TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419405.2.4
(582 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ759881.1|BQ759881 EBpi05_SQ004_P16_R pistil, 8 DPA, no... 207 5e-053
gb|CB880652.1|CB880652 HM07E17w HM Hordeum vulgare subsp. v... 207 5e-053
gb|CB881234.1|CB881234 HM09C23w HM Hordeum vulgare subsp. v... 207 5e-053
gb|BU986289.1|BU986289 HF11E17r HF Hordeum vulgare subsp. v... 188 2e-047
gb|AV928430.1|AV928430 AV928430 K. Sato unpublished cDNA li... 175 7e-045
gb|BF260026.3|BF260026 HVSMEf0020O13f Hordeum vulgare seedl... 175 2e-043
gb|BQ467895.1|BQ467895 HR01E04r HR Hordeum vulgare subsp. v... 175 2e-043
gb|CK124171.1|CK124171 BES1824107e17 BES1824 Hordeum vulgar... 128 2e-042
gb|CK122346.1|CK122346 BES1824102p12 BES1824 Hordeum vulgar... 128 2e-042
gb|BE060246.3|BE060246 HVSMEg0011L08f Hordeum vulgare pre-a... 128 2e-042
gb|BQ459628.1|BQ459628 HA08J01r HA Hordeum vulgare subsp. v... 128 2e-042
gb|BU984183.1|BU984183 HF03D17r HF Hordeum vulgare subsp. v... 166 7e-041
gb|BM097179.2|BM097179 EBpi07_SQ002_K14_R pistil, 12 DPA, n... 164 5e-040
gb|BG416780.1|BG416780 HVSMEk0014H23f Hordeum vulgare testa... 128 2e-038
gb|BJ453165.1|BJ453165 BJ453165 K. Sato unpublished cDNA li... 147 1e-036
gb|CV055940.1|CV055940 BNEL12B5 Barley EST endosperm librar... 111 2e-036
gb|BI958420.1|BI958420 HVSMEn0014O10f Hordeum vulgare rachi... 116 3e-036
gb|BJ460714.1|BJ460714 BJ460714 K. Sato unpublished cDNA li... 144 6e-036
gb|CV058074.1|CV058074 BNEL34a7 Barley EST endosperm librar... 106 3e-035
gb|BQ470849.1|BQ470849 HX04D14r HX Hordeum vulgare subsp. v... 111 8e-035
gb|BQ459308.1|BQ459308 HA01I15r HA Hordeum vulgare subsp. v... 100 7e-033
gb|BF622288.3|BF622288 HVSMEa0002I16f Hordeum vulgare seedl... 103 2e-032
gb|BE421427.1|BE421427 HWM009.B12 ITEC HWM Barley Leaf Libr... 131 8e-032
gb|BI950777.1|BI950777 HVSMEl0023C15f Hordeum vulgare spike... 134 4e-031
gb|BI956772.1|BI956772 HVSMEn0005E06f Hordeum vulgare rachi... 126 1e-028
gb|AV917139.1|AV917139 AV917139 K. Sato unpublished cDNA li... 125 2e-028
gb|BM101447.2|BM101447 EBpi01_SQ003_O01_R pistil, 1 DPA, no... 125 2e-028
gb|CA017263.1|CA017263 HV14A15u HV Hordeum vulgare subsp. v... 125 2e-028
gb|AJ461923.1|AJ461923 AJ461923 S00002 Hordeum vulgare subs... 125 2e-028
gb|CA001233.1|CA001233 HS18N14u HS Hordeum vulgare subsp. v... 119 3e-028
gb|BE454367.2|BE454367 HVSMEh0093N18f Hordeum vulgare 5-45 ... 80 7e-028
gb|BQ661556.1|BQ661556 HM03P13u HM Hordeum vulgare subsp. v... 122 2e-027
gb|CB881625.1|CB881625 HM10G06w HM Hordeum vulgare subsp. v... 94 9e-027
gb|CB880524.1|CB880524 HM06N05w HM Hordeum vulgare subsp. v... 94 9e-027
gb|BQ661443.1|BQ661443 HM03I08u HM Hordeum vulgare subsp. v... 94 9e-027
gb|BI959138.1|BI959138 HVSMEn0018F10f Hordeum vulgare rachi... 79 2e-026
gb|CK123980.1|CK123980 BES1824106f16 BES1824 Hordeum vulgar... 94 2e-026
gb|AV918397.1|AV918397 AV918397 K. Sato unpublished cDNA li... 116 1e-025
gb|CB876346.1|CB876346 HX11A03w HX Hordeum vulgare subsp. v... 116 1e-025
gb|BJ463021.1|BJ463021 BJ463021 K. Sato unpublished cDNA li... 115 2e-025
gb|BU990646.1|BU990646 HF25K12r HF Hordeum vulgare subsp. v... 115 3e-025
gb|BM442090.2|BM442090 EBan01_SQ002_C16_R anther, yellow st... 114 4e-025
gb|AJ463236.1|AJ463236 AJ463236 S00002 Hordeum vulgare subs... 77 4e-025
gb|AV918336.1|AV918336 AV918336 K. Sato unpublished cDNA li... 114 5e-025
gb|CB881182.1|CB881182 HM09A07w HM Hordeum vulgare subsp. v... 112 1e-024
gb|CA001449.1|CA001449 HS17P08u HS Hordeum vulgare subsp. v... 106 2e-024
gb|CK122737.1|CK122737 BES1824105j22 BES1824 Hordeum vulgar... 70 3e-024
gb|BQ665573.1|BQ665573 HX04D14u HX Hordeum vulgare subsp. v... 111 3e-024
gb|BG299356.2|BG299356 HVSMEa0019J09f Hordeum vulgare seedl... 111 5e-024
gb|BQ658207.1|BQ658207 HA05E07u HA Hordeum vulgare subsp. v... 66 9e-024
gb|BF628930.2|BF628930 HVSMEb0009C23f Hordeum vulgare seedl... 109 1e-023
gb|BE196421.3|BE196421 HVSMEh0092D24f Hordeum vulgare 5-45 ... 108 3e-023
gb|BQ662029.1|BQ662029 HR01E04u HR Hordeum vulgare subsp. v... 100 1e-022
gb|BQ465138.1|BQ465138 HU02M06r HU Hordeum vulgare subsp. v... 64 4e-022
gb|BF256315.1|BF256315 HVSMEf0009N16f Hordeum vulgare seedl... 103 7e-022
gb|BQ767036.1|BQ767036 EBro08_SQ007_G24_R root, 3 week, dro... 103 1e-021
gb|BM097618.2|BM097618 EBem04_SQ002_D15_R embryo, 12 DPA, n... 63 1e-021
gb|BM098205.2|BM098205 EBpi03_SQ002_N14_R pistil, 4 DPA, no... 100 9e-021
gb|BU980984.1|BU980984 HA22E18r HA Hordeum vulgare subsp. v... 100 9e-021
>gb|BQ759881.1|BQ759881 EBpi05_SQ004_P16_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ004_P16 5', mRNA sequence
Length = 500
Score = 207 bits (446), Expect = 5e-053
Identities = 80/92 (86%), Positives = 89/92 (96%)
Frame = -2 / +2
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSL 402
E+GSNPVY AVLVE+EDGDGDVVQVDLMEANSG+WTPMRESWGSIWRLDSGHRL APFS+
Sbjct: 146 EQGSNPVYFAVLVEYEDGDGDVVQVDLMEANSGTWTPMRESWGSIWRLDSGHRLQAPFSM 325
Query: 401 RITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
RITNESGKTLVA++VIPANW P+T+YRSI+QY
Sbjct: 326 RITNESGKTLVADKVIPANWAPSTFYRSIVQY 421
Score = 146 bits (314), Expect = 8e-035
Identities = 64/92 (69%), Positives = 66/92 (71%)
Frame = +1 / -3
Query: 307 YWMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDP 486
YW MER* VLG QLAGMT ATSVLPDSLVMR EKGA RRWPESSRQ+DPHDSRIGVH P
Sbjct: 420 YWTMER*KVLGAQLAGMTLSATSVLPDSLVMRMEKGAWRRWPESSRQIDPHDSRIGVHVP 241
Query: 487 ELASMRXXXXXXXXXXXXXXXXARYTGLDPSS 582
ELASMR A+YTG DP S
Sbjct: 240 ELASMRSTCTTSPSPSSYSTSTAKYTGFDPCS 145
Score = 129 bits (277), Expect = 1e-029
Identities = 48/52 (92%), Positives = 48/52 (92%)
Frame = -3 / +3
Query: 508 WTSWRPTPGRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
WTSWRPTP RGRRCASRG SGGWTPATAS RPSPCASPTSPARRWWPTRSS
Sbjct: 219 WTSWRPTPVRGRRCASRGDQSGGWTPATASRRPSPCASPTSPARRWWPTRSS 374
Score = 123 bits (263), Expect(2) = 2e-029
Identities = 54/80 (67%), Positives = 56/80 (70%)
Frame = +3 / -2
Query: 342 PVGRDDLVGHQRLAGLVGDAQGEGRGEAVAGVQPPDGPPRLAHRRPRPGVGXXXXXXXXX 521
PVGRDDLVGHQRLAGLVGDA GEGR EAVAGVQPPD PRLAHRRPR GVG
Sbjct: 385 PVGRDDLVGHQRLAGLVGDAHGEGRLEAVAGVQPPD*SPRLAHRRPRTGVGLHEVHLHHV 206
Query: 522 XXXXXXXDEHRQVHRVGPLL 581
+H +VHRV PLL
Sbjct: 205 AVAVLVLHQHGEVHRVRPLL 146
Score = 25.4 bits (49), Expect(2) = 2e-029
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +2 / -1
Query: 308 TG*WNGSRCW 337
TG W+G RCW
Sbjct: 419 TGRWSGRRCW 390
>gb|CB880652.1|CB880652 HM07E17w HM Hordeum vulgare subsp. vulgare cDNA clone HM07E17
3-PRIME, mRNA sequence
Length = 562
Score = 207 bits (446), Expect = 5e-053
Identities = 80/92 (86%), Positives = 89/92 (96%)
Frame = -2 / -2
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSL 402
E+GSNPVY AVLVE+EDGDGDVVQVDLMEANSG+WTPMRESWGSIWRLDSGHRL APFS+
Sbjct: 555 EQGSNPVYFAVLVEYEDGDGDVVQVDLMEANSGTWTPMRESWGSIWRLDSGHRLQAPFSM 376
Query: 401 RITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
RITNESGKTLVA++VIPANW P+T+YRSI+QY
Sbjct: 375 RITNESGKTLVADKVIPANWAPSTFYRSIVQY 280
Score = 146 bits (314), Expect = 8e-035
Identities = 64/92 (69%), Positives = 66/92 (71%)
Frame = +1 / +2
Query: 307 YWMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDP 486
YW MER* VLG QLAGMT ATSVLPDSLVMR EKGA RRWPESSRQ+DPHDSRIGVH P
Sbjct: 281 YWTMER*KVLGAQLAGMTLSATSVLPDSLVMRMEKGAWRRWPESSRQIDPHDSRIGVHVP 460
Query: 487 ELASMRXXXXXXXXXXXXXXXXARYTGLDPSS 582
ELASMR A+YTG DP S
Sbjct: 461 ELASMRSTCTTSPSPSSYSTSTAKYTGFDPCS 556
Score = 129 bits (277), Expect = 1e-029
Identities = 48/52 (92%), Positives = 48/52 (92%)
Frame = -3 / -3
Query: 508 WTSWRPTPGRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
WTSWRPTP RGRRCASRG SGGWTPATAS RPSPCASPTSPARRWWPTRSS
Sbjct: 482 WTSWRPTPVRGRRCASRGDQSGGWTPATASRRPSPCASPTSPARRWWPTRSS 327
Score = 123 bits (263), Expect(2) = 2e-029
Identities = 54/80 (67%), Positives = 56/80 (70%)
Frame = +3 / +1
Query: 342 PVGRDDLVGHQRLAGLVGDAQGEGRGEAVAGVQPPDGPPRLAHRRPRPGVGXXXXXXXXX 521
PVGRDDLVGHQRLAGLVGDA GEGR EAVAGVQPPD PRLAHRRPR GVG
Sbjct: 316 PVGRDDLVGHQRLAGLVGDAHGEGRLEAVAGVQPPD*SPRLAHRRPRTGVGLHEVHLHHV 495
Query: 522 XXXXXXXDEHRQVHRVGPLL 581
+H +VHRV PLL
Sbjct: 496 AVAVLVLHQHGEVHRVRPLL 555
Score = 25.4 bits (49), Expect(2) = 2e-029
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +2 / +3
Query: 308 TG*WNGSRCW 337
TG W+G RCW
Sbjct: 282 TGRWSGRRCW 311
>gb|CB881234.1|CB881234 HM09C23w HM Hordeum vulgare subsp. vulgare cDNA clone HM09C23
3-PRIME, mRNA sequence
Length = 563
Score = 207 bits (446), Expect = 5e-053
Identities = 80/92 (86%), Positives = 89/92 (96%)
Frame = -2 / -2
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSL 402
E+GSNPVY AVLVE+EDGDGDVVQVDLMEANSG+WTPMRESWGSIWRLDSGHRL APFS+
Sbjct: 556 EQGSNPVYFAVLVEYEDGDGDVVQVDLMEANSGTWTPMRESWGSIWRLDSGHRLQAPFSM 377
Query: 401 RITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
RITNESGKTLVA++VIPANW P+T+YRSI+QY
Sbjct: 376 RITNESGKTLVADKVIPANWAPSTFYRSIVQY 281
Score = 146 bits (314), Expect = 8e-035
Identities = 64/92 (69%), Positives = 66/92 (71%)
Frame = +1 / +3
Query: 307 YWMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDP 486
YW MER* VLG QLAGMT ATSVLPDSLVMR EKGA RRWPESSRQ+DPHDSRIGVH P
Sbjct: 282 YWTMER*KVLGAQLAGMTLSATSVLPDSLVMRMEKGAWRRWPESSRQIDPHDSRIGVHVP 461
Query: 487 ELASMRXXXXXXXXXXXXXXXXARYTGLDPSS 582
ELASMR A+YTG DP S
Sbjct: 462 ELASMRSTCTTSPSPSSYSTSTAKYTGFDPCS 557
Score = 129 bits (277), Expect = 1e-029
Identities = 48/52 (92%), Positives = 48/52 (92%)
Frame = -3 / -3
Query: 508 WTSWRPTPGRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
WTSWRPTP RGRRCASRG SGGWTPATAS RPSPCASPTSPARRWWPTRSS
Sbjct: 483 WTSWRPTPVRGRRCASRGDQSGGWTPATASRRPSPCASPTSPARRWWPTRSS 328
Score = 123 bits (263), Expect(2) = 2e-029
Identities = 54/80 (67%), Positives = 56/80 (70%)
Frame = +3 / +2
Query: 342 PVGRDDLVGHQRLAGLVGDAQGEGRGEAVAGVQPPDGPPRLAHRRPRPGVGXXXXXXXXX 521
PVGRDDLVGHQRLAGLVGDA GEGR EAVAGVQPPD PRLAHRRPR GVG
Sbjct: 317 PVGRDDLVGHQRLAGLVGDAHGEGRLEAVAGVQPPD*SPRLAHRRPRTGVGLHEVHLHHV 496
Query: 522 XXXXXXXDEHRQVHRVGPLL 581
+H +VHRV PLL
Sbjct: 497 AVAVLVLHQHGEVHRVRPLL 556
Score = 25.4 bits (49), Expect(2) = 2e-029
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +2 / +1
Query: 308 TG*WNGSRCW 337
TG W+G RCW
Sbjct: 283 TGRWSGRRCW 312
>gb|BU986289.1|BU986289 HF11E17r HF Hordeum vulgare subsp. vulgare cDNA clone HF11E17
5-PRIME, mRNA sequence
Length = 538
Score = 188 bits (405), Expect = 2e-047
Identities = 73/83 (87%), Positives = 81/83 (97%)
Frame = -2 / +2
Query: 554 AVLVEFEDGDGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKT 375
AVLVE+EDGDGDVVQVDLMEANSG+WTPMRESWGSIWRLDSGHRL APFS+RITNESGKT
Sbjct: 11 AVLVEYEDGDGDVVQVDLMEANSGTWTPMRESWGSIWRLDSGHRLQAPFSMRITNESGKT 190
Query: 374 LVANQVIPANWVPNTYYRSIIQY 306
LVA++VIPANW P+T+YRSI+QY
Sbjct: 191 LVADKVIPANWAPSTFYRSIVQY 259
Score = 131 bits (281), Expect = 3e-030
Identities = 57/66 (86%), Positives = 58/66 (87%)
Frame = +1 / -2
Query: 307 YWMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDP 486
YW MER* VLG QLAGMT ATSVLPDSLVMR EKGA RRWPESSRQ+DPHDSRIGVH P
Sbjct: 258 YWTMER*KVLGAQLAGMTLSATSVLPDSLVMRMEKGAWRRWPESSRQIDPHDSRIGVHVP 79
Query: 487 ELASMR 504
ELASMR
Sbjct: 78 ELASMR 61
Score = 129 bits (277), Expect = 1e-029
Identities = 48/52 (92%), Positives = 48/52 (92%)
Frame = -3 / +3
Query: 508 WTSWRPTPGRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
WTSWRPTP RGRRCASRG SGGWTPATAS RPSPCASPTSPARRWWPTRSS
Sbjct: 57 WTSWRPTPVRGRRCASRGDQSGGWTPATASRRPSPCASPTSPARRWWPTRSS 212
Score = 110 bits (234), Expect(2) = 2e-025
Identities = 46/51 (90%), Positives = 46/51 (90%)
Frame = +3 / -1
Query: 342 PVGRDDLVGHQRLAGLVGDAQGEGRGEAVAGVQPPDGPPRLAHRRPRPGVG 494
PVGRDDLVGHQRLAGLVGDA GEGR EAVAGVQPPD PRLAHRRPR GVG
Sbjct: 223 PVGRDDLVGHQRLAGLVGDAHGEGRLEAVAGVQPPD*SPRLAHRRPRTGVG 71
Score = 25.4 bits (49), Expect(2) = 2e-025
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +2 / -3
Query: 308 TG*WNGSRCW 337
TG W+G RCW
Sbjct: 257 TGRWSGRRCW 228
>gb|AV928430.1|AV928430 AV928430 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basdg18 3', mRNA sequence
Length = 704
Score = 175 bits (376), Expect(2) = 7e-045
Identities = 68/91 (74%), Positives = 78/91 (85%)
Frame = -2 / -2
Query: 578 EGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLR 399
EGSN VYLAVL+E+ED DGDV+QVD+ EANSGSW MRESWGSIWR+DS HRL PFS+R
Sbjct: 616 EGSNAVYLAVLIEYEDMDGDVIQVDMKEANSGSWMAMRESWGSIWRMDSNHRLQGPFSMR 437
Query: 398 ITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
IT++SGK LVAN VIPANW PNT YRS +Q+
Sbjct: 436 ITSDSGKKLVANNVIPANWRPNTDYRSFVQF 344
Score = 126 bits (269), Expect(2) = 9e-031
Identities = 56/91 (61%), Positives = 60/91 (65%)
Frame = +1 / +3
Query: 310 WMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDPE 489
W ER* VLG QLAGMT LATS LP+SLVMR EKG RRW ES RQMDPHDSRI +HDPE
Sbjct: 348 WTKER*SVLGLQLAGMTLLATSFLPESLVMRMEKGPWRRWLESIRQMDPHDSRIAIHDPE 527
Query: 490 LASMRXXXXXXXXXXXXXXXXARYTGLDPSS 582
LAS ARYT L+PS+
Sbjct: 528 LASFMSTWITSPSMSSYSISTARYTALEPST 620
Score = 112 bits (240), Expect(2) = 3e-025
Identities = 48/79 (60%), Positives = 51/79 (64%)
Frame = +3 / +2
Query: 342 PVGRDDLVGHQRLAGLVGDAQGEGRGEAVAGVQPPDGPPRLAHRRPRPGVGXXXXXXXXX 521
PVGRDD+VGHQ LAG+ GDA GE EAV GV PPDG PRLAHR PRP VG
Sbjct: 380 PVGRDDIVGHQLLAGVAGDAHGERPLEAVVGVHPPDGSPRLAHRHPRPRVGLLHVHLDHV 559
Query: 522 XXXXXXXDEHRQVHRVGPL 578
D+HRQVHRVG L
Sbjct: 560 AVHVLVLDQHRQVHRVGAL 616
Score = 100 bits (214), Expect = 5e-021
Identities = 39/52 (75%), Positives = 40/52 (76%)
Frame = -3 / -3
Query: 508 WTSWRPTPGRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
WT RPT GRG RCASRG PSGGWTP TAS SPCASP +PAR WWPT SS
Sbjct: 546 WT*RRPTRGRGWRCASRGDPSGGWTPTTASRGLSPCASPATPARSWWPTMSS 391
Score = 28.6 bits (56), Expect(2) = 9e-031
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +1 / +3
Query: 40 IALVHYMHGQWAGKHLQINL 99
+ +VH QWAG+H+Q L
Sbjct: 45 LCMVHRTQSQWAGEHMQTML 104
Score = 26.7 bits (52), Expect(2) = 7e-045
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -2 / -2
Query: 95 LICRCFPAHCPCM*CT 48
++C C PAH C+ CT
Sbjct: 100 MVCMCSPAH*LCVRCT 53
Score = 23.1 bits (44), Expect(2) = 3e-025
Identities = 8/15 (53%), Positives = 9/15 (60%)
Frame = +2 / +1
Query: 47 WYITCMDNGRESICK 91
WY +GRES CK
Sbjct: 52 WYTVHRVSGRESTCK 96
>gb|BF260026.3|BF260026 HVSMEf0020O13f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020O13f, mRNA sequence
Length = 535
Score = 175 bits (376), Expect = 2e-043
Identities = 68/91 (74%), Positives = 78/91 (85%)
Frame = -2 / +1
Query: 578 EGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLR 399
EGSN VYLAVL+E+ED DGDV+QVD+ EANSGSW MRESWGSIWR+DS HRL PFS+R
Sbjct: 130 EGSNAVYLAVLIEYEDMDGDVIQVDMKEANSGSWMAMRESWGSIWRMDSNHRLQGPFSMR 309
Query: 398 ITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
IT++SGK LVAN VIPANW PNT YRS +Q+
Sbjct: 310 ITSDSGKKLVANNVIPANWRPNTDYRSFVQF 402
Score = 126 bits (269), Expect = 1e-028
Identities = 56/91 (61%), Positives = 60/91 (65%)
Frame = +1 / -3
Query: 310 WMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDPE 489
W ER* VLG QLAGMT LATS LP+SLVMR EKG RRW ES RQMDPHDSRI +HDPE
Sbjct: 398 WTKER*SVLGLQLAGMTLLATSFLPESLVMRMEKGPWRRWLESIRQMDPHDSRIAIHDPE 219
Query: 490 LASMRXXXXXXXXXXXXXXXXARYTGLDPSS 582
LAS ARYT L+PS+
Sbjct: 218 LASFMSTWITSPSMSSYSISTARYTALEPST 126
Score = 112 bits (240), Expect = 1e-024
Identities = 48/79 (60%), Positives = 51/79 (64%)
Frame = +3 / -2
Query: 342 PVGRDDLVGHQRLAGLVGDAQGEGRGEAVAGVQPPDGPPRLAHRRPRPGVGXXXXXXXXX 521
PVGRDD+VGHQ LAG+ GDA GE EAV GV PPDG PRLAHR PRP VG
Sbjct: 366 PVGRDDIVGHQLLAGVAGDAHGERPLEAVVGVHPPDGSPRLAHRHPRPRVGLLHVHLDHV 187
Query: 522 XXXXXXXDEHRQVHRVGPL 578
D+HRQVHRVG L
Sbjct: 186 AVHVLVLDQHRQVHRVGAL 130
Score = 100 bits (214), Expect = 5e-021
Identities = 39/52 (75%), Positives = 40/52 (76%)
Frame = -3 / +2
Query: 508 WTSWRPTPGRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
WT RPT GRG RCASRG PSGGWTP TAS SPCASP +PAR WWPT SS
Sbjct: 200 WT*RRPTRGRGWRCASRGDPSGGWTPTTASRGLSPCASPATPARSWWPTMSS 355
>gb|BQ467895.1|BQ467895 HR01E04r HR Hordeum vulgare subsp. vulgare cDNA clone HR01E04
5-PRIME, mRNA sequence
Length = 540
Score = 175 bits (376), Expect = 2e-043
Identities = 68/91 (74%), Positives = 78/91 (85%)
Frame = -2 / +2
Query: 578 EGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLR 399
EGSN VYLAVL+E+ED DGDV+QVD+ EANSGSW MRESWGSIWR+DS HRL PFS+R
Sbjct: 11 EGSNAVYLAVLIEYEDMDGDVIQVDMKEANSGSWMAMRESWGSIWRMDSNHRLQGPFSMR 190
Query: 398 ITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
IT++SGK LVAN VIPANW PNT YRS +Q+
Sbjct: 191 ITSDSGKKLVANNVIPANWRPNTDYRSFVQF 283
Score = 126 bits (269), Expect = 1e-028
Identities = 56/91 (61%), Positives = 60/91 (65%)
Frame = +1 / -1
Query: 310 WMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDPE 489
W ER* VLG QLAGMT LATS LP+SLVMR EKG RRW ES RQMDPHDSRI +HDPE
Sbjct: 279 WTKER*SVLGLQLAGMTLLATSFLPESLVMRMEKGPWRRWLESIRQMDPHDSRIAIHDPE 100
Query: 490 LASMRXXXXXXXXXXXXXXXXARYTGLDPSS 582
LAS ARYT L+PS+
Sbjct: 99 LASFMSTWITSPSMSSYSISTARYTALEPST 7
Score = 112 bits (240), Expect = 1e-024
Identities = 48/79 (60%), Positives = 51/79 (64%)
Frame = +3 / -3
Query: 342 PVGRDDLVGHQRLAGLVGDAQGEGRGEAVAGVQPPDGPPRLAHRRPRPGVGXXXXXXXXX 521
PVGRDD+VGHQ LAG+ GDA GE EAV GV PPDG PRLAHR PRP VG
Sbjct: 247 PVGRDDIVGHQLLAGVAGDAHGERPLEAVVGVHPPDGSPRLAHRHPRPRVGLLHVHLDHV 68
Query: 522 XXXXXXXDEHRQVHRVGPL 578
D+HRQVHRVG L
Sbjct: 67 AVHVLVLDQHRQVHRVGAL 11
Score = 100 bits (214), Expect = 5e-021
Identities = 39/52 (75%), Positives = 40/52 (76%)
Frame = -3 / +3
Query: 508 WTSWRPTPGRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
WT RPT GRG RCASRG PSGGWTP TAS SPCASP +PAR WWPT SS
Sbjct: 81 WT*RRPTRGRGWRCASRGDPSGGWTPTTASRGLSPCASPATPARSWWPTMSS 236
>gb|CK124171.1|CK124171 BES1824107e17 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010E177 5-PRIME, mRNA sequence
Length = 751
Score = 128 bits (275), Expect(2) = 2e-042
Identities = 48/60 (80%), Positives = 53/60 (88%)
Frame = -2 / +1
Query: 485 GSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
G WT MRESWGS+WRLDS HRL APFS+RI NESGKTLVAN+VIPANW PNT+YRS +QY
Sbjct: 169 GKWTRMRESWGSVWRLDSNHRLQAPFSIRIRNESGKTLVANKVIPANWRPNTFYRSFVQY 348
Score = 63.4 bits (132), Expect(2) = 2e-042
Identities = 26/30 (86%), Positives = 28/30 (93%)
Frame = -2 / +1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
EE SNPVY AVLVE+EDGDGDVVQVDLME+
Sbjct: 64 EEYSNPVYFAVLVEYEDGDGDVVQVDLMES 153
>gb|CK122346.1|CK122346 BES1824102p12 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P122 5-PRIME, mRNA sequence
Length = 717
Score = 128 bits (275), Expect(2) = 2e-042
Identities = 48/60 (80%), Positives = 53/60 (88%)
Frame = -2 / +1
Query: 485 GSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
G WT MRESWGS+WRLDS HRL APFS+RI NESGKTLVAN+VIPANW PNT+YRS +QY
Sbjct: 406 GKWTRMRESWGSVWRLDSNHRLQAPFSIRIRNESGKTLVANKVIPANWRPNTFYRSFVQY 585
Score = 63.4 bits (132), Expect(2) = 2e-042
Identities = 26/30 (86%), Positives = 28/30 (93%)
Frame = -2 / +1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
EE SNPVY AVLVE+EDGDGDVVQVDLME+
Sbjct: 301 EEYSNPVYFAVLVEYEDGDGDVVQVDLMES 390
>gb|BE060246.3|BE060246 HVSMEg0011L08f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011L08f, mRNA sequence
Length = 544
Score = 128 bits (275), Expect(2) = 2e-042
Identities = 48/60 (80%), Positives = 53/60 (88%)
Frame = -2 / +2
Query: 485 GSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
G WT MRESWGS+WRLDS HRL APFS+RI NESGKTLVAN+VIPANW PNT+YRS +QY
Sbjct: 278 GKWTRMRESWGSVWRLDSNHRLQAPFSIRIRNESGKTLVANKVIPANWRPNTFYRSFVQY 457
Score = 63.4 bits (132), Expect(2) = 2e-042
Identities = 26/30 (86%), Positives = 28/30 (93%)
Frame = -2 / +2
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
EE SNPVY AVLVE+EDGDGDVVQVDLME+
Sbjct: 173 EEYSNPVYFAVLVEYEDGDGDVVQVDLMES 262
>gb|BQ459628.1|BQ459628 HA08J01r HA Hordeum vulgare subsp. vulgare cDNA clone HA08J01
5-PRIME, mRNA sequence
Length = 470
Score = 128 bits (275), Expect(2) = 2e-042
Identities = 48/60 (80%), Positives = 53/60 (88%)
Frame = -2 / +1
Query: 485 GSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
G WT MRESWGS+WRLDS HRL APFS+RI NESGKTLVAN+VIPANW PNT+YRS +QY
Sbjct: 238 GKWTRMRESWGSVWRLDSNHRLQAPFSIRIRNESGKTLVANKVIPANWRPNTFYRSFVQY 417
Score = 63.4 bits (132), Expect(2) = 2e-042
Identities = 26/30 (86%), Positives = 28/30 (93%)
Frame = -2 / +1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
EE SNPVY AVLVE+EDGDGDVVQVDLME+
Sbjct: 133 EEYSNPVYFAVLVEYEDGDGDVVQVDLMES 222
>gb|BU984183.1|BU984183 HF03D17r HF Hordeum vulgare subsp. vulgare cDNA clone HF03D17
5-PRIME, mRNA sequence
Length = 386
Score = 166 bits (358), Expect = 7e-041
Identities = 64/73 (87%), Positives = 71/73 (97%)
Frame = -2 / +2
Query: 524 GDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPAN 345
GDVVQVDLMEANSG+WTPMRESWGSIWRLDSGHRL APFS+RITNESGKTLVA++VIPAN
Sbjct: 2 GDVVQVDLMEANSGTWTPMRESWGSIWRLDSGHRLQAPFSMRITNESGKTLVADKVIPAN 181
Query: 344 WVPNTYYRSIIQY 306
W P+T+YRSI+QY
Sbjct: 182 WAPSTFYRSIVQY 220
Score = 131 bits (281), Expect = 3e-030
Identities = 57/66 (86%), Positives = 58/66 (87%)
Frame = +1 / -3
Query: 307 YWMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDP 486
YW MER* VLG QLAGMT ATSVLPDSLVMR EKGA RRWPESSRQ+DPHDSRIGVH P
Sbjct: 219 YWTMER*KVLGAQLAGMTLSATSVLPDSLVMRMEKGAWRRWPESSRQIDPHDSRIGVHVP 40
Query: 487 ELASMR 504
ELASMR
Sbjct: 39 ELASMR 22
Score = 129 bits (277), Expect = 1e-029
Identities = 48/52 (92%), Positives = 48/52 (92%)
Frame = -3 / +3
Query: 508 WTSWRPTPGRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
WTSWRPTP RGRRCASRG SGGWTPATAS RPSPCASPTSPARRWWPTRSS
Sbjct: 18 WTSWRPTPVRGRRCASRGDQSGGWTPATASRRPSPCASPTSPARRWWPTRSS 173
Score = 110 bits (234), Expect(2) = 1e-025
Identities = 46/51 (90%), Positives = 46/51 (90%)
Frame = +3 / -2
Query: 342 PVGRDDLVGHQRLAGLVGDAQGEGRGEAVAGVQPPDGPPRLAHRRPRPGVG 494
PVGRDDLVGHQRLAGLVGDA GEGR EAVAGVQPPD PRLAHRRPR GVG
Sbjct: 184 PVGRDDLVGHQRLAGLVGDAHGEGRLEAVAGVQPPD*SPRLAHRRPRTGVG 32
Score = 25.4 bits (49), Expect(2) = 1e-025
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +2 / -1
Query: 308 TG*WNGSRCW 337
TG W+G RCW
Sbjct: 218 TGRWSGRRCW 189
>gb|BM097179.2|BM097179 EBpi07_SQ002_K14_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ002_K14 5', mRNA sequence
Length = 382
Score = 164 bits (352), Expect = 5e-040
Identities = 63/72 (87%), Positives = 70/72 (97%)
Frame = -2 / +1
Query: 521 DVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANW 342
DVVQVDLMEANSG+WTPMRESWGSIWRLDSGHRL APFS+RITNESGKTLVA++VIPANW
Sbjct: 1 DVVQVDLMEANSGTWTPMRESWGSIWRLDSGHRLQAPFSMRITNESGKTLVADKVIPANW 180
Query: 341 VPNTYYRSIIQY 306
P+T+YRSI+QY
Sbjct: 181 APSTFYRSIVQY 216
Score = 131 bits (281), Expect = 3e-030
Identities = 57/66 (86%), Positives = 58/66 (87%)
Frame = +1 / -3
Query: 307 YWMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDP 486
YW MER* VLG QLAGMT ATSVLPDSLVMR EKGA RRWPESSRQ+DPHDSRIGVH P
Sbjct: 215 YWTMER*KVLGAQLAGMTLSATSVLPDSLVMRMEKGAWRRWPESSRQIDPHDSRIGVHVP 36
Query: 487 ELASMR 504
ELASMR
Sbjct: 35 ELASMR 18
Score = 129 bits (277), Expect = 1e-029
Identities = 48/52 (92%), Positives = 48/52 (92%)
Frame = -3 / +2
Query: 508 WTSWRPTPGRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
WTSWRPTP RGRRCASRG SGGWTPATAS RPSPCASPTSPARRWWPTRSS
Sbjct: 14 WTSWRPTPVRGRRCASRGDQSGGWTPATASRRPSPCASPTSPARRWWPTRSS 169
Score = 110 bits (234), Expect(2) = 1e-025
Identities = 46/51 (90%), Positives = 46/51 (90%)
Frame = +3 / -2
Query: 342 PVGRDDLVGHQRLAGLVGDAQGEGRGEAVAGVQPPDGPPRLAHRRPRPGVG 494
PVGRDDLVGHQRLAGLVGDA GEGR EAVAGVQPPD PRLAHRRPR GVG
Sbjct: 180 PVGRDDLVGHQRLAGLVGDAHGEGRLEAVAGVQPPD*SPRLAHRRPRTGVG 28
Score = 25.4 bits (49), Expect(2) = 1e-025
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = +2 / -1
Query: 308 TG*WNGSRCW 337
TG W+G RCW
Sbjct: 214 TGRWSGRRCW 185
>gb|BG416780.1|BG416780 HVSMEk0014H23f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0014H23f, mRNA sequence
Length = 696
Score = 128 bits (275), Expect(2) = 2e-038
Identities = 48/60 (80%), Positives = 53/60 (88%)
Frame = -2 / +1
Query: 485 GSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
G WT MRESWGS+WRLDS HRL APFS+RI NESGKTLVAN+VIPANW PNT+YRS +QY
Sbjct: 88 GKWTRMRESWGSVWRLDSNHRLQAPFSIRIRNESGKTLVANKVIPANWRPNTFYRSFVQY 267
Score = 50.1 bits (103), Expect(2) = 2e-038
Identities = 20/24 (83%), Positives = 22/24 (91%)
Frame = -2 / +1
Query: 563 VYLAVLVEFEDGDGDVVQVDLMEA 492
VY VLVE+EDGDGDVVQVDLME+
Sbjct: 1 VYFLVLVEYEDGDGDVVQVDLMES 72
>gb|BJ453165.1|BJ453165 BJ453165 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41p01 5', mRNA sequence
Length = 608
Score = 147 bits (316), Expect(2) = 1e-036
Identities = 56/75 (74%), Positives = 64/75 (85%)
Frame = -2 / +1
Query: 530 GDGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIP 351
GDGDV+QVD+ EANSGSW MRESWGSIWR+DS HRL PFS+RIT++SGK LVAN VIP
Sbjct: 19 GDGDVIQVDMKEANSGSWMAMRESWGSIWRMDSNHRLQGPFSMRITSDSGKKLVANNVIP 198
Query: 350 ANWVPNTYYRSIIQY 306
ANW PNT YRS +Q+
Sbjct: 199 ANWRPNTDYRSFVQF 243
Score = 112 bits (239), Expect(2) = 9e-027
Identities = 49/63 (77%), Positives = 51/63 (80%)
Frame = +1 / -1
Query: 310 WMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDPE 489
W ER* VLG QLAGMT LATS LP+SLVMR EKG RRW ES RQMDPHDSRI +HDPE
Sbjct: 239 WTKER*SVLGLQLAGMTLLATSFLPESLVMRMEKGPWRRWLESIRQMDPHDSRIAIHDPE 60
Query: 490 LAS 498
LAS
Sbjct: 59 LAS 51
Score = 100 bits (214), Expect = 5e-021
Identities = 39/52 (75%), Positives = 40/52 (76%)
Frame = -3 / +2
Query: 508 WTSWRPTPGRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
WT RPT GRG RCASRG PSGGWTP TAS SPCASP +PAR WWPT SS
Sbjct: 41 WT*RRPTRGRGWRCASRGDPSGGWTPTTASRGLSPCASPATPARSWWPTMSS 196
Score = 28.6 bits (56), Expect(2) = 9e-027
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +1 / -1
Query: 40 IALVHYMHGQWAGKHLQINL 99
+ +VH QWAG+H+Q L
Sbjct: 542 LCMVHRTQSQWAGEHMQTML 483
Score = 26.7 bits (52), Expect(2) = 1e-036
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -2 / +1
Query: 95 LICRCFPAHCPCM*CT 48
++C C PAH C+ CT
Sbjct: 487 MVCMCSPAH*LCVRCT 534
>gb|CV055940.1|CV055940 BNEL12B5 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL12B5 5' similar to Oryza sativa
beta-expansin, mRNA sequence
Length = 614
Score = 111 bits (236), Expect(2) = 2e-036
Identities = 39/61 (63%), Positives = 50/61 (81%)
Frame = -2 / -2
Query: 488 SGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQ 309
+G W PM+ESWGSIWR+D+ H + PFSLR+TNESGKTLVA+QVIP +W PN Y S++Q
Sbjct: 358 TGQWVPMKESWGSIWRMDAHHPMHGPFSLRVTNESGKTLVADQVIPGDWKPNKTYSSLVQ 179
Query: 308 Y 306
+
Sbjct: 178 F 176
Score = 61.1 bits (127), Expect(2) = 2e-036
Identities = 22/34 (64%), Positives = 30/34 (88%)
Frame = -2 / -2
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGS 480
EEGSNP Y+A+LVE+E+GDGDV Q+D+ME+ G+
Sbjct: 484 EEGSNPFYMAILVEYENGDGDVKQLDIMESRPGA 383
>gb|BI958420.1|BI958420 HVSMEn0014O10f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0014O10f, mRNA sequence
Length = 689
Score = 116 bits (249), Expect(2) = 3e-036
Identities = 45/60 (75%), Positives = 50/60 (83%)
Frame = -2 / -2
Query: 485 GSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
G T MRESWGS+W DS HRL APFS+RI NESGKTLVAN+VIPANW PNT+YRS +QY
Sbjct: 580 GK*TRMRESWGSVWXXDSNHRLQAPFSIRIRNESGKTLVANKVIPANWRPNTFYRSFVQY 401
Score = 54.2 bits (112), Expect(2) = 3e-036
Identities = 22/29 (75%), Positives = 26/29 (89%)
Frame = -2 / -2
Query: 578 EGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
E SNPVY A LVE+E+GDGDVVQVDLM++
Sbjct: 682 EYSNPVYFAGLVEYEEGDGDVVQVDLMKS 596
>gb|BJ460714.1|BJ460714 BJ460714 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41p01 3', mRNA sequence
Length = 573
Score = 144 bits (310), Expect(2) = 6e-036
Identities = 55/74 (74%), Positives = 63/74 (85%)
Frame = -2 / -3
Query: 527 DGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPA 348
DGDV+QVD+ EANSGSW MRESWGSIWR+DS HRL PFS+RIT++SGK LVAN VIPA
Sbjct: 571 DGDVIQVDMKEANSGSWMAMRESWGSIWRMDSNHRLQGPFSMRITSDSGKKLVANNVIPA 392
Query: 347 NWVPNTYYRSIIQY 306
NW PNT YRS +Q+
Sbjct: 391 NWRPNTDYRSFVQF 350
Score = 112 bits (239), Expect(2) = 9e-027
Identities = 49/63 (77%), Positives = 51/63 (80%)
Frame = +1 / +3
Query: 310 WMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDPE 489
W ER* VLG QLAGMT LATS LP+SLVMR EKG RRW ES RQMDPHDSRI +HDPE
Sbjct: 354 WTKER*SVLGLQLAGMTLLATSFLPESLVMRMEKGPWRRWLESIRQMDPHDSRIAIHDPE 533
Query: 490 LAS 498
LAS
Sbjct: 534 LAS 542
Score = 100 bits (214), Expect = 5e-021
Identities = 39/52 (75%), Positives = 40/52 (76%)
Frame = -3 / -1
Query: 508 WTSWRPTPGRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
WT RPT GRG RCASRG PSGGWTP TAS SPCASP +PAR WWPT SS
Sbjct: 552 WT*RRPTRGRGWRCASRGDPSGGWTPTTASRGLSPCASPATPARSWWPTMSS 397
Score = 28.6 bits (56), Expect(2) = 9e-027
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +1 / +3
Query: 40 IALVHYMHGQWAGKHLQINL 99
+ +VH QWAG+H+Q L
Sbjct: 51 LCMVHRTQSQWAGEHMQTML 110
Score = 26.7 bits (52), Expect(2) = 6e-036
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -2 / -3
Query: 95 LICRCFPAHCPCM*CT 48
++C C PAH C+ CT
Sbjct: 106 MVCMCSPAH*LCVRCT 59
>gb|CV058074.1|CV058074 BNEL34a7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL34a7 5' similar to Oryza sativa
beta-expansin, mRNA sequence
Length = 630
Score = 106 bits (227), Expect(2) = 3e-035
Identities = 38/61 (62%), Positives = 49/61 (80%)
Frame = -2 / +3
Query: 488 SGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQ 309
+G W PM+ESWGSIWR+D+ H + PFSLR+TNESGKTLVA+QVI +W PN Y S++Q
Sbjct: 270 TGQWVPMKESWGSIWRMDAHHPMHGPFSLRVTNESGKTLVADQVIXGDWKPNKTYSSLVQ 449
Query: 308 Y 306
+
Sbjct: 450 F 452
Score = 61.1 bits (127), Expect(2) = 3e-035
Identities = 22/34 (64%), Positives = 30/34 (88%)
Frame = -2 / +3
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGS 480
EEGSNP Y+A+LVE+E+GDGDV Q+D+ME+ G+
Sbjct: 144 EEGSNPFYMAILVEYENGDGDVKQLDIMESRPGA 245
>gb|BQ470849.1|BQ470849 HX04D14r HX Hordeum vulgare subsp. vulgare cDNA clone HX04D14
5-PRIME, mRNA sequence
Length = 606
Score = 111 bits (237), Expect(2) = 8e-035
Identities = 43/63 (68%), Positives = 50/63 (79%)
Frame = -2 / +2
Query: 494 ANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSI 315
A +G W PM ESWGSIWR+D+ + PFSLRITNESGKTLVA+QVIPA+W PN Y SI
Sbjct: 173 APTGEWVPMTESWGSIWRMDTRRPMQGPFSLRITNESGKTLVADQVIPADWEPNEIYSSI 352
Query: 314 IQY 306
IQ+
Sbjct: 353 IQF 361
Score = 55.1 bits (114), Expect(2) = 8e-035
Identities = 20/30 (66%), Positives = 27/30 (90%)
Frame = -2 / +2
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
+ GSNP YLA+LVE+E+GDGDV QVD+M++
Sbjct: 50 QHGSNPYYLAILVEYENGDGDVDQVDMMQS 139
>gb|BQ459308.1|BQ459308 HA01I15r HA Hordeum vulgare subsp. vulgare cDNA clone HA01I15
5-PRIME, mRNA sequence
Length = 396
Score = 100 bits (213), Expect(2) = 7e-033
Identities = 36/57 (63%), Positives = 45/57 (78%)
Frame = -2 / +2
Query: 488 SGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRS 318
+G W PM+ESWGSIWR+D+ H + PF LR+TNESGKTLVA++VIP +W PN Y S
Sbjct: 224 TGEWVPMKESWGSIWRMDAHHPMRGPFWLRVTNESGKTLVADKVIPGDWKPNKTYSS 394
Score = 59.3 bits (123), Expect(2) = 7e-033
Identities = 21/33 (63%), Positives = 29/33 (87%)
Frame = -2 / +2
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSG 483
EEGSNP Y+A+LVE+++GDGDV Q+D+ME+ G
Sbjct: 98 EEGSNPFYMAILVEYDNGDGDVKQLDIMESRPG 196
>gb|BF622288.3|BF622288 HVSMEa0002I16f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0002I16f, mRNA sequence
Length = 851
Score = 103 bits (219), Expect(2) = 2e-032
Identities = 40/62 (64%), Positives = 46/62 (74%)
Frame = -2 / +2
Query: 494 ANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSI 315
A +G W PM ESWGSIWR+D+ + PFSLRITNESGKTLVA+QV P +W PN Y S
Sbjct: 566 APTGEWVPMTESWGSIWRMDTRRPMQGPFSLRITNESGKTLVADQVNPGDWEPNEIYSSF 745
Query: 314 IQ 309
IQ
Sbjct: 746 IQ 751
Score = 55.1 bits (114), Expect(2) = 2e-032
Identities = 20/30 (66%), Positives = 27/30 (90%)
Frame = -2 / +2
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
+ GSNP YLA+LVE+E+GDGDV QVD+M++
Sbjct: 443 QHGSNPYYLAILVEYENGDGDVDQVDMMQS 532
>gb|BE421427.1|BE421427 HWM009.B12 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM009.B12, mRNA sequence
Length = 808
Score = 131 bits (281), Expect(2) = 8e-032
Identities = 50/69 (72%), Positives = 58/69 (84%)
Frame = -2 / +2
Query: 512 QVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPN 333
+VD+ EANSGSW MRESWGSIWR+DS HRL PFS+RIT++SGK LVAN VIPANW PN
Sbjct: 119 RVDMKEANSGSWMAMRESWGSIWRMDSNHRLQGPFSMRITSDSGKKLVANNVIPANWRPN 298
Query: 332 TYYRSIIQY 306
T YRS +Q+
Sbjct: 299 TDYRSFVQF 325
Score = 112 bits (239), Expect(2) = 1e-026
Identities = 49/63 (77%), Positives = 51/63 (80%)
Frame = +1 / -2
Query: 310 WMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDPE 489
W ER* VLG QLAGMT LATS LP+SLVMR EKG RRW ES RQMDPHDSRI +HDPE
Sbjct: 321 WTKER*SVLGLQLAGMTLLATSFLPESLVMRMEKGPWRRWLESIRQMDPHDSRIAIHDPE 142
Query: 490 LAS 498
LAS
Sbjct: 141 LAS 133
Score = 100 bits (214), Expect = 5e-021
Identities = 39/52 (75%), Positives = 40/52 (76%)
Frame = -3 / +3
Query: 508 WTSWRPTPGRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
WT RPT GRG RCASRG PSGGWTP TAS SPCASP +PAR WWPT SS
Sbjct: 123 WT*RRPTRGRGWRCASRGDPSGGWTPTTASRGLSPCASPATPARSWWPTMSS 278
Score = 28.6 bits (56), Expect(2) = 1e-026
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +1 / -2
Query: 40 IALVHYMHGQWAGKHLQINL 99
+ +VH QWAG+H+Q L
Sbjct: 624 LCMVHRTQSQWAGEHMQTML 565
Score = 26.7 bits (52), Expect(2) = 8e-032
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -2 / +2
Query: 95 LICRCFPAHCPCM*CT 48
++C C PAH C+ CT
Sbjct: 569 MVCMCSPAH*LCVRCT 616
>gb|BI950777.1|BI950777 HVSMEl0023C15f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023C15f, mRNA sequence
Length = 986
Score = 134 bits (287), Expect = 4e-031
Identities = 50/92 (54%), Positives = 68/92 (73%)
Frame = -2 / +3
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSL 402
++GSNP Y L+EFED DGD+ V L EA SG+WT M + WG++WRL++G+RL APFSL
Sbjct: 309 DQGSNPFYFKTLIEFEDDDGDLKAVALKEAGSGAWTQMTQDWGALWRLNNGNRLRAPFSL 488
Query: 401 RITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
++T++SG+ LV N VI ANW YRS++ Y
Sbjct: 489 QLTSDSGRKLVINNVILANWNAGATYRSLVNY 584
>gb|BI956772.1|BI956772 HVSMEn0005E06f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005E06f, mRNA sequence
Length = 796
Score = 126 bits (269), Expect = 1e-028
Identities = 52/77 (67%), Positives = 58/77 (75%)
Frame = -2 / +1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSL 402
E+GSNPVY AVLVE+ DGDGD+VQVDLMEANS +WTPMRESWGSI RLDSGH P S
Sbjct: 466 EQGSNPVYFAVLVEYXDGDGDMVQVDLMEANSCTWTPMRESWGSILRLDSGHGWRPPLSF 645
Query: 401 RITNESGKTLVANQVIP 351
+ NE G+ L Q P
Sbjct: 646 ALXNEGGRELGGRQGDP 696
>gb|AV917139.1|AV917139 AV917139 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20o17 5', mRNA sequence
Length = 590
Score = 125 bits (268), Expect = 2e-028
Identities = 49/72 (68%), Positives = 55/72 (76%)
Frame = -2 / +1
Query: 521 DVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANW 342
D+++ G WT MRESWGSIWRLDS HRL APFS+RI NESGKTLVA VIP NW
Sbjct: 298 DLMESGRGRRGGGRWTRMRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIPKNW 477
Query: 341 VPNTYYRSIIQY 306
PNT+YRSI+QY
Sbjct: 478 RPNTFYRSIVQY 513
Score = 100 bits (214), Expect(2) = 1e-022
Identities = 45/60 (75%), Positives = 45/60 (75%)
Frame = +1 / -1
Query: 307 YWMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDP 486
YW MER* VLG Q GMT ATSVLPDSL MR E GA RRW ES RQMDPHDSRI VH P
Sbjct: 512 YWTMER*KVLGLQFLGMTLRATSVLPDSLRMRMENGAWRRWLESRRQMDPHDSRIRVHRP 333
Score = 64.3 bits (134), Expect(2) = 3e-022
Identities = 25/33 (75%), Positives = 30/33 (90%)
Frame = -2 / +1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSG 483
EEGSN VY+A+LVE+E+GDGDVVQVDLME+ G
Sbjct: 223 EEGSNAVYMAILVEYENGDGDVVQVDLMESGRG 321
Score = 60.2 bits (125), Expect(2) = 3e-022
Identities = 28/44 (63%), Positives = 28/44 (63%)
Frame = -3 / +2
Query: 484 GRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
G GR CASRG PSG TP S R SP AS TSPARR P SS
Sbjct: 335 GGGRGCASRGDPSGASTPTIVSRRHSPSASATSPARRSSPATSS 466
Score = 24.9 bits (48), Expect(2) = 1e-022
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +3 / -3
Query: 546 EHRQVHRVGPLL 581
+ R VHRVGPLL
Sbjct: 258 QDRHVHRVGPLL 223
>gb|BM101447.2|BM101447 EBpi01_SQ003_O01_R pistil, 1 DPA, no treatment, cv Optic, EBpi01
Hordeum vulgare subsp. vulgare cDNA clone
EBpi01_SQ003_O01 5', mRNA sequence
Length = 452
Score = 125 bits (268), Expect = 2e-028
Identities = 49/72 (68%), Positives = 55/72 (76%)
Frame = -2 / +1
Query: 521 DVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANW 342
D+++ G WT MRESWGSIWRLDS HRL APFS+RI NESGKTLVA VIP NW
Sbjct: 118 DLMESGRGRRGGGRWTRMRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIPKNW 297
Query: 341 VPNTYYRSIIQY 306
PNT+YRSI+QY
Sbjct: 298 RPNTFYRSIVQY 333
Score = 100 bits (214), Expect(2) = 1e-022
Identities = 45/60 (75%), Positives = 45/60 (75%)
Frame = +1 / -1
Query: 307 YWMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDP 486
YW MER* VLG Q GMT ATSVLPDSL MR E GA RRW ES RQMDPHDSRI VH P
Sbjct: 332 YWTMER*KVLGLQFLGMTLRATSVLPDSLRMRMENGAWRRWLESRRQMDPHDSRIRVHRP 153
Score = 64.3 bits (134), Expect(2) = 3e-022
Identities = 25/33 (75%), Positives = 30/33 (90%)
Frame = -2 / +1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSG 483
EEGSN VY+A+LVE+E+GDGDVVQVDLME+ G
Sbjct: 43 EEGSNAVYMAILVEYENGDGDVVQVDLMESGRG 141
Score = 60.2 bits (125), Expect(2) = 3e-022
Identities = 28/44 (63%), Positives = 28/44 (63%)
Frame = -3 / +2
Query: 484 GRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
G GR CASRG PSG TP S R SP AS TSPARR P SS
Sbjct: 155 GGGRGCASRGDPSGASTPTIVSRRHSPSASATSPARRSSPATSS 286
Score = 24.9 bits (48), Expect(2) = 1e-022
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +3 / -3
Query: 546 EHRQVHRVGPLL 581
+ R VHRVGPLL
Sbjct: 78 QDRHVHRVGPLL 43
>gb|CA017263.1|CA017263 HV14A15u HV Hordeum vulgare subsp. vulgare cDNA clone HV14A15
3-PRIME, mRNA sequence
Length = 569
Score = 125 bits (268), Expect = 2e-028
Identities = 49/72 (68%), Positives = 55/72 (76%)
Frame = -2 / -2
Query: 521 DVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANW 342
D+++ G WT MRESWGSIWRLDS HRL APFS+RI NESGKTLVA VIP NW
Sbjct: 424 DLMESGRGRRGGGRWTRMRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIPKNW 245
Query: 341 VPNTYYRSIIQY 306
PNT+YRSI+QY
Sbjct: 244 RPNTFYRSIVQY 209
Score = 100 bits (214), Expect(2) = 1e-022
Identities = 45/60 (75%), Positives = 45/60 (75%)
Frame = +1 / +3
Query: 307 YWMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDP 486
YW MER* VLG Q GMT ATSVLPDSL MR E GA RRW ES RQMDPHDSRI VH P
Sbjct: 210 YWTMER*KVLGLQFLGMTLRATSVLPDSLRMRMENGAWRRWLESRRQMDPHDSRIRVHRP 389
Score = 64.3 bits (134), Expect(2) = 3e-022
Identities = 25/33 (75%), Positives = 30/33 (90%)
Frame = -2 / -2
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSG 483
EEGSN VY+A+LVE+E+GDGDVVQVDLME+ G
Sbjct: 499 EEGSNAVYMAILVEYENGDGDVVQVDLMESGRG 401
Score = 60.2 bits (125), Expect(2) = 3e-022
Identities = 28/44 (63%), Positives = 28/44 (63%)
Frame = -3 / -3
Query: 484 GRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
G GR CASRG PSG TP S R SP AS TSPARR P SS
Sbjct: 387 GGGRGCASRGDPSGASTPTIVSRRHSPSASATSPARRSSPATSS 256
Score = 24.9 bits (48), Expect(2) = 1e-022
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +3 / +2
Query: 546 EHRQVHRVGPLL 581
+ R VHRVGPLL
Sbjct: 464 QDRHVHRVGPLL 499
>gb|AJ461923.1|AJ461923 AJ461923 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200052C12F1, mRNA sequence
Length = 300
Score = 125 bits (267), Expect = 2e-028
Identities = 48/60 (80%), Positives = 51/60 (85%)
Frame = -2 / +3
Query: 485 GSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
G WT MRESWGSIWRLDS HRL APFS+RI NESGKTLVA VIP NW PNT+YRSI+QY
Sbjct: 6 GRWTRMRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIPKNWRPNTFYRSIVQY 185
Score = 100 bits (214), Expect = 5e-021
Identities = 45/60 (75%), Positives = 45/60 (75%)
Frame = +1 / -3
Query: 307 YWMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDP 486
YW MER* VLG Q GMT ATSVLPDSL MR E GA RRW ES RQMDPHDSRI VH P
Sbjct: 184 YWTMER*KVLGLQFLGMTLRATSVLPDSLRMRMENGAWRRWLESRRQMDPHDSRIRVHRP 5
>gb|CA001233.1|CA001233 HS18N14u HS Hordeum vulgare subsp. vulgare cDNA clone HS18N14
3-PRIME, mRNA sequence
Length = 539
Score = 119 bits (254), Expect(2) = 3e-028
Identities = 45/61 (73%), Positives = 51/61 (83%)
Frame = -2 / -2
Query: 488 SGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQ 309
SGSW MRESWGSIWR+DS HRL PFS+RIT++SGK LVAN VIPANW PNT YRS +Q
Sbjct: 538 SGSWMAMRESWGSIWRMDSNHRLQGPFSMRITSDSGKKLVANNVIPANWRPNTDYRSFVQ 359
Query: 308 Y 306
+
Sbjct: 358 F 356
Score = 106 bits (227), Expect(2) = 3e-025
Identities = 46/60 (76%), Positives = 48/60 (80%)
Frame = +1 / +3
Query: 310 WMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSRIGVHDPE 489
W ER* VLG QLAGMT LATS LP+SLVMR EKG RRW ES RQMDPHDSRI +HDPE
Sbjct: 360 WTKER*SVLGLQLAGMTLLATSFLPESLVMRMEKGPWRRWLESIRQMDPHDSRIAIHDPE 539
Score = 28.6 bits (56), Expect(2) = 3e-025
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +1 / +3
Query: 40 IALVHYMHGQWAGKHLQINL 99
+ +VH QWAG+H+Q L
Sbjct: 57 LCMVHRTQSQWAGEHMQTML 116
Score = 26.7 bits (52), Expect(2) = 3e-028
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -2 / -2
Query: 95 LICRCFPAHCPCM*CT 48
++C C PAH C+ CT
Sbjct: 112 MVCMCSPAH*LCVRCT 65
>gb|BE454367.2|BE454367 HVSMEh0093N18f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0093N18f, mRNA sequence
Length = 870
Score = 79.9 bits (168), Expect(2) = 7e-028
Identities = 28/59 (47%), Positives = 39/59 (66%)
Frame = -2 / +1
Query: 485 GSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQ 309
G WT MRESWGS+WRL+ HRL APF +++ ++ G+TL + +P W P T R + Q
Sbjct: 289 GKWTRMRESWGSVWRLNCNHRLQAPFCIQVRDKCGETLGGSMGVPGRWRPGTLCRCVEQ 465
Score = 63.4 bits (132), Expect(2) = 7e-028
Identities = 26/30 (86%), Positives = 28/30 (93%)
Frame = -2 / +1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEA 492
EE SNPVY AVLVE+EDGDGDVVQVDLME+
Sbjct: 184 EEYSNPVYFAVLVEYEDGDGDVVQVDLMES 273
>gb|BQ661556.1|BQ661556 HM03P13u HM Hordeum vulgare subsp. vulgare cDNA clone HM03P13
3-PRIME, mRNA sequence
Length = 442
Score = 122 bits (260), Expect = 2e-027
Identities = 46/54 (85%), Positives = 52/54 (96%)
Frame = -2 / -2
Query: 467 RESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
RESWGSIWRLDSGHRL APFS+RITNESGKTLVA++VIPANW P+T+YRSI+QY
Sbjct: 441 RESWGSIWRLDSGHRLQAPFSMRITNESGKTLVADKVIPANWAPSTFYRSIVQY 280
Score = 107 bits (228), Expect = 6e-023
Identities = 46/54 (85%), Positives = 47/54 (87%)
Frame = +1 / +2
Query: 307 YWMMER**VLGTQLAGMTWLATSVLPDSLVMRREKGAARRWPESSRQMDPHDSR 468
YW MER* VLG QLAGMT ATSVLPDSLVMR EKGA RRWPESSRQ+DPHDSR
Sbjct: 281 YWTMER*KVLGAQLAGMTLSATSVLPDSLVMRMEKGAWRRWPESSRQIDPHDSR 442
>gb|CB881625.1|CB881625 HM10G06w HM Hordeum vulgare subsp. vulgare cDNA clone HM10G06
3-PRIME, mRNA sequence
Length = 588
Score = 94.1 bits (199), Expect(2) = 9e-027
Identities = 35/59 (59%), Positives = 45/59 (76%)
Frame = -2 / -1
Query: 479 WTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY* 303
W PM SWG+IWR D + + APFS+R+T+ESGK L+A VIPANW P+T Y+S IQ+*
Sbjct: 378 WIPMTLSWGAIWRWDGANAIKAPFSIRLTSESGKKLIAQDVIPANWKPDTVYQSNIQF* 202
Score = 45.5 bits (93), Expect(2) = 9e-027
Identities = 17/32 (53%), Positives = 25/32 (78%)
Frame = -2 / -1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANS 486
E GSNP YLA+LV+F DGD+VQ+++ E ++
Sbjct: 483 ETGSNPNYLAILVKFVADDGDIVQMEIQEKSA 388
>gb|CB880524.1|CB880524 HM06N05w HM Hordeum vulgare subsp. vulgare cDNA clone HM06N05
3-PRIME, mRNA sequence
Length = 545
Score = 94.1 bits (199), Expect(2) = 9e-027
Identities = 35/59 (59%), Positives = 45/59 (76%)
Frame = -2 / -2
Query: 479 WTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY* 303
W PM SWG+IWR D + + APFS+R+T+ESGK L+A VIPANW P+T Y+S IQ+*
Sbjct: 388 WIPMTLSWGAIWRWDGANAIKAPFSIRLTSESGKKLIAQDVIPANWKPDTVYQSNIQF* 212
Score = 45.5 bits (93), Expect(2) = 9e-027
Identities = 17/32 (53%), Positives = 25/32 (78%)
Frame = -2 / -2
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANS 486
E GSNP YLA+LV+F DGD+VQ+++ E ++
Sbjct: 493 ETGSNPNYLAILVKFVADDGDIVQMEIQEKSA 398
>gb|BQ661443.1|BQ661443 HM03I08u HM Hordeum vulgare subsp. vulgare cDNA clone HM03I08
3-PRIME, mRNA sequence
Length = 520
Score = 94.1 bits (199), Expect(2) = 9e-027
Identities = 35/59 (59%), Positives = 45/59 (76%)
Frame = -2 / -3
Query: 479 WTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY* 303
W PM SWG+IWR D + + APFS+R+T+ESGK L+A VIPANW P+T Y+S IQ+*
Sbjct: 377 WIPMTLSWGAIWRWDGANAIKAPFSIRLTSESGKKLIAQDVIPANWKPDTVYQSNIQF* 201
Score = 45.5 bits (93), Expect(2) = 9e-027
Identities = 17/32 (53%), Positives = 25/32 (78%)
Frame = -2 / -3
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANS 486
E GSNP YLA+LV+F DGD+VQ+++ E ++
Sbjct: 482 ETGSNPNYLAILVKFVADDGDIVQMEIQEKSA 387
>gb|BI959138.1|BI959138 HVSMEn0018F10f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0018F10f, mRNA sequence
Length = 669
Score = 79.0 bits (166), Expect(2) = 2e-026
Identities = 30/35 (85%), Positives = 34/35 (97%)
Frame = -2 / +3
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGSW 477
E+GSNPVY AVLVE+EDGDGD+VQVDLMEANSG+W
Sbjct: 483 EQGSNPVYFAVLVEYEDGDGDLVQVDLMEANSGTW 587
Score = 59.7 bits (124), Expect(2) = 2e-026
Identities = 21/25 (84%), Positives = 24/25 (96%)
Frame = -2 / +1
Query: 476 TPMRESWGSIWRLDSGHRLAAPFSL 402
TPMRESWGSIWR++SGHRL APFS+
Sbjct: 589 TPMRESWGSIWRVESGHRLQAPFSM 663
>gb|CK123980.1|CK123980 BES1824106f16 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010F166 5-PRIME, mRNA sequence
Length = 750
Score = 94.1 bits (199), Expect(2) = 1e-021
Identities = 38/57 (66%), Positives = 42/57 (73%)
Frame = -2 / +1
Query: 521 DVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIP 351
D+++ G WT MRESWGSIWRLDS HRL APFS+RI NESGKTLVA VIP
Sbjct: 493 DLMESGRGRRGGGRWTRMRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIP 663
Score = 64.3 bits (134), Expect(3) = 2e-026
Identities = 25/33 (75%), Positives = 30/33 (90%)
Frame = -2 / +1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSG 483
EEGSN VY+A+LVE+E+GDGDVVQVDLME+ G
Sbjct: 418 EEGSNAVYMAILVEYENGDGDVVQVDLMESGRG 516
Score = 58.3 bits (121), Expect(3) = 2e-026
Identities = 27/44 (61%), Positives = 27/44 (61%)
Frame = -3 / +2
Query: 484 GRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARRWWPTRSS 353
G GR CASRG PSG TP S R SP AS TSP RR P SS
Sbjct: 530 GGGRGCASRGDPSGASTPTIVSRRHSPSASATSPXRRSSPATSS 661
Score = 33.6 bits (67), Expect(3) = 2e-026
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = -2 / +2
Query: 353 PANWVPNTYYRSIIQY 306
P NW NT+YRSI+QY
Sbjct: 662 PKNWRXNTFYRSIVQY 709
Score = 28.6 bits (56), Expect(2) = 1e-021
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -1 / +1
Query: 354 PGQLGSQHLLPFHHPV 307
P +L QHLLP H PV
Sbjct: 661 PXKLEXQHLLPLHRPV 708
>gb|AV918397.1|AV918397 AV918397 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20o17 3', mRNA sequence
Length = 498
Score = 116 bits (248), Expect = 1e-025
Identities = 45/55 (81%), Positives = 48/55 (87%)
Frame = -2 / -1
Query: 470 MRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
MRESWGSIWRLDS HRL APFS+RI NESGKTLVA VIP NW PNT+YRSI+QY
Sbjct: 498 MRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIPKNWRPNTFYRSIVQY 334
>gb|CB876346.1|CB876346 HX11A03w HX Hordeum vulgare subsp. vulgare cDNA clone HX11A03
3-PRIME, mRNA sequence
Length = 543
Score = 116 bits (248), Expect = 1e-025
Identities = 45/55 (81%), Positives = 48/55 (87%)
Frame = -2 / -1
Query: 470 MRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
MRESWGSIWRLDS HRL APFS+RI NESGKTLVA VIP NW PNT+YRSI+QY
Sbjct: 543 MRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIPKNWRPNTFYRSIVQY 379
>gb|BJ463021.1|BJ463021 BJ463021 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags25g15 5', mRNA sequence
Length = 532
Score = 115 bits (246), Expect = 2e-025
Identities = 43/75 (57%), Positives = 57/75 (76%)
Frame = -2 / +3
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSL 402
++GSNP Y L+EFED DGD+ V L EA SG+WT M + WG++WRL++G+RL APFSL
Sbjct: 303 DQGSNPFYFKTLIEFEDDDGDLKAVALKEAGSGAWTQMTQDWGALWRLNNGNRLRAPFSL 482
Query: 401 RITNESGKTLVANQV 357
R+T +SG+ LV N V
Sbjct: 483 RLTXDSGRKLVXNNV 527
>gb|BU990646.1|BU990646 HF25K12r HF Hordeum vulgare subsp. vulgare cDNA clone HF25K12
5-PRIME, mRNA sequence
Length = 281
Score = 115 bits (245), Expect = 3e-025
Identities = 45/68 (66%), Positives = 50/68 (73%)
Frame = -2 / +3
Query: 521 DVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANW 342
D+++ G WT MRESWGSIWRLDS HRL APFS+RI NESGKTLV VIP NW
Sbjct: 78 DLMESGRGRRGGGRWTRMRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVXRNVIPKNW 257
Query: 341 VPNTYYRS 318
PNT+YRS
Sbjct: 258 RPNTFYRS 281
Score = 59.7 bits (124), Expect(3) = 9e-025
Identities = 23/31 (74%), Positives = 28/31 (90%)
Frame = -2 / +3
Query: 575 GSNPVYLAVLVEFEDGDGDVVQVDLMEANSG 483
GSN VY+A+LVE+E+GDGDVVQVDLME+ G
Sbjct: 9 GSNAVYMAILVEYENGDGDVVQVDLMESGRG 101
Score = 56.5 bits (117), Expect(3) = 9e-025
Identities = 25/37 (67%), Positives = 25/37 (67%)
Frame = -3 / +1
Query: 484 GRGRRCASRGGPSGGWTPATASPRPSPCASPTSPARR 374
G GR CASRG PSG TP S R SP AS TSPARR
Sbjct: 115 GGGRGCASRGDPSGASTPTIVSRRHSPSASATSPARR 225
Score = 32.2 bits (64), Expect(3) = 9e-025
Identities = 14/24 (58%), Positives = 16/24 (66%)
Frame = -1 / +2
Query: 393 QRVRQDAGGQPGHPGQLGSQHLLP 322
QRVRQDA H +L +QHLLP
Sbjct: 206 QRVRQDARRPQRHSQELEAQHLLP 277
>gb|BM442090.2|BM442090 EBan01_SQ002_C16_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_C16 5', mRNA sequence
Length = 389
Score = 114 bits (244), Expect = 4e-025
Identities = 41/88 (46%), Positives = 59/88 (67%)
Frame = -2 / +2
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSL 402
E+GS+P YLA++++F GDGDVV VD+ W ++ESWG++WR+D+ +L PFS+
Sbjct: 56 EKGSSPNYLALVIKFLQGDGDVVAVDIKPKGKDKWIELKESWGAVWRIDTPDKLIGPFSV 235
Query: 401 RITNESGKTLVANQVIPANWVPNTYYRS 318
R T E G VA VIP W P+T+Y +
Sbjct: 236 RYTTEGGTKTVAEDVIPEGWKPDTFYET 319
>gb|AJ463236.1|AJ463236 AJ463236 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200037A04F1, mRNA sequence
Length = 420
Score = 76.7 bits (161), Expect(2) = 4e-025
Identities = 28/43 (65%), Positives = 37/43 (86%)
Frame = -2 / +1
Query: 560 YLAVLVEFEDGDGDVVQVDLMEANSGSWTPMRESWGSIWRLDS 432
YL VL+EFED DGD+ VDLMEAN G+WTPM ++WG+++RL+S
Sbjct: 1 YLEVLIEFEDDDGDLNTVDLMEANCGTWTPMVQNWGALYRLNS 129
Score = 57.4 bits (119), Expect(2) = 4e-025
Identities = 21/44 (47%), Positives = 30/44 (68%)
Frame = -2 / +1
Query: 437 DSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
++G L PFSLR+T++SG+ LV N VIP +W YRS++ Y
Sbjct: 130 NTGKPLRGPFSLRLTSDSGRKLVVNNVIPVSWKAGATYRSLVNY 261
>gb|AV918336.1|AV918336 AV918336 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20l09 3', mRNA sequence
Length = 601
Score = 114 bits (243), Expect = 5e-025
Identities = 44/54 (81%), Positives = 47/54 (87%)
Frame = -2 / -2
Query: 467 RESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
RESWGSIWRLDS HRL APFS+RI NESGKTLVA VIP NW PNT+YRSI+QY
Sbjct: 600 RESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIPKNWRPNTFYRSIVQY 439
>gb|CB881182.1|CB881182 HM09A07w HM Hordeum vulgare subsp. vulgare cDNA clone HM09A07
3-PRIME, mRNA sequence
Length = 680
Score = 112 bits (240), Expect = 1e-024
Identities = 42/88 (47%), Positives = 58/88 (65%)
Frame = -2 / -3
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLMEANSGSWTPMRESWGSIWRLDSGHRLAAPFSL 402
E+GS+P YLA++V+F GDGDVV VD+ W ++ESWG++WR+D+ +L PFS+
Sbjct: 522 EKGSSPNYLALVVKFLQGDGDVVAVDIKPKGKDKWIELKESWGAVWRIDTPDKLIGPFSV 343
Query: 401 RITNESGKTLVANQVIPANWVPNTYYRS 318
R T E G VA VIP W P+T Y +
Sbjct: 342 RYTTEGGTKTVAEDVIPEGWKPDTSYET 259
>gb|CA001449.1|CA001449 HS17P08u HS Hordeum vulgare subsp. vulgare cDNA clone HS17P08
3-PRIME, mRNA sequence
Length = 518
Score = 106 bits (226), Expect(2) = 2e-024
Identities = 40/54 (74%), Positives = 46/54 (85%)
Frame = -2 / -2
Query: 467 RESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
RESWGSIWR+DS HRL PFS+RIT++SGK LVAN VIPANW PNT YRS +Q+
Sbjct: 517 RESWGSIWRMDSNHRLQGPFSMRITSDSGKKLVANNVIPANWRPNTDYRSFVQF 356
Score = 26.7 bits (52), Expect(2) = 2e-024
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -2 / -2
Query: 95 LICRCFPAHCPCM*CT 48
++C C PAH C+ CT
Sbjct: 112 MVCMCSPAH*LCVRCT 65
>gb|CK122737.1|CK122737 BES1824105j22 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010J225 5-PRIME, mRNA sequence
Length = 776
Score = 69.8 bits (146), Expect(2) = 3e-024
Identities = 29/46 (63%), Positives = 32/46 (69%)
Frame = -2 / +3
Query: 494 ANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQV 357
A +G WT M ESWGSIW LDS +RL P S+RI NESGK L A V
Sbjct: 639 AAAGRWTRMPESWGSIWGLDSXNRLQXPXSIRIRNESGKKLXARNV 776
Score = 61.6 bits (128), Expect(2) = 3e-024
Identities = 24/29 (82%), Positives = 28/29 (96%)
Frame = -2 / +1
Query: 581 EEGSNPVYLAVLVEFEDGDGDVVQVDLME 495
EEGSN VY+A+LVE+E+GDGDVVQVDLME
Sbjct: 535 EEGSNAVYMAILVEYENGDGDVVQVDLME 621
>gb|BQ665573.1|BQ665573 HX04D14u HX Hordeum vulgare subsp. vulgare cDNA clone HX04D14
3-PRIME, mRNA sequence
Length = 472
Score = 111 bits (237), Expect = 3e-024
Identities = 43/63 (68%), Positives = 50/63 (79%)
Frame = -2 / -3
Query: 494 ANSGSWTPMRESWGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSI 315
A +G W PM ESWGSIWR+D+ + PFSLRITNESGKTLVA+QVIPA+W PN Y SI
Sbjct: 434 APTGEWVPMTESWGSIWRMDTRRPMQGPFSLRITNESGKTLVADQVIPADWEPNEIYSSI 255
Query: 314 IQY 306
IQ+
Sbjct: 254 IQF 246
>gb|BG299356.2|BG299356 HVSMEa0019J09f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0019J09f, mRNA sequence
Length = 564
Score = 111 bits (236), Expect = 5e-024
Identities = 41/51 (80%), Positives = 46/51 (90%)
Frame = -2 / +1
Query: 458 WGSIWRLDSGHRLAAPFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
WGS+WRLDS HRL APFS+RI NESGKTLVAN+VIPANW PNT+YRS +QY
Sbjct: 1 WGSVWRLDSNHRLQAPFSIRIRNESGKTLVANKVIPANWRPNTFYRSFVQY 153
>gb|BQ658207.1|BQ658207 HA05E07u HA Hordeum vulgare subsp. vulgare cDNA clone HA05E07
3-PRIME, mRNA sequence
Length = 469
Score = 65.7 bits (137), Expect(3) = 9e-024
Identities = 24/36 (66%), Positives = 31/36 (86%)
Frame = -2 / -2
Query: 413 PFSLRITNESGKTLVANQVIPANWVPNTYYRSIIQY 306
PFSLR+TNESGKTLVA++VIP +W PN Y S++Q+
Sbjct: 303 PFSLRVTNESGKTLVADKVIPGDWKPNKTYSSLVQF 196
Score = 44.6 bits (91), Expect(3) = 9e-024
Identities = 13/19 (68%), Positives = 17/19 (89%)
Frame = -2 / -1
Query: 488 SGSWTPMRESWGSIWRLDS 432
+G W PM+ESWGSIWR+D+
Sbjct: 379 TGEWVPMKESWGSIWRMDA 323
Score = 36.8 bits (74), Expect(3) = 9e-024
Identities = 13/21 (61%), Positives = 18/21 (85%)
Frame = -2 / -1
Query: 545 VEFEDGDGDVVQVDLMEANSG 483
VE+E+GDGDV Q+D+ME+ G
Sbjct: 469 VEYENGDGDVKQLDIMESRPG 407
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 288,804,326
Number of Sequences: 312970
Number of extensions: 6010300
Number of successful extensions: 238704
Number of sequences better than 1.0e-020: 76
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6023172678
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)