TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440503.2.1
(542 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ460471.1|BQ460471 HA09H11r HA Hordeum vulgare subsp. v... 138 9e-086
gb|BU982554.1|BU982554 HA26O10r HA Hordeum vulgare subsp. v... 108 1e-066
gb|AV925753.1|AV925753 AV925753 K. Sato unpublished cDNA li... 107 5e-065
gb|BQ657146.1|BQ657146 HA09H11u HA Hordeum vulgare subsp. v... 138 1e-059
gb|BU971682.1|BU971682 HB19F13r BC Hordeum vulgare subsp. v... 111 3e-058
gb|BF628954.2|BF628954 HVSMEb0009F03f Hordeum vulgare seedl... 103 3e-056
gb|AV930889.1|AV930889 AV930889 K. Sato unpublished cDNA li... 107 2e-055
gb|BQ760249.1|BQ760249 EBpi07_SQ003_M14_R pistil, 12 DPA, n... 108 2e-049
gb|AL511547.1|AL511547 AL511547 Hordeum vulgare Barke devel... 107 3e-047
gb|BM100425.1|BM100425 EBma01_SQ005_C23_R maternal, 4 DPA, ... 138 4e-046
gb|BQ468512.1|BQ468512 HM01H14T HM Hordeum vulgare subsp. v... 106 9e-046
gb|AL511138.1|AL511138 AL511138 Hordeum vulgare Barke devel... 138 1e-043
gb|BU988389.1|BU988389 HF17H23r HF Hordeum vulgare subsp. v... 134 5e-043
gb|BU972244.1|BU972244 HB21B09r BC Hordeum vulgare subsp. v... 107 5e-040
gb|BU972337.1|BU972337 HB21F19r BC Hordeum vulgare subsp. v... 107 5e-040
gb|BQ661228.1|BQ661228 HM02I18u HM Hordeum vulgare subsp. v... 137 5e-037
gb|BQ661003.1|BQ661003 HM01H14w HM Hordeum vulgare subsp. v... 138 2e-034
gb|BI776777.2|BI776777 EBpi07_SQ001_L15_R pistil, 12 DPA, n... 105 6e-032
gb|BM372498.2|BM372498 EBpi05_SQ003_C05_R pistil, 8 DPA, no... 101 9e-031
gb|AL512219.1|AL512219 AL512219 Hordeum vulgare Barke devel... 122 1e-027
gb|BM372501.2|BM372501 EBpi05_SQ003_C08_R pistil, 8 DPA, no... 101 1e-025
gb|BQ468849.1|BQ468849 HM02I18r HM Hordeum vulgare subsp. v... 106 1e-022
gb|BI960413.1|BI960413 HVSMEn0024I08f Hordeum vulgare rachi... 99 1e-021
gb|CA022183.1|CA022183 HZ42G07r HZ Hordeum vulgare subsp. v... 82 2e-021
gb|AV927333.1|AV927333 AV927333 K. Sato unpublished cDNA li... 51 3e-021
gb|BM372495.2|BM372495 EBpi05_SQ003_C02_R pistil, 8 DPA, no... 100 5e-021
gb|BM372479.2|BM372479 EBpi05_SQ003_B09_R pistil, 8 DPA, no... 100 5e-021
>gb|BQ460471.1|BQ460471 HA09H11r HA Hordeum vulgare subsp. vulgare cDNA clone HA09H11
5-PRIME, mRNA sequence
Length = 576
Score = 138 bits (295), Expect(3) = 9e-086
Identities = 56/66 (84%), Positives = 61/66 (92%)
Frame = +3 / +2
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
GRPASQLIGFQS+HLRA + AHVEFEVSPCKH SRA EDGRKVIDQGSHFV VG+DEFE+
Sbjct: 341 GRPASQLIGFQSVHLRADEAAHVEFEVSPCKHLSRAAEDGRKVIDQGSHFVRVGDDEFEL 520
Query: 510 SFMA*S 527
SFMA*+
Sbjct: 521 SFMA*A 538
Score = 127 bits (272), Expect(4) = 4e-067
Identities = 53/64 (82%), Positives = 54/64 (84%)
Frame = -2 / -1
Query: 520 AMKLISNSSSPTITKCEP*SITFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPISWL 341
AMKL SNSSSPT+TKCEP SIT PSS ARLKCLQGLTSNSTCA A RC LWNPISWL
Sbjct: 531 AMKLNSNSSSPTLTKCEPWSITLRPSSAARLKCLQGLTSNSTCAASSARRCTLWNPISWL 352
Query: 340 AGRP 329
AGRP
Sbjct: 351 AGRP 340
Score = 112 bits (240), Expect(2) = 3e-025
Identities = 48/61 (78%), Positives = 53/61 (86%)
Frame = -1 / -3
Query: 527 GSRHEAHLELILADHHEVRALIDHLPAILRRPAEVLAGAHLELHVRRLRRPQVQALEPDQ 348
GS HEA LEL++AD EVRAL+DHL A+LRRPA+VLAGAHLELHVR L RPQV ALEPDQ
Sbjct: 538 GSGHEAQLELVVADPDEVRALVDHLAAVLRRPAQVLAGAHLELHVRGLVRPQVHALEPDQ 359
Query: 347 L 345
L
Sbjct: 358 L 356
Score = 111 bits (237), Expect(3) = 9e-086
Identities = 41/54 (75%), Positives = 48/54 (88%)
Frame = +3 / +2
Query: 165 ATAGGMASYDVEAIGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRWPNATDG 326
A+A G SYDVE +G+E CDRL+FPAVVRVQNHGPMDG+H VL+F+RWPNATDG
Sbjct: 182 ASAAGTVSYDVEEMGAEACDRLRFPAVVRVQNHGPMDGRHPVLLFLRWPNATDG 343
Score = 108 bits (231), Expect(3) = 3e-028
Identities = 53/98 (54%), Positives = 57/98 (58%)
Frame = +2 / +1
Query: 230 QVPGGGAGAEPRAHGREALGAGVHALAQRDGRQRPAGQPADRVPEPAPEGDADGARGVRG 409
Q P G GA G A + R A QPADRVPE AP G ARGVRG
Sbjct: 241 QAPVPGGGAGAEPRAHGRQAPGAAVPAVAERDGRAARQPADRVPERAPAGGRGRARGVRG 420
Query: 410 EPLQALQPGDGGWQEGDRSGLALRDGRRG*VRDELHGV 523
EPLQAL+PG GG +GDR GLALR GRR VR ELHG+
Sbjct: 421 EPLQALEPGGGGRPQGDRPGLALRQGRRRRVRVELHGL 534
Score = 106 bits (226), Expect(3) = 9e-086
Identities = 40/62 (64%), Positives = 52/62 (83%)
Frame = +3 / +2
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFATKPPPTSNVAGLKAVEATAGGM 182
RADP+TGYPGRTYRFY+G TV+NFGYGLSYSKYSHRFA+K +++G++ ++ATA
Sbjct: 8 RADPSTGYPGRTYRFYKGKTVYNFGYGLSYSKYSHRFASKGTKPPSMSGIEGLKATARAS 187
Query: 183 AS 188
A+
Sbjct: 188 AA 193
Score = 94.1 bits (199), Expect(4) = 4e-067
Identities = 39/54 (72%), Positives = 42/54 (77%)
Frame = -2 / -1
Query: 325 PSVALGQRMNTSTECFPSMGPWFCTRTTAGNLSRSHVSDPIASTS*LAMPPAVA 164
PSVA G R N+ST C PSMGPWFCTRTTAGN S SH S PI+STS*L +P A A
Sbjct: 342 PSVAFGHRRNSSTGCLPSMGPWFCTRTTAGNRSLSHASAPISSTS*LTVPAADA 181
Score = 67.0 bits (140), Expect(4) = 4e-067
Identities = 32/38 (84%), Positives = 32/38 (84%)
Frame = -2 / -1
Query: 115 AKRWEYLE*LRP*PKLNTVGPR*NRYVRPG*PVAGSAR 2
AKRWEYLE*LRP*PKL TV P *N VRPG*PV GSAR
Sbjct: 120 AKRWEYLE*LRP*PKLYTVLPL*NL*VRPG*PVDGSAR 7
Score = 29.5 bits (58), Expect(3) = 3e-028
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1 / +3
Query: 1 CAPTRPRATPAGRTG 45
CAPTRPRAT TG
Sbjct: 6 CAPTRPRATRGAPTG 50
Score = 24.4 bits (47), Expect(3) = 3e-028
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = +3 / +2
Query: 117 TKPPPTSNVAGLKA 158
TKPP S + GLKA
Sbjct: 131 TKPPSMSGIEGLKA 172
Score = 23.1 bits (44), Expect(4) = 4e-067
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = -2 / -1
Query: 163 STALRPATLLVGGGFV 116
+ ALRP+ L+ GGFV
Sbjct: 177 AVALRPSMPLMDGGFV 130
Score = 22.6 bits (43), Expect(2) = 3e-025
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1 / -3
Query: 44 PVRPAGVARGRVGA 3
PV VARGRVGA
Sbjct: 49 PVGAPRVARGRVGA 8
>gb|BU982554.1|BU982554 HA26O10r HA Hordeum vulgare subsp. vulgare cDNA clone HA26O10
5-PRIME, mRNA sequence
Length = 609
Score = 108 bits (231), Expect(3) = 1e-066
Identities = 41/62 (66%), Positives = 51/62 (82%)
Frame = +3 / +1
Query: 141 VAGLKAVEATAGGMASYDVEAIGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRWPNAT 320
+AGL + A G++ YDVE IG+E C++LKFPAVV V+NHGPMDGKHSVL+F+RWPNAT
Sbjct: 199 LAGLTSTTAADDGVSHYDVEEIGAERCEQLKFPAVVEVENHGPMDGKHSVLLFLRWPNAT 378
Query: 321 DG 326
G
Sbjct: 379 GG 384
Score = 99.1 bits (210), Expect(3) = 1e-066
Identities = 39/62 (62%), Positives = 49/62 (79%)
Frame = +3 / +1
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
GRPASQL+GFQS HL + + A + F+VSPC+H SRA EDG+ VID+ SHF+ V EDE E+
Sbjct: 382 GRPASQLVGFQSQHLESGEKASLTFDVSPCEHLSRAREDGKMVIDRASHFLFVDEDEAEI 561
Query: 510 SF 515
SF
Sbjct: 562 SF 567
Score = 90.9 bits (192), Expect(3) = 6e-039
Identities = 39/62 (62%), Positives = 44/62 (70%)
Frame = -2 / -2
Query: 325 PSVALGQRMNTSTECFPSMGPWFCTRTTAGNLSRSHVSDPIASTS*LAMPPAVASTALRP 146
P VALG R N +TECFPSMGPWF T TTAGNLS SH S PI+STS* P + A + P
Sbjct: 383 PPVALGHRRNNNTECFPSMGPWFSTSTTAGNLSCSHRSAPISSTS*WLTPSSAAVVDVSP 204
Query: 145 AT 140
A+
Sbjct: 203 AS 198
Score = 84.0 bits (177), Expect(3) = 1e-066
Identities = 30/39 (76%), Positives = 35/39 (89%)
Frame = +3 / +1
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFAT 119
RADP TGYPGR+YRFYRGPTV+ FGYGLSYS++S R A+
Sbjct: 52 RADPVTGYPGRSYRFYRGPTVYKFGYGLSYSRFSRRLAS 168
Score = 59.7 bits (124), Expect(3) = 6e-022
Identities = 28/59 (47%), Positives = 38/59 (64%)
Frame = +2 / +3
Query: 338 GQPADRVPEPAPEGDADGARGVRGEPLQALQPGDGGWQEGDRSGLALRDGRRG*VRDEL 514
G+PA RVP+PA +G VR +P++AL+ G+GG Q+GDR L RG* RD+L
Sbjct: 390 GEPARRVPKPASRVRREG*PDVRCQPMRALEQGEGGRQDGDRQSLTFPLC*RG*GRDQL 566
Score = 57.9 bits (120), Expect(3) = 6e-022
Identities = 23/32 (71%), Positives = 26/32 (81%)
Frame = +2 / +3
Query: 227 AQVPGGGAGAEPRAHGREALGAGVHALAQRDG 322
AQVPGGG G EPR+HGR+ALG V +AQRDG
Sbjct: 285 AQVPGGGRGGEPRSHGRKALGVIVPPMAQRDG 380
Score = 56.1 bits (116), Expect(3) = 6e-039
Identities = 28/38 (73%), Positives = 29/38 (76%)
Frame = -2 / -2
Query: 115 AKRWEYLE*LRP*PKLNTVGPR*NRYVRPG*PVAGSAR 2
A R E E*LRP*P L TVGPR* R +RPG*PV GSAR
Sbjct: 164 ASRRENRE*LRP*PNL*TVGPR*KR*LRPG*PVTGSAR 51
Score = 51.5 bits (106), Expect(3) = 6e-039
Identities = 26/57 (45%), Positives = 35/57 (61%)
Frame = -1 / -1
Query: 515 EAHLELILADHHEVRALIDHLPAILRRPAEVLAGAHLELHVRRLRRPQVQALEPDQL 345
EA L LIL + E+ +DH A+L RPA+VLA A +E L ++ ALEPD+L
Sbjct: 567 EADLGLILVNKEEM*GSVDHHLAVLPRPAQVLAWADIERQASLLAGLEMLALEPDEL 397
Score = 24.9 bits (48), Expect(3) = 6e-022
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +1 / +2
Query: 1 CAPTRPRATPAGRT 42
CAPTR ATPA T
Sbjct: 50 CAPTRSPATPAVAT 91
>gb|AV925753.1|AV925753 AV925753 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd25k17 5', mRNA sequence
Length = 582
Score = 107 bits (228), Expect(3) = 5e-065
Identities = 43/67 (64%), Positives = 55/67 (82%)
Frame = +3 / +3
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
GRP SQLIGF++ HL+A + A + F+V PC+HFSRA EDG+KVID GSHF+ VG+DE E+
Sbjct: 354 GRPVSQLIGFRTQHLKAGEKASLTFDVRPCEHFSRAREDGKKVIDAGSHFLKVGKDEHEI 533
Query: 510 SFMA*SR 530
SF A*+R
Sbjct: 534 SFEA*TR 554
Score = 102 bits (217), Expect(3) = 5e-065
Identities = 40/66 (60%), Positives = 50/66 (75%)
Frame = +3 / +3
Query: 129 PTSNVAGLKAVEATAGGMASYDVEAIGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRW 308
P +N+ +A G ASY VE IG++ C++LKFPAVV VQNHGP+DGKHSVL+F+RW
Sbjct: 159 PRTNLLAGLTATPSADGGASYAVEEIGADGCEQLKFPAVVEVQNHGPLDGKHSVLMFLRW 338
Query: 309 PNATDG 326
PNAT G
Sbjct: 339 PNATGG 356
Score = 98.7 bits (209), Expect(4) = 3e-052
Identities = 45/67 (67%), Positives = 48/67 (71%)
Frame = -2 / -3
Query: 529 RDHAMKLISNSSSPTITKCEP*SITFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPI 350
R A KLIS SS PT+ KCEP SITFLPSS+A LKC GLTSN A ALRC + NPI
Sbjct: 553 RVQASKLISCSSFPTLRKCEPASITFLPSSLALLKCSHGLTSNVRLAFSPALRCWVRNPI 374
Query: 349 SWLAGRP 329
SWL GRP
Sbjct: 373 SWLTGRP 353
Score = 93.6 bits (198), Expect(4) = 3e-052
Identities = 39/53 (73%), Positives = 42/53 (79%)
Frame = -2 / -3
Query: 325 PSVALGQRMNTSTECFPSMGPWFCTRTTAGNLSRSHVSDPIASTS*LAMPPAV 167
P VALG R N STECFPS GPWFCT TTAGNLS SH S PI+ST+*LA P A+
Sbjct: 355 PPVALGHRRNISTECFPSRGPWFCTSTTAGNLSCSHPSAPISSTA*LAPPSAL 197
Score = 77.1 bits (162), Expect(3) = 3e-027
Identities = 32/67 (47%), Positives = 43/67 (64%)
Frame = +2 / +2
Query: 329 RPAGQPADRVPEPAPEGDADGARGVRGEPLQALQPGDGGWQEGDRSGLALRDGRRG*VRD 508
R +G+PAD VP PA +G +G VR + ++AL G+GGWQEGDR L GR+ RD
Sbjct: 353 RASGEPADWVPNPASQGRREGQPHVRRQAMRAL*QGEGGWQEGDRCRLTFPQGRKRRARD 532
Query: 509 ELHGVIP 529
+L G+ P
Sbjct: 533 QLRGLNP 553
Score = 76.7 bits (161), Expect(3) = 5e-065
Identities = 28/38 (73%), Positives = 33/38 (86%)
Frame = +3 / +3
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFA 116
RADPATGYPGR+YRFY+G V+ FGYGLSYSK+S + A
Sbjct: 21 RADPATGYPGRSYRFYQGNAVYKFGYGLSYSKFSRQLA 134
Score = 57.9 bits (120), Expect(3) = 3e-027
Identities = 25/33 (75%), Positives = 27/33 (81%)
Frame = +2 / +2
Query: 227 AQVPGGGAGAEPRAHGREALGAGVHALAQRDGR 325
AQVPGGG GAEPR+ GREAL A V +AQRDGR
Sbjct: 257 AQVPGGGRGAEPRSPGREALCADVPPVAQRDGR 355
Score = 46.4 bits (95), Expect(4) = 3e-052
Identities = 24/34 (70%), Positives = 25/34 (73%)
Frame = -2 / -3
Query: 103 EYLE*LRP*PKLNTVGPR*NRYVRPG*PVAGSAR 2
E LE*LRP*P L T P * R +RPG PVAGSAR
Sbjct: 121 ENLE*LRP*PNLYTALPW*KR*LRPGYPVAGSAR 20
Score = 25.4 bits (49), Expect(3) = 3e-027
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +1 / +1
Query: 1 CAPTRPRATPAGRT 42
CAPTRP T AG T
Sbjct: 19 CAPTRPPDTLAGAT 60
Score = 22.6 bits (43), Expect(4) = 3e-052
Identities = 8/21 (38%), Positives = 12/21 (57%)
Frame = -2 / -3
Query: 178 PPAVASTALRPATLLVGGGFV 116
PP+ A+RPA+ V G +
Sbjct: 211 PPSALGVAVRPASRFVRGALL 149
>gb|BQ657146.1|BQ657146 HA09H11u HA Hordeum vulgare subsp. vulgare cDNA clone HA09H11
3-PRIME, mRNA sequence
Length = 521
Score = 138 bits (295), Expect(2) = 1e-059
Identities = 56/66 (84%), Positives = 61/66 (92%)
Frame = +3 / -2
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
GRPASQLIGFQS+HLRA + AHVEFEVSPCKH SRA EDGRKVIDQGSHFV VG+DEFE+
Sbjct: 328 GRPASQLIGFQSVHLRADEAAHVEFEVSPCKHLSRAAEDGRKVIDQGSHFVRVGDDEFEL 149
Query: 510 SFMA*S 527
SFMA*+
Sbjct: 148 SFMA*A 131
Score = 127 bits (272), Expect(2) = 3e-051
Identities = 53/64 (82%), Positives = 54/64 (84%)
Frame = -2 / +3
Query: 520 AMKLISNSSSPTITKCEP*SITFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPISWL 341
AMKL SNSSSPT+TKCEP SIT PSS ARLKCLQGLTSNSTCA A RC LWNPISWL
Sbjct: 138 AMKLNSNSSSPTLTKCEPWSITLRPSSAARLKCLQGLTSNSTCAASSARRCTLWNPISWL 317
Query: 340 AGRP 329
AGRP
Sbjct: 318 AGRP 329
Score = 112 bits (240), Expect = 1e-024
Identities = 48/61 (78%), Positives = 53/61 (86%)
Frame = -1 / +2
Query: 527 GSRHEAHLELILADHHEVRALIDHLPAILRRPAEVLAGAHLELHVRRLRRPQVQALEPDQ 348
GS HEA LEL++AD EVRAL+DHL A+LRRPA+VLAGAHLELHVR L RPQV ALEPDQ
Sbjct: 131 GSGHEAQLELVVADPDEVRALVDHLAAVLRRPAQVLAGAHLELHVRGLVRPQVHALEPDQ 310
Query: 347 L 345
L
Sbjct: 311 L 313
Score = 111 bits (237), Expect(2) = 1e-059
Identities = 41/54 (75%), Positives = 48/54 (88%)
Frame = +3 / -2
Query: 165 ATAGGMASYDVEAIGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRWPNATDG 326
A+A G SYDVE +G+E CDRL+FPAVVRVQNHGPMDG+H VL+F+RWPNATDG
Sbjct: 487 ASAAGTVSYDVEEMGAEACDRLRFPAVVRVQNHGPMDGRHPVLLFLRWPNATDG 326
Score = 108 bits (231), Expect = 3e-023
Identities = 53/98 (54%), Positives = 57/98 (58%)
Frame = +2 / -1
Query: 230 QVPGGGAGAEPRAHGREALGAGVHALAQRDGRQRPAGQPADRVPEPAPEGDADGARGVRG 409
Q P G GA G A + R A QPADRVPE AP G ARGVRG
Sbjct: 428 QAPVPGGGAGAEPRAHGRQAPGAAVPAVAERDGRAARQPADRVPERAPAGGRGRARGVRG 249
Query: 410 EPLQALQPGDGGWQEGDRSGLALRDGRRG*VRDELHGV 523
EPLQAL+PG GG +GDR GLALR GRR VR ELHG+
Sbjct: 248 EPLQALEPGGGGRPQGDRPGLALRQGRRRRVRVELHGL 135
Score = 94.1 bits (199), Expect(2) = 3e-051
Identities = 39/54 (72%), Positives = 42/54 (77%)
Frame = -2 / +3
Query: 325 PSVALGQRMNTSTECFPSMGPWFCTRTTAGNLSRSHVSDPIASTS*LAMPPAVA 164
PSVA G R N+ST C PSMGPWFCTRTTAGN S SH S PI+STS*L +P A A
Sbjct: 327 PSVAFGHRRNSSTGCLPSMGPWFCTRTTAGNRSLSHASAPISSTS*LTVPAADA 488
>gb|BU971682.1|BU971682 HB19F13r BC Hordeum vulgare subsp. vulgare cDNA clone HB19F13
5-PRIME, mRNA sequence
Length = 410
Score = 111 bits (237), Expect(3) = 3e-058
Identities = 41/54 (75%), Positives = 48/54 (88%)
Frame = +3 / +3
Query: 165 ATAGGMASYDVEAIGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRWPNATDG 326
A+A G SYDVE +G+E CDRL+FPAVVRVQNHGPMDG+H VL+F+RWPNATDG
Sbjct: 123 ASAAGTVSYDVEEMGAEACDRLRFPAVVRVQNHGPMDGRHPVLLFLRWPNATDG 284
Score = 94.1 bits (199), Expect(4) = 5e-048
Identities = 39/54 (72%), Positives = 42/54 (77%)
Frame = -2 / -2
Query: 325 PSVALGQRMNTSTECFPSMGPWFCTRTTAGNLSRSHVSDPIASTS*LAMPPAVA 164
PSVA G R N+ST C PSMGPWFCTRTTAGN S SH S PI+STS*L +P A A
Sbjct: 283 PSVAFGHRRNSSTGCLPSMGPWFCTRTTAGNRSLSHASAPISSTS*LTVPAADA 122
Score = 92.7 bits (196), Expect(3) = 3e-058
Identities = 37/43 (86%), Positives = 39/43 (90%)
Frame = +3 / +3
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKV 458
GRPASQLIGFQS+HLRA + AHVEFEVSPCKH SRA EDGRKV
Sbjct: 282 GRPASQLIGFQSVHLRADEAAHVEFEVSPCKHLSRAAEDGRKV 410
Score = 88.1 bits (186), Expect(4) = 5e-048
Identities = 35/43 (81%), Positives = 35/43 (81%)
Frame = -2 / -2
Query: 457 TFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPISWLAGRP 329
T PSS ARLKCLQGLTSNSTCA A RC LWNPISWLAGRP
Sbjct: 409 TLRPSSAARLKCLQGLTSNSTCAASSARRCTLWNPISWLAGRP 281
Score = 58.3 bits (121), Expect(3) = 3e-058
Identities = 22/41 (53%), Positives = 32/41 (78%)
Frame = +3 / +3
Query: 66 FNFGYGLSYSKYSHRFATKPPPTSNVAGLKAVEATAGGMAS 188
+NFGYGLSYSKYSHRFA+K +++G++ ++ATA A+
Sbjct: 12 YNFGYGLSYSKYSHRFASKGTKPPSMSGIEGLKATARASAA 134
Score = 39.6 bits (80), Expect(4) = 5e-048
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = -2 / -2
Query: 115 AKRWEYLE*LRP*PKL 68
AKRWEYLE*LRP*PKL
Sbjct: 61 AKRWEYLE*LRP*PKL 14
Score = 23.1 bits (44), Expect(4) = 5e-048
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = -2 / -2
Query: 163 STALRPATLLVGGGFV 116
+ ALRP+ L+ GGFV
Sbjct: 118 AVALRPSMPLMDGGFV 71
>gb|BF628954.2|BF628954 HVSMEb0009F03f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0009F03f, mRNA sequence
Length = 718
Score = 103 bits (220), Expect(3) = 3e-056
Identities = 38/54 (70%), Positives = 46/54 (85%)
Frame = +3 / +2
Query: 165 ATAGGMASYDVEAIGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRWPNATDG 326
A+A G SYD+E +G+E CDRL+FPAVVRV NH PMDG+H VL+F+RWPNATDG
Sbjct: 443 ASAAGTVSYDMEDMGAEACDRLRFPAVVRVHNHRPMDGRHPVLLFLRWPNATDG 604
Score = 95.5 bits (202), Expect(3) = 3e-056
Identities = 37/62 (59%), Positives = 48/62 (77%)
Frame = +3 / +2
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFATKPPPTSNVAGLKAVEATAGGM 182
RADP+TGYPGRTYRFY+G TV+NF YGLSY KYSHR A+K ++ G++ ++ATA
Sbjct: 269 RADPSTGYPGRTYRFYKGKTVYNFCYGLSYYKYSHRLASKGTKPPSMNGIEGLKATARAS 448
Query: 183 AS 188
A+
Sbjct: 449 AA 454
Score = 84.0 bits (177), Expect(3) = 9e-042
Identities = 36/54 (66%), Positives = 39/54 (72%)
Frame = -2 / -2
Query: 325 PSVALGQRMNTSTECFPSMGPWFCTRTTAGNLSRSHVSDPIASTS*LAMPPAVA 164
PSVA G R N+ST C PSMG W CTRTTAGN S SH S PI+S S*L +P A A
Sbjct: 603 PSVAFGHRRNSSTGCLPSMGLWLCTRTTAGNRSLSHASAPISSMS*LTVPAADA 442
Score = 64.8 bits (135), Expect(3) = 9e-042
Identities = 27/39 (69%), Positives = 28/39 (71%)
Frame = -2 / -2
Query: 445 SSVARLKCLQGLTSNSTCAVCVALRCRLWNPISWLAGRP 329
SS ARLK Q LT NSTCA A RC LWNPISW+A RP
Sbjct: 717 SSAARLKGFQRLT*NSTCAALSARRCTLWNPISWMADRP 601
Score = 59.3 bits (123), Expect(3) = 9e-042
Identities = 30/38 (78%), Positives = 30/38 (78%)
Frame = -2 / -2
Query: 115 AKRWEYLE*LRP*PKLNTVGPR*NRYVRPG*PVAGSAR 2
AKRWEYL *LRP* KL TV P *N VRPG*PV GSAR
Sbjct: 381 AKRWEYL**LRP*QKLYTVLPL*NL*VRPG*PVDGSAR 268
Score = 57.9 bits (120), Expect(3) = 3e-056
Identities = 27/39 (69%), Positives = 28/39 (71%)
Frame = +3 / +2
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATED 446
GR A QLIGFQS+HLRA AHVEF VS K SRA ED
Sbjct: 602 GRSAIQLIGFQSVHLRADNAAHVEF*VSLWKPLSRAAED 718
Score = 54.7 bits (113), Expect(4) = 4e-022
Identities = 24/39 (61%), Positives = 26/39 (66%)
Frame = +2 / +1
Query: 329 RPAGQPADRVPEPAPEGDADGARGVRGEPLQALQPGDGG 445
R PADRVPE AP G ARGV GEPL+AL+PG G
Sbjct: 601 RAVRHPADRVPERAPAGGQCRARGVLGEPLEALEPGRRG 717
Score = 51.5 bits (106), Expect(4) = 4e-022
Identities = 23/33 (69%), Positives = 26/33 (78%)
Frame = +2 / +1
Query: 227 AQVPGGGAGAEPRAHGREALGAGVHALAQRDGR 325
A VP GGAGA P+AHGR A GA V A+A+RDGR
Sbjct: 505 APVPCGGAGA*PQAHGR*APGAAVPAVAERDGR 603
Score = 29.5 bits (58), Expect(4) = 4e-022
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1 / +3
Query: 1 CAPTRPRATPAGRTG 45
CAPTRPRAT TG
Sbjct: 267 CAPTRPRATRGAPTG 311
Score = 23.1 bits (44), Expect(4) = 4e-022
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +3 / +2
Query: 117 TKPPPTSNVAGLKA 158
TKPP + + GLKA
Sbjct: 392 TKPPSMNGIEGLKA 433
>gb|AV930889.1|AV930889 AV930889 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd25k17 3', mRNA sequence
Length = 673
Score = 107 bits (228), Expect(3) = 2e-055
Identities = 43/67 (64%), Positives = 55/67 (82%)
Frame = +3 / -3
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
GRP SQLIGF++ HL+A + A + F+V PC+HFSRA EDG+KVID GSHF+ VG+DE E+
Sbjct: 371 GRPVSQLIGFRTQHLKAGEKASLTFDVRPCEHFSRAREDGKKVIDAGSHFLKVGKDEHEI 192
Query: 510 SFMA*SR 530
SF A*+R
Sbjct: 191 SFEA*TR 171
Score = 100 bits (213), Expect(3) = 2e-055
Identities = 39/66 (59%), Positives = 49/66 (74%)
Frame = +3 / -3
Query: 129 PTSNVAGLKAVEATAGGMASYDVEAIGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRW 308
P +N+ +A G A Y VE IG++ C++LKFPAVV VQNHGP+DGKHSVL+F+RW
Sbjct: 566 PRTNLLAGLTATPSADGGAXYAVEEIGADGCEQLKFPAVVEVQNHGPLDGKHSVLMFLRW 387
Query: 309 PNATDG 326
PNAT G
Sbjct: 386 PNATGG 369
Score = 98.7 bits (209), Expect(4) = 6e-044
Identities = 45/67 (67%), Positives = 48/67 (71%)
Frame = -2 / +1
Query: 529 RDHAMKLISNSSSPTITKCEP*SITFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPI 350
R A KLIS SS PT+ KCEP SITFLPSS+A LKC GLTSN A ALRC + NPI
Sbjct: 172 RVQASKLISCSSFPTLRKCEPASITFLPSSLALLKCSHGLTSNVRLAFSPALRCWVRNPI 351
Query: 349 SWLAGRP 329
SWL GRP
Sbjct: 352 SWLTGRP 372
Score = 91.3 bits (193), Expect(4) = 6e-044
Identities = 38/53 (71%), Positives = 41/53 (77%)
Frame = -2 / +1
Query: 325 PSVALGQRMNTSTECFPSMGPWFCTRTTAGNLSRSHVSDPIASTS*LAMPPAV 167
P VALG R N STECFPS GPWFCT TTAGNLS SH S PI+ST+ LA P A+
Sbjct: 370 PPVALGHRRNISTECFPSRGPWFCTSTTAGNLSCSHPSAPISSTAXLAPPSAL 528
Score = 77.1 bits (162), Expect(2) = 3e-025
Identities = 32/67 (47%), Positives = 43/67 (64%)
Frame = +2 / -2
Query: 329 RPAGQPADRVPEPAPEGDADGARGVRGEPLQALQPGDGGWQEGDRSGLALRDGRRG*VRD 508
R +G+PAD VP PA +G +G VR + ++AL G+GGWQEGDR L GR+ RD
Sbjct: 372 RASGEPADWVPNPASQGRREGQPHVRRQAMRAL*QGEGGWQEGDRCRLTFPQGRKRRARD 193
Query: 509 ELHGVIP 529
+L G+ P
Sbjct: 192 QLRGLNP 172
Score = 57.9 bits (120), Expect(2) = 3e-025
Identities = 25/33 (75%), Positives = 27/33 (81%)
Frame = +2 / -2
Query: 227 AQVPGGGAGAEPRAHGREALGAGVHALAQRDGR 325
AQVPGGG GAEPR+ GREAL A V +AQRDGR
Sbjct: 468 AQVPGGGRGAEPRSPGREALCADVPPVAQRDGR 370
Score = 46.4 bits (95), Expect(3) = 2e-055
Identities = 16/27 (59%), Positives = 21/27 (77%)
Frame = +3 / -3
Query: 36 TYRFYRGPTVFNFGYGLSYSKYSHRFA 116
+YRFY+G V+ FGYG SYSK+S + A
Sbjct: 671 SYRFYQGNXVYKFGYGFSYSKFSRQLA 591
Score = 22.6 bits (43), Expect(4) = 6e-044
Identities = 8/21 (38%), Positives = 12/21 (57%)
Frame = -2 / +1
Query: 178 PPAVASTALRPATLLVGGGFV 116
PP+ A+RPA+ V G +
Sbjct: 514 PPSALGVAVRPASRFVRGALL 576
Score = 21.7 bits (41), Expect(4) = 6e-044
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = -2 / +1
Query: 103 EYLE*LRP*PKLNTVGPR*NR 41
E LE*L+P P L T P * R
Sbjct: 604 ENLE*LKPXPNLYTXLPW*KR 666
>gb|BQ760249.1|BQ760249 EBpi07_SQ003_M14_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ003_M14 5', mRNA sequence
Length = 518
Score = 108 bits (231), Expect(2) = 2e-049
Identities = 40/54 (74%), Positives = 47/54 (87%)
Frame = +3 / +1
Query: 165 ATAGGMASYDVEAIGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRWPNATDG 326
A+A G SYDVE +G+E CDRL+FPAVVRV NHGPMDG+H VL+F+RWPNATDG
Sbjct: 355 ASAAGTVSYDVEEMGAEACDRLRFPAVVRVXNHGPMDGRHPVLLFLRWPNATDG 516
Score = 106 bits (226), Expect(2) = 2e-049
Identities = 40/62 (64%), Positives = 52/62 (83%)
Frame = +3 / +1
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFATKPPPTSNVAGLKAVEATAGGM 182
RADP+TGYPGRTYRFY+G TV+NFGYGLSYSKYSHRFA+K +++G++ ++ATA
Sbjct: 181 RADPSTGYPGRTYRFYKGKTVYNFGYGLSYSKYSHRFASKGTKPPSMSGIEGLKATARAS 360
Query: 183 AS 188
A+
Sbjct: 361 AA 366
Score = 90.9 bits (192), Expect(3) = 9e-034
Identities = 38/54 (70%), Positives = 41/54 (75%)
Frame = -2 / -1
Query: 325 PSVALGQRMNTSTECFPSMGPWFCTRTTAGNLSRSHVSDPIASTS*LAMPPAVA 164
PSVA G R N+ST C PSMGPW CTRTTAGN S SH S PI+STS*L +P A A
Sbjct: 515 PSVAFGHRRNSSTGCLPSMGPWXCTRTTAGNRSLSHASAPISSTS*LTVPAADA 354
Score = 67.0 bits (140), Expect(3) = 9e-034
Identities = 32/38 (84%), Positives = 32/38 (84%)
Frame = -2 / -1
Query: 115 AKRWEYLE*LRP*PKLNTVGPR*NRYVRPG*PVAGSAR 2
AKRWEYLE*LRP*PKL TV P *N VRPG*PV GSAR
Sbjct: 293 AKRWEYLE*LRP*PKLYTVLPL*NL*VRPG*PVDGSAR 180
Score = 23.1 bits (44), Expect(3) = 9e-034
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = -2 / -1
Query: 163 STALRPATLLVGGGFV 116
+ ALRP+ L+ GGFV
Sbjct: 350 AVALRPSMPLMDGGFV 303
>gb|AL511547.1|AL511547 AL511547 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY08G20u 3',
mRNA sequence
Length = 566
Score = 107 bits (228), Expect(2) = 3e-047
Identities = 43/67 (64%), Positives = 55/67 (82%)
Frame = +3 / -1
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
GRP SQLIGF++ HL+A + A + F+V PC+HFSRA EDG+KVID GSHF+ VG+DE E+
Sbjct: 314 GRPVSQLIGFRTQHLKAGEKASLTFDVRPCEHFSRAREDGKKVIDAGSHFLKVGKDEHEI 135
Query: 510 SFMA*SR 530
SF A*+R
Sbjct: 134 SFEA*TR 114
Score = 100 bits (214), Expect(2) = 3e-047
Identities = 39/66 (59%), Positives = 50/66 (75%)
Frame = +3 / -1
Query: 129 PTSNVAGLKAVEATAGGMASYDVEAIGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRW 308
P++N+ +A G A Y VE IG++ C++LKFPAVV VQNHGP+DGKHSVL+F+RW
Sbjct: 509 PSTNLLAGLTATPSADGGARYAVEEIGADGCEQLKFPAVVEVQNHGPLDGKHSVLMFLRW 330
Query: 309 PNATDG 326
PNAT G
Sbjct: 329 PNATGG 312
Score = 98.7 bits (209), Expect(2) = 6e-042
Identities = 45/67 (67%), Positives = 48/67 (71%)
Frame = -2 / +1
Query: 529 RDHAMKLISNSSSPTITKCEP*SITFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPI 350
R A KLIS SS PT+ KCEP SITFLPSS+A LKC GLTSN A ALRC + NPI
Sbjct: 115 RVQASKLISCSSFPTLRKCEPASITFLPSSLALLKCSHGLTSNVRLAFSPALRCWVRNPI 294
Query: 349 SWLAGRP 329
SWL GRP
Sbjct: 295 SWLTGRP 315
Score = 91.8 bits (194), Expect(2) = 6e-042
Identities = 39/66 (59%), Positives = 45/66 (68%)
Frame = -2 / +1
Query: 325 PSVALGQRMNTSTECFPSMGPWFCTRTTAGNLSRSHVSDPIASTS*LAMPPAVASTALRP 146
P VALG R N STECFPS GPWFCT TTAGNLS SH S PI+ST+ LA P A+
Sbjct: 313 PPVALGHRRNISTECFPSRGPWFCTSTTAGNLSCSHPSAPISSTAYLAPPSALGVAVRPA 492
Query: 145 ATLLVG 128
+ ++G
Sbjct: 493 SRFVLG 510
Score = 77.1 bits (162), Expect(2) = 3e-025
Identities = 32/67 (47%), Positives = 43/67 (64%)
Frame = +2 / -3
Query: 329 RPAGQPADRVPEPAPEGDADGARGVRGEPLQALQPGDGGWQEGDRSGLALRDGRRG*VRD 508
R +G+PAD VP PA +G +G VR + ++AL G+GGWQEGDR L GR+ RD
Sbjct: 315 RASGEPADWVPNPASQGRREGQPHVRRQAMRAL*QGEGGWQEGDRCRLTFPQGRKRRARD 136
Query: 509 ELHGVIP 529
+L G+ P
Sbjct: 135 QLRGLNP 115
Score = 57.9 bits (120), Expect(2) = 3e-025
Identities = 25/33 (75%), Positives = 27/33 (81%)
Frame = +2 / -3
Query: 227 AQVPGGGAGAEPRAHGREALGAGVHALAQRDGR 325
AQVPGGG GAEPR+ GREAL A V +AQRDGR
Sbjct: 411 AQVPGGGRGAEPRSPGREALCADVPPVAQRDGR 313
>gb|BM100425.1|BM100425 EBma01_SQ005_C23_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ005_C23 5', mRNA sequence
Length = 426
Score = 138 bits (295), Expect(2) = 4e-046
Identities = 56/66 (84%), Positives = 61/66 (92%)
Frame = +3 / +3
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
GRPASQLIGFQS+HLRA + AHVEFEVSPCKH SRA EDGRKVIDQGSHFV VG+DEFE+
Sbjct: 81 GRPASQLIGFQSVHLRADEAAHVEFEVSPCKHLSRAAEDGRKVIDQGSHFVRVGDDEFEL 260
Query: 510 SFMA*S 527
SFMA*+
Sbjct: 261 SFMA*A 278
Score = 127 bits (272), Expect(2) = 8e-041
Identities = 53/64 (82%), Positives = 54/64 (84%)
Frame = -2 / -3
Query: 520 AMKLISNSSSPTITKCEP*SITFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPISWL 341
AMKL SNSSSPT+TKCEP SIT PSS ARLKCLQGLTSNSTCA A RC LWNPISWL
Sbjct: 271 AMKLNSNSSSPTLTKCEPWSITLRPSSAARLKCLQGLTSNSTCAASSARRCTLWNPISWL 92
Query: 340 AGRP 329
AGRP
Sbjct: 91 AGRP 80
Score = 112 bits (240), Expect = 1e-024
Identities = 48/61 (78%), Positives = 53/61 (86%)
Frame = -1 / -2
Query: 527 GSRHEAHLELILADHHEVRALIDHLPAILRRPAEVLAGAHLELHVRRLRRPQVQALEPDQ 348
GS HEA LEL++AD EVRAL+DHL A+LRRPA+VLAGAHLELHVR L RPQV ALEPDQ
Sbjct: 278 GSGHEAQLELVVADPDEVRALVDHLAAVLRRPAQVLAGAHLELHVRGLVRPQVHALEPDQ 99
Query: 347 L 345
L
Sbjct: 98 L 96
Score = 103 bits (220), Expect(2) = 1e-031
Identities = 46/65 (70%), Positives = 49/65 (75%)
Frame = +2 / +2
Query: 329 RPAGQPADRVPEPAPEGDADGARGVRGEPLQALQPGDGGWQEGDRSGLALRDGRRG*VRD 508
R A QPADRVPE AP G ARGVRGEPLQAL+PG GG +GDR GLALR GRR VR
Sbjct: 80 RAARQPADRVPERAPAGGRGRARGVRGEPLQALEPGGGGRPQGDRPGLALRQGRRRRVRV 259
Query: 509 ELHGV 523
ELHG+
Sbjct: 260 ELHGL 274
Score = 66.1 bits (138), Expect(2) = 4e-046
Identities = 23/27 (85%), Positives = 26/27 (96%)
Frame = +3 / +3
Query: 246 VRVQNHGPMDGKHSVLVFMRWPNATDG 326
VRVQNHGPMDG+H VL+F+RWPNATDG
Sbjct: 3 VRVQNHGPMDGRHPVLLFLRWPNATDG 83
Score = 58.8 bits (122), Expect(2) = 8e-041
Identities = 21/27 (77%), Positives = 22/27 (81%)
Frame = -2 / -3
Query: 325 PSVALGQRMNTSTECFPSMGPWFCTRT 245
PSVA G R N+ST C PSMGPWFCTRT
Sbjct: 82 PSVAFGHRRNSSTGCLPSMGPWFCTRT 2
Score = 52.4 bits (108), Expect(2) = 1e-031
Identities = 21/27 (77%), Positives = 24/27 (88%)
Frame = +2 / +2
Query: 245 GAGAEPRAHGREALGAGVHALAQRDGR 325
GAGAEPRAHGR+A GA V A+A+RDGR
Sbjct: 2 GAGAEPRAHGRQAPGAAVPAVAERDGR 82
>gb|BQ468512.1|BQ468512 HM01H14T HM Hordeum vulgare subsp. vulgare cDNA clone HM01H14
5-PRIME, mRNA sequence
Length = 600
Score = 106 bits (226), Expect(2) = 9e-046
Identities = 40/62 (64%), Positives = 52/62 (83%)
Frame = +3 / +1
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFATKPPPTSNVAGLKAVEATAGGM 182
RADP+TGYPGRTYRFY+G TV+NFGYGLSYSKYSHRFA+K +++G++ ++ATA
Sbjct: 280 RADPSTGYPGRTYRFYKGKTVYNFGYGLSYSKYSHRFASKGTKPPSMSGIEGLKATARAS 459
Query: 183 AS 188
A+
Sbjct: 460 AA 465
Score = 96.8 bits (205), Expect(2) = 9e-046
Identities = 35/49 (71%), Positives = 42/49 (85%)
Frame = +3 / +1
Query: 165 ATAGGMASYDVEAIGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRWP 311
A+A G SYDVE +G+E CDRL+FPAVVRV NHGPMDG+H VL+F+RWP
Sbjct: 454 ASAAGTVSYDVEEMGAEACDRLRFPAVVRVHNHGPMDGRHPVLLFLRWP 600
Score = 82.6 bits (174), Expect(3) = 2e-031
Identities = 34/49 (69%), Positives = 37/49 (75%)
Frame = -2 / -2
Query: 310 GQRMNTSTECFPSMGPWFCTRTTAGNLSRSHVSDPIASTS*LAMPPAVA 164
G R N+ST C PSMGPW CTRTTAGN S SH S PI+STS*L +P A A
Sbjct: 599 GHRRNSSTGCLPSMGPWLCTRTTAGNRSLSHASAPISSTS*LTVPAADA 453
Score = 67.0 bits (140), Expect(3) = 2e-031
Identities = 32/38 (84%), Positives = 32/38 (84%)
Frame = -2 / -2
Query: 115 AKRWEYLE*LRP*PKLNTVGPR*NRYVRPG*PVAGSAR 2
AKRWEYLE*LRP*PKL TV P *N VRPG*PV GSAR
Sbjct: 392 AKRWEYLE*LRP*PKLYTVLPL*NL*VRPG*PVDGSAR 279
Score = 23.1 bits (44), Expect(3) = 2e-031
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = -2 / -2
Query: 163 STALRPATLLVGGGFV 116
+ ALRP+ L+ GGFV
Sbjct: 449 AVALRPSMPLMDGGFV 402
>gb|AL511138.1|AL511138 AL511138 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY07B24u 3',
mRNA sequence
Length = 440
Score = 138 bits (295), Expect(2) = 1e-043
Identities = 56/66 (84%), Positives = 61/66 (92%)
Frame = +3 / -1
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
GRPASQLIGFQS+HLRA + AHVEFEVSPCKH SRA EDGRKVIDQGSHFV VG+DEFE+
Sbjct: 374 GRPASQLIGFQSVHLRADEAAHVEFEVSPCKHLSRAAEDGRKVIDQGSHFVRVGDDEFEL 195
Query: 510 SFMA*S 527
SFMA*+
Sbjct: 194 SFMA*A 177
Score = 127 bits (272), Expect(2) = 1e-036
Identities = 53/64 (82%), Positives = 54/64 (84%)
Frame = -2 / +1
Query: 520 AMKLISNSSSPTITKCEP*SITFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPISWL 341
AMKL SNSSSPT+TKCEP SIT PSS ARLKCLQGLTSNSTCA A RC LWNPISWL
Sbjct: 184 AMKLNSNSSSPTLTKCEPWSITLRPSSAARLKCLQGLTSNSTCAASSARRCTLWNPISWL 363
Query: 340 AGRP 329
AGRP
Sbjct: 364 AGRP 375
Score = 112 bits (240), Expect = 1e-024
Identities = 48/61 (78%), Positives = 53/61 (86%)
Frame = -1 / +3
Query: 527 GSRHEAHLELILADHHEVRALIDHLPAILRRPAEVLAGAHLELHVRRLRRPQVQALEPDQ 348
GS HEA LEL++AD EVRAL+DHL A+LRRPA+VLAGAHLELHVR L RPQV ALEPDQ
Sbjct: 177 GSGHEAQLELVVADPDEVRALVDHLAAVLRRPAQVLAGAHLELHVRGLVRPQVHALEPDQ 356
Query: 347 L 345
L
Sbjct: 357 L 359
Score = 103 bits (220), Expect(2) = 4e-028
Identities = 46/65 (70%), Positives = 49/65 (75%)
Frame = +2 / -3
Query: 329 RPAGQPADRVPEPAPEGDADGARGVRGEPLQALQPGDGGWQEGDRSGLALRDGRRG*VRD 508
R A QPADRVPE AP G ARGVRGEPLQAL+PG GG +GDR GLALR GRR VR
Sbjct: 375 RAARQPADRVPERAPAGGRGRARGVRGEPLQALEPGGGGRPQGDRPGLALRQGRRRRVRV 196
Query: 509 ELHGV 523
ELHG+
Sbjct: 195 ELHGL 181
Score = 57.9 bits (120), Expect(2) = 1e-043
Identities = 19/23 (82%), Positives = 22/23 (95%)
Frame = +3 / -1
Query: 258 NHGPMDGKHSVLVFMRWPNATDG 326
NHGPMDG+H VL+F+RWPNATDG
Sbjct: 440 NHGPMDGRHPVLLFLRWPNATDG 372
Score = 45.1 bits (92), Expect(2) = 1e-036
Identities = 16/22 (72%), Positives = 17/22 (77%)
Frame = -2 / +1
Query: 325 PSVALGQRMNTSTECFPSMGPW 260
PSVA G R N+ST C PSMGPW
Sbjct: 373 PSVAFGHRRNSSTGCLPSMGPW 438
Score = 40.9 bits (83), Expect(2) = 4e-028
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = +2 / -3
Query: 260 PRAHGREALGAGVHALAQRDGR 325
PRAHGR+A GA V A+A+RDGR
Sbjct: 438 PRAHGRQAPGAAVPAVAERDGR 373
>gb|BU988389.1|BU988389 HF17H23r HF Hordeum vulgare subsp. vulgare cDNA clone HF17H23
5-PRIME, mRNA sequence
Length = 426
Score = 134 bits (287), Expect(2) = 5e-043
Identities = 55/66 (83%), Positives = 59/66 (89%)
Frame = +3 / +1
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
GRPASQLIGFQS+HLRA HVEFEVSPCKH SRA EDGRKVIDQGSHFV VG+DEFE+
Sbjct: 82 GRPASQLIGFQSVHLRADDVFHVEFEVSPCKHLSRAAEDGRKVIDQGSHFVRVGDDEFEL 261
Query: 510 SFMA*S 527
SFMA*+
Sbjct: 262 SFMA*A 279
Score = 119 bits (254), Expect(2) = 5e-036
Identities = 51/64 (79%), Positives = 52/64 (81%)
Frame = -2 / -2
Query: 520 AMKLISNSSSPTITKCEP*SITFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPISWL 341
AMKL SNSSSPT+TKCEP SIT PSS ARLKCLQGLTSNST A RC LWNPISWL
Sbjct: 272 AMKLNSNSSSPTLTKCEPWSITLRPSSAARLKCLQGLTSNST*KTSSARRCTLWNPISWL 93
Query: 340 AGRP 329
AGRP
Sbjct: 92 AGRP 81
Score = 110 bits (235), Expect = 6e-024
Identities = 46/61 (75%), Positives = 53/61 (86%)
Frame = -1 / -1
Query: 527 GSRHEAHLELILADHHEVRALIDHLPAILRRPAEVLAGAHLELHVRRLRRPQVQALEPDQ 348
GS HEA LEL++AD EVRAL+DHL A+LRRPA+VLAGAHLELHV+ + RPQV ALEPDQ
Sbjct: 279 GSGHEAQLELVVADPDEVRALVDHLAAVLRRPAQVLAGAHLELHVKDIVRPQVHALEPDQ 100
Query: 347 L 345
L
Sbjct: 99 L 97
Score = 100 bits (213), Expect(2) = 6e-029
Identities = 45/65 (69%), Positives = 49/65 (75%)
Frame = +2 / +3
Query: 329 RPAGQPADRVPEPAPEGDADGARGVRGEPLQALQPGDGGWQEGDRSGLALRDGRRG*VRD 508
R A QPADRVPE AP G +RGVRGEPLQAL+PG GG +GDR GLALR GRR VR
Sbjct: 81 RAARQPADRVPERAPAGGRCLSRGVRGEPLQALEPGGGGRPQGDRPGLALRQGRRRRVRV 260
Query: 509 ELHGV 523
ELHG+
Sbjct: 261 ELHGL 275
Score = 59.3 bits (123), Expect(2) = 5e-043
Identities = 20/25 (80%), Positives = 23/25 (92%)
Frame = +3 / +1
Query: 252 VQNHGPMDGKHSVLVFMRWPNATDG 326
VQ HGPMDG+H VL+F+RWPNATDG
Sbjct: 10 VQTHGPMDGRHPVLLFLRWPNATDG 84
Score = 51.0 bits (105), Expect(2) = 5e-036
Identities = 18/25 (72%), Positives = 19/25 (76%)
Frame = -2 / -2
Query: 325 PSVALGQRMNTSTECFPSMGPWFCT 251
PSVA G R N+ST C PSMGPW CT
Sbjct: 83 PSVAFGHRRNSSTGCLPSMGPWVCT 9
Score = 46.4 bits (95), Expect(2) = 6e-029
Identities = 18/25 (72%), Positives = 22/25 (88%)
Frame = +2 / +3
Query: 251 GAEPRAHGREALGAGVHALAQRDGR 325
GA+PRAHGR+A GA V A+A+RDGR
Sbjct: 9 GADPRAHGRQAPGAAVPAVAERDGR 83
>gb|BU972244.1|BU972244 HB21B09r BC Hordeum vulgare subsp. vulgare cDNA clone HB21B09
5-PRIME, mRNA sequence
Length = 513
Score = 107 bits (228), Expect(2) = 5e-040
Identities = 43/67 (64%), Positives = 55/67 (82%)
Frame = +3 / +3
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
GRP SQLIGF++ HL+A + A + F+V PC+HFSRA EDG+KVID GSHF+ VG+DE E+
Sbjct: 108 GRPVSQLIGFRTQHLKAGEKASLTFDVRPCEHFSRAREDGKKVIDAGSHFLKVGKDEHEI 287
Query: 510 SFMA*SR 530
SF A*+R
Sbjct: 288 SFEA*TR 308
Score = 98.7 bits (209), Expect(2) = 8e-036
Identities = 45/67 (67%), Positives = 48/67 (71%)
Frame = -2 / -3
Query: 529 RDHAMKLISNSSSPTITKCEP*SITFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPI 350
R A KLIS SS PT+ KCEP SITFLPSS+A LKC GLTSN A ALRC + NPI
Sbjct: 307 RVQASKLISCSSFPTLRKCEPASITFLPSSLALLKCSHGLTSNVRLAFSPALRCWVRNPI 128
Query: 349 SWLAGRP 329
SWL GRP
Sbjct: 127 SWLTGRP 107
Score = 77.1 bits (162), Expect(2) = 3e-025
Identities = 32/67 (47%), Positives = 43/67 (64%)
Frame = +2 / +2
Query: 329 RPAGQPADRVPEPAPEGDADGARGVRGEPLQALQPGDGGWQEGDRSGLALRDGRRG*VRD 508
R +G+PAD VP PA +G +G VR + ++AL G+GGWQEGDR L GR+ RD
Sbjct: 107 RASGEPADWVPNPASQGRREGQPHVRRQAMRAL*QGEGGWQEGDRCRLTFPQGRKRRARD 286
Query: 509 ELHGVIP 529
+L G+ P
Sbjct: 287 QLRGLNP 307
Score = 76.7 bits (161), Expect(2) = 5e-040
Identities = 28/33 (84%), Positives = 31/33 (93%)
Frame = +3 / +3
Query: 228 LKFPAVVRVQNHGPMDGKHSVLVFMRWPNATDG 326
LKFPAVV VQNHGP+DGKHSVL+F+RWPNAT G
Sbjct: 12 LKFPAVVEVQNHGPLDGKHSVLMFLRWPNATGG 110
Score = 71.2 bits (149), Expect(2) = 8e-036
Identities = 27/33 (81%), Positives = 27/33 (81%)
Frame = -2 / -3
Query: 325 PSVALGQRMNTSTECFPSMGPWFCTRTTAGNLS 227
P VALG R N STECFPS GPWFCT TTAGNLS
Sbjct: 109 PPVALGHRRNISTECFPSRGPWFCTSTTAGNLS 11
Score = 57.9 bits (120), Expect(2) = 3e-025
Identities = 25/33 (75%), Positives = 27/33 (81%)
Frame = +2 / +2
Query: 227 AQVPGGGAGAEPRAHGREALGAGVHALAQRDGR 325
AQVPGGG GAEPR+ GREAL A V +AQRDGR
Sbjct: 11 AQVPGGGRGAEPRSPGREALCADVPPVAQRDGR 109
>gb|BU972337.1|BU972337 HB21F19r BC Hordeum vulgare subsp. vulgare cDNA clone HB21F19
5-PRIME, mRNA sequence
Length = 513
Score = 107 bits (228), Expect(2) = 5e-040
Identities = 43/67 (64%), Positives = 55/67 (82%)
Frame = +3 / +3
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
GRP SQLIGF++ HL+A + A + F+V PC+HFSRA EDG+KVID GSHF+ VG+DE E+
Sbjct: 108 GRPVSQLIGFRTQHLKAGEKASLTFDVRPCEHFSRAREDGKKVIDAGSHFLKVGKDEHEI 287
Query: 510 SFMA*SR 530
SF A*+R
Sbjct: 288 SFEA*TR 308
Score = 98.7 bits (209), Expect(2) = 8e-036
Identities = 45/67 (67%), Positives = 48/67 (71%)
Frame = -2 / -3
Query: 529 RDHAMKLISNSSSPTITKCEP*SITFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPI 350
R A KLIS SS PT+ KCEP SITFLPSS+A LKC GLTSN A ALRC + NPI
Sbjct: 307 RVQASKLISCSSFPTLRKCEPASITFLPSSLALLKCSHGLTSNVRLAFSPALRCWVRNPI 128
Query: 349 SWLAGRP 329
SWL GRP
Sbjct: 127 SWLTGRP 107
Score = 77.1 bits (162), Expect(2) = 3e-025
Identities = 32/67 (47%), Positives = 43/67 (64%)
Frame = +2 / +2
Query: 329 RPAGQPADRVPEPAPEGDADGARGVRGEPLQALQPGDGGWQEGDRSGLALRDGRRG*VRD 508
R +G+PAD VP PA +G +G VR + ++AL G+GGWQEGDR L GR+ RD
Sbjct: 107 RASGEPADWVPNPASQGRREGQPHVRRQAMRAL*QGEGGWQEGDRCRLTFPQGRKRRARD 286
Query: 509 ELHGVIP 529
+L G+ P
Sbjct: 287 QLRGLNP 307
Score = 76.7 bits (161), Expect(2) = 5e-040
Identities = 28/33 (84%), Positives = 31/33 (93%)
Frame = +3 / +3
Query: 228 LKFPAVVRVQNHGPMDGKHSVLVFMRWPNATDG 326
LKFPAVV VQNHGP+DGKHSVL+F+RWPNAT G
Sbjct: 12 LKFPAVVEVQNHGPLDGKHSVLMFLRWPNATGG 110
Score = 71.2 bits (149), Expect(2) = 8e-036
Identities = 27/33 (81%), Positives = 27/33 (81%)
Frame = -2 / -3
Query: 325 PSVALGQRMNTSTECFPSMGPWFCTRTTAGNLS 227
P VALG R N STECFPS GPWFCT TTAGNLS
Sbjct: 109 PPVALGHRRNISTECFPSRGPWFCTSTTAGNLS 11
Score = 57.9 bits (120), Expect(2) = 3e-025
Identities = 25/33 (75%), Positives = 27/33 (81%)
Frame = +2 / +2
Query: 227 AQVPGGGAGAEPRAHGREALGAGVHALAQRDGR 325
AQVPGGG GAEPR+ GREAL A V +AQRDGR
Sbjct: 11 AQVPGGGRGAEPRSPGREALCADVPPVAQRDGR 109
>gb|BQ661228.1|BQ661228 HM02I18u HM Hordeum vulgare subsp. vulgare cDNA clone HM02I18
3-PRIME, mRNA sequence
Length = 385
Score = 137 bits (294), Expect(2) = 5e-037
Identities = 56/66 (84%), Positives = 61/66 (92%)
Frame = +3 / -1
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
GRPASQLIGFQS+HLRA + AHVEFEVSPCKH SRA EDGRKVIDQGSHFV VG+DEFE+
Sbjct: 340 GRPASQLIGFQSVHLRADKAAHVEFEVSPCKHLSRAAEDGRKVIDQGSHFVRVGDDEFEL 161
Query: 510 SFMA*S 527
SFMA*+
Sbjct: 160 SFMA*A 143
Score = 127 bits (272), Expect = 5e-029
Identities = 53/64 (82%), Positives = 54/64 (84%)
Frame = -2 / +3
Query: 520 AMKLISNSSSPTITKCEP*SITFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPISWL 341
AMKL SNSSSPT+TKCEP SIT PSS ARLKCLQGLTSNSTCA A RC LWNPISWL
Sbjct: 150 AMKLNSNSSSPTLTKCEPWSITLRPSSAARLKCLQGLTSNSTCAALSARRCTLWNPISWL 329
Query: 340 AGRP 329
AGRP
Sbjct: 330 AGRP 341
Score = 112 bits (240), Expect = 1e-024
Identities = 48/61 (78%), Positives = 53/61 (86%)
Frame = -1 / +2
Query: 527 GSRHEAHLELILADHHEVRALIDHLPAILRRPAEVLAGAHLELHVRRLRRPQVQALEPDQ 348
GS HEA LEL++AD EVRAL+DHL A+LRRPA+VLAGAHLELHVR L RPQV ALEPDQ
Sbjct: 143 GSGHEAQLELVVADPDEVRALVDHLAAVLRRPAQVLAGAHLELHVRGLVRPQVHALEPDQ 322
Query: 347 L 345
L
Sbjct: 323 L 325
Score = 103 bits (220), Expect(2) = 2e-022
Identities = 46/65 (70%), Positives = 49/65 (75%)
Frame = +2 / -3
Query: 329 RPAGQPADRVPEPAPEGDADGARGVRGEPLQALQPGDGGWQEGDRSGLALRDGRRG*VRD 508
R A QPADRVPE AP G ARGVRGEPLQAL+PG GG +GDR GLALR GRR VR
Sbjct: 341 RAARQPADRVPERAPAGGQGRARGVRGEPLQALEPGGGGRPQGDRPGLALRQGRRRRVRV 162
Query: 509 ELHGV 523
ELHG+
Sbjct: 161 ELHGL 147
Score = 35.9 bits (72), Expect(2) = 5e-037
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = +3 / -1
Query: 279 KHSVLVFMRWPNATDG 326
+H VL+F+RWPN TDG
Sbjct: 385 RHPVLLFLRWPNTTDG 338
Score = 20.8 bits (39), Expect(2) = 2e-022
Identities = 6/7 (85%), Positives = 6/7 (85%)
Frame = +1 / -2
Query: 283 TRCWCSC 303
TRC CSC
Sbjct: 381 TRCCCSC 361
>gb|BQ661003.1|BQ661003 HM01H14w HM Hordeum vulgare subsp. vulgare cDNA clone HM01H14
3-PRIME, mRNA sequence
Length = 368
Score = 138 bits (295), Expect(2) = 2e-034
Identities = 56/66 (84%), Positives = 61/66 (92%)
Frame = +3 / -2
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEM 509
GRPASQLIGFQS+HLRA + AHVEFEVSPCKH SRA EDGRKVIDQGSHFV VG+DEFE+
Sbjct: 343 GRPASQLIGFQSVHLRADEAAHVEFEVSPCKHLSRAAEDGRKVIDQGSHFVRVGDDEFEL 164
Query: 510 SFMA*S 527
SFMA*+
Sbjct: 163 SFMA*A 146
Score = 127 bits (272), Expect = 5e-029
Identities = 53/64 (82%), Positives = 54/64 (84%)
Frame = -2 / +3
Query: 520 AMKLISNSSSPTITKCEP*SITFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPISWL 341
AMKL SNSSSPT+TKCEP SIT PSS ARLKCLQGLTSNSTCA A RC LWNPISWL
Sbjct: 153 AMKLNSNSSSPTLTKCEPWSITLRPSSAARLKCLQGLTSNSTCAASSARRCTLWNPISWL 332
Query: 340 AGRP 329
AGRP
Sbjct: 333 AGRP 344
Score = 112 bits (240), Expect = 1e-024
Identities = 48/61 (78%), Positives = 53/61 (86%)
Frame = -1 / +2
Query: 527 GSRHEAHLELILADHHEVRALIDHLPAILRRPAEVLAGAHLELHVRRLRRPQVQALEPDQ 348
GS HEA LEL++AD EVRAL+DHL A+LRRPA+VLAGAHLELHVR L RPQV ALEPDQ
Sbjct: 146 GSGHEAQLELVVADPDEVRALVDHLAAVLRRPAQVLAGAHLELHVRGLVRPQVHALEPDQ 325
Query: 347 L 345
L
Sbjct: 326 L 328
Score = 103 bits (220), Expect = 8e-022
Identities = 46/65 (70%), Positives = 49/65 (75%)
Frame = +2 / -1
Query: 329 RPAGQPADRVPEPAPEGDADGARGVRGEPLQALQPGDGGWQEGDRSGLALRDGRRG*VRD 508
R A QPADRVPE AP G ARGVRGEPLQAL+PG GG +GDR GLALR GRR VR
Sbjct: 344 RAARQPADRVPERAPAGGRGRARGVRGEPLQALEPGGGGRPQGDRPGLALRQGRRRRVRV 165
Query: 509 ELHGV 523
ELHG+
Sbjct: 164 ELHGL 150
Score = 26.7 bits (52), Expect(2) = 2e-034
Identities = 8/9 (88%), Positives = 9/9 (100%)
Frame = +3 / -2
Query: 300 MRWPNATDG 326
+RWPNATDG
Sbjct: 367 LRWPNATDG 341
>gb|BI776777.2|BI776777 EBpi07_SQ001_L15_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ001_L15 5', mRNA sequence
Length = 290
Score = 105 bits (224), Expect(3) = 6e-032
Identities = 40/54 (74%), Positives = 47/54 (87%)
Frame = +3 / +2
Query: 165 ATAGGMASYDVEAIGSETCDRLKFPAVVRVQNHGPMDGKHSVLVFMRWPNATDG 326
A+A G SYDVE +G+E CDRL+FPAVVRVQNHGPMDG+H VL+F+R PNATDG
Sbjct: 77 ASAAGTVSYDVEEMGAEACDRLRFPAVVRVQNHGPMDGRHPVLLFLRGPNATDG 238
Score = 93.6 bits (198), Expect(3) = 4e-028
Identities = 39/54 (72%), Positives = 42/54 (77%)
Frame = -2 / -3
Query: 325 PSVALGQRMNTSTECFPSMGPWFCTRTTAGNLSRSHVSDPIASTS*LAMPPAVA 164
PSVA G R N+ST C PSMGPWFCTRTTAGN S SH S PI+STS*L +P A A
Sbjct: 237 PSVAFGPRRNSSTGCLPSMGPWFCTRTTAGNRSLSHASAPISSTS*LTVPAADA 76
Score = 43.2 bits (88), Expect(3) = 4e-028
Identities = 15/17 (88%), Positives = 15/17 (88%)
Frame = -2 / -3
Query: 379 ALRCRLWNPISWLAGRP 329
A RC LWNPISWLAGRP
Sbjct: 285 ARRCTLWNPISWLAGRP 235
Score = 40.5 bits (82), Expect(3) = 6e-032
Identities = 16/17 (94%), Positives = 17/17 (100%)
Frame = +3 / +2
Query: 330 GRPASQLIGFQSLHLRA 380
GRPASQLIGFQS+HLRA
Sbjct: 236 GRPASQLIGFQSVHLRA 286
Score = 26.7 bits (52), Expect(3) = 6e-032
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = +3 / +2
Query: 105 HRFATKPPPTSNVAGLKAVEATAGGMAS 188
HRFA+K +++G++ ++ATA A+
Sbjct: 5 HRFASKGTKPPSMSGIEGLKATARASAA 88
Score = 23.1 bits (44), Expect(3) = 4e-028
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = -2 / -3
Query: 163 STALRPATLLVGGGFV 116
+ ALRP+ L+ GGFV
Sbjct: 72 AVALRPSMPLMDGGFV 25
>gb|BM372498.2|BM372498 EBpi05_SQ003_C05_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ003_C05 5', mRNA sequence
Length = 505
Score = 101 bits (216), Expect(2) = 9e-031
Identities = 39/62 (62%), Positives = 51/62 (82%)
Frame = +3 / +1
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFATKPPPTSNVAGLKAVEATAGGM 182
RAD +TGYPGRTYRFY+G TV+NFGYGLSYSKYSHRFA+K +++G++ ++ATA
Sbjct: 241 RADLSTGYPGRTYRFYKGKTVYNFGYGLSYSKYSHRFASKGTKPPSMSGIEGLKATARAS 420
Query: 183 AS 188
A+
Sbjct: 421 AA 426
Score = 51.0 bits (105), Expect(2) = 9e-031
Identities = 20/30 (66%), Positives = 24/30 (80%)
Frame = +3 / +1
Query: 165 ATAGGMASYDVEAIGSETCDRLKFPAVVRV 254
A+A G SY VE +G+E CDRL+FPAVVRV
Sbjct: 415 ASAAGTVSYXVEEMGAEACDRLRFPAVVRV 504
>gb|AL512219.1|AL512219 AL512219 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY10M22u 3',
mRNA sequence
Length = 351
Score = 122 bits (262), Expect = 1e-027
Identities = 50/61 (81%), Positives = 55/61 (90%)
Frame = +3 / -2
Query: 345 QLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSHFVMVGEDEFEMSFMA* 524
QLIG QS+HLRA + AHVEFEVSPCKH SRA EDGRKVIDQGSHFV VG+DEFE+SFMA*
Sbjct: 350 QLIGXQSVHLRADEAAHVEFEVSPCKHLSRAAEDGRKVIDQGSHFVRVGDDEFELSFMA* 171
Query: 525 S 527
+
Sbjct: 170 A 168
Score = 112 bits (239), Expect = 2e-024
Identities = 47/59 (79%), Positives = 48/59 (81%)
Frame = -2 / +1
Query: 520 AMKLISNSSSPTITKCEP*SITFLPSSVARLKCLQGLTSNSTCAVCVALRCRLWNPISW 344
AMKL SNSSSPT+TKCEP SIT PSS ARLKCLQGLTSNSTCA A RC LW PISW
Sbjct: 175 AMKLNSNSSSPTLTKCEPWSITLRPSSAARLKCLQGLTSNSTCAASSARRCTLWXPISW 351
Score = 110 bits (234), Expect = 8e-024
Identities = 47/61 (77%), Positives = 52/61 (85%)
Frame = -1 / +3
Query: 527 GSRHEAHLELILADHHEVRALIDHLPAILRRPAEVLAGAHLELHVRRLRRPQVQALEPDQ 348
GS HEA LEL++AD EVRAL+DHL A+LRRPA+VLAGAHLELHVR L RPQV AL PDQ
Sbjct: 168 GSGHEAQLELVVADPDEVRALVDHLAAVLRRPAQVLAGAHLELHVRGLVRPQVHALXPDQ 347
Query: 347 L 345
L
Sbjct: 348 L 350
>gb|BM372501.2|BM372501 EBpi05_SQ003_C08_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ003_C08 5', mRNA sequence
Length = 475
Score = 101 bits (216), Expect(2) = 1e-025
Identities = 39/62 (62%), Positives = 51/62 (82%)
Frame = +3 / +1
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFATKPPPTSNVAGLKAVEATAGGM 182
RAD +TGYPGRTYRFY+G TV+NFGYGLSYSKYSHRFA+K +++G++ ++ATA
Sbjct: 241 RADLSTGYPGRTYRFYKGKTVYNFGYGLSYSKYSHRFASKGTKPPSMSGIEGLKATARAS 420
Query: 183 AS 188
A+
Sbjct: 421 AA 426
Score = 33.6 bits (67), Expect(2) = 1e-025
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +3 / +1
Query: 165 ATAGGMASYDVEAIGSETCD 224
A+A G SYDVE +G+E CD
Sbjct: 415 ASAAGTVSYDVEEMGAEACD 474
>gb|BQ468849.1|BQ468849 HM02I18r HM Hordeum vulgare subsp. vulgare cDNA clone HM02I18
5-PRIME, mRNA sequence
Length = 607
Score = 106 bits (226), Expect = 1e-022
Identities = 40/62 (64%), Positives = 52/62 (83%)
Frame = +3 / +1
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFATKPPPTSNVAGLKAVEATAGGM 182
RADP+TGYPGRTYRFY+G TV+NFGYGLSYSKYSHRFA+K +++G++ ++ATA
Sbjct: 400 RADPSTGYPGRTYRFYKGKTVYNFGYGLSYSKYSHRFASKGTKPPSMSGIEGLKATARAS 579
Query: 183 AS 188
A+
Sbjct: 580 AA 585
>gb|BI960413.1|BI960413 HVSMEn0024I08f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0024I08f, mRNA sequence
Length = 699
Score = 98.7 bits (209), Expect(2) = 1e-021
Identities = 37/59 (62%), Positives = 46/59 (77%)
Frame = +3 / +3
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFATKPPPTSNVAGLKAVEATAGG 179
RADP+TGYPGRTYRFY+G TV+NFGYGLSYSKYSHRFA+K +++G+ + G
Sbjct: 357 RADPSTGYPGRTYRFYKGKTVYNFGYGLSYSKYSHRFASKGTKPPSMSGIGGPQGDGKG 533
Score = 24.0 bits (46), Expect(2) = 1e-021
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = +2 / +2
Query: 230 QVPGGGAGAEPRA 268
+VPGGG GAE A
Sbjct: 599 RVPGGGPGAETTA 637
>gb|CA022183.1|CA022183 HZ42G07r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ42G07
5-PRIME, mRNA sequence
Length = 606
Score = 82.2 bits (173), Expect(2) = 2e-021
Identities = 30/47 (63%), Positives = 37/47 (78%)
Frame = +3 / +1
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFATKPPPTSNV 143
RADPATGYPGR+YRFY+G TV+ FGYGLSYS YS R + P +++
Sbjct: 361 RADPATGYPGRSYRFYQGETVYKFGYGLSYSSYSRRLLSSGTPNTDL 501
Score = 39.1 bits (79), Expect(2) = 2e-021
Identities = 15/25 (60%), Positives = 19/25 (76%)
Frame = +3 / +1
Query: 165 ATAGGMASYDVEAIGSETCDRLKFP 239
A GG+ASY VE IG+ C++LKFP
Sbjct: 532 AEEGGVASYHVEHIGARGCEQLKFP 606
>gb|AV927333.1|AV927333 AV927333 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd1j19 3', mRNA sequence
Length = 657
Score = 51.5 bits (106), Expect(3) = 3e-021
Identities = 20/49 (40%), Positives = 30/49 (61%)
Frame = +3 / -3
Query: 330 GRPASQLIGFQSLHLRATQTAHVEFEVSPCKHFSRATEDGRKVIDQGSH 476
G P QL+GF+ ++ A + +V V PCK+ S A +GR+V+ GSH
Sbjct: 349 GFPLKQLVGFERVYTAAGSSKNVAITVDPCKYMSAANTEGRRVLLLGSH 203
Score = 48.3 bits (99), Expect(3) = 3e-021
Identities = 18/31 (58%), Positives = 20/31 (64%)
Frame = +3 / -3
Query: 33 RTYRFYRGPTVFNFGYGLSYSKYSHRFATKP 125
RTYRFY V+ F YGLSYSKYS+ P
Sbjct: 655 RTYRFYTXEVVYGFXYGLSYSKYSYNIVQAP 563
Score = 38.6 bits (78), Expect(3) = 3e-021
Identities = 15/37 (40%), Positives = 22/37 (59%)
Frame = +3 / -3
Query: 216 TCDRLKFPAVVRVQNHGPMDGKHSVLVFMRWPNATDG 326
+C+ L F + V N G MDG H+VL+F R ++ G
Sbjct: 457 SCESLVFSVHISVANXGAMDGSHAVLLFARSKSSVPG 347
>gb|BM372495.2|BM372495 EBpi05_SQ003_C02_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ003_C02 5', mRNA sequence
Length = 418
Score = 100 bits (214), Expect = 5e-021
Identities = 38/57 (66%), Positives = 49/57 (85%)
Frame = +3 / +1
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFATKPPPTSNVAGLKAVEATA 173
RAD +TGYPGRTYRFY+G TV+NFGYGLSYSKYSHRFA+K +++G++ ++ATA
Sbjct: 241 RADLSTGYPGRTYRFYKGKTVYNFGYGLSYSKYSHRFASKGTKPPSMSGIEGLKATA 411
>gb|BM372479.2|BM372479 EBpi05_SQ003_B09_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ003_B09 5', mRNA sequence
Length = 418
Score = 100 bits (214), Expect = 5e-021
Identities = 38/57 (66%), Positives = 49/57 (85%)
Frame = +3 / +1
Query: 3 RADPATGYPGRTYRFYRGPTVFNFGYGLSYSKYSHRFATKPPPTSNVAGLKAVEATA 173
RAD +TGYPGRTYRFY+G TV+NFGYGLSYSKYSHRFA+K +++G++ ++ATA
Sbjct: 241 RADLSTGYPGRTYRFYKGKTVYNFGYGLSYSKYSHRFASKGTKPPSMSGIEGLKATA 411
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 201,001,835
Number of Sequences: 312970
Number of extensions: 3246276
Number of successful extensions: 96525
Number of sequences better than 1.0e-020: 36
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5554858270
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)