TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440444.2.1
(237 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL511547.1|AL511547 AL511547 Hordeum vulgare Barke devel... 139 2e-033
gb|AV925753.1|AV925753 AV925753 K. Sato unpublished cDNA li... 139 2e-033
gb|AV930889.1|AV930889 AV930889 K. Sato unpublished cDNA li... 139 2e-033
gb|BU972244.1|BU972244 HB21B09r BC Hordeum vulgare subsp. v... 139 2e-033
gb|BU972337.1|BU972337 HB21F19r BC Hordeum vulgare subsp. v... 139 2e-033
gb|BU982554.1|BU982554 HA26O10r HA Hordeum vulgare subsp. v... 132 5e-031
gb|BQ661228.1|BQ661228 HM02I18u HM Hordeum vulgare subsp. v... 123 1e-028
gb|AL511138.1|AL511138 AL511138 Hordeum vulgare Barke devel... 123 2e-028
gb|BM100425.1|BM100425 EBma01_SQ005_C23_R maternal, 4 DPA, ... 123 2e-028
gb|BQ460471.1|BQ460471 HA09H11r HA Hordeum vulgare subsp. v... 123 2e-028
gb|BQ657146.1|BQ657146 HA09H11u HA Hordeum vulgare subsp. v... 123 2e-028
gb|BU988389.1|BU988389 HF17H23r HF Hordeum vulgare subsp. v... 119 3e-027
gb|BQ661003.1|BQ661003 HM01H14w HM Hordeum vulgare subsp. v... 111 5e-025
gb|BU971682.1|BU971682 HB19F13r BC Hordeum vulgare subsp. v... 100 1e-021
>gb|AL511547.1|AL511547 AL511547 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY08G20u 3',
mRNA sequence
Length = 566
Score = 139 bits (299), Expect = 2e-033
Identities = 56/78 (71%), Positives = 60/78 (76%)
Frame = -3 / -1
Query: 235 KHSVLMFLRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKVID 56
KHSVLMFLRWPN GRP SQLIGFR+QHLKAGE A L FD+ PC+HFSR R DG+KVID
Sbjct: 359 KHSVLMFLRWPNATGGRPVSQLIGFRTQHLKAGEKASLTFDVRPCEHFSRAREDGKKVID 180
Query: 55 IGSHFFMVDNHEMEIRFE 2
GSHF V E EI FE
Sbjct: 179 AGSHFLKVGKDEHEISFE 126
Score = 123 bits (264), Expect = 1e-028
Identities = 51/78 (65%), Positives = 58/78 (74%)
Frame = +3 / +1
Query: 3 SNLISISWLSTMKK*DPISITFLPSALTLEKCLHGLMSNLSLAVSPALRCWLLNPISWLA 182
S LIS S T++K +P SITFLPS+L L KC HGL SN+ LA SPALRCW+ NPISWL
Sbjct: 127 SKLISCSSFPTLRKCEPASITFLPSSLALLKCSHGLTSNVRLAFSPALRCWVRNPISWLT 306
Query: 183 GRPCFVFGQRRNMSTECF 236
GRP G RRN+STECF
Sbjct: 307 GRPPVALGHRRNISTECF 360
>gb|AV925753.1|AV925753 AV925753 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd25k17 5', mRNA sequence
Length = 582
Score = 139 bits (299), Expect = 2e-033
Identities = 56/78 (71%), Positives = 60/78 (76%)
Frame = -3 / +3
Query: 235 KHSVLMFLRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKVID 56
KHSVLMFLRWPN GRP SQLIGFR+QHLKAGE A L FD+ PC+HFSR R DG+KVID
Sbjct: 309 KHSVLMFLRWPNATGGRPVSQLIGFRTQHLKAGEKASLTFDVRPCEHFSRAREDGKKVID 488
Query: 55 IGSHFFMVDNHEMEIRFE 2
GSHF V E EI FE
Sbjct: 489 AGSHFLKVGKDEHEISFE 542
Score = 123 bits (264), Expect = 1e-028
Identities = 51/78 (65%), Positives = 58/78 (74%)
Frame = +3 / -3
Query: 3 SNLISISWLSTMKK*DPISITFLPSALTLEKCLHGLMSNLSLAVSPALRCWLLNPISWLA 182
S LIS S T++K +P SITFLPS+L L KC HGL SN+ LA SPALRCW+ NPISWL
Sbjct: 541 SKLISCSSFPTLRKCEPASITFLPSSLALLKCSHGLTSNVRLAFSPALRCWVRNPISWLT 362
Query: 183 GRPCFVFGQRRNMSTECF 236
GRP G RRN+STECF
Sbjct: 361 GRPPVALGHRRNISTECF 308
>gb|AV930889.1|AV930889 AV930889 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd25k17 3', mRNA sequence
Length = 673
Score = 139 bits (299), Expect = 2e-033
Identities = 56/78 (71%), Positives = 60/78 (76%)
Frame = -3 / -3
Query: 235 KHSVLMFLRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKVID 56
KHSVLMFLRWPN GRP SQLIGFR+QHLKAGE A L FD+ PC+HFSR R DG+KVID
Sbjct: 416 KHSVLMFLRWPNATGGRPVSQLIGFRTQHLKAGEKASLTFDVRPCEHFSRAREDGKKVID 237
Query: 55 IGSHFFMVDNHEMEIRFE 2
GSHF V E EI FE
Sbjct: 236 AGSHFLKVGKDEHEISFE 183
Score = 123 bits (264), Expect = 1e-028
Identities = 51/78 (65%), Positives = 58/78 (74%)
Frame = +3 / +1
Query: 3 SNLISISWLSTMKK*DPISITFLPSALTLEKCLHGLMSNLSLAVSPALRCWLLNPISWLA 182
S LIS S T++K +P SITFLPS+L L KC HGL SN+ LA SPALRCW+ NPISWL
Sbjct: 184 SKLISCSSFPTLRKCEPASITFLPSSLALLKCSHGLTSNVRLAFSPALRCWVRNPISWLT 363
Query: 183 GRPCFVFGQRRNMSTECF 236
GRP G RRN+STECF
Sbjct: 364 GRPPVALGHRRNISTECF 417
>gb|BU972244.1|BU972244 HB21B09r BC Hordeum vulgare subsp. vulgare cDNA clone HB21B09
5-PRIME, mRNA sequence
Length = 513
Score = 139 bits (299), Expect = 2e-033
Identities = 56/78 (71%), Positives = 60/78 (76%)
Frame = -3 / +3
Query: 235 KHSVLMFLRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKVID 56
KHSVLMFLRWPN GRP SQLIGFR+QHLKAGE A L FD+ PC+HFSR R DG+KVID
Sbjct: 63 KHSVLMFLRWPNATGGRPVSQLIGFRTQHLKAGEKASLTFDVRPCEHFSRAREDGKKVID 242
Query: 55 IGSHFFMVDNHEMEIRFE 2
GSHF V E EI FE
Sbjct: 243 AGSHFLKVGKDEHEISFE 296
Score = 123 bits (264), Expect = 1e-028
Identities = 51/78 (65%), Positives = 58/78 (74%)
Frame = +3 / -3
Query: 3 SNLISISWLSTMKK*DPISITFLPSALTLEKCLHGLMSNLSLAVSPALRCWLLNPISWLA 182
S LIS S T++K +P SITFLPS+L L KC HGL SN+ LA SPALRCW+ NPISWL
Sbjct: 295 SKLISCSSFPTLRKCEPASITFLPSSLALLKCSHGLTSNVRLAFSPALRCWVRNPISWLT 116
Query: 183 GRPCFVFGQRRNMSTECF 236
GRP G RRN+STECF
Sbjct: 115 GRPPVALGHRRNISTECF 62
>gb|BU972337.1|BU972337 HB21F19r BC Hordeum vulgare subsp. vulgare cDNA clone HB21F19
5-PRIME, mRNA sequence
Length = 513
Score = 139 bits (299), Expect = 2e-033
Identities = 56/78 (71%), Positives = 60/78 (76%)
Frame = -3 / +3
Query: 235 KHSVLMFLRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKVID 56
KHSVLMFLRWPN GRP SQLIGFR+QHLKAGE A L FD+ PC+HFSR R DG+KVID
Sbjct: 63 KHSVLMFLRWPNATGGRPVSQLIGFRTQHLKAGEKASLTFDVRPCEHFSRAREDGKKVID 242
Query: 55 IGSHFFMVDNHEMEIRFE 2
GSHF V E EI FE
Sbjct: 243 AGSHFLKVGKDEHEISFE 296
Score = 123 bits (264), Expect = 1e-028
Identities = 51/78 (65%), Positives = 58/78 (74%)
Frame = +3 / -3
Query: 3 SNLISISWLSTMKK*DPISITFLPSALTLEKCLHGLMSNLSLAVSPALRCWLLNPISWLA 182
S LIS S T++K +P SITFLPS+L L KC HGL SN+ LA SPALRCW+ NPISWL
Sbjct: 295 SKLISCSSFPTLRKCEPASITFLPSSLALLKCSHGLTSNVRLAFSPALRCWVRNPISWLT 116
Query: 183 GRPCFVFGQRRNMSTECF 236
GRP G RRN+STECF
Sbjct: 115 GRPPVALGHRRNISTECF 62
>gb|BU982554.1|BU982554 HA26O10r HA Hordeum vulgare subsp. vulgare cDNA clone HA26O10
5-PRIME, mRNA sequence
Length = 609
Score = 132 bits (282), Expect = 5e-031
Identities = 51/78 (65%), Positives = 60/78 (76%)
Frame = -3 / +1
Query: 235 KHSVLMFLRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKVID 56
KHSVL+FLRWPN GRPASQL+GF+SQHL++GE A L FD+SPC+H SR R DG+ VID
Sbjct: 337 KHSVLLFLRWPNATGGRPASQLVGFQSQHLESGEKASLTFDVSPCEHLSRAREDGKMVID 516
Query: 55 IGSHFFMVDNHEMEIRFE 2
SHF VD E EI F+
Sbjct: 517 RASHFLFVDEDEAEISFD 570
Score = 111 bits (237), Expect = 7e-025
Identities = 48/78 (61%), Positives = 54/78 (69%)
Frame = +3 / -2
Query: 3 SNLISISWLSTMKK*DPISITFLPSALTLEKCLHGLMSNLSLAVSPALRCWLLNPISWLA 182
S LIS S ST +K + +SIT LPS+L L KC HGL SN+ LA SP RCWL NP SWLA
Sbjct: 569 SKLISASSSSTKRKCEALSITILPSSLALLKCSHGLTSNVRLAFSPDSRCWLWNPTSWLA 390
Query: 183 GRPCFVFGQRRNMSTECF 236
GRP G RRN +TECF
Sbjct: 389 GRPPVALGHRRNNNTECF 336
>gb|BQ661228.1|BQ661228 HM02I18u HM Hordeum vulgare subsp. vulgare cDNA clone HM02I18
3-PRIME, mRNA sequence
Length = 385
Score = 123 bits (264), Expect = 1e-028
Identities = 48/77 (62%), Positives = 58/77 (75%)
Frame = -3 / -1
Query: 235 KHSVLMFLRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKVID 56
+H VL+FLRWPNT GRPASQLIGF+S HL+A + A + F++SPCKH SR DGRKVID
Sbjct: 385 RHPVLLFLRWPNTTDGRPASQLIGFQSVHLRADKAAHVEFEVSPCKHLSRAAEDGRKVID 206
Query: 55 IGSHFFMVDNHEMEIRF 5
GSHF V + E E+ F
Sbjct: 205 QGSHFVRVGDDEFELSF 155
>gb|AL511138.1|AL511138 AL511138 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY07B24u 3',
mRNA sequence
Length = 440
Score = 123 bits (263), Expect = 2e-028
Identities = 48/77 (62%), Positives = 57/77 (74%)
Frame = -3 / -1
Query: 235 KHSVLMFLRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKVID 56
+H VL+FLRWPN GRPASQLIGF+S HL+A E A + F++SPCKH SR DGRKVID
Sbjct: 419 RHPVLLFLRWPNATDGRPASQLIGFQSVHLRADEAAHVEFEVSPCKHLSRAAEDGRKVID 240
Query: 55 IGSHFFMVDNHEMEIRF 5
GSHF V + E E+ F
Sbjct: 239 QGSHFVRVGDDEFELSF 189
>gb|BM100425.1|BM100425 EBma01_SQ005_C23_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ005_C23 5', mRNA sequence
Length = 426
Score = 123 bits (263), Expect = 2e-028
Identities = 48/77 (62%), Positives = 57/77 (74%)
Frame = -3 / +3
Query: 235 KHSVLMFLRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKVID 56
+H VL+FLRWPN GRPASQLIGF+S HL+A E A + F++SPCKH SR DGRKVID
Sbjct: 36 RHPVLLFLRWPNATDGRPASQLIGFQSVHLRADEAAHVEFEVSPCKHLSRAAEDGRKVID 215
Query: 55 IGSHFFMVDNHEMEIRF 5
GSHF V + E E+ F
Sbjct: 216 QGSHFVRVGDDEFELSF 266
>gb|BQ460471.1|BQ460471 HA09H11r HA Hordeum vulgare subsp. vulgare cDNA clone HA09H11
5-PRIME, mRNA sequence
Length = 576
Score = 123 bits (263), Expect = 2e-028
Identities = 48/77 (62%), Positives = 57/77 (74%)
Frame = -3 / +2
Query: 235 KHSVLMFLRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKVID 56
+H VL+FLRWPN GRPASQLIGF+S HL+A E A + F++SPCKH SR DGRKVID
Sbjct: 296 RHPVLLFLRWPNATDGRPASQLIGFQSVHLRADEAAHVEFEVSPCKHLSRAAEDGRKVID 475
Query: 55 IGSHFFMVDNHEMEIRF 5
GSHF V + E E+ F
Sbjct: 476 QGSHFVRVGDDEFELSF 526
>gb|BQ657146.1|BQ657146 HA09H11u HA Hordeum vulgare subsp. vulgare cDNA clone HA09H11
3-PRIME, mRNA sequence
Length = 521
Score = 123 bits (263), Expect = 2e-028
Identities = 48/77 (62%), Positives = 57/77 (74%)
Frame = -3 / -2
Query: 235 KHSVLMFLRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKVID 56
+H VL+FLRWPN GRPASQLIGF+S HL+A E A + F++SPCKH SR DGRKVID
Sbjct: 373 RHPVLLFLRWPNATDGRPASQLIGFQSVHLRADEAAHVEFEVSPCKHLSRAAEDGRKVID 194
Query: 55 IGSHFFMVDNHEMEIRF 5
GSHF V + E E+ F
Sbjct: 193 QGSHFVRVGDDEFELSF 143
>gb|BU988389.1|BU988389 HF17H23r HF Hordeum vulgare subsp. vulgare cDNA clone HF17H23
5-PRIME, mRNA sequence
Length = 426
Score = 119 bits (254), Expect = 3e-027
Identities = 46/77 (59%), Positives = 56/77 (72%)
Frame = -3 / +1
Query: 235 KHSVLMFLRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKVID 56
+H VL+FLRWPN GRPASQLIGF+S HL+A + + F++SPCKH SR DGRKVID
Sbjct: 37 RHPVLLFLRWPNATDGRPASQLIGFQSVHLRADDVFHVEFEVSPCKHLSRAAEDGRKVID 216
Query: 55 IGSHFFMVDNHEMEIRF 5
GSHF V + E E+ F
Sbjct: 217 QGSHFVRVGDDEFELSF 267
>gb|BQ661003.1|BQ661003 HM01H14w HM Hordeum vulgare subsp. vulgare cDNA clone HM01H14
3-PRIME, mRNA sequence
Length = 368
Score = 111 bits (238), Expect = 5e-025
Identities = 44/70 (62%), Positives = 51/70 (72%)
Frame = -3 / -2
Query: 214 LRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKVIDIGSHFFM 35
LRWPN GRPASQLIGF+S HL+A E A + F++SPCKH SR DGRKVID GSHF
Sbjct: 367 LRWPNATDGRPASQLIGFQSVHLRADEAAHVEFEVSPCKHLSRAAEDGRKVIDQGSHFVR 188
Query: 34 VDNHEMEIRF 5
V + E E+ F
Sbjct: 187 VGDDEFELSF 158
>gb|BU971682.1|BU971682 HB19F13r BC Hordeum vulgare subsp. vulgare cDNA clone HB19F13
5-PRIME, mRNA sequence
Length = 410
Score = 100 bits (214), Expect = 1e-021
Identities = 38/58 (65%), Positives = 45/58 (77%)
Frame = -3 / +3
Query: 235 KHSVLMFLRWPNTKQGRPASQLIGFRSQHLKAGETAKLRFDISPCKHFSRVRADGRKV 62
+H VL+FLRWPN GRPASQLIGF+S HL+A E A + F++SPCKH SR DGRKV
Sbjct: 237 RHPVLLFLRWPNATDGRPASQLIGFQSVHLRADEAAHVEFEVSPCKHLSRAAEDGRKV 410
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 144,738,091
Number of Sequences: 312970
Number of extensions: 2435956
Number of successful extensions: 312794
Number of sequences better than 1.0e-020: 17
length of database: 58,378,179
effective HSP length: 47
effective length of database: 43,668,589
effective search space used: 1353726259
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)