ LSFRVT +G+SVVS+NA PRGW FGQTFSGAQF+* P+
Sbjct: 99 NWGQNWQSNALLDGQGLSFRVTAGNGQSVVSNNAVPRGWSFGQTFSGAQFH*APA 263
Score = 33.6 bits (67), Expect(2) = 5e-028
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -2 / +2
Query: 394 LGACREAPWFYEW 356
+GAC EAPWFY W
Sbjct: 335 IGACWEAPWFYGW 373
>gb|AV913816.1|AV913816 AV913816 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags23g08 5', mRNA sequence
Length = 657
Score = 110 bits (235), Expect(2) = 5e-028
Identities = 42/55 (76%), Positives = 48/55 (87%)
Frame = -3 / +2
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TPS 484
+WGQNWQSN LLDGQ LSFRVT +G+SVVS+NA PRGW FGQTFSGAQF+* P+
Sbjct: 209 NWGQNWQSNALLDGQGLSFRVTAGNGQSVVSNNAVPRGWSFGQTFSGAQFH*APA 373
Score = 33.6 bits (67), Expect(2) = 5e-028
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -2 / +1
Query: 394 LGACREAPWFYEW 356
+GAC EAPWFY W
Sbjct: 445 IGACWEAPWFYGW 483
>gb|BJ464959.1|BJ464959 BJ464959 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags36l22 5', mRNA sequence
Length = 639
Score = 110 bits (235), Expect(2) = 5e-028
Identities = 42/55 (76%), Positives = 48/55 (87%)
Frame = -3 / +2
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TPS 484
+WGQNWQSN LLDGQ LSFRVT +G+SVVS+NA PRGW FGQTFSGAQF+* P+
Sbjct: 209 NWGQNWQSNALLDGQGLSFRVTAGNGQSVVSNNAVPRGWSFGQTFSGAQFH*APA 373
Score = 33.6 bits (67), Expect(2) = 5e-028
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -2 / +1
Query: 394 LGACREAPWFYEW 356
+GAC EAPWFY W
Sbjct: 445 IGACWEAPWFYGW 483
>gb|CA031352.1|CA031352 HX09L09r HX Hordeum vulgare subsp. vulgare cDNA clone HX09L09
5-PRIME, mRNA sequence
Length = 631
Score = 110 bits (235), Expect = 7e-024
Identities = 42/55 (76%), Positives = 48/55 (87%)
Frame = -3 / +3
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TPS 484
+WGQNWQSN LLDGQ LSFRVT +G+SVVS+NA PRGW FGQTFSGAQF+* P+
Sbjct: 441 NWGQNWQSNALLDGQGLSFRVTAGNGQSVVSNNAVPRGWSFGQTFSGAQFH*APA 605
>gb|AV917412.1|AV917412 AV917412 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags18m24 5', mRNA sequence
Length = 626
Score = 109 bits (232), Expect = 2e-023
Identities = 44/62 (70%), Positives = 50/62 (80%)
Frame = -3 / +2
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TPSIVPAA 469
+WG NWQSNT LDGQ LSFRVT+SDGR+V S+ AAP GW FGQTF GAQF TPS P++
Sbjct: 221 NWGMNWQSNTFLDGQCLSFRVTSSDGRTVTSNAAAPAGWQFGQTFEGAQF*STPSPSPSS 400
Query: 468 SS 463
SS
Sbjct: 401 SS 406
>gb|AV922591.1|AV922591 AV922591 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags18m24 3', mRNA sequence
Length = 701
Score = 109 bits (232), Expect = 2e-023
Identities = 44/62 (70%), Positives = 50/62 (80%)
Frame = -3 / -3
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TPSIVPAA 469
+WG NWQSNT LDGQ LSFRVT+SDGR+V S+ AAP GW FGQTF GAQF TPS P++
Sbjct: 561 NWGMNWQSNTFLDGQCLSFRVTSSDGRTVTSNAAAPAGWQFGQTFEGAQF*STPSPSPSS 382
Query: 468 SS 463
SS
Sbjct: 381 SS 376
>gb|BQ463996.1|BQ463996 HF01B01T HF Hordeum vulgare subsp. vulgare cDNA clone HF01B01
5-PRIME, mRNA sequence
Length = 685
Score = 109 bits (232), Expect = 2e-023
Identities = 44/62 (70%), Positives = 50/62 (80%)
Frame = -3 / +2
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TPSIVPAA 469
+WG NWQSNT LDGQ LSFRVT+SDGR+V S+ AAP GW FGQTF GAQF TPS P++
Sbjct: 80 NWGMNWQSNTFLDGQCLSFRVTSSDGRTVTSNAAAPAGWQFGQTFEGAQF*STPSPSPSS 259
Query: 468 SS 463
SS
Sbjct: 260 SS 265
>gb|CA025455.1|CA025455 HZ52D12r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ52D12
5-PRIME, mRNA sequence
Length = 475
Score = 109 bits (232), Expect = 2e-023
Identities = 44/62 (70%), Positives = 50/62 (80%)
Frame = -3 / +3
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TPSIVPAA 469
+WG NWQSNT LDGQ LSFRVT+SDGR+V S+ AAP GW FGQTF GAQF TPS P++
Sbjct: 126 NWGMNWQSNTFLDGQCLSFRVTSSDGRTVTSNAAAPAGWQFGQTFEGAQF*STPSPSPSS 305
Query: 468 SS 463
SS
Sbjct: 306 SS 311
>gb|AV917956.1|AV917956 AV917956 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags17d09 3', mRNA sequence
Length = 567
Score = 108 bits (231), Expect = 3e-023
Identities = 44/61 (72%), Positives = 49/61 (80%)
Frame = -3 / -2
Query: 645 WGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQFN*TPSIVPAAS 466
WG NWQSNT LDGQ LSFRVT+SDGR+V S+ AAP GW FGQTF GAQF TPS P++S
Sbjct: 566 WGMNWQSNTFLDGQCLSFRVTSSDGRTVTSNAAAPAGWQFGQTFEGAQF*STPSPSPSSS 387
Query: 465 S 463
S
Sbjct: 386 S 384
>gb|AL500971.1|AL500971 AL500971 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW02J12u 3', mRNA sequence
Length = 567
Score = 100 bits (214), Expect = 6e-021
Identities = 39/51 (76%), Positives = 42/51 (82%)
Frame = -3 / -2
Query: 651 AHWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQF 499
A WGQNWQSN LDGQ+LSFRVT SDGR+V S+NAAP GW FGQTF G QF
Sbjct: 503 ATWGQNWQSNADLDGQSLSFRVTLSDGRTVTSNNAAPAGWSFGQTFEGGQF 351
>gb|BJ454545.1|BJ454545 BJ454545 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak24n09 5', mRNA sequence
Length = 654
Score = 100 bits (213), Expect = 8e-021
Identities = 38/50 (76%), Positives = 42/50 (84%)
Frame = -3 / +1
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQF 499
+WGQNWQSN LDGQ+LSFRVT SDGR+V S+NAAP GW FGQTF G QF
Sbjct: 364 NWGQNWQSNADLDGQSLSFRVTLSDGRTVTSNNAAPAGWSFGQTFEGGQF 513
>gb|BJ462348.1|BJ462348 BJ462348 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak24n09 3', mRNA sequence
Length = 720
Score = 100 bits (213), Expect = 8e-021
Identities = 38/50 (76%), Positives = 42/50 (84%)
Frame = -3 / -3
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQF 499
+WGQNWQSN LDGQ+LSFRVT SDGR+V S+NAAP GW FGQTF G QF
Sbjct: 403 NWGQNWQSNADLDGQSLSFRVTLSDGRTVTSNNAAPAGWSFGQTFEGGQF 254
>gb|BQ465629.1|BQ465629 HU04D11r HU Hordeum vulgare subsp. vulgare cDNA clone HU04D11
5-PRIME, mRNA sequence
Length = 625
Score = 100 bits (213), Expect = 8e-021
Identities = 38/50 (76%), Positives = 42/50 (84%)
Frame = -3 / +2
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQF 499
+WGQNWQSN LDGQ+LSFRVT SDGR+V S+NAAP GW FGQTF G QF
Sbjct: 302 NWGQNWQSNADLDGQSLSFRVTLSDGRTVTSNNAAPAGWSFGQTFEGGQF 451
>gb|BQ762357.1|BQ762357 EBro01_SQ005_H18_R root, 3 week, hydroponic grown, no treatment, cv
Optic, EBro01 Hordeum vulgare subsp. vulgare cDNA clone
EBro01_SQ005_H18 5', mRNA sequence
Length = 535
Score = 100 bits (213), Expect = 8e-021
Identities = 38/50 (76%), Positives = 42/50 (84%)
Frame = -3 / +1
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQF 499
+WGQNWQSN LDGQ+LSFRVT SDGR+V S+NAAP GW FGQTF G QF
Sbjct: 166 NWGQNWQSNADLDGQSLSFRVTLSDGRTVTSNNAAPAGWSFGQTFEGGQF 315
>gb|CA005047.1|CA005047 HU12E04u HU Hordeum vulgare subsp. vulgare cDNA clone HU12E04
3-PRIME, mRNA sequence
Length = 529
Score = 100 bits (213), Expect = 8e-021
Identities = 38/50 (76%), Positives = 42/50 (84%)
Frame = -3 / +3
Query: 648 HWGQNWQSNTLLDGQALSFRVTTSDGRSVVSSNAAPRGWGFGQTFSGAQF 499
+WGQNWQSN LDGQ+LSFRVT SDGR+V S+NAAP GW FGQTF G QF
Sbjct: 288 NWGQNWQSNADLDGQSLSFRVTLSDGRTVTSNNAAPAGWSFGQTFEGGQF 437
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 302,244,858
Number of Sequences: 312970
Number of extensions: 4990374
Number of successful extensions: 65069
Number of sequences better than 1.0e-020: 22
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7253257239
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)