TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2591248.2.1
(1222 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI779097.2|BI779097 EBro01_SQ002_P16_R root, 3 week, hyd... 243 2e-063
gb|BU985192.1|BU985192 HF06G12r HF Hordeum vulgare subsp. v... 190 1e-060
gb|BQ470997.1|BQ470997 HX04K17r HX Hordeum vulgare subsp. v... 97 5e-053
gb|BJ467008.1|BJ467008 BJ467008 K. Sato unpublished cDNA li... 156 2e-050
gb|CA023829.1|CA023829 HZ47I13r HZ Hordeum vulgare subsp. v... 184 4e-048
gb|CK123410.1|CK123410 BES1824103p13 BES1824 Hordeum vulgar... 149 1e-045
gb|BM099274.2|BM099274 EBes01_SQ002_M04_R embryo sac, 4-6 D... 138 1e-040
gb|BQ664895.1|BQ664895 HX01E21w HX Hordeum vulgare subsp. v... 164 1e-039
gb|BF256953.2|BF256953 HVSMEf0011H11f Hordeum vulgare seedl... 154 9e-037
gb|CK125884.1|CK125884 BES1824111A2a04 BES1824 Hordeum vulg... 125 2e-034
gb|BF259829.2|BF259829 HVSMEf0020G01f Hordeum vulgare seedl... 124 2e-033
gb|AJ461943.1|AJ461943 AJ461943 S00002 Hordeum vulgare subs... 132 5e-030
gb|CA027804.1|CA027804 HZ60B10r HZ Hordeum vulgare subsp. v... 94 8e-029
gb|CK122715.1|CK122715 BES1824105i22 BES1824 Hordeum vulgar... 125 4e-028
gb|CK123273.1|CK123273 BES1824103c09 BES1824 Hordeum vulgar... 125 4e-028
gb|BG369061.1|BG369061 HVSMEi0021J08f Hordeum vulgare 20 DA... 81 4e-027
gb|BU995458.1|BU995458 HM10F02r HM Hordeum vulgare subsp. v... 81 4e-027
gb|BJ464625.1|BJ464625 BJ464625 K. Sato unpublished cDNA li... 85 7e-027
gb|BQ766009.1|BQ766009 EBro03_SQ008_D24_R root, 3 week, wat... 93 4e-026
gb|BM377965.2|BM377965 EBem04_SQ004_L21_R embryo, 12 DPA, n... 116 3e-025
gb|BI956376.1|BI956376 HVSMEn0002P20f Hordeum vulgare rachi... 115 6e-025
gb|BI960651.1|BI960651 HVSMEn0025K02f Hordeum vulgare rachi... 88 1e-024
gb|BQ460303.1|BQ460303 HA06K13r HA Hordeum vulgare subsp. v... 112 3e-024
gb|CK125396.1|CK125396 BES1824107o06 BES1824 Hordeum vulgar... 86 1e-022
gb|CB858324.1|CB858324 HI06J16w HI Hordeum vulgare subsp. v... 106 2e-022
gb|BU996208.1|BU996208 HM12N12r HM Hordeum vulgare subsp. v... 64 5e-022
>gb|BI779097.2|BI779097 EBro01_SQ002_P16_R root, 3 week, hydroponic grown, no treatment, cv
Optic, EBro01 Hordeum vulgare subsp. vulgare cDNA clone
EBro01_SQ002_P16 5', mRNA sequence
Length = 351
Score = 243 bits (525), Expect = 2e-063
Identities = 100/116 (86%), Positives = 108/116 (93%)
Frame = -1 / +2
Query: 916 NVLISKEVLMGKDEYFQSFRVNADNFMCTLLPGISNHPQIQYSPGGLLFKVGSSNMQHVT 737
N+LISKEVLMGKD +F+SF+VNADNF+CTLLPGISNHPQI+YSPGGLLFKVG+SNMQHVT
Sbjct: 2 NILISKEVLMGKDSFFKSFQVNADNFICTLLPGISNHPQIEYSPGGLLFKVGNSNMQHVT 181
Query: 736 QLSFLLLAYSNYLSHAGGRVSCGSSSASPVQLRRVAKRQVDYILGDNPLRMSYMVG 569
LSFLLLAY+NY SHA RV CG SSASPV LRRVAKRQVDYILGDNPLRMSYMVG
Sbjct: 182 SLSFLLLAYANYASHANVRVPCGGSSASPVVLRRVAKRQVDYILGDNPLRMSYMVG 349
Score = 199 bits (428), Expect = 4e-050
Identities = 88/117 (75%), Positives = 93/117 (79%)
Frame = +3 / -1
Query: 567 YPTMYDIRSGLSPSM*STCRLATRRSCTGDADDEPHETRPPAWLR*LE*ASRRNDSCVTC 746
YPTMYD+RSGLSP M*ST RLATRRS TG+A++ PH TR AWL *L *ASRRNDS VTC
Sbjct: 351 YPTMYDMRSGLSPRM*STWRLATRRSTTGEAEEPPHGTRTLAWLA*LA*ASRRNDSDVTC 172
Query: 747 CMLLLPTLNSSPPGEYWIWG*LEMPGRRVHMKLSALTRKDWKYSSFPIRTSFEMRTL 917
CMLLLPTLNS PPGEY IWG LE+PGRRV MKLSA T D K S PI TS EMR L
Sbjct: 171 CMLLLPTLNSRPPGEYSIWGWLEIPGRRVQMKLSAFTWNDLKKESLPISTSLEMRML 1
Score = 121 bits (258), Expect(2) = 3e-032
Identities = 49/71 (69%), Positives = 57/71 (80%)
Frame = -3 / +1
Query: 917 QRPHLKGGSDGEGRVLPVLPCQRRQLHVHPPPRHLQSPPDPVLPRRAAVQGREQQHAARD 738
Q PHL+GG+DG+G +L V+P +RRQLH+HPPP LQ PPD VLPR AVQG +QQHAARD
Sbjct: 1 QHPHLQGGADGQGLLLQVVPGERRQLHLHPPPGDLQPPPDRVLPRWPAVQGGQQQHAARD 180
Query: 737 AAVVPPAGLLQ 705
AVVPPA L Q
Sbjct: 181 VAVVPPARLRQ 213
Score = 103 bits (220), Expect(2) = 2e-026
Identities = 47/65 (72%), Positives = 49/65 (75%)
Frame = +2 / -3
Query: 722 EERQLRHVLHVAAPDLEQQPAWGVLDLGVIGDAGEEGAHEVVGVDTEGLEVLVLPHQNLL 901
EERQ RHVLHVA LEQQ WGVLDLGV+GD EEGA EVVGV E LE VL HQ+LL
Sbjct: 196 EERQRRHVLHVAVAHLEQQATWGVLDLGVVGDPREEGADEVVGVHLERLEEGVLAHQHLL 17
Query: 902 *DEDV 916
DEDV
Sbjct: 16 GDEDV 2
Score = 38.2 bits (77), Expect(2) = 3e-032
Identities = 12/14 (85%), Positives = 12/14 (85%)
Frame = -2 / +3
Query: 621 RWTTYWATTRCGCR 580
RWTT WATTRC CR
Sbjct: 297 RWTTSWATTRCACR 338
Score = 36.3 bits (73), Expect(2) = 2e-026
Identities = 12/14 (85%), Positives = 12/14 (85%)
Frame = +1 / -2
Query: 571 PPCTTSAAGCRPVC 612
PPCTT AAGCRP C
Sbjct: 347 PPCTTCAAGCRPGC 306
>gb|BU985192.1|BU985192 HF06G12r HF Hordeum vulgare subsp. vulgare cDNA clone HF06G12
5-PRIME, mRNA sequence
Length = 615
Score = 190 bits (409), Expect(2) = 1e-060
Identities = 73/118 (61%), Positives = 90/118 (76%)
Frame = -1 / +3
Query: 754 NMQHVTQLSFLLLAYSNYLSHAGGRVSCGSSSASPVQLRRVAKRQVDYILGDNPLRMSYM 575
NMQHVT +SFLLL Y+ YL+ + V+CG S P LR AK+Q+DY+LGDNP++MSYM
Sbjct: 3 NMQHVTSISFLLLTYAKYLTKSSHTVNCGDISVGPDTLRLQAKKQIDYLLGDNPMKMSYM 182
Query: 574 VGYGARFPRRIHHRASSLPSVAAHPARIGCKAGAAYYTSPAPNPNLLVGAVVGGPTDD 401
VGYG R+P+RIHHR SSLPS+ HP R+ CK G YY S +PNPN LVGA+VGGP +D
Sbjct: 183 VGYGDRYPQRIHHRGSSLPSIKDHPERMACKDGTPYYDSSSPNPNPLVGAIVGGPGED 356
Score = 88.1 bits (186), Expect(3) = 2e-024
Identities = 41/88 (46%), Positives = 53/88 (60%)
Frame = +3 / -3
Query: 492 IRAGCAATDGSELARWWIRRGNRAPYPTMYDIRSGLSPSM*STCRLATRRSCTGDADDEP 671
+R+G + +G + RWWIR G +PYPTMYDI GLSP+ *S C LA R +G + P
Sbjct: 265 MRSGWSLIEGKDDPRWWIR*GYLSPYPTMYDIFIGLSPNR*SICFLACSRRVSGPTEMSP 86
Query: 672 HETRPPAWLR*LE*ASRRNDSCVTCCML 755
T ++R L * RN+ VTCCML
Sbjct: 85 QLTV*EDFVRYLA*VRSRNEMEVTCCML 2
Score = 63.8 bits (133), Expect(2) = 1e-060
Identities = 24/42 (57%), Positives = 33/42 (78%)
Frame = -1 / +3
Query: 415 GPTDDSDAFPDARAVFQQSEPTTYINAPLMGLLAYFSAHPNP 290
G + D++ D RA +++SEPTTYINAPL+G+LAYF +PNP
Sbjct: 339 GGPGEDDSYGDDRADYRKSEPTTYINAPLVGVLAYFVGNPNP 464
Score = 37.7 bits (76), Expect(3) = 2e-024
Identities = 22/42 (52%), Positives = 24/42 (57%)
Frame = +3 / -3
Query: 291 GLGCAEK*ARRPMSGALMYVVGSDCWNTARASGKASESSVGP 416
G G K*AR P GALM VVGSD +AR+S S S P
Sbjct: 463 GFGLPTK*ARTPTRGALM*VVGSDFL*SARSSP*ESSSPGPP 338
Score = 24.4 bits (47), Expect(3) = 2e-024
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +3 / -3
Query: 402 SSVGPPTTAPTS 437
SS GPPT APT+
Sbjct: 355 SSPGPPTMAPTN 320
>gb|BQ470997.1|BQ470997 HX04K17r HX Hordeum vulgare subsp. vulgare cDNA clone HX04K17
5-PRIME, mRNA sequence
Length = 627
Score = 97.3 bits (206), Expect(3) = 5e-053
Identities = 39/56 (69%), Positives = 45/56 (80%)
Frame = -2 / +1
Query: 1218 SIVFRDADPAYSKRLLDRAVAVFEFADKYRGPYSSSLHAAVCPCYCDYSGYQDELL 1051
S+VFR +DPAYS RL+ RA VFEFADK+RG YS+ L + VCP YC YSGYQDELL
Sbjct: 106 SLVFRKSDPAYSSRLVARAKRVFEFADKHRGVYSAKLSSYVCPYYCSYSGYQDELL 273
Score = 96.4 bits (204), Expect(3) = 5e-053
Identities = 36/74 (48%), Positives = 51/74 (68%)
Frame = -1 / +1
Query: 1039 AWLHKATRRREYREYIKRNEVVLGASDAINEFGWDNKHAGINVLISKEVLMGKDEYFQSF 860
AWLH+AT+ Y YIK N +LGA + N FGWDNKHAG +L+SK L+ K Q +
Sbjct: 283 AWLHRATKSPVYLSYIKLNGQLLGADEQDNTFGWDNKHAGARILLSKSFLVQKVGALQEY 462
Query: 859 RVNADNFMCTLLPG 818
+ +AD+F+C+++PG
Sbjct: 463 KAHADSFICSMVPG 504
Score = 62.9 bits (131), Expect(3) = 1e-024
Identities = 34/81 (41%), Positives = 42/81 (51%)
Frame = +3 / -2
Query: 816 MPGRRVHMKLSALTRKDWKYSSFPIRTSFEMRTLMPACLLSQPNSLMASLAPSTTSFLLM 995
+PG MKLSA +F R E R L PACLLS P L S APS+ F LM
Sbjct: 506 VPGTMEQMKLSACALYSCSAPTFCTRKDLESRILAPACLLSHPKVLSCSSAPSSWPFSLM 327
Query: 996 YSRYSRRRVALCSHAKKNTAA 1058
R + VA CSHA ++++
Sbjct: 326 *LR*TGLLVARCSHAAPHSSS 264
Score = 58.3 bits (121), Expect(3) = 1e-024
Identities = 31/57 (54%), Positives = 33/57 (57%)
Frame = +2 / -2
Query: 1049 HXXXXWYPE*SQ*QGHTAAWRLLL*GPRYLSANSNTATARSRRRLE*AGSASRKTME 1219
H WYPE* Q* G T L L P LSANSNT A + RLE*AGS RKT +
Sbjct: 275 HSSSSWYPE*EQ**GQTYDESLALYTPLCLSANSNTLLALATSRLE*AGSDLRKTRD 105
Score = 53.8 bits (111), Expect(3) = 5e-053
Identities = 22/35 (62%), Positives = 26/35 (74%)
Frame = -1 / +1
Query: 802 QIQYSPGGLLFKVGSSNMQHVTQLSFLLLAYSNYL 698
Q Y+ GGLLFK+ SNMQ+VT SFLLL Y+ YL
Sbjct: 517 QTTYTRGGLLFKLSDSNMQYVTSSSFLLLTYAKYL 621
Score = 29.9 bits (59), Expect(3) = 1e-024
Identities = 16/35 (45%), Positives = 19/35 (54%)
Frame = +3 / -2
Query: 699 R*LE*ASRRNDSCVTCCMLLLPTLNSSPPGEYWIW 803
R L * SRR + VT CMLL +L PP +W
Sbjct: 620 RYLA*VSRRKELEVTYCMLLSLSLKRRPPRV*VVW 516
>gb|BJ467008.1|BJ467008 BJ467008 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags35i04 3', mRNA sequence
Length = 786
Score = 156 bits (335), Expect(2) = 2e-050
Identities = 59/93 (63%), Positives = 72/93 (77%)
Frame = -1 / -3
Query: 679 VSCGSSSASPVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHP 500
V+CG S P LR AK+Q+DY+LGDNP++MSYMVGYG R+P+RIHHR SSLPS+ HP
Sbjct: 778 VNCGDISVGPDTLRLQAKKQIDYLLGDNPMKMSYMVGYGDRYPQRIHHRGSSLPSIKDHP 599
Query: 499 ARIGCKAGAAYYTSPAPNPNLLVGAVVGGPTDD 401
R+ CK G YY S +PNPN LVGA+VGGP +D
Sbjct: 598 ERMACKDGTPYYDSSSPNPNPLVGAIVGGPGED 500
Score = 63.8 bits (133), Expect(2) = 2e-050
Identities = 24/42 (57%), Positives = 33/42 (78%)
Frame = -1 / -3
Query: 415 GPTDDSDAFPDARAVFQQSEPTTYINAPLMGLLAYFSAHPNP 290
G + D++ D RA +++SEPTTYINAPL+G+LAYF +PNP
Sbjct: 517 GGPGEDDSYGDDRADYRKSEPTTYINAPLVGVLAYFVGNPNP 392
>gb|CA023829.1|CA023829 HZ47I13r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ47I13
5-PRIME, mRNA sequence
Length = 460
Score = 184 bits (396), Expect(2) = 4e-048
Identities = 75/125 (60%), Positives = 93/125 (74%)
Frame = -1 / +2
Query: 802 QIQYSPGGLLFKVGSSNMQHVTQLSFLLLAYSNYLSHAGGRVSCGSSSASPVQLRRVAKR 623
Q Y+ GGLLFK+ SNMQ+VT SFLLL Y+ YL A VSCG + +P +LR +A+R
Sbjct: 86 QTTYTRGGLLFKLSDSNMQYVTSSSFLLLTYAKYLVSAKKTVSCGGVAVTPQRLRAIARR 265
Query: 622 QVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHPARIGCKAGAAYYTSPAPNP 443
QVDY+LG NP+ MSYMVGYGA++P+++HHRASSLPSVAAHP +IGC G S A NP
Sbjct: 266 QVDYLLGSNPMGMSYMVGYGAKYPKKLHHRASSLPSVAAHPGKIGCSQGFTGLYSGAANP 445
Query: 442 NLLVG 428
N+ VG
Sbjct: 446 NVHVG 460
Score = 28.1 bits (55), Expect(2) = 4e-048
Identities = 7/17 (41%), Positives = 15/17 (88%)
Frame = -1 / +2
Query: 868 QSFRVNADNFMCTLLPG 818
Q ++ +AD+F+C+++PG
Sbjct: 23 QEYKAHADSFICSMVPG 73
>gb|CK123410.1|CK123410 BES1824103p13 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P133 5-PRIME, mRNA sequence
Length = 579
Score = 149 bits (321), Expect(2) = 1e-045
Identities = 58/93 (62%), Positives = 73/93 (78%)
Frame = -1 / +1
Query: 679 VSCGSSSASPVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHP 500
VSCG + +P +LR +A+RQVDY+LG NP+ MSYMVGYGA++P+++HHRASSLPSVAAHP
Sbjct: 22 VSCGGVAVTPQRLRAIARRQVDYLLGSNPMGMSYMVGYGAKYPKKLHHRASSLPSVAAHP 201
Query: 499 ARIGCKAGAAYYTSPAPNPNLLVGAVVGGPTDD 401
+IGC G S A NPN+ VGAVVGGP +
Sbjct: 202 GKIGCSQGFTGLYSGAANPNVHVGAVVGGPNQN 300
Score = 54.2 bits (112), Expect(2) = 1e-045
Identities = 21/39 (53%), Positives = 28/39 (71%)
Frame = -1 / +1
Query: 421 VGGPTDDSDAFPDARAVFQQSEPTTYINAPLMGLLAYFS 305
V G + +D FPD R+ ++ SEP TYINAPL+G LAY +
Sbjct: 277 VVGGPNQNDQFPDQRSDYEHSEPATYINAPLVGALAYLA 393
>gb|BM099274.2|BM099274 EBes01_SQ002_M04_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ002_M04 5', mRNA sequence
Length = 464
Score = 138 bits (297), Expect(2) = 1e-040
Identities = 54/91 (59%), Positives = 70/91 (76%)
Frame = -1 / +3
Query: 802 QIQYSPGGLLFKVGSSNMQHVTQLSFLLLAYSNYLSHAGGRVSCGSSSASPVQLRRVAKR 623
Q Y+ GGLLFK+ SNMQ+VT SFLLL Y+NYL A VSCG + +P +LR +A+R
Sbjct: 192 QTTYTRGGLLFKLSDSNMQYVTSSSFLLLTYANYLVSAKKTVSCGGVAVTPQRLRAIARR 371
Query: 622 QVDYILGDNPLRMSYMVGYGARFPRRIHHRA 530
VDY+LG NP+ MSYMVGYGA++P+++HHRA
Sbjct: 372 MVDYLLGSNPMGMSYMVGYGAKYPKKLHHRA 464
Score = 48.7 bits (100), Expect(2) = 1e-040
Identities = 18/39 (46%), Positives = 26/39 (66%)
Frame = -1 / +2
Query: 952 NEFGWDNKHAGINVLISKEVLMGKDEYFQSFRVNADNFM 836
N FGWDNKHAG +L+SK L+ Q ++ +AD+F+
Sbjct: 44 NTFGWDNKHAGARILLSKSFLVH*VGALQEYKAHADSFI 160
>gb|BQ664895.1|BQ664895 HX01E21w HX Hordeum vulgare subsp. vulgare cDNA clone HX01E21
3-PRIME, mRNA sequence
Length = 513
Score = 164 bits (352), Expect = 1e-039
Identities = 67/78 (85%), Positives = 71/78 (91%)
Frame = -1 / -1
Query: 508 AHPARIGCKAGAAYYTSPAPNPNLLVGAVVGGPTDDSDAFPDARAVFQQSEPTTYINAPL 329
AHPARIGCKAGAAYY S APNPNLLVGAVVGGP++ SD FPDARAVFQQSEPTTYINAPL
Sbjct: 513 AHPARIGCKAGAAYYGSSAPNPNLLVGAVVGGPSNTSDNFPDARAVFQQSEPTTYINAPL 334
Query: 328 MGLLAYFSAHPNPAESGG 275
+GLLAYFSAHPN +S G
Sbjct: 333 LGLLAYFSAHPNLPQSDG 280
Score = 105 bits (225), Expect = 4e-022
Identities = 51/102 (50%), Positives = 58/102 (56%)
Frame = +3 / +2
Query: 201 PKPI*NTQARACVRHLSLSLGFFQSPPDSAGLGCAEK*ARRPMSGALMYVVGSDCWNTAR 380
P+P+ C + ++ + P D LG AEK*ARRP SGALM VVGSDCW TAR
Sbjct: 206 PRPLKRATPPHCPQRVAGHIYLQSVPSDWGRLGWAEK*ARRPSSGALM*VVGSDCWKTAR 385
Query: 381 ASGKASESSVGPPTTAPTSRFXXXXXXXXXXXXXXQPIRAGC 506
ASGK S +GPPTTAPTSR PIRAGC
Sbjct: 386 ASGKLSLVLLGPPTTAPTSRLGLGALLP*YAAPALHPIRAGC 511
>gb|BF256953.2|BF256953 HVSMEf0011H11f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0011H11f, mRNA sequence
Length = 819
Score = 154 bits (331), Expect = 9e-037
Identities = 66/183 (36%), Positives = 104/183 (56%)
Frame = -1 / +3
Query: 799 IQYSPGGLLFKVGSSNMQHVTQLSFLLLAYSNYLSHAGGRVSCGSSSASPVQLRRVAKRQ 620
++ +PGGLL+ +NMQ+V+ FLL Y++YL+ +G ++ C +P ++ A+ Q
Sbjct: 78 VKTTPGGLLYVADWNNMQYVSSSVFLLTVYADYLAESGDKLKCPDGEVAPAEIVAFARSQ 257
Query: 619 VDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHPARIGCKAGAAYYTSPAPNPN 440
VDY+LG NPL MSYMVG+ +FP +HHR +S+PSV A +GC G Y +
Sbjct: 258 VDYVLGKNPLSMSYMVGHSDKFPTHVHHRGASIPSVYAVIDTVGCMEGFDAYYNSKGADP 437
Query: 439 LLVGAVVGGPTDDSDAFPDARAVFQQSEPTTYINAPLMGLLAYFSAHPNPAESGGD*KKP 260
++ + + G D D F D R +Q++EPT AP+ G+ +A P + + P
Sbjct: 438 NVLVSALVGGPDRHDGFGDDRCSYQRAEPTLAAGAPMCGVFGRLAAEPAGGGNSRGYQPP 617
Query: 259 RES 251
+ES
Sbjct: 618 QES 626
>gb|CK125884.1|CK125884 BES1824111A2a04 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010A0411 5-PRIME, mRNA sequence
Length = 748
Score = 125 bits (268), Expect(2) = 2e-034
Identities = 55/126 (43%), Positives = 76/126 (60%)
Frame = -1 / +2
Query: 679 VSCGSSSASPVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHP 500
V C + +ASP ++ AK QVDYILG NP SYMVGYG +P HHR +S+ SV ++P
Sbjct: 194 VRCPAGAASPAEIMAFAKSQVDYILGSNPRGTSYMVGYGFIYPLEAHHRGASIVSVKSNP 373
Query: 499 ARIGCKAGAAYYTSPAPNPNLLVGAVVGGPTDDSDAFPDARAVFQQSEPTTYINAPLMGL 320
A + C+ G A + + L+ + G D+ D F D R ++Q+E TTY NAP MG+
Sbjct: 374 AFVSCRGGYATWYPRKGSNPNLLDGAIVGGPDEYDNFADERNNYEQTEATTYNNAPFMGV 553
Query: 319 LAYFSA 302
LA +A
Sbjct: 554 LARLAA 571
Score = 40.9 bits (83), Expect(2) = 2e-034
Identities = 19/38 (50%), Positives = 26/38 (68%)
Frame = -1 / +2
Query: 799 IQYSPGGLLFKVGSSNMQHVTQLSFLLLAYSNYLSHAG 686
++ +PGGLL +N+Q VT SFLL AYS+ L+ AG
Sbjct: 71 VKRTPGGLLHHQKWNNLQFVTSGSFLLAAYSDSLAAAG 184
>gb|BF259829.2|BF259829 HVSMEf0020G01f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020G01f, mRNA sequence
Length = 534
Score = 124 bits (266), Expect(2) = 2e-033
Identities = 48/112 (42%), Positives = 72/112 (64%)
Frame = -1 / +3
Query: 799 IQYSPGGLLFKVGSSNMQHVTQLSFLLLAYSNYLSHAGGRVSCGSSSASPVQLRRVAKRQ 620
++ +PGGLL+ +NMQ+V+ FLL Y++YL+ +G ++ C +P ++ A+ Q
Sbjct: 78 VKTTPGGLLYVADWNNMQYVSSSVFLLTVYADYLAESGDKLKCPDGEVAPAEIVAFARSQ 257
Query: 619 VDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHPARIGCKAGAAYY 464
VDY+LG NPL MSYMVG+ +FP +HHR +S+PSV A +GC G Y
Sbjct: 258 VDYVLGKNPLSMSYMVGHSDKFPTHVHHRGASIPSVYAVIDTVGCMEGFDAY 413
Score = 38.2 bits (77), Expect(2) = 2e-033
Identities = 14/21 (66%), Positives = 17/21 (80%)
Frame = -1 / +3
Query: 472 AYYTSPAPNPNLLVGAVVGGP 410
AYY S +PN+LVGA+VGGP
Sbjct: 408 AYYNSKGADPNVLVGALVGGP 470
>gb|AJ461943.1|AJ461943 AJ461943 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200058H03F1, mRNA sequence
Length = 300
Score = 132 bits (282), Expect = 5e-030
Identities = 54/65 (83%), Positives = 58/65 (89%)
Frame = -1 / +2
Query: 469 YYTSPAPNPNLLVGAVVGGPTDDSDAFPDARAVFQQSEPTTYINAPLMGLLAYFSAHPNP 290
YY S APNPNLLVGAVVGGP++ SD FPDARAVFQQSEPTTYINAPL+GLLAYFSAHPN
Sbjct: 2 YYGSSAPNPNLLVGAVVGGPSNTSDNFPDARAVFQQSEPTTYINAPLLGLLAYFSAHPNL 181
Query: 289 AESGG 275
+S G
Sbjct: 182 PQSDG 196
>gb|CA027804.1|CA027804 HZ60B10r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ60B10
5-PRIME, mRNA sequence
Length = 408
Score = 93.6 bits (198), Expect(2) = 8e-029
Identities = 36/57 (63%), Positives = 44/57 (77%)
Frame = -1 / +3
Query: 571 GYGARFPRRIHHRASSLPSVAAHPARIGCKAGAAYYTSPAPNPNLLVGAVVGGPTDD 401
GYGA++P+++HHRASSLPSVAAHP +IGC G S A NPN+ VGAVVGGP +
Sbjct: 12 GYGAKYPKKLHHRASSLPSVAAHPGKIGCSQGFTGLYSGAANPNVHVGAVVGGPNQN 182
Score = 54.2 bits (112), Expect(2) = 8e-029
Identities = 21/39 (53%), Positives = 28/39 (71%)
Frame = -1 / +3
Query: 421 VGGPTDDSDAFPDARAVFQQSEPTTYINAPLMGLLAYFS 305
V G + +D FPD R+ ++ SEP TYINAPL+G LAY +
Sbjct: 159 VVGGPNQNDQFPDQRSDYEHSEPATYINAPLVGALAYLA 275
>gb|CK122715.1|CK122715 BES1824105i22 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010I225 5-PRIME, mRNA sequence
Length = 776
Score = 125 bits (268), Expect = 4e-028
Identities = 55/126 (43%), Positives = 76/126 (60%)
Frame = -1 / +2
Query: 679 VSCGSSSASPVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHP 500
V C + +ASP ++ AK QVDYILG NP SYMVGYG +P HHR +S+ SV ++P
Sbjct: 8 VRCPAGAASPAEIMAFAKSQVDYILGSNPRGTSYMVGYGFIYPLEAHHRGASIVSVKSNP 187
Query: 499 ARIGCKAGAAYYTSPAPNPNLLVGAVVGGPTDDSDAFPDARAVFQQSEPTTYINAPLMGL 320
A + C+ G A + + L+ + G D+ D F D R ++Q+E TTY NAP MG+
Sbjct: 188 AFVSCRGGYATWYPRKGSNPNLLDGAIVGGPDEYDNFADERNNYEQTEATTYNNAPFMGV 367
Query: 319 LAYFSA 302
LA +A
Sbjct: 368 LARLAA 385
>gb|CK123273.1|CK123273 BES1824103c09 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010C093 5-PRIME, mRNA sequence
Length = 594
Score = 125 bits (268), Expect = 4e-028
Identities = 55/126 (43%), Positives = 76/126 (60%)
Frame = -1 / +2
Query: 679 VSCGSSSASPVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHP 500
V C + +ASP ++ AK QVDYILG NP SYMVGYG +P HHR +S+ SV ++P
Sbjct: 65 VRCPAGAASPAEIMAFAKSQVDYILGSNPRGTSYMVGYGFIYPLEAHHRGASIVSVKSNP 244
Query: 499 ARIGCKAGAAYYTSPAPNPNLLVGAVVGGPTDDSDAFPDARAVFQQSEPTTYINAPLMGL 320
A + C+ G A + + L+ + G D+ D F D R ++Q+E TTY NAP MG+
Sbjct: 245 AFVSCRGGYATWYPRKGSNPNLLDGAIVGGPDEYDNFADERNNYEQTEATTYNNAPFMGV 424
Query: 319 LAYFSA 302
LA +A
Sbjct: 425 LARLAA 442
>gb|BG369061.1|BG369061 HVSMEi0021J08f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0021J08f, mRNA sequence
Length = 797
Score = 81.3 bits (171), Expect(3) = 4e-027
Identities = 32/59 (54%), Positives = 42/59 (71%)
Frame = -1 / +3
Query: 652 PVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHPARIGCKAG 476
P +L +A+ Q +YILG NPLR+SYMVGYG R+P +HHRA+S+ S A+ IGC G
Sbjct: 204 PSELLALARSQAEYILGRNPLRLSYMVGYGPRYPAHVHHRAASIVSHKANNRFIGCMQG 380
Score = 44.1 bits (90), Expect(3) = 4e-027
Identities = 17/44 (38%), Positives = 28/44 (63%)
Frame = -1 / +3
Query: 817 ISNHPQIQYSPGGLLFKVGSSNMQHVTQLSFLLLAYSNYLSHAG 686
++N + SPGG+L+ +N+Q+ + +FLL YS YL+ AG
Sbjct: 30 LNNGSNVDRSPGGMLYVRQWNNLQYASSAAFLLTVYSRYLAGAG 161
Score = 34.5 bits (69), Expect(3) = 4e-027
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = -1 / +3
Query: 469 YYTSPAPNPNLLVGAVVGGP 410
++ PNPN+L GA+VGGP
Sbjct: 390 WFARKRPNPNVLTGAIVGGP 449
>gb|BU995458.1|BU995458 HM10F02r HM Hordeum vulgare subsp. vulgare cDNA clone HM10F02
5-PRIME, mRNA sequence
Length = 628
Score = 81.3 bits (171), Expect(3) = 4e-027
Identities = 32/59 (54%), Positives = 42/59 (71%)
Frame = -1 / +1
Query: 652 PVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHPARIGCKAG 476
P +L +A+ Q +YILG NPLR+SYMVGYG R+P +HHRA+S+ S A+ IGC G
Sbjct: 208 PSELLALARSQAEYILGRNPLRLSYMVGYGPRYPAHVHHRAASIVSHKANNRFIGCMQG 384
Score = 44.1 bits (90), Expect(3) = 4e-027
Identities = 17/44 (38%), Positives = 28/44 (63%)
Frame = -1 / +1
Query: 817 ISNHPQIQYSPGGLLFKVGSSNMQHVTQLSFLLLAYSNYLSHAG 686
++N + SPGG+L+ +N+Q+ + +FLL YS YL+ AG
Sbjct: 34 LNNGSNVDRSPGGMLYVRQWNNLQYASSAAFLLTVYSRYLAGAG 165
Score = 34.5 bits (69), Expect(3) = 4e-027
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = -1 / +1
Query: 469 YYTSPAPNPNLLVGAVVGGP 410
++ PNPN+L GA+VGGP
Sbjct: 394 WFARKRPNPNVLTGAIVGGP 453
>gb|BJ464625.1|BJ464625 BJ464625 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags35i04 5', mRNA sequence
Length = 385
Score = 85.4 bits (180), Expect(3) = 7e-027
Identities = 34/60 (56%), Positives = 43/60 (71%)
Frame = -1 / +3
Query: 1039 AWLHKATRRREYREYIKRNEVVLGASDAINEFGWDNKHAGINVLISKEVLMGKDEYFQSF 860
AWL +A+ +REYI+ N LGA D+INEFGWDNKHA +NVLISKE + G+ QS+
Sbjct: 201 AWLRRASGDDTFREYIQNNGKTLGAEDSINEFGWDNKHAXLNVLISKEFIXGEALXMQSY 380
Score = 40.5 bits (82), Expect(3) = 7e-027
Identities = 14/20 (70%), Positives = 16/20 (80%)
Frame = -2 / +3
Query: 1110 LHAAVCPCYCDYSGYQDELL 1051
L A CP YCD++GYQDELL
Sbjct: 132 LRAGGCPFYCDFNGYQDELL 191
Score = 33.1 bits (66), Expect(3) = 7e-027
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -2 / +3
Query: 1188 YSKRLLDRAVAVFEFADKYRGPYSSSLHAAVCPC 1087
Y++ LL+ A F+FAD +RG YS + C
Sbjct: 48 YAELLLESAKKAFDFADTHRGAYSDDMDLRAGGC 149
>gb|BQ766009.1|BQ766009 EBro03_SQ008_D24_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ008_D24 5', mRNA sequence
Length = 613
Score = 93.2 bits (197), Expect(2) = 4e-026
Identities = 40/115 (34%), Positives = 63/115 (54%)
Frame = -1 / +2
Query: 670 GSSSASPVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHPARI 491
G +A ++ +A+ QVDY+LG NP +SY+VGYGA++P R+HHRA+S+ I
Sbjct: 269 GGETAGAGEVAALARAQVDYVLGSNPRGVSYLVGYGAKYPARVHHRAASIVPYKHSKQFI 448
Query: 490 GCKAGAAYYTSPAPNPNLLVGAVVGGPTDDSDAFPDARAVFQQSEPTTYINAPLM 326
GC G ++ + ++ + G D D F D R + Q+E TY P++
Sbjct: 449 GCTQGFDHWFGRRSSNPNVLVGAIVGGPDRRDRFRDNRDNYMQTEACTYNTVPMV 613
Score = 45.5 bits (93), Expect(2) = 4e-026
Identities = 18/38 (47%), Positives = 28/38 (73%)
Frame = -1 / +2
Query: 799 IQYSPGGLLFKVGSSNMQHVTQLSFLLLAYSNYLSHAG 686
++ SPGG+L+ +NMQ+VT +FLL Y++YL+ AG
Sbjct: 134 VERSPGGMLYIRQWNNMQYVTNAAFLLSTYADYLAGAG 247
>gb|BM377965.2|BM377965 EBem04_SQ004_L21_R embryo, 12 DPA, no treatment, cv Optic, EBem04
Hordeum vulgare subsp. vulgare cDNA clone
EBem04_SQ004_L21 5', mRNA sequence
Length = 477
Score = 116 bits (247), Expect = 3e-025
Identities = 48/55 (87%), Positives = 49/55 (89%)
Frame = -1 / +3
Query: 424 VVGGPTDDSDAFPDARAVFQQSEPTTYINAPLMGLLAYFSAHPNPAESGGD*KKP 260
VVGGPTD +DAFPDARAVFQQSEPTTYINAPLMGLLAYFSAHPNPAE D* P
Sbjct: 3 VVGGPTDATDAFPDARAVFQQSEPTTYINAPLMGLLAYFSAHPNPAEWADD*HSP 167
Score = 102 bits (217), Expect = 5e-021
Identities = 44/51 (86%), Positives = 45/51 (88%)
Frame = +3 / -3
Query: 273 SPPDSAGLGCAEK*ARRPMSGALMYVVGSDCWNTARASGKASESSVGPPTT 425
S SAGLG AEK*ARRPM GALMYVVGSDCWNTARASGKAS +SVGPPTT
Sbjct: 154 SSAHSAGLGWAEK*ARRPMRGALMYVVGSDCWNTARASGKASVASVGPPTT 2
>gb|BI956376.1|BI956376 HVSMEn0002P20f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0002P20f, mRNA sequence
Length = 829
Score = 115 bits (245), Expect = 6e-025
Identities = 48/63 (76%), Positives = 50/63 (79%)
Frame = -1 / +1
Query: 595 PLRMSYMVGYGARFPRRIHHRASSLPSVAAHPARIGCKAGAAYYTSPAPNPNLLVGAVVG 416
PLRMSYMVGYG RFPRRIHHR SS+PSVAAHPARIG KAGAAYY + AP NLL G
Sbjct: 1 PLRMSYMVGYGPRFPRRIHHRGSSIPSVAAHPARIGSKAGAAYYATAAPKQNLLAENSGG 180
Query: 415 GPT 407
G T
Sbjct: 181 GVT 189
>gb|BI960651.1|BI960651 HVSMEn0025K02f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0025K02f, mRNA sequence
Length = 632
Score = 88.1 bits (186), Expect(2) = 1e-024
Identities = 38/114 (33%), Positives = 60/114 (52%)
Frame = -1 / +3
Query: 670 GSSSASPVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHPARI 491
G +A ++ +A+ QVDY+LG NP +SY+VGYGA++P R+HHRA+S+ I
Sbjct: 195 GGETAGAGEVAALARAQVDYVLGSNPRGVSYLVGYGAKYPARVHHRAASIVPYKHSKQFI 374
Query: 490 GCKAGAAYYTSPAPNPNLLVGAVVGGPTDDSDAFPDARAVFQQSEPTTYINAPL 329
GC G ++ + ++ + G D D F D R + +E T P+
Sbjct: 375 GCTQGFXHWFXRXSSNPNVLVGAIVGGPDREDRFRDNRDNYMHTEACTCKTGPM 536
Score = 45.5 bits (93), Expect(2) = 1e-024
Identities = 18/38 (47%), Positives = 28/38 (73%)
Frame = -1 / +3
Query: 799 IQYSPGGLLFKVGSSNMQHVTQLSFLLLAYSNYLSHAG 686
++ SPGG+L+ +NMQ+VT +FLL Y++YL+ AG
Sbjct: 60 VERSPGGMLYIRQWNNMQYVTNAAFLLSTYADYLAGAG 173
>gb|BQ460303.1|BQ460303 HA06K13r HA Hordeum vulgare subsp. vulgare cDNA clone HA06K13
5-PRIME, mRNA sequence
Length = 581
Score = 112 bits (240), Expect = 3e-024
Identities = 50/110 (45%), Positives = 67/110 (60%)
Frame = -1 / +1
Query: 631 AKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHPARIGCKAGAAYYTSPA 452
AK QVDYILG NP SYMVGYG +P HHR +S+ SV ++PA + C+ G A +
Sbjct: 25 AKSQVDYILGSNPRGTSYMVGYGFIYPLEAHHRGASIVSVKSNPAFVSCRGGYATWYPRK 204
Query: 451 PNPNLLVGAVVGGPTDDSDAFPDARAVFQQSEPTTYINAPLMGLLAYFSA 302
+ L+ + G D+ D F D R ++Q+E TTY NAP MG+LA +A
Sbjct: 205 GSNPNLLDGAIVGGPDEYDNFADERNNYEQTEATTYNNAPFMGVLARLAA 354
>gb|CK125396.1|CK125396 BES1824107o06 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010O067 5-PRIME, mRNA sequence
Length = 745
Score = 86.3 bits (182), Expect(2) = 1e-022
Identities = 34/70 (48%), Positives = 45/70 (64%)
Frame = -1 / +2
Query: 679 VSCGSSSASPVQLRRVAKRQVDYILGDNPLRMSYMVGYGARFPRRIHHRASSLPSVAAHP 500
V C + +ASP ++ AK QVDYILG NP SYMVGYG +P HHR +S+ SV ++P
Sbjct: 425 VRCPAGAASPAEIMAFAKSQVDYILGSNPRGTSYMVGYGFIYPLEAHHRGASIVSVKSNP 604
Query: 499 ARIGCKAGAA 470
+ C+ G A
Sbjct: 605 XFVSCRGGYA 634
Score = 40.9 bits (83), Expect(2) = 1e-022
Identities = 19/38 (50%), Positives = 26/38 (68%)
Frame = -1 / +2
Query: 799 IQYSPGGLLFKVGSSNMQHVTQLSFLLLAYSNYLSHAG 686
++ +PGGLL +N+Q VT SFLL AYS+ L+ AG
Sbjct: 302 VKRTPGGLLHHQKWNNLQFVTSGSFLLAAYSDSLAAAG 415
>gb|CB858324.1|CB858324 HI06J16w HI Hordeum vulgare subsp. vulgare cDNA clone HI06J16
3-PRIME, mRNA sequence
Length = 373
Score = 106 bits (227), Expect = 2e-022
Identities = 44/51 (86%), Positives = 45/51 (88%)
Frame = -1 / -1
Query: 412 PTDDSDAFPDARAVFQQSEPTTYINAPLMGLLAYFSAHPNPAESGGD*KKP 260
PTD +DAFPDARAVFQQSEPTTYINAPLMGLLAYFSAHPNPAE D* P
Sbjct: 373 PTDATDAFPDARAVFQQSEPTTYINAPLMGLLAYFSAHPNPAEWADD*HSP 221
>gb|BU996208.1|BU996208 HM12N12r HM Hordeum vulgare subsp. vulgare cDNA clone HM12N12
5-PRIME, mRNA sequence
Length = 422
Score = 63.8 bits (133), Expect(2) = 5e-022
Identities = 24/42 (57%), Positives = 33/42 (78%)
Frame = -1 / +1
Query: 415 GPTDDSDAFPDARAVFQQSEPTTYINAPLMGLLAYFSAHPNP 290
G + D++ D RA +++SEPTTYINAPL+G+LAYF +PNP
Sbjct: 121 GGPGEDDSYGDDRADYRKSEPTTYINAPLVGVLAYFVGNPNP 246
Score = 61.1 bits (127), Expect(2) = 5e-022
Identities = 22/35 (62%), Positives = 26/35 (74%)
Frame = -1 / +1
Query: 505 HPARIGCKAGAAYYTSPAPNPNLLVGAVVGGPTDD 401
HP R+ CK G YY S +PNPN LVGA+VGGP +D
Sbjct: 34 HPERMACKDGTPYYDSSSPNPNPLVGAIVGGPGED 138
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 453,102,164
Number of Sequences: 312970
Number of extensions: 8063269
Number of successful extensions: 84225
Number of sequences better than 1.0e-020: 34
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 14752141457
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)