TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2478337.2.1
(729 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AJ463236.1|AJ463236 AJ463236 S00002 Hordeum vulgare subs... 177 8e-044
gb|BF257093.3|BF257093 HVSMEf0011N11f Hordeum vulgare seedl... 137 4e-038
gb|BI950777.1|BI950777 HVSMEl0023C15f Hordeum vulgare spike... 87 3e-035
gb|AJ463237.1|AJ463237 AJ463237 S00002 Hordeum vulgare subs... 138 3e-032
gb|BU980984.1|BU980984 HA22E18r HA Hordeum vulgare subsp. v... 82 7e-032
gb|BM098205.2|BM098205 EBpi03_SQ002_N14_R pistil, 4 DPA, no... 82 7e-032
gb|CB880652.1|CB880652 HM07E17w HM Hordeum vulgare subsp. v... 82 1e-029
gb|CB881234.1|CB881234 HM09C23w HM Hordeum vulgare subsp. v... 82 1e-029
gb|BQ759881.1|BQ759881 EBpi05_SQ004_P16_R pistil, 8 DPA, no... 82 1e-029
gb|AV928430.1|AV928430 AV928430 K. Sato unpublished cDNA li... 72 1e-026
gb|BQ467895.1|BQ467895 HR01E04r HR Hordeum vulgare subsp. v... 72 1e-026
gb|BU986289.1|BU986289 HF11E17r HF Hordeum vulgare subsp. v... 72 1e-026
gb|BF260026.3|BF260026 HVSMEf0020O13f Hordeum vulgare seedl... 72 1e-026
gb|CA019924.1|CA019924 HV13L18r HV Hordeum vulgare subsp. v... 116 2e-025
gb|BJ463021.1|BJ463021 BJ463021 K. Sato unpublished cDNA li... 87 2e-025
gb|BG369116.2|BG369116 HVSMEi0021M10f Hordeum vulgare 20 DA... 55 3e-023
gb|CK124171.1|CK124171 BES1824107e17 BES1824 Hordeum vulgar... 64 4e-023
gb|CK122346.1|CK122346 BES1824102p12 BES1824 Hordeum vulgar... 64 4e-023
gb|BE060246.3|BE060246 HVSMEg0011L08f Hordeum vulgare pre-a... 64 4e-023
gb|BQ459628.1|BQ459628 HA08J01r HA Hordeum vulgare subsp. v... 64 4e-023
gb|AV917139.1|AV917139 AV917139 K. Sato unpublished cDNA li... 64 1e-022
gb|CA017263.1|CA017263 HV14A15u HV Hordeum vulgare subsp. v... 64 1e-022
gb|BM101447.2|BM101447 EBpi01_SQ003_O01_R pistil, 1 DPA, no... 64 1e-022
gb|CV055940.1|CV055940 BNEL12B5 Barley EST endosperm librar... 60 3e-021
gb|BJ453165.1|BJ453165 BJ453165 K. Sato unpublished cDNA li... 72 3e-021
gb|BQ470849.1|BQ470849 HX04D14r HX Hordeum vulgare subsp. v... 59 4e-021
gb|BU984183.1|BU984183 HF03D17r HF Hordeum vulgare subsp. v... 68 6e-021
>gb|AJ463236.1|AJ463236 AJ463236 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200037A04F1, mRNA sequence
Length = 420
Score = 177 bits (380), Expect = 8e-044
Identities = 72/90 (80%), Positives = 76/90 (84%)
Frame = -3 / +1
Query: 715 YLEVLIEFEDGDGDLSAVHLMEAGCGTWTPMVQNWGALWRYNSNTGKALKAPFSLRLTSD 536
YLEVLIEFED DGDL+ V LMEA CGTWTPMVQNWGAL+R NSNTGK L+ PFSLRLTSD
Sbjct: 1 YLEVLIEFEDDDGDLNTVDLMEANCGTWTPMVQNWGALYRLNSNTGKPLRGPFSLRLTSD 180
Query: 535 SGRVLVANNVIPAGWNPGATYRSLVNYS*L 446
SGR LV NNVIP W GATYRSLVNY *+
Sbjct: 181 SGRKLVVNNVIPVSWKAGATYRSLVNYP*V 270
Score = 115 bits (246), Expect = 3e-025
Identities = 50/65 (76%), Positives = 53/65 (81%)
Frame = +2 / -1
Query: 521 EHPAGVGGQPEREGRLQRLAGVGVVAPERAPVLHHRGPRAAPGLHEVDGAEVAVAVLELD 700
E PAGVGG+PEREG QRLAGVGV A +RAPVLHH P AA G HEVDG EVAV VLELD
Sbjct: 195 ELPAGVGGEPEREGPAQRLAGVGVEAIQRAPVLHHGRPGAAVGFHEVDGVEVAVVVLELD 16
Query: 701 EHLQV 715
+HLQV
Sbjct: 15 QHLQV 1
Score = 115 bits (245), Expect = 3e-025
Identities = 55/73 (75%), Positives = 56/73 (76%)
Frame = +3 / -2
Query: 456 *FTKERYVAPGFQPAGMTLLATSTLPESEVSRSEKGAFSALPVLEL*RQSAPQFCTIGVH 635
* TKER VAP FQ GMTLL TS LPESEVSRSEKG S LPVLEL R SAPQFCT+GV
Sbjct: 260 *LTKER*VAPAFQLTGMTLLTTSFLPESEVSRSEKGPRSGLPVLELRRYSAPQFCTMGVQ 81
Query: 636 VPHPASMRWTALR 674
VP ASMR T LR
Sbjct: 80 VPQLASMRSTVLR 42
>gb|BF257093.3|BF257093 HVSMEf0011N11f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0011N11f, mRNA sequence
Length = 816
Score = 137 bits (293), Expect(2) = 4e-038
Identities = 54/65 (83%), Positives = 58/65 (89%)
Frame = -3 / +2
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCGTWTPMVQNWGALWRYNSNTGKALKAPFSLR 548
SNPFYLEVLIEFED DGDL+ V LMEA CGTWTPMVQNWGAL+R NSNTGK L+ PFSLR
Sbjct: 368 SNPFYLEVLIEFEDDDGDLNTVDLMEANCGTWTPMVQNWGALYRLNSNTGKPLRGPFSLR 547
Query: 547 LTSDS 533
+TSDS
Sbjct: 548 VTSDS 562
Score = 111 bits (238), Expect = 3e-024
Identities = 49/64 (76%), Positives = 52/64 (81%)
Frame = +2 / -3
Query: 533 GVGGQPEREGRLQRLAGVGVVAPERAPVLHHRGPRAAPGLHEVDGAEVAVAVLELDEHLQ 712
GVGG PEREG QRLAGVGV A +RAPVLHH P AA GLHEVDG +VAV VLELD+HLQ
Sbjct: 562 GVGGDPEREGPAQRLAGVGVEAIQRAPVLHHGRPGAAVGLHEVDGVKVAVVVLELDQHLQ 383
Query: 713 VERV 724
VE V
Sbjct: 382 VEGV 371
Score = 40.9 bits (83), Expect(2) = 4e-038
Identities = 16/22 (72%), Positives = 16/22 (72%)
Frame = -3 / +1
Query: 511 NVIPAGWNPGATYRSLVNYS*L 446
NV P W GATYRSLVNY *L
Sbjct: 586 NVFPVSWKAGATYRSLVNYP*L 651
>gb|BI950777.1|BI950777 HVSMEl0023C15f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023C15f, mRNA sequence
Length = 986
Score = 87.2 bits (184), Expect(2) = 3e-035
Identities = 33/47 (70%), Positives = 36/47 (76%)
Frame = -3 / +3
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCGTWTPMVQNWGALWRYNS 587
SNPFY + LIEFED DGDL AV L EAG G WT M Q+WGALWR N+
Sbjct: 318 SNPFYFKTLIEFEDDDGDLKAVALKEAGSGAWTQMTQDWGALWRLNN 458
Score = 81.3 bits (171), Expect(2) = 3e-035
Identities = 34/44 (77%), Positives = 36/44 (81%)
Frame = -3 / +3
Query: 586 NTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNY 455
N G L+APFSL+LTSDSGR LV NNVI A WN GATYRSLVNY
Sbjct: 453 NNGNRLRAPFSLQLTSDSGRKLVINNVILANWNAGATYRSLVNY 584
Score = 73.0 bits (153), Expect(2) = 2e-024
Identities = 35/46 (76%), Positives = 37/46 (80%)
Frame = +3 / -2
Query: 450 QE*FTKERYVAPGFQPAGMTLLATSTLPESEVSRSEKGAFSALPVL 587
Q *FT+ERYVAP FQ A MTLL TS LPESEVS SEKGA S LP+L
Sbjct: 589 QG*FTRERYVAPAFQLARMTLLMTSFLPESEVSWSEKGARSRLPLL 452
Score = 58.8 bits (122), Expect(2) = 2e-024
Identities = 29/45 (64%), Positives = 32/45 (71%)
Frame = +2 / -1
Query: 590 VVAPERAPVLHHRGPRAAPGLHEVDGAEVAVAVLELDEHLQVERV 724
V AP+RAPVL H P AA GL E G EVAV VLELD+ L+VE V
Sbjct: 455 VEAPQRAPVLCHLRPGAAAGLLEGHGLEVAVVVLELDQGLEVEGV 321
>gb|AJ463237.1|AJ463237 AJ463237 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200087A08F1, mRNA sequence
Length = 420
Score = 138 bits (296), Expect = 3e-032
Identities = 55/69 (79%), Positives = 58/69 (84%)
Frame = -3 / +3
Query: 652 EAGCGTWTPMVQNWGALWRYNSNTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATY 473
EA CGTWTPMVQNWGAL+R NSNTGK L+ PFSLRLTSDSGR LV NNVIP W GATY
Sbjct: 3 EANCGTWTPMVQNWGALYRLNSNTGKPLRGPFSLRLTSDSGRKLVVNNVIPVSWKAGATY 182
Query: 472 RSLVNYS*L 446
RSLVNY *+
Sbjct: 183 RSLVNYP*V 209
Score = 105 bits (224), Expect = 3e-022
Identities = 50/66 (75%), Positives = 51/66 (77%)
Frame = +3 / -3
Query: 456 *FTKERYVAPGFQPAGMTLLATSTLPESEVSRSEKGAFSALPVLEL*RQSAPQFCTIGVH 635
* TKER VAP FQ GMTLL TS LPESEVSRSEKG S LPVLEL R SAPQFCT+GV
Sbjct: 199 *LTKER*VAPAFQLTGMTLLTTSFLPESEVSRSEKGPRSGLPVLELRRYSAPQFCTMGVQ 20
Query: 636 VPHPAS 653
VP AS
Sbjct: 19 VPQLAS 2
>gb|BU980984.1|BU980984 HA22E18r HA Hordeum vulgare subsp. vulgare cDNA clone HA22E18
5-PRIME, mRNA sequence
Length = 567
Score = 82.2 bits (173), Expect(3) = 7e-032
Identities = 32/44 (72%), Positives = 37/44 (84%)
Frame = -3 / +3
Query: 586 NTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNY 455
N+G L APFS+RLTS SG+ LVA+NVIPAGW PG TYRS+VNY
Sbjct: 363 NSGPMLHAPFSVRLTSSSGKTLVASNVIPAGWKPGGTYRSVVNY 494
Score = 56.1 bits (116), Expect(3) = 7e-032
Identities = 22/28 (78%), Positives = 25/28 (89%)
Frame = -3 / +3
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAG 644
SNP+YL VLIE+EDGDGDLS+V LME G
Sbjct: 222 SNPYYLAVLIEYEDGDGDLSSVELMENG 305
Score = 36.8 bits (74), Expect(3) = 7e-032
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = -3 / +3
Query: 634 WTPMVQNWGALWRYNS 587
WT M ++WGA+WRYNS
Sbjct: 321 WTKMDRSWGAVWRYNS 368
>gb|BM098205.2|BM098205 EBpi03_SQ002_N14_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ002_N14 5', mRNA sequence
Length = 550
Score = 82.2 bits (173), Expect(3) = 7e-032
Identities = 32/44 (72%), Positives = 37/44 (84%)
Frame = -3 / +1
Query: 586 NTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNY 455
N+G L APFS+RLTS SG+ LVA+NVIPAGW PG TYRS+VNY
Sbjct: 349 NSGPMLHAPFSVRLTSSSGKTLVASNVIPAGWKPGGTYRSVVNY 480
Score = 56.1 bits (116), Expect(3) = 7e-032
Identities = 22/28 (78%), Positives = 25/28 (89%)
Frame = -3 / +1
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAG 644
SNP+YL VLIE+EDGDGDLS+V LME G
Sbjct: 208 SNPYYLAVLIEYEDGDGDLSSVELMENG 291
Score = 36.8 bits (74), Expect(3) = 7e-032
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = -3 / +1
Query: 634 WTPMVQNWGALWRYNS 587
WT M ++WGA+WRYNS
Sbjct: 307 WTKMDRSWGAVWRYNS 354
>gb|CB880652.1|CB880652 HM07E17w HM Hordeum vulgare subsp. vulgare cDNA clone HM07E17
3-PRIME, mRNA sequence
Length = 562
Score = 82.2 bits (173), Expect(2) = 1e-029
Identities = 29/47 (61%), Positives = 37/47 (78%)
Frame = -3 / -2
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCGTWTPMVQNWGALWRYNS 587
SNP Y VL+E+EDGDGD+ V LMEA GTWTPM ++WG++WR +S
Sbjct: 546 SNPVYFAVLVEYEDGDGDVVQVDLMEANSGTWTPMRESWGSIWRLDS 406
Score = 67.5 bits (141), Expect(2) = 1e-029
Identities = 26/46 (56%), Positives = 36/46 (78%)
Frame = -3 / -2
Query: 586 NTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS* 449
++G L+APFS+R+T++SG+ LVA+ VIPA W P YRS+V YS*
Sbjct: 411 DSGHRLQAPFSMRITNESGKTLVADKVIPANWAPSTFYRSIVQYS* 274
>gb|CB881234.1|CB881234 HM09C23w HM Hordeum vulgare subsp. vulgare cDNA clone HM09C23
3-PRIME, mRNA sequence
Length = 563
Score = 82.2 bits (173), Expect(2) = 1e-029
Identities = 29/47 (61%), Positives = 37/47 (78%)
Frame = -3 / -2
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCGTWTPMVQNWGALWRYNS 587
SNP Y VL+E+EDGDGD+ V LMEA GTWTPM ++WG++WR +S
Sbjct: 547 SNPVYFAVLVEYEDGDGDVVQVDLMEANSGTWTPMRESWGSIWRLDS 407
Score = 67.5 bits (141), Expect(2) = 1e-029
Identities = 26/46 (56%), Positives = 36/46 (78%)
Frame = -3 / -2
Query: 586 NTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS* 449
++G L+APFS+R+T++SG+ LVA+ VIPA W P YRS+V YS*
Sbjct: 412 DSGHRLQAPFSMRITNESGKTLVADKVIPANWAPSTFYRSIVQYS* 275
>gb|BQ759881.1|BQ759881 EBpi05_SQ004_P16_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ004_P16 5', mRNA sequence
Length = 500
Score = 82.2 bits (173), Expect(2) = 1e-029
Identities = 29/47 (61%), Positives = 37/47 (78%)
Frame = -3 / +2
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCGTWTPMVQNWGALWRYNS 587
SNP Y VL+E+EDGDGD+ V LMEA GTWTPM ++WG++WR +S
Sbjct: 155 SNPVYFAVLVEYEDGDGDVVQVDLMEANSGTWTPMRESWGSIWRLDS 295
Score = 67.5 bits (141), Expect(2) = 1e-029
Identities = 26/46 (56%), Positives = 36/46 (78%)
Frame = -3 / +2
Query: 586 NTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS* 449
++G L+APFS+R+T++SG+ LVA+ VIPA W P YRS+V YS*
Sbjct: 290 DSGHRLQAPFSMRITNESGKTLVADKVIPANWAPSTFYRSIVQYS* 427
>gb|AV928430.1|AV928430 AV928430 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basdg18 3', mRNA sequence
Length = 704
Score = 72.1 bits (151), Expect(2) = 1e-026
Identities = 29/54 (53%), Positives = 37/54 (68%)
Frame = -3 / -2
Query: 595 YNSNTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS*LMNCG 434
+ ++ L+ PFS+R+TSDSG+ LVANNVIPA W P YRS V +S*L G
Sbjct: 484 WRMDSNHRLQGPFSMRITSDSGKKLVANNVIPANWRPNTDYRSFVQFS*LAPTG 323
Score = 67.5 bits (141), Expect(2) = 1e-026
Identities = 25/48 (52%), Positives = 34/48 (70%)
Frame = -3 / -2
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCGTWTPMVQNWGALWRYNSN 584
SN YL VLIE+ED DGD+ V + EA G+W M ++WG++WR +SN
Sbjct: 610 SNAVYLAVLIEYEDMDGDVIQVDMKEANSGSWMAMRESWGSIWRMDSN 467
>gb|BQ467895.1|BQ467895 HR01E04r HR Hordeum vulgare subsp. vulgare cDNA clone HR01E04
5-PRIME, mRNA sequence
Length = 540
Score = 72.1 bits (151), Expect(2) = 1e-026
Identities = 29/54 (53%), Positives = 37/54 (68%)
Frame = -3 / +2
Query: 595 YNSNTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS*LMNCG 434
+ ++ L+ PFS+R+TSDSG+ LVANNVIPA W P YRS V +S*L G
Sbjct: 143 WRMDSNHRLQGPFSMRITSDSGKKLVANNVIPANWRPNTDYRSFVQFS*LAPTG 304
Score = 67.5 bits (141), Expect(2) = 1e-026
Identities = 25/48 (52%), Positives = 34/48 (70%)
Frame = -3 / +2
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCGTWTPMVQNWGALWRYNSN 584
SN YL VLIE+ED DGD+ V + EA G+W M ++WG++WR +SN
Sbjct: 17 SNAVYLAVLIEYEDMDGDVIQVDMKEANSGSWMAMRESWGSIWRMDSN 160
>gb|BU986289.1|BU986289 HF11E17r HF Hordeum vulgare subsp. vulgare cDNA clone HF11E17
5-PRIME, mRNA sequence
Length = 538
Score = 72.1 bits (151), Expect(2) = 1e-026
Identities = 25/40 (62%), Positives = 33/40 (82%)
Frame = -3 / +2
Query: 706 VLIEFEDGDGDLSAVHLMEAGCGTWTPMVQNWGALWRYNS 587
VL+E+EDGDGD+ V LMEA GTWTPM ++WG++WR +S
Sbjct: 14 VLVEYEDGDGDVVQVDLMEANSGTWTPMRESWGSIWRLDS 133
Score = 67.5 bits (141), Expect(2) = 1e-026
Identities = 26/46 (56%), Positives = 36/46 (78%)
Frame = -3 / +2
Query: 586 NTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS* 449
++G L+APFS+R+T++SG+ LVA+ VIPA W P YRS+V YS*
Sbjct: 128 DSGHRLQAPFSMRITNESGKTLVADKVIPANWAPSTFYRSIVQYS* 265
>gb|BF260026.3|BF260026 HVSMEf0020O13f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020O13f, mRNA sequence
Length = 535
Score = 72.1 bits (151), Expect(2) = 1e-026
Identities = 29/54 (53%), Positives = 37/54 (68%)
Frame = -3 / +1
Query: 595 YNSNTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS*LMNCG 434
+ ++ L+ PFS+R+TSDSG+ LVANNVIPA W P YRS V +S*L G
Sbjct: 262 WRMDSNHRLQGPFSMRITSDSGKKLVANNVIPANWRPNTDYRSFVQFS*LAPTG 423
Score = 67.5 bits (141), Expect(2) = 1e-026
Identities = 25/48 (52%), Positives = 34/48 (70%)
Frame = -3 / +1
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCGTWTPMVQNWGALWRYNSN 584
SN YL VLIE+ED DGD+ V + EA G+W M ++WG++WR +SN
Sbjct: 136 SNAVYLAVLIEYEDMDGDVIQVDMKEANSGSWMAMRESWGSIWRMDSN 279
>gb|CA019924.1|CA019924 HV13L18r HV Hordeum vulgare subsp. vulgare cDNA clone HV13L18
5-PRIME, mRNA sequence
Length = 549
Score = 116 bits (247), Expect = 2e-025
Identities = 45/54 (83%), Positives = 48/54 (88%)
Frame = -3 / +3
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCGTWTPMVQNWGALWRYNSNTGKALK 566
SNPFYLEVLIEFED DGDL+ V LMEA CGTWTPMVQNWGAL+R NSNTGK L+
Sbjct: 384 SNPFYLEVLIEFEDDDGDLNTVDLMEANCGTWTPMVQNWGALYRLNSNTGKPLR 545
>gb|BJ463021.1|BJ463021 BJ463021 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags25g15 5', mRNA sequence
Length = 532
Score = 87.2 bits (184), Expect(2) = 2e-025
Identities = 33/47 (70%), Positives = 36/47 (76%)
Frame = -3 / +3
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCGTWTPMVQNWGALWRYNS 587
SNPFY + LIEFED DGDL AV L EAG G WT M Q+WGALWR N+
Sbjct: 312 SNPFYFKTLIEFEDDDGDLKAVALKEAGSGAWTQMTQDWGALWRLNN 452
Score = 48.3 bits (99), Expect(2) = 2e-025
Identities = 20/27 (74%), Positives = 21/27 (77%)
Frame = -3 / +3
Query: 586 NTGKALKAPFSLRLTSDSGRVLVANNV 506
N G L+APFSLRLT DSGR LV NNV
Sbjct: 447 NNGNRLRAPFSLRLTXDSGRKLVXNNV 527
>gb|BG369116.2|BG369116 HVSMEi0021M10f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0021M10f, mRNA sequence
Length = 609
Score = 54.7 bits (113), Expect(3) = 3e-023
Identities = 23/34 (67%), Positives = 27/34 (79%)
Frame = -3 / +1
Query: 586 NTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNP 485
N+G L APFS+RLTS SG+ LVA+NVIPAG P
Sbjct: 322 NSGPMLHAPFSVRLTSSSGKTLVASNVIPAG*KP 423
Score = 54.2 bits (112), Expect(3) = 3e-023
Identities = 21/27 (77%), Positives = 24/27 (88%)
Frame = -3 / +1
Query: 724 NPFYLEVLIEFEDGDGDLSAVHLMEAG 644
NP+YL VLIE+EDGDGDLS+V LME G
Sbjct: 184 NPYYLAVLIEYEDGDGDLSSVELMENG 264
Score = 36.8 bits (74), Expect(3) = 3e-023
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = -3 / +1
Query: 634 WTPMVQNWGALWRYNS 587
WT M ++WGA+WRYNS
Sbjct: 280 WTKMDRSWGAVWRYNS 327
>gb|CK124171.1|CK124171 BES1824107e17 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010E177 5-PRIME, mRNA sequence
Length = 751
Score = 64.3 bits (134), Expect(3) = 4e-023
Identities = 25/41 (60%), Positives = 31/41 (75%)
Frame = -3 / +1
Query: 571 LKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS* 449
L+APFS+R+ ++SG+ LVAN VIPA W P YRS V YS*
Sbjct: 232 LQAPFSIRIRNESGKTLVANKVIPANWRPNTFYRSFVQYS* 354
Score = 45.1 bits (92), Expect(3) = 4e-023
Identities = 17/27 (62%), Positives = 21/27 (77%)
Frame = -3 / +1
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEA 647
SNP Y VL+E+EDGDGD+ V LME+
Sbjct: 73 SNPVYFAVLVEYEDGDGDVVQVDLMES 153
Score = 35.9 bits (72), Expect(3) = 4e-023
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = -3 / +1
Query: 646 GCGTWTPMVQNWGALWRYNSN 584
G G WT M ++WG++WR +SN
Sbjct: 163 GGGKWTRMRESWGSVWRLDSN 225
>gb|CK122346.1|CK122346 BES1824102p12 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P122 5-PRIME, mRNA sequence
Length = 717
Score = 64.3 bits (134), Expect(3) = 4e-023
Identities = 25/41 (60%), Positives = 31/41 (75%)
Frame = -3 / +1
Query: 571 LKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS* 449
L+APFS+R+ ++SG+ LVAN VIPA W P YRS V YS*
Sbjct: 469 LQAPFSIRIRNESGKTLVANKVIPANWRPNTFYRSFVQYS* 591
Score = 45.1 bits (92), Expect(3) = 4e-023
Identities = 17/27 (62%), Positives = 21/27 (77%)
Frame = -3 / +1
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEA 647
SNP Y VL+E+EDGDGD+ V LME+
Sbjct: 310 SNPVYFAVLVEYEDGDGDVVQVDLMES 390
Score = 35.9 bits (72), Expect(3) = 4e-023
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = -3 / +1
Query: 646 GCGTWTPMVQNWGALWRYNSN 584
G G WT M ++WG++WR +SN
Sbjct: 400 GGGKWTRMRESWGSVWRLDSN 462
>gb|BE060246.3|BE060246 HVSMEg0011L08f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011L08f, mRNA sequence
Length = 544
Score = 64.3 bits (134), Expect(3) = 4e-023
Identities = 25/41 (60%), Positives = 31/41 (75%)
Frame = -3 / +2
Query: 571 LKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS* 449
L+APFS+R+ ++SG+ LVAN VIPA W P YRS V YS*
Sbjct: 341 LQAPFSIRIRNESGKTLVANKVIPANWRPNTFYRSFVQYS* 463
Score = 45.1 bits (92), Expect(3) = 4e-023
Identities = 17/27 (62%), Positives = 21/27 (77%)
Frame = -3 / +2
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEA 647
SNP Y VL+E+EDGDGD+ V LME+
Sbjct: 182 SNPVYFAVLVEYEDGDGDVVQVDLMES 262
Score = 35.9 bits (72), Expect(3) = 4e-023
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = -3 / +2
Query: 646 GCGTWTPMVQNWGALWRYNSN 584
G G WT M ++WG++WR +SN
Sbjct: 272 GGGKWTRMRESWGSVWRLDSN 334
>gb|BQ459628.1|BQ459628 HA08J01r HA Hordeum vulgare subsp. vulgare cDNA clone HA08J01
5-PRIME, mRNA sequence
Length = 470
Score = 64.3 bits (134), Expect(3) = 4e-023
Identities = 25/41 (60%), Positives = 31/41 (75%)
Frame = -3 / +1
Query: 571 LKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS* 449
L+APFS+R+ ++SG+ LVAN VIPA W P YRS V YS*
Sbjct: 301 LQAPFSIRIRNESGKTLVANKVIPANWRPNTFYRSFVQYS* 423
Score = 45.1 bits (92), Expect(3) = 4e-023
Identities = 17/27 (62%), Positives = 21/27 (77%)
Frame = -3 / +1
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEA 647
SNP Y VL+E+EDGDGD+ V LME+
Sbjct: 142 SNPVYFAVLVEYEDGDGDVVQVDLMES 222
Score = 35.9 bits (72), Expect(3) = 4e-023
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = -3 / +1
Query: 646 GCGTWTPMVQNWGALWRYNSN 584
G G WT M ++WG++WR +SN
Sbjct: 232 GGGKWTRMRESWGSVWRLDSN 294
>gb|AV917139.1|AV917139 AV917139 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20o17 5', mRNA sequence
Length = 590
Score = 63.8 bits (133), Expect(3) = 1e-022
Identities = 24/42 (57%), Positives = 32/42 (76%)
Frame = -3 / +1
Query: 571 LKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS*L 446
L+APFS+R+ ++SG+ LVA NVIP W P YRS+V YS*+
Sbjct: 397 LQAPFSIRIRNESGKTLVARNVIPKNWRPNTFYRSIVQYS*I 522
Score = 43.7 bits (89), Expect(3) = 1e-022
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = -3 / +1
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCG 638
SN Y+ +L+E+E+GDGD+ V LME+G G
Sbjct: 232 SNAVYMAILVEYENGDGDVVQVDLMESGRG 321
Score = 36.3 bits (73), Expect(3) = 1e-022
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = -3 / +1
Query: 646 GCGTWTPMVQNWGALWRYNSN 584
G G WT M ++WG++WR +SN
Sbjct: 328 GGGRWTRMRESWGSIWRLDSN 390
>gb|CA017263.1|CA017263 HV14A15u HV Hordeum vulgare subsp. vulgare cDNA clone HV14A15
3-PRIME, mRNA sequence
Length = 569
Score = 63.8 bits (133), Expect(3) = 1e-022
Identities = 24/42 (57%), Positives = 32/42 (76%)
Frame = -3 / -2
Query: 571 LKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS*L 446
L+APFS+R+ ++SG+ LVA NVIP W P YRS+V YS*+
Sbjct: 325 LQAPFSIRIRNESGKTLVARNVIPKNWRPNTFYRSIVQYS*I 200
Score = 43.7 bits (89), Expect(3) = 1e-022
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = -3 / -2
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCG 638
SN Y+ +L+E+E+GDGD+ V LME+G G
Sbjct: 490 SNAVYMAILVEYENGDGDVVQVDLMESGRG 401
Score = 36.3 bits (73), Expect(3) = 1e-022
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = -3 / -2
Query: 646 GCGTWTPMVQNWGALWRYNSN 584
G G WT M ++WG++WR +SN
Sbjct: 394 GGGRWTRMRESWGSIWRLDSN 332
>gb|BM101447.2|BM101447 EBpi01_SQ003_O01_R pistil, 1 DPA, no treatment, cv Optic, EBpi01
Hordeum vulgare subsp. vulgare cDNA clone
EBpi01_SQ003_O01 5', mRNA sequence
Length = 452
Score = 63.8 bits (133), Expect(3) = 1e-022
Identities = 24/42 (57%), Positives = 32/42 (76%)
Frame = -3 / +1
Query: 571 LKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS*L 446
L+APFS+R+ ++SG+ LVA NVIP W P YRS+V YS*+
Sbjct: 217 LQAPFSIRIRNESGKTLVARNVIPKNWRPNTFYRSIVQYS*I 342
Score = 43.7 bits (89), Expect(3) = 1e-022
Identities = 16/30 (53%), Positives = 23/30 (76%)
Frame = -3 / +1
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCG 638
SN Y+ +L+E+E+GDGD+ V LME+G G
Sbjct: 52 SNAVYMAILVEYENGDGDVVQVDLMESGRG 141
Score = 36.3 bits (73), Expect(3) = 1e-022
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = -3 / +1
Query: 646 GCGTWTPMVQNWGALWRYNSN 584
G G WT M ++WG++WR +SN
Sbjct: 148 GGGRWTRMRESWGSIWRLDSN 210
>gb|CV055940.1|CV055940 BNEL12B5 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL12B5 5' similar to Oryza sativa
beta-expansin, mRNA sequence
Length = 614
Score = 59.7 bits (124), Expect(3) = 3e-021
Identities = 23/44 (52%), Positives = 32/44 (72%)
Frame = -3 / -2
Query: 571 LKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS*LMN 440
+ PFSLR+T++SG+ LVA+ VIP W P TY SLV + *L++
Sbjct: 292 MHGPFSLRVTNESGKTLVADQVIPGDWKPNKTYSSLVQFH*LID 161
Score = 45.5 bits (93), Expect(3) = 3e-021
Identities = 15/30 (50%), Positives = 24/30 (80%)
Frame = -3 / -2
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEAGCG 638
SNPFY+ +L+E+E+GDGD+ + +ME+ G
Sbjct: 475 SNPFYMAILVEYENGDGDVKQLDIMESRPG 386
Score = 33.6 bits (67), Expect(3) = 3e-021
Identities = 8/19 (42%), Positives = 15/19 (78%)
Frame = -3 / -2
Query: 640 GTWTPMVQNWGALWRYNSN 584
G W PM ++WG++WR +++
Sbjct: 355 GQWVPMKESWGSIWRMDAH 299
>gb|BJ453165.1|BJ453165 BJ453165 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41p01 5', mRNA sequence
Length = 608
Score = 72.1 bits (151), Expect(2) = 3e-021
Identities = 29/54 (53%), Positives = 37/54 (68%)
Frame = -3 / +1
Query: 595 YNSNTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS*LMNCG 434
+ ++ L+ PFS+R+TSDSG+ LVANNVIPA W P YRS V +S*L G
Sbjct: 103 WRMDSNHRLQGPFSMRITSDSGKKLVANNVIPANWRPNTDYRSFVQFS*LAPTG 264
Score = 49.2 bits (101), Expect(2) = 3e-021
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = -3 / +1
Query: 685 GDGDLSAVHLMEAGCGTWTPMVQNWGALWRYNSN 584
GDGD+ V + EA G+W M ++WG++WR +SN
Sbjct: 19 GDGDVIQVDMKEANSGSWMAMRESWGSIWRMDSN 120
>gb|BQ470849.1|BQ470849 HX04D14r HX Hordeum vulgare subsp. vulgare cDNA clone HX04D14
5-PRIME, mRNA sequence
Length = 606
Score = 59.3 bits (123), Expect(3) = 4e-021
Identities = 21/49 (42%), Positives = 34/49 (69%)
Frame = -3 / +2
Query: 595 YNSNTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS* 449
+ +T + ++ PFSLR+T++SG+ LVA+ VIPA W P Y S++ + *
Sbjct: 221 WRMDTRRPMQGPFSLRITNESGKTLVADQVIPADWEPNEIYSSIIQFD* 367
Score = 44.6 bits (91), Expect(3) = 4e-021
Identities = 14/27 (51%), Positives = 23/27 (85%)
Frame = -3 / +2
Query: 727 SNPFYLEVLIEFEDGDGDLSAVHLMEA 647
SNP+YL +L+E+E+GDGD+ V +M++
Sbjct: 59 SNPYYLAILVEYENGDGDVDQVDMMQS 139
Score = 34.5 bits (69), Expect(3) = 4e-021
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = -3 / +2
Query: 649 AGCGTWTPMVQNWGALWRYNS 587
A G W PM ++WG++WR ++
Sbjct: 173 APTGEWVPMTESWGSIWRMDT 235
>gb|BU984183.1|BU984183 HF03D17r HF Hordeum vulgare subsp. vulgare cDNA clone HF03D17
5-PRIME, mRNA sequence
Length = 386
Score = 67.5 bits (141), Expect(2) = 6e-021
Identities = 26/46 (56%), Positives = 36/46 (78%)
Frame = -3 / +2
Query: 586 NTGKALKAPFSLRLTSDSGRVLVANNVIPAGWNPGATYRSLVNYS* 449
++G L+APFS+R+T++SG+ LVA+ VIPA W P YRS+V YS*
Sbjct: 89 DSGHRLQAPFSMRITNESGKTLVADKVIPANWAPSTFYRSIVQYS* 226
Score = 52.8 bits (109), Expect(2) = 6e-021
Identities = 18/31 (58%), Positives = 24/31 (77%)
Frame = -3 / +2
Query: 679 GDLSAVHLMEAGCGTWTPMVQNWGALWRYNS 587
GD+ V LMEA GTWTPM ++WG++WR +S
Sbjct: 2 GDVVQVDLMEANSGTWTPMRESWGSIWRLDS 94
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 300,607,104
Number of Sequences: 312970
Number of extensions: 4624195
Number of successful extensions: 48431
Number of sequences better than 1.0e-020: 34
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8101591419
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)