TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2404863.2.1
(672 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV925892.1|AV925892 AV925892 K. Sato unpublished cDNA li... 205 2e-052
gb|AV928662.1|AV928662 AV928662 K. Sato unpublished cDNA li... 205 2e-052
gb|AV935887.1|AV935887 AV935887 K. Sato unpublished cDNA li... 205 2e-052
gb|BU976140.1|BU976140 HA03D09u HA Hordeum vulgare subsp. v... 205 2e-052
gb|CV055547.1|CV055547 BNEL120h12 Barley EST endosperm libr... 188 3e-047
gb|CV059284.1|CV059284 BNEL46d8 Barley EST endosperm librar... 186 1e-046
gb|CV062825.1|CV062825 BNEL83e1 Barley EST endosperm librar... 183 9e-046
gb|CA031834.1|CA031834 HX11E06r HX Hordeum vulgare subsp. v... 168 3e-041
gb|BQ657408.1|BQ657408 HA08H22u HA Hordeum vulgare subsp. v... 148 3e-035
gb|BI777049.2|BI777049 EBpi03_SQ001_M01_R pistil, 4 DPA, no... 85 5e-034
gb|CV059060.1|CV059060 BNEL43h6 Barley EST endosperm librar... 141 4e-033
gb|BQ760241.1|BQ760241 EBpi07_SQ003_K16_R pistil, 12 DPA, n... 75 6e-028
gb|BU985075.1|BU985075 HF06A23r HF Hordeum vulgare subsp. v... 122 3e-027
gb|CV056920.1|CV056920 BNEL22a9 Barley EST endosperm librar... 122 3e-027
gb|CV055258.1|CV055258 BNEL118f1 Barley EST endosperm libra... 111 5e-024
>gb|AV925892.1|AV925892 AV925892 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basdd19 5', mRNA sequence
Length = 612
Score = 205 bits (442), Expect = 2e-052
Identities = 85/87 (97%), Positives = 85/87 (97%)
Frame = +1 / +1
Query: 1 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHGGD 180
GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVH GD
Sbjct: 316 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHVGD 495
Query: 181 EVYSNGGVVLPQKEIKSSILKPEIVM* 261
EVYSNGGVVLP KEIKSSILKPEIVM*
Sbjct: 496 EVYSNGGVVLPHKEIKSSILKPEIVM* 576
Score = 134 bits (288), Expect = 4e-031
Identities = 53/86 (61%), Positives = 64/86 (74%)
Frame = +3 / +3
Query: 3 TWMCRGGWREAFPLHCHAWCAYQEACLHLEQHYRVALNCWTMGTDREYDHPGRGCSWG*R 182
TWM GGW EA P+H AWC + EAC+HL+QH+R+A + MGT REYD+PGR C+ *R
Sbjct: 318 TWMRCGGWCEALPMHGDAWCTH*EACVHLKQHHRLAFDGRAMGTHREYDYPGRRCTCW*R 497
Query: 183 GIQQWRCCSPTERDQVKHFEA*DRHV 260
G+QQ RCCS + DQVKH A*DRHV
Sbjct: 498 GLQQRRCCSAAQGDQVKHP*A*DRHV 575
Score = 126 bits (269), Expect = 2e-028
Identities = 54/87 (62%), Positives = 68/87 (78%)
Frame = -1 / -1
Query: 261 SHDDLRLQNA*LDLFLWENNTAIAVYLVTPMNILAQDGHILYPCPLSNS*VPPDNAVRDA 82
SHDDLRL++A*LDL + +NNTA+AV LVT M I +QD HILY CPL + +P D+AV DA
Sbjct: 576 SHDDLRLKDA*LDLLVRQNNTAVAVDLVTNMYIFSQDSHILYACPLPDRRMPADDAV*DA 397
Query: 81 GMLLDTHTTHDSAAGKPHAILHDTSRS 1
MLL+ +TTH A+G+PH ILH+ SRS
Sbjct: 396 RMLLNAYTTHHRASGEPHTILHNASRS 316
Score = 112 bits (239), Expect(2) = 1e-030
Identities = 43/51 (84%), Positives = 48/51 (94%)
Frame = -2 / -2
Query: 155 RMVIFSIRAHCPTVECHPIMLFEMQACFLIRTPRMTVQRESLTPSSTTHPG 3
R+VIFS+RAHCPTVEC P+MLFEM ACFL+RTPR+TV RESLTPSSTTHPG
Sbjct: 470 RIVIFSMRAHCPTVECQPMMLFEMHACFLMRTPRITVHRESLTPSSTTHPG 318
Score = 108 bits (231), Expect = 3e-023
Identities = 54/87 (62%), Positives = 59/87 (67%)
Frame = +2 / +2
Query: 2 DLDVSWRMA*GFPAALSCVVCVSRSMPASRTALSGGTQLLDNGHG*RI*PSWARMFMGVT 181
DLD WRM *G P A CVV RSM AS+TA S G + NGH *RI* SW +M+M VT
Sbjct: 317 DLDALWRMV*GSPDAR*CVVYALRSMRASQTASSAGIRRSGNGHA*RI*LSWEKMYMLVT 496
Query: 182 RYTAMAVLFSHRKRSSQAF*SLRSSCE 262
R TA AVLF +RSSQA SLRSSCE
Sbjct: 497 RSTATAVLFCRTRRSSQASLSLRSSCE 577
Score = 40.5 bits (82), Expect(2) = 1e-030
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = -2 / -2
Query: 263 SHMTISGFKMLDLISFCGR 207
+HMTISG +MLDLIS CGR
Sbjct: 578 AHMTISGLRMLDLISLCGR 522
>gb|AV928662.1|AV928662 AV928662 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd15d19 3', mRNA sequence
Length = 673
Score = 205 bits (442), Expect = 2e-052
Identities = 85/87 (97%), Positives = 85/87 (97%)
Frame = +1 / -1
Query: 1 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHGGD 180
GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVH GD
Sbjct: 367 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHVGD 188
Query: 181 EVYSNGGVVLPQKEIKSSILKPEIVM* 261
EVYSNGGVVLP KEIKSSILKPEIVM*
Sbjct: 187 EVYSNGGVVLPHKEIKSSILKPEIVM* 107
Score = 134 bits (288), Expect = 4e-031
Identities = 53/86 (61%), Positives = 64/86 (74%)
Frame = +3 / -3
Query: 3 TWMCRGGWREAFPLHCHAWCAYQEACLHLEQHYRVALNCWTMGTDREYDHPGRGCSWG*R 182
TWM GGW EA P+H AWC + EAC+HL+QH+R+A + MGT REYD+PGR C+ *R
Sbjct: 365 TWMRCGGWCEALPMHGDAWCTH*EACVHLKQHHRLAFDGRAMGTHREYDYPGRRCTCW*R 186
Query: 183 GIQQWRCCSPTERDQVKHFEA*DRHV 260
G+QQ RCCS + DQVKH A*DRHV
Sbjct: 185 GLQQRRCCSAAQGDQVKHP*A*DRHV 108
Score = 126 bits (269), Expect = 2e-028
Identities = 54/87 (62%), Positives = 68/87 (78%)
Frame = -1 / +2
Query: 261 SHDDLRLQNA*LDLFLWENNTAIAVYLVTPMNILAQDGHILYPCPLSNS*VPPDNAVRDA 82
SHDDLRL++A*LDL + +NNTA+AV LVT M I +QD HILY CPL + +P D+AV DA
Sbjct: 107 SHDDLRLKDA*LDLLVRQNNTAVAVDLVTNMYIFSQDSHILYACPLPDRRMPADDAV*DA 286
Query: 81 GMLLDTHTTHDSAAGKPHAILHDTSRS 1
MLL+ +TTH A+G+PH ILH+ SRS
Sbjct: 287 RMLLNAYTTHHRASGEPHTILHNASRS 367
Score = 112 bits (239), Expect(2) = 1e-030
Identities = 43/51 (84%), Positives = 48/51 (94%)
Frame = -2 / +3
Query: 155 RMVIFSIRAHCPTVECHPIMLFEMQACFLIRTPRMTVQRESLTPSSTTHPG 3
R+VIFS+RAHCPTVEC P+MLFEM ACFL+RTPR+TV RESLTPSSTTHPG
Sbjct: 213 RIVIFSMRAHCPTVECQPMMLFEMHACFLMRTPRITVHRESLTPSSTTHPG 365
Score = 108 bits (231), Expect = 3e-023
Identities = 54/87 (62%), Positives = 59/87 (67%)
Frame = +2 / -2
Query: 2 DLDVSWRMA*GFPAALSCVVCVSRSMPASRTALSGGTQLLDNGHG*RI*PSWARMFMGVT 181
DLD WRM *G P A CVV RSM AS+TA S G + NGH *RI* SW +M+M VT
Sbjct: 366 DLDALWRMV*GSPDAR*CVVYALRSMRASQTASSAGIRRSGNGHA*RI*LSWEKMYMLVT 187
Query: 182 RYTAMAVLFSHRKRSSQAF*SLRSSCE 262
R TA AVLF +RSSQA SLRSSCE
Sbjct: 186 RSTATAVLFCRTRRSSQASLSLRSSCE 106
Score = 40.5 bits (82), Expect(2) = 1e-030
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = -2 / +3
Query: 263 SHMTISGFKMLDLISFCGR 207
+HMTISG +MLDLIS CGR
Sbjct: 105 AHMTISGLRMLDLISLCGR 161
>gb|AV935887.1|AV935887 AV935887 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal6n22 3', mRNA sequence
Length = 651
Score = 205 bits (442), Expect = 2e-052
Identities = 85/87 (97%), Positives = 85/87 (97%)
Frame = +1 / -2
Query: 1 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHGGD 180
GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVH GD
Sbjct: 335 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHVGD 156
Query: 181 EVYSNGGVVLPQKEIKSSILKPEIVM* 261
EVYSNGGVVLP KEIKSSILKPEIVM*
Sbjct: 155 EVYSNGGVVLPHKEIKSSILKPEIVM* 75
Score = 134 bits (288), Expect = 4e-031
Identities = 53/86 (61%), Positives = 64/86 (74%)
Frame = +3 / -1
Query: 3 TWMCRGGWREAFPLHCHAWCAYQEACLHLEQHYRVALNCWTMGTDREYDHPGRGCSWG*R 182
TWM GGW EA P+H AWC + EAC+HL+QH+R+A + MGT REYD+PGR C+ *R
Sbjct: 333 TWMRCGGWCEALPMHGDAWCTH*EACVHLKQHHRLAFDGRAMGTHREYDYPGRRCTCW*R 154
Query: 183 GIQQWRCCSPTERDQVKHFEA*DRHV 260
G+QQ RCCS + DQVKH A*DRHV
Sbjct: 153 GLQQRRCCSAAQGDQVKHP*A*DRHV 76
Score = 126 bits (269), Expect = 2e-028
Identities = 54/87 (62%), Positives = 68/87 (78%)
Frame = -1 / +3
Query: 261 SHDDLRLQNA*LDLFLWENNTAIAVYLVTPMNILAQDGHILYPCPLSNS*VPPDNAVRDA 82
SHDDLRL++A*LDL + +NNTA+AV LVT M I +QD HILY CPL + +P D+AV DA
Sbjct: 75 SHDDLRLKDA*LDLLVRQNNTAVAVDLVTNMYIFSQDSHILYACPLPDRRMPADDAV*DA 254
Query: 81 GMLLDTHTTHDSAAGKPHAILHDTSRS 1
MLL+ +TTH A+G+PH ILH+ SRS
Sbjct: 255 RMLLNAYTTHHRASGEPHTILHNASRS 335
Score = 112 bits (239), Expect(2) = 1e-030
Identities = 43/51 (84%), Positives = 48/51 (94%)
Frame = -2 / +1
Query: 155 RMVIFSIRAHCPTVECHPIMLFEMQACFLIRTPRMTVQRESLTPSSTTHPG 3
R+VIFS+RAHCPTVEC P+MLFEM ACFL+RTPR+TV RESLTPSSTTHPG
Sbjct: 181 RIVIFSMRAHCPTVECQPMMLFEMHACFLMRTPRITVHRESLTPSSTTHPG 333
Score = 108 bits (231), Expect = 3e-023
Identities = 54/87 (62%), Positives = 59/87 (67%)
Frame = +2 / -3
Query: 2 DLDVSWRMA*GFPAALSCVVCVSRSMPASRTALSGGTQLLDNGHG*RI*PSWARMFMGVT 181
DLD WRM *G P A CVV RSM AS+TA S G + NGH *RI* SW +M+M VT
Sbjct: 334 DLDALWRMV*GSPDAR*CVVYALRSMRASQTASSAGIRRSGNGHA*RI*LSWEKMYMLVT 155
Query: 182 RYTAMAVLFSHRKRSSQAF*SLRSSCE 262
R TA AVLF +RSSQA SLRSSCE
Sbjct: 154 RSTATAVLFCRTRRSSQASLSLRSSCE 74
Score = 40.5 bits (82), Expect(2) = 1e-030
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = -2 / +1
Query: 263 SHMTISGFKMLDLISFCGR 207
+HMTISG +MLDLIS CGR
Sbjct: 73 AHMTISGLRMLDLISLCGR 129
>gb|BU976140.1|BU976140 HA03D09u HA Hordeum vulgare subsp. vulgare cDNA clone HA03D09
3-PRIME, mRNA sequence
Length = 485
Score = 205 bits (442), Expect = 2e-052
Identities = 85/87 (97%), Positives = 85/87 (97%)
Frame = +1 / -1
Query: 1 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHGGD 180
GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVH GD
Sbjct: 440 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHVGD 261
Query: 181 EVYSNGGVVLPQKEIKSSILKPEIVM* 261
EVYSNGGVVLP KEIKSSILKPEIVM*
Sbjct: 260 EVYSNGGVVLPHKEIKSSILKPEIVM* 180
Score = 134 bits (288), Expect = 4e-031
Identities = 53/86 (61%), Positives = 64/86 (74%)
Frame = +3 / -3
Query: 3 TWMCRGGWREAFPLHCHAWCAYQEACLHLEQHYRVALNCWTMGTDREYDHPGRGCSWG*R 182
TWM GGW EA P+H AWC + EAC+HL+QH+R+A + MGT REYD+PGR C+ *R
Sbjct: 438 TWMRCGGWCEALPMHGDAWCTH*EACVHLKQHHRLAFDGRAMGTHREYDYPGRRCTCW*R 259
Query: 183 GIQQWRCCSPTERDQVKHFEA*DRHV 260
G+QQ RCCS + DQVKH A*DRHV
Sbjct: 258 GLQQRRCCSAAQGDQVKHP*A*DRHV 181
Score = 126 bits (269), Expect = 2e-028
Identities = 54/87 (62%), Positives = 68/87 (78%)
Frame = -1 / +3
Query: 261 SHDDLRLQNA*LDLFLWENNTAIAVYLVTPMNILAQDGHILYPCPLSNS*VPPDNAVRDA 82
SHDDLRL++A*LDL + +NNTA+AV LVT M I +QD HILY CPL + +P D+AV DA
Sbjct: 180 SHDDLRLKDA*LDLLVRQNNTAVAVDLVTNMYIFSQDSHILYACPLPDRRMPADDAV*DA 359
Query: 81 GMLLDTHTTHDSAAGKPHAILHDTSRS 1
MLL+ +TTH A+G+PH ILH+ SRS
Sbjct: 360 RMLLNAYTTHHRASGEPHTILHNASRS 440
Score = 112 bits (239), Expect(2) = 1e-030
Identities = 43/51 (84%), Positives = 48/51 (94%)
Frame = -2 / +1
Query: 155 RMVIFSIRAHCPTVECHPIMLFEMQACFLIRTPRMTVQRESLTPSSTTHPG 3
R+VIFS+RAHCPTVEC P+MLFEM ACFL+RTPR+TV RESLTPSSTTHPG
Sbjct: 286 RIVIFSMRAHCPTVECQPMMLFEMHACFLMRTPRITVHRESLTPSSTTHPG 438
Score = 108 bits (231), Expect = 3e-023
Identities = 54/87 (62%), Positives = 59/87 (67%)
Frame = +2 / -2
Query: 2 DLDVSWRMA*GFPAALSCVVCVSRSMPASRTALSGGTQLLDNGHG*RI*PSWARMFMGVT 181
DLD WRM *G P A CVV RSM AS+TA S G + NGH *RI* SW +M+M VT
Sbjct: 439 DLDALWRMV*GSPDAR*CVVYALRSMRASQTASSAGIRRSGNGHA*RI*LSWEKMYMLVT 260
Query: 182 RYTAMAVLFSHRKRSSQAF*SLRSSCE 262
R TA AVLF +RSSQA SLRSSCE
Sbjct: 259 RSTATAVLFCRTRRSSQASLSLRSSCE 179
Score = 40.5 bits (82), Expect(2) = 1e-030
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = -2 / +1
Query: 263 SHMTISGFKMLDLISFCGR 207
+HMTISG +MLDLIS CGR
Sbjct: 178 AHMTISGLRMLDLISLCGR 234
>gb|CV055547.1|CV055547 BNEL120h12 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL120h12 5' similar to GDP-mannose
pyrophosphorylase, mRNA sequence
Length = 730
Score = 188 bits (405), Expect = 3e-047
Identities = 76/87 (87%), Positives = 81/87 (93%)
Frame = +1 / -3
Query: 1 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHGGD 180
GPGCVV+ GVRLSRCTVMRG R+KKHACIS SI+GWHSTVG+WAR+ENMTILGEDVH D
Sbjct: 569 GPGCVVKPGVRLSRCTVMRGARVKKHACISGSIVGWHSTVGKWARVENMTILGEDVHVCD 390
Query: 181 EVYSNGGVVLPQKEIKSSILKPEIVM* 261
EVYSNGGVVLP KEIKSSILKPEIVM*
Sbjct: 389 EVYSNGGVVLPHKEIKSSILKPEIVM* 309
Score = 115 bits (246), Expect = 2e-025
Identities = 49/87 (56%), Positives = 54/87 (62%)
Frame = -2 / +1
Query: 263 SHMTISGFKMLDLISFCGRXXXXXXXXXXXXXXXXPRMVIFSIRAHCPTVECHPIMLFEM 84
+HMTISG +MLDLIS CG PRMV+FS RAH PTVECHP ML EM
Sbjct: 307 THMTISGLRMLDLISLCGSTTPPLLYTSSQTCTSSPRMVMFSTRAHLPTVECHPTMLPEM 486
Query: 83 QACFLIRTPRMTVQRESLTPSSTTHPG 3
ACF R PR+TV + TP TTHPG
Sbjct: 487 HACFFTRAPRITVHLDRRTPGFTTHPG 567
>gb|CV059284.1|CV059284 BNEL46d8 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL46d8 5' similar to COG1208, GCD1,
Nucleoside-diphosphate-sugar pyrophosphorylase involved
in lipopolysaccharide biosynthesis/translation
initiation factor 2B, gamma/epsilon subunits, mRNA
sequence
Length = 637
Score = 186 bits (400), Expect = 1e-046
Identities = 76/87 (87%), Positives = 80/87 (91%)
Frame = +1 / +2
Query: 1 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHGGD 180
GP CVVE GVRLSRCTVMRG R+KKHACIS SI+GWHSTVG+WAR+ENMTILGEDVH D
Sbjct: 365 GPRCVVEAGVRLSRCTVMRGARVKKHACISGSIVGWHSTVGKWARVENMTILGEDVHVCD 544
Query: 181 EVYSNGGVVLPQKEIKSSILKPEIVM* 261
EVYSNGGVVLP KEIKSSILKPEIVM*
Sbjct: 545 EVYSNGGVVLPHKEIKSSILKPEIVM* 625
Score = 113 bits (241), Expect = 1e-024
Identities = 49/87 (56%), Positives = 55/87 (63%)
Frame = -2 / -2
Query: 263 SHMTISGFKMLDLISFCGRXXXXXXXXXXXXXXXXPRMVIFSIRAHCPTVECHPIMLFEM 84
+HMTISG +MLDLIS CG PRMV+FS RAH PTVECHP ML EM
Sbjct: 627 THMTISGLRMLDLISLCGSTTPPLLYTSSQTCTSSPRMVMFSTRAHLPTVECHPTMLPEM 448
Query: 83 QACFLIRTPRMTVQRESLTPSSTTHPG 3
ACF R PR+TV +S TP+STT G
Sbjct: 447 HACFFTRAPRITVHLDSRTPASTTQRG 367
>gb|CV062825.1|CV062825 BNEL83e1 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL83e1 5' similar to GDP-mannose
pyrophosphorylase, mRNA sequence
Length = 595
Score = 183 bits (394), Expect = 9e-046
Identities = 75/87 (86%), Positives = 79/87 (90%)
Frame = +1 / -3
Query: 1 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHGGD 180
G GCVV GVRLSRCTVMRG R+KKHACIS SI+GWHSTVG+WAR+ENMTILGEDVH D
Sbjct: 560 GTGCVVXAGVRLSRCTVMRGARVKKHACISGSIVGWHSTVGKWARVENMTILGEDVHVCD 381
Query: 181 EVYSNGGVVLPQKEIKSSILKPEIVM* 261
EVYSNGGVVLP KEIKSSILKPEIVM*
Sbjct: 380 EVYSNGGVVLPHKEIKSSILKPEIVM* 300
Score = 116 bits (248), Expect = 1e-025
Identities = 49/86 (56%), Positives = 55/86 (63%)
Frame = -2 / +1
Query: 263 SHMTISGFKMLDLISFCGRXXXXXXXXXXXXXXXXPRMVIFSIRAHCPTVECHPIMLFEM 84
+HMTISG +MLDLIS CG PRMV+FS RAH PTVECHP ML EM
Sbjct: 298 THMTISGLRMLDLISLCGSTTPPLLYTSSQTCTSSPRMVMFSTRAHLPTVECHPTMLPEM 477
Query: 83 QACFLIRTPRMTVQRESLTPSSTTHP 6
ACF R PR+TV +S TP+ TTHP
Sbjct: 478 HACFFTRAPRITVHLDSRTPAXTTHP 555
>gb|CA031834.1|CA031834 HX11E06r HX Hordeum vulgare subsp. vulgare cDNA clone HX11E06
5-PRIME, mRNA sequence
Length = 620
Score = 168 bits (361), Expect = 3e-041
Identities = 66/76 (86%), Positives = 70/76 (92%)
Frame = +1 / +3
Query: 1 GPGCVVEDGVRLSRCTVMRGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHGGD 180
GPGCVVE GVRLSRCTVMRG R+KKHACIS SI+GWHSTVG+WAR+ENMTILGEDVH D
Sbjct: 393 GPGCVVEAGVRLSRCTVMRGARVKKHACISGSIVGWHSTVGKWARVENMTILGEDVHVCD 572
Query: 181 EVYSNGGVVLPQKEIK 228
EVYSNGGVVLP KEIK
Sbjct: 573 EVYSNGGVVLPHKEIK 620
>gb|BQ657408.1|BQ657408 HA08H22u HA Hordeum vulgare subsp. vulgare cDNA clone HA08H22
3-PRIME, mRNA sequence
Length = 497
Score = 148 bits (318), Expect = 3e-035
Identities = 60/69 (86%), Positives = 64/69 (92%)
Frame = +1 / -2
Query: 55 RGVRIKKHACISNSIIGWHSTVGQWARIENMTILGEDVHGGDEVYSNGGVVLPQKEIKSS 234
RG R+KKHACIS SI+GWHSTVG+WAR+ENMTILGEDVH DEVYSNGGVVLP KEIKSS
Sbjct: 496 RGARVKKHACISGSIVGWHSTVGKWARVENMTILGEDVHVCDEVYSNGGVVLPHKEIKSS 317
Query: 235 ILKPEIVM* 261
ILKPEIVM*
Sbjct: 316 ILKPEIVM* 290
>gb|BI777049.2|BI777049 EBpi03_SQ001_M01_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ001_M01 5', mRNA sequence
Length = 245
Score = 84.9 bits (179), Expect(2) = 5e-034
Identities = 37/39 (94%), Positives = 37/39 (94%)
Frame = +1 / +3
Query: 145 MTILGEDVHGGDEVYSNGGVVLPQKEIKSSILKPEIVM* 261
MTILGEDVH GDEVYSNGGVVLP KEIKSSILKPEIVM*
Sbjct: 96 MTILGEDVHVGDEVYSNGGVVLPHKEIKSSILKPEIVM* 212
Score = 78.5 bits (165), Expect(2) = 5e-034
Identities = 30/32 (93%), Positives = 31/32 (96%)
Frame = +1 / +2
Query: 49 VMRGVRIKKHACISNSIIGWHSTVGQWARIEN 144
VMRGVRIKKHACI +SIIGWHSTVGQWARIEN
Sbjct: 2 VMRGVRIKKHACIGDSIIGWHSTVGQWARIEN 97
Score = 66.1 bits (138), Expect(2) = 2e-023
Identities = 24/32 (75%), Positives = 28/32 (87%)
Frame = -2 / -3
Query: 143 FSIRAHCPTVECHPIMLFEMQACFLIRTPRMT 48
FS+RAHCPTVEC P+ML M ACFL+RTPR+T
Sbjct: 96 FSMRAHCPTVECQPMMLSPMHACFLMRTPRIT 1
Score = 62.0 bits (129), Expect(2) = 2e-023
Identities = 27/42 (64%), Positives = 29/42 (69%)
Frame = -3 / -3
Query: 262 LT*RSQASKCLT*SLSVGEQHRHCCIPRHPHEHPRPGWSYSL 137
LT*RSQA CLT*S EQHR CC PRH H H PG S+S+
Sbjct: 213 LT*RSQA*GCLT*SPCAAEQHRRCCRPRHQHVHLLPG*SFSM 88
>gb|CV059060.1|CV059060 BNEL43h6 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL43h6 5' similar to GDP-mannose
pyrophosphorylase, mRNA sequence
Length = 500
Score = 141 bits (302), Expect = 4e-033
Identities = 57/65 (87%), Positives = 61/65 (93%)
Frame = +1 / -3
Query: 67 IKKHACISNSIIGWHSTVGQWARIENMTILGEDVHGGDEVYSNGGVVLPQKEIKSSILKP 246
+KKHACIS SI+GWHSTVG+WAR+ENMTILGEDVH DEVYSNGGVVLP KEIKSSILKP
Sbjct: 498 VKKHACISGSIVGWHSTVGKWARVENMTILGEDVHVCDEVYSNGGVVLPHKEIKSSILKP 319
Query: 247 EIVM* 261
EIVM*
Sbjct: 318 EIVM* 304
>gb|BQ760241.1|BQ760241 EBpi07_SQ003_K16_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ003_K16 5', mRNA sequence
Length = 357
Score = 74.8 bits (157), Expect(2) = 6e-028
Identities = 32/35 (91%), Positives = 33/35 (94%)
Frame = +1 / +1
Query: 157 GEDVHGGDEVYSNGGVVLPQKEIKSSILKPEIVM* 261
G+DVH GDEVYSNGGVVLP KEIKSSILKPEIVM*
Sbjct: 76 GKDVHVGDEVYSNGGVVLPHKEIKSSILKPEIVM* 180
Score = 68.9 bits (144), Expect(2) = 6e-028
Identities = 26/27 (96%), Positives = 27/27 (100%)
Frame = +1 / +2
Query: 82 CISNSIIGWHSTVGQWARIENMTILGE 162
CISNSIIGWHSTVGQWARIENMTILG+
Sbjct: 2 CISNSIIGWHSTVGQWARIENMTILGK 82
>gb|BU985075.1|BU985075 HF06A23r HF Hordeum vulgare subsp. vulgare cDNA clone HF06A23
5-PRIME, mRNA sequence
Length = 455
Score = 122 bits (260), Expect = 3e-027
Identities = 50/56 (89%), Positives = 53/56 (94%)
Frame = +1 / -1
Query: 94 SIIGWHSTVGQWARIENMTILGEDVHGGDEVYSNGGVVLPQKEIKSSILKPEIVM* 261
SI+GWHSTVG+WAR+ENMTILGEDVH DEVYSNGGVVLP KEIKSSILKPEIVM*
Sbjct: 455 SIVGWHSTVGKWARVENMTILGEDVHVCDEVYSNGGVVLPHKEIKSSILKPEIVM* 288
>gb|CV056920.1|CV056920 BNEL22a9 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL22a9 5' similar to GDP-D-mannose
pyrophosphorylase, mRNA sequence
Length = 555
Score = 122 bits (260), Expect = 3e-027
Identities = 50/56 (89%), Positives = 53/56 (94%)
Frame = +1 / -2
Query: 94 SIIGWHSTVGQWARIENMTILGEDVHGGDEVYSNGGVVLPQKEIKSSILKPEIVM* 261
SI+GWHSTVG+WAR+ENMTILGEDVH DEVYSNGGVVLP KEIKSSILKPEIVM*
Sbjct: 476 SIVGWHSTVGKWARVENMTILGEDVHVCDEVYSNGGVVLPHKEIKSSILKPEIVM* 309
>gb|CV055258.1|CV055258 BNEL118f1 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL118f1 5' similar to COG1208,
GCD1, Nucleoside-diphosphate-sugar pyrophosphorylase
involved in lipopolysaccharide biosynthesis/translation
initiation factor 2B, gamma/epsilon subunits, mRNA
sequence
Length = 778
Score = 111 bits (236), Expect = 5e-024
Identities = 46/64 (71%), Positives = 52/64 (81%)
Frame = +1 / -1
Query: 70 KKHACISNSIIGWHSTVGQWARIENMTILGEDVHGGDEVYSNGGVVLPQKEIKSSILKPE 249
+ HACIS+SI+GWH V +WAR+ENMTI GEDVH DEVY GV LP KEI+SSILKPE
Sbjct: 511 RSHACISDSIVGWHFAVDKWARVENMTIQGEDVHVFDEVYIIAGVGLPHKEIESSILKPE 332
Query: 250 IVM* 261
IVM*
Sbjct: 331 IVM* 320
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 323,521,765
Number of Sequences: 312970
Number of extensions: 4585451
Number of successful extensions: 43177
Number of sequences better than 1.0e-020: 21
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7295673948
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)