TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2550809.2.1
(876 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA031523.1|CA031523 HX10D23r HX Hordeum vulgare subsp. v... 347 7e-095
gb|BI780944.2|BI780944 EBma05_SQ001_J17_R maternal, 12 DPA,... 303 1e-081
gb|BI776406.2|BI776406 EBpi05_SQ001_E05_R pistil, 8 DPA, no... 287 4e-077
gb|BU970562.1|BU970562 HB15A07r BC Hordeum vulgare subsp. v... 268 3e-071
gb|BQ765772.1|BQ765772 EBro03_SQ008_J04_R root, 3 week, wat... 259 2e-068
gb|CB876258.1|CB876258 HX10M02w HX Hordeum vulgare subsp. v... 242 3e-063
gb|BM100842.2|BM100842 EBpi01_SQ002_C12_R pistil, 1 DPA, no... 188 3e-047
gb|BQ754697.1|BQ754697 EBed01_SQ003_I23_R endosperm, 6 DPA,... 166 2e-040
>gb|CA031523.1|CA031523 HX10D23r HX Hordeum vulgare subsp. vulgare cDNA clone HX10D23
5-PRIME, mRNA sequence
Length = 622
Score = 347 bits (751), Expect = 7e-095
Identities = 140/170 (82%), Positives = 161/170 (94%)
Frame = -1 / +3
Query: 780 MVFLEAEMSWNVLISPSQLDRKGLLLRKAIIVRLLEDVTHRRASKEHGYYIAVNQLKAIS 601
MVFL+ EMSWNVLISP QL KGLLLRK+IIVRLLED+T+R+AS EHGYYIAVN+LK IS
Sbjct: 111 MVFLQEEMSWNVLISPDQLSPKGLLLRKSIIVRLLEDITNRKASNEHGYYIAVNELKTIS 290
Query: 600 EGKVRELTGDVLFPVSFTCITQKPMKGEVMVGHVDRILKHGVFLKSGPVESIFLAEKSMS 421
EGKVRELTGDVLFPV+FTCITQ+PMKGE++VG V++ILKHGVFLKSGP+ESIFL+EKSMS
Sbjct: 291 EGKVRELTGDVLFPVTFTCITQRPMKGEILVGSVEKILKHGVFLKSGPIESIFLSEKSMS 470
Query: 420 NYKYIGGENAMFMNDHSKLEKDTAVRFKVLGFRWMEADRQFQLLATIAGD 271
+YKY+GGEN MFM DHSKLE+DTAVRFKV+GFRWMEA+RQFQLLA++AGD
Sbjct: 471 DYKYMGGENPMFMKDHSKLERDTAVRFKVMGFRWMEAERQFQLLASLAGD 620
Score = 283 bits (613), Expect = 8e-076
Identities = 129/171 (75%), Positives = 133/171 (77%)
Frame = +2 / -1
Query: 269 KSPAIVARSWNWRSASIQRNPRTLKRTAVSFSSFEWSFMNIAFSPPMYL*LLIDFSARKM 448
KSPA ARSWNWRSASIQRNP TLKRTAVS SSFEWSFMN+ FSPPMYL* LIDFS RKM
Sbjct: 622 KSPASDARSWNWRSASIQRNPMTLKRTAVSLSSFEWSFMNMGFSPPMYL*SLIDFSDRKM 443
Query: 449 LSTGPDLRNTPCLRILSTCPTMTSPFIGFWVMQVNETGNRTSPVSSRTFPSDIAFS*LTA 628
LS GPDLR TPCLRI ST PT SPFIG VMQVN TG RTSPVSSRTFPS+I FS LTA
Sbjct: 442 LSIGPDLRKTPCLRIFSTEPTKISPFIGL*VMQVNVTGKRTSPVSSRTFPSEIVFSSLTA 263
Query: 629 M**PCSFEALLCVTSSRRRTMIAXXXXXXXXXXXXGEINTFHDISASRKTM 781
M**PCS EA L V SSRRRTMI GEI+TF DIS+ RKTM
Sbjct: 262 M**PCSLEAFLLVMSSRRRTMIDLRSSRPLGLSWSGEISTFQDISS*RKTM 110
Score = 211 bits (455), Expect = 5e-054
Identities = 96/170 (56%), Positives = 116/170 (68%)
Frame = +1 / -3
Query: 271 VTSDRSKELELAVSLHPAEP*DLEAHGGVLLQLRVVVHEHRILPTYVLVITHRLLSQEDA 450
+TS R KELELA LHPAEP DLEAH GV LQL VV+HEH +L +VLV+ HRLL QEDA
Sbjct: 620 ITSQRRKELELAFCLHPAEPHDLEAHSGVPLQL*VVLHEHGVLAAHVLVVAHRLLRQEDA 441
Query: 451 LHGSGFEEHAMLEDPVHVPNHDLSLHRLLGNASK*NWEQNISS*LTHFPFRYCLQLINSN 630
L GFEE +LED +H + DL LHR L +A + + E+N+S T+ PFR LQL++SN
Sbjct: 440 LDWPGFEEDPVLEDLLHGADQDLPLHRPLSDAGECDREKNVSGQFTNLPFRDRLQLVDSN 261
Query: 631 VVAMLFRSPPVCDILQKTHNDSXXXXXXXXXXXXR*DQHIP*HLCLKKNH 780
VVAML PV D+LQKTH+D R*DQH+P HL LK+NH
Sbjct: 260 VVAMLIGGLPVGDVLQKTHDDRLAEQQALGAQLVR*DQHVPRHLLLKENH 111
Score = 190 bits (409), Expect(2) = 4e-049
Identities = 86/140 (61%), Positives = 97/140 (69%)
Frame = -3 / +2
Query: 691 HCASSGGCHTQEGFERAWLLHCC*SAEGNI*RESA*ANWRCSVPSFIYLHYPEAYEGRGH 512
H ASSGG H QEG + AWLLHCC AE ++*RE +* + R S P I+LH+ EAYEG
Sbjct: 200 HRASSGGHHQQEGLQ*AWLLHCCQRAEDDL*REGS*TDRRRSFPGHIHLHHSEAYEGGDL 379
Query: 511 GWARGQDPQAWRVPQIRTRGEHLPG*EVDE*LQVHRWGECDVHERPLEAGEGHRRALQGP 332
G RG+DPQA +PQIR EHLP EVDE LQVH E VHE PL+AGEGHR ALQG
Sbjct: 380 GRLRGEDPQARGLPQIRANREHLPVGEVDERLQVHGRREPHVHEGPLKAGEGHRCALQGH 559
Query: 331 RVPLDGG*PPIPAPCYDRW* 272
VPLDGG P+PAPC W*
Sbjct: 560 GVPLDGGRTPVPAPCVAGW* 619
Score = 138 bits (296), Expect(2) = 3e-034
Identities = 72/131 (54%), Positives = 79/131 (60%)
Frame = -2 / +1
Query: 788 IQPWFFLRQRCHGMC*SHLASWTARASCSARLSLCVFWRMSHTGGLRKSMATTLLLIS*R 609
++ WF R+RC G C*SH S RA CSA S CVFWR S TG SMATTLL S*R
Sbjct: 103 VKTWFSFRRRCLGTC*SHRTS*APRACCSASRSSCVFWRTSPTGRPPMSMATTLLSTS*R 282
Query: 608 QYLKGKCVS*LEMFCSQFHLLALPRSL*RERSWLGTWTGSSSMACSSNPDPWRASSWLRS 429
+ LKG+ V+* E F S+ H A R L*R RSW W SSS SSNP RASS RS
Sbjct: 283 RSLKGRFVN*PETFFSRSHSPASLRGL*RGRSWSAPWRRSSSTGSSSNPGQSRASSCRRS 462
Query: 428 R*VITST*VGR 396
R* TST R
Sbjct: 463 R*ATTSTWAAR 495
Score = 111 bits (237), Expect(2) = 1e-027
Identities = 57/94 (60%), Positives = 64/94 (68%)
Frame = +3 / -2
Query: 408 CTCNYSSTSQPGRCSPRVRI*GTRHA*GSCPRAQP*PLPS*ASG*CK*MKLGTEHLQLAH 587
CTC+ SSTS GRCS RI*G A*GS PR++P PS*AS *C+*M G E L+ H
Sbjct: 483 CTCSRSSTSPTGRCSRLARI*GRPRA*GSSPRSRPRSPPS*ASE*CR*M*PGKERLRSVH 304
Query: 588 ALSLQILPSAD*QQCSSHALSKPSCV*HPPEDAQ 689
SLQ SA *QQCSSHA +PSC * PPEDA+
Sbjct: 303 EPSLQRSSSAR*QQCSSHAHWRPSCW*CPPEDAR 202
Score = 31.8 bits (63), Expect(2) = 1e-027
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = +3 / -2
Query: 270 SHQRS*QGAGIGGQPPSSGT 329
+HQ + QGAG G PPSSGT
Sbjct: 621 NHQPATQGAGTGVLPPSSGT 562
Score = 26.7 bits (52), Expect(2) = 3e-034
Identities = 11/15 (73%), Positives = 12/15 (80%)
Frame = -2 / +1
Query: 344 ASRS*GSAGWRLTAN 300
ASRS GSAGWR A+
Sbjct: 547 ASRSWGSAGWRQNAS 591
Score = 24.9 bits (48), Expect(2) = 4e-049
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -3 / +2
Query: 781 HGFS*GRDVMECVDLT 734
HGF G DV+E DLT
Sbjct: 110 HGFPSGGDVLERADLT 157
>gb|BI780944.2|BI780944 EBma05_SQ001_J17_R maternal, 12 DPA, no treatment, cv Optic, EBma05
Hordeum vulgare subsp. vulgare cDNA clone
EBma05_SQ001_J17 5', mRNA sequence
Length = 522
Score = 303 bits (655), Expect = 1e-081
Identities = 123/150 (82%), Positives = 141/150 (94%)
Frame = -1 / +2
Query: 780 MVFLEAEMSWNVLISPSQLDRKGLLLRKAIIVRLLEDVTHRRASKEHGYYIAVNQLKAIS 601
MVFL+ EMSWNVLISP QL KGLLLRK+IIVRLLED+T+R+AS EHGYYIAVN+LK IS
Sbjct: 71 MVFLQEEMSWNVLISPDQLSPKGLLLRKSIIVRLLEDITNRKASNEHGYYIAVNELKTIS 250
Query: 600 EGKVRELTGDVLFPVSFTCITQKPMKGEVMVGHVDRILKHGVFLKSGPVESIFLAEKSMS 421
EGKVRELTGDVLFPV+FTCITQ+PMKGE++VG V++ILKHGVFLKSGP+ESIFL+EKSMS
Sbjct: 251 EGKVRELTGDVLFPVTFTCITQRPMKGEILVGSVEKILKHGVFLKSGPIESIFLSEKSMS 430
Query: 420 NYKYIGGENAMFMNDHSKLEKDTAVRFKVL 331
+YKY+GGEN MFM DHSKLE+DTAVRFKV+
Sbjct: 431 DYKYMGGENPMFMKDHSKLERDTAVRFKVM 520
Score = 241 bits (520), Expect = 5e-063
Identities = 111/151 (73%), Positives = 115/151 (76%)
Frame = +2 / -1
Query: 329 PRTLKRTAVSFSSFEWSFMNIAFSPPMYL*LLIDFSARKMLSTGPDLRNTPCLRILSTCP 508
P TLKRTAVS SSFEWSFMN+ FSPPMYL* LIDFS RKMLS GPDLR TPCLRI ST P
Sbjct: 522 PMTLKRTAVSLSSFEWSFMNMGFSPPMYL*SLIDFSDRKMLSIGPDLRKTPCLRIFSTEP 343
Query: 509 TMTSPFIGFWVMQVNETGNRTSPVSSRTFPSDIAFS*LTAM**PCSFEALLCVTSSRRRT 688
T SPFIG VMQVN TG RTSPVSSRTFPS+I FS LTAM**PCS EA L V SSRRRT
Sbjct: 342 TKISPFIGL*VMQVNVTGKRTSPVSSRTFPSEIVFSSLTAM**PCSLEAFLLVMSSRRRT 163
Query: 689 MIAXXXXXXXXXXXXGEINTFHDISASRKTM 781
MI GEI+TF DIS+ RKTM
Sbjct: 162 MIDLRSSRPLGLSWSGEISTFQDISS*RKTM 70
Score = 178 bits (383), Expect = 4e-044
Identities = 81/149 (54%), Positives = 100/149 (67%)
Frame = +1 / -3
Query: 334 DLEAHGGVLLQLRVVVHEHRILPTYVLVITHRLLSQEDALHGSGFEEHAMLEDPVHVPNH 513
DLEAH GV LQL VV+HEH +L +VLV+ HRLL QEDAL GFEE +LED +H +
Sbjct: 517 DLEAHSGVPLQL*VVLHEHGVLAAHVLVVAHRLLRQEDALDWPGFEEDPVLEDLLHGADQ 338
Query: 514 DLSLHRLLGNASK*NWEQNISS*LTHFPFRYCLQLINSNVVAMLFRSPPVCDILQKTHND 693
DL LHR L +A + + E+N+S T+ PFR LQL++SNVVAML PV D+LQKTH+D
Sbjct: 337 DLPLHRPLSDAGECDREKNVSGQFTNLPFRDRLQLVDSNVVAMLIGGLPVGDVLQKTHDD 158
Query: 694 SXXXXXXXXXXXXR*DQHIP*HLCLKKNH 780
R*DQH+P HL LK+NH
Sbjct: 157 RLAEQQALGAQLVR*DQHVPRHLLLKENH 71
Score = 159 bits (341), Expect(2) = 9e-040
Identities = 73/119 (61%), Positives = 83/119 (69%)
Frame = -3 / +1
Query: 691 HCASSGGCHTQEGFERAWLLHCC*SAEGNI*RESA*ANWRCSVPSFIYLHYPEAYEGRGH 512
H ASSGG H QEG + AWLLHCC AE ++*RE +* + R S P I+LH+ EAYEG
Sbjct: 160 HRASSGGHHQQEGLQ*AWLLHCCQRAEDDL*REGS*TDRRRSFPGHIHLHHSEAYEGGDL 339
Query: 511 GWARGQDPQAWRVPQIRTRGEHLPG*EVDE*LQVHRWGECDVHERPLEAGEGHRRALQG 335
G RG+DPQA +PQIR EHLP EVDE LQVH E VHE PL+AGEGHR ALQG
Sbjct: 340 GRLRGEDPQARGLPQIRANREHLPVGEVDERLQVHGRREPHVHEGPLKAGEGHRCALQG 516
Score = 138 bits (296), Expect = 4e-032
Identities = 72/131 (54%), Positives = 79/131 (60%)
Frame = -2 / +3
Query: 788 IQPWFFLRQRCHGMC*SHLASWTARASCSARLSLCVFWRMSHTGGLRKSMATTLLLIS*R 609
++ WF R+RC G C*SH S RA CSA S CVFWR S TG SMATTLL S*R
Sbjct: 63 VKTWFSFRRRCLGTC*SHRTS*APRACCSASRSSCVFWRTSPTGRPPMSMATTLLSTS*R 242
Query: 608 QYLKGKCVS*LEMFCSQFHLLALPRSL*RERSWLGTWTGSSSMACSSNPDPWRASSWLRS 429
+ LKG+ V+* E F S+ H A R L*R RSW W SSS SSNP RASS RS
Sbjct: 243 RSLKGRFVN*PETFFSRSHSPASLRGL*RGRSWSAPWRRSSSTGSSSNPGQSRASSCRRS 422
Query: 428 R*VITST*VGR 396
R* TST R
Sbjct: 423 R*ATTSTWAAR 455
Score = 111 bits (237), Expect = 5e-024
Identities = 57/94 (60%), Positives = 64/94 (68%)
Frame = +3 / -2
Query: 408 CTCNYSSTSQPGRCSPRVRI*GTRHA*GSCPRAQP*PLPS*ASG*CK*MKLGTEHLQLAH 587
CTC+ SSTS GRCS RI*G A*GS PR++P PS*AS *C+*M G E L+ H
Sbjct: 443 CTCSRSSTSPTGRCSRLARI*GRPRA*GSSPRSRPRSPPS*ASE*CR*M*PGKERLRSVH 264
Query: 588 ALSLQILPSAD*QQCSSHALSKPSCV*HPPEDAQ 689
SLQ SA *QQCSSHA +PSC * PPEDA+
Sbjct: 263 EPSLQRSSSAR*QQCSSHAHWRPSCW*CPPEDAR 162
Score = 24.9 bits (48), Expect(2) = 9e-040
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -3 / +1
Query: 781 HGFS*GRDVMECVDLT 734
HGF G DV+E DLT
Sbjct: 70 HGFPSGGDVLERADLT 117
>gb|BI776406.2|BI776406 EBpi05_SQ001_E05_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ001_E05 5', mRNA sequence
Length = 488
Score = 287 bits (622), Expect = 4e-077
Identities = 115/138 (83%), Positives = 132/138 (95%)
Frame = -1 / +3
Query: 657 RASKEHGYYIAVNQLKAISEGKVRELTGDVLFPVSFTCITQKPMKGEVMVGHVDRILKHG 478
+AS EHGYYIAVN+LK ISEGKVRELTGDVLFPV+FTCITQ+PMKGE++VG V++ILKHG
Sbjct: 3 KASNEHGYYIAVNELKTISEGKVRELTGDVLFPVTFTCITQRPMKGEILVGSVEKILKHG 182
Query: 477 VFLKSGPVESIFLAEKSMSNYKYIGGENAMFMNDHSKLEKDTAVRFKVLGFRWMEADRQF 298
VFLKSGP+ESIFL+EKSMS+YKY+GGEN MFM DHSKLE+DTAVRFKV+GFRWMEA+RQF
Sbjct: 183 VFLKSGPIESIFLSEKSMSDYKYMGGENPMFMKDHSKLERDTAVRFKVMGFRWMEAERQF 362
Query: 297 QLLATIAGDFLGPL*TAS 244
QLLA++AGDFLGPL* AS
Sbjct: 363 QLLASLAGDFLGPL*AAS 416
Score = 260 bits (563), Expect = 6e-069
Identities = 114/137 (83%), Positives = 116/137 (84%)
Frame = +2 / -2
Query: 245 EAVHSGPRKSPAIVARSWNWRSASIQRNPRTLKRTAVSFSSFEWSFMNIAFSPPMYL*LL 424
EA HSGPRKSPA ARSWNWRSASIQRNP TLKRTAVS SSFEWSFMN+ FSPPMYL* L
Sbjct: 415 EAAHSGPRKSPASDARSWNWRSASIQRNPMTLKRTAVSLSSFEWSFMNMGFSPPMYL*SL 236
Query: 425 IDFSARKMLSTGPDLRNTPCLRILSTCPTMTSPFIGFWVMQVNETGNRTSPVSSRTFPSD 604
IDFS RKMLS GPDLR TPCLRI ST PT SPFIG VMQVN TG RTSPVSSRTFPS+
Sbjct: 235 IDFSDRKMLSIGPDLRKTPCLRIFSTEPTKISPFIGL*VMQVNVTGKRTSPVSSRTFPSE 56
Query: 605 IAFS*LTAM**PCSFEA 655
I FS LTAM**PCS EA
Sbjct: 55 IVFSSLTAM**PCSLEA 5
Score = 188 bits (405), Expect = 4e-047
Identities = 84/141 (59%), Positives = 98/141 (69%)
Frame = -3 / +2
Query: 658 EGFERAWLLHCC*SAEGNI*RESA*ANWRCSVPSFIYLHYPEAYEGRGHGWARGQDPQAW 479
EG + AWLLHCC AE ++*RE +* + R S P I+LH+ EAYEG G RG+DPQA
Sbjct: 2 EGLQ*AWLLHCCQRAEDDL*REGS*TDRRRSFPGHIHLHHSEAYEGGDLGRLRGEDPQAR 181
Query: 478 RVPQIRTRGEHLPG*EVDE*LQVHRWGECDVHERPLEAGEGHRRALQGPRVPLDGG*PPI 299
+PQIR EHLP EVDE LQVH E VHE PL+AGEGHR ALQG VPLDGG P+
Sbjct: 182 GLPQIRANREHLPVGEVDERLQVHGRREPHVHEGPLKAGEGHRCALQGHGVPLDGGRTPV 361
Query: 298 PAPCYDRW*LPWAAVNCFHRS 236
PAPC W* P AAV+CF ++
Sbjct: 362 PAPCVAGW*FPRAAVSCFEQA 424
Score = 180 bits (388), Expect = 8e-045
Identities = 82/133 (61%), Positives = 99/133 (74%)
Frame = +1 / -1
Query: 247 SSSQRPKEVTSDRSKELELAVSLHPAEP*DLEAHGGVLLQLRVVVHEHRILPTYVLVITH 426
SSSQRP+E+TS R KELELA LHPAEP DLEAH GV LQL VV+HEH +L +VLV+ H
Sbjct: 413 SSSQRPEEITSQRRKELELAFCLHPAEPHDLEAHSGVPLQL*VVLHEHGVLAAHVLVVAH 234
Query: 427 RLLSQEDALHGSGFEEHAMLEDPVHVPNHDLSLHRLLGNASK*NWEQNISS*LTHFPFRY 606
RLL QEDAL GFEE +LED +H + DL LHR L +A + + E+N+S T+ PFR
Sbjct: 233 RLLRQEDALDWPGFEEDPVLEDLLHGADQDLPLHRPLSDAGECDREKNVSGQFTNLPFRD 54
Query: 607 CLQLINSNVVAML 645
LQL++SNVVAML
Sbjct: 53 RLQLVDSNVVAML 15
Score = 95.9 bits (203), Expect(2) = 4e-027
Identities = 50/84 (59%), Positives = 56/84 (66%)
Frame = +3 / -3
Query: 408 CTCNYSSTSQPGRCSPRVRI*GTRHA*GSCPRAQP*PLPS*ASG*CK*MKLGTEHLQLAH 587
CTC+ SSTS GRCS RI*G A*GS PR++P PS*AS *C+*M G E L+ H
Sbjct: 252 CTCSRSSTSPTGRCSRLARI*GRPRA*GSSPRSRPRSPPS*ASE*CR*M*PGKERLRSVH 73
Query: 588 ALSLQILPSAD*QQCSSHALSKPS 659
SLQ SA *QQCSSHA +PS
Sbjct: 72 EPSLQRSSSAR*QQCSSHAHWRPS 1
Score = 82.6 bits (174), Expect(2) = 6e-021
Identities = 47/83 (56%), Positives = 51/83 (61%)
Frame = -2 / +1
Query: 644 SMATTLLLIS*RQYLKGKCVS*LEMFCSQFHLLALPRSL*RERSWLGTWTGSSSMACSSN 465
SMATTLL S*R+ LKG+ V+* E F S+ H A R L*R RSW W SSS SSN
Sbjct: 16 SMATTLLSTS*RRSLKGRFVN*PETFFSRSHSPASLRGL*RGRSWSAPWRRSSSTGSSSN 195
Query: 464 PDPWRASSWLRSR*VITST*VGR 396
P RASS RSR* TST R
Sbjct: 196 PGQSRASSCRRSR*ATTSTWAAR 264
Score = 45.5 bits (93), Expect(2) = 4e-027
Identities = 19/28 (67%), Positives = 22/28 (78%)
Frame = +3 / -3
Query: 246 KQFTAAQGSHQRS*QGAGIGGQPPSSGT 329
KQ TAA+G+HQ + QGAG G PPSSGT
Sbjct: 414 KQLTAARGNHQPATQGAGTGVLPPSSGT 331
Score = 38.2 bits (77), Expect(2) = 6e-021
Identities = 17/33 (51%), Positives = 18/33 (54%)
Frame = -2 / +1
Query: 344 ASRS*GSAGWRLTANXXXXXXXXXXXLGRCELL 246
ASRS GSAGWR A+ GRCELL
Sbjct: 316 ASRSWGSAGWRQNASSSSLRRWLVISSGRCELL 414
>gb|BU970562.1|BU970562 HB15A07r BC Hordeum vulgare subsp. vulgare cDNA clone HB15A07
5-PRIME, mRNA sequence
Length = 486
Score = 268 bits (580), Expect = 3e-071
Identities = 109/133 (81%), Positives = 125/133 (93%)
Frame = -1 / +1
Query: 780 MVFLEAEMSWNVLISPSQLDRKGLLLRKAIIVRLLEDVTHRRASKEHGYYIAVNQLKAIS 601
MVFL+ EMSWNVLISP QL KGLLLRK+IIVRLLED+T+R+AS EHGYYIAVN+LK IS
Sbjct: 88 MVFLQEEMSWNVLISPDQLSPKGLLLRKSIIVRLLEDITNRKASNEHGYYIAVNELKTIS 267
Query: 600 EGKVRELTGDVLFPVSFTCITQKPMKGEVMVGHVDRILKHGVFLKSGPVESIFLAEKSMS 421
EGKVRELTGDVLFPV+FTCITQ+PMKGE++VG V++ILKHGVFLKSGP+ESIFL+EKSMS
Sbjct: 268 EGKVRELTGDVLFPVTFTCITQRPMKGEILVGSVEKILKHGVFLKSGPIESIFLSEKSMS 447
Query: 420 NYKYIGGENAMFM 382
+YKY+GGEN MFM
Sbjct: 448 DYKYMGGENPMFM 486
Score = 203 bits (438), Expect = 1e-051
Identities = 95/133 (71%), Positives = 99/133 (74%)
Frame = +2 / -2
Query: 383 MNIAFSPPMYL*LLIDFSARKMLSTGPDLRNTPCLRILSTCPTMTSPFIGFWVMQVNETG 562
MN+ FSPPMYL* LIDFS RKMLS GPDLR TPCLRI ST PT SPFIG VMQVN TG
Sbjct: 485 MNMGFSPPMYL*SLIDFSDRKMLSIGPDLRKTPCLRIFSTEPTKISPFIGL*VMQVNVTG 306
Query: 563 NRTSPVSSRTFPSDIAFS*LTAM**PCSFEALLCVTSSRRRTMIAXXXXXXXXXXXXGEI 742
RTSPVSSRTFPS+I FS LTAM**PCS EA L V SSRRRTMI GEI
Sbjct: 305 KRTSPVSSRTFPSEIVFSSLTAM**PCSLEAFLLVMSSRRRTMIDLRSSRPLGLSWSGEI 126
Query: 743 NTFHDISASRKTM 781
+TF DIS+ RKTM
Sbjct: 125 STFQDISS*RKTM 87
Score = 154 bits (331), Expect = 6e-037
Identities = 69/133 (51%), Positives = 87/133 (65%)
Frame = +1 / -1
Query: 382 HEHRILPTYVLVITHRLLSQEDALHGSGFEEHAMLEDPVHVPNHDLSLHRLLGNASK*NW 561
HEH +L +VLV+ HRLL QEDAL GFEE +LED +H + DL LHR L +A + +
Sbjct: 486 HEHGVLAAHVLVVAHRLLRQEDALDWPGFEEDPVLEDLLHGADQDLPLHRPLSDAGECDR 307
Query: 562 EQNISS*LTHFPFRYCLQLINSNVVAMLFRSPPVCDILQKTHNDSXXXXXXXXXXXXR*D 741
E+N+S T+ PFR LQL++SNVVAML PV D+LQKTH+D R*D
Sbjct: 306 EKNVSGQFTNLPFRDRLQLVDSNVVAMLIGGLPVGDVLQKTHDDRLAEQQALGAQLVR*D 127
Query: 742 QHIP*HLCLKKNH 780
QH+P HL LK+NH
Sbjct: 126 QHVPRHLLLKENH 88
Score = 138 bits (296), Expect = 4e-032
Identities = 72/131 (54%), Positives = 79/131 (60%)
Frame = -2 / +2
Query: 788 IQPWFFLRQRCHGMC*SHLASWTARASCSARLSLCVFWRMSHTGGLRKSMATTLLLIS*R 609
++ WF R+RC G C*SH S RA CSA S CVFWR S TG SMATTLL S*R
Sbjct: 80 VKTWFSFRRRCLGTC*SHRTS*APRACCSASRSSCVFWRTSPTGRPPMSMATTLLSTS*R 259
Query: 608 QYLKGKCVS*LEMFCSQFHLLALPRSL*RERSWLGTWTGSSSMACSSNPDPWRASSWLRS 429
+ LKG+ V+* E F S+ H A R L*R RSW W SSS SSNP RASS RS
Sbjct: 260 RSLKGRFVN*PETFFSRSHSPASLRGL*RGRSWSAPWRRSSSTGSSSNPGQSRASSCRRS 439
Query: 428 R*VITST*VGR 396
R* TST R
Sbjct: 440 R*ATTSTWAAR 472
Score = 129 bits (276), Expect(2) = 6e-031
Identities = 60/103 (58%), Positives = 69/103 (66%)
Frame = -3 / +3
Query: 691 HCASSGGCHTQEGFERAWLLHCC*SAEGNI*RESA*ANWRCSVPSFIYLHYPEAYEGRGH 512
H ASSGG H QEG + AWLLHCC AE ++*RE +* + R S P I+LH+ EAYEG
Sbjct: 177 HRASSGGHHQQEGLQ*AWLLHCCQRAEDDL*REGS*TDRRRSFPGHIHLHHSEAYEGGDL 356
Query: 511 GWARGQDPQAWRVPQIRTRGEHLPG*EVDE*LQVHRWGECDVH 383
G RG+DPQA +PQIR EHLP EVDE LQVH E VH
Sbjct: 357 GRLRGEDPQARGLPQIRANREHLPVGEVDERLQVHGRREPHVH 485
Score = 111 bits (237), Expect = 5e-024
Identities = 57/94 (60%), Positives = 64/94 (68%)
Frame = +3 / -3
Query: 408 CTCNYSSTSQPGRCSPRVRI*GTRHA*GSCPRAQP*PLPS*ASG*CK*MKLGTEHLQLAH 587
CTC+ SSTS GRCS RI*G A*GS PR++P PS*AS *C+*M G E L+ H
Sbjct: 460 CTCSRSSTSPTGRCSRLARI*GRPRA*GSSPRSRPRSPPS*ASE*CR*M*PGKERLRSVH 281
Query: 588 ALSLQILPSAD*QQCSSHALSKPSCV*HPPEDAQ 689
SLQ SA *QQCSSHA +PSC * PPEDA+
Sbjct: 280 EPSLQRSSSAR*QQCSSHAHWRPSCW*CPPEDAR 179
Score = 24.9 bits (48), Expect(2) = 6e-031
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -3 / +3
Query: 781 HGFS*GRDVMECVDLT 734
HGF G DV+E DLT
Sbjct: 87 HGFPSGGDVLERADLT 134
>gb|BQ765772.1|BQ765772 EBro03_SQ008_J04_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ008_J04 5', mRNA sequence
Length = 591
Score = 259 bits (559), Expect = 2e-068
Identities = 104/124 (83%), Positives = 119/124 (95%)
Frame = -1 / +2
Query: 615 LKAISEGKVRELTGDVLFPVSFTCITQKPMKGEVMVGHVDRILKHGVFLKSGPVESIFLA 436
LK ISEGKVRELTGDVLFPV+FTCITQ+PMKGE++VG V++ILKHGVFLKSGP+ESIFL+
Sbjct: 2 LKTISEGKVRELTGDVLFPVTFTCITQRPMKGEILVGSVEKILKHGVFLKSGPIESIFLS 181
Query: 435 EKSMSNYKYIGGENAMFMNDHSKLEKDTAVRFKVLGFRWMEADRQFQLLATIAGDFLGPL 256
EKSMS+YKY+GGEN MFM DHSKLE+DTAVRFKV+GFRWMEA+RQFQLLA++AGDFLGPL
Sbjct: 182 EKSMSDYKYMGGENPMFMKDHSKLERDTAVRFKVMGFRWMEAERQFQLLASLAGDFLGPL 361
Query: 255 *TAS 244
* AS
Sbjct: 362 *AAS 373
Score = 240 bits (518), Expect = 1e-062
Identities = 103/124 (83%), Positives = 105/124 (84%)
Frame = +2 / -1
Query: 245 EAVHSGPRKSPAIVARSWNWRSASIQRNPRTLKRTAVSFSSFEWSFMNIAFSPPMYL*LL 424
EA HSGPRKSPA ARSWNWRSASIQRNP TLKRTAVS SSFEWSFMN+ FSPPMYL* L
Sbjct: 372 EAAHSGPRKSPASDARSWNWRSASIQRNPMTLKRTAVSLSSFEWSFMNMGFSPPMYL*SL 193
Query: 425 IDFSARKMLSTGPDLRNTPCLRILSTCPTMTSPFIGFWVMQVNETGNRTSPVSSRTFPSD 604
IDFS RKMLS GPDLR TPCLRI ST PT SPFIG VMQVN TG RTSPVSSRTFPS+
Sbjct: 192 IDFSDRKMLSIGPDLRKTPCLRIFSTEPTKISPFIGL*VMQVNVTGKRTSPVSSRTFPSE 13
Query: 605 IAFS 616
I FS
Sbjct: 12 IVFS 1
Score = 164 bits (352), Expect = 8e-040
Identities = 75/127 (59%), Positives = 88/127 (69%)
Frame = -3 / +1
Query: 616 AEGNI*RESA*ANWRCSVPSFIYLHYPEAYEGRGHGWARGQDPQAWRVPQIRTRGEHLPG 437
AE ++*RE +* + R S P I+LH+ EAYEG G RG+DPQA +PQIR EHLP
Sbjct: 1 AEDDL*REGS*TDRRRSFPGHIHLHHSEAYEGGDLGRLRGEDPQARGLPQIRANREHLPV 180
Query: 436 *EVDE*LQVHRWGECDVHERPLEAGEGHRRALQGPRVPLDGG*PPIPAPCYDRW*LPWAA 257
EVDE LQVH E VHE PL+AGEGHR ALQG VPLDGG P+PAPC W* P AA
Sbjct: 181 GEVDERLQVHGRREPHVHEGPLKAGEGHRCALQGHGVPLDGGRTPVPAPCVAGW*FPRAA 360
Query: 256 VNCFHRS 236
V+CF ++
Sbjct: 361 VSCFEQA 381
Score = 162 bits (349), Expect = 2e-039
Identities = 74/123 (60%), Positives = 89/123 (72%)
Frame = +1 / -3
Query: 247 SSSQRPKEVTSDRSKELELAVSLHPAEP*DLEAHGGVLLQLRVVVHEHRILPTYVLVITH 426
SSSQRP+E+TS R KELELA LHPAEP DLEAH GV LQL VV+HEH +L +VLV+ H
Sbjct: 370 SSSQRPEEITSQRRKELELAFCLHPAEPHDLEAHSGVPLQL*VVLHEHGVLAAHVLVVAH 191
Query: 427 RLLSQEDALHGSGFEEHAMLEDPVHVPNHDLSLHRLLGNASK*NWEQNISS*LTHFPFRY 606
RLL QEDAL GFEE +LED +H + DL LHR L +A + + E+N+S T+ PFR
Sbjct: 190 RLLRQEDALDWPGFEEDPVLEDLLHGADQDLPLHRPLSDAGECDREKNVSGQFTNLPFRD 11
Query: 607 CLQ 615
LQ
Sbjct: 10 RLQ 2
>gb|CB876258.1|CB876258 HX10M02w HX Hordeum vulgare subsp. vulgare cDNA clone HX10M02
3-PRIME, mRNA sequence
Length = 519
Score = 242 bits (522), Expect = 3e-063
Identities = 96/115 (83%), Positives = 111/115 (96%)
Frame = -1 / -1
Query: 588 RELTGDVLFPVSFTCITQKPMKGEVMVGHVDRILKHGVFLKSGPVESIFLAEKSMSNYKY 409
RELTGDVLFPV+FTCITQ+PMKGE++VG V++ILKHGVFLKSGP+ESIFL+EKSMS+YKY
Sbjct: 519 RELTGDVLFPVTFTCITQRPMKGEILVGSVEKILKHGVFLKSGPIESIFLSEKSMSDYKY 340
Query: 408 IGGENAMFMNDHSKLEKDTAVRFKVLGFRWMEADRQFQLLATIAGDFLGPL*TAS 244
+GGEN MFM DHSKLE+DTAVRFKV+GFRWMEA+RQFQLLA++AGDFLGPL* AS
Sbjct: 339 MGGENPMFMKDHSKLERDTAVRFKVMGFRWMEAERQFQLLASLAGDFLGPL*AAS 175
Score = 220 bits (475), Expect = 8e-057
Identities = 95/114 (83%), Positives = 96/114 (84%)
Frame = +2 / +2
Query: 245 EAVHSGPRKSPAIVARSWNWRSASIQRNPRTLKRTAVSFSSFEWSFMNIAFSPPMYL*LL 424
EA HSGPRKSPA ARSWNWRSASIQRNP TLKRTAVS SSFEWSFMN+ FSPPMYL* L
Sbjct: 176 EAAHSGPRKSPASDARSWNWRSASIQRNPMTLKRTAVSLSSFEWSFMNMGFSPPMYL*SL 355
Query: 425 IDFSARKMLSTGPDLRNTPCLRILSTCPTMTSPFIGFWVMQVNETGNRTSPVSS 586
IDFS RKMLS GPDLR TPCLRI ST PT SPFIG VMQVN TG RTSPVSS
Sbjct: 356 IDFSDRKMLSIGPDLRKTPCLRIFSTEPTKISPFIGL*VMQVNVTGKRTSPVSS 517
Score = 154 bits (330), Expect = 8e-037
Identities = 69/113 (61%), Positives = 78/113 (69%)
Frame = -3 / -3
Query: 574 RCSVPSFIYLHYPEAYEGRGHGWARGQDPQAWRVPQIRTRGEHLPG*EVDE*LQVHRWGE 395
R S P I+LH+ EAYEG G RG+DPQA +PQIR EHLP EVDE LQVH E
Sbjct: 505 RRSFPGHIHLHHSEAYEGGDLGRLRGEDPQARGLPQIRANREHLPVGEVDERLQVHGRRE 326
Query: 394 CDVHERPLEAGEGHRRALQGPRVPLDGG*PPIPAPCYDRW*LPWAAVNCFHRS 236
VHE PL+AGEGHR ALQG VPLDGG P+PAPC W* P AAV+CF ++
Sbjct: 325 PHVHEGPLKAGEGHRCALQGHGVPLDGGRTPVPAPCVAGW*FPRAAVSCFEQA 167
Score = 152 bits (327), Expect = 2e-036
Identities = 69/114 (60%), Positives = 83/114 (72%)
Frame = +1 / +1
Query: 247 SSSQRPKEVTSDRSKELELAVSLHPAEP*DLEAHGGVLLQLRVVVHEHRILPTYVLVITH 426
SSSQRP+E+TS R KELELA LHPAEP DLEAH GV LQL VV+HEH +L +VLV+ H
Sbjct: 178 SSSQRPEEITSQRRKELELAFCLHPAEPHDLEAHSGVPLQL*VVLHEHGVLAAHVLVVAH 357
Query: 427 RLLSQEDALHGSGFEEHAMLEDPVHVPNHDLSLHRLLGNASK*NWEQNISS*LT 588
RLL QEDAL GFEE +LED +H + DL LHR L +A + + E+N+S T
Sbjct: 358 RLLRQEDALDWPGFEEDPVLEDLLHGADQDLPLHRPLSDAGECDREKNVSGQFT 519
>gb|BM100842.2|BM100842 EBpi01_SQ002_C12_R pistil, 1 DPA, no treatment, cv Optic, EBpi01
Hordeum vulgare subsp. vulgare cDNA clone
EBpi01_SQ002_C12 5', mRNA sequence
Length = 467
Score = 188 bits (406), Expect = 3e-047
Identities = 75/92 (81%), Positives = 88/92 (95%)
Frame = -1 / +3
Query: 519 EVMVGHVDRILKHGVFLKSGPVESIFLAEKSMSNYKYIGGENAMFMNDHSKLEKDTAVRF 340
E++VG V++ILKHGVFLKSGP+ESIFL+EKSMS+YKY+GGEN MFM DHSKLE+DTAVRF
Sbjct: 3 EILVGSVEKILKHGVFLKSGPIESIFLSEKSMSDYKYMGGENPMFMKDHSKLERDTAVRF 182
Query: 339 KVLGFRWMEADRQFQLLATIAGDFLGPL*TAS 244
KV+GFRWMEA+RQFQLLA++AGDFLGPL* AS
Sbjct: 183 KVMGFRWMEAERQFQLLASLAGDFLGPL*AAS 278
Score = 181 bits (390), Expect = 4e-045
Identities = 77/92 (83%), Positives = 78/92 (84%)
Frame = +2 / -2
Query: 245 EAVHSGPRKSPAIVARSWNWRSASIQRNPRTLKRTAVSFSSFEWSFMNIAFSPPMYL*LL 424
EA HSGPRKSPA ARSWNWRSASIQRNP TLKRTAVS SSFEWSFMN+ FSPPMYL* L
Sbjct: 277 EAAHSGPRKSPASDARSWNWRSASIQRNPMTLKRTAVSLSSFEWSFMNMGFSPPMYL*SL 98
Query: 425 IDFSARKMLSTGPDLRNTPCLRILSTCPTMTS 520
IDFS RKMLS GPDLR TPCLRI ST PT S
Sbjct: 97 IDFSDRKMLSIGPDLRKTPCLRIFSTEPTKIS 2
Score = 135 bits (290), Expect = 3e-031
Identities = 60/91 (65%), Positives = 69/91 (75%)
Frame = +1 / -1
Query: 247 SSSQRPKEVTSDRSKELELAVSLHPAEP*DLEAHGGVLLQLRVVVHEHRILPTYVLVITH 426
SSSQRP+E+TS R KELELA LHPAEP DLEAH GV LQL VV+HEH +L +VLV+ H
Sbjct: 275 SSSQRPEEITSQRRKELELAFCLHPAEPHDLEAHSGVPLQL*VVLHEHGVLAAHVLVVAH 96
Query: 427 RLLSQEDALHGSGFEEHAMLEDPVHVPNHDL 519
RLL QEDAL GFEE +LED +H + DL
Sbjct: 95 RLLRQEDALDWPGFEEDPVLEDLLHGADQDL 3
Score = 130 bits (279), Expect = 9e-030
Identities = 58/92 (63%), Positives = 65/92 (70%)
Frame = -3 / +2
Query: 511 GWARGQDPQAWRVPQIRTRGEHLPG*EVDE*LQVHRWGECDVHERPLEAGEGHRRALQGP 332
G RG+DPQA +PQIR EHLP EVDE LQVH E VHE PL+AGEGHR ALQG
Sbjct: 11 GRLRGEDPQARGLPQIRANREHLPVGEVDERLQVHGRREPHVHEGPLKAGEGHRCALQGH 190
Query: 331 RVPLDGG*PPIPAPCYDRW*LPWAAVNCFHRS 236
VPLDGG P+PAPC W* P AAV+CF ++
Sbjct: 191 GVPLDGGRTPVPAPCVAGW*FPRAAVSCFEQA 286
>gb|BQ754697.1|BQ754697 EBed01_SQ003_I23_R endosperm, 6 DPA, no treatment, cv Optic, EBed01
Hordeum vulgare subsp. vulgare cDNA clone
EBed01_SQ003_I23 5', mRNA sequence
Length = 349
Score = 166 bits (356), Expect = 2e-040
Identities = 69/82 (84%), Positives = 76/82 (92%)
Frame = -1 / +2
Query: 780 MVFLEAEMSWNVLISPSQLDRKGLLLRKAIIVRLLEDVTHRRASKEHGYYIAVNQLKAIS 601
MVFL+ EMSWNVLISP QL KGLLLRK+IIVRLLED+T+R+AS EHGYYIAVN+LK IS
Sbjct: 104 MVFLQEEMSWNVLISPDQLSPKGLLLRKSIIVRLLEDITNRKASNEHGYYIAVNELKTIS 283
Query: 600 EGKVRELTGDVLFPVSFTCITQ 535
EGKVRELTGDVLFPV+FTCITQ
Sbjct: 284 EGKVRELTGDVLFPVTFTCITQ 349
Score = 114 bits (243), Expect = 8e-025
Identities = 55/81 (67%), Positives = 58/81 (71%)
Frame = +2 / -2
Query: 539 VMQVNETGNRTSPVSSRTFPSDIAFS*LTAM**PCSFEALLCVTSSRRRTMIAXXXXXXX 718
VMQVN TG RTSPVSSRTFPS+I FS LTAM**PCS EA L V SSRRRTMI
Sbjct: 345 VMQVNVTGKRTSPVSSRTFPSEIVFSSLTAM**PCSLEAFLLVMSSRRRTMIDLRSSRPL 166
Query: 719 XXXXXGEINTFHDISASRKTM 781
GEI+TF DIS+ RKTM
Sbjct: 165 GLSWSGEISTFQDISS*RKTM 103
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 431,187,186
Number of Sequences: 312970
Number of extensions: 7543728
Number of successful extensions: 59582
Number of sequences better than 1.0e-020: 8
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 10062793621
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)