TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493961.2.1
(733 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV064016.1|CV064016 BNEL96d6 Barley EST endosperm librar... 112 2e-049
gb|CV061470.1|CV061470 BNEL69c12 Barley EST endosperm libra... 126 1e-044
gb|BI949354.1|BI949354 HVSMEl0013I01f Hordeum vulgare spike... 124 5e-044
gb|CV054964.1|CV054964 BNEL115c6 Barley EST endosperm libra... 126 6e-042
gb|BQ460467.1|BQ460467 HA09H21r HA Hordeum vulgare subsp. v... 168 4e-041
gb|BU983843.1|BU983843 HA31D12r HA Hordeum vulgare subsp. v... 168 4e-041
gb|BQ657142.1|BQ657142 HA09H21u HA Hordeum vulgare subsp. v... 162 2e-039
gb|CV055699.1|CV055699 BNEL122e2 Barley EST endosperm libra... 95 4e-035
gb|BQ756160.1|BQ756160 EBem05_SQ001_P18_R embryo, 14 DPA, n... 142 2e-033
gb|CV053723.1|CV053723 BNEL102e12 Barley EST endosperm libr... 142 2e-033
gb|CV061337.1|CV061337 BNEL67f6 Barley EST endosperm librar... 142 2e-033
gb|CV062016.1|CV062016 BNEL75d5 Barley EST endosperm librar... 142 2e-033
gb|CV062510.1|CV062510 BNEL7H1 Barley EST endosperm library... 142 2e-033
gb|CV062698.1|CV062698 BNEL82a1 Barley EST endosperm librar... 142 2e-033
gb|CV063833.1|CV063833 BNEL93f8 Barley EST endosperm librar... 142 2e-033
gb|CV058935.1|CV058935 BNEL42f10 Barley EST endosperm libra... 125 3e-028
gb|CV057514.1|CV057514 BNEL28g10 Barley EST endosperm libra... 105 5e-025
gb|BJ467007.1|BJ467007 BJ467007 K. Sato unpublished cDNA li... 64 3e-023
gb|CV058244.1|CV058244 BNEL35h4 Barley EST endosperm librar... 105 2e-022
gb|CV058639.1|CV058639 BNEL3C10 Barley EST endosperm librar... 104 7e-022
>gb|CV064016.1|CV064016 BNEL96d6 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL96d6 5' similar to putative alpha
galactosyltransferase, mRNA sequence
Length = 746
Score = 112 bits (239), Expect(2) = 2e-049
Identities = 43/63 (68%), Positives = 46/63 (73%)
Frame = -2 / +2
Query: 396 ETGVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASS 217
E+GV GWRRP VTHF GCQPCSG RN YSGDSCD G+RRALN D+QVL FRHA
Sbjct: 557 ESGVKGWRRPVVTHFNGCQPCSGDRNPDYSGDSCDDGIRRALNXGDEQVLXDLRFRHAGR 736
Query: 216 LSD 208
D
Sbjct: 737 SXD 745
Score = 105 bits (225), Expect(2) = 5e-043
Identities = 44/59 (74%), Positives = 47/59 (79%)
Frame = +1 / -2
Query: 475 QRGSSRPPLHRLVPLRHVGEPPHDLHPVALEVVAALQEHLVPPPAALLQQHVHERGLVV 651
QR RPPLHRLVP R EPPHDLHPVALEVVA LQEHL+PP AA + QHVH+R LVV
Sbjct: 472 QRWRRRPPLHRLVPRRDAAEPPHDLHPVALEVVAVLQEHLLPPRAAPVLQHVHQRRLVV 296
Score = 103 bits (220), Expect(2) = 2e-049
Identities = 40/53 (75%), Positives = 45/53 (84%)
Frame = -2 / +2
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERYEAMERR 493
DDQSALVYML GS WR+KVFLE+GYYF+GYW+EIVGRL + RYEAMERR
Sbjct: 296 DDQSALVYMLQHRGSPWREKVFLENGYYFQGYWVEIVGRLGGIAARYEAMERR 454
Score = 90.4 bits (191), Expect(2) = 9e-022
Identities = 41/53 (77%), Positives = 42/53 (79%)
Frame = +2 / -3
Query: 494 RRSIASYLSVMLASRPTISIQ*PSK**PLSRNTLSRHRLPSCSSMYTSADWSS 652
RRSIASY + M SRPTIS Q*P K**P SRNT SRH LP C SMYTSADWSS
Sbjct: 453 RRSIASYRAAMPPSRPTISTQ*PWK**PFSRNTFSRHGLPLCCSMYTSADWSS 295
Score = 88.6 bits (187), Expect(2) = 5e-043
Identities = 41/73 (56%), Positives = 43/73 (58%)
Frame = +2 / -3
Query: 224 ACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLHG*HPVKCVTNGRRHPCTXXXXX 403
AC + ST SS KLSARRMP SQLSPE* R PLHG HP+KCVT GR HP T
Sbjct: 729 ACRNRKSXSTCSSPKLSARRMPSSQLSPE*SGFRSPLHGWHPLKCVTTGRLHPLTPLSAS 550
Query: 404 XXXXXXXFLATAY 442
LA AY
Sbjct: 549 PAPASAASLACAY 511
Score = 32.7 bits (65), Expect(2) = 9e-022
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +1 / -2
Query: 334 ARLTPREVRHERAAPPVHPGLRQPGPAQGGVP 429
A L P EVRH+ PP+ LR+P Q +P
Sbjct: 619 AWLAPVEVRHDGPPPPLDAALREPRARQRRLP 524
>gb|CV061470.1|CV061470 BNEL69c12 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL69c12 5' similar to Oryza sativa,
mRNA sequence
Length = 589
Score = 126 bits (269), Expect(2) = 1e-044
Identities = 48/64 (75%), Positives = 52/64 (81%)
Frame = -2 / -3
Query: 378 WRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASSLSDDVQ 199
WRRPFVTHFTGC PC G+ NE YS ++C GMRRALN ADDQVLRAYGFRHA L DDV+
Sbjct: 296 WRRPFVTHFTGCNPCGGKPNEIYSNETCADGMRRALNLADDQVLRAYGFRHAGPLKDDVR 117
Query: 198 PLPF 187
PL F
Sbjct: 116 PL*F 105
Score = 87.2 bits (184), Expect(2) = 2e-026
Identities = 43/63 (68%), Positives = 46/63 (73%)
Frame = +2 / +1
Query: 188 NGSGCTSSLSELACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLHG*HPVKCVTN 367
N SG TSSLS A KP A STWSSA+LSARRMP +Q+S E* S P G HPVKCVTN
Sbjct: 106 N*SGRTSSLSGPAWRKP*ARSTWSSARLSARRMPSAQVSLE*ISFGLPPQGLHPVKCVTN 285
Query: 368 GRR 376
GRR
Sbjct: 286 GRR 294
Score = 75.8 bits (159), Expect(2) = 1e-044
Identities = 29/55 (52%), Positives = 37/55 (67%)
Frame = -2 / -3
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERYEAMERRPG 487
DDQSAL Y+LL+ +W + +LE YYF+GYW EIV +L + RY A ERR G
Sbjct: 584 DDQSALAYLLLKNRKKWGARTYLEHDYYFQGYWAEIVDKLDGVAARYRAAERRFG 420
Score = 53.3 bits (110), Expect(2) = 2e-026
Identities = 29/55 (52%), Positives = 32/55 (58%)
Frame = +2 / +1
Query: 488 PGRRSIASYLSVMLASRPTISIQ*PSK**PLSRNTLSRHRLPSCSSMYTSADWSS 652
P RRS A YL+ +S TIS Q P K** SR L+ H L S Y SADWSS
Sbjct: 421 PNRRSAALYLAATPSSLSTISAQYPWK**SCSRYVLAPHFLRFLSRRYASADWSS 585
>gb|BI949354.1|BI949354 HVSMEl0013I01f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0013I01f, mRNA sequence
Length = 950
Score = 124 bits (266), Expect(2) = 5e-044
Identities = 47/64 (73%), Positives = 52/64 (81%)
Frame = -2 / +3
Query: 378 WRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASSLSDDVQ 199
WRRPFVTHFTGC PC G+ NE YS ++C GMRRALN ADDQVLRAYGFRHA L DD++
Sbjct: 369 WRRPFVTHFTGCNPCGGKPNEIYSNETCADGMRRALNLADDQVLRAYGFRHAGPLKDDLR 548
Query: 198 PLPF 187
PL F
Sbjct: 549 PL*F 560
Score = 84.5 bits (178), Expect(2) = 5e-026
Identities = 42/63 (66%), Positives = 46/63 (73%)
Frame = +2 / -2
Query: 188 NGSGCTSSLSELACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLHG*HPVKCVTN 367
N SG SSLS A KP A STWSSA+LSARR+P +Q+S E* SL P G HPVKCVTN
Sbjct: 559 N*SGRKSSLSGPAWRKP*ARSTWSSARLSARRIPSAQVSLE*ISLGLPPQGLHPVKCVTN 380
Query: 368 GRR 376
GRR
Sbjct: 379 GRR 371
Score = 75.8 bits (159), Expect(2) = 5e-044
Identities = 29/55 (52%), Positives = 37/55 (67%)
Frame = -2 / +3
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERYEAMERRPG 487
DDQSAL Y+LL+ +W + +LE YYF+GYW EIV +L + RY A ERR G
Sbjct: 81 DDQSALAYLLLKNRKKWGARTYLEHDYYFQGYWAEIVDKLDGVAARYRAAERRFG 245
Score = 55.6 bits (115), Expect(2) = 5e-026
Identities = 30/55 (54%), Positives = 33/55 (60%)
Frame = +2 / -2
Query: 488 PGRRSIASYLSVMLASRPTISIQ*PSK**PLSRNTLSRHRLPSCSSMYTSADWSS 652
P RRS A YL+ +S TIS Q P K** SR L+ H L SS Y SADWSS
Sbjct: 244 PNRRSAALYLAATPSSLSTISAQYPWK**SCSRYVLAPHFLRFLSSRYASADWSS 80
>gb|CV054964.1|CV054964 BNEL115c6 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL115c6 5' similar to Oryza sativa,
mRNA sequence
Length = 593
Score = 126 bits (269), Expect(2) = 6e-042
Identities = 48/64 (75%), Positives = 52/64 (81%)
Frame = -2 / -1
Query: 378 WRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASSLSDDVQ 199
WRRPFVTHFTGC PC G+ NE YS ++C GMRRALN ADDQVLRAYGFRHA L DDV+
Sbjct: 314 WRRPFVTHFTGCNPCGGKPNEIYSNETCADGMRRALNLADDQVLRAYGFRHAGPLKDDVR 135
Query: 198 PLPF 187
PL F
Sbjct: 134 PL*F 123
Score = 87.2 bits (184), Expect(2) = 3e-025
Identities = 43/63 (68%), Positives = 46/63 (73%)
Frame = +2 / +1
Query: 188 NGSGCTSSLSELACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLHG*HPVKCVTN 367
N SG TSSLS A KP A STWSSA+LSARRMP +Q+S E* S P G HPVKCVTN
Sbjct: 124 N*SGRTSSLSGPAWRKP*ARSTWSSARLSARRMPSAQVSLE*ISFGLPPQGLHPVKCVTN 303
Query: 368 GRR 376
GRR
Sbjct: 304 GRR 312
Score = 66.6 bits (139), Expect(2) = 6e-042
Identities = 25/52 (48%), Positives = 34/52 (65%)
Frame = -2 / -1
Query: 642 SALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERYEAMERRPG 487
SAL Y+LL+ +W + +LE YYF+GYW EIV +L + RY + ERR G
Sbjct: 593 SALAYLLLKNRXKWGARTYLEHDYYFQGYWAEIVDKLDGVAARYRSAERRFG 438
Score = 49.6 bits (102), Expect(2) = 3e-025
Identities = 25/49 (51%), Positives = 29/49 (59%)
Frame = +1 / +3
Query: 493 PPLHRLVPLRHVGEPPHDLHPVALEVVAALQEHLVPPPAALLQQHVHER 639
PPL R VP R E HDL PV+LEVV LQ P A+L+Q V +R
Sbjct: 444 PPLCRPVPRRDAVELVHDLRPVSLEVVVVLQVRPGAPLXAVLEQQVCQR 590
>gb|BQ460467.1|BQ460467 HA09H21r HA Hordeum vulgare subsp. vulgare cDNA clone HA09H21
5-PRIME, mRNA sequence
Length = 388
Score = 168 bits (361), Expect = 4e-041
Identities = 64/76 (84%), Positives = 67/76 (88%)
Frame = -2 / +2
Query: 396 ETGVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASS 217
E+GV GWRRPFVTHFTGCQPCSG RN YSGDSCD G+RRALNFADDQVLR YGFRHA
Sbjct: 80 ESGVKGWRRPFVTHFTGCQPCSGDRNRDYSGDSCDDGIRRALNFADDQVLRDYGFRHAGP 259
Query: 216 LSDDVQPLPFDYPAKA 169
LSDDV+PLPFDYPA A
Sbjct: 260 LSDDVRPLPFDYPAAA 307
Score = 131 bits (280), Expect = 5e-030
Identities = 62/95 (65%), Positives = 64/95 (67%)
Frame = +2 / -2
Query: 158 QLVTAFAG*SNGSGCTSSLSELACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLH 337
Q + A AG*SNGSG TSSLS AC P +LSTWSSAKLSARRMP SQLSPE* R PLH
Sbjct: 318 QRLPAAAG*SNGSGRTSSLSGPACRNP*SLSTWSSAKLSARRMPSSQLSPE*SRFRSPLH 139
Query: 338 G*HPVKCVTNGRRHPCTXXXXXXXXXXXXFLATAY 442
G HPVKCVTNGR HP T LA AY
Sbjct: 138 GWHPVKCVTNGRLHPLTPLSASPAPASAASLACAY 34
>gb|BU983843.1|BU983843 HA31D12r HA Hordeum vulgare subsp. vulgare cDNA clone HA31D12
5-PRIME, mRNA sequence
Length = 354
Score = 168 bits (361), Expect = 4e-041
Identities = 64/76 (84%), Positives = 67/76 (88%)
Frame = -2 / +3
Query: 396 ETGVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASS 217
E+GV GWRRPFVTHFTGCQPCSG RN YSGDSCD G+RRALNFADDQVLR YGFRHA
Sbjct: 78 ESGVKGWRRPFVTHFTGCQPCSGDRNRDYSGDSCDDGIRRALNFADDQVLRDYGFRHAGP 257
Query: 216 LSDDVQPLPFDYPAKA 169
LSDDV+PLPFDYPA A
Sbjct: 258 LSDDVRPLPFDYPAAA 305
Score = 129 bits (277), Expect = 1e-029
Identities = 61/95 (64%), Positives = 64/95 (67%)
Frame = +2 / -3
Query: 158 QLVTAFAG*SNGSGCTSSLSELACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLH 337
Q + A AG*SNG+G TSSLS AC P +LSTWSSAKLSARRMP SQLSPE* R PLH
Sbjct: 316 QRLPAAAG*SNGNGRTSSLSGPACRNP*SLSTWSSAKLSARRMPSSQLSPE*SRFRSPLH 137
Query: 338 G*HPVKCVTNGRRHPCTXXXXXXXXXXXXFLATAY 442
G HPVKCVTNGR HP T LA AY
Sbjct: 136 GWHPVKCVTNGRLHPLTPLSASPAPASAASLACAY 32
>gb|BQ657142.1|BQ657142 HA09H21u HA Hordeum vulgare subsp. vulgare cDNA clone HA09H21
3-PRIME, mRNA sequence
Length = 401
Score = 162 bits (349), Expect = 2e-039
Identities = 62/73 (84%), Positives = 64/73 (87%)
Frame = -2 / -3
Query: 387 VHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASSLSD 208
V GWRRPFVTHFTGCQPCSG RN YSGDSCD G+RRALNFADDQVLR YGFRHA LSD
Sbjct: 399 VKGWRRPFVTHFTGCQPCSGDRNRDYSGDSCDDGIRRALNFADDQVLRDYGFRHAGPLSD 220
Query: 207 DVQPLPFDYPAKA 169
DV+PLPFDYPA A
Sbjct: 219 DVRPLPFDYPAAA 181
Score = 127 bits (271), Expect = 9e-029
Identities = 58/77 (75%), Positives = 60/77 (77%)
Frame = +2 / +2
Query: 158 QLVTAFAG*SNGSGCTSSLSELACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLH 337
Q + A AG*SNGSG TSSLS AC P +LSTWSSAKLSARRMP SQLSPE* R PLH
Sbjct: 170 QRLPAAAG*SNGSGRTSSLSGPACRNP*SLSTWSSAKLSARRMPSSQLSPE*SRFRSPLH 349
Query: 338 G*HPVKCVTNGRRHPCT 388
G HPVKCVTNGR HP T
Sbjct: 350 GWHPVKCVTNGRLHPLT 400
>gb|CV055699.1|CV055699 BNEL122e2 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL122e2 5' similar to alpha
galactosyltransferase, mRNA sequence
Length = 808
Score = 94.5 bits (200), Expect(2) = 4e-035
Identities = 36/54 (66%), Positives = 40/54 (74%)
Frame = -2 / +3
Query: 378 WRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASS 217
WRRPFVTHFTGC PC G+ NE + + MRRALN DDQVLRAYGFRHA +
Sbjct: 414 WRRPFVTHFTGCNPCGGKANEILLKQTLAERMRRALNLGDDQVLRAYGFRHAGT 575
Score = 75.8 bits (159), Expect(2) = 4e-035
Identities = 29/55 (52%), Positives = 37/55 (67%)
Frame = -2 / +3
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERYEAMERRPG 487
DDQSAL Y+LL+ +W + +LE YYF+GYW EIV +L + RY A ERR G
Sbjct: 126 DDQSALAYLLLKNRKKWGARTYLEHDYYFQGYWAEIVDKLDGVAARYRAAERRFG 290
>gb|BQ756160.1|BQ756160 EBem05_SQ001_P18_R embryo, 14 DPA, no treatment, cv Optic, EBem05
Hordeum vulgare subsp. vulgare cDNA clone
EBem05_SQ001_P18 5', mRNA sequence
Length = 246
Score = 142 bits (305), Expect = 2e-033
Identities = 54/64 (84%), Positives = 56/64 (87%)
Frame = -2 / +2
Query: 396 ETGVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASS 217
E+GV GWRRPFVTHFTGCQPCSG RN YSGDSCD G+RRALNFADDQVLR YGFRHA
Sbjct: 53 ESGVKGWRRPFVTHFTGCQPCSGDRNRDYSGDSCDDGIRRALNFADDQVLRDYGFRHAGP 232
Query: 216 LSDD 205
LSDD
Sbjct: 233 LSDD 244
Score = 113 bits (241), Expect = 1e-024
Identities = 52/80 (65%), Positives = 53/80 (66%)
Frame = +2 / -1
Query: 203 TSSLSELACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLHG*HPVKCVTNGRRHP 382
TSSLS AC P +LSTWSSAKLSARRMP SQLSPE* R PLHG HPVKCVTNGR HP
Sbjct: 246 TSSLSGPACRNP*SLSTWSSAKLSARRMPSSQLSPE*SRFRSPLHGWHPVKCVTNGRLHP 67
Query: 383 CTXXXXXXXXXXXXFLATAY 442
T LA AY
Sbjct: 66 LTPLSASPAPASAASLACAY 7
>gb|CV053723.1|CV053723 BNEL102e12 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL102e12 5' similar to putative
alpha galactosyltransferase, mRNA sequence
Length = 298
Score = 142 bits (305), Expect = 2e-033
Identities = 54/64 (84%), Positives = 56/64 (87%)
Frame = -2 / +3
Query: 396 ETGVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASS 217
E+GV GWRRPFVTHFTGCQPCSG RN YSGDSCD G+RRALNFADDQVLR YGFRHA
Sbjct: 105 ESGVKGWRRPFVTHFTGCQPCSGDRNRDYSGDSCDDGIRRALNFADDQVLRDYGFRHAGP 284
Query: 216 LSDD 205
LSDD
Sbjct: 285 LSDD 296
Score = 113 bits (241), Expect = 1e-024
Identities = 52/80 (65%), Positives = 53/80 (66%)
Frame = +2 / -1
Query: 203 TSSLSELACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLHG*HPVKCVTNGRRHP 382
TSSLS AC P +LSTWSSAKLSARRMP SQLSPE* R PLHG HPVKCVTNGR HP
Sbjct: 298 TSSLSGPACRNP*SLSTWSSAKLSARRMPSSQLSPE*SRFRSPLHGWHPVKCVTNGRLHP 119
Query: 383 CTXXXXXXXXXXXXFLATAY 442
T LA AY
Sbjct: 118 LTPLSASPAPASAASLACAY 59
>gb|CV061337.1|CV061337 BNEL67f6 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL67f6 5' similar to putative alpha
galactosyltransferase, mRNA sequence
Length = 298
Score = 142 bits (305), Expect = 2e-033
Identities = 54/64 (84%), Positives = 56/64 (87%)
Frame = -2 / +3
Query: 396 ETGVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASS 217
E+GV GWRRPFVTHFTGCQPCSG RN YSGDSCD G+RRALNFADDQVLR YGFRHA
Sbjct: 105 ESGVKGWRRPFVTHFTGCQPCSGDRNRDYSGDSCDDGIRRALNFADDQVLRDYGFRHAGP 284
Query: 216 LSDD 205
LSDD
Sbjct: 285 LSDD 296
Score = 113 bits (241), Expect = 1e-024
Identities = 52/80 (65%), Positives = 53/80 (66%)
Frame = +2 / -1
Query: 203 TSSLSELACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLHG*HPVKCVTNGRRHP 382
TSSLS AC P +LSTWSSAKLSARRMP SQLSPE* R PLHG HPVKCVTNGR HP
Sbjct: 298 TSSLSGPACRNP*SLSTWSSAKLSARRMPSSQLSPE*SRFRSPLHGWHPVKCVTNGRLHP 119
Query: 383 CTXXXXXXXXXXXXFLATAY 442
T LA AY
Sbjct: 118 LTPLSASPAPASAASLACAY 59
>gb|CV062016.1|CV062016 BNEL75d5 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL75d5 5' similar to putative alpha
galactosyltransferase, mRNA sequence
Length = 298
Score = 142 bits (305), Expect = 2e-033
Identities = 54/64 (84%), Positives = 56/64 (87%)
Frame = -2 / +3
Query: 396 ETGVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASS 217
E+GV GWRRPFVTHFTGCQPCSG RN YSGDSCD G+RRALNFADDQVLR YGFRHA
Sbjct: 105 ESGVKGWRRPFVTHFTGCQPCSGDRNRDYSGDSCDDGIRRALNFADDQVLRDYGFRHAGP 284
Query: 216 LSDD 205
LSDD
Sbjct: 285 LSDD 296
Score = 113 bits (241), Expect = 1e-024
Identities = 52/80 (65%), Positives = 53/80 (66%)
Frame = +2 / -1
Query: 203 TSSLSELACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLHG*HPVKCVTNGRRHP 382
TSSLS AC P +LSTWSSAKLSARRMP SQLSPE* R PLHG HPVKCVTNGR HP
Sbjct: 298 TSSLSGPACRNP*SLSTWSSAKLSARRMPSSQLSPE*SRFRSPLHGWHPVKCVTNGRLHP 119
Query: 383 CTXXXXXXXXXXXXFLATAY 442
T LA AY
Sbjct: 118 LTPLSASPAPASAASLACAY 59
>gb|CV062510.1|CV062510 BNEL7H1 Barley EST endosperm library Hordeum vulgare subsp. vulgare
cDNA clone BNEL7H1 5' similar to putative alpha
galactosyltransferase, mRNA sequence
Length = 298
Score = 142 bits (305), Expect = 2e-033
Identities = 54/64 (84%), Positives = 56/64 (87%)
Frame = -2 / +3
Query: 396 ETGVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASS 217
E+GV GWRRPFVTHFTGCQPCSG RN YSGDSCD G+RRALNFADDQVLR YGFRHA
Sbjct: 105 ESGVKGWRRPFVTHFTGCQPCSGDRNRDYSGDSCDDGIRRALNFADDQVLRDYGFRHAGP 284
Query: 216 LSDD 205
LSDD
Sbjct: 285 LSDD 296
Score = 113 bits (241), Expect = 1e-024
Identities = 52/80 (65%), Positives = 53/80 (66%)
Frame = +2 / -1
Query: 203 TSSLSELACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLHG*HPVKCVTNGRRHP 382
TSSLS AC P +LSTWSSAKLSARRMP SQLSPE* R PLHG HPVKCVTNGR HP
Sbjct: 298 TSSLSGPACRNP*SLSTWSSAKLSARRMPSSQLSPE*SRFRSPLHGWHPVKCVTNGRLHP 119
Query: 383 CTXXXXXXXXXXXXFLATAY 442
T LA AY
Sbjct: 118 LTPLSASPAPASAASLACAY 59
>gb|CV062698.1|CV062698 BNEL82a1 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL82a1 5' similar to putative alpha
galactosyltransferase, mRNA sequence
Length = 298
Score = 142 bits (305), Expect = 2e-033
Identities = 54/64 (84%), Positives = 56/64 (87%)
Frame = -2 / +3
Query: 396 ETGVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASS 217
E+GV GWRRPFVTHFTGCQPCSG RN YSGDSCD G+RRALNFADDQVLR YGFRHA
Sbjct: 105 ESGVKGWRRPFVTHFTGCQPCSGDRNRDYSGDSCDDGIRRALNFADDQVLRDYGFRHAGP 284
Query: 216 LSDD 205
LSDD
Sbjct: 285 LSDD 296
Score = 113 bits (241), Expect = 1e-024
Identities = 52/80 (65%), Positives = 53/80 (66%)
Frame = +2 / -1
Query: 203 TSSLSELACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLHG*HPVKCVTNGRRHP 382
TSSLS AC P +LSTWSSAKLSARRMP SQLSPE* R PLHG HPVKCVTNGR HP
Sbjct: 298 TSSLSGPACRNP*SLSTWSSAKLSARRMPSSQLSPE*SRFRSPLHGWHPVKCVTNGRLHP 119
Query: 383 CTXXXXXXXXXXXXFLATAY 442
T LA AY
Sbjct: 118 LTPLSASPAPASAASLACAY 59
>gb|CV063833.1|CV063833 BNEL93f8 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL93f8 5' similar to putative alpha
galactosyltransferase, mRNA sequence
Length = 298
Score = 142 bits (305), Expect = 2e-033
Identities = 54/64 (84%), Positives = 56/64 (87%)
Frame = -2 / +3
Query: 396 ETGVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFRHASS 217
E+GV GWRRPFVTHFTGCQPCSG RN YSGDSCD G+RRALNFADDQVLR YGFRHA
Sbjct: 105 ESGVKGWRRPFVTHFTGCQPCSGDRNRDYSGDSCDDGIRRALNFADDQVLRDYGFRHAGP 284
Query: 216 LSDD 205
LSDD
Sbjct: 285 LSDD 296
Score = 113 bits (241), Expect = 1e-024
Identities = 52/80 (65%), Positives = 53/80 (66%)
Frame = +2 / -1
Query: 203 TSSLSELACLKPYALSTWSSAKLSARRMPWSQLSPE*CSLRCPLHG*HPVKCVTNGRRHP 382
TSSLS AC P +LSTWSSAKLSARRMP SQLSPE* R PLHG HPVKCVTNGR HP
Sbjct: 298 TSSLSGPACRNP*SLSTWSSAKLSARRMPSSQLSPE*SRFRSPLHGWHPVKCVTNGRLHP 119
Query: 383 CTXXXXXXXXXXXXFLATAY 442
T LA AY
Sbjct: 118 LTPLSASPAPASAASLACAY 59
>gb|CV058935.1|CV058935 BNEL42f10 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL42f10 5' similar to putative
alpha galactosyltransferase, mRNA sequence
Length = 303
Score = 125 bits (267), Expect = 3e-028
Identities = 47/56 (83%), Positives = 49/56 (87%)
Frame = -2 / +3
Query: 396 ETGVHGWRRPFVTHFTGCQPCSGQRNEHYSGDSCDQGMRRALNFADDQVLRAYGFR 229
E+GV GWRRPFVTHFT CQPCSG RN YSGDSCD G+RRALNFADDQVLR YGFR
Sbjct: 105 ESGVKGWRRPFVTHFTXCQPCSGDRNRDYSGDSCDDGIRRALNFADDQVLRDYGFR 272
>gb|CV057514.1|CV057514 BNEL28g10 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL28g10 5' similar to putative
alpha galactosyltransferase, mRNA sequence
Length = 602
Score = 105 bits (225), Expect(2) = 5e-025
Identities = 44/59 (74%), Positives = 47/59 (79%)
Frame = +1 / -2
Query: 475 QRGSSRPPLHRLVPLRHVGEPPHDLHPVALEVVAALQEHLVPPPAALLQQHVHERGLVV 651
QR RPPLHRLVP R EPPHDLHPVALEVVA LQEHL+PP AA + QHVH+R LVV
Sbjct: 472 QRWRRRPPLHRLVPRRDAAEPPHDLHPVALEVVAVLQEHLLPPRAAPVLQHVHQRRLVV 296
Score = 103 bits (220), Expect = 1e-021
Identities = 40/53 (75%), Positives = 45/53 (84%)
Frame = -2 / +2
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERYEAMERR 493
DDQSALVYML GS WR+KVFLE+GYYF+GYW+EIVGRL + RYEAMERR
Sbjct: 296 DDQSALVYMLQHRGSPWREKVFLENGYYFQGYWVEIVGRLGGIAARYEAMERR 454
Score = 28.1 bits (55), Expect(2) = 5e-025
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +1 / -2
Query: 364 ERAAPPVHPGLRQPGPAQGGVP 429
ERAAPP P LR+P Q +P
Sbjct: 589 ERAAPPFDPLLREPRXRQRRLP 524
>gb|BJ467007.1|BJ467007 BJ467007 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags35i03 3', mRNA sequence
Length = 653
Score = 64.3 bits (134), Expect(3) = 3e-023
Identities = 21/47 (44%), Positives = 32/47 (68%)
Frame = -2 / -3
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERY 511
DDQSAL+Y+LL + +W DKV++E+ YY G+W +V + M E +
Sbjct: 609 DDQSALIYLLLSQKEKWMDKVYIENSYYLHGFWAGLVDKYEEMMENH 469
Score = 46.0 bits (94), Expect(3) = 3e-023
Identities = 18/29 (62%), Positives = 20/29 (68%)
Frame = -2 / -3
Query: 312 YSGDSCDQGMRRALNFADDQVLRAYGFRH 226
Y D C + M RA NFAD+QVLR YGF H
Sbjct: 390 YPVDRCLKSMERAFNFADNQVLRLYGFAH 304
Score = 35.4 bits (71), Expect(3) = 3e-023
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = -2 / -3
Query: 390 GVHGWRRPFVTHFTGCQPC 334
G+ R PFVTHF GC+PC
Sbjct: 462 GLGDERWPFVTHFVGCKPC 406
>gb|CV058244.1|CV058244 BNEL35h4 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL35h4 5' similar to putative alpha
galactosyltransferase, mRNA sequence
Length = 559
Score = 105 bits (225), Expect = 2e-022
Identities = 44/59 (74%), Positives = 47/59 (79%)
Frame = +1 / -1
Query: 475 QRGSSRPPLHRLVPLRHVGEPPHDLHPVALEVVAALQEHLVPPPAALLQQHVHERGLVV 651
QR RPPLHRLVP R EPPHDLHPVALEVVA LQEHL+PP AA + QHVH+R LVV
Sbjct: 472 QRWRRRPPLHRLVPRRDAAEPPHDLHPVALEVVAVLQEHLLPPRAAPVLQHVHQRRLVV 296
Score = 103 bits (220), Expect = 1e-021
Identities = 40/53 (75%), Positives = 45/53 (84%)
Frame = -2 / +2
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERYEAMERR 493
DDQSALVYML GS WR+KVFLE+GYYF+GYW+EIVGRL + RYEAMERR
Sbjct: 296 DDQSALVYMLQHRGSPWREKVFLENGYYFQGYWVEIVGRLGGIAARYEAMERR 454
>gb|CV058639.1|CV058639 BNEL3C10 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL3C10 5' similar to putative alpha
galactosyltransferase, mRNA sequence
Length = 625
Score = 104 bits (221), Expect = 7e-022
Identities = 43/59 (72%), Positives = 46/59 (77%)
Frame = +1 / -1
Query: 475 QRGSSRPPLHRLVPLRHVGEPPHDLHPVALEVVAALQEHLVPPPAALLQQHVHERGLVV 651
QR RPP HRLVP R EPPHDLHPVALEVVA LQEHL+PP AA + QHVH+R LVV
Sbjct: 619 QRWGRRPPXHRLVPRRDAAEPPHDLHPVALEVVAVLQEHLLPPRAAPVLQHVHQRRLVV 443
Score = 100 bits (214), Expect = 7e-021
Identities = 39/53 (73%), Positives = 44/53 (83%)
Frame = -2 / +2
Query: 651 DDQSALVYMLLQEGSRWRDKVFLESGYYFEGYWMEIVGRLANMTERYEAMERR 493
DDQSALVYML GS WR+KVFLE+GYYF+GYW+EIVGRL + RYEAM RR
Sbjct: 443 DDQSALVYMLQHRGSPWREKVFLENGYYFQGYWVEIVGRLGGIAARYEAMXRR 601
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 312,069,953
Number of Sequences: 312970
Number of extensions: 4847118
Number of successful extensions: 59278
Number of sequences better than 1.0e-020: 28
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8144008128
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)