TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419417.2.5
(733 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL505468.1|AL505468 AL505468 Hordeum vulgare Barke roots... 247 5e-065
gb|CA023147.1|CA023147 HZ45F22r HZ Hordeum vulgare subsp. v... 166 9e-041
gb|BF254222.2|BF254222 HVSMEf0003F20f Hordeum vulgare seedl... 155 2e-037
gb|CB858437.1|CB858437 HI07A02w HI Hordeum vulgare subsp. v... 69 2e-023
gb|BF623927.2|BF623927 HVSMEa0007M14f Hordeum vulgare seedl... 70 8e-023
gb|BJ467618.1|BJ467618 BJ467618 K. Sato unpublished cDNA li... 71 1e-022
gb|BU977487.1|BU977487 HA11I05r HA Hordeum vulgare subsp. v... 68 1e-022
gb|BU968461.1|BU968461 HB07J14r BC Hordeum vulgare subsp. v... 68 1e-022
gb|BU968389.1|BU968389 HB07F20r BC Hordeum vulgare subsp. v... 68 1e-022
gb|BI951308.1|BI951308 HVSMEl0025H09f Hordeum vulgare spike... 65 2e-022
gb|BE601818.2|BE601818 HVSMEh0099K11f Hordeum vulgare 5-45 ... 65 5e-022
gb|BQ659818.1|BQ659818 HF01M24w HF Hordeum vulgare subsp. v... 65 9e-022
gb|BQ755347.1|BQ755347 EBed07_SQ002_J07_R endosperm, 28 DPA... 65 9e-022
gb|BE601804.3|BE601804 HVSMEh0099J17f Hordeum vulgare 5-45 ... 65 9e-022
gb|BU986792.1|BU986792 HF12O16r HF Hordeum vulgare subsp. v... 65 9e-022
gb|BQ466282.1|BQ466282 HT02B16r HT Hordeum vulgare subsp. v... 65 9e-022
gb|BU984559.1|BU984559 HF04E16r HF Hordeum vulgare subsp. v... 65 9e-022
gb|BQ472509.1|BQ472509 HB09F20T BC Hordeum vulgare subsp. v... 64 2e-021
gb|BE454083.2|BE454083 HVSMEh0080E16f Hordeum vulgare 5-45 ... 61 4e-021
gb|BI947748.1|BI947748 HVSMEl0006L16f Hordeum vulgare spike... 65 6e-021
gb|CV053661.1|CV053661 BNEL101g2 Barley EST endosperm libra... 64 6e-021
gb|BI949909.1|BI949909 HVSMEl0016O06f Hordeum vulgare spike... 65 6e-021
>gb|AL505468.1|AL505468 AL505468 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW08J03V 5', mRNA sequence
Length = 545
Score = 247 bits (534), Expect = 5e-065
Identities = 97/116 (83%), Positives = 106/116 (91%)
Frame = +2 / +2
Query: 5 RKLMNYVKERYNSPTVYITENGMDDGNSPFTSIQDALKDSKRIKYHNGYLNNVAASIKED 184
R LMNYVK RYNSP +YITENGMDD NSPF +++DALKD+KRIKYHN YL N+AASIKED
Sbjct: 167 RSLMNYVKNRYNSPPIYITENGMDDSNSPFIALKDALKDTKRIKYHNDYLTNLAASIKED 346
Query: 185 GCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDYKDNLKRYPKNSVQWFKTLLSSS* 352
GCDVRGYFAWSLLDNWEWAAGY+SRFGLYFVDY D LKRYPK+SVQ FK+LLSSS*
Sbjct: 347 GCDVRGYFAWSLLDNWEWAAGYSSRFGLYFVDYNDKLKRYPKSSVQXFKSLLSSS* 514
Score = 139 bits (298), Expect = 2e-032
Identities = 66/120 (55%), Positives = 75/120 (62%)
Frame = +1 / +1
Query: 4 QEADELCQGAVQ*PNGLHH*KWDGRRQQPFHLHPGRPQGQQEDQVPQRLPQQRCCLHKXX 183
+E DEL Q VQ P LHH K DG +QQP H GR QG QEDQVPQRLP Q LHK
Sbjct: 166 EEPDELRQEQVQQPTDLHHRKRDG*QQQPLHCPQGRTQGHQEDQVPQRLPHQSGRLHKGG 345
Query: 184 XXXXXXXXXXXXXXQLGMGRRVHLQIRALLRGLQG*PQEVPQELGPVVQDPAELQLKASH 363
QLG+G RV +Q+RALLRGLQ QEVPQELG VQ+P +LQL+ S+
Sbjct: 346 RVRRARLLRVVSAGQLGVGGRVLVQVRALLRGLQRQAQEVPQELGAXVQEPPQLQLRLSY 525
Score = 103 bits (219), Expect(2) = 2e-036
Identities = 47/63 (74%), Positives = 49/63 (77%)
Frame = -2 / -3
Query: 351 QLELSRVLNHWTEFLGYXXXXXXXSTK*SPNLEVYPAAHSQLSSNDHAKYPRTSHPSSFM 172
QLEL R+LN TE LGY STK*SPNL+ YPAAHSQLSS DHAK PRTSHPSSFM
Sbjct: 513 QLELRRLLNXCTELLGYLLSLSL*STK*SPNLDEYPAAHSQLSSRDHAK*PRTSHPSSFM 334
Query: 171 EAA 163
EAA
Sbjct: 333 EAA 325
Score = 68.9 bits (144), Expect(2) = 2e-036
Identities = 24/35 (68%), Positives = 31/35 (88%)
Frame = -1 / -2
Query: 109 VLDGGERAVAVVHPIFSDVNRWAIVPLLDIVHQLP 5
VL+G E AVAV+HP+F DV+RWA+VP+LD+VHQ P
Sbjct: 271 VLEGNEGAVAVIHPVFGDVDRWAVVPVLDVVHQAP 167
>gb|CA023147.1|CA023147 HZ45F22r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ45F22
5-PRIME, mRNA sequence
Length = 450
Score = 166 bits (358), Expect = 9e-041
Identities = 57/97 (58%), Positives = 81/97 (83%)
Frame = +2 / +2
Query: 11 LMNYVKERYNSPTVYITENGMDDGNSPFTSIQDALKDSKRIKYHNGYLNNVAASIKEDGC 190
LM +VK++Y +P V+ITENGMDD NS F+ ++ L+D+KRI+YHN Y++N+ +I+++GC
Sbjct: 155 LMKHVKDKYGNPPVFITENGMDDANSRFSKLETVLQDNKRIQYHNDYMSNLLDAIRKEGC 334
Query: 191 DVRGYFAWSLLDNWEWAAGYTSRFGLYFVDYKDNLKR 301
++RGYF WSLLDNWEW +GYT RFGLY++DY +NL R
Sbjct: 335 NIRGYFVWSLLDNWEWNSGYTVRFGLYYIDYDNNLTR 445
>gb|BF254222.2|BF254222 HVSMEf0003F20f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0003F20f, mRNA sequence
Length = 556
Score = 155 bits (334), Expect = 2e-037
Identities = 59/81 (72%), Positives = 67/81 (82%)
Frame = +2 / +3
Query: 5 RKLMNYVKERYNSPTVYITENGMDDGNSPFTSIQDALKDSKRIKYHNGYLNNVAASIKED 184
++LMNYVK+RYN+PTVYITENGMDD NSPF ++ AL D+KRI YHN YL N+A SI ED
Sbjct: 303 KRLMNYVKDRYNTPTVYITENGMDDSNSPFIFLKKALHDTKRINYHNDYLTNLADSITED 482
Query: 185 GCDVRGYFAWSLLDNWEWAAG 247
CDV YFAWSLLDNWEW AG
Sbjct: 483 SCDVPRYFAWSLLDNWEWTAG 545
>gb|CB858437.1|CB858437 HI07A02w HI Hordeum vulgare subsp. vulgare cDNA clone HI07A02
3-PRIME, mRNA sequence
Length = 653
Score = 68.9 bits (144), Expect(3) = 2e-023
Identities = 23/35 (65%), Positives = 31/35 (88%)
Frame = +2 / -1
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
+DG +V GYFAWSLLDN+EW +GYTS+FG+ +VD+
Sbjct: 215 DDGANVVGYFAWSLLDNFEWLSGYTSKFGIVYVDF 111
Score = 42.8 bits (87), Expect(3) = 2e-023
Identities = 13/21 (61%), Positives = 20/21 (95%)
Frame = +2 / -1
Query: 14 MNYVKERYNSPTVYITENGMD 76
+NY++E+YN+PT+ I+ENGMD
Sbjct: 371 VNYIREKYNNPTIIISENGMD 309
Score = 35.0 bits (70), Expect(3) = 2e-023
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = +2 / -1
Query: 293 LKRYPKNSVQWFKTLLSSS 349
LKRYPK+S WFK +L +S
Sbjct: 104 LKRYPKDSAYWFKNMLQAS 48
>gb|BF623927.2|BF623927 HVSMEa0007M14f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0007M14f, mRNA sequence
Length = 725
Score = 70.3 bits (147), Expect(2) = 8e-023
Identities = 33/77 (42%), Positives = 44/77 (57%)
Frame = +2 / +1
Query: 167 ASIKEDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDYKDNLKRYPKNSVQWFKTLLSS 346
AS G DVRGYF WSLLD++EW +GY RF LY VD+K + ++ + + L SS
Sbjct: 409 ASAIRKGADVRGYFVWSLLDDFEWTSGYKDRFXLYHVDFKTQKRTPKLSAEWFRQFLKSS 588
Query: 347 S*RRHTRTVIGLIIYPT 397
R T+ L +Y T
Sbjct: 589 LLTRETQNGSQLQLYYT 639
Score = 56.5 bits (117), Expect(2) = 8e-023
Identities = 22/58 (37%), Positives = 38/58 (65%)
Frame = +2 / +1
Query: 8 KLMNYVKERYNSPTVYITENGMDDGNSPFTSIQDALKDSKRIKYHNGYLNNVAASIKE 181
K++ Y K+RYN+ YITENG ++ + +D D++RI Y +GYL +A++I++
Sbjct: 253 KVVMYYKQRYNNTATYITENGYAQASNSSMTAKDFTGDTERINYISGYLTYLASAIRK 426
>gb|BJ467618.1|BJ467618 BJ467618 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags29b06 3', mRNA sequence
Length = 709
Score = 70.7 bits (148), Expect(3) = 1e-022
Identities = 25/36 (69%), Positives = 30/36 (83%)
Frame = +2 / -2
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDYK 286
+DG V GYFAWSLLDN+EW GYT+RFG+ +VDYK
Sbjct: 312 DDGARVIGYFAWSLLDNFEWRLGYTARFGIVYVDYK 205
Score = 40.0 bits (81), Expect(3) = 1e-022
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +2 / -2
Query: 8 KLMNYVKERYNSPTVYITENGMD 76
K + YVKERY +PT+ + ENGMD
Sbjct: 474 KAVTYVKERYGNPTMILAENGMD 406
Score = 33.1 bits (66), Expect(3) = 1e-022
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = +2 / -2
Query: 293 LKRYPKNSVQWFKTLLSSS*R 355
LKRYPK+S WFK +L+ R
Sbjct: 201 LKRYPKDSALWFKNMLTEKKR 139
>gb|BU977487.1|BU977487 HA11I05r HA Hordeum vulgare subsp. vulgare cDNA clone HA11I05
5-PRIME, mRNA sequence
Length = 655
Score = 68.4 bits (143), Expect(3) = 1e-022
Identities = 27/58 (46%), Positives = 40/58 (68%)
Frame = +2 / +1
Query: 8 KLMNYVKERYNSPTVYITENGMDDGNSPFTSIQDALKDSKRIKYHNGYLNNVAASIKE 181
+LM Y K RYN+P VY+ ENG+D+GN+ I++AL+D RI YH +L + +IK+
Sbjct: 298 ELMLYAKRRYNNPAVYVMENGIDEGNNSSLPIKEALRDPARINYHYKHLLFLNLAIKQ 471
Score = 49.2 bits (101), Expect(3) = 1e-022
Identities = 14/30 (46%), Positives = 22/30 (73%)
Frame = +2 / +1
Query: 191 DVRGYFAWSLLDNWEWAAGYTSRFGLYFVD 280
+++GYF W+ +D +EW GY RFGL ++D
Sbjct: 478 NIKGYFTWTFMDCFEWGDGYKDRFGLIYID 567
Score = 25.8 bits (50), Expect(3) = 1e-022
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +2 / +1
Query: 284 KDNLKRYPKNSVQW 325
+ LKRYPK+S +W
Sbjct: 568 RSTLKRYPKDSSKW 609
>gb|BU968461.1|BU968461 HB07J14r BC Hordeum vulgare subsp. vulgare cDNA clone HB07J14
5-PRIME, mRNA sequence
Length = 523
Score = 68.4 bits (143), Expect(3) = 1e-022
Identities = 27/58 (46%), Positives = 40/58 (68%)
Frame = +2 / +2
Query: 8 KLMNYVKERYNSPTVYITENGMDDGNSPFTSIQDALKDSKRIKYHNGYLNNVAASIKE 181
+LM Y K RYN+P VY+ ENG+D+GN+ I++AL+D RI YH +L + +IK+
Sbjct: 80 ELMLYAKRRYNNPAVYVMENGIDEGNNSSLPIKEALRDPARINYHYKHLLFLNLAIKQ 253
Score = 49.2 bits (101), Expect(3) = 1e-022
Identities = 14/30 (46%), Positives = 22/30 (73%)
Frame = +2 / +2
Query: 191 DVRGYFAWSLLDNWEWAAGYTSRFGLYFVD 280
+++GYF W+ +D +EW GY RFGL ++D
Sbjct: 260 NIKGYFTWTFMDCFEWGDGYKDRFGLIYID 349
Score = 25.8 bits (50), Expect(3) = 1e-022
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +2 / +2
Query: 284 KDNLKRYPKNSVQW 325
+ LKRYPK+S +W
Sbjct: 350 RSTLKRYPKDSSKW 391
>gb|BU968389.1|BU968389 HB07F20r BC Hordeum vulgare subsp. vulgare cDNA clone HB07F20
5-PRIME, mRNA sequence
Length = 514
Score = 68.4 bits (143), Expect(3) = 1e-022
Identities = 27/58 (46%), Positives = 40/58 (68%)
Frame = +2 / +2
Query: 8 KLMNYVKERYNSPTVYITENGMDDGNSPFTSIQDALKDSKRIKYHNGYLNNVAASIKE 181
+LM Y K RYN+P VY+ ENG+D+GN+ I++AL+D RI YH +L + +IK+
Sbjct: 80 ELMLYAKRRYNNPAVYVMENGIDEGNNSSLPIKEALRDPARINYHYKHLLFLNLAIKQ 253
Score = 49.2 bits (101), Expect(3) = 1e-022
Identities = 14/30 (46%), Positives = 22/30 (73%)
Frame = +2 / +2
Query: 191 DVRGYFAWSLLDNWEWAAGYTSRFGLYFVD 280
+++GYF W+ +D +EW GY RFGL ++D
Sbjct: 260 NIKGYFTWTFMDCFEWGDGYKDRFGLIYID 349
Score = 25.8 bits (50), Expect(3) = 1e-022
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +2 / +2
Query: 284 KDNLKRYPKNSVQW 325
+ LKRYPK+S +W
Sbjct: 350 RSTLKRYPKDSSKW 391
>gb|BI951308.1|BI951308 HVSMEl0025H09f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0025H09f, mRNA sequence
Length = 565
Score = 65.2 bits (136), Expect(3) = 2e-022
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = +2 / +1
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G V GYFAWSLLDN+EW GYT+RFG+ +VD+
Sbjct: 280 DNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDF 384
Score = 40.5 bits (82), Expect(3) = 2e-022
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +2 / +1
Query: 8 KLMNYVKERYNSPTVYITENGMD 76
K + YVKERY +PT+ ++ENGMD
Sbjct: 118 KAVTYVKERYGNPTMILSENGMD 186
Score = 37.3 bits (75), Expect(3) = 2e-022
Identities = 14/27 (51%), Positives = 18/27 (66%)
Frame = +2 / +1
Query: 287 DNLKRYPKNSVQWFKTLLSSS*RRHTR 367
+ LKRYPK+S WFK +LS R + R
Sbjct: 385 NTLKRYPKDSALWFKNMLSEKKRSYDR 465
>gb|BE601818.2|BE601818 HVSMEh0099K11f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0099K11f, mRNA sequence
Length = 503
Score = 65.2 bits (136), Expect(3) = 5e-022
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = +2 / +1
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G V GYFAWSLLDN+EW GYT+RFG+ +VD+
Sbjct: 295 DNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDF 399
Score = 40.5 bits (82), Expect(3) = 5e-022
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +2 / +1
Query: 8 KLMNYVKERYNSPTVYITENGMD 76
K + YVKERY +PT+ ++ENGMD
Sbjct: 133 KAVTYVKERYGNPTMILSENGMD 201
Score = 35.9 bits (72), Expect(3) = 5e-022
Identities = 15/30 (50%), Positives = 18/30 (60%)
Frame = +2 / +1
Query: 287 DNLKRYPKNSVQWFKTLLSSS*RRHTRTVI 376
+ LKRYPK+S WFK +LS R R I
Sbjct: 400 NTLKRYPKDSALWFKNMLSEKKRELDRNRI 489
>gb|BQ659818.1|BQ659818 HF01M24w HF Hordeum vulgare subsp. vulgare cDNA clone HF01M24
3-PRIME, mRNA sequence
Length = 681
Score = 65.2 bits (136), Expect(3) = 9e-022
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = +2 / -1
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G V GYFAWSLLDN+EW GYT+RFG+ +VD+
Sbjct: 468 DNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDF 364
Score = 40.5 bits (82), Expect(3) = 9e-022
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +2 / -1
Query: 8 KLMNYVKERYNSPTVYITENGMD 76
K + YVKERY +PT+ ++ENGMD
Sbjct: 630 KAVTYVKERYGNPTMILSENGMD 562
Score = 35.0 bits (70), Expect(3) = 9e-022
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +2 / -1
Query: 287 DNLKRYPKNSVQWFKTLLSSS*R 355
+ LKRYPK+S WFK +LS R
Sbjct: 363 NTLKRYPKDSALWFKNMLSEKKR 295
>gb|BQ755347.1|BQ755347 EBed07_SQ002_J07_R endosperm, 28 DPA, no treatment, cv Optic,
EBed07 Hordeum vulgare subsp. vulgare cDNA clone
EBed07_SQ002_J07 5', mRNA sequence
Length = 642
Score = 65.2 bits (136), Expect(3) = 9e-022
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = +2 / +3
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G V GYFAWSLLDN+EW GYT+RFG+ +VD+
Sbjct: 387 DNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDF 491
Score = 40.5 bits (82), Expect(3) = 9e-022
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +2 / +3
Query: 8 KLMNYVKERYNSPTVYITENGMD 76
K + YVKERY +PT+ ++ENGMD
Sbjct: 225 KAVTYVKERYGNPTMILSENGMD 293
Score = 35.0 bits (70), Expect(3) = 9e-022
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +2 / +3
Query: 287 DNLKRYPKNSVQWFKTLLSSS*R 355
+ LKRYPK+S WFK +LS R
Sbjct: 492 NTLKRYPKDSALWFKNMLSEKKR 560
>gb|BE601804.3|BE601804 HVSMEh0099J17f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0099J17f, mRNA sequence
Length = 627
Score = 65.2 bits (136), Expect(3) = 9e-022
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = +2 / +2
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G V GYFAWSLLDN+EW GYT+RFG+ +VD+
Sbjct: 203 DNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDF 307
Score = 40.5 bits (82), Expect(3) = 9e-022
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +2 / +2
Query: 8 KLMNYVKERYNSPTVYITENGMD 76
K + YVKERY +PT+ ++ENGMD
Sbjct: 41 KAVTYVKERYGNPTMILSENGMD 109
Score = 35.0 bits (70), Expect(3) = 9e-022
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +2 / +2
Query: 287 DNLKRYPKNSVQWFKTLLSSS*R 355
+ LKRYPK+S WFK +LS R
Sbjct: 308 NTLKRYPKDSALWFKNMLSEKKR 376
>gb|BU986792.1|BU986792 HF12O16r HF Hordeum vulgare subsp. vulgare cDNA clone HF12O16
5-PRIME, mRNA sequence
Length = 615
Score = 65.2 bits (136), Expect(3) = 9e-022
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = +2 / +1
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G V GYFAWSLLDN+EW GYT+RFG+ +VD+
Sbjct: 397 DNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDF 501
Score = 40.5 bits (82), Expect(3) = 9e-022
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +2 / +1
Query: 8 KLMNYVKERYNSPTVYITENGMD 76
K + YVKERY +PT+ ++ENGMD
Sbjct: 235 KAVTYVKERYGNPTMILSENGMD 303
Score = 35.0 bits (70), Expect(3) = 9e-022
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +2 / +1
Query: 287 DNLKRYPKNSVQWFKTLLSSS*R 355
+ LKRYPK+S WFK +LS R
Sbjct: 502 NTLKRYPKDSALWFKNMLSEKKR 570
>gb|BQ466282.1|BQ466282 HT02B16r HT Hordeum vulgare subsp. vulgare cDNA clone HT02B16
5-PRIME, mRNA sequence
Length = 611
Score = 65.2 bits (136), Expect(3) = 9e-022
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = +2 / +1
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G V GYFAWSLLDN+EW GYT+RFG+ +VD+
Sbjct: 271 DNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDF 375
Score = 40.5 bits (82), Expect(3) = 9e-022
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +2 / +1
Query: 8 KLMNYVKERYNSPTVYITENGMD 76
K + YVKERY +PT+ ++ENGMD
Sbjct: 109 KAVTYVKERYGNPTMILSENGMD 177
Score = 35.0 bits (70), Expect(3) = 9e-022
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +2 / +1
Query: 287 DNLKRYPKNSVQWFKTLLSSS*R 355
+ LKRYPK+S WFK +LS R
Sbjct: 376 NTLKRYPKDSALWFKNMLSEKKR 444
>gb|BU984559.1|BU984559 HF04E16r HF Hordeum vulgare subsp. vulgare cDNA clone HF04E16
5-PRIME, mRNA sequence
Length = 590
Score = 65.2 bits (136), Expect(3) = 9e-022
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = +2 / +2
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G V GYFAWSLLDN+EW GYT+RFG+ +VD+
Sbjct: 353 DNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDF 457
Score = 40.5 bits (82), Expect(3) = 9e-022
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +2 / +2
Query: 8 KLMNYVKERYNSPTVYITENGMD 76
K + YVKERY +PT+ ++ENGMD
Sbjct: 191 KAVTYVKERYGNPTMILSENGMD 259
Score = 35.0 bits (70), Expect(3) = 9e-022
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +2 / +2
Query: 287 DNLKRYPKNSVQWFKTLLSSS*R 355
+ LKRYPK+S WFK +LS R
Sbjct: 458 NTLKRYPKDSALWFKNMLSEKKR 526
>gb|BQ472509.1|BQ472509 HB09F20T BC Hordeum vulgare subsp. vulgare cDNA clone HB09F20
5-PRIME, mRNA sequence
Length = 558
Score = 64.3 bits (134), Expect(3) = 2e-021
Identities = 26/58 (44%), Positives = 39/58 (67%)
Frame = +2 / +2
Query: 8 KLMNYVKERYNSPTVYITENGMDDGNSPFTSIQDALKDSKRIKYHNGYLNNVAASIKE 181
+LM Y K RYN+P VY+ ENG+D+ N+ I++AL+D RI YH +L + +IK+
Sbjct: 80 ELMLYAKRRYNNPAVYVMENGIDECNNSSLPIKEALRDPARINYHYKHLLFLNLAIKQ 253
Score = 49.2 bits (101), Expect(3) = 2e-021
Identities = 14/30 (46%), Positives = 22/30 (73%)
Frame = +2 / +2
Query: 191 DVRGYFAWSLLDNWEWAAGYTSRFGLYFVD 280
+++GYF W+ +D +EW GY RFGL ++D
Sbjct: 260 NIKGYFTWTFMDCFEWGDGYKDRFGLIYID 349
Score = 25.8 bits (50), Expect(3) = 2e-021
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +2 / +2
Query: 284 KDNLKRYPKNSVQW 325
+ LKRYPK+S +W
Sbjct: 350 RSTLKRYPKDSSKW 391
>gb|BE454083.2|BE454083 HVSMEh0080E16f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0080E16f, mRNA sequence
Length = 713
Score = 61.1 bits (127), Expect(3) = 4e-021
Identities = 21/33 (63%), Positives = 27/33 (81%)
Frame = +2 / +2
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFV 277
++G V GYFAWSLLDN+EW GYT+RFG+ +V
Sbjct: 221 DNGARVAGYFAWSLLDNFEWRLGYTARFGIVYV 319
Score = 40.5 bits (82), Expect(3) = 4e-021
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +2 / +2
Query: 8 KLMNYVKERYNSPTVYITENGMD 76
K + YVKERY +PT+ ++ENGMD
Sbjct: 59 KAVTYVKERYGNPTMILSENGMD 127
Score = 36.8 bits (74), Expect(3) = 4e-021
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = +2 / +2
Query: 281 YKDNLKRYPKNSVQWFKTLLSSS*R 355
Y + LKRYPK+S WFK +LS R
Sbjct: 320 YFNTLKRYPKDSALWFKNMLSEKKR 394
>gb|BI947748.1|BI947748 HVSMEl0006L16f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0006L16f, mRNA sequence
Length = 833
Score = 65.2 bits (136), Expect(3) = 6e-021
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = +2 / -3
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G V GYFAWSLLDN+EW GYT+RFG+ +VD+
Sbjct: 510 DNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDF 406
Score = 37.7 bits (76), Expect(3) = 6e-021
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = +2 / -3
Query: 8 KLMNYVKERYNSPTVYITENGMD 76
K + YVKERY +P + ++ENGMD
Sbjct: 672 KAVTYVKERYGNPPMILSENGMD 604
Score = 35.0 bits (70), Expect(3) = 6e-021
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +2 / -3
Query: 287 DNLKRYPKNSVQWFKTLLSSS*R 355
+ LKRYPK+S WFK +LS R
Sbjct: 405 NTLKRYPKDSALWFKNMLSEKKR 337
>gb|CV053661.1|CV053661 BNEL101g2 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL101g2 5' similar to
beta-glucosidase BGQ60 precursor - barley, mRNA sequence
Length = 617
Score = 64.3 bits (134), Expect(3) = 6e-021
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = +2 / +3
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G V GYFAWSLLDN+EW GYT+RFG+ +VD+
Sbjct: 159 DNGARVVGYFAWSLLDNFEWRLGYTARFGIVYVDF 263
Score = 38.6 bits (78), Expect(3) = 6e-021
Identities = 13/21 (61%), Positives = 18/21 (85%)
Frame = +2 / +3
Query: 14 MNYVKERYNSPTVYITENGMD 76
+ YVKERY +PT+ ++ENGMD
Sbjct: 3 VTYVKERYGNPTMILSENGMD 65
Score = 35.0 bits (70), Expect(3) = 6e-021
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = +2 / +3
Query: 287 DNLKRYPKNSVQWFKTLLSSS*R 355
+ LKRYPK+S WFK +LS R
Sbjct: 264 NTLKRYPKDSALWFKNMLSEKKR 332
>gb|BI949909.1|BI949909 HVSMEl0016O06f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0016O06f, mRNA sequence
Length = 445
Score = 65.2 bits (136), Expect(3) = 6e-021
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = +2 / +2
Query: 179 EDGCDVRGYFAWSLLDNWEWAAGYTSRFGLYFVDY 283
++G V GYFAWSLLDN+EW GYT+RFG+ +VD+
Sbjct: 167 DNGARVAGYFAWSLLDNFEWRLGYTARFGIVYVDF 271
Score = 40.5 bits (82), Expect(3) = 6e-021
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +2 / +2
Query: 8 KLMNYVKERYNSPTVYITENGMD 76
K + YVKERY +PT+ ++ENGMD
Sbjct: 5 KAVTYVKERYGNPTMILSENGMD 73
Score = 32.2 bits (64), Expect(3) = 6e-021
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +2 / +2
Query: 287 DNLKRYPKNSVQWFKTLLSSS*R 355
+ LK YPK+S WFK +LS R
Sbjct: 272 NTLKTYPKDSALWFKNMLSEKKR 340
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 256,771,243
Number of Sequences: 312970
Number of extensions: 3385058
Number of successful extensions: 42351
Number of sequences better than 1.0e-020: 28
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8144008128
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)