TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441100.2.1
(646 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ759822.1|BQ759822 EBpi05_SQ004_B16_R pistil, 8 DPA, no... 142 2e-033
gb|BJ462342.1|BJ462342 BJ462342 K. Sato unpublished cDNA li... 133 1e-030
gb|BJ466593.1|BJ466593 BJ466593 K. Sato unpublished cDNA li... 133 1e-030
gb|BU996676.1|BU996676 HM14G05r HM Hordeum vulgare subsp. v... 133 1e-030
gb|CA006313.1|CA006313 HU14I15u HU Hordeum vulgare subsp. v... 133 1e-030
gb|BF630195.2|BF630195 HVSMEb0008E12f Hordeum vulgare seedl... 119 1e-026
gb|BQ665583.1|BQ665583 HX04E07u HX Hordeum vulgare subsp. v... 119 1e-026
gb|CA022047.1|CA022047 HZ42A04r HZ Hordeum vulgare subsp. v... 119 1e-026
gb|BG368552.1|BG368552 HVSMEi0019L05f Hordeum vulgare 20 DA... 119 2e-026
gb|AV927421.1|AV927421 AV927421 K. Sato unpublished cDNA li... 118 2e-026
gb|AV928242.1|AV928242 AV928242 K. Sato unpublished cDNA li... 118 2e-026
gb|BJ467826.1|BJ467826 BJ467826 K. Sato unpublished cDNA li... 118 2e-026
gb|BM374192.2|BM374192 EBma03_SQ003_O16_R maternal, 8 DPA, ... 118 2e-026
gb|AV927437.1|AV927437 AV927437 K. Sato unpublished cDNA li... 111 3e-024
gb|BI956767.1|BI956767 HVSMEn0005D24f Hordeum vulgare rachi... 111 4e-024
gb|BQ759816.1|BQ759816 EBpi05_SQ004_A16_R pistil, 8 DPA, no... 105 3e-022
>gb|BQ759822.1|BQ759822 EBpi05_SQ004_B16_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ004_B16 5', mRNA sequence
Length = 646
Score = 142 bits (305), Expect = 2e-033
Identities = 57/69 (82%), Positives = 62/69 (89%)
Frame = +3 / +1
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
VEACKKATGA+IKV+YL RRPGDYAEVYS+PSKI ELNWTAQ+TDL +SLA AWKWQKA
Sbjct: 430 VEACKKATGATIKVDYLDRRPGDYAEVYSNPSKIRDELNWTAQHTDLRESLATAWKWQKA 609
Query: 189 HPNGYGPA* 215
HP GYG A*
Sbjct: 610 HPGGYGSA* 636
Score = 114 bits (244), Expect = 4e-025
Identities = 52/72 (72%), Positives = 56/72 (77%)
Frame = -1 / -3
Query: 217 NQAGPYPFGCAFCHFHAWARLWPRSVYWAVQLSSMWIFDGSLYTSA*SPGLRARYSTLIE 38
+QA PYP GCAFCHF A ARL RSV WAVQ SS I DG LYTSA*SPGLR+R STL+
Sbjct: 638 DQADPYPPGCAFCHFQAVARLSRRSVCWAVQFSSSRILDGLLYTSA*SPGLRSR*STLMV 459
Query: 37 APVAFLQASTRA 2
APVAFL AST +
Sbjct: 458 APVAFLHASTNS 423
>gb|BJ462342.1|BJ462342 BJ462342 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak24n03 3', mRNA sequence
Length = 699
Score = 133 bits (284), Expect = 1e-030
Identities = 48/66 (72%), Positives = 60/66 (90%)
Frame = +3 / -2
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
V+ACK+ATG IK+EYL+RRPGDYAEV+SDP+KI+ ELNWTAQ+TDL +SL+ AW+WQK+
Sbjct: 464 VDACKQATGVDIKIEYLSRRPGDYAEVFSDPTKINNELNWTAQHTDLKESLSVAWRWQKS 285
Query: 189 HPNGYG 206
HP GYG
Sbjct: 284 HPRGYG 267
>gb|BJ466593.1|BJ466593 BJ466593 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32o13 3', mRNA sequence
Length = 678
Score = 133 bits (284), Expect = 1e-030
Identities = 48/66 (72%), Positives = 60/66 (90%)
Frame = +3 / -2
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
V+ACK+ATG IK+EYL+RRPGDYAEV+SDP+KI+ ELNWTAQ+TDL +SL+ AW+WQK+
Sbjct: 467 VDACKQATGVDIKIEYLSRRPGDYAEVFSDPTKINNELNWTAQHTDLKESLSVAWRWQKS 288
Query: 189 HPNGYG 206
HP GYG
Sbjct: 287 HPRGYG 270
>gb|BU996676.1|BU996676 HM14G05r HM Hordeum vulgare subsp. vulgare cDNA clone HM14G05
5-PRIME, mRNA sequence
Length = 536
Score = 133 bits (284), Expect = 1e-030
Identities = 48/66 (72%), Positives = 60/66 (90%)
Frame = +3 / +1
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
V+ACK+ATG IK+EYL+RRPGDYAEV+SDP+KI+ ELNWTAQ+TDL +SL+ AW+WQK+
Sbjct: 52 VDACKQATGVDIKIEYLSRRPGDYAEVFSDPTKINNELNWTAQHTDLKESLSVAWRWQKS 231
Query: 189 HPNGYG 206
HP GYG
Sbjct: 232 HPRGYG 249
>gb|CA006313.1|CA006313 HU14I15u HU Hordeum vulgare subsp. vulgare cDNA clone HU14I15
3-PRIME, mRNA sequence
Length = 550
Score = 133 bits (284), Expect = 1e-030
Identities = 48/66 (72%), Positives = 60/66 (90%)
Frame = +3 / -1
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
V+ACK+ATG IK+EYL+RRPGDYAEV+SDP+KI+ ELNWTAQ+TDL +SL+ AW+WQK+
Sbjct: 448 VDACKQATGVDIKIEYLSRRPGDYAEVFSDPTKINNELNWTAQHTDLKESLSVAWRWQKS 269
Query: 189 HPNGYG 206
HP GYG
Sbjct: 268 HPRGYG 251
>gb|BF630195.2|BF630195 HVSMEb0008E12f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0008E12f, mRNA sequence
Length = 497
Score = 119 bits (255), Expect = 1e-026
Identities = 45/65 (69%), Positives = 54/65 (83%)
Frame = +3 / +2
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
VEACKKATG IKV+Y RRPGDYAEVYS+P++I+ ELNWTAQ+T+L +SL AW WQK
Sbjct: 152 VEACKKATGVDIKVDYFPRRPGDYAEVYSNPARINRELNWTAQHTELQESLRVAWTWQKK 331
Query: 189 HPNGY 203
H +GY
Sbjct: 332 HRSGY 346
>gb|BQ665583.1|BQ665583 HX04E07u HX Hordeum vulgare subsp. vulgare cDNA clone HX04E07
3-PRIME, mRNA sequence
Length = 516
Score = 119 bits (255), Expect = 1e-026
Identities = 45/65 (69%), Positives = 54/65 (83%)
Frame = +3 / -2
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
VEACKKATG IKV+Y RRPGDYAEVYS+P++I+ ELNWTAQ+T+L +SL AW WQK
Sbjct: 329 VEACKKATGVDIKVDYFPRRPGDYAEVYSNPARINRELNWTAQHTELQESLRVAWTWQKK 150
Query: 189 HPNGY 203
H +GY
Sbjct: 149 HRSGY 135
>gb|CA022047.1|CA022047 HZ42A04r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ42A04
5-PRIME, mRNA sequence
Length = 533
Score = 119 bits (255), Expect = 1e-026
Identities = 45/65 (69%), Positives = 54/65 (83%)
Frame = +3 / +1
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
VEACKKATG IKV+Y RRPGDYAEVYS+P++I+ ELNWTAQ+T+L +SL AW WQK
Sbjct: 205 VEACKKATGVDIKVDYFPRRPGDYAEVYSNPARINRELNWTAQHTELQESLRVAWTWQKK 384
Query: 189 HPNGY 203
H +GY
Sbjct: 385 HRSGY 399
>gb|BG368552.1|BG368552 HVSMEi0019L05f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0019L05f, mRNA sequence
Length = 618
Score = 119 bits (254), Expect = 2e-026
Identities = 45/65 (69%), Positives = 53/65 (81%)
Frame = +3 / +2
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
VEACKKATG IKV+Y RRPGDYAEVYS+P++I+ ELNWTAQ+T+L +SL W WQK
Sbjct: 377 VEACKKATGVDIKVDYFPRRPGDYAEVYSNPARINRELNWTAQHTELPESLRGRWTWQKN 556
Query: 189 HPNGY 203
H NGY
Sbjct: 557 HRNGY 571
>gb|AV927421.1|AV927421 AV927421 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd2a02 3', mRNA sequence
Length = 691
Score = 118 bits (253), Expect = 2e-026
Identities = 45/65 (69%), Positives = 53/65 (81%)
Frame = +3 / -1
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
VEACKKATG IKV Y RRPGDYAEVYS+P++I+ ELNWTAQ+T+L +SL AW WQK
Sbjct: 319 VEACKKATGVDIKVHYFPRRPGDYAEVYSNPARINRELNWTAQHTELQESLRVAWTWQKK 140
Query: 189 HPNGY 203
H +GY
Sbjct: 139 HRSGY 125
>gb|AV928242.1|AV928242 AV928242 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd27i15 3', mRNA sequence
Length = 678
Score = 118 bits (253), Expect = 2e-026
Identities = 45/65 (69%), Positives = 53/65 (81%)
Frame = +3 / -1
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
VEACKKATG IKV Y RRPGDYAEVYS+P++I+ ELNWTAQ+T+L +SL AW WQK
Sbjct: 318 VEACKKATGVDIKVHYFPRRPGDYAEVYSNPARINRELNWTAQHTELQESLRVAWTWQKK 139
Query: 189 HPNGY 203
H +GY
Sbjct: 138 HRSGY 124
>gb|BJ467826.1|BJ467826 BJ467826 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags29p06 3', mRNA sequence
Length = 658
Score = 118 bits (253), Expect = 2e-026
Identities = 45/65 (69%), Positives = 53/65 (81%)
Frame = +3 / -2
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
VEACKKATG IKV Y RRPGDYAEVYS+P++I+ ELNWTAQ+T+L +SL AW WQK
Sbjct: 321 VEACKKATGVDIKVHYFPRRPGDYAEVYSNPARINRELNWTAQHTELQESLRVAWTWQKK 142
Query: 189 HPNGY 203
H +GY
Sbjct: 141 HRSGY 127
>gb|BM374192.2|BM374192 EBma03_SQ003_O16_R maternal, 8 DPA, no treatment, cv Optic, EBma03
Hordeum vulgare subsp. vulgare cDNA clone
EBma03_SQ003_O16 5', mRNA sequence
Length = 526
Score = 118 bits (253), Expect = 2e-026
Identities = 45/65 (69%), Positives = 53/65 (81%)
Frame = +3 / +2
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
VEACKKATG IKV Y RRPGDYAEVYS+P++I+ ELNWTAQ+T+L +SL AW WQK
Sbjct: 179 VEACKKATGVDIKVHYFPRRPGDYAEVYSNPARINRELNWTAQHTELQESLRVAWTWQKK 358
Query: 189 HPNGY 203
H +GY
Sbjct: 359 HRSGY 373
>gb|AV927437.1|AV927437 AV927437 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd2b02 3', mRNA sequence
Length = 532
Score = 111 bits (238), Expect = 3e-024
Identities = 44/65 (67%), Positives = 52/65 (80%)
Frame = +3 / -2
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
VEACKKATG IKV Y RRPGDYAEVYS+P++I+ ELNWTAQ+T+L +SL A WQK
Sbjct: 249 VEACKKATGVDIKVHYFPRRPGDYAEVYSNPARINRELNWTAQHTELQESLRVA*TWQKK 70
Query: 189 HPNGY 203
H +GY
Sbjct: 69 HRSGY 55
>gb|BI956767.1|BI956767 HVSMEn0005D24f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005D24f, mRNA sequence
Length = 1150
Score = 111 bits (237), Expect = 4e-024
Identities = 42/62 (67%), Positives = 51/62 (82%)
Frame = +3 / +1
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLAQAWKWQKA 188
VEACKKATG IKV+Y RRPGDYAEV+S+P++I+ ELNWTAQ+T+L +SL AW WQK
Sbjct: 292 VEACKKATGVDIKVDYFPRRPGDYAEVFSNPARINRELNWTAQHTELQESLTVAWTWQKK 471
Query: 189 HP 194
P
Sbjct: 472 TP 477
>gb|BQ759816.1|BQ759816 EBpi05_SQ004_A16_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ004_A16 5', mRNA sequence
Length = 587
Score = 105 bits (223), Expect = 3e-022
Identities = 43/52 (82%), Positives = 48/52 (92%)
Frame = +3 / +1
Query: 9 VEACKKATGASIKVEYLARRPGDYAEVYSDPSKIHMELNWTAQYTDLGQSLA 164
VEACKKATGA+IKV+YL RRPGDYAEVYS+PSKI ELNWTAQ+TDL +SLA
Sbjct: 430 VEACKKATGATIKVDYLDRRPGDYAEVYSNPSKIRDELNWTAQHTDLRESLA 585
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 280,589,216
Number of Sequences: 312970
Number of extensions: 4452645
Number of successful extensions: 48673
Number of sequences better than 1.0e-020: 20
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6913923567
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)