TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2437629.2.2
(1041 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ460841.1|BJ460841 BJ460841 K. Sato unpublished cDNA li... 147 3e-051
gb|BI948762.1|BI948762 HVSMEl0010L20f Hordeum vulgare spike... 136 2e-039
gb|CB858531.1|CB858531 HI07G08w HI Hordeum vulgare subsp. v... 118 2e-036
gb|BJ455168.1|BJ455168 BJ455168 K. Sato unpublished cDNA li... 122 3e-032
gb|BJ473611.1|BJ473611 BJ473611 K. Sato unpublished cDNA li... 122 3e-032
gb|BI780615.2|BI780615 EBes01_SQ001_H19_R embryo sac, 4-6 D... 122 3e-028
gb|BJ453298.1|BJ453298 BJ453298 K. Sato unpublished cDNA li... 123 1e-027
>gb|BJ460841.1|BJ460841 BJ460841 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak42g23 3', mRNA sequence
Length = 644
Score = 147 bits (315), Expect(3) = 3e-051
Identities = 60/69 (86%), Positives = 64/69 (92%)
Frame = +1 / -1
Query: 4 AIIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLS 183
AIIRHPR+GEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFL+NS RPNLS
Sbjct: 530 AIIRHPRVGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLLNSEEIIRPNLS 351
Query: 184 IREGIGMVV 210
IREGI ++V
Sbjct: 350 IREGIEIIV 324
Score = 120 bits (257), Expect(3) = 9e-045
Identities = 53/70 (75%), Positives = 59/70 (84%)
Frame = -2 / +1
Query: 212 QTTIPIPSRIDKLGLXXXXEFTRNISPMYR*FVGTYTLHSSSSPLSV*RTTVDVMNPNAY 33
+T I IPSR+DK GL EF+RNISP+YR*FVGT TLH+SSSPLSV RTTVDV+NPNAY
Sbjct: 322 ETIISIPSRMDKFGLMISSEFSRNISPIYR*FVGT*TLHNSSSPLSVWRTTVDVINPNAY 501
Query: 32 SPIRG*RIIA 3
SP RG RIIA
Sbjct: 502 SPTRGCRIIA 531
Score = 95.0 bits (201), Expect(2) = 5e-025
Identities = 37/68 (54%), Positives = 52/68 (76%)
Frame = -1 / +3
Query: 210 NYHTDSFSNR*IRSXXXXXVHQKYITDVQMICWHIHTTQFFVAFVSLKDHR*CYESKCIF 31
N + +SFSN *+ S V QKYIT++ MIC HI+TTQF + FV L+++ *CY+S+C+F
Sbjct: 324 NDNFNSFSNG*VWSDDLFRVQQKYITNI*MICRHINTTQFLITFVCLENNS*CYKSECVF 503
Query: 30 TNSWMTNY 7
TN+WM+NY
Sbjct: 504 TNTWMSNY 527
Score = 72.5 bits (152), Expect(3) = 9e-045
Identities = 30/39 (76%), Positives = 33/39 (84%)
Frame = -3 / +1
Query: 499 HHVILFRRMFWLFRGMGSSDAIT*GMVIPPETIISAETK 383
H +ILFRRM WLF GMGSSDAIT GMVIPPETIIS ++
Sbjct: 232 HVIILFRRMLWLFLGMGSSDAITCGMVIPPETIISIPSR 348
Score = 68.0 bits (142), Expect(3) = 3e-051
Identities = 28/32 (87%), Positives = 32/32 (100%)
Frame = +3 / -1
Query: 396 EIIVSGGMTMPQVIASLEPMPRKSQNIRLNRM 491
EIIVSGGMTMPQVIASL+P+PRKSQ+IRLNR+
Sbjct: 335 EIIVSGGMTMPQVIASLDPIPRKSQSIRLNRI 240
Score = 42.3 bits (86), Expect(2) = 5e-025
Identities = 17/32 (53%), Positives = 22/32 (68%)
Frame = -2 / +3
Query: 491 HSI*TNVLAFPWHGLQ*CNHLRHGHPS*NNYF 396
+SI* + LA PW G+ CN+L HGH S N+ F
Sbjct: 240 YSI*ADALALPWDGV*RCNYLWHGHSSRNDNF 335
Score = 29.9 bits (59), Expect(3) = 3e-051
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +2 / -1
Query: 683 EASC*CPGILFPHKLCYSLC 742
EAS CPGIL C+SLC
Sbjct: 131 EASYRCPGILLYGTCCFSLC 72
Score = 29.9 bits (59), Expect(3) = 9e-045
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -1 / +1
Query: 753 PNTPHNE*HNLCGNNIPGHQHDASPAL 673
P + H E ++ N+IPGH++DAS L
Sbjct: 61 PVSKHREKQHVPYNSIPGHRYDASVQL 141
>gb|BI948762.1|BI948762 HVSMEl0010L20f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0010L20f, mRNA sequence
Length = 834
Score = 136 bits (292), Expect(2) = 2e-039
Identities = 56/69 (81%), Positives = 61/69 (88%)
Frame = +1 / +2
Query: 4 AIIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLS 183
AII HPR GEYAFGFITS+++LQTDKGDEELCSVYVPTNHL IGDIFLVNS RPNLS
Sbjct: 263 AIISHPRAGEYAFGFITSSMILQTDKGDEELCSVYVPTNHLCIGDIFLVNSAEIIRPNLS 442
Query: 184 IREGIGMVV 210
IREGI ++V
Sbjct: 443 IREGIEIIV 469
Score = 107 bits (228), Expect(2) = 3e-029
Identities = 48/70 (68%), Positives = 56/70 (80%)
Frame = -2 / -1
Query: 212 QTTIPIPSRIDKLGLXXXXEFTRNISPMYR*FVGTYTLHSSSSPLSV*RTTVDVMNPNAY 33
+T I IPSR+D+LGL EFT+ ISP++R* VGT TLHSSSSPLSV*R +DV+NPNAY
Sbjct: 471 ETIISIPSRMDRLGLIISAEFTKKISPIHR*LVGT*TLHSSSSPLSV*RIMLDVINPNAY 292
Query: 32 SPIRG*RIIA 3
SP RG IIA
Sbjct: 291 SPARGWLIIA 262
Score = 90.4 bits (191), Expect(2) = 1e-022
Identities = 37/69 (53%), Positives = 46/69 (66%)
Frame = -1 / -3
Query: 210 NYHTDSFSNR*IRSXXXXXVHQKYITDVQMICWHIHTTQFFVAFVSLKDHR*CYESKCIF 31
N + FSN + VHQK IT+ MI WHI TTQFF+ FVSLK+H CY+SKC+F
Sbjct: 469 NNYLYPFSNGQVGPYNLCRVHQKDITNT*MIGWHIDTTQFFITFVSLKNHARCYKSKCVF 290
Query: 30 TNSWMTNYC 4
T++ M NYC
Sbjct: 289 TSTGMANYC 263
Score = 49.2 bits (101), Expect(2) = 2e-039
Identities = 20/32 (62%), Positives = 26/32 (81%)
Frame = +3 / +2
Query: 396 EIIVSGGMTMPQVIASLEPMPRKSQNIRLNRM 491
EIIVSGGMTMP+VI +L P P ++Q RL+R+
Sbjct: 458 EIIVSGGMTMPEVITALGPAPDENQGARLDRV 553
Score = 44.6 bits (91), Expect(2) = 3e-029
Identities = 18/31 (58%), Positives = 19/31 (61%)
Frame = -2 / -3
Query: 491 HSI*TNVLAFPWHGLQ*CNHLRHGHPS*NNY 399
HS+*T L W G C HLRHGH S NNY
Sbjct: 553 HSV*TGPLVLIWRGS*SCYHLRHGHSSRNNY 461
Score = 39.6 bits (80), Expect(2) = 1e-022
Identities = 18/35 (51%), Positives = 21/35 (60%)
Frame = -3 / -1
Query: 487 LFRRMFWLFRGMGSSDAIT*GMVIPPETIISAETK 383
L +R W G G IT GMVIPPETIIS ++
Sbjct: 549 LSKRAPWFSSGAGPRAVITSGMVIPPETIISIPSR 445
>gb|CB858531.1|CB858531 HI07G08w HI Hordeum vulgare subsp. vulgare cDNA clone HI07G08
3-PRIME, mRNA sequence
Length = 580
Score = 118 bits (253), Expect(2) = 2e-036
Identities = 48/58 (82%), Positives = 53/58 (91%)
Frame = +1 / -3
Query: 37 AFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLSIREGIGMVV 210
AFGFITS+++LQTDKGDEELCSVYVPTNHLYIGDIFLVNS RPNLSIREGI ++V
Sbjct: 578 AFGFITSSMILQTDKGDEELCSVYVPTNHLYIGDIFLVNSAEIIRPNLSIREGIEIIV 405
Score = 94.1 bits (199), Expect(2) = 1e-026
Identities = 41/59 (69%), Positives = 48/59 (81%)
Frame = -2 / +1
Query: 212 QTTIPIPSRIDKLGLXXXXEFTRNISPMYR*FVGTYTLHSSSSPLSV*RTTVDVMNPNA 36
+T I IPSR+D+LGL EFT+ ISP+YR* VGT TLHSSSSPLSV*R +DV+NPNA
Sbjct: 403 ETIISIPSRMDRLGLIISAEFTKKISPIYR*LVGT*TLHSSSSPLSV*RIMLDVINPNA 579
Score = 56.5 bits (117), Expect(2) = 2e-036
Identities = 24/32 (75%), Positives = 26/32 (81%)
Frame = +3 / -3
Query: 396 EIIVSGGMTMPQVIASLEPMPRKSQNIRLNRM 491
EIIVSGGMTMPQVI +L P P K+Q RLNRM
Sbjct: 416 EIIVSGGMTMPQVITALGPAPDKNQGTRLNRM 321
Score = 48.3 bits (99), Expect(2) = 1e-026
Identities = 20/37 (54%), Positives = 25/37 (67%)
Frame = -3 / +1
Query: 493 VILFRRMFWLFRGMGSSDAIT*GMVIPPETIISAETK 383
+ILF+R+ W G G IT GMVIPPETIIS ++
Sbjct: 319 IILFKRVPWFLSGAGPRAVITCGMVIPPETIISIPSR 429
>gb|BJ455168.1|BJ455168 BJ455168 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak35h23 3', mRNA sequence
Length = 697
Score = 122 bits (260), Expect(2) = 3e-032
Identities = 51/68 (75%), Positives = 57/68 (83%)
Frame = +1 / -2
Query: 7 IIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLSI 186
IIRHPRIGEYAFGFITS+V LQ+ G E+L VYVPTNHLYIGDIF+VNS RPNLS+
Sbjct: 468 IIRHPRIGEYAFGFITSSVSLQSYSGQEDLYCVYVPTNHLYIGDIFMVNSKDVIRPNLSV 289
Query: 187 REGIGMVV 210
REGI +VV
Sbjct: 288 REGIEIVV 265
Score = 39.6 bits (80), Expect(2) = 3e-032
Identities = 13/21 (61%), Positives = 20/21 (95%)
Frame = +3 / -2
Query: 396 EIIVSGGMTMPQVIASLEPMP 458
EI+VSGGM+MPQ++++L+P P
Sbjct: 276 EIVVSGGMSMPQILSTLDPHP 214
>gb|BJ473611.1|BJ473611 BJ473611 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal15k07 3', mRNA sequence
Length = 699
Score = 122 bits (260), Expect(2) = 3e-032
Identities = 51/68 (75%), Positives = 57/68 (83%)
Frame = +1 / -1
Query: 7 IIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLSI 186
IIRHPRIGEYAFGFITS+V LQ+ G E+L VYVPTNHLYIGDIF+VNS RPNLS+
Sbjct: 468 IIRHPRIGEYAFGFITSSVSLQSYSGQEDLYCVYVPTNHLYIGDIFMVNSKDVIRPNLSV 289
Query: 187 REGIGMVV 210
REGI +VV
Sbjct: 288 REGIEIVV 265
Score = 39.6 bits (80), Expect(2) = 3e-032
Identities = 13/21 (61%), Positives = 20/21 (95%)
Frame = +3 / -1
Query: 396 EIIVSGGMTMPQVIASLEPMP 458
EI+VSGGM+MPQ++++L+P P
Sbjct: 276 EIVVSGGMSMPQILSTLDPHP 214
>gb|BI780615.2|BI780615 EBes01_SQ001_H19_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ001_H19 5', mRNA sequence
Length = 527
Score = 122 bits (260), Expect(2) = 3e-028
Identities = 51/68 (75%), Positives = 57/68 (83%)
Frame = +1 / +2
Query: 7 IIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLSI 186
IIRHPRIGEYAFGFITS+V LQ+ G E+L VYVPTNHLYIGDIF+VNS RPNLS+
Sbjct: 302 IIRHPRIGEYAFGFITSSVSLQSYSGQEDLYCVYVPTNHLYIGDIFMVNSKDVIRPNLSV 481
Query: 187 REGIGMVV 210
REGI +VV
Sbjct: 482 REGIEIVV 505
Score = 25.8 bits (50), Expect(2) = 3e-028
Identities = 9/11 (81%), Positives = 11/11 (100%)
Frame = +3 / +2
Query: 396 EIIVSGGMTMP 428
EI+VSGGM+MP
Sbjct: 494 EIVVSGGMSMP 526
>gb|BJ453298.1|BJ453298 BJ453298 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak42g23 5', mRNA sequence
Length = 633
Score = 123 bits (264), Expect = 1e-027
Identities = 50/56 (89%), Positives = 52/56 (92%)
Frame = +1 / +2
Query: 4 AIIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXR 171
AIIRHPR+GEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFL+NS R
Sbjct: 464 AIIRHPRVGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLLNSEEIIR 631
Score = 101 bits (216), Expect = 5e-021
Identities = 45/57 (78%), Positives = 49/57 (85%)
Frame = -2 / -1
Query: 173 GLXXXXEFTRNISPMYR*FVGTYTLHSSSSPLSV*RTTVDVMNPNAYSPIRG*RIIA 3
GL EF+RNISP+YR*FVGT TLH+SSSPLSV RTTVDV+NPNAYSP RG RIIA
Sbjct: 633 GLMISSEFSRNISPIYR*FVGT*TLHNSSSPLSVWRTTVDVINPNAYSPTRGCRIIA 463
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 442,484,896
Number of Sequences: 312970
Number of extensions: 6448407
Number of successful extensions: 47663
Number of sequences better than 1.0e-020: 10
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 12378499266
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)