TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419355.2.1
(1174 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CK123665.1|CK123665 BES1824105a07 BES1824 Hordeum vulgar... 402 e-111
gb|BE060099.2|BE060099 HVSMEg0011H06f Hordeum vulgare pre-a... 372 e-102
gb|BF260284.3|BF260284 HVSMEf0021J12f Hordeum vulgare seedl... 285 2e-086
gb|BF254000.2|BF254000 HVSMEf0002L12f Hordeum vulgare seedl... 274 8e-073
gb|AV834569.1|AV834569 AV834569 K. Sato unpublished cDNA li... 241 7e-063
gb|CV054720.1|CV054720 BNEL112e7 Barley EST endosperm libra... 142 5e-059
gb|CV063832.1|CV063832 BNEL93f7 Barley EST endosperm librar... 142 3e-058
gb|AV916868.1|AV916868 AV916868 K. Sato unpublished cDNA li... 142 2e-055
gb|CK124077.1|CK124077 BES1824106o13 BES1824 Hordeum vulgar... 114 2e-054
gb|CA011697.1|CA011697 HT03E21r HT Hordeum vulgare subsp. v... 142 7e-054
gb|BU978492.1|BU978492 HA13G08r HA Hordeum vulgare subsp. v... 192 3e-048
gb|BG416606.2|BG416606 HVSMEk0013I06f Hordeum vulgare testa... 115 9e-048
gb|CA012675.1|CA012675 HT06B18r HT Hordeum vulgare subsp. v... 118 2e-045
gb|AV916050.1|AV916050 AV916050 K. Sato unpublished cDNA li... 95 7e-044
gb|CA006036.1|CA006036 HU05K13u HU Hordeum vulgare subsp. v... 171 7e-042
gb|CA031881.1|CA031881 HX11G09r HX Hordeum vulgare subsp. v... 163 1e-039
gb|AV915850.1|AV915850 AV915850 K. Sato unpublished cDNA li... 87 3e-039
gb|BE060343.3|BE060343 HVSMEg0012A18f Hordeum vulgare pre-a... 116 5e-037
gb|BJ447407.1|BJ447407 BJ447407 K. Sato unpublished cDNA li... 118 7e-037
gb|BJ463449.1|BJ463449 BJ463449 K. Sato unpublished cDNA li... 122 4e-035
gb|AV924480.1|AV924480 AV924480 K. Sato unpublished cDNA li... 92 7e-035
gb|AV924479.1|AV924479 AV924479 K. Sato unpublished cDNA li... 92 7e-035
gb|BE437488.1|BE437488 SFR004.C06F990621 ITEC SFR Barley Le... 138 1e-034
gb|BE195361.2|BE195361 HVSMEh0089A10f Hordeum vulgare 5-45 ... 95 1e-033
gb|BF616546.2|BF616546 HVSMEc0010G23f Hordeum vulgare seedl... 116 1e-033
gb|CA026989.1|CA026989 HZ57I24r HZ Hordeum vulgare subsp. v... 140 1e-032
gb|BU989522.1|BU989522 HF21P14r HF Hordeum vulgare subsp. v... 80 9e-032
gb|AV913885.1|AV913885 AV913885 K. Sato unpublished cDNA li... 87 4e-031
gb|BG369694.1|BG369694 HVSMEi0025I17f Hordeum vulgare 20 DA... 122 9e-031
gb|BG344809.1|BG344809 HVSMEg0016C19f Hordeum vulgare pre-a... 91 1e-030
gb|BQ472612.1|BQ472612 HB01P21r BC Hordeum vulgare subsp. v... 91 1e-030
gb|BQ472874.1|BQ472874 HB01D18r BC Hordeum vulgare subsp. v... 91 1e-030
gb|CK124523.1|CK124523 BES1824109h02 BES1824 Hordeum vulgar... 105 2e-030
gb|BM098487.2|BM098487 EBem08_SQ003_D06_R embryo, 40 DPA, n... 122 2e-030
gb|BI950087.1|BI950087 HVSMEl0018H22f Hordeum vulgare spike... 105 3e-030
gb|BJ450504.1|BJ450504 BJ450504 K. Sato unpublished cDNA li... 59 7e-030
gb|BF260432.3|BF260432 HVSMEf0021P16f Hordeum vulgare seedl... 109 1e-029
gb|BQ459038.1|BQ459038 HA02H18r HA Hordeum vulgare subsp. v... 100 5e-029
gb|BQ469784.1|BQ469784 HZ01N19r HZ Hordeum vulgare subsp. v... 127 1e-028
gb|BE421366.1|BE421366 HWM008.E11 ITEC HWM Barley Leaf Libr... 78 2e-028
gb|AV916179.1|AV916179 AV916179 K. Sato unpublished cDNA li... 105 5e-028
gb|BU979682.1|BU979682 HA16O21r HA Hordeum vulgare subsp. v... 122 5e-027
gb|CA011901.1|CA011901 HT03N20r HT Hordeum vulgare subsp. v... 120 1e-026
gb|BE456133.3|BE456133 HVSMEg0019P07f Hordeum vulgare pre-a... 119 3e-026
gb|BQ768557.1|BQ768557 EBro08_SQ012_E03_R root, 3 week, dro... 117 9e-026
gb|CA022858.1|CA022858 HZ44I22r HZ Hordeum vulgare subsp. v... 117 9e-026
gb|BU988151.1|BU988151 HF16M22r HF Hordeum vulgare subsp. v... 91 6e-025
gb|BF622331.2|BF622331 HVSMEa0002M16f Hordeum vulgare seedl... 86 1e-024
gb|BQ767779.1|BQ767779 EBro08_SQ009_G20_R root, 3 week, dro... 80 2e-024
gb|AV926794.1|AV926794 AV926794 K. Sato unpublished cDNA li... 105 3e-024
gb|CV055826.1|CV055826 BNEL123h3 Barley EST endosperm libra... 111 6e-024
gb|CK125211.1|CK125211 BES1824107g08 BES1824 Hordeum vulgar... 108 6e-024
gb|CK123916.1|CK123916 BES1824106c06 BES1824 Hordeum vulgar... 105 6e-024
gb|CV053850.1|CV053850 BNEL103h12 Barley EST endosperm libr... 108 6e-024
gb|CV054070.1|CV054070 BNEL106d4 Barley EST endosperm libra... 108 6e-024
gb|AL505423.1|AL505423 AL505423 Hordeum vulgare Barke roots... 105 6e-024
gb|CV060466.1|CV060466 BNEL58d7 Barley EST endosperm librar... 108 6e-024
gb|BU984301.1|BU984301 HF03I21r HF Hordeum vulgare subsp. v... 108 6e-024
gb|BF624269.1|BF624269 HVSMEa0013E15f Hordeum vulgare seedl... 108 6e-024
gb|BJ464155.1|BJ464155 BJ464155 K. Sato unpublished cDNA li... 105 6e-024
gb|AV925147.1|AV925147 AV925147 K. Sato unpublished cDNA li... 84 6e-024
gb|BJ462966.1|BJ462966 BJ462966 K. Sato unpublished cDNA li... 78 6e-024
gb|BE195511.3|BE195511 HVSMEh0089E06f Hordeum vulgare 5-45 ... 106 1e-023
gb|BJ447550.1|BJ447550 BJ447550 K. Sato unpublished cDNA li... 106 1e-023
gb|CA013311.1|CA013311 HT08A09r HT Hordeum vulgare subsp. v... 82 2e-023
gb|BI948209.1|BI948209 HVSMEl0008J18f Hordeum vulgare spike... 84 4e-023
gb|AV915769.1|AV915769 AV915769 K. Sato unpublished cDNA li... 93 4e-023
gb|BU977459.1|BU977459 HA11H12r HA Hordeum vulgare subsp. v... 108 7e-023
gb|CV057382.1|CV057382 BNEL27b8 Barley EST endosperm librar... 108 7e-023
gb|BQ458815.1|BQ458815 HA04C06r HA Hordeum vulgare subsp. v... 87 1e-022
gb|BF255228.2|BF255228 HVSMEf0006E20f Hordeum vulgare seedl... 99 2e-022
gb|CA031709.1|CA031709 HX10O12r HX Hordeum vulgare subsp. v... 104 2e-022
gb|AW982654.1|AW982654 HVSMEg0003N16f Hordeum vulgare pre-a... 106 2e-022
gb|BJ465012.1|BJ465012 BJ465012 K. Sato unpublished cDNA li... 106 2e-022
gb|AV933996.1|AV933996 AV933996 K. Sato unpublished cDNA li... 105 3e-022
gb|BQ458577.1|BQ458577 HA04N11r HA Hordeum vulgare subsp. v... 100 5e-022
gb|BG300235.1|BG300235 HVSMEb0011L04f Hordeum vulgare seedl... 100 5e-022
gb|BU970617.1|BU970617 HB15C22r BC Hordeum vulgare subsp. v... 100 5e-022
gb|AV914074.1|AV914074 AV914074 K. Sato unpublished cDNA li... 100 5e-022
gb|BU980835.1|BU980835 HA21N10r HA Hordeum vulgare subsp. v... 100 5e-022
gb|BQ468899.1|BQ468899 HM02L10r HM Hordeum vulgare subsp. v... 105 5e-022
gb|BQ472290.1|BQ472290 HV04P12r HV Hordeum vulgare subsp. v... 105 5e-022
gb|BJ465339.1|BJ465339 BJ465339 K. Sato unpublished cDNA li... 100 6e-022
gb|CA014679.1|CA014679 HT12A17r HT Hordeum vulgare subsp. v... 100 6e-022
gb|BQ464579.1|BQ464579 HF02K23r HF Hordeum vulgare subsp. v... 100 6e-022
gb|BU968719.1|BU968719 HB08G03r BC Hordeum vulgare subsp. v... 100 6e-022
gb|BJ454472.1|BJ454472 BJ454472 K. Sato unpublished cDNA li... 100 6e-022
gb|BU979675.1|BU979675 HA16O11r HA Hordeum vulgare subsp. v... 89 6e-022
gb|BF257803.3|BF257803 HVSMEf0013P05f Hordeum vulgare seedl... 105 7e-022
gb|BI948208.1|BI948208 HVSMEl0008J16f Hordeum vulgare spike... 80 8e-022
gb|BG343957.1|BG343957 HVSMEg0007D17f Hordeum vulgare pre-a... 95 2e-021
gb|BI952155.1|BI952155 HVSMEm0004N04f Hordeum vulgare green... 100 2e-021
gb|BM444302.1|BM444302 EBem09_SQ004_M04_R embryo, 1 Day ger... 100 3e-021
gb|CB878656.1|CB878656 HP09F15T HP Hordeum vulgare subsp. v... 100 3e-021
gb|BJ463068.1|BJ463068 BJ463068 K. Sato unpublished cDNA li... 97 4e-021
gb|AV913842.1|AV913842 AV913842 K. Sato unpublished cDNA li... 97 6e-021
gb|AV915313.1|AV915313 AV915313 K. Sato unpublished cDNA li... 97 6e-021
gb|BF254653.2|BF254653 HVSMEf0004K07f Hordeum vulgare seedl... 101 6e-021
gb|BJ464645.1|BJ464645 BJ464645 K. Sato unpublished cDNA li... 101 8e-021
gb|BU978903.1|BU978903 HA14J14r HA Hordeum vulgare subsp. v... 101 8e-021
gb|BU979336.1|BU979336 HA15N06r HA Hordeum vulgare subsp. v... 101 8e-021
gb|BU985834.1|BU985834 HF08M10r HF Hordeum vulgare subsp. v... 101 8e-021
gb|BU986432.1|BU986432 HF11N06r HF Hordeum vulgare subsp. v... 101 8e-021
>gb|CK123665.1|CK123665 BES1824105a07 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010A075 5-PRIME, mRNA sequence
Length = 670
Score = 402 bits (873), Expect = e-111
Identities = 173/197 (87%), Positives = 179/197 (90%)
Frame = +3 / +1
Query: 183 VLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASRRLKTELLIQMDGLTKTNDLVFVL 362
VLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASRRLKTELLIQMDGLTKT DLVFVL
Sbjct: 16 VLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASRRLKTELLIQMDGLTKTKDLVFVL 195
Query: 363 AATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIPYDILVEKTEGY 542
AATNLPWELDAAMLRRLEKRILVPLPE EAR +MF+ELLPA TS LE+PYDILVEKTEGY
Sbjct: 196 AATNLPWELDAAMLRRLEKRILVPLPEAEARHSMFKELLPAMTSNLEVPYDILVEKTEGY 375
Query: 543 SGSDIRLVCKEAAMQPLRRLMSVLEASDXXXXXXXXXXXGPLKPDDIELALRNTRPSAHL 722
SGSDIRLVCKEAAMQPLRRLMSVLEASD GPLKPDD+ELAL NTRPSAHL
Sbjct: 376 SGSDIRLVCKEAAMQPLRRLMSVLEASDELVPEEELPEVGPLKPDDVELALSNTRPSAHL 555
Query: 723 HAHRYEKFNQDYGSHVV 773
AHRYEKFNQDYGSH++
Sbjct: 556 QAHRYEKFNQDYGSHIL 606
Score = 336 bits (728), Expect = 1e-091
Identities = 152/195 (77%), Positives = 159/195 (81%)
Frame = -1 / -3
Query: 766 WLP*SWLNFS*RCAWRCADGLVFLNASSIXXXXXXXXXXXXXXXXXXXXXSRTDMRRLSG 587
WLP*SWLNFS RCAWRCADGLV L ASS SRTDMRRL+G
Sbjct: 599 WLP*SWLNFSYRCAWRCADGLVLLKASSTSSGLRGPTSGNSSSGTSSSLASRTDMRRLNG 420
Query: 586 CMAASLHTRRISEPE*PSVFSTSMSYGISNFEVVAGRSSSNIACLASPSGSGTRIRFSSR 407
C+AASLHTRRISEPE PSVFST +SYG S FEV+AGRSS N+ CLAS SGSGTRIRFSSR
Sbjct: 419 CIAASLHTRRISEPEYPSVFSTKISYGTSRFEVIAGRSSLNMECLASASGSGTRIRFSSR 240
Query: 406 RSMAASNSQGRFVAASTNTKSFVFVKPSI*ISSSVFKRRLASCSLRASPRWLMIASISSR 227
RS+AASNS GRFVAASTNTKS VFVKPSI*ISSSVFKRRLASCSLRASPR L+IASISSR
Sbjct: 239 RSIAASNSHGRFVAASTNTKSLVFVKPSI*ISSSVFKRRLASCSLRASPR*LIIASISSR 60
Query: 226 NIVDGA*CLASSNRT 182
NIVDGA*CLASS RT
Sbjct: 59 NIVDGA*CLASSKRT 15
Score = 314 bits (681), Expect = 4e-085
Identities = 131/196 (66%), Positives = 152/196 (77%)
Frame = -3 / -2
Query: 770 DMATVVLVELLIAMRMEMC*RPSVP*CKFNIIRFQRANLRQFFLRNQLVTGFKN*HETSQ 591
+MA VVLV+ I M +EMC*RP V KF+++RF+RANLRQ FL +QL+TGF+N*HET Q
Sbjct: 603 NMAPVVLVKFFIPMCLEMC*RPCVAQSKFHVVRFERANLRQLFLWHQLITGFEN*HETPQ 424
Query: 590 WLHGSFFAHETNI*TRVTFGLFN*YVVRDF*L*GSCWQKFLKHRLPCFTFRKRYKNPLLK 411
WLH S FAH+TNI TRV F LFN* ++ D *+*G C QKFLKH +PCF FRK YKNPLLK
Sbjct: 423 WLHSSLFAHKTNIRTRVPFSLFN*NIIWDL*I*GHCGQKFLKHGMPCFGFRKWYKNPLLK 244
Query: 410 PAEHGCIQFPGKICSCKYKYQVVCLCQTIHLNK*LSLQATTSLMLTASFTTLADDSIYFI 231
P EH CIQFP IC CK+KY V CQ IHLNK* SLQA+TS ML SFTTL ++SI I
Sbjct: 243 PTEHSCIQFPW*ICCCKHKY*VFGFCQAIHLNK*FSLQASTSFMLATSFTTLTNNSINLI 64
Query: 230 KEYCGWCMMPSKLKQN 183
K+YCGWCMM KLK+N
Sbjct: 63 KKYCGWCMMSCKLKKN 16
Score = 264 bits (571), Expect = 7e-070
Identities = 132/206 (64%), Positives = 142/206 (68%)
Frame = -2 / -1
Query: 801 DNKIMLALNKRHGYRSPG*TSHSDAHGDVLTA*CSLMQVQYHQVSEGQPQAVLPPEPTRH 622
D +++ L K +G RSPG* H+D GDVLTA C QV QV EGQPQA LP P H
Sbjct: 634 DRALLILLRKEYGSRSPG*IFHTDVLGDVLTALCCSKQVPRRQV*EGQPQATLPLAPAHH 455
Query: 621 WLQELT*DVSVVAWQLLCTRDEYLNQSNLRSFQLVCRTGFLTLR*LLAEVPQTSLALLHL 442
WL+ELT*D S+ A Q LCT+DEY NQS L+SFQL G L LR L AEVP T ALL L
Sbjct: 454 WLRELT*DASMAA*QPLCTQDEYPNQSTLQSFQLKYHMGPLDLRSLRAEVP*TWNALLRL 275
Query: 441 QEAVQESASQAGGAWLHPIPREDL*LQVQIPSRLSLSNHPSE*VAQSSSDD*PHAHCELH 262
QE VQESASQA GA LHPIP DL LQ QI S LS+HPSE*V QSSS D* HA ELH
Sbjct: 274 QEVVQESASQADGA*LHPIPMVDLLLQAQILSLWFLSSHPSE*VVQSSSVD*LHARYELH 95
Query: 261 HAG***HLFHQGILWMVHDA*QAQTE 184
HA ***H HQ ILWMVHD QAQ E
Sbjct: 94 HAD***HQSHQEILWMVHDVLQAQKE 17
Score = 238 bits (514), Expect(2) = 8e-065
Identities = 109/164 (66%), Positives = 123/164 (75%)
Frame = +1 / +2
Query: 283 RLVVA*RLSYLFRWMV*QRQTTWYLYLQLQIFPGNWMQPCSAGLRSGFLYRFLKVKQGKR 462
+LV A*RL+YLFRWM *Q+ T YL LQ QI+ GNWMQ CS GLRSGFLY FLK KQG
Sbjct: 116 KLVDA*RLNYLFRWMA*QKPKT*YLCLQQQIYHGNWMQLCSVGLRSGFLYHFLKPKQGIP 295
Query: 463 CLRNFCQQLPQS*KSRTTY*LKRPKVTLVQIFVSCAKKLPCNH*DVSCQFLKPVTSWFRR 642
CLRNFC Q PQ *+S +*LKR K TLV+IFV CAK+L C+H* VSCQF KPV SW +R
Sbjct: 296 CLRNFCPQ*PQI*RSHMIF*LKRLKGTLVRIFVLCAKRLLCSH*GVSCQFSKPVMSWCQR 475
Query: 643 KNCLRLAL*NLMILNLH*GTLGRQHISMRIAMRSSTRTTVAMSF 774
K+CLRLAL NL NL * T GRQHIS I M++ TRTT A+ F
Sbjct: 476 KSCLRLALSNLTTWNLL*ATQGRQHISKHIGMKNLTRTTGAIFF 607
Score = 182 bits (393), Expect(2) = 3e-055
Identities = 86/128 (67%), Positives = 98/128 (76%)
Frame = +2 / +3
Query: 212 TIHNIP**NRCYHQPAW*SSQ*A*G*SSLED*ATYSDGWFDKDKRLGICTCSYKSSLGIG 391
TIHNI **+ CY+ AW*SS A* *S+LED* TYSDGW DK++RL IC CS KS++GIG
Sbjct: 45 TIHNIS**D*CYY*SAW*SS*RA*S*STLED*TTYSDGWLDKNQRLSICACSNKSTMGIG 224
Query: 392 CSHAPPA*EADSCTAS*R*SKASDV*GTSASNYLKVRNPVRHTS*KDRRLLWFRYSSRVQ 571
CS+AP A*EADSCT S* SKA V*GTSA N LK R P+ + S*KD R+LWF YSS VQ
Sbjct: 225 CSYAPSA*EADSCTTS*SRSKAFHV*GTSARNDLKSRGPI*YFS*KD*RVLWFGYSSCVQ 404
Query: 572 RSCHATTE 595
R C+A E
Sbjct: 405 RGCYAAIE 428
Score = 53.3 bits (110), Expect(2) = 3e-055
Identities = 24/39 (61%), Positives = 30/39 (76%)
Frame = +2 / +3
Query: 650 A*GWPSET**Y*TCIKEH*AVSTSPCASL*EVQPGLR*P 766
A*GWPS+T* TC ++H AVSTSP S+*++ PGLR P
Sbjct: 483 A*GWPSQT*RRGTCFEQHKAVSTSPSTSV*KI*PGLREP 599
Score = 29.9 bits (59), Expect(2) = 8e-065
Identities = 11/15 (73%), Positives = 12/15 (80%)
Frame = +1 / +2
Query: 181 KFCLSLLGIMHHPQY 225
+F LSL IMHHPQY
Sbjct: 14 RFFLSLQDIMHHPQY 58
>gb|BE060099.2|BE060099 HVSMEg0011H06f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011H06f, mRNA sequence
Length = 543
Score = 372 bits (807), Expect = e-102
Identities = 161/170 (94%), Positives = 164/170 (96%)
Frame = +3 / +2
Query: 81 LAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQR 260
LAKAVATEC TTFFNISASSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQR
Sbjct: 2 LAKAVATECNTTFFNISASSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQR 181
Query: 261 GEARSEHEASRRLKTELLIQMDGLTKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLP 440
GEARSEHEASRRLKTELLIQMDGLTKT DLVFVLAATNLPWELDAAMLRRLEKRILVPLP
Sbjct: 182 GEARSEHEASRRLKTELLIQMDGLTKTKDLVFVLAATNLPWELDAAMLRRLEKRILVPLP 361
Query: 441 EGEARQAMFEELLPATTSKLEIPYDILVEKTEGYSGSDIRLVCKEAAMQP 590
E EAR +MF+ELLPA TS LE+PYDILVEKTEGYSGSDIRLVCKEAAMQP
Sbjct: 362 EAEARHSMFKELLPAMTSNLEVPYDILVEKTEGYSGSDIRLVCKEAAMQP 511
Score = 329 bits (713), Expect = 2e-089
Identities = 149/171 (87%), Positives = 158/171 (92%)
Frame = -1 / -1
Query: 592 SGCMAASLHTRRISEPE*PSVFSTSMSYGISNFEVVAGRSSSNIACLASPSGSGTRIRFS 413
+GC+AASLHTRRISEPE PSVFST +SYG S FEV+AGRSS N+ CLAS SGSGTRIRFS
Sbjct: 513 NGCIAASLHTRRISEPEYPSVFSTKISYGTSRFEVIAGRSSLNMECLASASGSGTRIRFS 334
Query: 412 SRRSMAASNSQGRFVAASTNTKSFVFVKPSI*ISSSVFKRRLASCSLRASPRWLMIASIS 233
SRRS+AASNS GRFVAASTNTKS VFVKPSI*ISSSVFKRRLASCSLRASPR L+IASIS
Sbjct: 333 SRRSIAASNSHGRFVAASTNTKSLVFVKPSI*ISSSVFKRRLASCSLRASPR*LIIASIS 154
Query: 232 SRNIVDGA*CLASSNRTLTSFSESPRHLLTIDDAEMLKKVVLHSVATAFAS 80
SRNIVDGA*CLASS RTLTSFSESPRHLLTID+AEMLK VVLHSVATA A+
Sbjct: 153 SRNIVDGA*CLASSKRTLTSFSESPRHLLTIDEAEMLKNVVLHSVATALAN 1
Score = 277 bits (599), Expect = 9e-074
Identities = 119/171 (69%), Positives = 132/171 (77%)
Frame = -3 / -3
Query: 593 QWLHGSFFAHETNI*TRVTFGLFN*YVVRDF*L*GSCWQKFLKHRLPCFTFRKRYKNPLL 414
+WLH S FAH+TNI TRV F LFN* ++ D *+*G C QKFLKH +PCF+FRK YKNPLL
Sbjct: 514 KWLHSSLFAHKTNIRTRVPFSLFN*NIIWDL*I*GHCGQKFLKHGMPCFSFRKWYKNPLL 335
Query: 413 KPAEHGCIQFPGKICSCKYKYQVVCLCQTIHLNK*LSLQATTSLMLTASFTTLADDSIYF 234
KP EH CIQFP IC CK+KY V CQ IHLNK* SLQA+TS ML SFTTL ++SI
Sbjct: 334 KPTEHSCIQFPW*ICCCKHKY*VFGFCQAIHLNK*FSLQASTSFMLATSFTTLTNNSINL 155
Query: 233 IKEYCGWCMMPSKLKQNFDKLL*ISTPFADN**C*NVEEGGFALSSDRFCQ 81
IK+YCGWCMM KLK+NFD LL ISTPFADN** NVEE ALSSDRF Q
Sbjct: 154 IKKYCGWCMMSCKLKKNFD*LLRISTPFADN**SRNVEECSIALSSDRFGQ 2
Score = 235 bits (508), Expect = 3e-061
Identities = 109/168 (64%), Positives = 124/168 (73%)
Frame = +2 / +1
Query: 83 GKSGRY*VQNHLLQHFSIINCQQMAWRFREXXXXXXXXXXXXXTIHNIP**NRCYHQPAW 262
G+SGRY*VQ ++LQHF INCQQMAWRF E TIHNI **+ CY+ AW
Sbjct: 4 GQSGRY*VQYYILQHFGFINCQQMAWRF*EASQSSF*ACKTSCTIHNIS**D*CYY*SAW 183
Query: 263 *SSQ*A*G*SSLED*ATYSDGWFDKDKRLGICTCSYKSSLGIGCSHAPPA*EADSCTAS* 442
*SS A* *S+LED* TYSDGW DK++RL IC CS KS++GIGCS+AP A*EADSCT S*
Sbjct: 184 *SS*RA*S*STLED*TTYSDGWLDKNQRLSICACSNKSTMGIGCSYAPSA*EADSCTTS* 363
Query: 443 R*SKASDV*GTSASNYLKVRNPVRHTS*KDRRLLWFRYSSRVQRSCHA 586
*SKA V*GTSA N LK R P+ + S*KD R+LWF YSS VQR C+A
Sbjct: 364 S*SKAFHV*GTSARNDLKSRGPI*YFS*KD*RVLWFGYSSCVQRGCYA 507
Score = 228 bits (492), Expect = 5e-059
Identities = 118/170 (69%), Positives = 123/170 (72%)
Frame = -2 / -2
Query: 591 VVAWQLLCTRDEYLNQSNLRSFQLVCRTGFLTLR*LLAEVPQTSLALLHLQEAVQESASQ 412
+ A Q LCT+DEY NQS L+SFQL G L LR L AEVP T ALL LQE VQESASQ
Sbjct: 512 MAA*QPLCTQDEYPNQSTLQSFQLKYHMGPLDLRSLRAEVP*TWNALLQLQEVVQESASQ 333
Query: 411 AGGAWLHPIPREDL*LQVQIPSRLSLSNHPSE*VAQSSSDD*PHAHCELHHAG***HLFH 232
A GA LHPIP DL LQ QI S LS+HPSE*V QSSS D* HA ELHHA ***H H
Sbjct: 332 ADGA*LHPIPMVDLLLQAQILSLWFLSSHPSE*VVQSSSVD*LHARYELHHAD***HQSH 153
Query: 231 QGILWMVHDA*QAQTEL*QASLNLHAIC*QLMMLKC*RRWFCTQ*RPLLP 82
Q ILWMVHD QAQ EL* AS NLHAIC*QLM KC*R +CTQ*RPL P
Sbjct: 152 QEILWMVHDVLQAQKEL*LASQNLHAIC*QLMKPKC*RM*YCTQ*RPLWP 3
Score = 159 bits (341), Expect(2) = 5e-056
Identities = 70/103 (67%), Positives = 80/103 (77%)
Frame = +1 / +3
Query: 283 RLVVA*RLSYLFRWMV*QRQTTWYLYLQLQIFPGNWMQPCSAGLRSGFLYRFLKVKQGKR 462
+LV A*RL+YLFRWM *Q+ T YL LQ QI+ GNWMQ CS GLRSGFLY FLK+KQG
Sbjct: 204 KLVDA*RLNYLFRWMA*QKPKT*YLCLQQQIYHGNWMQLCSVGLRSGFLYHFLKLKQGIP 383
Query: 463 CLRNFCQQLPQS*KSRTTY*LKRPKVTLVQIFVSCAKKLPCNH 591
CLRNFC Q PQ *+S +*LKR K TLV+IFV CAK+L C+H
Sbjct: 384 CLRNFCPQ*PQI*RSHMIF*LKRLKGTLVRIFVLCAKRLLCSH 512
Score = 79.9 bits (168), Expect(2) = 5e-056
Identities = 37/48 (77%), Positives = 38/48 (79%)
Frame = +1 / +3
Query: 82 WQKRSLLSAKPPSSTFQHHQLSANGVEIQRSLSKFCLSLLGIMHHPQY 225
W KRSLLSA SSTF+ HQLSANGVEI RS SKF LSL IMHHPQY
Sbjct: 3 WPKRSLLSAILHSSTFRLHQLSANGVEILRS*SKFFLSLQDIMHHPQY 146
>gb|BF260284.3|BF260284 HVSMEf0021J12f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0021J12f, mRNA sequence
Length = 815
Score = 285 bits (616), Expect(3) = 2e-086
Identities = 124/137 (90%), Positives = 125/137 (91%)
Frame = +3 / +3
Query: 3 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVK 182
PKYFTGLLSP KGILLFGPPGTGKTMLAKAVATEC TTFFNISASSIV+KWRGDSEKLVK
Sbjct: 231 PKYFTGLLSPRKGILLFGPPGTGKTMLAKAVATECNTTFFNISASSIVTKWRGDSEKLVK 410
Query: 183 VLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASRRLKTELLIQMDGLTKTNDLVFVL 362
LFELARHHAP TIFLDEIDAII QRGEARSEHEASRRLKTELLIQMDGLTKT DLVF L
Sbjct: 411 GLFELARHHAPFTIFLDEIDAIIIQRGEARSEHEASRRLKTELLIQMDGLTKTKDLVFGL 590
Query: 363 AATNLPWELDAAMLRRL 413
A TNLPWELDA RL
Sbjct: 591 ATTNLPWELDAVCSVRL 641
Score = 254 bits (549), Expect(3) = 2e-075
Identities = 113/137 (82%), Positives = 121/137 (88%)
Frame = -1 / -2
Query: 412 SRRSMAASNSQGRFVAASTNTKSFVFVKPSI*ISSSVFKRRLASCSLRASPRWLMIASIS 233
SR ASNS GR V AS NTKS VFVKPSI*ISSSVFKRRLASCSLRASPR ++IASIS
Sbjct: 640 SRTEHTASNSHGRLVVASPNTKSLVFVKPSI*ISSSVFKRRLASCSLRASPR*IIIASIS 461
Query: 232 SRNIVDGA*CLASSNRTLTSFSESPRHLLTIDDAEMLKKVVLHSVATAFASIVFPVPGGP 53
SRNIV+GA*CLASS R LTSFSESPRHL+TID+AEMLK VVLHSVATA A++VFPVPGGP
Sbjct: 460 SRNIVNGA*CLASSKRPLTSFSESPRHLVTIDEAEMLKNVVLHSVATALANMVFPVPGGP 281
Query: 52 KSKMPFHGERRPVKYFG 2
KSKMPF G+ RPVKYFG
Sbjct: 280 KSKMPFRGDSRPVKYFG 230
Score = 185 bits (399), Expect(2) = 1e-051
Identities = 80/137 (58%), Positives = 94/137 (68%)
Frame = +2 / +2
Query: 2 PKILHWSPFSMERHLTFWPPRNRKDNAGKSGRY*VQNHLLQHFSIINCQQMAWRFREXXX 181
PKILHWS + ERHLTFW RNR+D+ G+SGRY*VQ ++LQHF INC QMAWRF E
Sbjct: 230 PKILHWSTITAERHLTFWSSRNREDHVGQSGRY*VQYYILQHFRFINCHQMAWRF*EASQ 409
Query: 182 XXXXXXXXXXTIHNIP**NRCYHQPAW*SSQ*A*G*SSLED*ATYSDGWFDKDKRLGICT 361
TIHNI **+ CY+ W*SS A* *S+LED* TYSDGW DK++RL I
Sbjct: 410 RSF*ACKTSCTIHNIS**D*CYYYSTW*SS*RA*S*STLED*TTYSDGWLDKNQRLSIWA 589
Query: 362 CSYKSSLGIGCSHAPPA 412
C+ S++GIGC PA
Sbjct: 590 CNN*STMGIGCRMLRPA 640
Score = 183 bits (395), Expect(2) = 1e-048
Identities = 87/130 (66%), Positives = 92/130 (70%)
Frame = -3 / -1
Query: 392 IQFPGKICSCKYKYQVVCLCQTIHLNK*LSLQATTSLMLTASFTTLADDSIYFIKEYCGW 213
IQFP I CK KY V CQ IHLNK* SLQA+TS ML SFTTL ++SI IK+YC W
Sbjct: 620 IQFPW*ISCCKPKY*VFGFCQAIHLNK*FSLQASTSFMLATSFTTLNNNSINLIKKYCEW 441
Query: 212 CMMPSKLKQNFDKLL*ISTPFADN**C*NVEEGGFALSSDRFCQHCLSCSWGAKK*DAFP 33
CMM KLK+ FD LL ISTPF DN** NVEE ALSSDRF QH L CSW KK*DAFP
Sbjct: 440 CMMSCKLKKTFD*LLRISTPFGDN**SGNVEECSIALSSDRFGQHGLPCSWRTKK*DAFP 261
Query: 32 WRKETSEVFW 3
TSEVFW
Sbjct: 260 R***TSEVFW 231
Score = 165 bits (355), Expect = 4e-040
Identities = 90/137 (65%), Positives = 94/137 (68%)
Frame = -2 / -3
Query: 411 AGGAWLHPIPREDL*LQVQIPSRLSLSNHPSE*VAQSSSDD*PHAHCELHHAG***HLFH 232
AG + HPIP D LQ QI S LS+HPSE*V QSSS D* HA ELHH ***H H
Sbjct: 639 AGRSIRHPIPMVD*LLQAQILSLWFLSSHPSE*VVQSSSVD*LHARYELHHVE***HQSH 460
Query: 231 QGILWMVHDA*QAQTEL*QASLNLHAIC*QLMMLKC*RRWFCTQ*RPLLPALSFLFLGGQ 52
Q IL MVHD QAQ +L* AS NLHAI *QLM KC*R +CTQ*RPL P S LFL Q
Sbjct: 459 QEIL*MVHDVLQAQKDL*LASQNLHAIW*QLMKRKC*RM*YCTQ*RPLWPTWSSLFLEDQ 280
Query: 51 KVRCLSMEKGDQ*SILG 1
KVRCLS DQ*SILG
Sbjct: 279 KVRCLSAVIVDQ*SILG 229
Score = 111 bits (236), Expect(4) = 5e-035
Identities = 53/75 (70%), Positives = 54/75 (72%)
Frame = +1 / +1
Query: 1 TQNTSLVSFLHGKASYFLAPQEQERQCWQKRSLLSAKPPSSTFQHHQLSANGVEIQRSLS 180
TQNTSLV + GKASYFL QEQ R CW KRSLLSA SSTF HQLS NGVEI RS S
Sbjct: 229 TQNTSLVYYHRGKASYFLVLQEQGRPCWPKRSLLSAILHSSTFPLHQLSPNGVEILRS*S 408
Query: 181 KFCLSLLGIMHHPQY 225
K LSL IMHH QY
Sbjct: 409 KVFLSLQDIMHHSQY 453
Score = 55.6 bits (115), Expect(4) = 5e-035
Identities = 25/43 (58%), Positives = 30/43 (69%)
Frame = +1 / +1
Query: 283 RLVVA*RLSYLFRWMV*QRQTTWYLYLQLQIFPGNWMQPCSAG 411
+LV A*RL+YLFRWM *Q+ T YL LQ I+ GNWM +G
Sbjct: 511 KLVDA*RLNYLFRWMA*QKPKT*YLGLQQLIYHGNWMPYAPSG 639
Score = 41.4 bits (84), Expect(3) = 2e-075
Identities = 14/19 (73%), Positives = 16/19 (84%)
Frame = -3 / -3
Query: 491 GSCWQKFLKHRLPCFTFRK 435
G C QKFLKH +PCF+FRK
Sbjct: 717 GPCGQKFLKHGMPCFSFRK 661
Score = 38.6 bits (78), Expect(2) = 1e-051
Identities = 20/47 (42%), Positives = 28/47 (59%)
Frame = +3 / +1
Query: 432 PLPEGEARQAMFEELLPATTSKLEIPYDILVEKTEGYSGSDIRLVCK 572
PL E EAR +MF+ELLPA T + P + ++ +G +IRL K
Sbjct: 658 PLSEAEARHSMFKELLPAWT*I*KAPMNFYWKRLKGTLVRNIRLGAK 798
Score = 37.7 bits (76), Expect(3) = 2e-086
Identities = 15/24 (62%), Positives = 17/24 (70%)
Frame = +1 / +2
Query: 427 LYRFLKVKQGKRCLRNFCQQLPQS 498
L FLK+KQG CLRNFC P+S
Sbjct: 653 LVHFLKLKQGIPCLRNFCPHGPKS 724
Score = 36.8 bits (74), Expect(3) = 2e-086
Identities = 16/29 (55%), Positives = 21/29 (72%)
Frame = +3 / +3
Query: 498 LEIPYDILVEKTEGYSGSDIRLVCKEAAM 584
LE PY+ L+EKT GYSGS+ L +EA +
Sbjct: 723 LEGPYEFLLEKT*GYSGSEYSLGGQEAVL 809
Score = 30.4 bits (60), Expect(2) = 1e-048
Identities = 14/20 (70%), Positives = 15/20 (75%)
Frame = -1 / -1
Query: 490 VVAGRSSSNIACLASPSGSG 431
V AGRSS N+ CLAS S SG
Sbjct: 716 VHAGRSSLNMECLASASESG 657
Score = 27.2 bits (53), Expect(3) = 2e-075
Identities = 15/31 (48%), Positives = 15/31 (48%)
Frame = -1 / -2
Query: 586 CMAASLHTRRISEPE*PSVFSTSMSYGISNF 494
C AS SEPE P VFS S G S F
Sbjct: 811 CNTASWPPSEYSEPEYP*VFSNKNS*GPSRF 719
Score = 21.7 bits (41), Expect(4) = 5e-035
Identities = 12/31 (38%), Positives = 15/31 (48%)
Frame = +2 / +2
Query: 494 KVRNPVRHTS*KDRRLLWFRYSSRVQRSCHA 586
K R P+ KD R+LWF + R C A
Sbjct: 719 KSRRPL*IFIGKDLRVLWFGIFAWGPRGCIA 811
Score = 21.7 bits (41), Expect(4) = 5e-035
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = +2 / +3
Query: 446 *SKASDV*GTSASNYLKVRNP 508
*SKA V*GTSA L + P
Sbjct: 672 *SKAFHV*GTSARMDLNLEGP 734
>gb|BF254000.2|BF254000 HVSMEf0002L12f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0002L12f, mRNA sequence
Length = 852
Score = 274 bits (592), Expect = 8e-073
Identities = 117/144 (81%), Positives = 122/144 (84%)
Frame = +3 / +1
Query: 3 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVK 182
PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATEC TTF NISASSIV+KWRGDSEKLVK
Sbjct: 421 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECNTTFLNISASSIVNKWRGDSEKLVK 600
Query: 183 VLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASRRLKTELLIQMDGLTKTNDLVFVL 362
VLFELARH+AP+TIFLDEIDAIISQRGEAR+EHEASRRLK ELLIQMDG K L+F L
Sbjct: 601 VLFELARHYAPTTIFLDEIDAIISQRGEARAEHEASRRLKNELLIQMDGFAKNQGLIFGL 780
Query: 363 AATNLPWELDAAMLRRLEKRILVP 434
AATNLPWE L L P
Sbjct: 781 AATNLPWEWMQVCFVGLRNGFLAP 852
Score = 237 bits (511), Expect(2) = 3e-064
Identities = 105/129 (81%), Positives = 112/129 (86%)
Frame = -1 / -2
Query: 388 NSQGRFVAASTNTKSFVFVKPSI*ISSSVFKRRLASCSLRASPRWLMIASISSRNIVDGA 209
+S GRFVAAS N + KPSI*ISSS FKRRLASCS RASPR L+IASISSRNIV GA
Sbjct: 806 HSHGRFVAASPNISPWFLAKPSI*ISSSFFKRRLASCSARASPR*LIIASISSRNIVVGA 627
Query: 208 *CLASSNRTLTSFSESPRHLLTIDDAEMLKKVVLHSVATAFASIVFPVPGGPKSKMPFHG 29
*CLASS RTLTSFSESPRHLLTID+AEM K VVLHSVATA A++VFPVPGGPKSKMPFHG
Sbjct: 626 *CLASSKRTLTSFSESPRHLLTIDEAEMFKNVVLHSVATALANMVFPVPGGPKSKMPFHG 447
Query: 28 ERRPVKYFG 2
+ RPVKYFG
Sbjct: 446 DSRPVKYFG 420
Score = 181 bits (389), Expect(2) = 1e-047
Identities = 82/128 (64%), Positives = 93/128 (72%)
Frame = -3 / -1
Query: 386 FPGKICSCKYKYQVVCLCQTIHLNK*LSLQATTSLMLTASFTTLADDSIYFIKEYCGWCM 207
FP +IC CK KY+ + + IHLNK* LQA+TS ML SFTTL ++SI IK+YCGWC+
Sbjct: 804 FPWEICCCKPKYKSLVFGKAIHLNK*FILQASTSFMLGTSFTTLTNNSINLIKKYCGWCI 625
Query: 206 MPSKLKQNFDKLL*ISTPFADN**C*NVEEGGFALSSDRFCQHCLSCSWGAKK*DAFPWR 27
M KLK+NFD LL ISTPF DN** NV+E ALSSDRF QH L CSW KK*DAFP
Sbjct: 624 MSCKLKKNFD*LLRISTPFVDN**SRNVQECSIALSSDRFGQHGLPCSWRTKK*DAFPR* 445
Query: 26 KETSEVFW 3
TSEVFW
Sbjct: 444 **TSEVFW 421
Score = 164 bits (353), Expect(2) = 1e-043
Identities = 72/129 (55%), Positives = 87/129 (67%)
Frame = +2 / +3
Query: 2 PKILHWSPFSMERHLTFWPPRNRKDNAGKSGRY*VQNHLLQHFSIINCQQMAWRFREXXX 181
PKILHWS ++ERHLTFW RNR+D+ G+SGRY*VQ ++L+HF INCQQMAWRF E
Sbjct: 420 PKILHWSTITVERHLTFWSSRNREDHVGQSGRY*VQYYILEHFGFINCQQMAWRF*EASQ 599
Query: 182 XXXXXXXXXXTIHNIP**NRCYHQPAW*SSQ*A*G*SSLED*ATYSDGWFDKDKRLGICT 361
T HNI **+ CY+ W*SS A* *S+LE+* TYSDGW + R I
Sbjct: 600 SSF*ACKTLCTNHNIS**D*CYY*STW*SSCRA*S*STLEE*TTYSDGWLCQKPRTYIWA 779
Query: 362 CSYKSSLGI 388
CS KS +G+
Sbjct: 780 CSNKSPMGM 806
Score = 149 bits (319), Expect(2) = 7e-038
Identities = 75/110 (68%), Positives = 79/110 (71%)
Frame = -2 / -3
Query: 330 NHPSE*VAQSSSDD*PHAHCELHHAG***HLFHQGILWMVHDA*QAQTEL*QASLNLHAI 151
+HPSE*V SSS D* HA ELHH ***H HQ ILW+VH+ QAQ EL* AS NLHAI
Sbjct: 748 SHPSE*VVHSSSVD*LHARHELHHVD***HQSHQEILWLVHNVLQAQKEL*LASQNLHAI 569
Query: 150 C*QLMMLKC*RRWFCTQ*RPLLPALSFLFLGGQKVRCLSMEKGDQ*SILG 1
C*QLM KC R +CTQ*RPL P S LFL QKVRCLS DQ*SILG
Sbjct: 568 C*QLMKPKCSRM*YCTQ*RPLWPTWSSLFLEDQKVRCLSTVIVDQ*SILG 419
Score = 111 bits (238), Expect(3) = 2e-034
Identities = 53/75 (70%), Positives = 55/75 (73%)
Frame = +1 / +2
Query: 1 TQNTSLVSFLHGKASYFLAPQEQERQCWQKRSLLSAKPPSSTFQHHQLSANGVEIQRSLS 180
TQNTSLV + GKASYFL QEQ R CW KRSLLSA S TF+ HQLS NGVEI RS S
Sbjct: 419 TQNTSLVYYHRGKASYFLVLQEQGRPCWPKRSLLSAILHS*TFRLHQLSTNGVEILRS*S 598
Query: 181 KFCLSLLGIMHHPQY 225
KF LSL IMH PQY
Sbjct: 599 KFFLSLQDIMHQPQY 643
Score = 40.5 bits (82), Expect(3) = 2e-034
Identities = 19/35 (54%), Positives = 23/35 (65%)
Frame = +1 / +2
Query: 283 RLVVA*RLSYLFRWMV*QRQTTWYLYLQLQIFPGN 387
+LV A*R++YLFRWM + YL LQ QI GN
Sbjct: 701 KLVDA*RMNYLFRWMALPKTKDLYLGLQQQISHGN 805
Score = 33.1 bits (66), Expect(2) = 1e-043
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = +3 / +3
Query: 378 PWELDAAMLRRLEKRILVP 434
P +DA MLRRLEKRIL P
Sbjct: 795 PMGMDAGMLRRLEKRILGP 851
Score = 31.8 bits (63), Expect(3) = 2e-034
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = +1 / +1
Query: 379 PGNWMQPCSAGLRSGFL 429
P WMQ C GLR+GFL
Sbjct: 796 PWEWMQVCFVGLRNGFL 846
Score = 29.5 bits (58), Expect(2) = 1e-047
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = -2 / -1
Query: 429 QESASQAGGAWLHPIPRE 376
QES SQA A+LHP P E
Sbjct: 846 QESVSQADEAYLHPFPWE 793
Score = 29.5 bits (58), Expect(2) = 3e-064
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -3 / -2
Query: 428 KNPLLKPAEHGCIQFPGK 375
KNP LKP +H CI G+
Sbjct: 845 KNPFLKPTKHTCIHSHGR 792
Score = 29.0 bits (57), Expect(2) = 7e-038
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = -1 / -3
Query: 433 GTRIRFSSRRSMAASNSQG 377
G RIRFSSRRS+ AS G
Sbjct: 850 GPRIRFSSRRSIPASIPMG 794
>gb|AV834569.1|AV834569 AV834569 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags3f23, mRNA sequence
Length = 643
Score = 241 bits (520), Expect = 7e-063
Identities = 102/131 (77%), Positives = 106/131 (80%)
Frame = +3 / -1
Query: 378 PWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIPYDILVEKTEGYSGSDI 557
PWELDAAMLRRLEKRILVPLPE EAR AMFEE LP T +EIPYD LVE TEGYSGSDI
Sbjct: 643 PWELDAAMLRRLEKRILVPLPEAEARHAMFEEFLPLTQDTMEIPYDALVENTEGYSGSDI 464
Query: 558 RLVCKEAAMQPLRRLMSVLEASDXXXXXXXXXXXGPLKPDDIELALRNTRPSAHLHAHRY 737
RLVCKEAAMQPLRRLM+VLE GP+ +DIELALRNTRPSAHLHAHRY
Sbjct: 463 RLVCKEAAMQPLRRLMAVLEGRQEEIPEDELPEVGPIATEDIELALRNTRPSAHLHAHRY 284
Query: 738 EKFNQDYGSHV 770
EKFNQDYGSHV
Sbjct: 283 EKFNQDYGSHV 251
Score = 169 bits (363), Expect = 3e-041
Identities = 82/129 (63%), Positives = 89/129 (68%)
Frame = -1 / +3
Query: 769 TWLP*SWLNFS*RCAWRCADGLVFLNASSIXXXXXXXXXXXXXXXXXXXXXSRTDMRRLS 590
TWLP*S LNFS CAWR ADG VFL ASSI SRT + RLS
Sbjct: 252 TWLP*S*LNFSYLCAWR*ADGRVFLRASSISSVAIGPTSGNSSSGISSCLPSRTAINRLS 431
Query: 589 GCMAASLHTRRISEPE*PSVFSTSMSYGISNFEVVAGRSSSNIACLASPSGSGTRIRFSS 410
GC+AASL TRRISEPE PSVFST SYGIS V GR+SSNIACLAS SG GTRIRFS+
Sbjct: 432 GCIAASLQTRRISEPEYPSVFSTRASYGISIVS*VNGRNSSNIACLASASGKGTRIRFSN 611
Query: 409 RRSMAASNS 383
RR++AAS+S
Sbjct: 612 RRNIAASSS 638
>gb|CV054720.1|CV054720 BNEL112e7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL112e7 5' similar to
AAA-ATPase-like protein, mRNA sequence
Length = 702
Score = 142 bits (305), Expect(3) = 5e-059
Identities = 56/97 (57%), Positives = 73/97 (75%)
Frame = +3 / +1
Query: 3 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVK 182
P++FTG PW+ LL+GPPGTGK+ LAKAVATE +TFF+IS+S +VSKW G+SEKLV
Sbjct: 112 PQFFTGKRRPWRAFLLYGPPGTGKSYLAKAVATEADSTFFSISSSDLVSKWMGESEKLVA 291
Query: 183 VLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASR 293
LF++AR +APS IF+DEID++ RGE + R
Sbjct: 292 NLFQMARENAPSIIFIDEIDSLCGTRGEGNESEASRR 402
Score = 94.5 bits (200), Expect(3) = 5e-059
Identities = 42/76 (55%), Positives = 54/76 (71%)
Frame = +3 / +1
Query: 273 SEHEASRRLKTELLIQMDGLTKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEA 452
+E EASRR+KTELL+QM G+ +D V VLAATN P+ LD A+ RR +KRI +PLP+ +A
Sbjct: 379 NESEASRRIKTELLVQMQGVGHNDDKVLVLAATNTPYALDQAVRRRFDKRIYIPLPDAKA 558
Query: 453 RQAMFEELLPATTSKL 500
RQ MF+ L T L
Sbjct: 559 RQHMFKVHLGDTPHSL 606
Score = 83.5 bits (176), Expect(2) = 2e-027
Identities = 45/88 (51%), Positives = 50/88 (56%)
Frame = -1 / -2
Query: 265 SPRWLMIASISSRNIVDGA*CLASSNRTLTSFSESPRHLLTIDDAEMLKKVVLHSVATAF 86
SPR SISS I++GA A R TSFS SP H T D +MLK V SVATA
Sbjct: 374 SPRVPHKESISSMKIIEGAFSRAIWKRLATSFSLSPIHFETRSDEDMLKNVESASVATAL 195
Query: 85 ASIVFPVPGGPKSKMPFHGERRPVKYFG 2
A FPVPGGP + HG R PVK +G
Sbjct: 194 AK*DFPVPGGPYRRKALHGLRLPVKNWG 111
Score = 59.3 bits (123), Expect(2) = 2e-027
Identities = 35/72 (48%), Positives = 41/72 (56%)
Frame = -1 / -2
Query: 487 VAGRSSSNIACLASPSGSGTRIRFSSRRSMAASNSQGRFVAASTNTKSFVFVKPSI*ISS 308
V+ R + NI CLA SG G IR S+RR A S + G FVAA T T S ++ P I S
Sbjct: 593 VSPRCTLNICCLAFASGRGM*IRLSNRRRTAWSRAYGVFVAARTRTLSSLWPTPCICTRS 414
Query: 307 SVFKRRLASCSL 272
SVF AS SL
Sbjct: 413 SVFILLEASLSL 378
Score = 30.4 bits (60), Expect(3) = 5e-059
Identities = 10/31 (32%), Positives = 19/31 (61%)
Frame = +3 / +1
Query: 501 EIPYDILVEKTEGYSGSDIRLVCKEAAMQPL 593
E ++L +T G+SGSD+ + K+ +P+
Sbjct: 610 ESDXEVLGRRTRGFSGSDVAVCVKDVLFEPV 702
>gb|CV063832.1|CV063832 BNEL93f7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL93f7 5' similar to
AAA-ATPase-like protein, mRNA sequence
Length = 703
Score = 142 bits (305), Expect(3) = 3e-058
Identities = 56/97 (57%), Positives = 73/97 (75%)
Frame = +3 / +1
Query: 3 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVK 182
P++FTG PW+ LL+GPPGTGK+ LAKAVATE +TFF+IS+S +VSKW G+SEKLV
Sbjct: 112 PQFFTGKRRPWRAFLLYGPPGTGKSYLAKAVATEADSTFFSISSSDLVSKWMGESEKLVA 291
Query: 183 VLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASR 293
LF++AR +APS IF+DEID++ RGE + R
Sbjct: 292 NLFQMARENAPSIIFIDEIDSLCGTRGEGNESEASRR 402
Score = 93.6 bits (198), Expect(3) = 3e-058
Identities = 39/66 (59%), Positives = 51/66 (77%)
Frame = +3 / +1
Query: 273 SEHEASRRLKTELLIQMDGLTKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEA 452
+E EASRR+KTELL+QM G+ +D V VLAATN P+ LD A+ RR +KRI +PLP+ +A
Sbjct: 379 NESEASRRIKTELLVQMQGVGHNDDKVLVLAATNTPYALDQAVRRRFDKRIYIPLPDAKA 558
Query: 453 RQAMFE 470
RQ MF+
Sbjct: 559 RQHMFK 576
Score = 83.5 bits (176), Expect(2) = 2e-027
Identities = 45/88 (51%), Positives = 50/88 (56%)
Frame = -1 / -3
Query: 265 SPRWLMIASISSRNIVDGA*CLASSNRTLTSFSESPRHLLTIDDAEMLKKVVLHSVATAF 86
SPR SISS I++GA A R TSFS SP H T D +MLK V SVATA
Sbjct: 374 SPRVPHKESISSMKIIEGAFSRAIWKRLATSFSLSPIHFETRSDEDMLKNVESASVATAL 195
Query: 85 ASIVFPVPGGPKSKMPFHGERRPVKYFG 2
A FPVPGGP + HG R PVK +G
Sbjct: 194 AK*DFPVPGGPYRRKALHGLRLPVKNWG 111
Score = 59.3 bits (123), Expect(2) = 2e-027
Identities = 35/72 (48%), Positives = 41/72 (56%)
Frame = -1 / -3
Query: 487 VAGRSSSNIACLASPSGSGTRIRFSSRRSMAASNSQGRFVAASTNTKSFVFVKPSI*ISS 308
V+ R + NI CLA SG G IR S+RR A S + G FVAA T T S ++ P I S
Sbjct: 593 VSPRCTLNICCLAFASGRGM*IRLSNRRRTAWSRAYGVFVAARTRTLSSLWPTPCICTRS 414
Query: 307 SVFKRRLASCSL 272
SVF AS SL
Sbjct: 413 SVFILLEASLSL 378
Score = 28.6 bits (56), Expect(3) = 3e-058
Identities = 9/28 (32%), Positives = 19/28 (67%)
Frame = +3 / +1
Query: 510 YDILVEKTEGYSGSDIRLVCKEAAMQPL 593
+++L +TEG+S SD+ + K+ +P+
Sbjct: 619 FEVLGRRTEGFSVSDVAVCGKDVLFEPV 702
>gb|AV916868.1|AV916868 AV916868 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19o15 5', mRNA sequence
Length = 590
Score = 142 bits (305), Expect(2) = 2e-055
Identities = 56/97 (57%), Positives = 73/97 (75%)
Frame = +3 / +2
Query: 3 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVK 182
P++FTG PW+ LL+GPPGTGK+ LAKAVATE +TFF+IS+S +VSKW G+SEKLV
Sbjct: 59 PQFFTGKRRPWRAFLLYGPPGTGKSYLAKAVATEADSTFFSISSSDLVSKWMGESEKLVA 238
Query: 183 VLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASR 293
LF++AR +APS IF+DEID++ RGE + R
Sbjct: 239 NLFQMARENAPSIIFIDEIDSLCGTRGEGNESEASRR 349
Score = 94.5 bits (200), Expect(2) = 2e-055
Identities = 42/76 (55%), Positives = 54/76 (71%)
Frame = +3 / +2
Query: 273 SEHEASRRLKTELLIQMDGLTKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEA 452
+E EASRR+KTELL+QM G+ +D V VLAATN P+ LD A+ RR +KRI +PLP+ +A
Sbjct: 326 NESEASRRIKTELLVQMQGVGHNDDKVLVLAATNTPYALDQAVRRRFDKRIYIPLPDAKA 505
Query: 453 RQAMFEELLPATTSKL 500
RQ MF+ L T L
Sbjct: 506 RQHMFKVHLGDTPHSL 553
Score = 83.5 bits (176), Expect(2) = 2e-027
Identities = 45/88 (51%), Positives = 50/88 (56%)
Frame = -1 / -3
Query: 265 SPRWLMIASISSRNIVDGA*CLASSNRTLTSFSESPRHLLTIDDAEMLKKVVLHSVATAF 86
SPR SISS I++GA A R TSFS SP H T D +MLK V SVATA
Sbjct: 321 SPRVPHKESISSMKIIEGAFSRAIWKRLATSFSLSPIHFETRSDEDMLKNVESASVATAL 142
Query: 85 ASIVFPVPGGPKSKMPFHGERRPVKYFG 2
A FPVPGGP + HG R PVK +G
Sbjct: 141 AK*DFPVPGGPYRRKALHGLRLPVKNWG 58
Score = 59.3 bits (123), Expect(2) = 2e-027
Identities = 35/72 (48%), Positives = 41/72 (56%)
Frame = -1 / -3
Query: 487 VAGRSSSNIACLASPSGSGTRIRFSSRRSMAASNSQGRFVAASTNTKSFVFVKPSI*ISS 308
V+ R + NI CLA SG G IR S+RR A S + G FVAA T T S ++ P I S
Sbjct: 540 VSPRCTLNICCLAFASGRGM*IRLSNRRRTAWSRAYGVFVAARTRTLSSLWPTPCICTRS 361
Query: 307 SVFKRRLASCSL 272
SVF AS SL
Sbjct: 360 SVFILLEASLSL 325
>gb|CK124077.1|CK124077 BES1824106o13 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010O136 5-PRIME, mRNA sequence
Length = 752
Score = 114 bits (243), Expect(3) = 2e-054
Identities = 46/47 (97%), Positives = 46/47 (97%)
Frame = +3 / +2
Query: 3 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSI 143
PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATEC TTFFNISASSI
Sbjct: 434 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECNTTFFNISASSI 574
Score = 101 bits (216), Expect(3) = 8e-047
Identities = 40/47 (85%), Positives = 44/47 (93%)
Frame = -1 / -3
Query: 142 IDDAEMLKKVVLHSVATAFASIVFPVPGGPKSKMPFHGERRPVKYFG 2
ID+AEMLK VVLHSVATA A++VFPVPGGPKSKMPFHG+ RPVKYFG
Sbjct: 573 IDEAEMLKNVVLHSVATALANMVFPVPGGPKSKMPFHGDSRPVKYFG 433
Score = 95.9 bits (203), Expect(3) = 2e-054
Identities = 40/40 (100%), Positives = 40/40 (100%)
Frame = +3 / +3
Query: 144 VSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQRG 263
VSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQRG
Sbjct: 576 VSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQRG 695
Score = 87.2 bits (184), Expect(3) = 1e-027
Identities = 32/47 (68%), Positives = 39/47 (82%)
Frame = +2 / +1
Query: 2 PKILHWSPFSMERHLTFWPPRNRKDNAGKSGRY*VQNHLLQHFSIIN 142
PKILHWS ++ERHLTFW RNR+D+ G+SGRY*VQ ++LQHF IN
Sbjct: 433 PKILHWSTITVERHLTFWSSRNREDHVGQSGRY*VQYYILQHFGFIN 573
Score = 84.0 bits (177), Expect(3) = 8e-047
Identities = 38/41 (92%), Positives = 39/41 (95%)
Frame = -1 / -2
Query: 265 SPRWLMIASISSRNIVDGA*CLASSNRTLTSFSESPRHLLT 143
SPR L+IASISSRNIVDGA*CLASS RTLTSFSESPRHLLT
Sbjct: 697 SPR*LIIASISSRNIVDGA*CLASSKRTLTSFSESPRHLLT 575
Score = 67.5 bits (141), Expect(3) = 2e-027
Identities = 27/38 (71%), Positives = 31/38 (81%)
Frame = -3 / -1
Query: 257 LADDSIYFIKEYCGWCMMPSKLKQNFDKLL*ISTPFAD 144
L ++SI IK+YCGWCMM KLK+NFD LL ISTPFAD
Sbjct: 689 LTNNSINLIKKYCGWCMMSCKLKKNFD*LLRISTPFAD 576
Score = 66.6 bits (139), Expect(3) = 2e-027
Identities = 33/47 (70%), Positives = 33/47 (70%)
Frame = -3 / -2
Query: 143 N**C*NVEEGGFALSSDRFCQHCLSCSWGAKK*DAFPWRKETSEVFW 3
N** NVEE ALSSDRF QH L CSW KK*DAFP TSEVFW
Sbjct: 574 N**SRNVEECSIALSSDRFGQHGLPCSWRTKK*DAFPR***TSEVFW 434
Score = 63.8 bits (133), Expect(3) = 2e-024
Identities = 32/48 (66%), Positives = 34/48 (70%)
Frame = -2 / -1
Query: 144 QLMMLKC*RRWFCTQ*RPLLPALSFLFLGGQKVRCLSMEKGDQ*SILG 1
+LM KC*R +CTQ*RPL P S LFL QKVRCLS DQ*SILG
Sbjct: 575 KLMKPKC*RM*YCTQ*RPLWPTWSSLFLEDQKVRCLSTVIVDQ*SILG 432
Score = 62.0 bits (129), Expect(3) = 2e-024
Identities = 30/40 (75%), Positives = 31/40 (77%)
Frame = -2 / -3
Query: 258 AG***HLFHQGILWMVHDA*QAQTEL*QASLNLHAIC*QL 139
A ***H HQ ILWMVHD QAQ EL* AS NLHAIC*Q+
Sbjct: 690 AD***HQSHQEILWMVHDVLQAQKEL*LASQNLHAIC*QI 571
Score = 51.5 bits (106), Expect(3) = 1e-027
Identities = 23/28 (82%), Positives = 23/28 (82%)
Frame = +1 / +1
Query: 142 LSANGVEIQRSLSKFCLSLLGIMHHPQY 225
LSANGVEI RS SKF LSL IMHHPQY
Sbjct: 574 LSANGVEILRS*SKFFLSLQDIMHHPQY 657
Score = 41.8 bits (85), Expect(3) = 2e-054
Identities = 18/20 (90%), Positives = 18/20 (90%)
Frame = +3 / +2
Query: 261 GEARSEHEASRRLKTELLIQ 320
G RSEHEASRRLKTELLIQ
Sbjct: 692 GGXRSEHEASRRLKTELLIQ 751
Score = 40.5 bits (82), Expect(3) = 8e-047
Identities = 18/20 (90%), Positives = 18/20 (90%)
Frame = -1 / -3
Query: 319 *ISSSVFKRRLASCSLRASP 260
*ISSSVFKRRLASCSLR P
Sbjct: 750 *ISSSVFKRRLASCSLRXPP 691
Score = 26.7 bits (52), Expect(3) = 2e-027
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = -3 / -2
Query: 320 LNK*LSLQATTSLML 276
LNK* SLQA+TS ML
Sbjct: 751 LNK*FSLQASTSFML 707
Score = 24.4 bits (47), Expect(3) = 2e-024
Identities = 13/18 (72%), Positives = 13/18 (72%)
Frame = -2 / -1
Query: 321 SE*VAQSSSDD*PHAHCE 268
SE*V QSSS D* HA E
Sbjct: 752 SE*VVQSSSVD*LHARYE 699
Score = 23.1 bits (44), Expect(3) = 1e-027
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +1 / +3
Query: 283 RLVVA*RLSYLFR 321
+LV A*RL+YLFR
Sbjct: 714 KLVDA*RLNYLFR 752
>gb|CA011697.1|CA011697 HT03E21r HT Hordeum vulgare subsp. vulgare cDNA clone HT03E21
5-PRIME, mRNA sequence
Length = 582
Score = 142 bits (305), Expect(2) = 7e-054
Identities = 56/97 (57%), Positives = 73/97 (75%)
Frame = +3 / +2
Query: 3 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVK 182
P++FTG PW+ LL+GPPGTGK+ LAKAVATE +TFF+IS+S +VSKW G+SEKLV
Sbjct: 125 PQFFTGKRRPWRAFLLYGPPGTGKSYLAKAVATEADSTFFSISSSDLVSKWMGESEKLVA 304
Query: 183 VLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASR 293
LF++AR +APS IF+DEID++ RGE + R
Sbjct: 305 NLFQMARENAPSIIFIDEIDSLCGTRGEGNESEASRR 415
Score = 89.0 bits (188), Expect(2) = 7e-054
Identities = 37/62 (59%), Positives = 48/62 (77%)
Frame = +3 / +2
Query: 273 SEHEASRRLKTELLIQMDGLTKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEA 452
+E EASRR+KTELL+QM G+ +D V VLAATN P+ LD A+ RR +KRI +PLP+ +A
Sbjct: 392 NESEASRRIKTELLVQMQGVGHNDDKVLVLAATNTPYALDQAVRRRFDKRIYIPLPDAKA 571
Query: 453 RQ 458
RQ
Sbjct: 572 RQ 577
Score = 83.5 bits (176), Expect(2) = 1e-025
Identities = 45/88 (51%), Positives = 50/88 (56%)
Frame = -1 / -1
Query: 265 SPRWLMIASISSRNIVDGA*CLASSNRTLTSFSESPRHLLTIDDAEMLKKVVLHSVATAF 86
SPR SISS I++GA A R TSFS SP H T D +MLK V SVATA
Sbjct: 387 SPRVPHKESISSMKIIEGAFSRAIWKRLATSFSLSPIHFETRSDEDMLKNVESASVATAL 208
Query: 85 ASIVFPVPGGPKSKMPFHGERRPVKYFG 2
A FPVPGGP + HG R PVK +G
Sbjct: 207 AK*DFPVPGGPYRRKALHGLRLPVKNWG 124
Score = 53.8 bits (111), Expect(2) = 1e-025
Identities = 32/64 (50%), Positives = 36/64 (56%)
Frame = -1 / -1
Query: 463 IACLASPSGSGTRIRFSSRRSMAASNSQGRFVAASTNTKSFVFVKPSI*ISSSVFKRRLA 284
I CLA SG G IR S+RR A S + G FVAA T T S ++ P I SSVF A
Sbjct: 582 ICCLAFASGRGM*IRLSNRRRTAWSRAYGVFVAARTRTLSSLWPTPCICTRSSVFILLEA 403
Query: 283 SCSL 272
S SL
Sbjct: 402 SLSL 391
>gb|BU978492.1|BU978492 HA13G08r HA Hordeum vulgare subsp. vulgare cDNA clone HA13G08
5-PRIME, mRNA sequence
Length = 544
Score = 192 bits (414), Expect = 3e-048
Identities = 79/80 (98%), Positives = 79/80 (98%)
Frame = +3 / +1
Query: 3 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVK 182
PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATEC TTFFNISASSIVSKWRGDSEKLVK
Sbjct: 304 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECNTTFFNISASSIVSKWRGDSEKLVK 483
Query: 183 VLFELARHHAPSTIFLDEID 242
VLFELARHHAPSTIFLDEID
Sbjct: 484 VLFELARHHAPSTIFLDEID 543
Score = 171 bits (368), Expect = 7e-042
Identities = 72/80 (90%), Positives = 76/80 (95%)
Frame = -1 / -3
Query: 241 SISSRNIVDGA*CLASSNRTLTSFSESPRHLLTIDDAEMLKKVVLHSVATAFASIVFPVP 62
SISSRNIVDGA*CLASS RTLTSFSESPRHLLTID+AEMLK VVLHSVATA A++VFPVP
Sbjct: 542 SISSRNIVDGA*CLASSKRTLTSFSESPRHLLTIDEAEMLKNVVLHSVATALANMVFPVP 363
Query: 61 GGPKSKMPFHGERRPVKYFG 2
GGPKSKMPFHG+ RPVKYFG
Sbjct: 362 GGPKSKMPFHGDSRPVKYFG 303
Score = 126 bits (270), Expect = 2e-028
Identities = 58/80 (72%), Positives = 60/80 (75%)
Frame = -3 / -2
Query: 242 IYFIKEYCGWCMMPSKLKQNFDKLL*ISTPFADN**C*NVEEGGFALSSDRFCQHCLSCS 63
I IK+YCGWCMM KLK+NFD LL ISTPFADN** NVEE ALSSDRF QH L CS
Sbjct: 543 INLIKKYCGWCMMSCKLKKNFD*LLRISTPFADN**SRNVEECSIALSSDRFGQHGLPCS 364
Query: 62 WGAKK*DAFPWRKETSEVFW 3
W KK*DAFP TSEVFW
Sbjct: 363 WRTKK*DAFPR***TSEVFW 304
Score = 121 bits (258), Expect = 1e-026
Identities = 56/75 (74%), Positives = 58/75 (77%)
Frame = +1 / +2
Query: 1 TQNTSLVSFLHGKASYFLAPQEQERQCWQKRSLLSAKPPSSTFQHHQLSANGVEIQRSLS 180
TQNTSLV + GKASYFL QEQ R CW KRSLLSA SSTF+ HQLSANGVEI RS S
Sbjct: 302 TQNTSLVYYHRGKASYFLVLQEQGRPCWPKRSLLSAILHSSTFRLHQLSANGVEILRS*S 481
Query: 181 KFCLSLLGIMHHPQY 225
KF LSL IMHHPQY
Sbjct: 482 KFFLSLQDIMHHPQY 526
Score = 118 bits (253), Expect = 5e-026
Identities = 48/79 (60%), Positives = 56/79 (70%)
Frame = +2 / +3
Query: 2 PKILHWSPFSMERHLTFWPPRNRKDNAGKSGRY*VQNHLLQHFSIINCQQMAWRFREXXX 181
PKILHWS ++ERHLTFW RNR+D+ G+SGRY*VQ ++LQHF INCQQMAWRF E
Sbjct: 303 PKILHWSTITVERHLTFWSSRNREDHVGQSGRY*VQYYILQHFGFINCQQMAWRF*EASQ 482
Query: 182 XXXXXXXXXXTIHNIP**N 238
TIHNI **+
Sbjct: 483 SSF*ACKTSCTIHNIS**D 539
Score = 118 bits (253), Expect = 5e-026
Identities = 58/81 (71%), Positives = 59/81 (72%)
Frame = -2 / -1
Query: 243 HLFHQGILWMVHDA*QAQTEL*QASLNLHAIC*QLMMLKC*RRWFCTQ*RPLLPALSFLF 64
H HQ ILWMVHD QAQ EL* AS NLHAIC*QLM KC*R +CTQ*RPL P S LF
Sbjct: 544 HQSHQEILWMVHDVLQAQKEL*LASQNLHAIC*QLMKPKC*RM*YCTQ*RPLWPTWSSLF 365
Query: 63 LGGQKVRCLSMEKGDQ*SILG 1
L QKVRCLS DQ*SILG
Sbjct: 364 LEDQKVRCLSTVIVDQ*SILG 302
>gb|BG416606.2|BG416606 HVSMEk0013I06f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0013I06f, mRNA sequence
Length = 679
Score = 115 bits (245), Expect(3) = 9e-048
Identities = 47/83 (56%), Positives = 60/83 (72%)
Frame = +3 / +2
Query: 21 LLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELA 200
LL P KG+LLFGPPGT KT+LAKA+ATE F +I+ S++ SKW GD+EKL K LF A
Sbjct: 17 LLRPCKGVLLFGPPGTRKTLLAKALATEAGANFISITGSTLTSKWFGDAEKLTKALFSFA 196
Query: 201 RHHAPSTIFLDEIDAIISQRGEA 269
AP IF+DE+D+++ RG A
Sbjct: 197 SRLAPVIIFVDEVDSLLGARGGA 265
Score = 86.8 bits (183), Expect(3) = 9e-048
Identities = 36/91 (39%), Positives = 58/91 (63%)
Frame = +3 / +2
Query: 333 TKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIPY 512
+K N + +L ATN P++LD A++RRL +RI V LP+ E R + + LL + + +
Sbjct: 329 SKENQRILILGATNRPFDLDDAVIRRLPRRIYVGLPDAENRNKILKILLAKENIESDFKF 508
Query: 513 DILVEKTEGYSGSDIRLVCKEAAMQPLRRLM 605
D L TEGYSGSD++ +C +A +P++ L+
Sbjct: 509 DELANATEGYSGSDLKNLCIASAYRPVQELL 601
Score = 27.6 bits (54), Expect(3) = 9e-048
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +3 / +2
Query: 276 EHEASRRLKTELLIQMDGL 332
EHEA+R+++ E + DGL
Sbjct: 269 EHEATRKMRNEFMAAWDGL 325
>gb|CA012675.1|CA012675 HT06B18r HT Hordeum vulgare subsp. vulgare cDNA clone HT06B18
5-PRIME, mRNA sequence
Length = 700
Score = 118 bits (252), Expect(2) = 2e-045
Identities = 47/82 (57%), Positives = 62/82 (75%)
Frame = +3 / +2
Query: 24 LSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELAR 203
L P +GILLFGPPGTGKTMLAKA+A E + +F N+S S+I SKW G+ EK V+ LF LA
Sbjct: 11 LKPCRGILLFGPPGTGKTMLAKAIANEAQASFINVSMSTITSKWFGEDEKNVRALFTLAA 190
Query: 204 HHAPSTIFLDEIDAIISQRGEA 269
+P+ IF+DE+D+++ QR A
Sbjct: 191 KVSPTIIFVDEVDSMLGQRNRA 256
Score = 84.9 bits (179), Expect(2) = 2e-045
Identities = 38/92 (41%), Positives = 58/92 (63%)
Frame = +3 / +2
Query: 330 LTKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIP 509
L++ + + VLAATN P++LD A++RR E+RI+V LP + R+ + LL +
Sbjct: 317 LSRPDQKILVLAATNRPFDLDEAIIRRFERRIMVGLPSVQNREMIMRRLLSKEKVDEGLD 496
Query: 510 YDILVEKTEGYSGSDIRLVCKEAAMQPLRRLM 605
Y L TEGYSGSD++ +C AA +P+R L+
Sbjct: 497 YKELGTITEGYSGSDLKNLCTTAAYRPVRELI 592
>gb|AV916050.1|AV916050 AV916050 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags1e02 5', mRNA sequence
Length = 552
Score = 94.5 bits (200), Expect(4) = 7e-044
Identities = 42/76 (55%), Positives = 54/76 (71%)
Frame = +3 / +2
Query: 273 SEHEASRRLKTELLIQMDGLTKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEA 452
+E EASRR+KTELL+QM G+ +D V VLAATN P+ LD A+ RR +KRI +PLP+ +A
Sbjct: 197 NESEASRRIKTELLVQMQGVGHNDDKVLVLAATNTPYALDQAVRRRFDKRIYIPLPDAKA 376
Query: 453 RQAMFEELLPATTSKL 500
RQ MF+ L T L
Sbjct: 377 RQHMFKVHLGDTPHSL 424
Score = 52.4 bits (108), Expect(4) = 7e-044
Identities = 21/30 (70%), Positives = 26/30 (86%)
Frame = +3 / +1
Query: 81 LAKAVATECKTTFFNISASSIVSKWRGDSE 170
LAKAVATE +TFF+IS+S +VSKW G+SE
Sbjct: 7 LAKAVATEADSTFFSISSSDLVSKWMGESE 96
Score = 49.2 bits (101), Expect(4) = 7e-044
Identities = 20/43 (46%), Positives = 28/43 (65%)
Frame = +3 / +2
Query: 165 SEKLVKVLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASR 293
+ KLV LF++AR +APS IF+DEID++ RGE + R
Sbjct: 92 AXKLVANLFQMARENAPSIIFIDEIDSLCGTRGEGNESEASRR 220
Score = 38.2 bits (77), Expect(4) = 7e-044
Identities = 12/38 (31%), Positives = 24/38 (63%)
Frame = +3 / +2
Query: 501 EIPYDILVEKTEGYSGSDIRLVCKEAAMQPLRRLMSVL 614
E +++L +TEG+SGSD+ + K+ +P+R+ +
Sbjct: 428 ESDFEVLGRRTEGFSGSDVAVCVKDVLFEPVRKTQDAM 541
>gb|CA006036.1|CA006036 HU05K13u HU Hordeum vulgare subsp. vulgare cDNA clone HU05K13
3-PRIME, mRNA sequence
Length = 483
Score = 171 bits (368), Expect = 7e-042
Identities = 72/99 (72%), Positives = 77/99 (77%)
Frame = +3 / -2
Query: 477 LPATTSKLEIPYDILVEKTEGYSGSDIRLVCKEAAMQPLRRLMSVLEASDXXXXXXXXXX 656
LP T +EIPYD LVE TEGYSGSDIRLVCKEAAMQPLRRLM+VLE
Sbjct: 482 LPLTRDTMEIPYDALVENTEGYSGSDIRLVCKEAAMQPLRRLMAVLEGRQEEIPEDELPE 303
Query: 657 XGPLKPDDIELALRNTRPSAHLHAHRYEKFNQDYGSHVV 773
GP+ +DIELALRNTRPSAHLHAHRYEKFNQDYGSHV+
Sbjct: 302 VGPIATEDIELALRNTRPSAHLHAHRYEKFNQDYGSHVL 186
Score = 115 bits (246), Expect = 4e-025
Identities = 58/98 (59%), Positives = 60/98 (61%)
Frame = -1 / +1
Query: 769 TWLP*SWLNFS*RCAWRCADGLVFLNASSIXXXXXXXXXXXXXXXXXXXXXSRTDMRRLS 590
TWLP*S LNFS CAWR ADG VFL ASSI SRT + RLS
Sbjct: 190 TWLP*S*LNFSYLCAWR*ADGRVFLRASSISSVAIGPTSGNSSSGISSCLPSRTAINRLS 369
Query: 589 GCMAASLHTRRISEPE*PSVFSTSMSYGISNFEVVAGR 476
GC+AASL TRRISEPE PSVFST SYGIS V GR
Sbjct: 370 GCIAASLQTRRISEPEYPSVFSTRASYGISIVSRVNGR 483
>gb|CA031881.1|CA031881 HX11G09r HX Hordeum vulgare subsp. vulgare cDNA clone HX11G09
5-PRIME, mRNA sequence
Length = 620
Score = 163 bits (351), Expect = 1e-039
Identities = 67/68 (98%), Positives = 67/68 (98%)
Frame = +3 / +3
Query: 3 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVK 182
PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATEC TTFFNISASSIVSKWRGDSEKLVK
Sbjct: 417 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECNTTFFNISASSIVSKWRGDSEKLVK 596
Query: 183 VLFELARH 206
VLFELARH
Sbjct: 597 VLFELARH 620
Score = 147 bits (316), Expect = 1e-034
Identities = 60/68 (88%), Positives = 64/68 (94%)
Frame = -1 / -2
Query: 205 CLASSNRTLTSFSESPRHLLTIDDAEMLKKVVLHSVATAFASIVFPVPGGPKSKMPFHGE 26
CLASS RTLTSFSESPRHLLTID+AEMLK VVLHSVATA A++VFPVPGGPKSKMPFHG+
Sbjct: 619 CLASSKRTLTSFSESPRHLLTIDEAEMLKNVVLHSVATALANMVFPVPGGPKSKMPFHGD 440
Query: 25 RRPVKYFG 2
RPVKYFG
Sbjct: 439 SRPVKYFG 416
Score = 110 bits (235), Expect = 1e-023
Identities = 41/57 (71%), Positives = 48/57 (84%)
Frame = +2 / +2
Query: 2 PKILHWSPFSMERHLTFWPPRNRKDNAGKSGRY*VQNHLLQHFSIINCQQMAWRFRE 172
PKILHWS ++ERHLTFW RNR+D+ G+SGRY*VQ ++LQHF INCQQMAWRF E
Sbjct: 416 PKILHWSTITVERHLTFWSSRNREDHVGQSGRY*VQYYILQHFGFINCQQMAWRF*E 586
Score = 102 bits (217), Expect = 4e-021
Identities = 49/66 (74%), Positives = 51/66 (77%)
Frame = +1 / +1
Query: 1 TQNTSLVSFLHGKASYFLAPQEQERQCWQKRSLLSAKPPSSTFQHHQLSANGVEIQRSLS 180
TQNTSLV + GKASYFL QEQ R CW KRSLLSA SSTF+ HQLSANGVEI RS S
Sbjct: 415 TQNTSLVYYHRGKASYFLVLQEQGRPCWPKRSLLSAILHSSTFRLHQLSANGVEILRS*S 594
Query: 181 KFCLSL 198
KF LSL
Sbjct: 595 KFFLSL 612
>gb|AV915850.1|AV915850 AV915850 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags16e05 5', mRNA sequence
Length = 625
Score = 86.8 bits (183), Expect(3) = 3e-039
Identities = 36/91 (39%), Positives = 58/91 (63%)
Frame = +3 / +1
Query: 333 TKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIPY 512
+K N + +L ATN P++LD A++RRL +RI V LP+ E R + + LL + + +
Sbjct: 268 SKENQRILILGATNRPFDLDDAVIRRLPRRIYVGLPDAENRNKILKILLAKENIESDFKF 447
Query: 513 DILVEKTEGYSGSDIRLVCKEAAMQPLRRLM 605
D L TEGYSGSD++ +C +A +P++ L+
Sbjct: 448 DELANATEGYSGSDLKNLCIASAYRPVQELL 540
Score = 86.3 bits (182), Expect(3) = 3e-039
Identities = 35/67 (52%), Positives = 47/67 (70%)
Frame = +3 / +1
Query: 69 GKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAI 248
GKT+LAKA+ATE F +I+ S++ SKW GD+EKL K LF A AP IF+DE+D++
Sbjct: 4 GKTLLAKALATEAGANFISITGSTLTSKWFGDAEKLTKALFSFASRLAPVIIFVDEVDSL 183
Query: 249 ISQRGEA 269
+ RG A
Sbjct: 184 LGARGGA 204
Score = 27.6 bits (54), Expect(3) = 3e-039
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +3 / +1
Query: 276 EHEASRRLKTELLIQMDGL 332
EHEA+R+++ E + DGL
Sbjct: 208 EHEATRKMRNEFMAAWDGL 264
>gb|BE060343.3|BE060343 HVSMEg0012A18f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0012A18f, mRNA sequence
Length = 710
Score = 116 bits (247), Expect(3) = 5e-037
Identities = 45/80 (56%), Positives = 60/80 (75%)
Frame = +3 / +3
Query: 21 LLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELA 200
LLSP KG+LL+GPPGTGKTMLAKA+A E F N+ S+++SKW GD++KLV +F LA
Sbjct: 54 LLSPQKGVLLYGPPGTGKTMLAKAIAKESGAVFINVRISNLMSKWFGDAQKLVSAVFSLA 233
Query: 201 RHHAPSTIFLDEIDAIISQR 260
P+ IF+DE+D+ + QR
Sbjct: 234 NKLQPAIIFIDEVDSFLGQR 293
Score = 52.4 bits (108), Expect(3) = 5e-037
Identities = 28/83 (33%), Positives = 39/83 (46%)
Frame = +3 / +3
Query: 333 TKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIPY 512
T N V VLAATN P ELD A L R + + +P R +L + I Y
Sbjct: 363 TDQNARVMVLAATNRPXELDEAXLXRFTQIFEIGVPSRSERSKXTXVILXGENVESNIDY 542
Query: 513 DILVEKTEGYSGSDIRLVCKEAA 581
+ + EG++ SD +C+ AA
Sbjct: 543 EYIASLCEGFTWSDXLELCRXAA 611
Score = 24.9 bits (48), Expect(3) = 5e-037
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +3 / +3
Query: 273 SEHEASRRLKTELLIQMDGLT 335
++HEA +KTE + DG T
Sbjct: 300 TDHEALTNMKTEFMSLWDGFT 362
>gb|BJ447407.1|BJ447407 BJ447407 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak35g05 5', mRNA sequence
Length = 635
Score = 118 bits (253), Expect(3) = 7e-037
Identities = 48/83 (57%), Positives = 61/83 (73%)
Frame = +3 / +1
Query: 21 LLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELA 200
LL P KG+LLFGPPGTGKT+LAKA+ATE F +I+ S++ SKW GD+EKL K LF A
Sbjct: 208 LLRPCKGVLLFGPPGTGKTLLAKALATEAGANFISITGSTLTSKWFGDAEKLTKALFSFA 387
Query: 201 RHHAPSTIFLDEIDAIISQRGEA 269
AP IF+DE+D+++ RG A
Sbjct: 388 SRLAPVIIFVDEVDSLLGARGGA 456
Score = 46.4 bits (95), Expect(3) = 7e-037
Identities = 18/37 (48%), Positives = 27/37 (72%)
Frame = +3 / +1
Query: 333 TKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPE 443
+K N + +L ATN P++LD A++RRL +RI V LP+
Sbjct: 520 SKENQRILILGATNRPFDLDDAVIRRLPRRIYVGLPD 630
Score = 27.6 bits (54), Expect(3) = 7e-037
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +3 / +1
Query: 276 EHEASRRLKTELLIQMDGL 332
EHEA+R+++ E + DGL
Sbjct: 460 EHEATRKMRNEFMAAWDGL 516
>gb|BJ463449.1|BJ463449 BJ463449 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags27m14 5', mRNA sequence
Length = 437
Score = 122 bits (261), Expect(3) = 4e-035
Identities = 52/80 (65%), Positives = 60/80 (75%)
Frame = +3 / +2
Query: 21 LLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELA 200
L+ P KGILLFGPPGTGKTMLAKAVATE F NIS SSI SKW G+ EK VK +F LA
Sbjct: 53 LMKPCKGILLFGPPGTGKTMLAKAVATEAGANFINISMSSIGSKWFGEGEKYVKAVFSLA 232
Query: 201 RHHAPSTIFLDEIDAIISQR 260
APS IF+DE+D ++ +R
Sbjct: 233 SKIAPSVIFVDEVDGMLGRR 292
Score = 32.2 bits (64), Expect(3) = 4e-035
Identities = 13/24 (54%), Positives = 18/24 (75%)
Frame = +3 / +2
Query: 333 TKTNDLVFVLAATNLPWELDAAML 404
TK + V VLAATN P++LD A++
Sbjct: 365 TKDKERVLVLAATNRPFDLDEAVI 436
Score = 32.2 bits (64), Expect(3) = 4e-035
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3 / +2
Query: 258 RGEARSEHEASRRLKTELLIQMDGL 332
R E EHEA R++K E ++ DGL
Sbjct: 287 RRENPGEHEAMRKMKNEFMVNWDGL 361
>gb|AV924480.1|AV924480 AV924480 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd14l20 5', mRNA sequence
Length = 604
Score = 92.3 bits (195), Expect(3) = 7e-035
Identities = 40/92 (43%), Positives = 61/92 (66%)
Frame = +3 / +2
Query: 330 LTKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIP 509
L+K+ + + VLAATN P++LD A++RR E+RI+V LP E+R+ + +L +I
Sbjct: 248 LSKSGERILVLAATNRPFDLDEAIIRRFERRIMVGLPTQESRELILRTVLSKEKVDKDIE 427
Query: 510 YDILVEKTEGYSGSDIRLVCKEAAMQPLRRLM 605
Y L TEGYSGSD++ +C AA +P+R L+
Sbjct: 428 YKELATMTEGYSGSDLKNLCVTAAYRPVR*LL 523
Score = 66.6 bits (139), Expect(3) = 7e-035
Identities = 26/55 (47%), Positives = 38/55 (69%)
Frame = +3 / +2
Query: 96 ATECKTTFFNISASSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQR 260
A + +F N+S S+I SKW G+ EK V+ LF LA AP+ IF+DE+D+++ QR
Sbjct: 14 ANDAGASFINVSMSTITSKWFGEDEKNVRALFSLAAKVAPTIIFVDEVDSMLGQR 178
Score = 27.2 bits (53), Expect(3) = 7e-035
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +3 / +2
Query: 276 EHEASRRLKTELLIQMDGL 332
EHEA R++K E + DG+
Sbjct: 191 EHEAMRKIKNEFMSHWDGI 247
>gb|AV924479.1|AV924479 AV924479 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd14l19 5', mRNA sequence
Length = 584
Score = 92.3 bits (195), Expect(3) = 7e-035
Identities = 40/92 (43%), Positives = 61/92 (66%)
Frame = +3 / +3
Query: 330 LTKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIP 509
L+K+ + + VLAATN P++LD A++RR E+RI+V LP E+R+ + +L +I
Sbjct: 237 LSKSGERILVLAATNRPFDLDEAIIRRFERRIMVGLPTQESRELILRTVLSKEKVDKDIE 416
Query: 510 YDILVEKTEGYSGSDIRLVCKEAAMQPLRRLM 605
Y L TEGYSGSD++ +C AA +P+R L+
Sbjct: 417 YKELATMTEGYSGSDLKNLCVTAAYRPVR*LL 512
Score = 66.6 bits (139), Expect(3) = 7e-035
Identities = 26/55 (47%), Positives = 38/55 (69%)
Frame = +3 / +3
Query: 96 ATECKTTFFNISASSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQR 260
A + +F N+S S+I SKW G+ EK V+ LF LA AP+ IF+DE+D+++ QR
Sbjct: 3 ANDAGASFINVSMSTITSKWFGEDEKNVRALFSLAAKVAPTIIFVDEVDSMLGQR 167
Score = 27.2 bits (53), Expect(3) = 7e-035
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +3 / +3
Query: 276 EHEASRRLKTELLIQMDGL 332
EHEA R++K E + DG+
Sbjct: 180 EHEAMRKIKNEFMSHWDGI 236
>gb|BE437488.1|BE437488 SFR004.C06F990621 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 535
Score = 138 bits (296), Expect(2) = 1e-034
Identities = 58/78 (74%), Positives = 62/78 (79%)
Frame = +3 / +1
Query: 540 YSGSDIRLVCKEAAMQPLRRLMSVLEASDXXXXXXXXXXXGPLKPDDIELALRNTRPSAH 719
YSGSDIRLVCKEAAMQPLRRLM+VLE GP+ +DIELALRNTRPSAH
Sbjct: 58 YSGSDIRLVCKEAAMQPLRRLMAVLEGRQEEIPEDELPEVGPIATEDIELALRNTRPSAH 237
Query: 720 LHAHRYEKFNQDYGSHVV 773
LHAHRYEKFNQDYGSHV+
Sbjct: 238 LHAHRYEKFNQDYGSHVL 291
Score = 28.6 bits (56), Expect(2) = 1e-034
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = +3 / +2
Query: 486 TTSKLEIPYDILVEKTE 536
T +EIPYD LVE TE
Sbjct: 5 TRDTMEIPYDALVENTE 55
>gb|BE195361.2|BE195361 HVSMEh0089A10f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0089A10f, mRNA sequence
Length = 982
Score = 94.5 bits (200), Expect(3) = 1e-033
Identities = 42/76 (55%), Positives = 54/76 (71%)
Frame = +3 / +1
Query: 273 SEHEASRRLKTELLIQMDGLTKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEA 452
+E EASRR+KTELL+QM G+ +D V VLAATN P+ LD A+ RR +KRI +PLP+ +A
Sbjct: 100 NESEASRRIKTELLVQMQGVGHNDDKVLVLAATNTPYALDQAVRRRFDKRIYIPLPDAKA 279
Query: 453 RQAMFEELLPATTSKL 500
RQ MF+ L T L
Sbjct: 280 RQHMFKVHLGDTPHSL 327
Score = 49.2 bits (101), Expect(3) = 1e-033
Identities = 20/41 (48%), Positives = 27/41 (65%)
Frame = +3 / +1
Query: 171 KLVKVLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASR 293
KLV LF++AR +APS IF+DEID++ RGE + R
Sbjct: 1 KLVANLFQMARENAPSIIFIDEIDSLCGTRGEGNESEASRR 123
Score = 38.2 bits (77), Expect(3) = 1e-033
Identities = 12/38 (31%), Positives = 24/38 (63%)
Frame = +3 / +1
Query: 501 EIPYDILVEKTEGYSGSDIRLVCKEAAMQPLRRLMSVL 614
E +++L +TEG+SGSD+ + K+ +P+R+ +
Sbjct: 331 ESDFEVLGRRTEGFSGSDVAVCVKDVLFEPVRKTQDAM 444
>gb|BF616546.2|BF616546 HVSMEc0010G23f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0010G23f, mRNA sequence
Length = 745
Score = 116 bits (247), Expect(3) = 1e-033
Identities = 45/80 (56%), Positives = 60/80 (75%)
Frame = +3 / +2
Query: 21 LLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELA 200
LLSP KG+LL+GPPGTGKTMLAKA+A E F N+ S+++SKW GD++KLV +F LA
Sbjct: 245 LLSPQKGVLLYGPPGTGKTMLAKAIAKESGAVFINVRISNLMSKWFGDAQKLVSAVFSLA 424
Query: 201 RHHAPSTIFLDEIDAIISQR 260
P+ IF+DE+D+ + QR
Sbjct: 425 NKLQPAIIFIDEVDSFLGQR 484
Score = 40.9 bits (83), Expect(3) = 1e-033
Identities = 22/63 (34%), Positives = 31/63 (49%)
Frame = +3 / +2
Query: 333 TKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIPY 512
T N V VLAATN P ELD A+LRR + + +P + + +L + I Y
Sbjct: 554 TDQNARVMVLAATNRPSELDEAILRRFTQIFEIGVPSRREGSKILQVILEGKDVESNIGY 733
Query: 513 DIL 521
+ L
Sbjct: 734 EYL 742
Score = 24.9 bits (48), Expect(3) = 1e-033
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +3 / +2
Query: 273 SEHEASRRLKTELLIQMDGLT 335
++HEA +KTE + DG T
Sbjct: 491 TDHEALTNMKTEFMSLWDGFT 553
>gb|CA026989.1|CA026989 HZ57I24r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ57I24
5-PRIME, mRNA sequence
Length = 495
Score = 140 bits (301), Expect = 1e-032
Identities = 55/88 (62%), Positives = 71/88 (80%)
Frame = +3 / +2
Query: 3 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVK 182
P++FTG PW+ LL+GPPGTGK+ LAKAVATE +TFF+IS+S +VSKW G+SEKLV
Sbjct: 215 PQFFTGKRRPWRAFLLYGPPGTGKSYLAKAVATEADSTFFSISSSDLVSKWMGESEKLVA 394
Query: 183 VLFELARHHAPSTIFLDEIDAIISQRGE 266
LF++AR +APS IF+DEID++ RGE
Sbjct: 395 NLFQMARENAPSIIFIDEIDSLCGTRGE 478
>gb|BU989522.1|BU989522 HF21P14r HF Hordeum vulgare subsp. vulgare cDNA clone HF21P14
5-PRIME, mRNA sequence
Length = 659
Score = 79.9 bits (168), Expect(3) = 9e-032
Identities = 33/91 (36%), Positives = 55/91 (60%)
Frame = +3 / +3
Query: 333 TKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIPY 512
TK + V VL ATN P++LD A++RR +R++V LP+ R+ + + +L +
Sbjct: 225 TKDKERVLVLGATNRPFDLDEAVIRRFPRRLMVNLPDASNREKILKVILAKEELGQDTDL 404
Query: 513 DILVEKTEGYSGSDIRLVCKEAAMQPLRRLM 605
+ L T+GYSGSD++ +C AA P+R ++
Sbjct: 405 ESLANMTDGYSGSDLKNLCVTAAHYPIREIL 497
Score = 63.4 bits (132), Expect(3) = 9e-032
Identities = 25/46 (54%), Positives = 34/46 (73%)
Frame = +3 / +3
Query: 123 NISASSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQR 260
NIS SSI SKW G+ EK VK +F LA +PS IF+DE+D+++ +R
Sbjct: 15 NISMSSITSKWFGEGEKYVKAVFSLASKISPSVIFIDEVDSMLGRR 152
Score = 32.2 bits (64), Expect(3) = 9e-032
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3 / +3
Query: 258 RGEARSEHEASRRLKTELLIQMDGL 332
R E EHEA R++K E ++ DGL
Sbjct: 147 RRENPGEHEAMRKMKNEFMVNWDGL 221
>gb|AV913885.1|AV913885 AV913885 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags23k03 5', mRNA sequence
Length = 640
Score = 86.8 bits (183), Expect(3) = 4e-031
Identities = 36/91 (39%), Positives = 58/91 (63%)
Frame = +3 / +2
Query: 333 TKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIPY 512
+K N + +L ATN P++LD A++RRL +RI V LP+ E R + + LL + + +
Sbjct: 215 SKENQRILILGATNRPFDLDDAVIRRLPRRIYVGLPDAENRNKILKILLAKENIESDFKF 394
Query: 513 DILVEKTEGYSGSDIRLVCKEAAMQPLRRLM 605
D L TEGYSGSD++ +C +A +P++ L+
Sbjct: 395 DELANATEGYSGSDLKNLCIASAYRPVQELL 487
Score = 58.8 bits (122), Expect(3) = 4e-031
Identities = 23/45 (51%), Positives = 31/45 (68%)
Frame = +3 / +2
Query: 135 SSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQRGEA 269
S++ SKW GD+EKL K LF A AP IF+DE+D+++ RG A
Sbjct: 17 STLTSKWFGDAEKLTKALFSFASRLAPVIIFVDEVDSLLGARGGA 151
Score = 27.6 bits (54), Expect(3) = 4e-031
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +3 / +2
Query: 276 EHEASRRLKTELLIQMDGL 332
EHEA+R+++ E + DGL
Sbjct: 155 EHEATRKMRNEFMAAWDGL 211
>gb|BG369694.1|BG369694 HVSMEi0025I17f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0025I17f, mRNA sequence
Length = 692
Score = 122 bits (260), Expect(2) = 9e-031
Identities = 51/80 (63%), Positives = 60/80 (75%)
Frame = +3 / +1
Query: 21 LLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELA 200
L P KGILLFGPPGTGKTMLAKAVATE F NIS SSI SKW G+ EK VK +F LA
Sbjct: 301 LTKPCKGILLFGPPGTGKTMLAKAVATEAGANFINISMSSITSKWFGEGEKYVKAVFSLA 480
Query: 201 RHHAPSTIFLDEIDAIISQR 260
+PS IF+DE+D+++ +R
Sbjct: 481 SKISPSVIFIDEVDSMLGRR 540
Score = 32.2 bits (64), Expect(2) = 9e-031
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3 / +1
Query: 258 RGEARSEHEASRRLKTELLIQMDGL 332
R E EHEA R++K E ++ DGL
Sbjct: 535 RRENPGEHEAMRKMKNEFMVNWDGL 609
>gb|BG344809.1|BG344809 HVSMEg0016C19f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0016C19f, mRNA sequence
Length = 661
Score = 90.9 bits (192), Expect(3) = 1e-030
Identities = 41/76 (53%), Positives = 53/76 (69%)
Frame = +3 / +2
Query: 273 SEHEASRRLKTELLIQMDGLTKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEA 452
+E EASRR+KTELL+QM G+ +D VLAATN P+ LD A+ RR +KRI +PLP+ +A
Sbjct: 200 NESEASRRIKTELLVQMQGVGHNDDKDLVLAATNTPYALDQAVPRRFDKRIYIPLPDAKA 379
Query: 453 RQAMFEELLPATTSKL 500
RQ MF+ L T L
Sbjct: 380 RQHMFKVHLGDTPHSL 427
Score = 53.8 bits (111), Expect(3) = 1e-030
Identities = 23/51 (45%), Positives = 32/51 (62%)
Frame = +3 / +2
Query: 141 IVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASR 293
I + G+S+KLV LF++AR APS IF+DEID++ RGE + R
Sbjct: 71 IXHEGMGESKKLVANLFQIAREGAPSIIFIDEIDSLCGTRGEGNESEASRR 223
Score = 27.2 bits (53), Expect(3) = 1e-030
Identities = 9/38 (23%), Positives = 22/38 (57%)
Frame = +3 / +2
Query: 501 EIPYDILVEKTEGYSGSDIRLVCKEAAMQPLRRLMSVL 614
E + +L +T+G+S S++ + K+ +P+R+ +
Sbjct: 431 ESDF*VLSRRTKGFSCSNVAVCVKDVLFEPVRQTQDAM 544
>gb|BQ472612.1|BQ472612 HB01P21r BC Hordeum vulgare subsp. vulgare cDNA clone HB01P21
5-PRIME, mRNA sequence
Length = 618
Score = 91.3 bits (193), Expect(2) = 1e-030
Identities = 40/92 (43%), Positives = 62/92 (67%)
Frame = +3 / +2
Query: 330 LTKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIP 509
L+ +N+ + VLAATN P++LD A++RR E RI+V LP E+R+ + ++LL + I
Sbjct: 212 LSNSNERILVLAATNRPFDLDEAIVRRFEHRIMVGLPTLESRELILKKLLSKEKVEEGID 391
Query: 510 YDILVEKTEGYSGSDIRLVCKEAAMQPLRRLM 605
+ L TEGYSGSD++ +C AA +P+R L+
Sbjct: 392 FKELATSTEGYSGSDLKNLCVTAAYRPVRELI 487
Score = 62.5 bits (130), Expect(2) = 1e-030
Identities = 24/48 (50%), Positives = 37/48 (77%)
Frame = +3 / +2
Query: 123 NISASSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQRGE 266
NIS S+I+SK+ GD+EK ++ LF LA AP+ IF+DE+D+++ QR +
Sbjct: 5 NISLSTIMSKYYGDAEKTIRALFSLATKLAPAIIFVDEVDSLLGQRDQ 148
>gb|BQ472874.1|BQ472874 HB01D18r BC Hordeum vulgare subsp. vulgare cDNA clone HB01D18
5-PRIME, mRNA sequence
Length = 542
Score = 91.3 bits (193), Expect(2) = 1e-030
Identities = 40/92 (43%), Positives = 62/92 (67%)
Frame = +3 / +2
Query: 330 LTKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIP 509
L+ +N+ + VLAATN P++LD A++RR E RI+V LP E+R+ + ++LL + I
Sbjct: 212 LSNSNERILVLAATNRPFDLDEAIVRRFEHRIMVGLPTLESRELILKKLLSKEKVEEGID 391
Query: 510 YDILVEKTEGYSGSDIRLVCKEAAMQPLRRLM 605
+ L TEGYSGSD++ +C AA +P+R L+
Sbjct: 392 FKELATSTEGYSGSDLKNLCVTAAYRPVRELI 487
Score = 62.5 bits (130), Expect(2) = 1e-030
Identities = 24/48 (50%), Positives = 37/48 (77%)
Frame = +3 / +2
Query: 123 NISASSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQRGE 266
NIS S+I+SK+ GD+EK ++ LF LA AP+ IF+DE+D+++ QR +
Sbjct: 5 NISLSTIMSKYYGDAEKTIRALFSLATKLAPAIIFVDEVDSLLGQRDQ 148
>gb|CK124523.1|CK124523 BES1824109h02 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010H029 5-PRIME, mRNA sequence
Length = 690
Score = 105 bits (225), Expect(4) = 2e-030
Identities = 39/85 (45%), Positives = 59/85 (69%)
Frame = +3 / +1
Query: 24 LSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELAR 203
+SP KG+L +GPPG GKT+LAKA+A EC+ F +I +++ W G+SE V+ +F+ AR
Sbjct: 55 MSPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKAR 234
Query: 204 HHAPSTIFLDEIDAIISQRGEARSE 278
AP +F DE+D+I +QRG + +
Sbjct: 235 GSAPCVLFFDELDSIATQRGSSSGD 309
Score = 30.9 bits (61), Expect(4) = 2e-030
Identities = 13/44 (29%), Positives = 25/44 (56%)
Frame = +3 / +1
Query: 408 RLEKRILVPLPEGEARQAMFEELLPATTSKLEIPYDILVEKTEG 539
RL++ I +PLP+ ++R +F+ L + +I L + T+G
Sbjct: 445 RLDQLIYIPLPDVDSRHQIFKACLRKSPLAKDIDLSALAKYTQG 576
Score = 25.8 bits (50), Expect(4) = 2e-030
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = +3 / +2
Query: 516 ILVEKTEGYSGSDIRLVCKEAAMQPLR 596
+L +G+SG+DI +C+ A +R
Sbjct: 554 LLPSTPKGFSGADITEICQRACKYAIR 634
Score = 25.8 bits (50), Expect(4) = 2e-030
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +3 / +1
Query: 351 VFVLAATNLPWELDAAMLR 407
VF++ ATN P +D A+LR
Sbjct: 382 VFIIGATNRPDIIDPALLR 438
>gb|BM098487.2|BM098487 EBem08_SQ003_D06_R embryo, 40 DPA, no treatment, cv Optic, EBem08
Hordeum vulgare subsp. vulgare cDNA clone
EBem08_SQ003_D06 5', mRNA sequence
Length = 524
Score = 122 bits (262), Expect(2) = 2e-030
Identities = 49/84 (58%), Positives = 64/84 (76%)
Frame = +3 / +1
Query: 18 GLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFEL 197
GLL P +GILLFGPPGTGKTMLAKA+A E + +F N+S S+I SKW G+ EK V+ LF L
Sbjct: 148 GLLKPCRGILLFGPPGTGKTMLAKAIANEAQASFINVSMSTITSKWFGEDEKNVRALFTL 327
Query: 198 ARHHAPSTIFLDEIDAIISQRGEA 269
A +P+ IF+DE+D+++ QR A
Sbjct: 328 AAKVSPTIIFVDEVDSMLGQRNRA 399
Score = 29.9 bits (59), Expect(2) = 2e-030
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +3 / +1
Query: 276 EHEASRRLKTELLIQMDGLTKTNDLVFVLAA 368
EHEA R++K E + DGL D ++ A
Sbjct: 403 EHEAMRKIKNEFMTHWDGLLSRPDQKILVLA 495
>gb|BI950087.1|BI950087 HVSMEl0018H22f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0018H22f, mRNA sequence
Length = 884
Score = 105 bits (225), Expect(3) = 3e-030
Identities = 39/85 (45%), Positives = 59/85 (69%)
Frame = +3 / +1
Query: 24 LSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELAR 203
+SP KG+L +GPPG GKT+LAKA+A EC+ F +I +++ W G+SE V+ +F+ AR
Sbjct: 205 MSPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKAR 384
Query: 204 HHAPSTIFLDEIDAIISQRGEARSE 278
AP +F DE+D+I +QRG + +
Sbjct: 385 GSAPCVLFFDELDSIATQRGSSSGD 459
Score = 38.6 bits (78), Expect(3) = 3e-030
Identities = 17/57 (29%), Positives = 32/57 (56%)
Frame = +3 / +1
Query: 408 RLEKRILVPLPEGEARQAMFEELLPATTSKLEIPYDILVEKTEGYSGSDIRLVCKEA 578
RL++ I +PLP+ ++R +F L + +I L + T+ ++G+DI C+ A
Sbjct: 595 RLDQLIYIPLPDVDSRHQIFXACLRKSPLAKDIDLSALAKYTQRFNGADIT*NCQRA 765
Score = 25.8 bits (50), Expect(3) = 3e-030
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +3 / +1
Query: 351 VFVLAATNLPWELDAAMLR 407
VF++ ATN P +D A+LR
Sbjct: 532 VFIIGATNRPDIIDPALLR 588
>gb|BJ450504.1|BJ450504 BJ450504 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak29p10 5', mRNA sequence
Length = 627
Score = 59.3 bits (123), Expect(5) = 7e-030
Identities = 27/53 (50%), Positives = 34/53 (64%)
Frame = +3 / +1
Query: 342 NDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKL 500
+DLV V+ ATN P ELD A+LRRL KRI VPLP+ R+ + + L KL
Sbjct: 235 DDLVIVIGATNKPQELDDAVLRRLVKRIYVPLPDPNVRRLLLKNQLKGQAFKL 393
Score = 48.3 bits (99), Expect(5) = 7e-030
Identities = 18/28 (64%), Positives = 23/28 (82%)
Frame = +3 / +1
Query: 519 LVEKTEGYSGSDIRLVCKEAAMQPLRRL 602
L +TEGYSGSD+R +C+EAAM P+R L
Sbjct: 415 LAVETEGYSGSDLRALCEEAAMMPIREL 498
Score = 37.3 bits (75), Expect(5) = 7e-030
Identities = 15/24 (62%), Positives = 19/24 (79%)
Frame = +3 / +1
Query: 276 EHEASRRLKTELLIQMDGLTKTND 347
E++ASRRLK+E LIQ DG+T D
Sbjct: 166 ENDASRRLKSEFLIQFDGVTXNPD 237
Score = 31.8 bits (63), Expect(5) = 7e-030
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +3 / +1
Query: 189 FELARHHAPSTIFLDEIDAIISQR 260
F +A PS IF+DEID+++S R
Sbjct: 85 FXVAVERQPSVIFMDEIDSVMSTR 156
Score = 26.7 bits (52), Expect(5) = 7e-030
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = +3 / +2
Query: 105 CKTTFFNISASSIVSKWRGDSEKLVKVL 188
C+ F + + + KW G++EKLV+ L
Sbjct: 2 CREAHFLMFSFFVDIKWVGEAEKLVRTL 85
>gb|BF260432.3|BF260432 HVSMEf0021P16f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0021P16f, mRNA sequence
Length = 832
Score = 109 bits (233), Expect(3) = 1e-029
Identities = 45/86 (52%), Positives = 59/86 (68%)
Frame = +3 / +2
Query: 21 LLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELA 200
LLSP KG+LL+GPPGTGKTMLAKA+A E F N+ S+++SKW GD++KLV +F LA
Sbjct: 188 LLSPQKGVLLYGPPGTGKTMLAKAIAKESGARFINVRISNLMSKWFGDAQKLVSAVFSLA 367
Query: 201 RHHAPSTIFLDEIDAIISQRGEARSE 278
P+ IF+DE + + QR E
Sbjct: 368 NKLQPAIIFIDEGRSFLGQRRNTNHE 445
Score = 35.0 bits (70), Expect(3) = 1e-029
Identities = 17/26 (65%), Positives = 17/26 (65%)
Frame = +3 / +2
Query: 333 TKTNDLVFVLAATNLPWELDAAMLRR 410
T N V VLAATN P ELD A LRR
Sbjct: 497 TDQNARVMVLAATNRPSELDEAFLRR 574
Score = 24.0 bits (46), Expect(3) = 1e-029
Identities = 9/21 (42%), Positives = 12/21 (57%)
Frame = +3 / +2
Query: 273 SEHEASRRLKTELLIQMDGLT 335
+ HEA +KTE + DG T
Sbjct: 434 TNHEALTNMKTEFMSLWDGFT 496
>gb|BQ459038.1|BQ459038 HA02H18r HA Hordeum vulgare subsp. vulgare cDNA clone HA02H18
5-PRIME, mRNA sequence
Length = 634
Score = 100 bits (213), Expect(3) = 5e-029
Identities = 41/87 (47%), Positives = 59/87 (67%)
Frame = +3 / +1
Query: 24 LSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELAR 203
+ P KG++L+G PGTGKT+LAKAVA TF + S ++ K+ GD KLV+ LF +A
Sbjct: 34 IRPPKGVILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVAD 213
Query: 204 HHAPSTIFLDEIDAIISQRGEARSEHE 284
+PS +F+DEIDA+ ++R +A S E
Sbjct: 214 ELSPSIVFIDEIDAVGTKRYDAHSGGE 294
Score = 41.4 bits (84), Expect(3) = 5e-029
Identities = 17/63 (26%), Positives = 36/63 (57%)
Frame = +3 / +1
Query: 408 RLEKRILVPLPEGEARQAMFEELLPATTSKLEIPYDILVEKTEGYSGSDIRLVCKEAAMQ 587
R++++I PLP+ + R+ +F+ T ++ + V + +SG+DI+ +C E+ +
Sbjct: 424 RIDRKIEFPLPDIKTRRRIFQIHTAKMTLSDDVNLEEFVMTKDEFSGADIKAICTESGLL 603
Query: 588 PLR 596
LR
Sbjct: 604 ALR 612
Score = 24.4 bits (47), Expect(3) = 5e-029
Identities = 10/29 (34%), Positives = 15/29 (51%)
Frame = +3 / +1
Query: 291 RRLKTELLIQMDGLTKTNDLVFVLAATNL 377
+R ELL Q+DG D+ +LA +
Sbjct: 304 QRTMLELLNQLDGFDSRGDVKVILATNRI 390
>gb|BQ469784.1|BQ469784 HZ01N19r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ01N19
5-PRIME, mRNA sequence
Length = 477
Score = 127 bits (272), Expect = 1e-028
Identities = 51/82 (62%), Positives = 67/82 (81%)
Frame = +3 / +1
Query: 3 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVK 182
P++FTG PW+ LL+GPPGTGK+ LAKAVATE +TFF+IS+S +VSK G+SEKLV
Sbjct: 229 PQFFTGKRRPWRAFLLYGPPGTGKSYLAKAVATEADSTFFSISSSDLVSK*MGESEKLVA 408
Query: 183 VLFELARHHAPSTIFLDEIDAI 248
LF++AR +APS IF+DEID++
Sbjct: 409 NLFQMARENAPSIIFIDEIDSL 474
>gb|BE421366.1|BE421366 HWM008.E11 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM008.E11, mRNA sequence
Length = 1011
Score = 78.0 bits (164), Expect(3) = 2e-028
Identities = 35/92 (38%), Positives = 52/92 (56%)
Frame = +3 / +1
Query: 333 TKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIPY 512
T N V VLAATN P ELD A+LRR + + +P R + + +L + I Y
Sbjct: 355 TDQNARVMVLAATNRPSELDEAILRRFTQIFEIGVPSRSERSKILQVILKGENVESNIDY 534
Query: 513 DILVEKTEGYSGSDIRLVCKEAAMQPLRRLMS 608
D + EG++GSDI +CK+AA P+R +++
Sbjct: 535 DYIASLCEGFTGSDILELCKQAAFYPIREILN 630
Score = 61.6 bits (128), Expect(3) = 2e-028
Identities = 22/48 (45%), Positives = 34/48 (70%)
Frame = +3 / +1
Query: 117 FFNISASSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAIISQR 260
F N+ S+++SKW GD++KLV +F LA P+ IF+DE+D+ + QR
Sbjct: 142 FMNVRISNLMSKWFGDAQKLVSAVFSLANKLQPAIIFIDEVDSFLGQR 285
Score = 24.9 bits (48), Expect(3) = 2e-028
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +3 / +1
Query: 273 SEHEASRRLKTELLIQMDGLT 335
++HEA +KTE + DG T
Sbjct: 292 TDHEALTNMKTEFMSLWDGFT 354
>gb|AV916179.1|AV916179 AV916179 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags1p16 5', mRNA sequence
Length = 578
Score = 105 bits (225), Expect(3) = 5e-028
Identities = 39/85 (45%), Positives = 59/85 (69%)
Frame = +3 / +3
Query: 24 LSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELAR 203
+SP KG+L +GPPG GKT+LAKA+A EC+ F +I +++ W G+SE V+ +F+ AR
Sbjct: 6 MSPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKAR 185
Query: 204 HHAPSTIFLDEIDAIISQRGEARSE 278
AP +F DE+D+I +QRG + +
Sbjct: 186 GSAPCVLFFDELDSIATQRGSSSGD 260
Score = 30.9 bits (61), Expect(3) = 5e-028
Identities = 13/44 (29%), Positives = 25/44 (56%)
Frame = +3 / +3
Query: 408 RLEKRILVPLPEGEARQAMFEELLPATTSKLEIPYDILVEKTEG 539
RL++ I +PLP+ ++R +F+ L + +I L + T+G
Sbjct: 396 RLDQLIYIPLPDVDSRHQIFKACLRKSPLAKDIDLSALAKYTQG 527
Score = 25.8 bits (50), Expect(3) = 5e-028
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +3 / +3
Query: 351 VFVLAATNLPWELDAAMLR 407
VF++ ATN P +D A+LR
Sbjct: 333 VFIIGATNRPDIIDPALLR 389
>gb|BU979682.1|BU979682 HA16O21r HA Hordeum vulgare subsp. vulgare cDNA clone HA16O21
5-PRIME, mRNA sequence
Length = 616
Score = 122 bits (260), Expect = 5e-027
Identities = 51/80 (63%), Positives = 60/80 (75%)
Frame = +3 / +2
Query: 21 LLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELA 200
L P KGILLFGPPGTGKTMLAKAVATE F NIS SSI SKW G+ EK VK +F LA
Sbjct: 320 LTKPCKGILLFGPPGTGKTMLAKAVATEAGANFINISMSSITSKWFGEGEKYVKAVFSLA 499
Query: 201 RHHAPSTIFLDEIDAIISQR 260
+PS IF+DE+D+++ +R
Sbjct: 500 SKISPSVIFIDEVDSMLGRR 559
>gb|CA011901.1|CA011901 HT03N20r HT Hordeum vulgare subsp. vulgare cDNA clone HT03N20
5-PRIME, mRNA sequence
Length = 399
Score = 120 bits (257), Expect = 1e-026
Identities = 51/91 (56%), Positives = 61/91 (67%)
Frame = +3 / +3
Query: 21 LLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELA 200
LL P KGILLFGPPGTGKT+LAKA+ATE F +I+ SS+ SKW GD+EKL K LF A
Sbjct: 93 LLRPCKGILLFGPPGTGKTLLAKALATEAGANFISITGSSLTSKWFGDAEKLTKALFSFA 272
Query: 201 RHHAPSTIFLDEIDAIISQRGEARSEHEASR 293
AP IF DE+D+++ RG A R
Sbjct: 273 SRLAPVIIFXDEVDSLLGARGGAMEHEATRR 365
>gb|BE456133.3|BE456133 HVSMEg0019P07f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0019P07f, mRNA sequence
Length = 592
Score = 119 bits (255), Expect = 3e-026
Identities = 48/51 (94%), Positives = 48/51 (94%)
Frame = +3 / +2
Query: 3 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKW 155
PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATEC TTFFNISA IVSKW
Sbjct: 401 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECNTTFFNISALLIVSKW 553
Score = 108 bits (231), Expect = 5e-023
Identities = 44/57 (77%), Positives = 48/57 (84%)
Frame = -1 / -2
Query: 172 FSESPRHLLTIDDAEMLKKVVLHSVATAFASIVFPVPGGPKSKMPFHGERRPVKYFG 2
F HLLTI+ AEMLK VVLHSVATA A++VFPVPGGPKSKMPFHG+ RPVKYFG
Sbjct: 570 FPPKTAHLLTINKAEMLKNVVLHSVATALANMVFPVPGGPKSKMPFHGDSRPVKYFG 400
>gb|BQ768557.1|BQ768557 EBro08_SQ012_E03_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ012_E03 5', mRNA sequence
Length = 737
Score = 117 bits (251), Expect = 9e-026
Identities = 49/114 (42%), Positives = 73/114 (64%)
Frame = +3 / +2
Query: 36 KGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELARHHAP 215
KG+LL+GPPGTGKT+LA+AVA TF +S S +V K+ G+ ++V+ LF +AR HAP
Sbjct: 143 KGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGARMVRELFVMARQHAP 322
Query: 216 STIFLDEIDAIISQRGEARSEHEASRRLKTELLIQMDGLTKTNDLVFVLAATNL 377
+ IF+DE+D+I S R E+ +R ELL Q+DG ++ ++A +
Sbjct: 323 TIIFMDEVDSIGSTRIESSGGDSEVQRTMMELLNQLDGFEAAKNIKVLMATNRI 484
>gb|CA022858.1|CA022858 HZ44I22r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ44I22
5-PRIME, mRNA sequence
Length = 563
Score = 117 bits (251), Expect = 9e-026
Identities = 48/49 (97%), Positives = 48/49 (97%)
Frame = +3 / +3
Query: 3 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVS 149
PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATEC TTFFNISASSIVS
Sbjct: 417 PKYFTGLLSPWKGILLFGPPGTGKTMLAKAVATECNTTFFNISASSIVS 563
Score = 105 bits (225), Expect = 3e-022
Identities = 42/49 (85%), Positives = 46/49 (93%)
Frame = -1 / -2
Query: 148 LTIDDAEMLKKVVLHSVATAFASIVFPVPGGPKSKMPFHGERRPVKYFG 2
LTID+AEMLK VVLHSVATA A++VFPVPGGPKSKMPFHG+ RPVKYFG
Sbjct: 562 LTIDEAEMLKNVVLHSVATALANMVFPVPGGPKSKMPFHGDSRPVKYFG 416
>gb|BU988151.1|BU988151 HF16M22r HF Hordeum vulgare subsp. vulgare cDNA clone HF16M22
5-PRIME, mRNA sequence
Length = 679
Score = 91.3 bits (193), Expect(2) = 6e-025
Identities = 39/101 (38%), Positives = 60/101 (59%)
Frame = +3 / +1
Query: 63 GTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEID 242
G KT++A+AVA+E K F + + SKW GDSEK V+ LF A+ +AP+ +F DEID
Sbjct: 1 GCSKTLMARAVASEAKMNFLAVKGPELFSKWVGDSEKAVRSLFAKAKENAPAILFFDEID 180
Query: 243 AIISQRGEARSEHEASRRLKTELLIQMDGLTKTNDLVFVLA 365
+ RG + + R+ ++LL +MDGL + + + A
Sbjct: 181 GLAVTRGHGSNGISVADRVLSQLLQEMDGLDQRIGVTVIAA 303
Score = 43.2 bits (88), Expect(2) = 6e-025
Identities = 21/72 (29%), Positives = 38/72 (52%)
Frame = +3 / +1
Query: 408 RLEKRILVPLPEGEARQAMFEELLPATTSKLEIPYDILVEKTEGYSGSDIRLVCKEAAMQ 587
R ++ + V P+ R+ +F + ++ + L TEGY+G+DI+LVC+EAA+
Sbjct: 349 RFDRLLDVQPPDEADREDIFRIHTRSIPCSDDVNLNELARLTEGYTGADIKLVCREAAVA 528
Query: 588 PLRRLMSVLEAS 623
L + E +
Sbjct: 529 ALDESFDIPEVA 564
>gb|BF622331.2|BF622331 HVSMEa0002M16f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0002M16f, mRNA sequence
Length = 853
Score = 85.8 bits (181), Expect(3) = 1e-024
Identities = 36/91 (39%), Positives = 57/91 (62%)
Frame = +3 / +3
Query: 333 TKTNDLVFVLAATNLPWELDAAMLRRLEKRILVPLPEGEARQAMFEELLPATTSKLEIPY 512
+K + + +L ATN P++LD A++RRL +RI V LP+ + R + + LL + E +
Sbjct: 159 SKDSQRILILGATNRPFDLDDAVIRRLPRRIYVDLPDAQNRMKILKILLAKENLESEFGF 338
Query: 513 DILVEKTEGYSGSDIRLVCKEAAMQPLRRLM 605
D L TEGYSGSD++ +C AA +P+ L+
Sbjct: 339 DELANATEGYSGSDLKNLCVAAAYRPVHELL 431
Score = 36.3 bits (73), Expect(3) = 1e-024
Identities = 15/38 (39%), Positives = 20/38 (52%)
Frame = +3 / +3
Query: 180 KVLFELARHHAPSTIFLDEIDAIISQRGEARSEHEASR 293
K LF A AP IF+DE+D+++ RG A R
Sbjct: 6 KALFSFASRLAPVIIFVDEVDSLLGARGGAMEHEATRR 119
Score = 29.0 bits (57), Expect(3) = 1e-024
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +3 / +3
Query: 276 EHEASRRLKTELLIQMDGL 332
EHEA+RR++ E + DGL
Sbjct: 99 EHEATRRMRNEFMAAWDGL 155
>gb|BQ767779.1|BQ767779 EBro08_SQ009_G20_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ009_G20 5', mRNA sequence
Length = 551
Score = 79.9 bits (168), Expect(3) = 2e-024
Identities = 30/67 (44%), Positives = 46/67 (68%)
Frame = +3 / +3
Query: 69 GKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELARHHAPSTIFLDEIDAI 248
GKT+LAKA+A EC+ F +I +++ W G+SE V+ +F+ AR AP +F DE+D+I
Sbjct: 6 GKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARGSAPCVLFFDELDSI 185
Query: 249 ISQRGEA 269
+QRG +
Sbjct: 186 ATQRGSS 206
Score = 46.4 bits (95), Expect(3) = 2e-024
Identities = 19/63 (30%), Positives = 36/63 (57%)
Frame = +3 / +3
Query: 408 RLEKRILVPLPEGEARQAMFEELLPATTSKLEIPYDILVEKTEGYSGSDIRLVCKEAAMQ 587
RL++ I +PLP+ ++R +F+ L + +I L + T+ +SG+DI +C+ A
Sbjct: 351 RLDQLIYIPLPDVDSRHQIFKACLKKSPLAKDIDLSALAKYTQRFSGADITEICQRACKY 530
Query: 588 PLR 596
+R
Sbjct: 531 AIR 539
Score = 24.4 bits (47), Expect(3) = 2e-024
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = +3 / +3
Query: 351 VFVLAATNLPWELDAAMLR 407
VF++ ATN P +D A+L+
Sbjct: 288 VFIIGATNRPDIIDPALLK 344
>gb|AV926794.1|AV926794 AV926794 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd23m18 5', mRNA sequence
Length = 661
Score = 105 bits (224), Expect(2) = 3e-024
Identities = 39/82 (47%), Positives = 58/82 (70%)
Frame = +3 / +2
Query: 24 LSPWKGILLFGPPGTGKTMLAKAVATECKTTFFNISASSIVSKWRGDSEKLVKVLFELAR 203
+SP KG+L +GPPG GKT+LAKA+A EC+ F +I +++ W G+SE V+ +F+ AR
Sbjct: 212 MSPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKAR 391
Query: 204 HHAPSTIFLDEIDAIISQRGEA 269
AP +F DE+D+I +QRG +
Sbjct: 392 GSAPCVLFFDELDSIATQRGSS 457
Score = 26.7 bits (52), Expect(2) = 3e-024
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +3 / +2
Query: 351 VFVLAATNLPWELDAAMLR 407
VF++ ATN P +D A+LR
Sbjct: 539 VFIIGATNRPDXIDPALLR 595
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 558,636,021
Number of Sequences: 312970
Number of extensions: 8312124
Number of successful extensions: 44457
Number of sequences better than 1.0e-020: 139
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 14083489153
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)