TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2478290.2.1
(1343 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CK125116.1|CK125116 BES1824106l24 BES1824 Hordeum vulgar... 199 2e-080
gb|AL504252.1|AL504252 AL504252 Hordeum vulgare Barke roots... 196 7e-079
gb|BJ453869.1|BJ453869 BJ453869 K. Sato unpublished cDNA li... 199 3e-078
gb|AL504719.1|AL504719 AL504719 Hordeum vulgare Barke roots... 149 1e-072
gb|BJ461404.1|BJ461404 BJ461404 K. Sato unpublished cDNA li... 188 4e-047
gb|BI778941.2|BI778941 EBro01_SQ002_C23_R root, 3 week, hyd... 128 2e-043
gb|CA012046.1|CA012046 HT04E15r HT Hordeum vulgare subsp. v... 109 1e-036
gb|BQ766925.1|BQ766925 EBro08_SQ007_B17_R root, 3 week, dro... 98 4e-033
gb|CA008912.1|CA008912 HU12I03r HU Hordeum vulgare subsp. v... 131 7e-033
gb|BQ762675.1|BQ762675 EBro02_SQ004_O15 _R root, 3 week, hy... 94 6e-032
gb|AW982401.2|AW982401 HVSMEg0003C19f Hordeum vulgare pre-a... 135 4e-031
gb|BQ465184.1|BQ465184 HU02O05r HU Hordeum vulgare subsp. v... 131 8e-030
gb|BI947127.1|BI947127 HVSMEl0003N07f Hordeum vulgare spike... 116 2e-029
gb|BF064456.3|BF064456 HV_CEb0015C20f Hordeum vulgare seedl... 124 1e-027
gb|BQ663493.1|BQ663493 HU02O05u HU Hordeum vulgare subsp. v... 120 2e-026
gb|AL501583.1|AL501583 AL501583 Hordeum vulgare Barke roots... 98 1e-021
gb|BG344635.2|BG344635 HVSMEg0009O02f Hordeum vulgare pre-a... 63 2e-021
>gb|CK125116.1|CK125116 BES1824106l24 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010L246 5-PRIME, mRNA sequence
Length = 777
Score = 199 bits (429), Expect(2) = 2e-080
Identities = 83/104 (79%), Positives = 90/104 (86%)
Frame = +1 / +1
Query: 433 DVAVNLIITDYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINRCLEEGADEFF 612
+V VNLIITDYCMPGMTGYDLLKKIKESSS RDIPVVIMSSENIPSRINRCLEEGA+EFF
Sbjct: 316 EVNVNLIITDYCMPGMTGYDLLKKIKESSSFRDIPVVIMSSENIPSRINRCLEEGANEFF 495
Query: 613 LKPVRLSDMNKLKPHILKSRCNQEQHQQSDSHSGERRNPTISSS 744
LKPVRLSDM+KLKPHI+KSRC + H+ D S NPT +SS
Sbjct: 496 LKPVRLSDMSKLKPHIMKSRCKEHSHEDQDLLSNSDTNPTNNSS 627
Score = 155 bits (332), Expect(3) = 6e-063
Identities = 65/74 (87%), Positives = 71/74 (95%)
Frame = -1 / -2
Query: 665 FSMWGLSLFMSDSRTGFRKNSSAPSSRHLLILEGMFSEDMITTGISLRDDDSLIFLSRSY 486
F +WGLSL MSDSRTGFRKNS APSSRHLLILEGMFS+DMITTG+SL+DDDSLIFLSRSY
Sbjct: 548 FIIWGLSLLMSDSRTGFRKNSFAPSSRHLLILEGMFSDDMITTGMSLKDDDSLIFLSRSY 369
Query: 485 PVMPGMQ*SVMIRF 444
PV+PGMQ*SVM+RF
Sbjct: 368 PVIPGMQ*SVMMRF 327
Score = 134 bits (288), Expect(3) = 1e-044
Identities = 59/101 (58%), Positives = 72/101 (71%)
Frame = +3 / +3
Query: 444 EPDHHRLLHAWHDRI*PAQEDQGVVVSQRYPGGDHVL*EHSFKDQ*VPGGRS*RVLPKTC 623
EP HH LLHAW DRI AQEDQGVVV Q +PG DHV+ EH+F++ +PGGRS VLP+T
Sbjct: 327 EPHHH*LLHAWDDRIRSAQEDQGVVVFQGHPGCDHVIGEHTFQN*QMPGGRSK*VLPETS 506
Query: 624 AAIRHEQAQAPHTEKQMQPGTAPAK*QSQWRTQEPHNQQQR 746
AIRHEQAQAP+ EKQMQ ++ + +PH QQQ+
Sbjct: 507 TAIRHEQAQAPYNEKQMQGALPRGPRPAKQQRYQPHKQQQQ 629
Score = 126 bits (270), Expect(2) = 2e-051
Identities = 52/76 (68%), Positives = 63/76 (82%)
Frame = -3 / -1
Query: 660 YVGLELVHV**PHRF*EELVSSFLQAPIDP*RNVLRGHDHHRDISERRRLLDLLEQVISC 481
Y+GLEL HV**P+ F EEL+ SFLQA ++ R VLR HDH+RD+ ERRRLLDLLEQ++SC
Sbjct: 543 YMGLELAHV**PYWFQEELICSFLQASVNSGRYVLR*HDHNRDVPERRRLLDLLEQIVSC 364
Query: 480 HARHAVVCDDQVHSHI 433
H RHAVV DD+VH H+
Sbjct: 363 HPRHAVVSDDEVHVHL 316
Score = 121 bits (258), Expect(2) = 2e-080
Identities = 52/82 (63%), Positives = 63/82 (76%)
Frame = +1 / +1
Query: 199 ALFRSVMAAVATETPFHVLAVDDSLPDRKLIERLLKTSSFQVTTVDSGSKALQFLGLHDQ 378
+L + A E PFHVLAVDDS+ DRKLIERLLKTSSFQVTTVDSG+KALQFLGLH +
Sbjct: 94 SLSSPMAVAAIAEAPFHVLAVDDSVLDRKLIERLLKTSSFQVTTVDSGTKALQFLGLHAE 273
Query: 379 DSTVPPVHTHQLDVAANQDVAV 444
D+++ VH QLDV N ++ +
Sbjct: 274 DASISSVHADQLDVEVNVNLII 339
Score = 105 bits (223), Expect(3) = 6e-063
Identities = 48/88 (54%), Positives = 58/88 (65%)
Frame = -3 / -1
Query: 417 IQLVRVDRRNRAVLVMEAQELQRLAPGVDSGHLEGRGLEEPLDELPVREAVVHRQDMERS 238
+QLVR+DR N VL ME QEL+ L P + SG+LE R LEEPLDELPV++AV+H QDMERS
Sbjct: 312 VQLVRMDRGNGGVLGMEPQELESLGPRIHSGNLERRCLEEPLDELPVKDAVIHGQDMERS 133
Query: 237 LCGHRRHDRSKQGSSRAAGKNWGCRGRK 154
LC R R + + GC RK
Sbjct: 132 LCDRRHRHRRR*RACVLCDGGGGC*RRK 49
Score = 97.3 bits (206), Expect(2) = 2e-051
Identities = 40/63 (63%), Positives = 45/63 (71%)
Frame = -1 / -2
Query: 410 WCVWTGGTVLSWSWRPRNCSALLPESTVVTWKEEVLRSLSMSFLSGRLSSTARTWNGVSV 231
W WT S +W P+N AL+PESTVVTW E+VLRSLSMSFLS LSSTA+TWNG S
Sbjct: 305 WSAWTEEMEASSAWSPKNWRALVPESTVVTWNEDVLRSLSMSFLSRTLSSTAKTWNGASA 126
Query: 230 ATA 222
A
Sbjct: 125 IAA 117
Score = 89.0 bits (188), Expect(3) = 6e-028
Identities = 44/64 (68%), Positives = 46/64 (71%)
Frame = -2 / -3
Query: 676 CICFSVCGA*ACSCLIAAQVLGRTRQLLPPGTY*SLKECSQRT*SPPGYL*ETTTP*SS* 497
CICFS+ GA*ACSCLIA V GRT LLPPG * K CS T*S PG *+TTTP*SS*
Sbjct: 559 CICFSLYGA*ACSCLIAVLVSGRTHLLLPPGIC*FWKVCSPMT*SQPGCP*KTTTP*SS* 380
Query: 496 AGHI 485
A I
Sbjct: 379 ADRI 368
Score = 46.4 bits (95), Expect(3) = 6e-028
Identities = 15/23 (65%), Positives = 20/23 (86%)
Frame = -1 / -1
Query: 152 LEEVISCHARHAVVCDDQVHSHI 84
LE+++SCH RHAVV DD+VH H+
Sbjct: 384 LEQIVSCHPRHAVVSDDEVHVHL 316
Score = 46.4 bits (95), Expect(3) = 1e-044
Identities = 17/26 (65%), Positives = 21/26 (80%)
Frame = +3 / +3
Query: 282 EAHREAPQDLFLPSDHCRLREQGAAV 359
EAHREAPQD+F+PS HC +QG+ V
Sbjct: 177 EAHREAPQDIFVPSYHCGFWDQGSPV 254
Score = 37.7 bits (76), Expect(3) = 1e-044
Identities = 16/25 (64%), Positives = 16/25 (64%)
Frame = +2 / +2
Query: 341 GARRCSSWASMTRTARFLLSTRTSW 415
G R SSW SM RT F LS RTSW
Sbjct: 236 GPRLSSSWGSMPRTPPFPLSMRTSW 310
Score = 32.7 bits (65), Expect(3) = 6e-028
Identities = 14/22 (63%), Positives = 17/22 (77%)
Frame = -1 / -1
Query: 68 IQLVRVDRRNRAVLVMEAQELQ 3
+QLVR+DR N VL ME QEL+
Sbjct: 312 VQLVRMDRGNGGVLGMEPQELE 247
Score = 26.3 bits (51), Expect(3) = 6e-063
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = -2 / -2
Query: 784 RCLHPFSCGCLCYRCC 737
RC +P+ C YRCC
Sbjct: 764 RCCYPWGYCC*WYRCC 717
>gb|AL504252.1|AL504252 AL504252 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW04L02V 5', mRNA sequence
Length = 700
Score = 196 bits (423), Expect(2) = 7e-079
Identities = 81/100 (81%), Positives = 87/100 (87%)
Frame = +1 / +3
Query: 433 DVAVNLIITDYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINRCLEEGADEFF 612
+V VNLIITDYCMPGMTGYDLLKKIKESSS RDIPVVIMSSENIPSRINRCLEEGA+EFF
Sbjct: 333 EVNVNLIITDYCMPGMTGYDLLKKIKESSSFRDIPVVIMSSENIPSRINRCLEEGANEFF 512
Query: 613 LKPVRLSDMNKLKPHILKSRCNQEQHQQSDSHSGERRNPT 732
LKPVRLSDM+KLKPHI+KSRC + H+ D S NPT
Sbjct: 513 LKPVRLSDMSKLKPHIMKSRCKEHSHEDQDLLSNSDTNPT 632
Score = 155 bits (332), Expect(2) = 3e-062
Identities = 65/74 (87%), Positives = 71/74 (95%)
Frame = -1 / -1
Query: 665 FSMWGLSLFMSDSRTGFRKNSSAPSSRHLLILEGMFSEDMITTGISLRDDDSLIFLSRSY 486
F +WGLSL MSDSRTGFRKNS APSSRHLLILEGMFS+DMITTG+SL+DDDSLIFLSRSY
Sbjct: 565 FIIWGLSLLMSDSRTGFRKNSFAPSSRHLLILEGMFSDDMITTGMSLKDDDSLIFLSRSY 386
Query: 485 PVMPGMQ*SVMIRF 444
PV+PGMQ*SVM+RF
Sbjct: 385 PVIPGMQ*SVMMRF 344
Score = 132 bits (282), Expect(3) = 3e-042
Identities = 58/99 (58%), Positives = 70/99 (70%)
Frame = +3 / +2
Query: 444 EPDHHRLLHAWHDRI*PAQEDQGVVVSQRYPGGDHVL*EHSFKDQ*VPGGRS*RVLPKTC 623
EP HH LLHAW DRI AQEDQGVVV Q +PG DHV+ EH+F++ +PGGRS VLP+T
Sbjct: 344 EPHHH*LLHAWDDRIRSAQEDQGVVVFQGHPGCDHVIGEHTFQN*QMPGGRSK*VLPETS 523
Query: 624 AAIRHEQAQAPHTEKQMQPGTAPAK*QSQWRTQEPHNQQ 740
AIRHEQAQAP+ EKQMQ ++ + +PH QQ
Sbjct: 524 TAIRHEQAQAPYNEKQMQGALPRGPRPAKQQRYQPHKQQ 640
Score = 126 bits (270), Expect(2) = 9e-051
Identities = 52/76 (68%), Positives = 63/76 (82%)
Frame = -3 / -3
Query: 660 YVGLELVHV**PHRF*EELVSSFLQAPIDP*RNVLRGHDHHRDISERRRLLDLLEQVISC 481
Y+GLEL HV**P+ F EEL+ SFLQA ++ R VLR HDH+RD+ ERRRLLDLLEQ++SC
Sbjct: 560 YMGLELAHV**PYWFQEELICSFLQASVNSGRYVLR*HDHNRDVPERRRLLDLLEQIVSC 381
Query: 480 HARHAVVCDDQVHSHI 433
H RHAVV DD+VH H+
Sbjct: 380 HPRHAVVSDDEVHVHL 333
Score = 118 bits (253), Expect(2) = 7e-079
Identities = 51/82 (62%), Positives = 62/82 (75%)
Frame = +1 / +3
Query: 199 ALFRSVMAAVATETPFHVLAVDDSLPDRKLIERLLKTSSFQVTTVDSGSKALQFLGLHDQ 378
+L + A E PFHVLAVDDS+ DRKLIERLLKTSSFQVTT DSG+KALQFLGLH +
Sbjct: 111 SLSSPMAVAAIAEAPFHVLAVDDSVLDRKLIERLLKTSSFQVTTXDSGTKALQFLGLHAE 290
Query: 379 DSTVPPVHTHQLDVAANQDVAV 444
D+++ VH QLDV N ++ +
Sbjct: 291 DASISSVHADQLDVEVNVNLII 356
Score = 105 bits (223), Expect(2) = 3e-062
Identities = 48/88 (54%), Positives = 58/88 (65%)
Frame = -3 / -3
Query: 417 IQLVRVDRRNRAVLVMEAQELQRLAPGVDSGHLEGRGLEEPLDELPVREAVVHRQDMERS 238
+QLVR+DR N VL ME QEL+ L P + SG+LE R LEEPLDELPV++AV+H QDMERS
Sbjct: 329 VQLVRMDRGNGGVLGMEPQELESLGPRIXSGNLERRCLEEPLDELPVKDAVIHGQDMERS 150
Query: 237 LCGHRRHDRSKQGSSRAAGKNWGCRGRK 154
LC R R + + GC RK
Sbjct: 149 LCDRRHRHRRR*RACVLCDGGGGC*RRK 66
Score = 95.0 bits (201), Expect(2) = 9e-051
Identities = 39/63 (61%), Positives = 44/63 (69%)
Frame = -1 / -1
Query: 410 WCVWTGGTVLSWSWRPRNCSALLPESTVVTWKEEVLRSLSMSFLSGRLSSTARTWNGVSV 231
W WT S +W P+N AL+PES VVTW E+VLRSLSMSFLS LSSTA+TWNG S
Sbjct: 322 WSAWTEEMEASSAWSPKNWRALVPESXVVTWNEDVLRSLSMSFLSRTLSSTAKTWNGASA 143
Query: 230 ATA 222
A
Sbjct: 142 IAA 134
Score = 89.0 bits (188), Expect(3) = 4e-028
Identities = 44/64 (68%), Positives = 46/64 (71%)
Frame = -2 / -2
Query: 676 CICFSVCGA*ACSCLIAAQVLGRTRQLLPPGTY*SLKECSQRT*SPPGYL*ETTTP*SS* 497
CICFS+ GA*ACSCLIA V GRT LLPPG * K CS T*S PG *+TTTP*SS*
Sbjct: 576 CICFSLYGA*ACSCLIAVLVSGRTHLLLPPGIC*FWKVCSPMT*SQPGCP*KTTTP*SS* 397
Query: 496 AGHI 485
A I
Sbjct: 396 ADRI 385
Score = 46.4 bits (95), Expect(3) = 4e-028
Identities = 15/23 (65%), Positives = 20/23 (86%)
Frame = -1 / -3
Query: 152 LEEVISCHARHAVVCDDQVHSHI 84
LE+++SCH RHAVV DD+VH H+
Sbjct: 401 LEQIVSCHPRHAVVSDDEVHVHL 333
Score = 41.4 bits (84), Expect(3) = 3e-042
Identities = 16/26 (61%), Positives = 20/26 (76%)
Frame = +3 / +2
Query: 282 EAHREAPQDLFLPSDHCRLREQGAAV 359
EAHREAPQD+F+PS H +QG+ V
Sbjct: 194 EAHREAPQDIFVPSYHXGFWDQGSPV 271
Score = 37.7 bits (76), Expect(3) = 3e-042
Identities = 16/25 (64%), Positives = 16/25 (64%)
Frame = +2 / +1
Query: 341 GARRCSSWASMTRTARFLLSTRTSW 415
G R SSW SM RT F LS RTSW
Sbjct: 253 GPRLSSSWGSMPRTPPFPLSMRTSW 327
Score = 32.7 bits (65), Expect(3) = 4e-028
Identities = 14/22 (63%), Positives = 17/22 (77%)
Frame = -1 / -3
Query: 68 IQLVRVDRRNRAVLVMEAQELQ 3
+QLVR+DR N VL ME QEL+
Sbjct: 329 VQLVRMDRGNGGVLGMEPQELE 264
>gb|BJ453869.1|BJ453869 BJ453869 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak44m13 5', mRNA sequence
Length = 641
Score = 199 bits (429), Expect(2) = 3e-078
Identities = 83/104 (79%), Positives = 90/104 (86%)
Frame = +1 / +2
Query: 433 DVAVNLIITDYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINRCLEEGADEFF 612
+V VNLIITDYCMPGMTGYDLLKKIKESSS RDIPVVIMSSENIPSRINRCLEEGA+EFF
Sbjct: 179 EVNVNLIITDYCMPGMTGYDLLKKIKESSSFRDIPVVIMSSENIPSRINRCLEEGANEFF 358
Query: 613 LKPVRLSDMNKLKPHILKSRCNQEQHQQSDSHSGERRNPTISSS 744
LKPVRLSDM+KLKPHI+KSRC + H+ D S NPT +SS
Sbjct: 359 LKPVRLSDMSKLKPHIMKSRCKEHSHEDQDLLSNSDTNPTNNSS 490
Score = 155 bits (332), Expect(3) = 5e-059
Identities = 65/74 (87%), Positives = 71/74 (95%)
Frame = -1 / -3
Query: 665 FSMWGLSLFMSDSRTGFRKNSSAPSSRHLLILEGMFSEDMITTGISLRDDDSLIFLSRSY 486
F +WGLSL MSDSRTGFRKNS APSSRHLLILEGMFS+DMITTG+SL+DDDSLIFLSRSY
Sbjct: 411 FIIWGLSLLMSDSRTGFRKNSFAPSSRHLLILEGMFSDDMITTGMSLKDDDSLIFLSRSY 232
Query: 485 PVMPGMQ*SVMIRF 444
PV+PGMQ*SVM+RF
Sbjct: 231 PVIPGMQ*SVMMRF 190
Score = 134 bits (288), Expect(3) = 1e-044
Identities = 59/101 (58%), Positives = 72/101 (71%)
Frame = +3 / +1
Query: 444 EPDHHRLLHAWHDRI*PAQEDQGVVVSQRYPGGDHVL*EHSFKDQ*VPGGRS*RVLPKTC 623
EP HH LLHAW DRI AQEDQGVVV Q +PG DHV+ EH+F++ +PGGRS VLP+T
Sbjct: 190 EPHHH*LLHAWDDRIRSAQEDQGVVVFQGHPGCDHVIGEHTFQN*QMPGGRSK*VLPETS 369
Query: 624 AAIRHEQAQAPHTEKQMQPGTAPAK*QSQWRTQEPHNQQQR 746
AIRHEQAQAP+ EKQMQ ++ + +PH QQQ+
Sbjct: 370 TAIRHEQAQAPYNEKQMQGALPRGPRPAKQQRYQPHKQQQQ 492
Score = 126 bits (270), Expect(3) = 2e-049
Identities = 52/76 (68%), Positives = 63/76 (82%)
Frame = -3 / -2
Query: 660 YVGLELVHV**PHRF*EELVSSFLQAPIDP*RNVLRGHDHHRDISERRRLLDLLEQVISC 481
Y+GLEL HV**P+ F EEL+ SFLQA ++ R VLR HDH+RD+ ERRRLLDLLEQ++SC
Sbjct: 406 YMGLELAHV**PYWFQEELICSFLQASVNSGRYVLR*HDHNRDVPERRRLLDLLEQIVSC 227
Query: 480 HARHAVVCDDQVHSHI 433
H RHAVV DD+VH H+
Sbjct: 226 HPRHAVVSDDEVHVHL 179
Score = 113 bits (242), Expect(2) = 3e-078
Identities = 48/67 (71%), Positives = 57/67 (85%)
Frame = +1 / +2
Query: 244 FHVLAVDDSLPDRKLIERLLKTSSFQVTTVDSGSKALQFLGLHDQDSTVPPVHTHQLDVA 423
FHVLAVDDS+ DRKLIERLLKTSSFQVTTVDSG+KALQFLGLH +D+++ VH QLDV
Sbjct: 2 FHVLAVDDSVLDRKLIERLLKTSSFQVTTVDSGTKALQFLGLHAEDASISSVHADQLDVE 181
Query: 424 ANQDVAV 444
N ++ +
Sbjct: 182 VNVNLII 202
Score = 91.3 bits (193), Expect(3) = 5e-059
Identities = 37/56 (66%), Positives = 42/56 (75%)
Frame = -1 / -3
Query: 410 WCVWTGGTVLSWSWRPRNCSALLPESTVVTWKEEVLRSLSMSFLSGRLSSTARTWN 243
W WT S +W P+N AL+PESTVVTW E+VLRSLSMSFLS LSSTA+TWN
Sbjct: 168 WSAWTEEMEASSAWSPKNWRALVPESTVVTWNEDVLRSLSMSFLSRTLSSTAKTWN 1
Score = 89.0 bits (188), Expect(3) = 2e-049
Identities = 38/58 (65%), Positives = 46/58 (79%)
Frame = -3 / -2
Query: 417 IQLVRVDRRNRAVLVMEAQELQRLAPGVDSGHLEGRGLEEPLDELPVREAVVHRQDME 244
+QLVR+DR N VL ME QEL+ L P + SG+LE R LEEPLDELPV++AV+H QDME
Sbjct: 175 VQLVRMDRGNGGVLGMEPQELESLGPRIHSGNLERRCLEEPLDELPVKDAVIHGQDME 2
Score = 89.0 bits (188), Expect(3) = 4e-028
Identities = 44/64 (68%), Positives = 46/64 (71%)
Frame = -2 / -1
Query: 676 CICFSVCGA*ACSCLIAAQVLGRTRQLLPPGTY*SLKECSQRT*SPPGYL*ETTTP*SS* 497
CICFS+ GA*ACSCLIA V GRT LLPPG * K CS T*S PG *+TTTP*SS*
Sbjct: 422 CICFSLYGA*ACSCLIAVLVSGRTHLLLPPGIC*FWKVCSPMT*SQPGCP*KTTTP*SS* 243
Query: 496 AGHI 485
A I
Sbjct: 242 ADRI 231
Score = 46.4 bits (95), Expect(3) = 4e-028
Identities = 15/23 (65%), Positives = 20/23 (86%)
Frame = -1 / -2
Query: 152 LEEVISCHARHAVVCDDQVHSHI 84
LE+++SCH RHAVV DD+VH H+
Sbjct: 247 LEQIVSCHPRHAVVSDDEVHVHL 179
Score = 46.4 bits (95), Expect(3) = 1e-044
Identities = 17/26 (65%), Positives = 21/26 (80%)
Frame = +3 / +1
Query: 282 EAHREAPQDLFLPSDHCRLREQGAAV 359
EAHREAPQD+F+PS HC +QG+ V
Sbjct: 40 EAHREAPQDIFVPSYHCGFWDQGSPV 117
Score = 37.7 bits (76), Expect(3) = 1e-044
Identities = 16/25 (64%), Positives = 16/25 (64%)
Frame = +2 / +3
Query: 341 GARRCSSWASMTRTARFLLSTRTSW 415
G R SSW SM RT F LS RTSW
Sbjct: 99 GPRLSSSWGSMPRTPPFPLSMRTSW 173
Score = 32.7 bits (65), Expect(3) = 4e-028
Identities = 14/22 (63%), Positives = 17/22 (77%)
Frame = -1 / -2
Query: 68 IQLVRVDRRNRAVLVMEAQELQ 3
+QLVR+DR N VL ME QEL+
Sbjct: 175 VQLVRMDRGNGGVLGMEPQELE 110
Score = 26.3 bits (51), Expect(3) = 5e-059
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = -2 / -1
Query: 784 RCLHPFSCGCLCYRCC 737
RC +P+ C YRCC
Sbjct: 626 RCCYPWGYCC*WYRCC 579
Score = 24.9 bits (48), Expect(3) = 2e-049
Identities = 7/13 (53%), Positives = 8/13 (61%)
Frame = -2 / -1
Query: 757 CLCYRCC*LWGSC 719
C +RCC WG C
Sbjct: 638 CCYHRCCYPWGYC 600
>gb|AL504719.1|AL504719 AL504719 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW06C24V 5', mRNA sequence
Length = 699
Score = 149 bits (321), Expect(3) = 1e-072
Identities = 63/67 (94%), Positives = 65/67 (97%)
Frame = +1 / +2
Query: 433 DVAVNLIITDYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINRCLEEGADEFF 612
+V VNLIITDYCMPGMTGYDLLKKIKESSS RDIPVVIMSSENIPSRINRCLEEGA+EFF
Sbjct: 326 EVNVNLIITDYCMPGMTGYDLLKKIKESSSFRDIPVVIMSSENIPSRINRCLEEGANEFF 505
Query: 613 LKPVRLS 633
LKPVRLS
Sbjct: 506 LKPVRLS 526
Score = 135 bits (289), Expect(3) = 1e-061
Identities = 57/63 (90%), Positives = 62/63 (98%)
Frame = -1 / -1
Query: 632 DSRTGFRKNSSAPSSRHLLILEGMFSEDMITTGISLRDDDSLIFLSRSYPVMPGMQ*SVM 453
DSRTGFRKNS APSSRHLLILEGMFS+DMITTG+SL+DDDSLIFLSRSYPV+PGMQ*SVM
Sbjct: 525 DSRTGFRKNSFAPSSRHLLILEGMFSDDMITTGMSLKDDDSLIFLSRSYPVIPGMQ*SVM 346
Query: 452 IRF 444
+RF
Sbjct: 345 MRF 337
Score = 121 bits (258), Expect(3) = 1e-072
Identities = 52/82 (63%), Positives = 63/82 (76%)
Frame = +1 / +2
Query: 199 ALFRSVMAAVATETPFHVLAVDDSLPDRKLIERLLKTSSFQVTTVDSGSKALQFLGLHDQ 378
+L + A E PFHVLAVDDS+ DRKLIERLLKTSSFQVTTVDSG+KALQFLGLH +
Sbjct: 104 SLSSPMAVAAIAEAPFHVLAVDDSVLDRKLIERLLKTSSFQVTTVDSGTKALQFLGLHAE 283
Query: 379 DSTVPPVHTHQLDVAANQDVAV 444
D+++ VH QLDV N ++ +
Sbjct: 284 DASISSVHADQLDVEVNVNLII 349
Score = 113 bits (242), Expect(2) = 1e-047
Identities = 48/74 (64%), Positives = 59/74 (79%)
Frame = -3 / -3
Query: 654 GLELVHV**PHRF*EELVSSFLQAPIDP*RNVLRGHDHHRDISERRRLLDLLEQVISCHA 475
GL L+ **P+ F EEL+ SFLQA ++ R VLR HDH+RD+ ERRRLLDLLEQ++SCH
Sbjct: 547 GLSLLMS**PYWFQEELICSFLQASVNSGRYVLR*HDHNRDVPERRRLLDLLEQIVSCHP 368
Query: 474 RHAVVCDDQVHSHI 433
RHAVV DD+VH H+
Sbjct: 367 RHAVVSDDEVHVHL 326
Score = 105 bits (223), Expect(3) = 1e-061
Identities = 48/88 (54%), Positives = 58/88 (65%)
Frame = -3 / -3
Query: 417 IQLVRVDRRNRAVLVMEAQELQRLAPGVDSGHLEGRGLEEPLDELPVREAVVHRQDMERS 238
+QLVR+DR N VL ME QEL+ L P + SG+LE R LEEPLDELPV++AV+H QDMERS
Sbjct: 322 VQLVRMDRGNGGVLGMEPQELESLGPRIHSGNLERRCLEEPLDELPVKDAVIHGQDMERS 143
Query: 237 LCGHRRHDRSKQGSSRAAGKNWGCRGRK 154
LC R R + + GC RK
Sbjct: 142 LCDRRHRHRRR*RACVLCDGGGGC*RRK 59
Score = 97.3 bits (206), Expect(2) = 1e-047
Identities = 40/63 (63%), Positives = 45/63 (71%)
Frame = -1 / -1
Query: 410 WCVWTGGTVLSWSWRPRNCSALLPESTVVTWKEEVLRSLSMSFLSGRLSSTARTWNGVSV 231
W WT S +W P+N AL+PESTVVTW E+VLRSLSMSFLS LSSTA+TWNG S
Sbjct: 315 WSAWTEEMEASSAWSPKNWRALVPESTVVTWNEDVLRSLSMSFLSRTLSSTAKTWNGASA 136
Query: 230 ATA 222
A
Sbjct: 135 IAA 127
Score = 95.5 bits (202), Expect(3) = 6e-033
Identities = 42/71 (59%), Positives = 52/71 (73%)
Frame = +3 / +1
Query: 444 EPDHHRLLHAWHDRI*PAQEDQGVVVSQRYPGGDHVL*EHSFKDQ*VPGGRS*RVLPKTC 623
EP HH LLHAW DRI AQEDQGVVV Q +PG DHV+ EH+F++ +PGGRS VLP+T
Sbjct: 337 EPHHH*LLHAWDDRIRSAQEDQGVVVFQGHPGCDHVIGEHTFQN*QMPGGRSK*VLPETS 516
Query: 624 AAIRHEQAQAP 656
AI+ + +P
Sbjct: 517 TAIKT*ASSSP 549
Score = 46.4 bits (95), Expect(3) = 6e-033
Identities = 17/26 (65%), Positives = 21/26 (80%)
Frame = +3 / +1
Query: 282 EAHREAPQDLFLPSDHCRLREQGAAV 359
EAHREAPQD+F+PS HC +QG+ V
Sbjct: 187 EAHREAPQDIFVPSYHCGFWDQGSPV 264
Score = 42.8 bits (87), Expect(3) = 1e-072
Identities = 15/32 (46%), Positives = 20/32 (62%)
Frame = +1 / +3
Query: 634 DMNKLKPHILKSRCNQEQHQQSDSHSGERRNP 729
DM+KLKPHI+KSRC + H+ +R P
Sbjct: 528 DMSKLKPHIMKSRCKEHSHEDQRPAKQQRYQP 623
Score = 37.7 bits (76), Expect(3) = 6e-033
Identities = 16/25 (64%), Positives = 16/25 (64%)
Frame = +2 / +3
Query: 341 GARRCSSWASMTRTARFLLSTRTSW 415
G R SSW SM RT F LS RTSW
Sbjct: 246 GPRLSSSWGSMPRTPPFPLSMRTSW 320
Score = 35.9 bits (72), Expect(3) = 1e-061
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = -2 / -1
Query: 676 CICFSVCGA*ACSCL 632
CICFS+ GA*ACSCL
Sbjct: 570 CICFSLYGA*ACSCL 526
>gb|BJ461404.1|BJ461404 BJ461404 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak44m13 3', mRNA sequence
Length = 651
Score = 188 bits (406), Expect = 4e-047
Identities = 79/99 (79%), Positives = 85/99 (85%)
Frame = +1 / -2
Query: 448 LIITDYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINRCLEEGADEFFLKPVR 627
LIITDYCM GMTGYDLLKKIKESSS RDIPVVIMSSENIPSRINRCLEEGA+EFFLKPVR
Sbjct: 650 LIITDYCMXGMTGYDLLKKIKESSSFRDIPVVIMSSENIPSRINRCLEEGANEFFLKPVR 471
Query: 628 LSDMNKLKPHILKSRCNQEQHQQSDSHSGERRNPTISSS 744
LSDM+KLKPHI+KSRC + H+ D S NPT +SS
Sbjct: 470 LSDMSKLKPHIMKSRCKEHSHEDQDLLSNSDTNPTNNSS 354
Score = 149 bits (319), Expect(3) = 4e-039
Identities = 63/73 (86%), Positives = 69/73 (94%)
Frame = -1 / +1
Query: 665 FSMWGLSLFMSDSRTGFRKNSSAPSSRHLLILEGMFSEDMITTGISLRDDDSLIFLSRSY 486
F +WGLSL MSDSRTGFRKNS APSSRHLLILEGMFS+DMITTG+SL+DDDSLIFLSRSY
Sbjct: 433 FIIWGLSLLMSDSRTGFRKNSFAPSSRHLLILEGMFSDDMITTGMSLKDDDSLIFLSRSY 612
Query: 485 PVMPGMQ*SVMIR 447
PV+P MQ*SVM+R
Sbjct: 613 PVIPXMQ*SVMMR 651
Score = 132 bits (283), Expect = 4e-030
Identities = 58/100 (58%), Positives = 71/100 (71%)
Frame = +3 / -1
Query: 447 PDHHRLLHAWHDRI*PAQEDQGVVVSQRYPGGDHVL*EHSFKDQ*VPGGRS*RVLPKTCA 626
P HH LLHAW DRI AQEDQGVVV Q +PG DHV+ EH+F++ +PGGRS VLP+T
Sbjct: 651 PHHH*LLHAWDDRIRSAQEDQGVVVFQGHPGCDHVIGEHTFQN*QMPGGRSK*VLPETST 472
Query: 627 AIRHEQAQAPHTEKQMQPGTAPAK*QSQWRTQEPHNQQQR 746
AIRHEQAQAP+ EKQMQ ++ + +PH QQQ+
Sbjct: 471 AIRHEQAQAPYNEKQMQGALPRGPRPAKQQRYQPHKQQQQ 352
Score = 114 bits (244), Expect(2) = 7e-026
Identities = 48/71 (67%), Positives = 58/71 (81%)
Frame = -3 / +3
Query: 660 YVGLELVHV**PHRF*EELVSSFLQAPIDP*RNVLRGHDHHRDISERRRLLDLLEQVISC 481
Y+GLEL HV**P+ F EEL+ SFLQA ++ R VLR HDH+RD+ ERRRLLDLLEQ++SC
Sbjct: 438 YMGLELAHV**PYWFQEELICSFLQASVNSGRYVLR*HDHNRDVPERRRLLDLLEQIVSC 617
Query: 480 HARHAVVCDDQ 448
H HAVV DD+
Sbjct: 618 HPXHAVVSDDE 650
Score = 89.0 bits (188), Expect(3) = 2e-022
Identities = 44/64 (68%), Positives = 46/64 (71%)
Frame = -2 / +2
Query: 676 CICFSVCGA*ACSCLIAAQVLGRTRQLLPPGTY*SLKECSQRT*SPPGYL*ETTTP*SS* 497
CICFS+ GA*ACSCLIA V GRT LLPPG * K CS T*S PG *+TTTP*SS*
Sbjct: 422 CICFSLYGA*ACSCLIAVLVSGRTHLLLPPGIC*FWKVCSPMT*SQPGCP*KTTTP*SS* 601
Query: 496 AGHI 485
A I
Sbjct: 602 ADRI 613
Score = 34.5 bits (69), Expect(3) = 2e-022
Identities = 11/18 (61%), Positives = 15/18 (83%)
Frame = -1 / +3
Query: 152 LEEVISCHARHAVVCDDQ 99
LE+++SCH HAVV DD+
Sbjct: 597 LEQIVSCHPXHAVVSDDE 650
Score = 33.1 bits (66), Expect(3) = 4e-039
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = -2 / +2
Query: 784 RCLHPFSCGCLCYRCC 737
R L PFSCGC CY C
Sbjct: 176 RHLPPFSCGCCCYHRC 223
Score = 26.3 bits (51), Expect(2) = 7e-026
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = -2 / +2
Query: 784 RCLHPFSCGCLCYRCC 737
RC +P+ C YRCC
Sbjct: 218 RCCYPWGYCC*WYRCC 265
Score = 24.9 bits (48), Expect(3) = 2e-022
Identities = 7/13 (53%), Positives = 8/13 (61%)
Frame = -2 / +2
Query: 757 CLCYRCC*LWGSC 719
C +RCC WG C
Sbjct: 206 CCYHRCCYPWGYC 244
Score = 23.1 bits (44), Expect(3) = 4e-039
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = -2 / +2
Query: 865 KRKGFSCTSQFSVNCALICL 806
+RK + + S NC L+CL
Sbjct: 101 ERKDWLSNNLSSANCGLVCL 160
>gb|BI778941.2|BI778941 EBro01_SQ002_C23_R root, 3 week, hydroponic grown, no treatment, cv
Optic, EBro01 Hordeum vulgare subsp. vulgare cDNA clone
EBro01_SQ002_C23 5', mRNA sequence
Length = 568
Score = 128 bits (274), Expect(2) = 2e-043
Identities = 53/79 (67%), Positives = 66/79 (83%)
Frame = +1 / +2
Query: 412 LDVAANQDVAVNLIITDYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINRCLE 591
L+V + +V LIITDYCMP MTGYDLLK++KES+ LR IPVVIMSSEN P+RI RCL+
Sbjct: 248 LEVLSLGHQSVQLIITDYCMPEMTGYDLLKRVKESAELRGIPVVIMSSENSPARIRRCLD 427
Query: 592 EGADEFFLKPVRLSDMNKL 648
EGA+EF +KPVR SD+++L
Sbjct: 428 EGAEEFLIKPVRPSDVSRL 484
Score = 82.6 bits (174), Expect(2) = 4e-023
Identities = 39/62 (62%), Positives = 44/62 (70%)
Frame = -1 / -2
Query: 635 SDSRTGFRKNSSAPSSRHLLILEGMFSEDMITTGISLRDDDSLIFLSRSYPVMPGMQ*SV 456
S+ RTG +NSSAPSSRH IL G FS+DMITTG+ DS SRS PV+ GMQ*SV
Sbjct: 471 SEGRTGLMRNSSAPSSRHRRILAGEFSDDMITTGMPRSSADSFTRFSRS*PVISGMQ*SV 292
Query: 455 MI 450
MI
Sbjct: 291 MI 286
Score = 68.4 bits (143), Expect(2) = 2e-043
Identities = 30/52 (57%), Positives = 38/52 (73%)
Frame = +1 / +2
Query: 223 AVATETPFHVLAVDDSLPDRKLIERLLKTSSFQVTTVDSGSKALQFLGLHDQ 378
+ + P HVLAVDDSL DR +I RLL++S ++VTTVDSG KAL+ L L Q
Sbjct: 119 STTVQEPPHVLAVDDSLVDRVVISRLLRSSKYRVTTVDSGKKALEVLSLGHQ 274
Score = 46.0 bits (94), Expect(2) = 4e-023
Identities = 25/46 (54%), Positives = 27/46 (58%)
Frame = -1 / -2
Query: 377 WSWRPRNCSALLPESTVVTWKEEVLRSLSMSFLSGRLSSTARTWNG 240
W R SA P STVVT E+ RSL M+ S RLSSTART G
Sbjct: 273 WCPRLSTSSAFFPLSTVVTLYLELRRSLEMTTRSTRLSSTARTCGG 136
>gb|CA012046.1|CA012046 HT04E15r HT Hordeum vulgare subsp. vulgare cDNA clone HT04E15
5-PRIME, mRNA sequence
Length = 533
Score = 109 bits (233), Expect(2) = 1e-036
Identities = 43/54 (79%), Positives = 51/54 (94%)
Frame = +1 / +2
Query: 442 VNLIITDYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINRCLEEGAD 603
VN+IITDY MPGMTGY+LLK++KESS LR+IPVVIMSSEN+P+RI RCLEEGA+
Sbjct: 371 VNMIITDYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITRCLEEGAE 532
Score = 64.8 bits (135), Expect(2) = 1e-036
Identities = 26/52 (50%), Positives = 39/52 (75%)
Frame = +1 / +2
Query: 214 VMAAVATETPFHVLAVDDSLPDRKLIERLLKTSSFQVTTVDSGSKALQFLGL 369
V+ V + HVLAVDDS+ DR +I ++L++S ++VTTVDS ++AL+ LGL
Sbjct: 200 VVVQVQVQAELHVLAVDDSVVDRAVIAKILRSSKYRVTTVDSATRALELLGL 355
>gb|BQ766925.1|BQ766925 EBro08_SQ007_B17_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ007_B17 5', mRNA sequence
Length = 554
Score = 97.7 bits (207), Expect(2) = 4e-033
Identities = 38/48 (79%), Positives = 45/48 (93%)
Frame = +1 / +3
Query: 442 VNLIITDYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINRC 585
VN+IITDY MPGMTGY+LLK++KESS LR+IPVVIMSSEN+P+RI RC
Sbjct: 411 VNMIITDYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITRC 554
Score = 64.8 bits (135), Expect(2) = 4e-033
Identities = 26/52 (50%), Positives = 39/52 (75%)
Frame = +1 / +3
Query: 214 VMAAVATETPFHVLAVDDSLPDRKLIERLLKTSSFQVTTVDSGSKALQFLGL 369
V+ V + HVLAVDDS+ DR +I ++L++S ++VTTVDS ++AL+ LGL
Sbjct: 240 VVVQVQVQAELHVLAVDDSVVDRAVIAKILRSSKYRVTTVDSATRALELLGL 395
>gb|CA008912.1|CA008912 HU12I03r HU Hordeum vulgare subsp. vulgare cDNA clone HU12I03
5-PRIME, mRNA sequence
Length = 488
Score = 131 bits (281), Expect(2) = 7e-033
Identities = 52/69 (75%), Positives = 64/69 (92%)
Frame = +1 / +3
Query: 442 VNLIITDYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINRCLEEGADEFFLKP 621
VN+IITDY MPGMTGY+LLK++KESS LR+IPVVIMSSEN+P+RI RCLEEGA++F LKP
Sbjct: 84 VNMIITDYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITRCLEEGAEDFLLKP 263
Query: 622 VRLSDMNKL 648
VR SD+++L
Sbjct: 264 VRPSDVSRL 290
Score = 29.9 bits (59), Expect(2) = 7e-033
Identities = 11/18 (61%), Positives = 16/18 (88%)
Frame = +1 / +3
Query: 316 FQVTTVDSGSKALQFLGL 369
++VTTVDS ++AL+ LGL
Sbjct: 15 YRVTTVDSATRALELLGL 68
>gb|BQ762675.1|BQ762675 EBro02_SQ004_O15 _R root, 3 week, hydroponic grown, low nitrogen,
cv Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA
clone EBro02_SQ004_O15 5', mRNA sequence
Length = 571
Score = 93.6 bits (198), Expect(2) = 6e-032
Identities = 37/47 (78%), Positives = 44/47 (93%)
Frame = +1 / +1
Query: 442 VNLIITDYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINR 582
VN+IITDY MPGMTGY+LLK++KESS LR+IPVVIMSSEN+P+RI R
Sbjct: 430 VNMIITDYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITR 570
Score = 64.8 bits (135), Expect(2) = 6e-032
Identities = 26/52 (50%), Positives = 39/52 (75%)
Frame = +1 / +1
Query: 214 VMAAVATETPFHVLAVDDSLPDRKLIERLLKTSSFQVTTVDSGSKALQFLGL 369
V+ V + HVLAVDDS+ DR +I ++L++S ++VTTVDS ++AL+ LGL
Sbjct: 259 VVVQVQVQAELHVLAVDDSVVDRAVIAKILRSSKYRVTTVDSATRALELLGL 414
>gb|AW982401.2|AW982401 HVSMEg0003C19f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0003C19f, mRNA sequence
Length = 873
Score = 135 bits (290), Expect = 4e-031
Identities = 53/76 (69%), Positives = 68/76 (89%)
Frame = +1 / +3
Query: 466 CMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINRCLEEGADEFFLKPVRLSDMNK 645
CMPGMTGYDLL+++K SSSL+DIPVVIMSSEN+P+RI+RCLE+GA+EFFLKPV+L+DM K
Sbjct: 3 CMPGMTGYDLLRRVKGSSSLKDIPVVIMSSENVPARISRCLEDGAEEFFLKPVKLADMKK 182
Query: 646 LKPHILKSRCNQEQHQ 693
LK H+++ + Q Q Q
Sbjct: 183 LKSHLVRRKQPQLQPQ 230
Score = 110 bits (235), Expect(2) = 1e-025
Identities = 48/62 (77%), Positives = 51/62 (82%)
Frame = -1 / -3
Query: 650 LSLFMSDSRTGFRKNSSAPSSRHLLILEGMFSEDMITTGISLRDDDSLIFLSRSYPVMPG 471
LS FMS S TG RKNSSAPSS+HLLIL G FSEDMITTG+S +DD L LSRSYPVMPG
Sbjct: 187 LSFFMSASFTGLRKNSSAPSSKHLLILAGTFSEDMITTGMSFSEDDPLTLLSRSYPVMPG 8
Query: 470 MQ 465
MQ
Sbjct: 7 MQ 2
Score = 26.7 bits (52), Expect(2) = 1e-025
Identities = 11/25 (44%), Positives = 11/25 (44%)
Frame = -2 / -3
Query: 742 CC*LWGSCVRHCDCHFAGAVPGCIC 668
CC SCV C C G GC C
Sbjct: 301 CCCRGFSCVCGCCCSVCGFCCGCGC 227
>gb|BQ465184.1|BQ465184 HU02O05r HU Hordeum vulgare subsp. vulgare cDNA clone HU02O05
5-PRIME, mRNA sequence
Length = 627
Score = 131 bits (281), Expect = 8e-030
Identities = 52/69 (75%), Positives = 64/69 (92%)
Frame = +1 / +1
Query: 442 VNLIITDYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINRCLEEGADEFFLKP 621
VN+IITDY MPGMTGY+LLK++KESS LR+IPVVIMSSEN+P+RI RCLEEGA++F LKP
Sbjct: 16 VNMIITDYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITRCLEEGAEDFLLKP 195
Query: 622 VRLSDMNKL 648
VR SD+++L
Sbjct: 196 VRPSDVSRL 222
>gb|BI947127.1|BI947127 HVSMEl0003N07f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0003N07f, mRNA sequence
Length = 1057
Score = 116 bits (249), Expect(2) = 2e-029
Identities = 51/83 (61%), Positives = 62/83 (74%)
Frame = +1 / +3
Query: 199 ALFRSVMAAVATETPFHVLAVDDSLPDRKLIERLLKTSSFQVTTVDSGSKALQFLGLHDQ 378
+L + A E PFHVLAVDDS+ DRKLIERLLKTSSFQVTTVDSG+KALQFLGLH +
Sbjct: 96 SLSSPMAVAAIAEAPFHVLAVDDSVLDRKLIERLLKTSSFQVTTVDSGTKALQFLGLHAE 275
Query: 379 DSTVPPVHTHQLDVAANQDVAVN 447
D+ + H QLD+ N ++ +N
Sbjct: 276 DAFISSGHADQLDLQVNLNLILN 344
Score = 102 bits (217), Expect(2) = 5e-024
Identities = 43/81 (53%), Positives = 55/81 (67%)
Frame = -3 / -3
Query: 447 VHSHILIGSHIQLVRVDRRNRAVLVMEAQELQRLAPGVDSGHLEGRGLEEPLDELPVREA 268
+ + + +QLVR+ R N VL ME QEL+ L P + SG+LE R LEEPLDELPV++A
Sbjct: 344 IEDKVKVNLEVQLVRMARGNEGVLGMEPQELESLGPRIHSGNLERRCLEEPLDELPVKDA 165
Query: 267 VVHRQDMERSLCGHRRHDRSK 205
V+H QDMERSLC R R +
Sbjct: 164 VIHGQDMERSLCDRRHRHRRR 102
Score = 33.1 bits (66), Expect(2) = 2e-029
Identities = 15/33 (45%), Positives = 22/33 (66%)
Frame = +1 / +3
Query: 436 VAVNLIITDYCMPGMTGYDLLKKIKESSSLRDI 534
V +NLI+ + +PG+TG LKKI+E S + I
Sbjct: 321 VNLNLILNE*RLPGITGNQSLKKIQEPVSFQGI 419
Score = 29.5 bits (58), Expect(2) = 5e-024
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = -1 / -1
Query: 524 RDDDSLIFLSRSYPVMPGMQ*SVMIR 447
+D S IFLS +PV+PG + S+ IR
Sbjct: 409 KDTGSWIFLSDWFPVIPGSRYSLRIR 332
>gb|BF064456.3|BF064456 HV_CEb0015C20f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0015C20f, mRNA sequence
Length = 622
Score = 124 bits (265), Expect = 1e-027
Identities = 49/65 (75%), Positives = 60/65 (92%)
Frame = +1 / +3
Query: 454 ITDYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINRCLEEGADEFFLKPVRLS 633
ITDY MPGMTGY+LLK++KESS LR+IPVVIMSSEN+P+RI RCLEEGA++F LKPVR S
Sbjct: 3 ITDYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITRCLEEGAEDFLLKPVRPS 182
Query: 634 DMNKL 648
D+++L
Sbjct: 183 DVSRL 197
>gb|BQ663493.1|BQ663493 HU02O05u HU Hordeum vulgare subsp. vulgare cDNA clone HU02O05
3-PRIME, mRNA sequence
Length = 593
Score = 120 bits (256), Expect = 2e-026
Identities = 47/63 (74%), Positives = 58/63 (92%)
Frame = +1 / -1
Query: 460 DYCMPGMTGYDLLKKIKESSSLRDIPVVIMSSENIPSRINRCLEEGADEFFLKPVRLSDM 639
DY MPGMTGY+LLK++KESS LR+IPVVIMSSEN+P+RI RCLEEGA++F LKPVR SD+
Sbjct: 593 DYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITRCLEEGAEDFLLKPVRPSDV 414
Query: 640 NKL 648
++L
Sbjct: 413 SRL 405
>gb|AL501583.1|AL501583 AL501583 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW04L02u 3', mRNA sequence
Length = 619
Score = 97.7 bits (207), Expect(2) = 1e-021
Identities = 39/57 (68%), Positives = 45/57 (78%)
Frame = +1 / -2
Query: 574 INRCLEEGADEFFLKPVRLSDMNKLKPHILKSRCNQEQHQQSDSHSGERRNPTISSS 744
INRCLEEGA+EFFLKPVRLSDM+KLKPHI+KSRC + H+ D S NPT +SS
Sbjct: 552 INRCLEEGANEFFLKPVRLSDMSKLKPHIMKSRCKEHSHEDQDLLSNSDTNPTNNSS 382
Score = 29.0 bits (57), Expect(2) = 1e-021
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = +2 / -2
Query: 914 FAKRNGICKLILLESNRSK 970
FAK+NGI + ILLES + K
Sbjct: 63 FAKKNGIRERILLESKKKK 7
>gb|BG344635.2|BG344635 HVSMEg0009O02f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0009O02f, mRNA sequence
Length = 835
Score = 62.9 bits (131), Expect(2) = 2e-021
Identities = 25/51 (49%), Positives = 38/51 (74%)
Frame = +1 / +1
Query: 214 VMAAVATETPFHVLAVDDSLPDRKLIERLLKTSSFQVTTVDSGSKALQFLG 366
V+ V + HVLAVDDS+ DR +I ++L++S ++VTTVDS ++AL+ LG
Sbjct: 235 VVVQVQVQAELHVLAVDDSVVDRAVIAKILRSSKYRVTTVDSATRALELLG 387
Score = 60.2 bits (125), Expect(2) = 2e-021
Identities = 24/35 (68%), Positives = 30/35 (85%)
Frame = +1 / +1
Query: 442 VNLIITDYCMPGMTGYDLLKKIKESSSLRDIPVVI 546
VN+IITDY MPGMTGY+LLK++KE + LR+IP I
Sbjct: 406 VNMIITDY*MPGMTGYELLKRLKEWTELREIPWTI 510
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 645,337,547
Number of Sequences: 312970
Number of extensions: 10753721
Number of successful extensions: 108153
Number of sequences better than 1.0e-020: 21
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 16465562986
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)