TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493751.2.1
(577 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ456617.1|BJ456617 BJ456617 K. Sato unpublished cDNA li... 116 3e-033
gb|U96096.1|HVU96096 Hordeum vulgare endo-1,3-beta-glucanas... 66 8e-030
gb|BM370310.2|BM370310 EBro08_SQ003_M21_R root, 3 week, dro... 66 2e-026
gb|BE060779.2|BE060779 HVSMEg0013F11f Hordeum vulgare pre-a... 86 2e-026
gb|BM370290.2|BM370290 EBro08_SQ003_L21_R root, 3 week, dro... 82 3e-025
gb|BU981210.1|BU981210 HA22P03r HA Hordeum vulgare subsp. v... 95 7e-025
gb|BU980481.1|BU980481 HA20M14r HA Hordeum vulgare subsp. v... 95 7e-025
gb|BQ469894.1|BQ469894 HX01C20T HX Hordeum vulgare subsp. v... 76 7e-025
gb|BU981251.1|BU981251 HA23B03r HA Hordeum vulgare subsp. v... 95 7e-025
gb|BF265292.2|BF265292 HV_CEa0011J05f Hordeum vulgare seedl... 74 2e-024
gb|BM816843.1|BM816843 HC03E07_T3.ab1 HC Hordeum vulgare su... 72 1e-022
gb|BG416675.1|BG416675 HVSMEk0014B06f Hordeum vulgare testa... 72 1e-022
gb|CA017450.1|CA017450 HV05D22r HV Hordeum vulgare subsp. v... 66 2e-022
gb|CK125687.1|CK125687 BES1824110a22 BES1824 Hordeum vulgar... 87 2e-022
gb|AV925542.1|AV925542 AV925542 K. Sato unpublished cDNA li... 105 2e-022
gb|AV926710.1|AV926710 AV926710 K. Sato unpublished cDNA li... 105 2e-022
gb|BQ465340.1|BQ465340 HU03F15r HU Hordeum vulgare subsp. v... 105 2e-022
gb|CA018533.1|CA018533 HV08O12r HV Hordeum vulgare subsp. v... 105 2e-022
emb|X52572.1|HVBDG Barley DNA for (1-3,1-4)-beta-D-glucanas... 105 2e-022
emb|X56775.1|HVGLB1 H.vulgare Glb 1 gene for 1-3,1-4-beta-D... 105 2e-022
emb|X56260.1|HVGLUEND Barley DNA for (1,3;1,4)-beta-glucana... 105 2e-022
gb|AV931182.1|AV931182 AV931182 K. Sato unpublished cDNA li... 105 3e-022
gb|BJ545156.1|BJ545156 BJ545156 K. Sato unpublished cDNA li... 104 4e-022
gb|AV923914.1|AV923914 AV923914 K. Sato unpublished cDNA li... 104 5e-022
gb|BM816983.1|BM816983 HC113B09_SK.ab1 HC Hordeum vulgare s... 52 1e-021
gb|BI947339.1|BI947339 HVSMEl0005D01f Hordeum vulgare spike... 102 1e-021
gb|BF263602.2|BF263602 HV_CEa0007C20f Hordeum vulgare seedl... 65 2e-021
gb|BF265541.2|BF265541 HV_CEa0012I19f Hordeum vulgare seedl... 101 3e-021
gb|BQ760714.1|BQ760714 EBro03_SQ003_E08_R root, 3 week, wat... 90 3e-021
gb|AL504555.1|AL504555 AL504555 Hordeum vulgare Barke roots... 69 4e-021
>gb|BJ456617.1|BJ456617 BJ456617 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak22k17 3', mRNA sequence
Length = 710
Score = 116 bits (247), Expect(2) = 3e-033
Identities = 49/100 (49%), Positives = 61/100 (61%)
Frame = +2 / -2
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFA 187
YP+ S+V D A + + YALF + VQDGA Y NLFDA VD +ALE G G V
Sbjct: 634 YPYFSYVRDQAQIDINYALFKSQGTVVQDGANAYQNLFDAIVDTFYSALESAGAGSVGVV 455
Query: 188 VTETGWPTAGHPAATPQNATAYNAKIVERPARGVGTPKRP 307
V+E+GWP+ G AAT NA YN +++ G GTPKRP
Sbjct: 454 VSESGWPSEGGTAATTDNAQTYNQNLIKHIHVGQGTPKRP 335
Score = 45.5 bits (93), Expect(2) = 3e-033
Identities = 16/38 (42%), Positives = 26/38 (68%)
Frame = +2 / -2
Query: 317 VEVFLFDLYDEDGKPGPEFERHFGIFRADGSKAYDINF 430
+E ++F +++E+ K E E+HFG+F D S AY I+F
Sbjct: 328 IEAYVFAMFNENRKGPAEIEKHFGLFNPDKSPAYPISF 215
>gb|U96096.1|HVU96096 Hordeum vulgare endo-1,3-beta-glucanase (Hv34) gene, partial cds
Length = 2899
Score = 66.1 bits (138), Expect(4) = 8e-030
Identities = 27/46 (58%), Positives = 33/46 (71%)
Frame = +2 / +1
Query: 80 APVQDGALVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAG 217
A V DG LVYTN+FDA +DA AA+EK G G+ V+ETGWP+ G
Sbjct: 2326 ASVTDGGLVYTNMFDAILDAAHAAVEKAGAQGLELVVSETGWPSGG 2463
Score = 59.7 bits (124), Expect(4) = 8e-030
Identities = 22/49 (44%), Positives = 32/49 (65%)
Frame = +2 / +1
Query: 215 GHPAATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDEDGKP 361
G AT +NA AYN ++ A G GTP+RP + VE +LF +++E+ KP
Sbjct: 2464 GGTGATVENAAAYNNNVIRHAASGAGTPRRPGKAVETYLFAMFNENQKP 2610
Score = 30.4 bits (60), Expect(4) = 8e-030
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +2 / +1
Query: 374 ERHFGIFRADGSKAYDINFA 433
E+HFG+F+ D S Y ++FA
Sbjct: 2620 EQHFGLFQPDMSAVYPVDFA 2679
Score = 28.6 bits (56), Expect(4) = 8e-030
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +2 / +1
Query: 8 YPFISHVNDPANVQLAYALFGAGAA 82
YP+ ++ +P VQL YAL ++
Sbjct: 2224 YPYFAYAAEPETVQLGYALLAGSSS 2298
>gb|BM370310.2|BM370310 EBro08_SQ003_M21_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ003_M21 5', mRNA sequence
Length = 655
Score = 66.1 bits (138), Expect(3) = 2e-026
Identities = 27/46 (58%), Positives = 33/46 (71%)
Frame = +2 / +1
Query: 80 APVQDGALVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAG 217
A V DG LVYTN+FDA +DA AA+EK G G+ V+ETGWP+ G
Sbjct: 43 ASVTDGGLVYTNMFDAILDAAHAAVEKAGAQGLELVVSETGWPSGG 180
Score = 59.7 bits (124), Expect(3) = 2e-026
Identities = 22/49 (44%), Positives = 32/49 (65%)
Frame = +2 / +1
Query: 215 GHPAATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDEDGKP 361
G AT +NA AYN ++ A G GTP+RP + VE +LF +++E+ KP
Sbjct: 181 GGTGATVENAAAYNNNVIRHAASGAGTPRRPGKAVETYLFAMFNENQKP 327
Score = 30.4 bits (60), Expect(3) = 2e-026
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +2 / +1
Query: 374 ERHFGIFRADGSKAYDINFA 433
E+HFG+F+ D S Y ++FA
Sbjct: 337 EQHFGLFQPDMSAVYPVDFA 396
>gb|BE060779.2|BE060779 HVSMEg0013F11f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0013F11f, mRNA sequence
Length = 688
Score = 85.8 bits (181), Expect(2) = 2e-026
Identities = 33/67 (49%), Positives = 47/67 (70%)
Frame = +2 / +1
Query: 227 ATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDEDGKPGPEFERHFGIFRADG 406
AT QNA AYN +++R A GTP +P ++VF+F L++E+ KPGP FER++G+F +G
Sbjct: 265 ATAQNAAAYNGNLMQRIAMSQGTPLKPSVPIDVFVFALFNENMKPGPAFERNYGLFYPNG 444
Query: 407 SKAYDIN 427
S Y IN
Sbjct: 445 SPVYAIN 465
Score = 52.4 bits (108), Expect(2) = 2e-026
Identities = 21/46 (45%), Positives = 30/46 (65%)
Frame = +2 / +1
Query: 80 APVQDGALVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAG 217
A V+DG LVY N+ A +DA+ AA++ G + V+ETGWP+ G
Sbjct: 109 AGVRDGGLVYDNMLYAQIDAVYAAMKAMGHADIGVRVSETGWPSKG 246
>gb|BM370290.2|BM370290 EBro08_SQ003_L21_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ003_L21 5', mRNA sequence
Length = 630
Score = 82.2 bits (173), Expect(2) = 3e-025
Identities = 32/67 (47%), Positives = 46/67 (68%)
Frame = +2 / +1
Query: 227 ATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDEDGKPGPEFERHFGIFRADG 406
AT QNA AYN +++R A GTP +P ++VF+F L++E+ KPGP ER++G+F +G
Sbjct: 202 ATAQNAAAYNGNLMQRIAMSQGTPLKPSVPIDVFVFALFNENMKPGPASERNYGLFYPNG 381
Query: 407 SKAYDIN 427
S Y IN
Sbjct: 382 SPVYAIN 402
Score = 52.4 bits (108), Expect(2) = 3e-025
Identities = 21/46 (45%), Positives = 30/46 (65%)
Frame = +2 / +1
Query: 80 APVQDGALVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAG 217
A V+DG LVY N+ A +DA+ AA++ G + V+ETGWP+ G
Sbjct: 46 AGVRDGGLVYDNMLYAQIDAVYAAMKAMGHADIGVRVSETGWPSKG 183
>gb|BU981210.1|BU981210 HA22P03r HA Hordeum vulgare subsp. vulgare cDNA clone HA22P03
5-PRIME, mRNA sequence
Length = 566
Score = 95.0 bits (201), Expect(2) = 7e-025
Identities = 45/90 (50%), Positives = 54/90 (60%)
Frame = +2 / +3
Query: 68 GAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAGHPAATPQNAT 247
GA A VYTNLFDA VDA+ AALEK G GV V+ETGWP+AG AATP+NA
Sbjct: 174 GATAVRDAGNGHVYTNLFDAMVDAMYAALEKAGAPGVRVVVSETGWPSAGGFAATPENAR 353
Query: 248 AYNAKIVERPARGVGTPKRPKETVEVFLFD 337
AYN +++ ARG P E +F+
Sbjct: 354 AYNQGMIDHVARGTPNRPGPIEAYVFAMFN 443
Score = 38.2 bits (77), Expect(2) = 7e-025
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +2 / +3
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQD 94
YP+I++ N P ++QL YA F GA V+D
Sbjct: 108 YPYIAYRNSPRDIQLNYATFQPGATAVRD 194
>gb|BU980481.1|BU980481 HA20M14r HA Hordeum vulgare subsp. vulgare cDNA clone HA20M14
5-PRIME, mRNA sequence
Length = 502
Score = 95.0 bits (201), Expect(2) = 7e-025
Identities = 45/90 (50%), Positives = 54/90 (60%)
Frame = +2 / +3
Query: 68 GAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAGHPAATPQNAT 247
GA A VYTNLFDA VDA+ AALEK G GV V+ETGWP+AG AATP+NA
Sbjct: 207 GATAVRDAGNGHVYTNLFDAMVDAMYAALEKAGAPGVRVVVSETGWPSAGGFAATPENAR 386
Query: 248 AYNAKIVERPARGVGTPKRPKETVEVFLFD 337
AYN +++ ARG P E +F+
Sbjct: 387 AYNQGMIDHVARGTPNRPGPIEAYVFAMFN 476
Score = 38.2 bits (77), Expect(2) = 7e-025
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +2 / +3
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQD 94
YP+I++ N P ++QL YA F GA V+D
Sbjct: 141 YPYIAYRNSPRDIQLNYATFQPGATAVRD 227
>gb|BQ469894.1|BQ469894 HX01C20T HX Hordeum vulgare subsp. vulgare cDNA clone HX01C20
5-PRIME, mRNA sequence
Length = 496
Score = 76.2 bits (160), Expect(2) = 7e-025
Identities = 30/67 (44%), Positives = 43/67 (64%)
Frame = +2 / +2
Query: 227 ATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDEDGKPGPEFERHFGIFRADG 406
A +NA YN + R AR GTP RP + VF+F LY+E+ KPGP ERH+G++ +G
Sbjct: 191 ANVRNAAVYNRNLAARMARNPGTPARPGARMPVFVFSLYNENLKPGPGTERHWGLYYPNG 370
Query: 407 SKAYDIN 427
+ AY ++
Sbjct: 371 TAAYRVD 391
Score = 57.0 bits (118), Expect(2) = 7e-025
Identities = 23/39 (58%), Positives = 26/39 (66%)
Frame = +2 / +2
Query: 101 LVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAG 217
L YTNL D +DA+ AA+ K G GGV AV ETGWP G
Sbjct: 56 LTYTNLLDEMLDAVGAAMSKLGHGGVRIAVAETGWPNGG 172
>gb|BU981251.1|BU981251 HA23B03r HA Hordeum vulgare subsp. vulgare cDNA clone HA23B03
5-PRIME, mRNA sequence
Length = 487
Score = 95.0 bits (201), Expect(2) = 7e-025
Identities = 45/90 (50%), Positives = 54/90 (60%)
Frame = +2 / +3
Query: 68 GAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAGHPAATPQNAT 247
GA A VYTNLFDA VDA+ AALEK G GV V+ETGWP+AG AATP+NA
Sbjct: 174 GATAVRDAGNGHVYTNLFDAMVDAMYAALEKAGAPGVRVVVSETGWPSAGGFAATPENAR 353
Query: 248 AYNAKIVERPARGVGTPKRPKETVEVFLFD 337
AYN +++ ARG P E +F+
Sbjct: 354 AYNQGMIDHVARGTPNRPGPIEAYVFAMFN 443
Score = 38.2 bits (77), Expect(2) = 7e-025
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +2 / +3
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQD 94
YP+I++ N P ++QL YA F GA V+D
Sbjct: 108 YPYIAYRNSPRDIQLNYATFQPGATAVRD 194
>gb|BF265292.2|BF265292 HV_CEa0011J05f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0011J05f, mRNA sequence
Length = 523
Score = 74.4 bits (156), Expect(2) = 2e-024
Identities = 29/67 (43%), Positives = 42/67 (62%)
Frame = +2 / +1
Query: 227 ATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDEDGKPGPEFERHFGIFRADG 406
A +NA YN + R AR GTP RP + VF+F LY+E+ KPGP ERH+G++ +G
Sbjct: 136 ANVRNAAVYNRNLAARMARNPGTPARPGARMPVFVFSLYNENLKPGPGTERHWGLYYPNG 315
Query: 407 SKAYDIN 427
+ Y ++
Sbjct: 316 TAVYRVD 336
Score = 57.0 bits (118), Expect(2) = 2e-024
Identities = 23/39 (58%), Positives = 26/39 (66%)
Frame = +2 / +1
Query: 101 LVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAG 217
L YTNL D +DA+ AA+ K G GGV AV ETGWP G
Sbjct: 1 LTYTNLLDEMLDAVGAAMSKLGHGGVRIAVAETGWPNGG 117
>gb|BM816843.1|BM816843 HC03E07_T3.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC03E07_T3.ab1 similar to Glucosylceramidase,Glucan
endo-1,2-beta-glucosidase,Glucan
endo-1,6-beta-glucosidase, mRNA sequence
Length = 833
Score = 71.6 bits (150), Expect(2) = 1e-022
Identities = 28/63 (44%), Positives = 38/63 (60%)
Frame = +2 / +3
Query: 236 QNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDEDGKPGPEFERHFGIFRADGSKA 415
Q A +N ++ + G GTP P T E +LF L+DE+ KPGP ERHFG+F D +
Sbjct: 252 QEARDFNEGMIRVCSSGKGTPLMPNRTFETYLFSLFDENQKPGPIAERHFGLFNPDFTPV 431
Query: 416 YDI 424
YD+
Sbjct: 432 YDL 440
Score = 54.2 bits (112), Expect(2) = 1e-022
Identities = 21/41 (51%), Positives = 28/41 (68%)
Frame = +2 / +3
Query: 101 LVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAGHP 223
L YT++FDA +DA+ A++K G+G V AV E GWPT P
Sbjct: 108 LNYTSMFDAQMDAIYTAMKKLGYGDVDIAVGEAGWPTQAEP 230
>gb|BG416675.1|BG416675 HVSMEk0014B06f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0014B06f, mRNA sequence
Length = 695
Score = 71.6 bits (150), Expect(2) = 1e-022
Identities = 28/63 (44%), Positives = 38/63 (60%)
Frame = +2 / +2
Query: 236 QNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDEDGKPGPEFERHFGIFRADGSKA 415
Q A +N ++ + G GTP P T E +LF L+DE+ KPGP ERHFG+F D +
Sbjct: 239 QEARDFNEGMIRVCSSGKGTPLMPNRTFETYLFSLFDENQKPGPIAERHFGLFNPDFTPV 418
Query: 416 YDI 424
YD+
Sbjct: 419 YDL 427
Score = 54.2 bits (112), Expect(2) = 1e-022
Identities = 21/41 (51%), Positives = 28/41 (68%)
Frame = +2 / +2
Query: 101 LVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAGHP 223
L YT++FDA +DA+ A++K G+G V AV E GWPT P
Sbjct: 95 LNYTSMFDAQMDAIYTAMKKLGYGDVDIAVGEAGWPTQAEP 217
>gb|CA017450.1|CA017450 HV05D22r HV Hordeum vulgare subsp. vulgare cDNA clone HV05D22
5-PRIME, mRNA sequence
Length = 492
Score = 66.1 bits (138), Expect(3) = 2e-022
Identities = 27/46 (58%), Positives = 33/46 (71%)
Frame = +2 / +3
Query: 80 APVQDGALVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAG 217
A V DG LVYTN+FDA +DA AA+EK G G+ V+ETGWP+ G
Sbjct: 237 ASVTDGGLVYTNMFDAILDAAHAAVEKAGAQGLELVVSETGWPSGG 374
Score = 47.8 bits (98), Expect(3) = 2e-022
Identities = 18/39 (46%), Positives = 24/39 (61%)
Frame = +2 / +3
Query: 215 GHPAATPQNATAYNAKIVERPARGVGTPKRPKETVEVFL 331
G AT +NA AYN ++ A G GTP+RP + VE +L
Sbjct: 375 GGTGATVENAAAYNNNVIRHAASGAGTPRRPGKAVETYL 491
Score = 28.6 bits (56), Expect(3) = 2e-022
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +2 / +3
Query: 8 YPFISHVNDPANVQLAYALFGAGAA 82
YP+ ++ +P VQL YAL ++
Sbjct: 135 YPYFAYAAEPETVQLGYALLAGSSS 209
>gb|CK125687.1|CK125687 BES1824110a22 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010A2210 5-PRIME, mRNA sequence
Length = 484
Score = 86.8 bits (183), Expect(2) = 2e-022
Identities = 39/68 (57%), Positives = 46/68 (67%)
Frame = +2 / +2
Query: 68 GAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAGHPAATPQNAT 247
GA A VYTNLFDA VDA+ AALEK G GV V+ETGWP+AG AATP+NA
Sbjct: 140 GATAVRDAGNGHVYTNLFDAMVDAMYAALEKAGAPGVRXVVSETGWPSAGGFAATPENAR 319
Query: 248 AYNAKIVE 271
AYN +++
Sbjct: 320 AYNQGMID 343
Score = 38.2 bits (77), Expect(2) = 2e-022
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +2 / +2
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQD 94
YP+I++ N P ++QL YA F GA V+D
Sbjct: 74 YPYIAYRNSPRDIQLNYATFQPGATAVRD 160
>gb|AV925542.1|AV925542 AV925542 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd24m02 5', mRNA sequence
Length = 643
Score = 105 bits (224), Expect = 2e-022
Identities = 46/115 (40%), Positives = 64/115 (55%)
Frame = +2 / +1
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFA 187
YP+++ +P+ + ++YALF A VQDG+ Y NLFD TVDA A+ K G V
Sbjct: 103 YPYLAWAYNPSAMDMSYALFTASGTVVQDGSYGYQNLFDTTVDAFYTAMAKHGGSNVKLV 282
Query: 188 VTETGWPTAGHPAATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDED 352
V+E+GWP+AG AATP NA YN ++ RG ET +F+ +D
Sbjct: 283 VSESGWPSAGGTAATPANARIYNQYLINHVGRGTPRHPGAIETYVFSMFNENQKD 447
>gb|AV926710.1|AV926710 AV926710 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd23h17 5', mRNA sequence
Length = 690
Score = 105 bits (224), Expect = 2e-022
Identities = 46/115 (40%), Positives = 64/115 (55%)
Frame = +2 / +2
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFA 187
YP+++ +P+ + ++YALF A VQDG+ Y NLFD TVDA A+ K G V
Sbjct: 194 YPYLAWAYNPSAMDMSYALFTASGTVVQDGSYGYQNLFDTTVDAFYTAMAKHGGSNVKLV 373
Query: 188 VTETGWPTAGHPAATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDED 352
V+E+GWP+AG AATP NA YN ++ RG ET +F+ +D
Sbjct: 374 VSESGWPSAGGTAATPANARIYNQYLINHVGRGTPRHPGAIETYVFSMFNENQKD 538
>gb|BQ465340.1|BQ465340 HU03F15r HU Hordeum vulgare subsp. vulgare cDNA clone HU03F15
5-PRIME, mRNA sequence
Length = 626
Score = 105 bits (224), Expect = 2e-022
Identities = 46/115 (40%), Positives = 64/115 (55%)
Frame = +2 / +3
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFA 187
YP+++ +P+ + ++YALF A VQDG+ Y NLFD TVDA A+ K G V
Sbjct: 138 YPYLAWAYNPSAMDMSYALFTASGTVVQDGSYGYQNLFDTTVDAFYTAMAKHGGSNVKLV 317
Query: 188 VTETGWPTAGHPAATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDED 352
V+E+GWP+AG AATP NA YN ++ RG ET +F+ +D
Sbjct: 318 VSESGWPSAGGTAATPANARIYNQYLINHVGRGTPRHPGAIETYVFSMFNENQKD 482
>gb|CA018533.1|CA018533 HV08O12r HV Hordeum vulgare subsp. vulgare cDNA clone HV08O12
5-PRIME, mRNA sequence
Length = 645
Score = 105 bits (224), Expect = 2e-022
Identities = 46/115 (40%), Positives = 64/115 (55%)
Frame = +2 / +2
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFA 187
YP+++ +P+ + ++YALF A VQDG+ Y NLFD TVDA A+ K G V
Sbjct: 221 YPYLAWAYNPSAMDMSYALFTASGTVVQDGSYGYQNLFDTTVDAFYTAMAKHGGSNVKLV 400
Query: 188 VTETGWPTAGHPAATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDED 352
V+E+GWP+AG AATP NA YN ++ RG ET +F+ +D
Sbjct: 401 VSESGWPSAGGTAATPANARIYNQYLINHVGRGTPRHPGAIETYVFSMFNENQKD 565
>emb|X52572.1|HVBDG Barley DNA for (1-3,1-4)-beta-D-glucanase (EC 3.2.1.73)
Length = 6260
Score = 105 bits (224), Expect = 2e-022
Identities = 46/115 (40%), Positives = 64/115 (55%)
Frame = +2 / +3
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFA 187
YP+++ +P+ + ++YALF A VQDG+ Y NLFD TVDA A+ K G V
Sbjct: 5058 YPYLAWAYNPSAMDMSYALFTASGTVVQDGSYGYQNLFDTTVDAFYTAMAKHGGSNVKLV 5237
Query: 188 VTETGWPTAGHPAATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDED 352
V+E+GWP+AG AATP NA YN ++ RG ET +F+ +D
Sbjct: 5238 VSESGWPSAGGTAATPANARIYNQYLINHVGRGTPRHPGAIETYVFSMFNENQKD 5402
>emb|X56775.1|HVGLB1 H.vulgare Glb 1 gene for 1-3,1-4-beta-D-glucanase
Length = 4849
Score = 105 bits (224), Expect = 2e-022
Identities = 46/115 (40%), Positives = 64/115 (55%)
Frame = +2 / +1
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFA 187
YP+++ +P+ + ++YALF A VQDG+ Y NLFD TVDA A+ K G V
Sbjct: 4051 YPYLAWAYNPSAMDMSYALFTASGTVVQDGSYGYQNLFDTTVDAFYTAMAKHGGSNVKLV 4230
Query: 188 VTETGWPTAGHPAATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDED 352
V+E+GWP+AG AATP NA YN ++ RG ET +F+ +D
Sbjct: 4231 VSESGWPSAGGTAATPANARIYNQYLINHVGRGTPRHPGAIETYVFSMFNENQKD 4395
>emb|X56260.1|HVGLUEND Barley DNA for (1,3;1,4)-beta-glucanase.(EC number 3.2.1.73)
Length = 4643
Score = 105 bits (224), Expect = 2e-022
Identities = 46/115 (40%), Positives = 64/115 (55%)
Frame = +2 / +3
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFA 187
YP+++ +P+ + ++YALF A VQDG+ Y NLFD TVDA A+ K G V
Sbjct: 3651 YPYLAWAYNPSAMDMSYALFTASGTVVQDGSYGYQNLFDTTVDAFYTAMAKHGGSNVKLV 3830
Query: 188 VTETGWPTAGHPAATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDED 352
V+E+GWP+AG AATP NA YN ++ RG ET +F+ +D
Sbjct: 3831 VSESGWPSAGGTAATPANARIYNQYLINHVGRGTPRHPGAIETYVFSMFNENQKD 3995
>gb|AV931182.1|AV931182 AV931182 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd18n18 3', mRNA sequence
Length = 690
Score = 105 bits (223), Expect = 3e-022
Identities = 46/116 (39%), Positives = 66/116 (56%)
Frame = +2 / -3
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFA 187
YP+ ++ +P ++QL++A F G+ V D L YTNLFDA VD++ AALEK G GV
Sbjct: 580 YPYFAYKGNPRDIQLSFATFVPGSTTVNDNGLTYTNLFDAMVDSIYAALEKAGTPGVKVV 401
Query: 188 VTETGWPTAGHPAATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDEDG 355
++E+GWP+ AT QNA AYN ++ G ET +F+ +DG
Sbjct: 400 ISESGWPSDQGFGATAQNARAYNQGLINHVGNGTPKKSGALETYIFAMFNENLKDG 233
>gb|BJ545156.1|BJ545156 BJ545156 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak45b03 5', mRNA sequence
Length = 554
Score = 104 bits (222), Expect = 4e-022
Identities = 45/110 (40%), Positives = 62/110 (56%)
Frame = +2 / +1
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFA 187
YP+++ +P+ + ++YALF A VQDG+ Y NLFD TVDA A+ K G V
Sbjct: 217 YPYLAWAYNPSAMDMSYALFTASGTVVQDGSYGYQNLFDTTVDAFYTAMAKHGGSNVKLV 396
Query: 188 VTETGWPTAGHPAATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFD 337
V+E+GWP+AG AATP NA YN ++ RG ET +F+
Sbjct: 397 VSESGWPSAGGTAATPANARIYNQYLINHVGRGTPRHPGAIETYVFSMFN 546
>gb|AV923914.1|AV923914 AV923914 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd20b23 5', mRNA sequence
Length = 520
Score = 104 bits (221), Expect = 5e-022
Identities = 42/93 (45%), Positives = 57/93 (61%)
Frame = +2 / +2
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFA 187
YP+++ +P+ + ++YALF A VQDG+ Y NLFD TVDA A+ K G V
Sbjct: 215 YPYLAWAYNPSAMDMSYALFTASGTVVQDGSYGYQNLFDTTVDAFYTAMAKHGGSNVKLV 394
Query: 188 VTETGWPTAGHPAATPQNATAYNAKIVERPARG 286
V+E+GWP+AG AATP NA YN ++ RG
Sbjct: 395 VSESGWPSAGGTAATPANARIYNQYLINHVGRG 493
>gb|BM816983.1|BM816983 HC113B09_SK.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC113B09_SK.ab1 similar to
Glucosylceramidase,licheninase,Glucan endo-1, mRNA
sequence
Length = 919
Score = 52.4 bits (108), Expect(3) = 1e-021
Identities = 21/46 (45%), Positives = 30/46 (65%)
Frame = +2 / +2
Query: 80 APVQDGALVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAG 217
A V+DG LVY N+ A +DA+ AA++ G + V+ETGWP+ G
Sbjct: 257 AGVRDGGLVYDNMLYAQIDAVYAAMKAMGHADIGVRVSETGWPSKG 394
Score = 51.0 bits (105), Expect(3) = 1e-021
Identities = 19/41 (46%), Positives = 29/41 (70%)
Frame = +2 / +3
Query: 305 PKETVEVFLFDLYDEDGKPGPEFERHFGIFRADGSKAYDIN 427
P ++VF+F L++E+ KPGP ER++G+F +GS Y IN
Sbjct: 492 PACXIDVFVFALFNENMKPGPASERNYGLFYPNGSPVYAIN 614
Score = 36.3 bits (73), Expect(3) = 1e-021
Identities = 14/27 (51%), Positives = 18/27 (66%)
Frame = +2 / +2
Query: 227 ATPQNATAYNAKIVERPARGVGTPKRP 307
AT QNA AYN +++R A GTP +P
Sbjct: 413 ATAQNAAAYNGNLMQRIAMSQGTPLKP 493
>gb|BI947339.1|BI947339 HVSMEl0005D01f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0005D01f, mRNA sequence
Length = 484
Score = 102 bits (218), Expect = 1e-021
Identities = 42/93 (45%), Positives = 57/93 (61%)
Frame = +2 / +3
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFA 187
YP+++ +P+ + ++YALF A VQDG+ Y NLFD TVDA A+ K G V
Sbjct: 198 YPYLAWAYNPSAMDMSYALFTASGTVVQDGSYGYQNLFDTTVDAFYTAMAKHGGSNVKLV 377
Query: 188 VTETGWPTAGHPAATPQNATAYNAKIVERPARG 286
V+E+GWP+AG AATP NA YN ++ RG
Sbjct: 378 VSESGWPSAGGTAATPANARIYNQYLINHVWRG 476
>gb|BF263602.2|BF263602 HV_CEa0007C20f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0007C20f, mRNA sequence
Length = 816
Score = 64.8 bits (135), Expect(2) = 2e-021
Identities = 27/60 (45%), Positives = 35/60 (58%)
Frame = +2 / +2
Query: 227 ATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDEDGKPGPEFERHFGIFRADG 406
A NA YN + ER AR GTP RP + +F LY+E+ KPGP ERH+G++ G
Sbjct: 506 ANVPNAXVYNRNLAERMARNPGTPTRPGARMPRVVFSLYNENLKPGPGTERHWGLYYPKG 685
Score = 57.0 bits (118), Expect(2) = 2e-021
Identities = 23/39 (58%), Positives = 26/39 (66%)
Frame = +2 / +2
Query: 101 LVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAG 217
L YTNL D +DA+ AA+ K G GGV AV ETGWP G
Sbjct: 371 LTYTNLLDEMLDAVGAAMSKLGHGGVRIAVAETGWPNGG 487
>gb|BF265541.2|BF265541 HV_CEa0012I19f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0012I19f, mRNA sequence
Length = 751
Score = 101 bits (216), Expect = 3e-021
Identities = 41/93 (44%), Positives = 56/93 (60%)
Frame = +2 / +3
Query: 8 YPFISHVNDPANVQLAYALFGAGAAPVQDGALVYTNLFDATVDALVAALEKEGFGGVLFA 187
YP+++ +P+ + ++YALF A VQDG+ Y NLFD TVDA A+ K G V
Sbjct: 468 YPYLAWAYNPSAMDMSYALFTASGTVVQDGSYGYQNLFDTTVDAFYTAMAKHGGSNVKLV 647
Query: 188 VTETGWPTAGHPAATPQNATAYNAKIVERPARG 286
V+E+GWP+AG ATP NA YN ++ RG
Sbjct: 648 VSESGWPSAGGTVATPANARNYNPVLINHVGRG 746
>gb|BQ760714.1|BQ760714 EBro03_SQ003_E08_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ003_E08 5', mRNA sequence
Length = 412
Score = 90.4 bits (191), Expect(2) = 3e-021
Identities = 37/74 (50%), Positives = 48/74 (64%)
Frame = +2 / +2
Query: 212 AGHPAATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDEDGKPGPEFERHFGI 391
AG ATP NA AYN +V VGTP+ P ++V+ ++F LYDED KPGP ER FG+
Sbjct: 74 AGEAGATPDNARAYNGNLVAHLRSQVGTPRTPGKSVDTYIFALYDEDLKPGPVSERSFGL 253
Query: 392 FRADGSKAYDINFA 433
++AD + YDI A
Sbjct: 254 YQADLTANYDIGLA 295
Score = 30.4 bits (60), Expect(2) = 3e-021
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = +2 / +2
Query: 149 ALEKEGFGGVLFAVTETGWPTAG 217
AL+ +G GV V ETGWP G
Sbjct: 2 ALDGKGCAGVDIVVAETGWPYKG 70
>gb|AL504555.1|AL504555 AL504555 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW05K08V 5', mRNA sequence
Length = 663
Score = 68.9 bits (144), Expect(3) = 4e-021
Identities = 26/66 (39%), Positives = 42/66 (63%)
Frame = +2 / +3
Query: 227 ATPQNATAYNAKIVERPARGVGTPKRPKETVEVFLFDLYDEDGKPGPEFERHFGIFRADG 406
ATP++A Y ++ R GTP RP ++V++F L++E+ KPGP ER++G+F DG
Sbjct: 291 ATPEHAGTYIGNLLRRIEMKQGTPLRPAVPIDVYVFALFNENLKPGPASERNYGLFYPDG 470
Query: 407 SKAYDI 424
Y++
Sbjct: 471 KPVYNV 488
Score = 41.4 bits (84), Expect(3) = 4e-021
Identities = 18/41 (43%), Positives = 25/41 (60%)
Frame = +2 / +3
Query: 101 LVYTNLFDATVDALVAALEKEGFGGVLFAVTETGWPTAGHP 223
L Y N+ A VDA+ +A++ G V V+ETGWP+ G P
Sbjct: 156 LNYDNMLYAQVDAVYSAIKALGHTDVDVKVSETGWPSRGDP 278
Score = 27.6 bits (54), Expect(3) = 4e-021
Identities = 10/24 (41%), Positives = 13/24 (54%)
Frame = +2 / +3
Query: 8 YPFISHVNDPANVQLAYALFGAGA 79
YPF ++ +DP V Y LF A
Sbjct: 60 YPFFAYKDDPXRVPXEYVLFQPNA 131
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 195,870,064
Number of Sequences: 312970
Number of extensions: 2386731
Number of successful extensions: 46044
Number of sequences better than 1.0e-020: 40
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 5938339260
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)