TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441276.2.1
(1796 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG299933.1|BG299933 HVSMEa0022J07f Hordeum vulgare seedl... 108 7e-058
gb|BF622955.2|BF622955 HVSMEa0012E13f Hordeum vulgare seedl... 101 2e-041
gb|AV934271.1|AV934271 AV934271 K. Sato unpublished cDNA li... 87 4e-030
gb|BJ472357.1|BJ472357 BJ472357 K. Sato unpublished cDNA li... 100 1e-029
gb|BU987774.1|BU987774 HF15L16r HF Hordeum vulgare subsp. v... 94 7e-026
gb|CA032804.1|CA032804 HX14E02r HX Hordeum vulgare subsp. v... 94 3e-025
gb|CA031681.1|CA031681 HX10N04r HX Hordeum vulgare subsp. v... 73 8e-023
gb|BG343130.1|BG343130 HVSMEg0002J04f Hordeum vulgare pre-a... 54 8e-023
>gb|BG299933.1|BG299933 HVSMEa0022J07f Hordeum vulgare seedling shoot EST library HVcDNA0001
(Cold stress) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEa0022J07f, mRNA sequence
Length = 787
Score = 108 bits (230), Expect(4) = 7e-058
Identities = 44/56 (78%), Positives = 47/56 (83%)
Frame = -2 / +3
Query: 1246 FAESAQFHKVLQGQELLGYRTHDNAAVATSQPCEATNMQYIDERSCSNDASNIIPG 1079
FAESAQ KVLQGQELLGYRTHD AVATSQPCEA N+QYIDER CSN+ SN + G
Sbjct: 3 FAESAQLRKVLQGQELLGYRTHDGTAVATSQPCEARNLQYIDERGCSNNGSNNVLG 170
Score = 97.7 bits (207), Expect(4) = 7e-058
Identities = 38/57 (66%), Positives = 42/57 (73%)
Frame = -2 / +3
Query: 1030 YRCSGFGESPRFQKVLQGQEVFHPYRGTLVDASLSNSGFHQQDGSHVPTQASKWHAQ 860
Y CSGFGES RFQKVLQGQEVF PYRG+LVDA + + G HQQDG + KW AQ
Sbjct: 213 YHCSGFGESQRFQKVLQGQEVFRPYRGSLVDARMRSGGSHQQDGPYASALLDKWRAQ 383
Score = 94.5 bits (200), Expect(4) = 5e-043
Identities = 40/62 (64%), Positives = 44/62 (70%)
Frame = +1 / -2
Query: 1063 HQFLEPPG*CYSHRWSNFVHQCTACSLLRMAEKLQQQHCHEFYNPVILDLARPCGIGQIP 1242
H P G CYSH SN VH+CTA LL MA K QQQ+ HEFYNP+ILDLARPCG+ Q
Sbjct: 186 HGLGPPLGHCYSHY*SNLVHRCTASFLLHMAVK*QQQYHHEFYNPIILDLARPCGVVQTL 7
Query: 1243 QN 1248
QN
Sbjct: 6 QN 1
Score = 89.5 bits (189), Expect(3) = 1e-038
Identities = 41/84 (48%), Positives = 56/84 (66%)
Frame = -3 / +1
Query: 1041 LGFPTVAQALGSLQDSKRSCKVKKYFIPTEELWSMQA*VIVASISKMVLMCLLRPASGTH 862
LGFPT+A ALGSL+DSKRSCKVKK+ + TEE+W M+A* + A I++MVLM L +G
Sbjct: 202 LGFPTIALALGSLRDSKRSCKVKKFSVRTEEVWLMRA*EVAALINRMVLMHLPCWINGAR 381
Query: 861 SYMDVLFVANKHQLFHLNPHPHHL 790
+ ++H+ L+ HHL
Sbjct: 382 NNSMHSVTGHQHKFCRLSHRYHHL 453
Score = 85.8 bits (181), Expect(4) = 4e-046
Identities = 41/56 (73%), Positives = 45/56 (80%)
Frame = +3 / -1
Query: 1080 PGIMLLASLEQLRSSMYCMFVASHG*EVATAALS*VL*PSNS*PCKTLWNWADSAK 1247
P +LL LEQ RSSMYC F+ASHG EVATA S*VL*P+NS*PCKTL + ADSAK
Sbjct: 169 PRTLLLPLLEQPRSSMYCKFLASHGCEVATAVPS*VL*PNNS*PCKTLRSCADSAK 2
Score = 82.2 bits (173), Expect(2) = 1e-030
Identities = 39/56 (69%), Positives = 42/56 (75%)
Frame = +2 / -3
Query: 1079 PRDNVTRIVGATSFINVLHVRCFAWLRSCNSSIVMSSITQ*FLTLQDLVELGRFRK 1246
P D VT I+ ATSFI+VL V CF WL S NSS +MSSITQ*FLTLQDL EL R K
Sbjct: 170 P*DIVTPIIRATSFIDVLQVSCFTWL*SSNSSTIMSSITQ*FLTLQDLAELCRLCK 3
Score = 79.4 bits (167), Expect(4) = 4e-046
Identities = 37/57 (64%), Positives = 39/57 (68%)
Frame = +3 / -1
Query: 855 CSCACHLLA*VGT*EPSC*WKPLLLKLASTRVPL*G*NTS*PCKTFWNLGDSPKPEQ 1025
C CA HL * PSC* +P LL ASTR+P G TS*PCKTFWNL DSPKPEQ
Sbjct: 388 CCCARHLSNRADA*GPSC**EPPLLMRASTRLPRYGRKTS*PCKTFWNL*DSPKPEQ 218
Score = 78.5 bits (165), Expect(3) = 1e-038
Identities = 36/66 (54%), Positives = 41/66 (62%)
Frame = -3 / +1
Query: 1248 ILRNLPNSTRSCKVKNYWVIEXXXXXXXXXLSHAKQRTCSTLMNEVAPTMRVTLSRGFQE 1069
IL++L NS RSCKVKNYWVIE SH KQ TCST MNEVA M VT+S G +
Sbjct: 1 ILQSLHNSARSCKVKNYWVIELMMVLLLLLHSHVKQETCSTSMNEVALIMGVTMS*GGSQ 180
Query: 1068 LVSEHH 1051
+ H
Sbjct: 181 AMV*EH 198
Score = 77.6 bits (163), Expect(4) = 5e-043
Identities = 37/76 (48%), Positives = 47/76 (61%)
Frame = +1 / -2
Query: 811 EMEQLVLVGHEKHIHVAVRATCWPE*AHENHLADGSHYYSSLHRPEFLCRDEILLDLARP 990
E +LVLV + V RA H++H D S ++S H+P+FL D LLDLARP
Sbjct: 432 ETAKLVLVARNRMHAVVARAIYPTGQMHKDHPVDESRHFSCAHQPDFLGTDGKLLDLARP 253
Query: 991 FGILETPQSLSNGRKT 1038
FGI ETPQS SNGR++
Sbjct: 252 FGISETPQSQSNGRES 205
Score = 72.1 bits (151), Expect(2) = 1e-030
Identities = 40/84 (47%), Positives = 48/84 (57%)
Frame = +2 / -3
Query: 791 RWWG*GLRWNSWCLLATKSTSM*LCVPLAGLSRHMRTILLMEATITQACIDQSSSVGMKY 970
RW LR + C + L P R +RTILLM A + A I+Q+SSV +
Sbjct: 452 RW**RWLRRQNLCWWPVTECMLLLRAPFIQQGRCIRTILLMRAATSHARINQTSSVRTEN 273
Query: 971 FLTLQDLLESWRLPKA*ATVGKPR 1042
FLTLQDLLES RLPKA A VG P+
Sbjct: 272 FLTLQDLLESLRLPKARAMVGNPK 201
Score = 69.8 bits (146), Expect(3) = 1e-026
Identities = 38/79 (48%), Positives = 45/79 (56%)
Frame = -1 / +2
Query: 1031 LPLLRLWGVSKIPKGLARSRSISSLQRNSGRCKLE**WLPSARWFSCAYSGQQVARTATW 852
LPLL LWGVS+IPKGLARSRS S+ R SG C E L S W C +AR T
Sbjct: 212 LPLLWLWGVSEIPKGLARSRSFPSVPRKSG*CAHEKWRLSSTGWSLCICPVG*MARATTA 391
Query: 851 MCFSWPTSTSCSISILIPT 795
TSTS ++S ++ T
Sbjct: 392 CIRLRATSTSFAVSAIVIT 448
Score = 59.7 bits (124), Expect(3) = 1e-026
Identities = 34/57 (59%), Positives = 38/57 (66%)
Frame = -1 / +2
Query: 1247 FCGICPIPQGLARSRITGL*NS*QXXXCNFSAMRSNEHAVH**TKLLQRCE*HYPGG 1077
FC +C PQGLARSRI GL*NS* C F+AM S + AVH *T+LL E* P G
Sbjct: 2 FCRVCTTPQGLARSRIIGL*NS*WYCCCYFTAM*SKKLAVHR*TRLL**WE*QCPRG 172
Score = 49.6 bits (102), Expect(4) = 7e-058
Identities = 18/25 (72%), Positives = 21/25 (84%)
Frame = -2 / +3
Query: 787 LMFQRGDPKMSPFEFGHFHLNKNED 713
LMFQ DPK+S FEFGH H++KNED
Sbjct: 459 LMFQNADPKVSRFEFGHGHMDKNED 533
Score = 43.2 bits (88), Expect(4) = 4e-046
Identities = 17/29 (58%), Positives = 21/29 (72%)
Frame = +2 / -3
Query: 713 ILVLIQVEMPKFKWGHLWITSLKHQDRWW 799
IL+LI V MP+ K LWI+ LKHQ+R W
Sbjct: 533 ILILIHVPMPELKSRDLWISVLKHQNRRW 447
Score = 35.4 bits (71), Expect(4) = 5e-043
Identities = 16/25 (64%), Positives = 17/25 (68%)
Frame = +3 / -1
Query: 714 SSFLFKWKCPNSNGDIFGSPR*NIR 788
SSFL CPNSN + FGS *NIR
Sbjct: 532 SSFLSMCPCPNSNRETFGSAF*NIR 458
Score = 34.1 bits (68), Expect(4) = 4e-046
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = +3 / -1
Query: 1041 GLPSGVLTPILGTPGIMLLASL 1106
G+PSGVLTP LGTP LL L
Sbjct: 211 GIPSGVLTPWLGTPPRTLLLPL 146
Score = 31.3 bits (62), Expect(3) = 1e-038
Identities = 18/34 (52%), Positives = 23/34 (67%)
Frame = -1 / +2
Query: 812 SILIPTICPDVSTR*SKDVPI*IWAFPLE*ERG* 711
+I+I T C DVS R*SK + I*+ A+ *E G*
Sbjct: 434 AIVITTFCSDVSER*SKGLSI*VRAWAHG*E*G* 535
Score = 29.0 bits (57), Expect(3) = 1e-026
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = -3 / +1
Query: 798 HHLS*CFNEVIQRCPHLNLGIST*IRTRI 712
H L *CF +IQR L+ G+ T IR R+
Sbjct: 448 HLLF*CFRTLIQRSLDLSSGMGTWIRMRM 534
Score = 26.7 bits (52), Expect(4) = 7e-058
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = -1 / +2
Query: 1082 GGSKNWCQNTT 1050
GG K WC+NTT
Sbjct: 170 GGPKPWCENTT 202
Score = 24.4 bits (47), Expect(4) = 5e-043
Identities = 6/11 (54%), Positives = 9/11 (81%)
Frame = +2 / -3
Query: 1049 EWCSDTNSWNP 1081
+WCS T +W+P
Sbjct: 203 KWCSHTMAWDP 171
>gb|BF622955.2|BF622955 HVSMEa0012E13f Hordeum vulgare seedling shoot EST library HVcDNA0001
(Cold stress) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEa0012E13f, mRNA sequence
Length = 850
Score = 101 bits (215), Expect(6) = 2e-041
Identities = 39/57 (68%), Positives = 43/57 (75%)
Frame = -2 / +2
Query: 1030 YRCSGFGESPRFQKVLQGQEVFHPYRGTLVDASLSNSGFHQQDGSHVPTQASKWHAQ 860
Y CSGFGES RFQKVLQGQEVF PYRG+LVDA + + GFHQQDG + KW AQ
Sbjct: 41 YHCSGFGESQRFQKVLQGQEVFRPYRGSLVDARMRSGGFHQQDGPYASALLDKWRAQ 211
Score = 89.5 bits (189), Expect(5) = 3e-026
Identities = 41/84 (48%), Positives = 56/84 (66%)
Frame = -3 / +3
Query: 1041 LGFPTVAQALGSLQDSKRSCKVKKYFIPTEELWSMQA*VIVASISKMVLMCLLRPASGTH 862
LGFPT+A ALGSL+DSKRSCKVKK+ + TEE+W M+A* + A I++MVLM L +G
Sbjct: 30 LGFPTIALALGSLRDSKRSCKVKKFSVRTEEVWLMRA*EVAAFINRMVLMHLPCWINGAR 209
Query: 861 SYMDVLFVANKHQLFHLNPHPHHL 790
+ ++H+ L+ HHL
Sbjct: 210 NNSMHSVTGHQHKFCRLSHRYHHL 281
Score = 81.3 bits (171), Expect(6) = 2e-038
Identities = 38/57 (66%), Positives = 39/57 (68%)
Frame = +3 / -2
Query: 855 CSCACHLLA*VGT*EPSC*WKPLLLKLASTRVPL*G*NTS*PCKTFWNLGDSPKPEQ 1025
C CA HL * PSC* KP LL ASTR+P G TS*PCKTFWNL DSPKPEQ
Sbjct: 216 CCCARHLSNRADA*GPSC**KPPLLMRASTRLPRYGRKTS*PCKTFWNL*DSPKPEQ 46
Score = 78.0 bits (164), Expect(3) = 4e-022
Identities = 37/76 (48%), Positives = 47/76 (61%)
Frame = +1 / -3
Query: 811 EMEQLVLVGHEKHIHVAVRATCWPE*AHENHLADGSHYYSSLHRPEFLCRDEILLDLARP 990
E +LVLV + V RA H++H D S ++S H+P+FL D LLDLARP
Sbjct: 260 ETAELVLVARNRMHAVVARAIYPTGQMHKDHPVDESRHFSCAHQPDFLGTDGKLLDLARP 81
Query: 991 FGILETPQSLSNGRKT 1038
FGI ETPQS SNGR++
Sbjct: 80 FGISETPQSQSNGRES 33
Score = 50.6 bits (104), Expect(6) = 2e-041
Identities = 18/25 (72%), Positives = 22/25 (88%)
Frame = -2 / +2
Query: 787 LMFQRGDPKMSPFEFGHFHLNKNED 713
LMFQ+ DPK+S FEFGH H++KNED
Sbjct: 287 LMFQKADPKVSRFEFGHGHMDKNED 361
Score = 43.2 bits (88), Expect(6) = 2e-038
Identities = 17/29 (58%), Positives = 21/29 (72%)
Frame = +2 / -1
Query: 713 ILVLIQVEMPKFKWGHLWITSLKHQDRWW 799
IL+LI V MP+ K LWI+ LKHQ+R W
Sbjct: 361 ILILIHVPMPELKSRDLWISLLKHQNRRW 275
Score = 41.8 bits (85), Expect(6) = 2e-038
Identities = 16/23 (69%), Positives = 18/23 (78%)
Frame = +1 / -1
Query: 361 RQSP*QQQASAHERWHSCCPAIS 429
RQSP QQQA AHE+WH+C IS
Sbjct: 670 RQSPCQQQALAHEQWHNCGEGIS 602
Score = 38.2 bits (77), Expect(6) = 2e-041
Identities = 16/23 (69%), Positives = 17/23 (73%)
Frame = -2 / +1
Query: 427 KSLGNSCATVHEQRPVVARVIDV 359
KS +SCATVHE PVV R IDV
Sbjct: 604 KSPRHSCATVHEPEPVVGRAIDV 672
Score = 36.3 bits (73), Expect(5) = 3e-026
Identities = 16/23 (69%), Positives = 16/23 (69%)
Frame = -1 / +3
Query: 428 EIAGQQLCHRS*AEACCC*GD*R 360
EI QLCH S*A ACC GD*R
Sbjct: 603 EIPSPQLCHCS*ARACCWQGD*R 671
Score = 36.3 bits (73), Expect(3) = 4e-022
Identities = 15/26 (57%), Positives = 19/26 (73%)
Frame = +2 / -2
Query: 359 NVNHPSNNRPLLMNGGTAVAQRFRHL 436
+VN P+NNR LMN GT VA+ F+ L
Sbjct: 672 DVNRPANNRLWLMNSGTTVARGFQGL 595
Score = 35.4 bits (71), Expect(3) = 4e-022
Identities = 16/25 (64%), Positives = 17/25 (68%)
Frame = +3 / -2
Query: 714 SSFLFKWKCPNSNGDIFGSPR*NIR 788
SSFL CPNSN + FGS *NIR
Sbjct: 360 SSFLSMCPCPNSNRETFGSAF*NIR 286
Score = 32.2 bits (64), Expect(6) = 2e-038
Identities = 19/40 (47%), Positives = 20/40 (50%)
Frame = +3 / -2
Query: 600 FSTVTSLFPVPPET**AWSIVVCSVPPMPPAWTNIARLSS 719
FST T L PE * S CS P P A TN+A SS
Sbjct: 477 FSTATRLSITSPEPR*GRSDDACSALPTPSAETNLAYQSS 358
Score = 31.3 bits (62), Expect(6) = 2e-041
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = -2 / +2
Query: 550 NSCKIFGISLTEK 512
NSCKIFGISL EK
Sbjct: 497 NSCKIFGISLAEK 535
Score = 29.5 bits (58), Expect(5) = 3e-026
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = -3 / +3
Query: 798 HHLS*CFNEVIQRCPHLNLGIST*IRTRI 712
H L *CF +IQR L+ G+ T IR R+
Sbjct: 276 HLLF*CFRRLIQRSLDLSSGMGTWIRMRM 362
Score = 29.0 bits (57), Expect(6) = 2e-038
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = +3 / -2
Query: 1041 GLPSGVLTPILGT 1079
G+PSGVLTP LGT
Sbjct: 39 GIPSGVLTPWLGT 1
Score = 25.4 bits (49), Expect(6) = 2e-041
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -2 / +2
Query: 1078 VPRIGVRTPLGSP 1040
VP GVRTPLG P
Sbjct: 2 VPSHGVRTPLGIP 40
Score = 24.4 bits (47), Expect(6) = 2e-041
Identities = 12/25 (48%), Positives = 12/25 (48%)
Frame = -2 / +1
Query: 514 KVPAMKEKGCGDINTNYPSPFLSLK 440
KV A E C D NY SP LK
Sbjct: 532 KVRARDEMVCDDGGANYSSPTQPLK 606
Score = 24.0 bits (46), Expect(5) = 3e-026
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = -1 / +1
Query: 1079 GSKNWCQNTT 1050
G K WC+NTT
Sbjct: 1 GPKPWCENTT 30
Score = 22.6 bits (43), Expect(6) = 2e-038
Identities = 10/22 (45%), Positives = 11/22 (50%)
Frame = +1 / -1
Query: 442 SKTGRGMDSWC*CHHSPFLSLL 507
S+ G M SW HH P LL
Sbjct: 604 SRAGLEMSSWHHHHHKPSHPLL 539
Score = 21.7 bits (41), Expect(5) = 3e-026
Identities = 6/9 (66%), Positives = 9/9 (100%)
Frame = -1 / +1
Query: 551 KQLQNIWHI 525
+QLQ+IWH+
Sbjct: 496 QQLQDIWHL 522
>gb|AV934271.1|AV934271 AV934271 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal11l09 5', mRNA sequence
Length = 582
Score = 87.2 bits (184), Expect(2) = 4e-030
Identities = 32/66 (48%), Positives = 45/66 (68%)
Frame = -2 / +3
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDADPMWRGSKWKCLMVRWDDDVDFRQPNRISPWE 1370
G+RFK+R+E ++A E+R TG I G + D +W S W+ L VRWD+ +P+R+SPWE
Sbjct: 351 GTRFKMRFEGEEAPEQRFTGTIVGSDNLDQLWPESSWRSLKVRWDESSTIPRPDRVSPWE 530
Query: 1369 IELTSS 1352
IE SS
Sbjct: 531 IEPASS 548
Score = 65.7 bits (137), Expect(2) = 4e-030
Identities = 27/62 (43%), Positives = 41/62 (66%)
Frame = -2 / +3
Query: 1795 LFLRGDNGELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPRL 1616
+FLRG++GELR+GVRRA + + A + + +LG L HA+ TK++F +YY PR
Sbjct: 105 IFLRGESGELRVGVRRAMRQLSNIASSVISSHSMHLGVLATAWHAINTKTMFTVYYKPRT 284
Query: 1615 SQ 1610
S+
Sbjct: 285 SR 290
>gb|BJ472357.1|BJ472357 BJ472357 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal33n21 5', mRNA sequence
Length = 684
Score = 100 bits (212), Expect(2) = 1e-029
Identities = 40/98 (40%), Positives = 59/98 (60%)
Frame = -2 / +1
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDADPMWRGSKWKCLMVRWDDDVDFRQPNRISPWE 1370
G+RFK+R+E ++A E+R TG I G + D +W S W+ L VRWD+ +P+R+SPWE
Sbjct: 178 GTRFKMRFEGEEAPEQRFTGTIVGSDNLDQLWPESSWRSLKVRWDESSTIPRPDRVSPWE 357
Query: 1369 IELTSSVSGSHMSAPNAKRLKPCLPHVNPDYLVPNGSG 1256
IE SS + + AKR +P +P +P+ V G
Sbjct: 358 IEPASSPPVNPLPLSRAKRSRPNVPPASPESSVRTKEG 471
Score = 51.0 bits (105), Expect(2) = 1e-029
Identities = 24/55 (43%), Positives = 29/55 (52%)
Frame = -1 / +3
Query: 1688 WFTT*CCTCCGHQKCVPHLLQPQIKPI*IHYTIFEVYQELQSTIFCWFEVQSEI* 1524
W C C H+ V LLQ Q KP +HYTI +VY +F W EVQ E+*
Sbjct: 39 WSPCNCMACYQHKNHVHCLLQTQDKPFRVHYTI*QVYGVC*KHLFYWDEVQDEV* 203
>gb|BU987774.1|BU987774 HF15L16r HF Hordeum vulgare subsp. vulgare cDNA clone HF15L16
5-PRIME, mRNA sequence
Length = 591
Score = 94.1 bits (199), Expect(3) = 7e-026
Identities = 38/98 (38%), Positives = 58/98 (59%)
Frame = -2 / +1
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDADPMWRGSKWKCLMVRWDDDVDFRQPNRISPWE 1370
G RF++R+E ++A E+R TG I G + D +W S W+ L VRWD+ +P+R+SPW+
Sbjct: 235 GMRFRMRFEGEEAPEQRFTGTIVGSENLDQLWPESNWRSLKVRWDEPSTIPRPDRVSPWK 414
Query: 1369 IELTSSVSGSHMSAPNAKRLKPCLPHVNPDYLVPNGSG 1256
IE SS + + KR +P +P V+P+ V G
Sbjct: 415 IEPASSPPVNPLPLSRVKRPRPNVPPVSPESSVLTKEG 528
Score = 36.3 bits (73), Expect(3) = 7e-026
Identities = 14/27 (51%), Positives = 18/27 (66%)
Frame = -2 / +1
Query: 1693 NLGSLPNVAHAVATKSVFHIYYNPRLS 1613
+LG L HA+ TKS+F +YY PR S
Sbjct: 91 HLGVLATAWHAINTKSMFTVYYKPRTS 171
Score = 25.8 bits (50), Expect(3) = 7e-026
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -2 / +2
Query: 1783 GDNGELRLGVRRA 1745
G GELR+GVRRA
Sbjct: 2 GTRGELRVGVRRA 40
>gb|CA032804.1|CA032804 HX14E02r HX Hordeum vulgare subsp. vulgare cDNA clone HX14E02
5-PRIME, mRNA sequence
Length = 655
Score = 94.1 bits (199), Expect(3) = 3e-025
Identities = 38/98 (38%), Positives = 58/98 (59%)
Frame = -2 / +3
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDADPMWRGSKWKCLMVRWDDDVDFRQPNRISPWE 1370
G RF++R+E ++A E+R TG I G + D +W S W+ L VRWD+ +P+R+SPW+
Sbjct: 234 GMRFRMRFEGEEAPEQRFTGTIVGSENLDQLWPESNWRSLKVRWDEPSTIPRPDRVSPWK 413
Query: 1369 IELTSSVSGSHMSAPNAKRLKPCLPHVNPDYLVPNGSG 1256
IE SS + + KR +P +P V+P+ V G
Sbjct: 414 IEPASSPPVNPLPLSRVKRPRPNVPPVSPESSVLTKEG 527
Score = 36.3 bits (73), Expect(3) = 3e-025
Identities = 14/27 (51%), Positives = 18/27 (66%)
Frame = -2 / +3
Query: 1693 NLGSLPNVAHAVATKSVFHIYYNPRLS 1613
+LG L HA+ TKS+F +YY PR S
Sbjct: 90 HLGVLATAWHAINTKSMFTVYYKPRTS 170
Score = 23.5 bits (45), Expect(3) = 3e-025
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = -2 / +1
Query: 1774 GELRLGVRRA 1745
GELR+GVRRA
Sbjct: 10 GELRVGVRRA 39
>gb|CA031681.1|CA031681 HX10N04r HX Hordeum vulgare subsp. vulgare cDNA clone HX10N04
5-PRIME, mRNA sequence
Length = 518
Score = 73.0 bits (153), Expect(2) = 8e-023
Identities = 37/82 (45%), Positives = 49/82 (59%)
Frame = +3 / -3
Query: 1365 SISQGEILFG*RKSTSSSHRTIKHFHFEPRHMGSASPIPAMIPVHLLSEASSLSYLTLNL 1544
SIS G+ LF +S SSH T + FHFEP H+ ++ + +I LS SSL Y LNL
Sbjct: 318 SISHGDSLFWLLQSMPSSHLTSRLFHFEPVHLMGSTSLIPVIIPDRLSLTSSLPY*ILNL 139
Query: 1545 EPAENG*LKLLINFENGIMNSD 1610
P +NG +L NF+ G +NSD
Sbjct: 138 IPIQNGRFRLFRNFQYGTINSD 73
Score = 55.1 bits (114), Expect(2) = 8e-023
Identities = 21/32 (65%), Positives = 25/32 (78%)
Frame = +1 / -1
Query: 1183 EFYNPVILDLARPCGIGQIPQNQDDRFHLELG 1278
EF NP ILDLARP G+ Q+P++ DD FH ELG
Sbjct: 503 EFQNPSILDLARPWGVYQLPRSPDDHFHTELG 408
>gb|BG343130.1|BG343130 HVSMEg0002J04f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0002J04f, mRNA sequence
Length = 856
Score = 54.2 bits (112), Expect(4) = 8e-023
Identities = 18/28 (64%), Positives = 20/28 (71%)
Frame = -2 / +1
Query: 1447 SKWKCLMVRWDDDVDFRQPNRISPWEIE 1364
SKW+CLMVRWDD R+SPWEIE
Sbjct: 1 SKWRCLMVRWDDSTHCNHQRRVSPWEIE 84
Score = 49.2 bits (101), Expect(4) = 8e-023
Identities = 23/47 (48%), Positives = 30/47 (63%)
Frame = -2 / +1
Query: 1336 MSAPNAKRLKPCLPHVNPDYLVPNGSGRPDFAESAQFHKVLQGQELL 1196
+SA ++KR K P N D V +G+G D E+ FH+VLQGQELL
Sbjct: 118 LSASSSKRAKLYFPQGNLDAPVTDGNGCLDSMEAGNFHRVLQGQELL 258
Score = 43.7 bits (89), Expect(4) = 8e-023
Identities = 19/34 (55%), Positives = 21/34 (61%)
Frame = -2 / +1
Query: 1054 PLGSPRFSYRCSGFGESPRFQKVLQGQEVFHPYR 953
P S F Y SGFGES RF +VLQGQE+ R
Sbjct: 364 PQQSSEFPYHSSGFGESVRFSEVLQGQEMSQALR 465
Score = 24.0 bits (46), Expect(4) = 8e-023
Identities = 12/34 (35%), Positives = 14/34 (41%)
Frame = -2 / +1
Query: 772 GDPKMSPFEFGHFHLNKNEDRRAMFVHAGGIGGT 671
G P SP+E G KN R A + GT
Sbjct: 727 GSPGFSPWEMGEGKREKNIGRAPTGAKAQALTGT 828
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 939,377,046
Number of Sequences: 312970
Number of extensions: 16317669
Number of successful extensions: 77019
Number of sequences better than 1.0e-020: 12
length of database: 58,378,179
effective HSP length: 54
effective length of database: 41,477,799
effective search space used: 22563922656
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)