TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2594147.2.1
(744 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ759881.1|BQ759881 EBpi05_SQ004_P16_R pistil, 8 DPA, no... 123 3e-046
gb|BE060246.3|BE060246 HVSMEg0011L08f Hordeum vulgare pre-a... 185 3e-046
gb|CK122346.1|CK122346 BES1824102p12 BES1824 Hordeum vulgar... 185 3e-046
gb|BF622288.3|BF622288 HVSMEa0002I16f Hordeum vulgare seedl... 122 3e-044
gb|BI950777.1|BI950777 HVSMEl0023C15f Hordeum vulgare spike... 113 4e-044
gb|BU980984.1|BU980984 HA22E18r HA Hordeum vulgare subsp. v... 154 4e-044
gb|BM098205.2|BM098205 EBpi03_SQ002_N14_R pistil, 4 DPA, no... 154 4e-044
gb|AV917139.1|AV917139 AV917139 K. Sato unpublished cDNA li... 117 1e-042
gb|CV058074.1|CV058074 BNEL34a7 Barley EST endosperm librar... 114 2e-041
gb|BG368109.2|BG368109 HVSMEi0015L12f Hordeum vulgare 20 DA... 152 4e-041
gb|BE454367.2|BE454367 HVSMEh0093N18f Hordeum vulgare 5-45 ... 167 7e-041
gb|CK123980.1|CK123980 BES1824106f16 BES1824 Hordeum vulgar... 117 2e-039
gb|BF260026.3|BF260026 HVSMEf0020O13f Hordeum vulgare seedl... 95 3e-038
gb|BJ463021.1|BJ463021 BJ463021 K. Sato unpublished cDNA li... 113 3e-038
gb|BF254126.2|BF254126 HVSMEf0003B13f Hordeum vulgare seedl... 117 4e-038
gb|BQ459628.1|BQ459628 HA08J01r HA Hordeum vulgare subsp. v... 137 8e-038
gb|BF257093.3|BF257093 HVSMEf0011N11f Hordeum vulgare seedl... 124 2e-037
gb|BM097618.2|BM097618 EBem04_SQ002_D15_R embryo, 12 DPA, n... 121 4e-037
gb|BG368126.2|BG368126 HVSMEi0015M21f Hordeum vulgare 20 DA... 154 5e-037
gb|BG368652.1|BG368652 HVSMEi0020B24f Hordeum vulgare 20 DA... 152 2e-036
gb|CV055940.1|CV055940 BNEL12B5 Barley EST endosperm librar... 93 3e-036
gb|BG369116.2|BG369116 HVSMEi0021M10f Hordeum vulgare 20 DA... 141 7e-036
gb|CK122737.1|CK122737 BES1824105j22 BES1824 Hordeum vulgar... 115 2e-035
gb|BI959138.1|BI959138 HVSMEn0018F10f Hordeum vulgare rachi... 126 3e-035
gb|CK124171.1|CK124171 BES1824107e17 BES1824 Hordeum vulgar... 143 1e-033
gb|BF253787.2|BF253787 HVSMEf0002C01f Hordeum vulgare seedl... 115 1e-033
gb|BQ465138.1|BQ465138 HU02M06r HU Hordeum vulgare subsp. v... 117 2e-033
gb|CA019924.1|CA019924 HV13L18r HV Hordeum vulgare subsp. v... 124 1e-032
gb|BQ459308.1|BQ459308 HA01I15r HA Hordeum vulgare subsp. v... 83 3e-032
gb|BI956772.1|BI956772 HVSMEn0005E06f Hordeum vulgare rachi... 118 6e-032
gb|AV928430.1|AV928430 AV928430 K. Sato unpublished cDNA li... 83 8e-032
gb|CV054003.1|CV054003 BNEL105f8 Barley EST endosperm libra... 116 3e-031
gb|BQ470849.1|BQ470849 HX04D14r HX Hordeum vulgare subsp. v... 81 5e-031
gb|CV063217.1|CV063217 BNEL87h3 Barley EST endosperm librar... 113 7e-031
gb|CV058907.1|CV058907 BNEL42c7 Barley EST endosperm librar... 133 1e-030
gb|CB881182.1|CB881182 HM09A07w HM Hordeum vulgare subsp. v... 67 2e-030
gb|CA017263.1|CA017263 HV14A15u HV Hordeum vulgare subsp. v... 75 7e-030
gb|CA019997.1|CA019997 HV14A15r HV Hordeum vulgare subsp. v... 117 1e-027
gb|BE193443.3|BE193443 HVSMEh0081A10f Hordeum vulgare 5-45 ... 105 1e-027
gb|AV917082.1|AV917082 AV917082 K. Sato unpublished cDNA li... 117 3e-027
gb|BU967134.1|BU967134 HB03G05r BC Hordeum vulgare subsp. v... 116 8e-026
gb|BM101447.2|BM101447 EBpi01_SQ003_O01_R pistil, 1 DPA, no... 75 1e-025
gb|BU981055.1|BU981055 HA22I03r HA Hordeum vulgare subsp. v... 116 1e-025
gb|CV063939.1|CV063939 BNEL94h5 Barley EST endosperm librar... 116 1e-025
gb|BE196421.3|BE196421 HVSMEh0092D24f Hordeum vulgare 5-45 ... 68 2e-025
gb|BI957603.1|BI957603 HVSMEn0010F11f Hordeum vulgare rachi... 114 5e-025
gb|BI958784.1|BI958784 HVSMEn0016J13f Hordeum vulgare rachi... 114 5e-025
gb|BQ458429.1|BQ458429 HA05E07r HA Hordeum vulgare subsp. v... 113 9e-025
gb|CB880652.1|CB880652 HM07E17w HM Hordeum vulgare subsp. v... 82 1e-024
gb|CB881234.1|CB881234 HM09C23w HM Hordeum vulgare subsp. v... 82 1e-024
gb|BI960458.1|BI960458 HVSMEn0024K16f Hordeum vulgare rachi... 110 1e-023
gb|BU995090.1|BU995090 HM09C23r HM Hordeum vulgare subsp. v... 110 1e-023
gb|BF628930.2|BF628930 HVSMEb0009C23f Hordeum vulgare seedl... 108 3e-023
gb|CB883447.1|CB883447 HQ02E17w HQ Hordeum vulgare subsp. v... 108 4e-023
gb|CV060611.1|CV060611 BNEL5B4 Barley EST endosperm library... 108 4e-023
gb|BQ467895.1|BQ467895 HR01E04r HR Hordeum vulgare subsp. v... 83 4e-023
gb|CV060955.1|CV060955 BNEL63c5 Barley EST endosperm librar... 91 1e-022
gb|BG416780.1|BG416780 HVSMEk0014H23f Hordeum vulgare testa... 105 4e-022
gb|AJ436682.1|AJ436682 AJ436682 S00002 Hordeum vulgare subs... 84 5e-022
gb|AJ463502.1|AJ463502 AJ463502 S00002 Hordeum vulgare subs... 84 5e-022
gb|BF256653.2|BF256653 HVSMEf0010J19f Hordeum vulgare seedl... 104 5e-022
gb|BF257559.2|BF257559 HVSMEf0013D22f Hordeum vulgare seedl... 98 2e-021
gb|BI959698.1|BI959698 HVSMEn0020N13f Hordeum vulgare rachi... 101 4e-021
gb|BM442635.2|BM442635 EBan01_SQ003_L19_R anther, yellow st... 63 8e-021
>gb|BQ759881.1|BQ759881 EBpi05_SQ004_P16_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ004_P16 5', mRNA sequence
Length = 500
Score = 123 bits (263), Expect(2) = 3e-046
Identities = 49/81 (60%), Positives = 60/81 (74%)
Frame = -1 / +2
Query: 711 HFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNYLAVLI 532
HFD+SGTAFGA+AKPG +LR +G I IQ+ RV CE+PG+ VTF VE GSNP Y AVL+
Sbjct: 5 HFDLSGTAFGAMAKPGLSEKLRHSGIIDIQFKRVPCEFPGLKVTFHVEQGSNPVYFAVLV 184
Query: 531 EYEDSDSALDSVDMMQTQGGT 469
EYED D + VD+M+ GT
Sbjct: 185 EYEDGDGDVVQVDLMEANSGT 247
Score = 82.2 bits (173), Expect(2) = 3e-046
Identities = 28/57 (49%), Positives = 40/57 (70%)
Frame = -1 / +2
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
+G W PM++SWG++W+++SG LQ PF++R+T SG+ LVA IPA W YRS
Sbjct: 239 SGTWTPMRESWGSIWRLDSGHRLQAPFSMRITNESGKTLVADKVIPANWAPSTFYRS 409
Score = 72.1 bits (151), Expect(2) = 6e-023
Identities = 37/73 (50%), Positives = 42/73 (57%)
Frame = +1 / -2
Query: 493 VHGVEGAVAILVLDQHGQVVRVGARLHREGHVHARPLALHPGILDLDGAGATQLALLPGL 672
VH AVA+LVL QHG+V RV LH EGH+ R LA H LD+D AG +L GL
Sbjct: 223 VHLHHVAVAVLVLHQHGEVHRVRPLLHVEGHLETRELARHSLELDVDDAGVPELLAEAGL 44
Query: 673 RQGTERCPAHVEV 711
G ER VEV
Sbjct: 43 GHGAERGATQVEV 5
Score = 55.1 bits (114), Expect(2) = 6e-023
Identities = 25/46 (54%), Positives = 26/46 (56%)
Frame = +2 / -3
Query: 329 AGMALLATSALPELKVRRMLKGPWRTEPELIFHTAPHDCCIGTHCP 466
AGM L ATS LP+ V RM KG WR PE PHD IG H P
Sbjct: 378 AGMTLSATSVLPDSLVMRMEKGAWRRWPESSRQIDPHDSRIGVHVP 241
>gb|BE060246.3|BE060246 HVSMEg0011L08f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011L08f, mRNA sequence
Length = 544
Score = 185 bits (398), Expect = 3e-046
Identities = 73/143 (51%), Positives = 98/143 (68%)
Frame = -1 / +2
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P HFD+SGTAFG LAKPG +LR +G I I++TRV CE+PG+ + F VE SNP Y
Sbjct: 17 PVAPYHFDLSGTAFGRLAKPGLNDRLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVY 196
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGTTGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFS 367
AVL+EYED D + VD+M+++G G+W M++SWG+VW+++S LQ PF+IR+
Sbjct: 197 FAVLVEYEDGDGDVVQVDLMESRGPGGGKWTRMRESWGSVWRLDSNHRLQAPFSIRIRNE 376
Query: 366 SGRALVASNAIPAGWNAGVAYRS 298
SG+ LVA+ IPA W YRS
Sbjct: 377 SGKTLVANKVIPANWRPNTFYRS 445
>gb|CK122346.1|CK122346 BES1824102p12 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P122 5-PRIME, mRNA sequence
Length = 717
Score = 185 bits (398), Expect = 3e-046
Identities = 73/143 (51%), Positives = 98/143 (68%)
Frame = -1 / +1
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P HFD+SGTAFG LAKPG +LR +G I I++TRV CE+PG+ + F VE SNP Y
Sbjct: 145 PVAPYHFDLSGTAFGRLAKPGLNDRLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVY 324
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGTTGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFS 367
AVL+EYED D + VD+M+++G G+W M++SWG+VW+++S LQ PF+IR+
Sbjct: 325 FAVLVEYEDGDGDVVQVDLMESRGPGGGKWTRMRESWGSVWRLDSNHRLQAPFSIRIRNE 504
Query: 366 SGRALVASNAIPAGWNAGVAYRS 298
SG+ LVA+ IPA W YRS
Sbjct: 505 SGKTLVANKVIPANWRPNTFYRS 573
>gb|BF622288.3|BF622288 HVSMEa0002I16f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0002I16f, mRNA sequence
Length = 851
Score = 122 bits (262), Expect(2) = 3e-044
Identities = 47/83 (56%), Positives = 64/83 (77%)
Frame = -1 / +2
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P + HFD+SGTAFGA+AK G+ +LR AG I +Q+ RV C++PG+ VTF V+ GSNP Y
Sbjct: 287 PVSRFHFDLSGTAFGAMAKDGRNEELRHAGIIDMQFRRVPCQYPGLTVTFHVQHGSNPYY 466
Query: 546 LAVLIEYEDSDSALDSVDMMQTQ 478
LA+L+EYE+ D +D VDMMQ++
Sbjct: 467 LAILVEYENGDGDVDQVDMMQSR 535
Score = 75.8 bits (159), Expect(2) = 3e-044
Identities = 25/57 (43%), Positives = 38/57 (66%)
Frame = -1 / +2
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
TG+WVPM +SWG++W++++ +QGPF++R+T SG+ LVA P W Y S
Sbjct: 572 TGEWVPMTESWGSIWRMDTRRPMQGPFSLRITNESGKTLVADQVNPGDWEPNEIYSS 742
>gb|BI950777.1|BI950777 HVSMEl0023C15f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023C15f, mRNA sequence
Length = 986
Score = 113 bits (242), Expect(2) = 4e-044
Identities = 45/89 (50%), Positives = 58/89 (65%)
Frame = -1 / +3
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P E HFDMSGTA GA+AKPG +LR G I++QY RV C++PGV++ F V+ GSNP Y
Sbjct: 153 PGEPCHFDMSGTALGAMAKPGMADKLRAGGVIRMQYKRVPCKYPGVNIAFKVDQGSNPFY 332
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGTTGQ 460
LIE+ED D L +V + + G Q
Sbjct: 333 FKTLIEFEDDDGDLKAVALKEAGSGAWTQ 419
Score = 84.5 bits (178), Expect(2) = 4e-044
Identities = 30/57 (52%), Positives = 41/57 (71%)
Frame = -1 / +3
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
+G W M Q WGA+W++N+G+ L+ PF+++LT SGR LV +N I A WNAG YRS
Sbjct: 402 SGAWTQMTQDWGALWRLNNGNRLRAPFSLQLTSDSGRKLVINNVILANWNAGATYRS 572
Score = 76.7 bits (161), Expect(2) = 2e-022
Identities = 35/63 (55%), Positives = 39/63 (61%)
Frame = +2 / -2
Query: 554 GLEPGSTEKVTSTPGHSHCTRVYWIWMAPAPRSWLSCPGFAKAPNAVPLMSKWTCSSGHG 733
GLEP ST K TPG+ H T +Y I M P RS + PGFA AP AVPLMSKW S G+
Sbjct: 325 GLEPWSTLKAMLTPGYLHGTLLYCILMTPPARSLSAMPGFAMAPKAVPLMSKWHGSPGYR 146
Query: 734 QSG 742
G
Sbjct: 145 LPG 137
Score = 48.7 bits (100), Expect(2) = 2e-022
Identities = 25/57 (43%), Positives = 28/57 (49%)
Frame = +2 / -2
Query: 299 DRYATPAFHPAGMALLATSALPELKVRRMLKGPWRTEPELIFHTAPHDCCIGTHCPV 469
+RY PAF A M LL TS LPE +V KG P L H AP C I P+
Sbjct: 571 ERYVAPAFQLARMTLLMTSFLPESEVSWSEKGARSRLPLLRRHNAPQSCVICVQAPL 401
>gb|BU980984.1|BU980984 HA22E18r HA Hordeum vulgare subsp. vulgare cDNA clone HA22E18
5-PRIME, mRNA sequence
Length = 567
Score = 154 bits (331), Expect = 5e-037
Identities = 61/94 (64%), Positives = 69/94 (73%)
Frame = -1 / +3
Query: 744 CPDCPCPDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEP 565
CPD PC DE VHFD+SGTAFG +AKPGQ QLR AG +QIQYTRV C W G+ V F V+
Sbjct: 39 CPDGPCLDEPVHFDLSGTAFGGMAKPGQADQLRAAGRLQIQYTRVPCNWRGMHVAFKVDA 218
Query: 564 GSNPNYLAVLIEYEDSDSALDSVDMMQTQGGTTG 463
GSNP YLAVLIEYED D L SV++M+ G G
Sbjct: 219 GSNPYYLAVLIEYEDGDGDLSSVELMENGGNGAG 320
Score = 103 bits (219), Expect(2) = 1e-036
Identities = 42/63 (66%), Positives = 43/63 (68%)
Frame = +2 / -3
Query: 554 GLEPGSTEKVTSTPGHSHCTRVYWIWMAPAPRSWLSCPGFAKAPNAVPLMSKWTCSSGHG 733
GLEP ST K T P H TRVYWIW PA RSW + PGFA PNAVPL SKWT SS HG
Sbjct: 229 GLEPASTLKATCIPRQLHGTRVYWIWRRPAARSWSAWPGFAMPPNAVPLRSKWTGSSRHG 50
Query: 734 QSG 742
SG
Sbjct: 49 PSG 41
Score = 102 bits (217), Expect(2) = 4e-044
Identities = 49/84 (58%), Positives = 53/84 (63%)
Frame = -2 / +1
Query: 743 APIARVLMSRSTST*AGQRSVPWRSPGRRASCVAPAPSKSSIPGCSASGLAWT*PSRWSR 564
AP R MS STST*AG+RS WRSP R S P SKSS G A+G T*PSRW+
Sbjct: 40 APTGRAWMSPSTST*AGRRSAAWRSPARPTSFARPDVSKSSTHGSRATGGGCT*PSRWTP 219
Query: 563 APTRTTWPC*SSTRIATAPSTPWT 492
APT TT PC SSTR ATA W+
Sbjct: 220 APTPTTSPCSSSTRTATATCRRWS 291
Score = 101 bits (215), Expect(2) = 3e-023
Identities = 45/84 (53%), Positives = 59/84 (70%)
Frame = +1 / -2
Query: 493 VHGVEGAVAILVLDQHGQVVRVGARLHREGHVHARPLALHPGILDLDGAGATQLALLPGL 672
+H + AVA+LVLD+HG+VV VGA +H EG+VH P+A P +LDL+ +G +L L GL
Sbjct: 290 LHRRQVAVAVLVLDEHGEVVGVGAGVHLEGYVHPPPVARDPCVLDLETSGRAKLVGLAGL 111
Query: 673 RQGTERCPAHVEVDLLIRTRAIGA 744
R ER PA VEVD LI+ R +GA
Sbjct: 110 RHAAERRPAQVEVDGLIQARPVGA 39
Score = 95.9 bits (203), Expect(2) = 4e-044
Identities = 36/64 (56%), Positives = 43/64 (67%)
Frame = -1 / +3
Query: 489 MQTQGGTTGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGV 310
+ GG W M +SWGAVW+ NSG +L PF++RLT SSG+ LVASN IPAGW G
Sbjct: 291 LMENGGNGAGWTKMDRSWGAVWRYNSGPMLHAPFSVRLTSSSGKTLVASNVIPAGWKPGG 470
Query: 309 AYRS 298
YRS
Sbjct: 471 TYRS 482
Score = 70.3 bits (147), Expect(2) = 1e-036
Identities = 32/60 (53%), Positives = 35/60 (58%)
Frame = +2 / -3
Query: 299 DRYATPAFHPAGMALLATSALPELKVRRMLKGPWRTEPELIFHTAPHDCCIGTHCPVVPP 478
+R P F PAGM LLAT LPEL+VRR KG PEL HTAPHD I +PP
Sbjct: 481 ER*VPPGFQPAGMTLLATRVLPELEVRRTEKGACNIGPELYLHTAPHDRSILVQPAPLPP 302
Score = 26.7 bits (52), Expect(2) = 3e-023
Identities = 15/35 (42%), Positives = 18/35 (51%)
Frame = +1 / -2
Query: 313 AGVPPRRYGVAGDERPARAEGEADVEGALEDGAGV 417
AG+P V E A A GEAD EG ++ GV
Sbjct: 467 AGLPAGGDDVTCHESLAGAGGEADREGRMQHWPGV 363
>gb|BM098205.2|BM098205 EBpi03_SQ002_N14_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ002_N14 5', mRNA sequence
Length = 550
Score = 154 bits (331), Expect = 5e-037
Identities = 61/94 (64%), Positives = 69/94 (73%)
Frame = -1 / +1
Query: 744 CPDCPCPDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEP 565
CPD PC DE VHFD+SGTAFG +AKPGQ QLR AG +QIQYTRV C W G+ V F V+
Sbjct: 25 CPDGPCLDEPVHFDLSGTAFGGMAKPGQADQLRAAGRLQIQYTRVPCNWRGMHVAFKVDA 204
Query: 564 GSNPNYLAVLIEYEDSDSALDSVDMMQTQGGTTG 463
GSNP YLAVLIEYED D L SV++M+ G G
Sbjct: 205 GSNPYYLAVLIEYEDGDGDLSSVELMENGGNGAG 306
Score = 103 bits (219), Expect(2) = 1e-036
Identities = 42/63 (66%), Positives = 43/63 (68%)
Frame = +2 / -3
Query: 554 GLEPGSTEKVTSTPGHSHCTRVYWIWMAPAPRSWLSCPGFAKAPNAVPLMSKWTCSSGHG 733
GLEP ST K T P H TRVYWIW PA RSW + PGFA PNAVPL SKWT SS HG
Sbjct: 215 GLEPASTLKATCIPRQLHGTRVYWIWRRPAARSWSAWPGFAMPPNAVPLRSKWTGSSRHG 36
Query: 734 QSG 742
SG
Sbjct: 35 PSG 27
Score = 102 bits (217), Expect(2) = 4e-044
Identities = 49/84 (58%), Positives = 53/84 (63%)
Frame = -2 / +2
Query: 743 APIARVLMSRSTST*AGQRSVPWRSPGRRASCVAPAPSKSSIPGCSASGLAWT*PSRWSR 564
AP R MS STST*AG+RS WRSP R S P SKSS G A+G T*PSRW+
Sbjct: 26 APTGRAWMSPSTST*AGRRSAAWRSPARPTSFARPDVSKSSTHGSRATGGGCT*PSRWTP 205
Query: 563 APTRTTWPC*SSTRIATAPSTPWT 492
APT TT PC SSTR ATA W+
Sbjct: 206 APTPTTSPCSSSTRTATATCRRWS 277
Score = 101 bits (215), Expect = 5e-021
Identities = 45/84 (53%), Positives = 59/84 (70%)
Frame = +1 / -2
Query: 493 VHGVEGAVAILVLDQHGQVVRVGARLHREGHVHARPLALHPGILDLDGAGATQLALLPGL 672
+H + AVA+LVLD+HG+VV VGA +H EG+VH P+A P +LDL+ +G +L L GL
Sbjct: 276 LHRRQVAVAVLVLDEHGEVVGVGAGVHLEGYVHPPPVARDPCVLDLETSGRAKLVGLAGL 97
Query: 673 RQGTERCPAHVEVDLLIRTRAIGA 744
R ER PA VEVD LI+ R +GA
Sbjct: 96 RHAAERRPAQVEVDGLIQARPVGA 25
Score = 95.9 bits (203), Expect(2) = 4e-044
Identities = 36/64 (56%), Positives = 43/64 (67%)
Frame = -1 / +1
Query: 489 MQTQGGTTGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGV 310
+ GG W M +SWGAVW+ NSG +L PF++RLT SSG+ LVASN IPAGW G
Sbjct: 277 LMENGGNGAGWTKMDRSWGAVWRYNSGPMLHAPFSVRLTSSSGKTLVASNVIPAGWKPGG 456
Query: 309 AYRS 298
YRS
Sbjct: 457 TYRS 468
Score = 70.3 bits (147), Expect(2) = 1e-036
Identities = 32/60 (53%), Positives = 35/60 (58%)
Frame = +2 / -3
Query: 299 DRYATPAFHPAGMALLATSALPELKVRRMLKGPWRTEPELIFHTAPHDCCIGTHCPVVPP 478
+R P F PAGM LLAT LPEL+VRR KG PEL HTAPHD I +PP
Sbjct: 467 ER*VPPGFQPAGMTLLATRVLPELEVRRTEKGACNIGPELYLHTAPHDRSILVQPAPLPP 288
>gb|AV917139.1|AV917139 AV917139 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20o17 5', mRNA sequence
Length = 590
Score = 117 bits (251), Expect(2) = 1e-042
Identities = 48/88 (54%), Positives = 62/88 (70%)
Frame = -1 / +1
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P HFD+SGTAFG LAKPG+ +LR AG I IQ+TRV CE+PG+ V F VE GSN Y
Sbjct: 67 PVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFPGLKVGFHVEEGSNAVY 246
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGTTG 463
+A+L+EYE+ D + VD+M++ G G
Sbjct: 247 MAILVEYENGDGDVVQVDLMESGRGRRG 330
Score = 75.3 bits (158), Expect(2) = 1e-042
Identities = 26/56 (46%), Positives = 37/56 (66%)
Frame = -1 / +1
Query: 465 GQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
G+W M++SWG++W+++S LQ PF+IR+ SG+ LVA N IP W YRS
Sbjct: 334 GRWTRMRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIPKNWRPNTFYRS 501
>gb|CV058074.1|CV058074 BNEL34a7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL34a7 5' similar to Oryza sativa
beta-expansin, mRNA sequence
Length = 630
Score = 114 bits (244), Expect(2) = 2e-041
Identities = 43/80 (53%), Positives = 62/80 (77%)
Frame = -1 / +3
Query: 711 HFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNYLAVLI 532
HFD+SGTAFGA+AK G+ +LR AG I +Q+ RV C++PG+ VTF VE GSNP Y+A+L+
Sbjct: 3 HFDLSGTAFGAMAKYGRNDELRHAGIINMQFKRVPCQYPGLTVTFHVEEGSNPFYMAILV 182
Query: 531 EYEDSDSALDSVDMMQTQGG 472
EYE+ D + +D+M+++ G
Sbjct: 183 EYENGDGDVKQLDIMESRPG 242
Score = 74.8 bits (157), Expect(2) = 2e-041
Identities = 25/57 (43%), Positives = 38/57 (66%)
Frame = -1 / +3
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
TGQWVPM++SWG++W++++ + GPF++R+T SG+ LVA I W Y S
Sbjct: 270 TGQWVPMKESWGSIWRMDAHHPMHGPFSLRVTNESGKTLVADQVIXGDWKPNKTYSS 440
>gb|BG368109.2|BG368109 HVSMEi0015L12f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0015L12f, mRNA sequence
Length = 871
Score = 152 bits (327), Expect(2) = 4e-041
Identities = 60/90 (66%), Positives = 68/90 (75%)
Frame = -1 / +1
Query: 744 CPDCPCPDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEP 565
CPD PC DE VHFD+SGTAFG +AKPGQ QLR AG +QIQYTRV C W G+ V F V+
Sbjct: 253 CPDGPCLDEPVHFDLSGTAFGGMAKPGQADQLRAAGRLQIQYTRVPCNWRGMHVAFKVDA 432
Query: 564 GSNPNYLAVLIEYEDSDSALDSVDMMQTQG 475
GSNP YLAVLIEYED D L SV++M+ G
Sbjct: 433 GSNPYYLAVLIEYEDGDGDLSSVELMENGG 522
Score = 103 bits (219), Expect = 1e-021
Identities = 42/63 (66%), Positives = 43/63 (68%)
Frame = +2 / -3
Query: 554 GLEPGSTEKVTSTPGHSHCTRVYWIWMAPAPRSWLSCPGFAKAPNAVPLMSKWTCSSGHG 733
GLEP ST K T P H TRVYWIW PA RSW + PGFA PNAVPL SKWT SS HG
Sbjct: 443 GLEPASTLKATCIPRQLHGTRVYWIWRRPAARSWSAWPGFAMPPNAVPLRSKWTGSSRHG 264
Query: 734 QSG 742
SG
Sbjct: 263 PSG 255
Score = 102 bits (217), Expect = 3e-021
Identities = 49/84 (58%), Positives = 53/84 (63%)
Frame = -2 / +2
Query: 743 APIARVLMSRSTST*AGQRSVPWRSPGRRASCVAPAPSKSSIPGCSASGLAWT*PSRWSR 564
AP R MS STST*AG+RS WRSP R S P SKSS G A+G T*PSRW+
Sbjct: 254 APTGRAWMSPSTST*AGRRSAAWRSPARPTSFARPDVSKSSTHGSRATGGGCT*PSRWTP 433
Query: 563 APTRTTWPC*SSTRIATAPSTPWT 492
APT TT PC SSTR ATA W+
Sbjct: 434 APTPTTSPCSSSTRTATATCPRWS 505
Score = 101 bits (215), Expect = 5e-021
Identities = 45/84 (53%), Positives = 59/84 (70%)
Frame = +1 / -2
Query: 493 VHGVEGAVAILVLDQHGQVVRVGARLHREGHVHARPLALHPGILDLDGAGATQLALLPGL 672
+H + AVA+LVLD+HG+VV VGA +H EG+VH P+A P +LDL+ +G +L L GL
Sbjct: 504 LHRGQVAVAVLVLDEHGEVVGVGAGVHLEGYVHPPPVARDPCVLDLETSGRAKLVGLAGL 325
Query: 673 RQGTERCPAHVEVDLLIRTRAIGA 744
R ER PA VEVD LI+ R +GA
Sbjct: 324 RHAAERRPAQVEVDGLIQARPVGA 253
Score = 35.4 bits (71), Expect(2) = 4e-041
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = -1 / +3
Query: 444 QSWGAVWKINSGSVLQGPF 388
+SWGAVW++NS +L PF
Sbjct: 552 RSWGAVWRVNSRPMLHAPF 608
>gb|BE454367.2|BE454367 HVSMEh0093N18f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0093N18f, mRNA sequence
Length = 870
Score = 167 bits (359), Expect = 7e-041
Identities = 67/142 (47%), Positives = 90/142 (63%)
Frame = -1 / +1
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P HFD+SGTAFG LAKPG +LR +G I I++TRV CE+PG+ + F VE SNP Y
Sbjct: 28 PVAPYHFDLSGTAFGRLAKPGLNDRLRHSGIIDIEFTRVPCEFPGLKIGFHVEEYSNPVY 207
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGTTGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFS 367
AVL+EYED D + VD+M+++G G+W M++SWG+VW++N LQ PF I++
Sbjct: 208 FAVLVEYEDGDGDVVQVDLMESRGPGGGKWTRMRESWGSVWRLNCNHRLQAPFCIQVRDK 387
Query: 366 SGRALVASNAIPAGWNAGVAYR 301
G L S +P W G R
Sbjct: 388 CGETLGGSMGVPGRWRPGTLCR 453
>gb|CK123980.1|CK123980 BES1824106f16 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010F166 5-PRIME, mRNA sequence
Length = 750
Score = 117 bits (251), Expect(2) = 2e-039
Identities = 48/88 (54%), Positives = 62/88 (70%)
Frame = -1 / +1
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P HFD+SGTAFG LAKPG+ +LR AG I IQ+TRV CE+PG+ V F VE GSN Y
Sbjct: 262 PVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFPGLKVGFHVEEGSNAVY 441
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGTTG 463
+A+L+EYE+ D + VD+M++ G G
Sbjct: 442 MAILVEYENGDGDVVQVDLMESGRGRRG 525
Score = 64.8 bits (135), Expect(2) = 2e-039
Identities = 22/45 (48%), Positives = 33/45 (73%)
Frame = -1 / +1
Query: 465 GQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIP 331
G+W M++SWG++W+++S LQ PF+IR+ SG+ LVA N IP
Sbjct: 529 GRWTRMRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIP 663
>gb|BF260026.3|BF260026 HVSMEf0020O13f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020O13f, mRNA sequence
Length = 535
Score = 95.5 bits (202), Expect(2) = 3e-038
Identities = 41/76 (53%), Positives = 50/76 (65%)
Frame = -1 / +1
Query: 696 GTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNYLAVLIEYEDS 517
GTAFGALAK G +LR +G I IQ+ RV C +PG+ + F V GSN YLAVLIEYED
Sbjct: 1 GTAFGALAKSGLNEKLRHSGIIDIQFRRVPCNFPGLKINFHVVEGSNAVYLAVLIEYEDM 180
Query: 516 DSALDSVDMMQTQGGT 469
D + VDM + G+
Sbjct: 181 DGDVIQVDMKEANSGS 228
Score = 83.1 bits (175), Expect(2) = 3e-038
Identities = 28/57 (49%), Positives = 42/57 (73%)
Frame = -1 / +1
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
+G W+ M++SWG++W+++S LQGPF++R+T SG+ LVA+N IPA W YRS
Sbjct: 220 SGSWMAMRESWGSIWRMDSNHRLQGPFSMRITSDSGKKLVANNVIPANWRPNTDYRS 390
>gb|BJ463021.1|BJ463021 BJ463021 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags25g15 5', mRNA sequence
Length = 532
Score = 113 bits (241), Expect(2) = 3e-038
Identities = 45/89 (50%), Positives = 58/89 (65%)
Frame = -1 / +3
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P E HFDMSGTA GA+AKPG +LR G I++QY RV C++PGV++ F V+ GSNP Y
Sbjct: 147 PGEPCHFDMSGTALGAMAKPGMADKLRAGGVIRMQYKRVPCKYPGVNIAFRVDQGSNPFY 326
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGTTGQ 460
LIE+ED D L +V + + G Q
Sbjct: 327 FKTLIEFEDDDGDLKAVALKEAGSGAWTQ 413
Score = 65.2 bits (136), Expect(2) = 3e-038
Identities = 22/43 (51%), Positives = 32/43 (74%)
Frame = -1 / +3
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASN 340
+G W M Q WGA+W++N+G+ L+ PF++RLT SGR LV +N
Sbjct: 396 SGAWTQMTQDWGALWRLNNGNRLRAPFSLRLTXDSGRKLVXNN 524
>gb|BF254126.2|BF254126 HVSMEf0003B13f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0003B13f, mRNA sequence
Length = 789
Score = 117 bits (251), Expect(2) = 4e-038
Identities = 48/88 (54%), Positives = 62/88 (70%)
Frame = -1 / +2
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P HFD+SGTAFG LAKPG+ +LR AG I IQ+TRV CE+PG+ V F VE GSN Y
Sbjct: 206 PVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFPGLKVGFHVEEGSNAVY 385
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGTTG 463
+A+L+EYE+ D + VD+M++ G G
Sbjct: 386 MAILVEYENGDGDVVQVDLMESGRGRRG 469
Score = 60.2 bits (125), Expect(2) = 4e-038
Identities = 24/61 (39%), Positives = 35/61 (57%)
Frame = -1 / +2
Query: 465 GQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRSGGVA 286
G+W M++SWG +W+++S LQ PF+IR+ SG+ LVA IP Y S +
Sbjct: 473 GRWTRMRESWGFIWRLDSNHRLQAPFSIRIRNESGQTLVAR*VIPKKGRPNTLYCSSCIP 652
Query: 285 V 283
V
Sbjct: 653 V 655
>gb|BQ459628.1|BQ459628 HA08J01r HA Hordeum vulgare subsp. vulgare cDNA clone HA08J01
5-PRIME, mRNA sequence
Length = 470
Score = 137 bits (294), Expect(2) = 8e-038
Identities = 53/107 (49%), Positives = 73/107 (68%)
Frame = -1 / +1
Query: 618 TRVQCEWPGVDVTFSVEPGSNPNYLAVLIEYEDSDSALDSVDMMQTQGGTTGQWVPMQQS 439
TRV CE+PG+ + F VE SNP Y AVL+EYED D + VD+M+++G G+W M++S
Sbjct: 85 TRVPCEFPGLKIGFHVEEYSNPVYFAVLVEYEDGDGDVVQVDLMESRGPGGGKWTRMRES 264
Query: 438 WGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
WG+VW+++S LQ PF+IR+ SG+ LVA+ IPA W YRS
Sbjct: 265 WGSVWRLDSNHRLQAPFSIRIRNESGKTLVANKVIPANWRPNTFYRS 405
Score = 39.6 bits (80), Expect(2) = 8e-038
Identities = 16/27 (59%), Positives = 20/27 (74%)
Frame = -1 / +2
Query: 702 MSGTAFGALAKPGQESQLRGAGAIQIQ 622
+SGTAFG LAKPG +LR +G I I+
Sbjct: 2 LSGTAFGRLAKPGLNDRLRHSGIIDIE 82
>gb|BF257093.3|BF257093 HVSMEf0011N11f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0011N11f, mRNA sequence
Length = 816
Score = 124 bits (265), Expect(4) = 2e-037
Identities = 47/87 (54%), Positives = 64/87 (73%)
Frame = -1 / +2
Query: 729 CPDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPN 550
C E HFDMSGT+ GA+AKPG +LR +G ++IQY RV C++PG+++ F V+ GSNP
Sbjct: 200 CLGEAAHFDMSGTSMGAMAKPGMADKLRASGIVKIQYKRVPCKYPGMNIAFKVDQGSNPF 379
Query: 549 YLAVLIEYEDSDSALDSVDMMQTQGGT 469
YL VLIE+ED D L++VD+M+ GT
Sbjct: 380 YLEVLIEFEDDDGDLNTVDLMEANCGT 460
Score = 35.4 bits (71), Expect(4) = 2e-037
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = -1 / +2
Query: 465 GQWVPMQQSWGAVWKINSGS 406
G W PM Q+WGA++++NS +
Sbjct: 455 GTWTPMVQNWGALYRLNSNT 514
Score = 26.3 bits (51), Expect(4) = 2e-037
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = -1 / +2
Query: 417 NSGSVLQGPFNIRLTFSS 364
N+G L+GPF++R+T S
Sbjct: 509 NTGKPLRGPFSLRVTSDS 562
Score = 25.8 bits (50), Expect(4) = 2e-037
Identities = 8/15 (53%), Positives = 8/15 (53%)
Frame = -1 / +1
Query: 342 NAIPAGWNAGVAYRS 298
N P W AG YRS
Sbjct: 586 NVFPVSWKAGATYRS 630
>gb|BM097618.2|BM097618 EBem04_SQ002_D15_R embryo, 12 DPA, no treatment, cv Optic, EBem04
Hordeum vulgare subsp. vulgare cDNA clone
EBem04_SQ002_D15 5', mRNA sequence
Length = 477
Score = 121 bits (258), Expect(2) = 4e-037
Identities = 46/83 (55%), Positives = 64/83 (77%)
Frame = -1 / +3
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P + HFD+SGTAFGA+AK G+ +LR AG I +Q+ RV C++PG+ VTF V+ GSNPNY
Sbjct: 111 PVSRYHFDLSGTAFGAMAKYGRNDELRHAGIIDMQFKRVPCQYPGLTVTFRVQHGSNPNY 290
Query: 546 LAVLIEYEDSDSALDSVDMMQTQ 478
LA+L+EYED D + VD+M+++
Sbjct: 291 LAILVEYEDGDGDVAQVDIMESR 359
Score = 53.8 bits (111), Expect(2) = 4e-037
Identities = 16/32 (50%), Positives = 26/32 (81%)
Frame = -1 / +3
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLT 373
TG W PM++SWG++W+++ LQGPF++R+T
Sbjct: 378 TGYWTPMRESWGSIWRLDRLRPLQGPFSLRVT 473
>gb|BG368126.2|BG368126 HVSMEi0015M21f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0015M21f, mRNA sequence
Length = 817
Score = 154 bits (331), Expect = 5e-037
Identities = 61/94 (64%), Positives = 69/94 (73%)
Frame = -1 / +3
Query: 744 CPDCPCPDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEP 565
CPD PC DE VHFD+SGTAFG +AKPGQ QLR AG +QIQYTRV C W G+ V F V+
Sbjct: 393 CPDGPCLDEPVHFDLSGTAFGGMAKPGQADQLRAAGRLQIQYTRVPCNWRGMHVAFKVDA 572
Query: 564 GSNPNYLAVLIEYEDSDSALDSVDMMQTQGGTTG 463
GSNP YLAVLIEYED D L SV++M+ G G
Sbjct: 573 GSNPYYLAVLIEYEDGDGDLSSVELMENGGNGAG 674
Score = 103 bits (219), Expect = 1e-021
Identities = 42/63 (66%), Positives = 43/63 (68%)
Frame = +2 / -1
Query: 554 GLEPGSTEKVTSTPGHSHCTRVYWIWMAPAPRSWLSCPGFAKAPNAVPLMSKWTCSSGHG 733
GLEP ST K T P H TRVYWIW PA RSW + PGFA PNAVPL SKWT SS HG
Sbjct: 583 GLEPASTLKATCIPRQLHGTRVYWIWRRPAARSWSAWPGFAMPPNAVPLRSKWTGSSRHG 404
Query: 734 QSG 742
SG
Sbjct: 403 PSG 395
Score = 99.6 bits (211), Expect(2) = 2e-023
Identities = 48/84 (57%), Positives = 52/84 (61%)
Frame = -2 / +1
Query: 743 APIARVLMSRSTST*AGQRSVPWRSPGRRASCVAPAPSKSSIPGCSASGLAWT*PSRWSR 564
AP R MS STST*AG+RS WRSP R S P SKSS G A+G T*PSRW+
Sbjct: 394 APTGRAWMSPSTST*AGRRSAAWRSPARPTSFARPDVSKSSTHGSRATGGGCT*PSRWTP 573
Query: 563 APTRTTWPC*SSTRIATAPSTPWT 492
APT TT PC STR ATA W+
Sbjct: 574 APTPTTSPCSLSTRTATATCRRWS 645
Score = 29.0 bits (57), Expect(2) = 2e-023
Identities = 15/53 (28%), Positives = 23/53 (43%)
Frame = -1 / +3
Query: 489 MQTQGGTTGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIP 331
+ GG W M S G ++ + + PF RLT S G+ L+ + P
Sbjct: 645 LMENGGNGAGWTXMDPSLGRGVEVQLRANVDAPFLGRLTCSFGKILLEVTSSP 803
>gb|BG368652.1|BG368652 HVSMEi0020B24f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0020B24f, mRNA sequence
Length = 679
Score = 152 bits (326), Expect = 2e-036
Identities = 60/94 (63%), Positives = 68/94 (72%)
Frame = -1 / +1
Query: 744 CPDCPCPDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEP 565
CPD PC DE VHFD+SGTAFG +AKPGQ QLR AG +QIQYTR C W G+ V F V+
Sbjct: 343 CPDGPCLDEPVHFDLSGTAFGGMAKPGQADQLRAAGRLQIQYTRFPCNWRGMHVAFKVDA 522
Query: 564 GSNPNYLAVLIEYEDSDSALDSVDMMQTQGGTTG 463
GSNP YLAVLIEYED D L SV++M+ G G
Sbjct: 523 GSNPYYLAVLIEYEDGDGDLSSVELMENGGNGAG 624
Score = 102 bits (217), Expect(2) = 2e-026
Identities = 49/84 (58%), Positives = 53/84 (63%)
Frame = -2 / +2
Query: 743 APIARVLMSRSTST*AGQRSVPWRSPGRRASCVAPAPSKSSIPGCSASGLAWT*PSRWSR 564
AP R MS STST*AG+RS WRSP R S P SKSS G A+G T*PSRW+
Sbjct: 344 APTGRAWMSPSTST*AGRRSAAWRSPARPTSFARPDVSKSSTHGSRATGGGCT*PSRWTP 523
Query: 563 APTRTTWPC*SSTRIATAPSTPWT 492
APT TT PC SSTR ATA W+
Sbjct: 524 APTPTTSPCSSSTRTATATCRRWS 595
Score = 101 bits (216), Expect(2) = 4e-022
Identities = 45/84 (53%), Positives = 59/84 (70%)
Frame = +1 / -2
Query: 493 VHGVEGAVAILVLDQHGQVVRVGARLHREGHVHARPLALHPGILDLDGAGATQLALLPGL 672
+H + AVA+LVLD+HG+VV VGA +H EG+VH P+A P +LDL+ +G +L L GL
Sbjct: 594 LHRRQVAVAVLVLDEHGEVVGVGAGVHLEGYVHPPPVAREPCVLDLETSGRAKLVGLAGL 415
Query: 673 RQGTERCPAHVEVDLLIRTRAIGA 744
R ER PA VEVD LI+ R +GA
Sbjct: 414 RHAAERRPAQVEVDGLIQARPVGA 343
Score = 100 bits (214), Expect = 7e-021
Identities = 41/63 (65%), Positives = 42/63 (66%)
Frame = +2 / -3
Query: 554 GLEPGSTEKVTSTPGHSHCTRVYWIWMAPAPRSWLSCPGFAKAPNAVPLMSKWTCSSGHG 733
GLEP ST K T P H RVYWIW PA RSW + PGFA PNAVPL SKWT SS HG
Sbjct: 533 GLEPASTLKATCIPRQLHGNRVYWIWRRPAARSWSAWPGFAMPPNAVPLRSKWTGSSRHG 354
Query: 734 QSG 742
SG
Sbjct: 353 PSG 345
Score = 36.8 bits (74), Expect(2) = 2e-026
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = -1 / +1
Query: 489 MQTQGGTTGQWVPMQQSWGAVWKINS 412
+ GG W M SWGAVW+ NS
Sbjct: 595 LMENGGNGAGWTKMDPSWGAVWRYNS 672
Score = 22.6 bits (43), Expect(2) = 4e-022
Identities = 10/22 (45%), Positives = 11/22 (50%)
Frame = +2 / -3
Query: 413 ELIFHTAPHDCCIGTHCPVVPP 478
EL HTAP D I +PP
Sbjct: 671 ELYLHTAPQDGSILVQPAPLPP 606
>gb|CV055940.1|CV055940 BNEL12B5 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL12B5 5' similar to Oryza sativa
beta-expansin, mRNA sequence
Length = 614
Score = 93.2 bits (197), Expect(2) = 3e-036
Identities = 35/71 (49%), Positives = 53/71 (74%)
Frame = -1 / -2
Query: 684 GALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNYLAVLIEYEDSDSAL 505
GA+AK G+ +LR AG I +Q+ RV C++PG+ VTF VE GSNP Y+A+L+EYE+ D +
Sbjct: 598 GAMAKYGRNDELRHAGIINMQFKRVPCQYPGLTVTFHVEEGSNPFYMAILVEYENGDGDV 419
Query: 504 DSVDMMQTQGG 472
+D+M+++ G
Sbjct: 418 KQLDIMESRPG 386
Score = 79.0 bits (166), Expect(2) = 3e-036
Identities = 26/57 (45%), Positives = 39/57 (68%)
Frame = -1 / -2
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
TGQWVPM++SWG++W++++ + GPF++R+T SG+ LVA IP W Y S
Sbjct: 358 TGQWVPMKESWGSIWRMDAHHPMHGPFSLRVTNESGKTLVADQVIPGDWKPNKTYSS 188
>gb|BG369116.2|BG369116 HVSMEi0021M10f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0021M10f, mRNA sequence
Length = 609
Score = 141 bits (302), Expect = 5e-033
Identities = 57/93 (61%), Positives = 65/93 (69%)
Frame = -1 / +1
Query: 741 PDCPCPDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPG 562
PD PC DE VHFD+SGTAFG +AKPGQ QL G +QIQYTRV C W G+ TF V+ G
Sbjct: 1 PDGPCLDEPVHFDLSGTAFGGMAKPGQADQLCAGGRLQIQYTRVPCNWRGMHRTFKVDAG 180
Query: 561 SNPNYLAVLIEYEDSDSALDSVDMMQTQGGTTG 463
NP YLAVLIEYED D L SV++M+ G G
Sbjct: 181 FNPYYLAVLIEYEDGDGDLSSVELMENGGNGAG 279
Score = 93.6 bits (198), Expect(2) = 1e-032
Identities = 38/62 (61%), Positives = 41/62 (66%)
Frame = +2 / -2
Query: 554 GLEPGSTEKVTSTPGHSHCTRVYWIWMAPAPRSWLSCPGFAKAPNAVPLMSKWTCSSGHG 733
GL+P ST KV P H TRVYWIW P +SW + PGFA PNAVPL SKWT SS HG
Sbjct: 188 GLKPASTLKVRCIPRQLHGTRVYWIWRRPPAQSWSAWPGFAMPPNAVPLRSKWTGSSRHG 9
Query: 734 QS 739
S
Sbjct: 8 PS 3
Score = 88.1 bits (186), Expect(2) = 7e-036
Identities = 43/83 (51%), Positives = 49/83 (59%)
Frame = -2 / +2
Query: 740 PIARVLMSRSTST*AGQRSVPWRSPGRRASCVAPAPSKSSIPGCSASGLAWT*PSRWSRA 561
P R MS STST*AG+RS WRSP R + SKSS G A+G T PS+W+ A
Sbjct: 2 PTGRAWMSPSTST*AGRRSAAWRSPARPTNFARADVSKSSTHGSRATGGGCTVPSKWTPA 181
Query: 560 PTRTTWPC*SSTRIATAPSTPWT 492
T TT PC SSTR ATA W+
Sbjct: 182 STPTTSPCSSSTRTATATCRRWS 250
Score = 82.6 bits (174), Expect(2) = 7e-036
Identities = 31/55 (56%), Positives = 38/55 (69%)
Frame = -1 / +1
Query: 489 MQTQGGTTGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAG 325
+ GG W M +SWGAVW+ NSG +L PF++RLT SSG+ LVASN IPAG
Sbjct: 250 LMENGGNGAGWTKMDRSWGAVWRYNSGPMLHAPFSVRLTSSSGKTLVASNVIPAG 414
Score = 66.1 bits (138), Expect(2) = 1e-032
Identities = 30/53 (56%), Positives = 32/53 (60%)
Frame = +2 / -2
Query: 320 FHPAGMALLATSALPELKVRRMLKGPWRTEPELIFHTAPHDCCIGTHCPVVPP 478
F PAGM LLAT LPEL+VRR KG PEL HTAPHD I +PP
Sbjct: 419 FQPAGMTLLATRVLPELEVRRTEKGACNIGPELYLHTAPHDRSILVQPAPLPP 261
>gb|CK122737.1|CK122737 BES1824105j22 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010J225 5-PRIME, mRNA sequence
Length = 776
Score = 115 bits (246), Expect(2) = 2e-035
Identities = 47/86 (54%), Positives = 61/86 (70%)
Frame = -1 / +1
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P HFD+SGTAFG LAKPG+ +LR AG I IQ+TRV CE+PG+ V F VE GSN Y
Sbjct: 379 PVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFPGLKVGFHVEEGSNAVY 558
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGT 469
+A+L+EYE+ D + VD+M+ + T
Sbjct: 559 MAILVEYENGDGDVVQVDLMEXRPRT 636
Score = 53.8 bits (111), Expect(2) = 2e-035
Identities = 19/46 (41%), Positives = 29/46 (63%)
Frame = -1 / +3
Query: 477 GGTTGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASN 340
G G+W M +SWG++W ++S + LQ P +IR+ SG+ L A N
Sbjct: 636 GAAAGRWTRMPESWGSIWGLDSXNRLQXPXSIRIRNESGKKLXARN 773
>gb|BI959138.1|BI959138 HVSMEn0018F10f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0018F10f, mRNA sequence
Length = 669
Score = 126 bits (269), Expect(2) = 3e-035
Identities = 51/86 (59%), Positives = 62/86 (72%)
Frame = -1 / +3
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P + HFD+SGTAFGA+AKPG +LR +G I IQ+ RV CE+PG+ VTF VE GSNP Y
Sbjct: 327 PVAKYHFDLSGTAFGAMAKPGLSEKLRHSGIIDIQFKRVPCEFPGLKVTFHVEQGSNPVY 506
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGT 469
AVL+EYED D L VD+M+ GT
Sbjct: 507 FAVLVEYEDGDGDLVQVDLMEANSGT 584
Score = 42.3 bits (86), Expect(2) = 3e-035
Identities = 12/24 (50%), Positives = 20/24 (83%)
Frame = -1 / +1
Query: 453 PMQQSWGAVWKINSGSVLQGPFNI 382
PM++SWG++W++ SG LQ PF++
Sbjct: 592 PMRESWGSIWRVESGHRLQAPFSM 663
>gb|CK124171.1|CK124171 BES1824107e17 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010E177 5-PRIME, mRNA sequence
Length = 751
Score = 143 bits (307), Expect = 1e-033
Identities = 55/112 (49%), Positives = 77/112 (68%)
Frame = -1 / +1
Query: 633 IQIQYTRVQCEWPGVDVTFSVEPGSNPNYLAVLIEYEDSDSALDSVDMMQTQGGTTGQWV 454
I I++TRV CE+PG+ + F VE SNP Y AVL+EYED D + VD+M+++G G+W
Sbjct: 1 IDIEFTRVPCEFPGLKIGFHVEEYSNPVYFAVLVEYEDGDGDVVQVDLMESRGPGGGKWT 180
Query: 453 PMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
M++SWG+VW+++S LQ PF+IR+ SG+ LVA+ IPA W YRS
Sbjct: 181 RMRESWGSVWRLDSNHRLQAPFSIRIRNESGKTLVANKVIPANWRPNTFYRS 336
>gb|BF253787.2|BF253787 HVSMEf0002C01f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0002C01f, mRNA sequence
Length = 823
Score = 115 bits (246), Expect(2) = 1e-033
Identities = 48/88 (54%), Positives = 61/88 (69%)
Frame = -1 / +3
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P HFD+SGTAFG LAKPG+ +LR AG I IQ+TRV CE+PG+ V F VE GSN Y
Sbjct: 30 PVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFPGLKVGFHVEEGSNAVY 209
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGTTG 463
+A+L+EYE+ D + VD+M+ G G
Sbjct: 210 MAILVEYENGDGDVVHVDLMEYGRGRHG 293
Score = 47.3 bits (97), Expect(2) = 1e-033
Identities = 16/45 (35%), Positives = 27/45 (60%)
Frame = -1 / +3
Query: 465 GQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIP 331
G+W PM +SWG++W++ +Q P++IR+ G L+ IP
Sbjct: 297 GRWTPMLKSWGSIWRLYYNHGIQTPYSIRIRCEFG*TLIMRILIP 431
>gb|BQ465138.1|BQ465138 HU02M06r HU Hordeum vulgare subsp. vulgare cDNA clone HU02M06
5-PRIME, mRNA sequence
Length = 623
Score = 117 bits (251), Expect(3) = 2e-033
Identities = 48/88 (54%), Positives = 62/88 (70%)
Frame = -1 / +2
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P HFD+SGTAFG LAKPG+ +LR AG I IQ+TRV CE+PG+ V F VE GSN Y
Sbjct: 227 PVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFPGLKVGFHVEEGSNAVY 406
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGTTG 463
+A+L+EYE+ D + VD+M++ G G
Sbjct: 407 MAILVEYENGDGDVVQVDLMESGRGRRG 490
Score = 37.3 bits (75), Expect(3) = 2e-033
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = -1 / +3
Query: 426 WKINSGSVLQGPFNIRLTFSSGRALVASN 340
W+++S LQ PF+IR+ SG+ LVA N
Sbjct: 534 WRLDSNHRLQAPFSIRIRNESGKTLVARN 620
Score = 25.4 bits (49), Expect(3) = 2e-033
Identities = 6/13 (46%), Positives = 11/13 (84%)
Frame = -1 / +2
Query: 465 GQWVPMQQSWGAV 427
G+W M++SWG++
Sbjct: 494 GRWTRMRESWGSI 532
>gb|CA019924.1|CA019924 HV13L18r HV Hordeum vulgare subsp. vulgare cDNA clone HV13L18
5-PRIME, mRNA sequence
Length = 549
Score = 124 bits (265), Expect(2) = 1e-032
Identities = 47/87 (54%), Positives = 64/87 (73%)
Frame = -1 / +3
Query: 729 CPDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPN 550
C E HFDMSGT+ GA+AKPG +LR +G ++IQY RV C++PG+++ F V+ GSNP
Sbjct: 216 CLGEAAHFDMSGTSMGAMAKPGMADKLRASGIVKIQYKRVPCKYPGMNIAFKVDQGSNPF 395
Query: 549 YLAVLIEYEDSDSALDSVDMMQTQGGT 469
YL VLIE+ED D L++VD+M+ GT
Sbjct: 396 YLEVLIEFEDDDGDLNTVDLMEANCGT 476
Score = 35.4 bits (71), Expect(2) = 1e-032
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = -1 / +3
Query: 465 GQWVPMQQSWGAVWKINSGS 406
G W PM Q+WGA++++NS +
Sbjct: 471 GTWTPMVQNWGALYRLNSNT 530
>gb|BQ459308.1|BQ459308 HA01I15r HA Hordeum vulgare subsp. vulgare cDNA clone HA01I15
5-PRIME, mRNA sequence
Length = 396
Score = 82.6 bits (174), Expect(2) = 3e-032
Identities = 30/65 (46%), Positives = 47/65 (72%)
Frame = -1 / +2
Query: 666 GQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNYLAVLIEYEDSDSALDSVDMM 487
G +LR AG I +Q+ RV C++PG+ VTF VE GSNP Y+A+L+EY++ D + +D+M
Sbjct: 2 GTRDELRHAGIINMQFKRVPCQYPGLTVTFHVEEGSNPFYMAILVEYDNGDGDVKQLDIM 181
Query: 486 QTQGG 472
+++ G
Sbjct: 182 ESRPG 196
Score = 75.8 bits (159), Expect(2) = 3e-032
Identities = 25/57 (43%), Positives = 39/57 (68%)
Frame = -1 / +2
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
TG+WVPM++SWG++W++++ ++GPF +R+T SG+ LVA IP W Y S
Sbjct: 224 TGEWVPMKESWGSIWRMDAHHPMRGPFWLRVTNESGKTLVADKVIPGDWKPNKTYSS 394
>gb|BI956772.1|BI956772 HVSMEn0005E06f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005E06f, mRNA sequence
Length = 796
Score = 118 bits (252), Expect(2) = 6e-032
Identities = 48/86 (55%), Positives = 60/86 (69%)
Frame = -1 / +1
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P + HFD+SGTAFGA+AKPG +LR +G I IQ+ RV CE+PG+ VTF VE GSNP Y
Sbjct: 310 PVAKYHFDLSGTAFGAMAKPGLSEKLRHSGIIDIQFKRVPCEFPGLKVTFHVEQGSNPVY 489
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGT 469
AVL+EY D D + VD+M+ T
Sbjct: 490 FAVLVEYXDGDGDMVQVDLMEANSCT 567
Score = 39.1 bits (79), Expect(2) = 6e-032
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = -1 / +1
Query: 459 WVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRAL 352
W PM++SWG++ +++SG + P + L GR L
Sbjct: 568 WTPMRESWGSILRLDSGHGWRPPLSFALXNEGGREL 675
>gb|AV928430.1|AV928430 AV928430 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basdg18 3', mRNA sequence
Length = 704
Score = 83.1 bits (175), Expect(2) = 8e-032
Identities = 28/57 (49%), Positives = 42/57 (73%)
Frame = -1 / -2
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
+G W+ M++SWG++W+++S LQGPF++R+T SG+ LVA+N IPA W YRS
Sbjct: 526 SGSWMAMRESWGSIWRMDSNHRLQGPFSMRITSDSGKKLVANNVIPANWRPNTDYRS 356
Score = 73.9 bits (155), Expect(2) = 8e-032
Identities = 31/62 (50%), Positives = 40/62 (64%)
Frame = -1 / -2
Query: 654 QLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNYLAVLIEYEDSDSALDSVDMMQTQG 475
+LR +G I IQ+ RV C +PG+ + F V GSN YLAVLIEYED D + VDM +
Sbjct: 703 KLRHSGIIDIQFRRVPCNFPGLKINFHVVEGSNAVYLAVLIEYEDMDGDVIQVDMKEANS 524
Query: 474 GT 469
G+
Sbjct: 523 GS 518
>gb|CV054003.1|CV054003 BNEL105f8 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL105f8 5' similar to Oryza sativa
beta-expansin, mRNA sequence
Length = 702
Score = 116 bits (248), Expect(2) = 3e-031
Identities = 44/85 (51%), Positives = 64/85 (75%)
Frame = -1 / +3
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P + HFD+SGTAFGA+AK G+ +LR AG I +Q+ RV C++PG+ VTF VE GSNP Y
Sbjct: 369 PVSRYHFDLSGTAFGAMAKYGRNDELRHAGIINMQFKRVPCQYPGLTVTFHVEEGSNPFY 548
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGG 472
+A+L+EYE+ D + +D+M+++ G
Sbjct: 549 MAILVEYENGDGDVKQLDIMESRPG 623
Score = 38.6 bits (78), Expect(2) = 3e-031
Identities = 10/17 (58%), Positives = 16/17 (94%)
Frame = -1 / +3
Query: 468 TGQWVPMQQSWGAVWKI 418
TGQWVPM++SW ++W++
Sbjct: 651 TGQWVPMKESWXSIWRM 701
>gb|BQ470849.1|BQ470849 HX04D14r HX Hordeum vulgare subsp. vulgare cDNA clone HX04D14
5-PRIME, mRNA sequence
Length = 606
Score = 80.8 bits (170), Expect(2) = 5e-031
Identities = 27/57 (47%), Positives = 40/57 (70%)
Frame = -1 / +2
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
TG+WVPM +SWG++W++++ +QGPF++R+T SG+ LVA IPA W Y S
Sbjct: 179 TGEWVPMTESWGSIWRMDTRRPMQGPFSLRITNESGKTLVADQVIPADWEPNEIYSS 349
Score = 73.5 bits (154), Expect(2) = 5e-031
Identities = 27/46 (58%), Positives = 37/46 (80%)
Frame = -1 / +2
Query: 615 RVQCEWPGVDVTFSVEPGSNPNYLAVLIEYEDSDSALDSVDMMQTQ 478
RV C++PG+ VTF V+ GSNP YLA+L+EYE+ D +D VDMMQ++
Sbjct: 5 RVPCQYPGLTVTFHVQHGSNPYYLAILVEYENGDGDVDQVDMMQSR 142
>gb|CV063217.1|CV063217 BNEL87h3 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL87h3 5' similar to Oryza sativa
beta-expansin, mRNA sequence
Length = 701
Score = 113 bits (241), Expect(2) = 7e-031
Identities = 43/85 (50%), Positives = 63/85 (74%)
Frame = -1 / +3
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P + HFD+SGTAFGA+AK G+ +LR AG I +Q+ RV C++P + VTF VE GSNP Y
Sbjct: 375 PVSRYHFDLSGTAFGAMAKYGRNDELRHAGIINMQFKRVPCQYPXLTVTFHVEEGSNPFY 554
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGG 472
+A+L+EYE+ D + +D+M+++ G
Sbjct: 555 MAILVEYENGDGDVKQLDIMESRPG 629
Score = 40.5 bits (82), Expect(2) = 7e-031
Identities = 11/15 (73%), Positives = 15/15 (100%)
Frame = -1 / +3
Query: 468 TGQWVPMQQSWGAVW 424
TGQWVPM++SWG++W
Sbjct: 657 TGQWVPMKESWGSIW 701
>gb|CV058907.1|CV058907 BNEL42c7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL42c7 5' similar to beta-expansin,
mRNA sequence
Length = 502
Score = 133 bits (284), Expect = 1e-030
Identities = 52/84 (61%), Positives = 60/84 (71%)
Frame = -1 / +3
Query: 492 MMQTQGGTTGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAG 313
+ + G TG W+PMQQSWGAVWK+NSGS LQGPF+IRLTF SG+ LVA NAIP GWN G
Sbjct: 21 LRSSTAGATGVWIPMQQSWGAVWKLNSGSTLQGPFSIRLTFRSGKMLVAGNAIPTGWNPG 200
Query: 312 VAYRSGGVAVTRARPRSGACRGYE 241
VAY+ GGVAV G+E
Sbjct: 201 VAYQPGGVAVRERASGGRRQHGFE 272
Score = 127 bits (273), Expect = 5e-029
Identities = 49/65 (75%), Positives = 51/65 (78%)
Frame = +2 / -1
Query: 281 VTATPPDRYATPAFHPAGMALLATSALPELKVRRMLKGPWRTEPELIFHTAPHDCCIGTH 460
+TATPP YATP FHP GMAL ATS LP+L V RM KGPWR EPEL FHTAPHDCCIG H
Sbjct: 232 LTATPPGWYATPGFHPVGMALPATSILPDLNVSRMEKGPWRVEPELSFHTAPHDCCIGIH 53
Query: 461 CPVVP 475
PV P
Sbjct: 52 TPVAP 38
>gb|CB881182.1|CB881182 HM09A07w HM Hordeum vulgare subsp. vulgare cDNA clone HM09A07
3-PRIME, mRNA sequence
Length = 680
Score = 67.0 bits (140), Expect(3) = 2e-030
Identities = 22/55 (40%), Positives = 35/55 (63%)
Frame = -1 / -3
Query: 462 QWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
+W+ +++SWGAVW+I++ L GPF++R T G VA + IP GW +Y +
Sbjct: 423 KWIELKESWGAVWRIDTPDKLIGPFSVRYTTEGGTKTVAEDVIPEGWKPDTSYET 259
Score = 62.9 bits (131), Expect(3) = 2e-030
Identities = 22/39 (56%), Positives = 32/39 (82%)
Frame = -1 / -3
Query: 711 HFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWP 595
HFD+SG AFG +AK GQE +LR AG ++I++ RV+C++P
Sbjct: 666 HFDLSGHAFGTMAKKGQEQKLRDAGEVEIKFRRVKCKYP 550
Score = 40.0 bits (81), Expect(3) = 2e-030
Identities = 15/35 (42%), Positives = 24/35 (68%)
Frame = -1 / -3
Query: 594 GVDVTFSVEPGSNPNYLAVLIEYEDSDSALDSVDM 490
G V F VE GS+PNYLA+++++ D + +VD+
Sbjct: 546 GTKVNFHVEKGSSPNYLALVVKFLQGDGDVVAVDI 442
>gb|CA017263.1|CA017263 HV14A15u HV Hordeum vulgare subsp. vulgare cDNA clone HV14A15
3-PRIME, mRNA sequence
Length = 569
Score = 75.3 bits (158), Expect(2) = 7e-030
Identities = 26/56 (46%), Positives = 37/56 (66%)
Frame = -1 / -2
Query: 465 GQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
G+W M++SWG++W+++S LQ PF+IR+ SG+ LVA N IP W YRS
Sbjct: 388 GRWTRMRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIPKNWRPNTFYRS 221
Score = 75.3 bits (158), Expect(2) = 7e-030
Identities = 30/59 (50%), Positives = 41/59 (69%)
Frame = -1 / -2
Query: 639 GAIQIQYTRVQCEWPGVDVTFSVEPGSNPNYLAVLIEYEDSDSALDSVDMMQTQGGTTG 463
G I IQ+TRV CE+PG+ V F VE GSN Y+A+L+EYE+ D + VD+M++ G G
Sbjct: 568 GIIDIQFTRVACEFPGLKVGFHVEEGSNAVYMAILVEYENGDGDVVQVDLMESGRGRRG 392
>gb|CA019997.1|CA019997 HV14A15r HV Hordeum vulgare subsp. vulgare cDNA clone HV14A15
5-PRIME, mRNA sequence
Length = 616
Score = 117 bits (251), Expect(2) = 1e-027
Identities = 48/88 (54%), Positives = 62/88 (70%)
Frame = -1 / +1
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P HFD+SGTAFG LAKPG+ +LR AG I IQ+TRV CE+PG+ V F VE GSN Y
Sbjct: 310 PVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFPGLKVGFHVEEGSNAVY 489
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGTTG 463
+A+L+EYE+ D + VD+M++ G G
Sbjct: 490 MAILVEYENGDGDVVQVDLMESGRGRRG 573
Score = 25.4 bits (49), Expect(2) = 1e-027
Identities = 6/13 (46%), Positives = 11/13 (84%)
Frame = -1 / +1
Query: 465 GQWVPMQQSWGAV 427
G+W M++SWG++
Sbjct: 577 GRWTRMRESWGSI 615
>gb|BE193443.3|BE193443 HVSMEh0081A10f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0081A10f, mRNA sequence
Length = 749
Score = 105 bits (223), Expect(2) = 1e-027
Identities = 40/64 (62%), Positives = 45/64 (70%)
Frame = -1 / +3
Query: 744 CPDCPCPDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEP 565
CPD PC DE VHFD+SGTAFG +AKPGQ QLR AG +QIQYT V C W G+ V V+
Sbjct: 390 CPDGPCLDEPVHFDLSGTAFGGMAKPGQADQLRAAGRLQIQYTPVPCNWRGMHVALQVDA 569
Query: 564 GSNP 553
G P
Sbjct: 570 GLQP 581
Score = 37.7 bits (76), Expect(2) = 1e-027
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = -1 / +1
Query: 579 FSVEPGSNPNYLAVLIEYEDSDSALDSVDMMQTQG 475
F PG NP YLA I+YED D S ++M+ G
Sbjct: 556 FRWTPGFNPYYLAGAIQYEDGDGEPSSGELMENGG 660
>gb|AV917082.1|AV917082 AV917082 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20l09 5', mRNA sequence
Length = 323
Score = 117 bits (251), Expect(2) = 3e-027
Identities = 48/88 (54%), Positives = 62/88 (70%)
Frame = -1 / +2
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P HFD+SGTAFG LAKPG+ +LR AG I IQ+TRV CE+PG+ V F VE GSN Y
Sbjct: 20 PVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFPGLKVGFHVEEGSNAVY 199
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGGTTG 463
+A+L+EYE+ D + VD+M++ G G
Sbjct: 200 MAILVEYENGDGDVVQVDLMESGRGRRG 283
Score = 23.5 bits (45), Expect(2) = 3e-027
Identities = 6/11 (54%), Positives = 9/11 (81%)
Frame = -1 / +2
Query: 465 GQWVPMQQSWG 433
G+W M++SWG
Sbjct: 287 GRWTRMRESWG 319
>gb|BU967134.1|BU967134 HB03G05r BC Hordeum vulgare subsp. vulgare cDNA clone HB03G05
5-PRIME, mRNA sequence
Length = 430
Score = 116 bits (248), Expect(2) = 8e-026
Identities = 44/85 (51%), Positives = 64/85 (75%)
Frame = -1 / +1
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P + HFD+SGTAFGA+AK G+ +LR AG I +Q+ RV C++PG+ VTF VE GSNP Y
Sbjct: 124 PVSRYHFDLSGTAFGAMAKYGRNDELRHAGIINMQFKRVPCQYPGLTVTFHVEEGSNPFY 303
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGG 472
+A+L+EYE+ D + +D+M+++ G
Sbjct: 304 MAILVEYENGDGDVKQLDIMESRPG 378
Score = 21.7 bits (41), Expect(2) = 8e-026
Identities = 6/8 (75%), Positives = 8/8 (100%)
Frame = -1 / +1
Query: 468 TGQWVPMQ 445
TG+WVPM+
Sbjct: 406 TGEWVPMK 429
>gb|BM101447.2|BM101447 EBpi01_SQ003_O01_R pistil, 1 DPA, no treatment, cv Optic, EBpi01
Hordeum vulgare subsp. vulgare cDNA clone
EBpi01_SQ003_O01 5', mRNA sequence
Length = 452
Score = 75.3 bits (158), Expect(2) = 1e-025
Identities = 26/56 (46%), Positives = 37/56 (66%)
Frame = -1 / +1
Query: 465 GQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
G+W M++SWG++W+++S LQ PF+IR+ SG+ LVA N IP W YRS
Sbjct: 154 GRWTRMRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIPKNWRPNTFYRS 321
Score = 61.1 bits (127), Expect(2) = 1e-025
Identities = 24/50 (48%), Positives = 34/50 (68%)
Frame = -1 / +1
Query: 612 VQCEWPGVDVTFSVEPGSNPNYLAVLIEYEDSDSALDSVDMMQTQGGTTG 463
V CE+PG+ V F VE GSN Y+A+L+EYE+ D + VD+M++ G G
Sbjct: 1 VACEFPGLKVGFHVEEGSNAVYMAILVEYENGDGDVVQVDLMESGRGRRG 150
>gb|BU981055.1|BU981055 HA22I03r HA Hordeum vulgare subsp. vulgare cDNA clone HA22I03
5-PRIME, mRNA sequence
Length = 597
Score = 116 bits (248), Expect = 1e-025
Identities = 44/85 (51%), Positives = 64/85 (75%)
Frame = -1 / +1
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P + HFD+SGTAFGA+AK G+ +LR AG I +Q+ RV C++PG+ VTF VE GSNP Y
Sbjct: 322 PVSRYHFDLSGTAFGAMAKYGRNDELRHAGIINMQFKRVPCQYPGLTVTFHVEEGSNPFY 501
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGG 472
+A+L+EYE+ D + +D+M+++ G
Sbjct: 502 MAILVEYENGDGDVKQLDIMESRPG 576
>gb|CV063939.1|CV063939 BNEL94h5 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL94h5 5' similar to Oryza sativa
beta-expansin, mRNA sequence
Length = 661
Score = 116 bits (248), Expect = 1e-025
Identities = 44/85 (51%), Positives = 64/85 (75%)
Frame = -1 / +2
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P + HFD+SGTAFGA+AK G+ +LR AG I +Q+ RV C++PG+ VTF VE GSNP Y
Sbjct: 368 PVSRYHFDLSGTAFGAMAKYGRNDELRHAGIINMQFKRVPCQYPGLTVTFHVEEGSNPFY 547
Query: 546 LAVLIEYEDSDSALDSVDMMQTQGG 472
+A+L+EYE+ D + +D+M+++ G
Sbjct: 548 MAILVEYENGDGDVKQLDIMESRPG 622
>gb|BE196421.3|BE196421 HVSMEh0092D24f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0092D24f, mRNA sequence
Length = 901
Score = 67.5 bits (141), Expect(3) = 2e-025
Identities = 26/44 (59%), Positives = 33/44 (75%)
Frame = -1 / +1
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWP 595
P + HFD+SGTAFGA+AKPG +LR +G I IQ+ RV CE+P
Sbjct: 349 PVAKYHFDLSGTAFGAMAKPGLSEKLRHSGIIDIQFKRVPCEFP 480
Score = 46.4 bits (95), Expect(3) = 2e-025
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = -1 / +3
Query: 477 GGTTGQWVPMQQSWGAVWKINSGSVLQGPFN 385
G G W PM++SWG++W ++ G LQ PF+
Sbjct: 588 GANFGTWTPMRESWGSIWGLDFGHRLQAPFS 680
Score = 39.6 bits (80), Expect(3) = 2e-025
Identities = 18/28 (64%), Positives = 18/28 (64%)
Frame = -1 / +3
Query: 597 PGVDVTFSVEPGSNPNYLAVLIEYEDSD 514
P VTF VE SNP Y AVL EYED D
Sbjct: 477 PLSQVTFHVEQXSNPVYFAVLGEYEDGD 560
>gb|BI957603.1|BI957603 HVSMEn0010F11f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0010F11f, mRNA sequence
Length = 630
Score = 114 bits (244), Expect = 5e-025
Identities = 47/81 (58%), Positives = 58/81 (71%)
Frame = -1 / +1
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P + HFD+SGTAFGA+AKPG +LR +G I IQ+ RV CE+PG+ VTF VE GSNP Y
Sbjct: 385 PVAKYHFDLSGTAFGAMAKPGLSEKLRHSGIIDIQFKRVPCEFPGLKVTFHVEQGSNPVY 564
Query: 546 LAVLIEYEDSDSALDSVDMMQ 484
VL+EY D D L VD+M+
Sbjct: 565 FPVLVEY*DGDGNLVQVDLME 627
>gb|BI958784.1|BI958784 HVSMEn0016J13f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0016J13f, mRNA sequence
Length = 656
Score = 114 bits (244), Expect = 5e-025
Identities = 47/78 (60%), Positives = 56/78 (71%)
Frame = -1 / +3
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P + HFD+SGTAFGA+AKPG +LR +G I IQ+ RV CE+PG+ VTF VE GSNP Y
Sbjct: 423 PVAKYHFDLSGTAFGAMAKPGLSEKLRHSGIIDIQFKRVPCEFPGLKVTFHVEQGSNPVY 602
Query: 546 LAVLIEYEDSDSALDSVD 493
AVL+EYED D VD
Sbjct: 603 FAVLVEYEDGDGDXVXVD 656
>gb|BQ458429.1|BQ458429 HA05E07r HA Hordeum vulgare subsp. vulgare cDNA clone HA05E07
5-PRIME, mRNA sequence
Length = 504
Score = 113 bits (242), Expect = 9e-025
Identities = 43/81 (53%), Positives = 61/81 (75%)
Frame = -1 / +2
Query: 726 PDEQVHFDMSGTAFGALAKPGQESQLRGAGAIQIQYTRVQCEWPGVDVTFSVEPGSNPNY 547
P + HFD+SGTAFGA+AK G+ +LR AG I +Q+ RV C++PG+ VTF VE GSNP Y
Sbjct: 260 PVSRYHFDLSGTAFGAMAKYGRNDELRHAGIINMQFKRVPCQYPGLTVTFHVEEGSNPFY 439
Query: 546 LAVLIEYEDSDSALDSVDMMQ 484
+A+L+EYE+ D + +D+M+
Sbjct: 440 MAILVEYENGDGDVKQLDIME 502
>gb|CB880652.1|CB880652 HM07E17w HM Hordeum vulgare subsp. vulgare cDNA clone HM07E17
3-PRIME, mRNA sequence
Length = 562
Score = 82.2 bits (173), Expect(2) = 1e-024
Identities = 28/57 (49%), Positives = 40/57 (70%)
Frame = -1 / -2
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
+G W PM++SWG++W+++SG LQ PF++R+T SG+ LVA IPA W YRS
Sbjct: 462 SGTWTPMRESWGSIWRLDSGHRLQAPFSMRITNESGKTLVADKVIPANWAPSTFYRS 292
Score = 51.0 bits (105), Expect(2) = 1e-024
Identities = 20/35 (57%), Positives = 24/35 (68%)
Frame = -1 / -2
Query: 573 VEPGSNPNYLAVLIEYEDSDSALDSVDMMQTQGGT 469
VE GSNP Y AVL+EYED D + VD+M+ GT
Sbjct: 558 VEQGSNPVYFAVLVEYEDGDGDVVQVDLMEANSGT 454
>gb|CB881234.1|CB881234 HM09C23w HM Hordeum vulgare subsp. vulgare cDNA clone HM09C23
3-PRIME, mRNA sequence
Length = 563
Score = 82.2 bits (173), Expect(2) = 1e-024
Identities = 28/57 (49%), Positives = 40/57 (70%)
Frame = -1 / -2
Query: 468 TGQWVPMQQSWGAVWKINSGSVLQGPFNIRLTFSSGRALVASNAIPAGWNAGVAYRS 298
+G W PM++SWG++W+++SG LQ PF++R+T SG+ LVA IPA W YRS
Sbjct: 463 SGTWTPMRESWGSIWRLDSGHRLQAPFSMRITNESGKTLVADKVIPANWAPSTFYRS 293
Score = 51.0 bits (105), Expect(2) = 1e-024
Identities = 20/35 (57%), Positives = 24/35 (68%)
Frame = -1 / -2
Query: 573 VEPGSNPNYLAVLIEYEDSDSALDSVDMMQTQGGT 469
VE GSNP Y AVL+EYED D + VD+M+ GT
Sbjct: 559 VEQGSNPVYFAVLVEYEDGDGDVVQVDLMEANSGT 455
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 392,373,797
Number of Sequences: 312970
Number of extensions: 7241697
Number of successful extensions: 140269
Number of sequences better than 1.0e-020: 88
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8313674964
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)