TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441058.2.1
(1000 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ462645.1|BJ462645 BJ462645 K. Sato unpublished cDNA li... 346 e-110
gb|AV918442.1|AV918442 AV918442 K. Sato unpublished cDNA li... 240 4e-063
gb|BQ663683.1|BQ663683 HU03J08u HU Hordeum vulgare subsp. v... 227 1e-061
gb|BQ465414.1|BQ465414 HU03J08r HU Hordeum vulgare subsp. v... 124 4e-044
>gb|BJ462645.1|BJ462645 BJ462645 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags29k09 5', mRNA sequence
Length = 626
Score = 346 bits (749), Expect(4) = e-110
Identities = 142/158 (89%), Positives = 151/158 (95%)
Frame = +1 / +1
Query: 142 VSSIEALHSATKALPPDFVRLDQDILSPRAGKKELYTYQTLDFWEQIKTPGMSLRCSGLY 321
VSS EAL SA KA+P D+VRLDQDILSP AGKKELYTY+TLDFWEQIKTPGMSLRCSGLY
Sbjct: 151 VSSFEALQSAMKAIPADYVRLDQDILSPLAGKKELYTYETLDFWEQIKTPGMSLRCSGLY 330
Query: 322 LSQFRHTSPHLLASGDGKRTATIVGDVYIHPSAKVHPTSKIGPNVSISANARVGAGARLI 501
LSQFRHTSPHLLASGDGK+ ATI+GDVYIHPSAKVH T+KIGPNVSISANAR+GAGARLI
Sbjct: 331 LSQFRHTSPHLLASGDGKKGATIIGDVYIHPSAKVHLTAKIGPNVSISANARIGAGARLI 510
Query: 502 NCIILDDVEIMENAVVIHSIVGWKSSIGKWSRVQGEGD 615
NCIILDDVEIMENAVVIHSIVGWKS++GKWSRVQGEGD
Sbjct: 511 NCIILDDVEIMENAVVIHSIVGWKSTVGKWSRVQGEGD 624
Score = 275 bits (594), Expect(4) = 9e-085
Identities = 121/167 (72%), Positives = 128/167 (76%)
Frame = -3 / -1
Query: 614 SPSPCTRDHFPMDDFHPTIECXXXXXXXXXXXXXIMQLISLAPAPTRAFADIETFGPIFE 435
SPSPCTRDHFP DFHPTIEC +MQLISLAPAP RAFADIET GPIF
Sbjct: 623 SPSPCTRDHFPTVDFHPTIECITTAFSIISTSSRMMQLISLAPAPMRAFADIETLGPIFA 444
Query: 434 VGCTLADGWI*TSPTIVAVLLPSPEAKR*GEVWRN*ERYNPEHLKDIPGVLICSQKSSVW 255
V T ADGW+ TSP IVA LPSPEAKR*GEVWRN ERY PEHLKDIPGVLICSQKSSV
Sbjct: 443 VR*TFADGWMYTSPIIVAPFLPSPEAKR*GEVWRNWERYRPEHLKDIPGVLICSQKSSVS 264
Query: 254 YVYSSFFPARGDKMS*SNLTKSGGSALVAE*RASMLDTKKTNLLCLF 114
YV SSFFPARGDK+S*SNLT S G A +A+*RAS LDT++ L F
Sbjct: 263 YV*SSFFPARGDKIS*SNLT*SAGIAFIAD*RASKLDTRRKLALSFF 123
Score = 239 bits (517), Expect(2) = 6e-067
Identities = 107/158 (67%), Positives = 121/158 (76%)
Frame = -2 / -3
Query: 615 ITFALYT*PFSNG*FPPYN*MYNNSIFHNFNIIQNYAIDKPGTSSNACIC*YRDIWANL* 436
+TF LYT*PF * PPY *MYNN I HNFNIIQN AIDKPG+ SNACIC*Y DI ANL
Sbjct: 624 VTFTLYT*PFPYC*LPPYY*MYNNCILHNFNIIQNDAIDKPGSCSNACIC*Y*DIGANLC 445
Query: 435 SRMHLGRWMDIDIANNSGSSFAIS*G*KMRRGMAKLREI*PRASQRHPWGFDLFPEIKCL 256
S ++L RWMD+ + NSG+ FAIS G +MRRGMAKL EI* RASQRHPW DLFP+IKCL
Sbjct: 444 S*VNLCRWMDVHVTYNSGTLFAISRGQEMRRGMAKLGEI*TRASQRHPWSLDLFPKIKCL 265
Query: 255 VCVQLLLSRSRG*NVLI*PNKVWWKRLSC*MKSFNARY 142
VCV+ LLS R *++LI*PN + W L C +KSF A Y
Sbjct: 264 VCVKFLLSC*RR*DILI*PNIICWNCLHCGLKSFKAGY 151
Score = 192 bits (414), Expect(3) = 7e-055
Identities = 99/158 (62%), Positives = 107/158 (67%)
Frame = +2 / +2
Query: 140 LYLALKLFIQQLRRFHQTLLG*IKTFYPLERERRSCTHTRHLISGNRSKPQGCL*DALGY 319
+Y ALKLF Q R+F Q +LG*IK Y L +ERR+ THTRHLI GNRS+ QGCL*DAL Y
Sbjct: 149 VYPALKLFSPQ*RQFQQIMLG*IKISYLL*QERRNFTHTRHLIFGNRSRLQGCL*DALVY 328
Query: 320 ISLSFAIPLLIF*PQEMAKELPLLLAMSISIHLPRCILLQRLAQMSLYQQMHALELVPGL 499
IS SFAIPLLI P EMAK +PLL SIHL R LQRLA MS YQQMHALE PGL
Sbjct: 329 ISPSFAIPLLISWPLEMAKRVPLL*VTCTSIHLQRFT*LQRLAPMSQYQQMHALEQEPGL 508
Query: 500 SIA*FXXXXXXXXXXXXYIQL*GGNHPLENGHVYRAKV 613
SIA F YIQ *GG+ NGHVYR KV
Sbjct: 509 SIASFWMMLKLWRMQLLYIQ**GGSQQ*GNGHVYRVKV 622
Score = 162 bits (348), Expect(4) = 6e-047
Identities = 80/121 (66%), Positives = 86/121 (71%)
Frame = -1 / -2
Query: 514 ELCN**AWHQLQRVHLLI*RHLGQSLK*DAPWQMDGYRHRQQ*WQFFCHLLRLKDEERYG 335
E CN**AW LQ VHLLI RH GQSL+ P QMDG *W FCHL R +DEERYG
Sbjct: 523 E*CN**AWLLLQCVHLLILRHWGQSLQLGEPLQMDGCTRHL**WHPFCHLQRPRDEERYG 344
Query: 334 ETERDITQSISKTSLGF*SVPRNQVSGMCTAPSFPLEGIKCLDLT*QSLVEAP*LLNEEL 155
ET RDI QSISKTSL *SVP+NQVS MC PSF LE I+ LDLT* +L+E P L EEL
Sbjct: 343 ETGRDIDQSISKTSLES*SVPKNQVSRMCKVPSFLLEEIRYLDLT*HNLLELPSLRTEEL 164
Query: 154 Q 152
Q
Sbjct: 163 Q 161
Score = 158 bits (339), Expect(3) = 9e-046
Identities = 66/110 (60%), Positives = 76/110 (69%)
Frame = +3 / +3
Query: 186 TRLC*VRSRHFIPSSGKEGAVHIPDT*FLGTDQNPRDVFEMLWVIXXXXXXXXXXXXXXR 365
+RLC*VRSR+ I SS KEG +HI DT*FLGTDQ+ RDVFEMLW I
Sbjct: 195 SRLC*VRSRYLISSSRKEGTLHIRDT*FLGTDQDSRDVFEMLWSISLPVSPYLSSSLGLW 374
Query: 366 RWQKNCHYCWRCLYPSICQGASYFKDWPKCLYISKCTRWSWCQAYQLHNS 515
RWQK CHY ++PSIC+G+ KDWP+CL ISKCT WS QAYQLH+S
Sbjct: 375 RWQKGCHYYR*RVHPSICKGSPNCKDWPQCLNISKCTHWSRSQAYQLHHS 524
Score = 53.8 bits (111), Expect(4) = e-110
Identities = 22/24 (91%), Positives = 24/24 (100%)
Frame = +2 / +1
Query: 62 YIFTPDILSAIEDVLKQKKDRANL 133
YIFTP+IL+AIEDVLKQKKDRANL
Sbjct: 73 YIFTPNILNAIEDVLKQKKDRANL 144
Score = 46.0 bits (94), Expect(4) = 9e-085
Identities = 19/25 (76%), Positives = 21/25 (84%)
Frame = -2 / -1
Query: 138 KNKFALSFFCFKTSSMALRISGVNI 64
+ K ALSFFCFKTSS+AL I GVNI
Sbjct: 149 RRKLALSFFCFKTSSIALSILGVNI 75
Score = 35.4 bits (71), Expect(2) = 6e-067
Identities = 18/35 (51%), Positives = 20/35 (57%)
Frame = -1 / -3
Query: 133 QICSVFFLF*DILNGAKDIGSKYISPQFMRSLTKS 29
QI S+F LF DIL+ K IGSKYI KS
Sbjct: 144 QISSIFLLFQDILDCIKYIGSKYIDSAIYEITHKS 40
Score = 32.7 bits (65), Expect(3) = 9e-046
Identities = 15/21 (71%), Positives = 16/21 (76%)
Frame = +3 / +2
Query: 63 IYLLPISLAPLRMS*NRKKTE 125
IYLLPI L RMS*NR+K E
Sbjct: 74 IYLLPIYLMQSRMS*NRRKIE 136
Score = 32.7 bits (65), Expect(3) = 7e-055
Identities = 14/27 (51%), Positives = 20/27 (74%)
Frame = +1 / +3
Query: 52 IAEIYIYSRYP*RH*GCLKTEKRQSKF 132
+ +YIYS+Y * + GCL+TE+R S F
Sbjct: 63 LRSLYIYSQYT*CNRGCLETEER*S*F 143
Score = 32.2 bits (64), Expect(4) = 6e-047
Identities = 14/21 (66%), Positives = 15/21 (71%)
Frame = -1 / -2
Query: 613 HLRPVHVTIFQWMISTLQLNV 551
HL PVHVTI + STL LNV
Sbjct: 622 HLHPVHVTISLLLTSTLLLNV 560
Score = 31.8 bits (63), Expect(3) = 9e-046
Identities = 14/21 (66%), Positives = 16/21 (76%)
Frame = +3 / +3
Query: 552 TFNCRVEIIHWKMVTCTGRR* 614
TFN RVE+ +MVTCTG R*
Sbjct: 561 TFNSRVEVNSREMVTCTG*R* 623
Score = 30.4 bits (60), Expect(4) = e-110
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +1 / +1
Query: 1 ELLHYTEKPET 33
ELLHYTEKPET
Sbjct: 10 ELLHYTEKPET 42
Score = 28.1 bits (55), Expect(3) = 7e-055
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = +2 / +3
Query: 17 RKSRRLCE*SHKLR 58
RK+R CE*SHKLR
Sbjct: 27 RKTRDFCE*SHKLR 68
Score = 26.3 bits (51), Expect(4) = 9e-085
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -1 / -1
Query: 79 IGSKYISPQFMRSLTKSPAF 20
+G +PQFMRSLTK F
Sbjct: 89 LGVNI*TPQFMRSLTKVSGF 30
Score = 26.3 bits (51), Expect(4) = e-110
Identities = 11/19 (57%), Positives = 11/19 (57%)
Frame = +3 / +1
Query: 21 KAGDFVSDLINCGDIYLLP 77
K FVSDLINCG P
Sbjct: 31 KPETFVSDLINCGVYIFTP 87
Score = 25.8 bits (50), Expect(4) = 6e-047
Identities = 14/23 (60%), Positives = 15/23 (65%)
Frame = -3 / -2
Query: 131 NLLCLFSVLRHPQWR*GYRE*IY 63
N L L SV RHP+ * Y E*IY
Sbjct: 142 N*LYLSSVSRHPRLH*VYWE*IY 74
Score = 24.9 bits (48), Expect(4) = 9e-085
Identities = 10/11 (90%), Positives = 11/11 (100%)
Frame = -3 / -1
Query: 35 KVSGFSV*CSS 3
KVSGFSV*C+S
Sbjct: 44 KVSGFSV*CNS 12
Score = 24.0 bits (46), Expect(4) = 6e-047
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = -3 / -3
Query: 59 SAIYEITHK 33
SAIYEITHK
Sbjct: 69 SAIYEITHK 43
>gb|AV918442.1|AV918442 AV918442 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags21b04 3', mRNA sequence
Length = 587
Score = 240 bits (518), Expect(2) = 4e-063
Identities = 103/124 (83%), Positives = 109/124 (87%)
Frame = +1 / -1
Query: 382 ATIVGDVYIHPSAKVHPTSKIGPNVSISANARVGAGARLINCIILDDVEIMENAVVIHSI 561
ATI+G VYIHP AKVH T+KIGPNVSISANAR+GAGARLINCIILDDVEIMENAVVIHSI
Sbjct: 581 ATIIGXVYIHPXAKVHLTAKIGPNVSISANARIGAGARLINCIILDDVEIMENAVVIHSI 402
Query: 562 VGWKSSIGKWSRVQGEGDHNAKLGITILGEAXXXXXXXXXXNSIVLPNKTLNVSVQEEII 741
VGWKS++GKWSRVQGEGDHNAKLGITILGEA NSIVLPNKTLN SVQ+EII
Sbjct: 401 VGWKSTVGKWSRVQGEGDHNAKLGITILGEAVDVEDEVVVTNSIVLPNKTLNASVQDEII 222
Query: 742 L**P 753
L* P
Sbjct: 221 L*YP 210
Score = 174 bits (375), Expect = 6e-043
Identities = 77/125 (61%), Positives = 82/125 (65%)
Frame = -3 / +1
Query: 755 DGYYRMISSWTLTLRVLFGXXXXXXXXXXXXXXXXSPRIVMPSLAL*SPSPCTRDHFPMD 576
+GYYRMISS TL LRVLFG SPR+V+PSLAL SPSPCTRDHFP
Sbjct: 208 NGYYRMISSCTLALRVLFGSTMLLVTTTSSSTSTASPRMVIPSLALWSPSPCTRDHFPTV 387
Query: 575 DFHPTIECXXXXXXXXXXXXXIMQLISLAPAPTRAFADIETFGPIFEVGCTLADGWI*TS 396
DFHPTIEC +MQLISLAPAP RAFADIET GPIF V T A GW+ TS
Sbjct: 388 DFHPTIECITTAFSIISTSSRMMQLISLAPAPMRAFADIETLGPIFAVR*TFAXGWMYTS 567
Query: 395 PTIVA 381
P IVA
Sbjct: 568 PIIVA 582
Score = 169 bits (364), Expect(2) = 3e-045
Identities = 75/113 (66%), Positives = 82/113 (72%)
Frame = -2 / +3
Query: 717 IESFIWEHDAVXXXXXXXXXNGFTKNSNAKFGIVITFALYT*PFSNG*FPPYN*MYNNSI 538
IESFIWEHDAV NGFTKN + KFGIV+TF LYT*PF * PPY *MYNN I
Sbjct: 246 IESFIWEHDAVSYNYLIFNINGFTKNGDTKFGIVVTFTLYT*PFPYC*LPPYY*MYNNCI 425
Query: 537 FHNFNIIQNYAIDKPGTSSNACIC*YRDIWANL*SRMHLGRWMDIDIANNSGS 379
HNFNIIQN AIDKPG+ SNACIC*Y DI ANL S ++L WMD+ NSG+
Sbjct: 426 LHNFNIIQNDAIDKPGSCSNACIC*Y*DIGANLCS*VNLCXWMDVHXTYNSGT 584
Score = 119 bits (255), Expect(2) = 1e-042
Identities = 51/68 (75%), Positives = 58/68 (85%)
Frame = +3 / -3
Query: 552 TFNCRVEIIHWKMVTCTGRR*SQCQTWHYYSW*SR*C*R*SSCS*QHRAPK*NSQCQCPR 731
TFN RVE+ +MVTCTG R* QCQTW+++SW*SR*C*R* SC+*QHRAPK*NSQCQ R
Sbjct: 411 TFNSRVEVNSREMVTCTG*R*PQCQTWYHHSW*SR*C*R*GSCN*QHRAPK*NSQCQRAR 232
Query: 732 GDHPIITI 755
DHPII+I
Sbjct: 231 *DHPIISI 208
Score = 90.4 bits (191), Expect(2) = 2e-024
Identities = 47/83 (56%), Positives = 49/83 (59%)
Frame = +2 / -2
Query: 380 LPLLLAMSISIHLPRCILLQRLAQMSLYQQMHALELVPGLSIA*FXXXXXXXXXXXXYIQ 559
+PLL SIH R LQRLA MS YQQMHALE PGLSIA F YIQ
Sbjct: 583 VPLL*VXCTSIHXQRFT*LQRLAPMSQYQQMHALEQEPGLSIASFWMMLKLWRMQLLYIQ 404
Query: 560 L*GGNHPLENGHVYRAKVITMPN 628
*GG+ NGHVYR KV TMPN
Sbjct: 403 **GGSQQ*GNGHVYRVKVTTMPN 335
Score = 73.9 bits (155), Expect(2) = 1e-042
Identities = 27/45 (60%), Positives = 33/45 (73%)
Frame = +3 / -3
Query: 381 CHYCWRCLYPSICQGASYFKDWPKCLYISKCTRWSWCQAYQLHNS 515
CHY ++PS C+G+ KDWP+CL ISKCT WS QAYQLH+S
Sbjct: 582 CHYYR*XVHPSXCKGSPNCKDWPQCLNISKCTHWSRSQAYQLHHS 448
Score = 42.3 bits (86), Expect(2) = 2e-024
Identities = 18/23 (78%), Positives = 18/23 (78%)
Frame = +2 / -2
Query: 686 TASCSQIKLSMSVSKRRSSYNNH 754
TASCSQIKLSM K RSSYN H
Sbjct: 277 TASCSQIKLSMPACKMRSSYNIH 209
Score = 32.7 bits (65), Expect(2) = 3e-045
Identities = 16/21 (76%), Positives = 16/21 (76%)
Frame = -1 / +2
Query: 754 MVIIG*SPLGH*H*EFYLGAR 692
M IIG*S L H*EFYLGAR
Sbjct: 209 MDIIG*SHLARWH*EFYLGAR 271
Score = 22.1 bits (42), Expect(2) = 4e-063
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +2 / -3
Query: 821 PCNVTFH*IEIISLH 865
P +V FH*I +ISL+
Sbjct: 126 PPHVCFH*IGVISLY 82
>gb|BQ663683.1|BQ663683 HU03J08u HU Hordeum vulgare subsp. vulgare cDNA clone HU03J08
3-PRIME, mRNA sequence
Length = 619
Score = 227 bits (490), Expect(3) = 1e-061
Identities = 98/116 (84%), Positives = 103/116 (88%)
Frame = +1 / -1
Query: 406 IHPSAKVHPTSKIGPNVSISANARVGAGARLINCIILDDVEIMENAVVIHSIVGWKSSIG 585
IHPSAKVH T+KIGPNVSISANAR+GAGARLINCIILDDVEIMENAVVIHSIVGWKS++G
Sbjct: 619 IHPSAKVHLTAKIGPNVSISANARIGAGARLINCIILDDVEIMENAVVIHSIVGWKSTVG 440
Query: 586 KWSRVQGEGDHNAKLGITILGEAXXXXXXXXXXNSIVLPNKTLNVSVQEEIIL**P 753
KWSRVQGEGDHNAKLGITILGEA NSIVLPNKTLN SVQ+EIIL* P
Sbjct: 439 KWSRVQGEGDHNAKLGITILGEAVDVEDEVVVTNSIVLPNKTLNASVQDEIIL*YP 272
Score = 166 bits (358), Expect = 1e-040
Identities = 72/116 (62%), Positives = 76/116 (65%)
Frame = -3 / +3
Query: 755 DGYYRMISSWTLTLRVLFGXXXXXXXXXXXXXXXXSPRIVMPSLAL*SPSPCTRDHFPMD 576
+GYYRMISS TL LRVLFG SPR+V+PSLAL SPSPCTRDHFP
Sbjct: 270 NGYYRMISSCTLALRVLFGSTMLLVTTTSSSTSTASPRMVIPSLALWSPSPCTRDHFPTV 449
Query: 575 DFHPTIECXXXXXXXXXXXXXIMQLISLAPAPTRAFADIETFGPIFEVGCTLADGW 408
DFHPTIEC +MQLISLAPAP RAFADIET GPIF V T ADGW
Sbjct: 450 DFHPTIECITTAFSIISTSSRMMQLISLAPAPMRAFADIETLGPIFAVR*TFADGW 617
Score = 165 bits (354), Expect(3) = 5e-044
Identities = 73/104 (70%), Positives = 78/104 (75%)
Frame = -2 / +2
Query: 717 IESFIWEHDAVXXXXXXXXXNGFTKNSNAKFGIVITFALYT*PFSNG*FPPYN*MYNNSI 538
IESFIWEHDAV NGFTKN + KFGIV+TF LYT*PF * PPY *MYNN I
Sbjct: 308 IESFIWEHDAVSYNYLIFNINGFTKNGDTKFGIVVTFTLYT*PFPYC*LPPYY*MYNNCI 487
Query: 537 FHNFNIIQNYAIDKPGTSSNACIC*YRDIWANL*SRMHLGRWMD 406
HNFNIIQN AIDKPG+ SNACIC*Y DI ANL S ++L RWMD
Sbjct: 488 LHNFNIIQNDAIDKPGSCSNACIC*Y*DIGANLCS*VNLCRWMD 619
Score = 119 bits (255), Expect(2) = 4e-041
Identities = 51/68 (75%), Positives = 58/68 (85%)
Frame = +3 / -3
Query: 552 TFNCRVEIIHWKMVTCTGRR*SQCQTWHYYSW*SR*C*R*SSCS*QHRAPK*NSQCQCPR 731
TFN RVE+ +MVTCTG R* QCQTW+++SW*SR*C*R* SC+*QHRAPK*NSQCQ R
Sbjct: 473 TFNSRVEVNSREMVTCTG*R*PQCQTWYHHSW*SR*C*R*GSCN*QHRAPK*NSQCQRAR 294
Query: 732 GDHPIITI 755
DHPII+I
Sbjct: 293 *DHPIISI 270
Score = 88.6 bits (187), Expect(2) = 7e-024
Identities = 45/74 (60%), Positives = 46/74 (62%)
Frame = +2 / -2
Query: 407 SIHLPRCILLQRLAQMSLYQQMHALELVPGLSIA*FXXXXXXXXXXXXYIQL*GGNHPLE 586
SIHL R LQRLA MS YQQMHALE PGLSIA F YIQ *GG+
Sbjct: 618 SIHLQRFT*LQRLAPMSQYQQMHALEQEPGLSIASFWMMLKLWRMQLLYIQ**GGSQQ*G 439
Query: 587 NGHVYRAKVITMPN 628
NGHVYR KV TMPN
Sbjct: 438 NGHVYRVKVTTMPN 397
Score = 68.9 bits (144), Expect(2) = 4e-041
Identities = 25/36 (69%), Positives = 29/36 (80%)
Frame = +3 / -3
Query: 408 PSICQGASYFKDWPKCLYISKCTRWSWCQAYQLHNS 515
PSIC+G+ KDWP+CL ISKCT WS QAYQLH+S
Sbjct: 617 PSICKGSPNCKDWPQCLNISKCTHWSRSQAYQLHHS 510
Score = 42.3 bits (86), Expect(2) = 7e-024
Identities = 18/23 (78%), Positives = 18/23 (78%)
Frame = +2 / -2
Query: 686 TASCSQIKLSMSVSKRRSSYNNH 754
TASCSQIKLSM K RSSYN H
Sbjct: 339 TASCSQIKLSMPACKMRSSYNIH 271
Score = 32.7 bits (65), Expect(3) = 5e-044
Identities = 16/21 (76%), Positives = 16/21 (76%)
Frame = -1 / +1
Query: 754 MVIIG*SPLGH*H*EFYLGAR 692
M IIG*S L H*EFYLGAR
Sbjct: 271 MDIIG*SHLARWH*EFYLGAR 333
Score = 26.3 bits (51), Expect(3) = 1e-061
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = +2 / -3
Query: 938 CKKCTNR*HHFL*E 979
C+ CTNR HF *E
Sbjct: 83 CRNCTNRSQHFY*E 42
Score = 22.6 bits (43), Expect(3) = 5e-044
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = -2 / +1
Query: 969 KWCHLFVHFLH 937
K C LFV FLH
Sbjct: 52 KCCDLFVQFLH 84
Score = 22.1 bits (42), Expect(3) = 1e-061
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +2 / -3
Query: 821 PCNVTFH*IEIISLH 865
P +V FH*I +ISL+
Sbjct: 188 PPHVCFH*IGVISLY 144
>gb|BQ465414.1|BQ465414 HU03J08r HU Hordeum vulgare subsp. vulgare cDNA clone HU03J08
5-PRIME, mRNA sequence
Length = 685
Score = 124 bits (265), Expect(4) = 4e-044
Identities = 51/59 (86%), Positives = 54/59 (91%)
Frame = +1 / +1
Query: 142 VSSIEALHSATKALPPDFVRLDQDILSPRAGKKELYTYQTLDFWEQIKTPGMSLRCSGL 318
VSS EAL SA KA+P D+VRLDQDILSP AGKKELYTY+TLDFWEQIKTPGMSLRCSGL
Sbjct: 508 VSSFEALQSAMKAIPADYVRLDQDILSPLAGKKELYTYETLDFWEQIKTPGMSLRCSGL 684
Score = 110 bits (234), Expect(4) = 5e-036
Identities = 50/67 (74%), Positives = 55/67 (82%)
Frame = -3 / -3
Query: 314 PEHLKDIPGVLICSQKSSVWYVYSSFFPARGDKMS*SNLTKSGGSALVAE*RASMLDTKK 135
PEHLKDIPGVLICSQKSSV YV SSFFPARGDK+S*SNLT S G A +A+*RAS LDT++
Sbjct: 680 PEHLKDIPGVLICSQKSSVSYV*SSFFPARGDKIS*SNLT*SAGIAFIAD*RASKLDTRR 501
Query: 134 TNLLCLF 114
L F
Sbjct: 500 KLALSFF 480
Score = 89.0 bits (188), Expect(2) = 5e-022
Identities = 37/57 (64%), Positives = 43/57 (75%)
Frame = -2 / -2
Query: 312 RASQRHPWGFDLFPEIKCLVCVQLLLSRSRG*NVLI*PNKVWWKRLSC*MKSFNARY 142
RASQRHPW DLFP+IKCLVCV+ LLS R *++LI*PN + W L C +KSF A Y
Sbjct: 678 RASQRHPWSLDLFPKIKCLVCVKFLLSC*RR*DILI*PNIICWNCLHCGLKSFKAGY 508
Score = 85.4 bits (180), Expect(3) = 3e-023
Identities = 40/60 (66%), Positives = 46/60 (76%)
Frame = +2 / +2
Query: 140 LYLALKLFIQQLRRFHQTLLG*IKTFYPLERERRSCTHTRHLISGNRSKPQGCL*DALGY 319
+Y ALKLF Q R+F Q +LG*IK Y L +ERR+ THTRHLI GNRS+ QGCL*DAL Y
Sbjct: 506 VYPALKLFSPQ*RQFQQIMLG*IKISYLL*QERRNFTHTRHLIFGNRSRLQGCL*DALVY 685
Score = 53.8 bits (111), Expect(4) = 4e-044
Identities = 22/24 (91%), Positives = 24/24 (100%)
Frame = +2 / +1
Query: 62 YIFTPDILSAIEDVLKQKKDRANL 133
YIFTP+IL+AIEDVLKQKKDRANL
Sbjct: 430 YIFTPNILNAIEDVLKQKKDRANL 501
Score = 46.0 bits (94), Expect(4) = 5e-036
Identities = 19/25 (76%), Positives = 21/25 (84%)
Frame = -2 / -3
Query: 138 KNKFALSFFCFKTSSMALRISGVNI 64
+ K ALSFFCFKTSS+AL I GVNI
Sbjct: 506 RRKLALSFFCFKTSSIALSILGVNI 432
Score = 35.4 bits (71), Expect(2) = 5e-022
Identities = 18/35 (51%), Positives = 20/35 (57%)
Frame = -1 / -2
Query: 133 QICSVFFLF*DILNGAKDIGSKYISPQFMRSLTKS 29
QI S+F LF DIL+ K IGSKYI KS
Sbjct: 501 QISSIFLLFQDILDCIKYIGSKYIDSAIYEITHKS 397
Score = 32.7 bits (65), Expect(3) = 3e-023
Identities = 14/27 (51%), Positives = 20/27 (74%)
Frame = +1 / +3
Query: 52 IAEIYIYSRYP*RH*GCLKTEKRQSKF 132
+ +YIYS+Y * + GCL+TE+R S F
Sbjct: 420 LRSLYIYSQYT*CNRGCLETEER*S*F 500
Score = 30.4 bits (60), Expect(4) = 4e-044
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +1 / +1
Query: 1 ELLHYTEKPET 33
ELLHYTEKPET
Sbjct: 367 ELLHYTEKPET 399
Score = 28.1 bits (55), Expect(3) = 3e-023
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = +2 / +3
Query: 17 RKSRRLCE*SHKLR 58
RK+R CE*SHKLR
Sbjct: 384 RKTRDFCE*SHKLR 425
Score = 26.3 bits (51), Expect(4) = 4e-044
Identities = 11/19 (57%), Positives = 11/19 (57%)
Frame = +3 / +1
Query: 21 KAGDFVSDLINCGDIYLLP 77
K FVSDLINCG P
Sbjct: 388 KPETFVSDLINCGVYIFTP 444
Score = 26.3 bits (51), Expect(4) = 5e-036
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -1 / -3
Query: 79 IGSKYISPQFMRSLTKSPAF 20
+G +PQFMRSLTK F
Sbjct: 446 LGVNI*TPQFMRSLTKVSGF 387
Score = 24.9 bits (48), Expect(4) = 5e-036
Identities = 10/11 (90%), Positives = 11/11 (100%)
Frame = -3 / -3
Query: 35 KVSGFSV*CSS 3
KVSGFSV*C+S
Sbjct: 401 KVSGFSV*CNS 369
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 415,285,267
Number of Sequences: 312970
Number of extensions: 5894602
Number of successful extensions: 29994
Number of sequences better than 1.0e-020: 5
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 11789046920
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)