TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521641.2.1
(594 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CK123410.1|CK123410 BES1824103p13 BES1824 Hordeum vulgar... 205 2e-052
gb|CA027804.1|CA027804 HZ60B10r HZ Hordeum vulgare subsp. v... 198 4e-051
gb|BQ665678.1|BQ665678 HX04K17u HX Hordeum vulgare subsp. v... 162 4e-042
gb|BJ467008.1|BJ467008 BJ467008 K. Sato unpublished cDNA li... 134 3e-031
gb|BU985192.1|BU985192 HF06G12r HF Hordeum vulgare subsp. v... 134 3e-031
gb|CK122715.1|CK122715 BES1824105i22 BES1824 Hordeum vulgar... 79 4e-024
gb|CK125884.1|CK125884 BES1824111A2a04 BES1824 Hordeum vulg... 79 4e-024
gb|CK123273.1|CK123273 BES1824103c09 BES1824 Hordeum vulgar... 79 4e-024
gb|BQ460303.1|BQ460303 HA06K13r HA Hordeum vulgare subsp. v... 79 4e-024
gb|BU984063.1|BU984063 HA31O02r HA Hordeum vulgare subsp. v... 79 4e-024
gb|CA023829.1|CA023829 HZ47I13r HZ Hordeum vulgare subsp. v... 110 6e-024
gb|CA019511.1|CA019511 HV12D10r HV Hordeum vulgare subsp. v... 67 1e-021
>gb|CK123410.1|CK123410 BES1824103p13 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P133 5-PRIME, mRNA sequence
Length = 579
Score = 205 bits (442), Expect = 2e-052
Identities = 80/98 (81%), Positives = 91/98 (92%)
Frame = -1 / +1
Query: 594 YMVGYGAKYPRRIHHRASSLPSVAAHPARIGCSQGFTALYAGGANPNLLVGAVVGGPDMQ 415
YMVGYGAKYP+++HHRASSLPSVAAHP +IGCSQGFT LY+G ANPN+ VGAVVGGP+
Sbjct: 121 YMVGYGAKYPKKLHHRASSLPSVAAHPGKIGCSQGFTGLYSGAANPNVHVGAVVGGPNQN 300
Query: 414 DRFPDERNDHEHSEPATYINAPLVGALAYLAHSYGQL* 301
D+FPD+R+D+EHSEPATYINAPLVGALAYLAHS GQL*
Sbjct: 301 DQFPDQRSDYEHSEPATYINAPLVGALAYLAHSSGQL* 414
Score = 144 bits (310), Expect = 3e-034
Identities = 68/97 (70%), Positives = 70/97 (72%)
Frame = +2 / -2
Query: 302 *SWP*ECAR*ASAPTSGALM*VAGSECSWSFRSSGNLSCMSGPPTTAPTSRLGLAPPAYS 481
*S P E AR*ASAPT GALM*VAGSECS S R SGN S GPPTTAPT GLA P
Sbjct: 413 *SXPEEWAR*ASAPTRGALM*VAGSECS*SLRWSGNWSFWFGPPTTAPTCTFGLAAPE*R 234
Query: 482 AVNPCEQPILAGCAATDGSDEARWWMRRGYLAP*PTM 592
VNPCE PI GCAAT+GS+EARW GYLAP PTM
Sbjct: 233 PVNPCEHPIFPGCAATEGSEEARWCSFFGYLAPYPTM 123
>gb|CA027804.1|CA027804 HZ60B10r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ60B10
5-PRIME, mRNA sequence
Length = 408
Score = 198 bits (426), Expect(2) = 4e-051
Identities = 77/95 (81%), Positives = 88/95 (92%)
Frame = -1 / +3
Query: 585 GYGAKYPRRIHHRASSLPSVAAHPARIGCSQGFTALYAGGANPNLLVGAVVGGPDMQDRF 406
GYGAKYP+++HHRASSLPSVAAHP +IGCSQGFT LY+G ANPN+ VGAVVGGP+ D+F
Sbjct: 12 GYGAKYPKKLHHRASSLPSVAAHPGKIGCSQGFTGLYSGAANPNVHVGAVVGGPNQNDQF 191
Query: 405 PDERNDHEHSEPATYINAPLVGALAYLAHSYGQL* 301
PD+R+D+EHSEPATYINAPLVGALAYLAHS GQL*
Sbjct: 192 PDQRSDYEHSEPATYINAPLVGALAYLAHSSGQL* 296
Score = 146 bits (313), Expect(2) = 7e-036
Identities = 67/95 (70%), Positives = 69/95 (72%)
Frame = +2 / -3
Query: 302 *SWP*ECAR*ASAPTSGALM*VAGSECSWSFRSSGNLSCMSGPPTTAPTSRLGLAPPAYS 481
*SWP E AR*ASAPT GALM*VAGSECS S R SGN S GPPTTAPT GLA P
Sbjct: 295 *SWPEEWAR*ASAPTRGALM*VAGSECS*SLRWSGNWSFWFGPPTTAPTCTFGLAAPE*R 116
Query: 482 AVNPCEQPILAGCAATDGSDEARWWMRRGYLAP*P 586
VNPCE PI GCAAT+GS+EARW GYLAP P
Sbjct: 115 PVNPCEHPIFPGCAATEGSEEARWCSFFGYLAPYP 11
Score = 24.0 bits (46), Expect(2) = 7e-036
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = +1 / -2
Query: 220 CLHWGRQIAML 252
CLHWG +IA +
Sbjct: 404 CLHWGLRIATI 372
Score = 23.1 bits (44), Expect(2) = 4e-051
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = -2 / +1
Query: 248 IAI*RPQCKQN 216
+AI RPQCKQ+
Sbjct: 376 VAIRRPQCKQH 408
>gb|BQ665678.1|BQ665678 HX04K17u HX Hordeum vulgare subsp. vulgare cDNA clone HX04K17
3-PRIME, mRNA sequence
Length = 517
Score = 162 bits (349), Expect(3) = 4e-042
Identities = 65/80 (81%), Positives = 73/80 (91%)
Frame = -1 / -3
Query: 540 SLPSVAAHPARIGCSQGFTALYAGGANPNLLVGAVVGGPDMQDRFPDERNDHEHSEPATY 361
SLPSVAAHP +IGCSQGFT LY+G ANPN+ VGAVVGGP+ D+FPD+R+D+EHSEPATY
Sbjct: 515 SLPSVAAHPGKIGCSQGFTGLYSGAANPNVHVGAVVGGPNQNDQFPDQRSDYEHSEPATY 336
Query: 360 INAPLVGALAYLAHSYGQL* 301
INAPLVGALAYLAHS GQL*
Sbjct: 335 INAPLVGALAYLAHSSGQL* 276
Score = 124 bits (266), Expect(2) = 2e-029
Identities = 57/80 (71%), Positives = 59/80 (73%)
Frame = +2 / +1
Query: 302 *SWP*ECAR*ASAPTSGALM*VAGSECSWSFRSSGNLSCMSGPPTTAPTSRLGLAPPAYS 481
*SWP E AR*ASAPT GALM*VAGSECS S R SGN S GPPTTAPT GLA P
Sbjct: 277 *SWPEEWAR*ASAPTRGALM*VAGSECS*SLRWSGNWSFWFGPPTTAPTCTFGLAAPE*R 456
Query: 482 AVNPCEQPILAGCAATDGSD 541
VNPCE PI GCAAT+GS+
Sbjct: 457 PVNPCEHPIFPGCAATEGSE 516
Score = 24.0 bits (46), Expect(2) = 2e-029
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = +1 / +3
Query: 220 CLHWGRQIAML 252
CLHWG +IA +
Sbjct: 168 CLHWGLRIATI 200
Score = 23.5 bits (45), Expect(3) = 4e-042
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = -3 / -1
Query: 88 TKCTATKVAARNSI* 44
T CT VAARNS+*
Sbjct: 67 TTCTQVVVAARNSL* 23
Score = 23.1 bits (44), Expect(3) = 4e-042
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = -2 / -1
Query: 248 IAI*RPQCKQN 216
+AI RPQCKQ+
Sbjct: 196 VAIRRPQCKQH 164
>gb|BJ467008.1|BJ467008 BJ467008 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags35i04 3', mRNA sequence
Length = 786
Score = 134 bits (288), Expect = 3e-031
Identities = 53/89 (59%), Positives = 62/89 (69%)
Frame = -1 / -3
Query: 594 YMVGYGAKYPRRIHHRASSLPSVAAHPARIGCSQGFTALYAGGANPNLLVGAVVGGPDMQ 415
YMVGYG +YP+RIHHR SSLPS+ HP R+ C G + NPN LVGA+VGGP
Sbjct: 679 YMVGYGDRYPQRIHHRGSSLPSIKDHPERMACKDGTPYYDSSSPNPNPLVGAIVGGPGED 500
Query: 414 DRFPDERNDHEHSEPATYINAPLVGALAY 328
D + D+R D+ SEP TYINAPLVG LAY
Sbjct: 499 DSYGDDRADYRKSEPTTYINAPLVGVLAY 413
>gb|BU985192.1|BU985192 HF06G12r HF Hordeum vulgare subsp. vulgare cDNA clone HF06G12
5-PRIME, mRNA sequence
Length = 615
Score = 134 bits (288), Expect = 3e-031
Identities = 53/89 (59%), Positives = 62/89 (69%)
Frame = -1 / +3
Query: 594 YMVGYGAKYPRRIHHRASSLPSVAAHPARIGCSQGFTALYAGGANPNLLVGAVVGGPDMQ 415
YMVGYG +YP+RIHHR SSLPS+ HP R+ C G + NPN LVGA+VGGP
Sbjct: 177 YMVGYGDRYPQRIHHRGSSLPSIKDHPERMACKDGTPYYDSSSPNPNPLVGAIVGGPGED 356
Query: 414 DRFPDERNDHEHSEPATYINAPLVGALAY 328
D + D+R D+ SEP TYINAPLVG LAY
Sbjct: 357 DSYGDDRADYRKSEPTTYINAPLVGVLAY 443
>gb|CK122715.1|CK122715 BES1824105i22 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010I225 5-PRIME, mRNA sequence
Length = 776
Score = 79.0 bits (166), Expect(2) = 4e-024
Identities = 32/53 (60%), Positives = 38/53 (71%)
Frame = -1 / +2
Query: 468 GANPNLLVGAVVGGPDMQDRFPDERNDHEHSEPATYINAPLVGALAYLAHSYG 310
G+NPNLL GA+VGGPD D F DERN++E +E TY NAP +G LA LA G
Sbjct: 236 GSNPNLLDGAIVGGPDEYDNFADERNNYEQTEATTYNNAPFMGVLARLAAGQG 394
Score = 51.9 bits (107), Expect(2) = 4e-024
Identities = 19/40 (47%), Positives = 25/40 (62%)
Frame = -1 / +2
Query: 594 YMVGYGAKYPRRIHHRASSLPSVAAHPARIGCSQGFTALY 475
YMVGYG YP HHR +S+ SV ++PA + C G+ Y
Sbjct: 107 YMVGYGFIYPLEAHHRGASIVSVKSNPAFVSCRGGYATWY 226
>gb|CK125884.1|CK125884 BES1824111A2a04 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010A0411 5-PRIME, mRNA sequence
Length = 748
Score = 79.0 bits (166), Expect(2) = 4e-024
Identities = 32/53 (60%), Positives = 38/53 (71%)
Frame = -1 / +2
Query: 468 GANPNLLVGAVVGGPDMQDRFPDERNDHEHSEPATYINAPLVGALAYLAHSYG 310
G+NPNLL GA+VGGPD D F DERN++E +E TY NAP +G LA LA G
Sbjct: 422 GSNPNLLDGAIVGGPDEYDNFADERNNYEQTEATTYNNAPFMGVLARLAAGQG 580
Score = 51.9 bits (107), Expect(2) = 4e-024
Identities = 19/40 (47%), Positives = 25/40 (62%)
Frame = -1 / +2
Query: 594 YMVGYGAKYPRRIHHRASSLPSVAAHPARIGCSQGFTALY 475
YMVGYG YP HHR +S+ SV ++PA + C G+ Y
Sbjct: 293 YMVGYGFIYPLEAHHRGASIVSVKSNPAFVSCRGGYATWY 412
>gb|CK123273.1|CK123273 BES1824103c09 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010C093 5-PRIME, mRNA sequence
Length = 594
Score = 79.0 bits (166), Expect(2) = 4e-024
Identities = 32/53 (60%), Positives = 38/53 (71%)
Frame = -1 / +2
Query: 468 GANPNLLVGAVVGGPDMQDRFPDERNDHEHSEPATYINAPLVGALAYLAHSYG 310
G+NPNLL GA+VGGPD D F DERN++E +E TY NAP +G LA LA G
Sbjct: 293 GSNPNLLDGAIVGGPDEYDNFADERNNYEQTEATTYNNAPFMGVLARLAAGQG 451
Score = 51.9 bits (107), Expect(2) = 4e-024
Identities = 19/40 (47%), Positives = 25/40 (62%)
Frame = -1 / +2
Query: 594 YMVGYGAKYPRRIHHRASSLPSVAAHPARIGCSQGFTALY 475
YMVGYG YP HHR +S+ SV ++PA + C G+ Y
Sbjct: 164 YMVGYGFIYPLEAHHRGASIVSVKSNPAFVSCRGGYATWY 283
>gb|BQ460303.1|BQ460303 HA06K13r HA Hordeum vulgare subsp. vulgare cDNA clone HA06K13
5-PRIME, mRNA sequence
Length = 581
Score = 79.0 bits (166), Expect(2) = 4e-024
Identities = 32/53 (60%), Positives = 38/53 (71%)
Frame = -1 / +1
Query: 468 GANPNLLVGAVVGGPDMQDRFPDERNDHEHSEPATYINAPLVGALAYLAHSYG 310
G+NPNLL GA+VGGPD D F DERN++E +E TY NAP +G LA LA G
Sbjct: 205 GSNPNLLDGAIVGGPDEYDNFADERNNYEQTEATTYNNAPFMGVLARLAAGQG 363
Score = 51.9 bits (107), Expect(2) = 4e-024
Identities = 19/40 (47%), Positives = 25/40 (62%)
Frame = -1 / +1
Query: 594 YMVGYGAKYPRRIHHRASSLPSVAAHPARIGCSQGFTALY 475
YMVGYG YP HHR +S+ SV ++PA + C G+ Y
Sbjct: 76 YMVGYGFIYPLEAHHRGASIVSVKSNPAFVSCRGGYATWY 195
>gb|BU984063.1|BU984063 HA31O02r HA Hordeum vulgare subsp. vulgare cDNA clone HA31O02
5-PRIME, mRNA sequence
Length = 477
Score = 79.0 bits (166), Expect(2) = 4e-024
Identities = 32/53 (60%), Positives = 38/53 (71%)
Frame = -1 / +3
Query: 468 GANPNLLVGAVVGGPDMQDRFPDERNDHEHSEPATYINAPLVGALAYLAHSYG 310
G+NPNLL GA+VGGPD D F DERN++E +E TY NAP +G LA LA G
Sbjct: 162 GSNPNLLDGAIVGGPDEYDNFADERNNYEQTEATTYNNAPFMGVLARLAAGQG 320
Score = 51.9 bits (107), Expect(2) = 4e-024
Identities = 19/40 (47%), Positives = 25/40 (62%)
Frame = -1 / +3
Query: 594 YMVGYGAKYPRRIHHRASSLPSVAAHPARIGCSQGFTALY 475
YMVGYG YP HHR +S+ SV ++PA + C G+ Y
Sbjct: 33 YMVGYGFIYPLEAHHRGASIVSVKSNPAFVSCRGGYATWY 152
>gb|CA023829.1|CA023829 HZ47I13r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ47I13
5-PRIME, mRNA sequence
Length = 460
Score = 110 bits (235), Expect = 6e-024
Identities = 41/51 (80%), Positives = 47/51 (92%)
Frame = -1 / +2
Query: 594 YMVGYGAKYPRRIHHRASSLPSVAAHPARIGCSQGFTALYAGGANPNLLVG 442
YMVGYGAKYP+++HHRASSLPSVAAHP +IGCSQGFT LY+G ANPN+ VG
Sbjct: 308 YMVGYGAKYPKKLHHRASSLPSVAAHPGKIGCSQGFTGLYSGAANPNVHVG 460
>gb|CA019511.1|CA019511 HV12D10r HV Hordeum vulgare subsp. vulgare cDNA clone HV12D10
5-PRIME, mRNA sequence
Length = 480
Score = 66.6 bits (139), Expect(2) = 1e-021
Identities = 26/47 (55%), Positives = 34/47 (72%)
Frame = -1 / +3
Query: 465 ANPNLLVGAVVGGPDMQDRFPDERNDHEHSEPATYINAPLVGALAYL 325
ANPN++VGA+VGGPD DRF D+R ++ +E TY AP+VG A L
Sbjct: 297 ANPNVVVGAIVGGPDRHDRFRDDRVNYMQTEACTYNTAPMVGMFAML 437
Score = 55.6 bits (115), Expect(2) = 1e-021
Identities = 20/36 (55%), Positives = 26/36 (72%)
Frame = -1 / +3
Query: 594 YMVGYGAKYPRRIHHRASSLPSVAAHPARIGCSQGF 487
Y+VGYGAK+P R+HHRA+S+ IGC+QGF
Sbjct: 165 YLVGYGAKFPARVHHRAASIVPYKDRKEFIGCAQGF 272
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 288,864,527
Number of Sequences: 312970
Number of extensions: 5129846
Number of successful extensions: 126886
Number of sequences better than 1.0e-020: 16
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6192839514
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)