TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192679.2.1
(1274 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE602597.3|BE602597 HVSMEh0100E21f Hordeum vulgare 5-45 ... 296 2e-081
gb|AL507344.1|AL507344 AL507344 Hordeum vulgare Barke devel... 230 4e-063
gb|BI776636.2|BI776636 EBpi07_SQ001_D15_R pistil, 12 DPA, n... 160 2e-062
gb|BQ764915.1|BQ764915 EBca01_SQ005_B12_R carpel, pre-anthe... 133 2e-035
gb|BU990110.1|BU990110 HF24B24r HF Hordeum vulgare subsp. v... 133 2e-035
gb|AJ462971.1|AJ462971 AJ462971 S00002 Hordeum vulgare subs... 68 9e-029
gb|AV936595.1|AV936595 AV936595 K. Sato unpublished cDNA li... 105 5e-022
gb|BJ475678.1|BJ475678 BJ475678 K. Sato unpublished cDNA li... 105 5e-022
gb|BJ476594.1|BJ476594 BJ476594 K. Sato unpublished cDNA li... 105 5e-022
gb|BJ477067.1|BJ477067 BJ477067 K. Sato unpublished cDNA li... 105 5e-022
>gb|BE602597.3|BE602597 HVSMEh0100E21f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0100E21f, mRNA sequence
Length = 732
Score = 296 bits (641), Expect(2) = 2e-081
Identities = 123/179 (68%), Positives = 146/179 (81%)
Frame = +2 / +1
Query: 71 TGKMMPLSQTDFSPSQFTSSQNAAADSTTPSKMRGASSTMPLTVKQVVDAQQSGTGDKGA 250
T MM SQ D ++ AA +TP+K RGAS TMPLTVKQ+ DAQ +GTG+KG
Sbjct: 55 TKAMMFSSQVDAFSPSQFTASQNAAADSTPNKFRGASGTMPLTVKQIADAQLAGTGEKGG 234
Query: 251 PFIVNGVEMANIRLVGMVNAKVERTTDVTFTLDDGTGRLDFIRWVNDASDSFETAAIQNG 430
PF+V+GVE AN+RLVGMV+ KVER TDV+FTLDDGTGR+DFIRWVNDA+DS ETAAIQNG
Sbjct: 235 PFVVDGVETANVRLVGMVSGKVERNTDVSFTLDDGTGRIDFIRWVNDAADSAETAAIQNG 414
Query: 431 MYIAVIGSLKGLQERKRATAFSIRPITDFNEVTLHFIQCVRMHIENIELKAGSPARISS 607
MY+AVIGSLKG+Q++KRATAF++RPITD+NEVTLHFIQCVRMH EN + K GSP +S
Sbjct: 415 MYVAVIGSLKGVQDKKRATAFAVRPITDYNEVTLHFIQCVRMHAENTKSKVGSPTHTNS 591
Score = 208 bits (449), Expect(2) = 1e-057
Identities = 100/157 (63%), Positives = 108/157 (68%)
Frame = -3 / -2
Query: 609 EELIRAGLPAFNSMFSICIRTH*MKCSVTSLKSVIGLIEKAVARFLSCSPLRLPMTAMYI 430
+EL+ GLP F+ +FS CIRTH*MKC VTSL SVIGL KAVARFLS +PLRLPMTA YI
Sbjct: 593 KELVCVGLPTFDFVFSACIRTH*MKCRVTSL*SVIGLTAKAVARFLS*TPLRLPMTATYI 414
Query: 429 PF*IAAVSKESEASFTHLMXXXXXXXXXXXXXXXXXXXTLALTIPTSRMLAISTPLTMNG 250
PF*IAAVS ES ASFTHLM TL LTIPTS LA+STP T G
Sbjct: 413 PF*IAAVSAESAASFTHLMKSMRPVPSSRVKDTSVFRSTLPLTIPTSLTLAVSTPSTTKG 234
Query: 249 APLSPVPDCCASTTCFTVSGMVLDAPRILEGVVESAA 139
P SPVP CAS CFTVSG+V DAPR L GV +AA
Sbjct: 233 PPFSPVPASCASAICFTVSGIVPDAPRNLLGVESAAA 123
Score = 174 bits (375), Expect = 8e-043
Identities = 74/112 (66%), Positives = 89/112 (79%)
Frame = -2 / -1
Query: 589 TASL*FNVLYMHPNTLNEMQRNLIEIGYRPD*ESSSTLPLLQSLEASNDRNVHTILNSSS 410
TA+L*F VL MHP TLNEMQ +L+ I YR D +SSSTL +L SLEA ND N+HT+LNSSS
Sbjct: 573 TANL*FCVLCMHPYTLNEMQGDLVVISYRSDCKSSSTLLVLNSLEAPNDGNIHTVLNSSS 394
Query: 409 FKRI*SIIHPSDEIEAAGAIVEREGHIGRPLHLGIDHPHKSNVSHLDAIDDE 254
F R+ SIIHPSDE++AAGA+VE EGH+ PLHL HPH+ +V LDA+DDE
Sbjct: 393 FSRVCSIIHPSDEVDAAGAVVEGEGHVRVPLHLAAHHPHEPDVGRLDAVDDE 238
Score = 163 bits (350), Expect(3) = 4e-048
Identities = 63/94 (67%), Positives = 72/94 (76%)
Frame = +1 / +3
Query: 337 LHARRWHRPPRFHQMGE*CFRFF*NCCYSEWYVHCGHWKPQGTAREEACYCFLNQAYNRF 516
LH RR HRP R HQMGE*C R *NCCYSE YV C HW+PQG++ +EACYCF +Q YN
Sbjct: 321 LHPRRRHRPHRLHQMGE*CCRLC*NCCYSERYVCCRHWEPQGSSGQEACYCFCSQTYN*L 500
Query: 517 Q*GYAAFHSVCSDAYREH*IKGWQSCTNQFFYGS 618
Q G+ AFHSVC+DA REH IKGWQS +QFF G+
Sbjct: 501 QRGHPAFHSVCTDACREHKIKGWQSHAHQFFMGT 602
Score = 123 bits (263), Expect = 2e-027
Identities = 60/88 (68%), Positives = 69/88 (78%)
Frame = -1 / -3
Query: 617 LP*KN*FVQDCQPLIQCSLYASEHTE*NAA*PH*NRL*A*LRKQ*HASSLAVP*GFQ*PQ 438
+P KN DCQPLI CSL+AS HTE*NA *P N+L* *L+KQ*HAS +P*G Q* Q
Sbjct: 601 VPIKNWCAWDCQPLILCSLHASVHTE*NAG*PRCNQL*V*LQKQ*HASCPELP*GSQ*RQ 422
Query: 437 CTYHSE*QQFQKNLKHHSPI**NRGGRC 354
TY SE*QQFQ++L+HHSPI**+R GRC
Sbjct: 421 HTYRSE*QQFQQSLQHHSPI**SRCGRC 338
Score = 70.3 bits (147), Expect(3) = 7e-021
Identities = 33/57 (57%), Positives = 34/57 (59%)
Frame = +3 / +2
Query: 294 WGWSMPRWSGRPM*PSRSTMAPAASISSDG*MMXXXXXXXXXFRMVCTLRSLEASRD 464
WGW RWSG PS ST APAAS SSDG*MM FR VC L SL ASR+
Sbjct: 278 WGW*AARWSGTRTCPSPSTTAPAASTSSDG*MMLQTLLKLLLFRTVCMLPSLGASRE 448
Score = 41.4 bits (84), Expect(3) = 4e-048
Identities = 19/34 (55%), Positives = 20/34 (58%)
Frame = +2 / +1
Query: 53 AAPPQVTGKMMPLSQTDFSPSQFTSSQNAAADST 154
A P M FSPSQFT+SQNAAADST
Sbjct: 40 AEQPNTKAMMFSSQVDAFSPSQFTASQNAAADST 141
Score = 39.6 bits (80), Expect(3) = 7e-021
Identities = 18/32 (56%), Positives = 22/32 (68%)
Frame = +3 / +2
Query: 513 ISMRLRCISFSVFGCI*RTLN*RLAVLHESVL 608
I+ R CISFSV+GC+ RT N RLAV +L
Sbjct: 497 ITTRSPCISFSVYGCMQRTQNQRLAVPRTPIL 592
Score = 35.9 bits (72), Expect(2) = 1e-057
Identities = 14/18 (77%), Positives = 16/18 (88%)
Frame = -3 / -2
Query: 153 VESAAAFWEEVNCDGEKS 100
VESAAAF + VNCDGEK+
Sbjct: 140 VESAAAFCDAVNCDGEKA 87
Score = 33.6 bits (67), Expect(3) = 7e-021
Identities = 18/45 (40%), Positives = 23/45 (51%)
Frame = +1 / +3
Query: 19 RSSHLRTRKPIRRTSSGDREDDAVEPNRLLAVAVHLLPECRRRLH 153
R +R+R+ + D RLLAVAVH + E RRRLH
Sbjct: 6 RPPEVRSRRVPSGAAEYQSHDVLEPGRRLLAVAVHRVAERRRRLH 140
Score = 27.2 bits (53), Expect(2) = 2e-081
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1 / +2
Query: 610 YGSVILKWIQ*IKHTDIFEIQSSTGDQR 693
YG+ IL+ I+ H+ + E+Q T QR
Sbjct: 593 YGNPILQQIERSSHSTLCEVQPGTSGQR 676
Score = 26.3 bits (51), Expect(3) = 4e-048
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +1 / +3
Query: 232 HGRQGRSVHRQWRRDG 279
H R+GR++ R+ RRDG
Sbjct: 216 HRREGRALRRRRRRDG 263
>gb|AL507344.1|AL507344 AL507344 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY05B07V 5',
mRNA sequence
Length = 659
Score = 230 bits (496), Expect(2) = 4e-063
Identities = 97/142 (68%), Positives = 116/142 (81%)
Frame = +2 / +1
Query: 71 TGKMMPLSQTDFSPSQFTSSQNAAADSTTPSKMRGASSTMPLTVKQVVDAQQSGTGDKGA 250
T MM SQ D ++ AA +TP+K RGAS TMPLTVKQ+ DAQ +GTG+KG
Sbjct: 64 TKAMMFSSQVDAFSPSQFTASQNAAADSTPNKFRGASGTMPLTVKQIADAQLAGTGEKGG 243
Query: 251 PFIVNGVEMANIRLVGMVNAKVERTTDVTFTLDDGTGRLDFIRWVNDASDSFETAAIQNG 430
PF+V+GVE AN+RLVGMV+ KVER TDV+FTLDDGTGR+DFIRWVNDA+DS ETAAIQNG
Sbjct: 244 PFVVDGVETANVRLVGMVSGKVERNTDVSFTLDDGTGRIDFIRWVNDAADSAETAAIQNG 423
Query: 431 MYIAVIGSLKGLQERKRATAFS 496
MY+AVIGSLKG+Q++KRATAF+
Sbjct: 424 MYVAVIGSLKGVQDKKRATAFA 489
Score = 151 bits (325), Expect(3) = 1e-047
Identities = 74/120 (61%), Positives = 79/120 (65%)
Frame = -3 / -1
Query: 498 IEKAVARFLSCSPLRLPMTAMYIPF*IAAVSKESEASFTHLMXXXXXXXXXXXXXXXXXX 319
+ KAVARFLS +PLRLPMTA YIPF*IAAVS ES ASFTHLM
Sbjct: 491 LAKAVARFLS*TPLRLPMTATYIPF*IAAVSAESAASFTHLMKSMRPVPSSRVKDTSVFR 312
Query: 318 XTLALTIPTSRMLAISTPLTMNGAPLSPVPDCCASTTCFTVSGMVLDAPRILEGVVESAA 139
TL LTIPTS LA+STP T G P SPVP CAS CFTVSG+V DAPR L GV +AA
Sbjct: 311 STLPLTIPTSLTLAVSTPSTTKGPPFSPVPASCASAICFTVSGIVPDAPRNLLGVESAAA 132
Score = 132 bits (282), Expect(2) = 2e-036
Identities = 53/80 (66%), Positives = 65/80 (81%)
Frame = -2 / -3
Query: 493 ESSSTLPLLQSLEASNDRNVHTILNSSSFKRI*SIIHPSDEIEAAGAIVEREGHIGRPLH 314
+SSSTL +L SLEA ND N+HT+LNSSSF R+ SIIHPSDE++AAGA+VE EGH+ PLH
Sbjct: 486 KSSSTLLVLNSLEAPNDGNIHTVLNSSSFSRVCSIIHPSDEVDAAGAVVEGEGHVRVPLH 307
Query: 313 LGIDHPHKSNVSHLDAIDDE 254
L HPH+ +V LDA+DDE
Sbjct: 306 LAAHHPHEPDVGRLDAVDDE 247
Score = 100 bits (214), Expect(2) = 1e-023
Identities = 37/52 (71%), Positives = 41/52 (78%)
Frame = +1 / +3
Query: 337 LHARRWHRPPRFHQMGE*CFRFF*NCCYSEWYVHCGHWKPQGTAREEACYCF 492
LH RR HRP R HQMGE*C R *NCCYSE YV C HW+PQG++ +EACYCF
Sbjct: 330 LHPRRRHRPHRLHQMGE*CCRLC*NCCYSERYVCCRHWEPQGSSGQEACYCF 485
Score = 70.3 bits (147), Expect(4) = 6e-034
Identities = 33/57 (57%), Positives = 34/57 (59%)
Frame = +3 / +2
Query: 294 WGWSMPRWSGRPM*PSRSTMAPAASISSDG*MMXXXXXXXXXFRMVCTLRSLEASRD 464
WGW RWSG PS ST APAAS SSDG*MM FR VC L SL ASR+
Sbjct: 287 WGW*AARWSGTRTCPSPSTTAPAASTSSDG*MMLQTLLKLLLFRTVCMLPSLGASRE 457
Score = 62.5 bits (130), Expect(4) = 6e-034
Identities = 24/45 (53%), Positives = 31/45 (68%)
Frame = +2 / +2
Query: 446 IGSLKGLQERKRATAFSIRPITDFNEVTLHFIQCVRMHIENIELK 580
+G+ + + R +RPITD+NEVTLHFIQCVRMH EN + K
Sbjct: 440 LGASREFRTRSVLLLLQVRPITDYNEVTLHFIQCVRMHAENTKSK 574
Score = 41.8 bits (85), Expect(3) = 1e-047
Identities = 21/30 (70%), Positives = 23/30 (76%)
Frame = -1 / -1
Query: 578 LIQCSLYASEHTE*NAA*PH*NRL*A*LRK 489
LI CSL+AS HTE*NA *P N+L* *L K
Sbjct: 572 LILCSLHASVHTE*NAG*PRCNQL*V*LAK 483
Score = 41.4 bits (84), Expect(2) = 2e-036
Identities = 18/29 (62%), Positives = 21/29 (72%)
Frame = -2 / -2
Query: 583 SL*FNVLYMHPNTLNEMQRNLIEIGYRPD 497
+ *F VL MHP TLNEMQ +L+ I YR D
Sbjct: 577 TF*FCVLCMHPYTLNEMQGDLVVISYRSD 491
Score = 41.4 bits (84), Expect(4) = 6e-034
Identities = 19/34 (55%), Positives = 20/34 (58%)
Frame = +2 / +1
Query: 53 AAPPQVTGKMMPLSQTDFSPSQFTSSQNAAADST 154
A P M FSPSQFT+SQNAAADST
Sbjct: 49 AEQPNTKAMMFSSQVDAFSPSQFTASQNAAADST 150
Score = 35.9 bits (72), Expect(3) = 1e-047
Identities = 14/18 (77%), Positives = 16/18 (88%)
Frame = -3 / -1
Query: 153 VESAAAFWEEVNCDGEKS 100
VESAAAF + VNCDGEK+
Sbjct: 149 VESAAAFCDAVNCDGEKA 96
Score = 32.7 bits (65), Expect(2) = 4e-063
Identities = 13/21 (61%), Positives = 16/21 (76%)
Frame = +3 / +3
Query: 513 ISMRLRCISFSVFGCI*RTLN 575
I+ R CISFSV+GC+ RT N
Sbjct: 507 ITTRSPCISFSVYGCMQRTQN 569
Score = 32.2 bits (64), Expect(2) = 1e-023
Identities = 14/18 (77%), Positives = 15/18 (83%)
Frame = +1 / +3
Query: 100 RLLAVAVHLLPECRRRLH 153
RLLAVAVH + E RRRLH
Sbjct: 96 RLLAVAVHRVAERRRRLH 149
Score = 26.3 bits (51), Expect(4) = 6e-034
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +1 / +3
Query: 232 HGRQGRSVHRQWRRDG 279
H R+GR++ R+ RRDG
Sbjct: 225 HRREGRALRRRRRRDG 272
>gb|BI776636.2|BI776636 EBpi07_SQ001_D15_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ001_D15 5', mRNA sequence
Length = 526
Score = 160 bits (344), Expect(3) = 5e-047
Identities = 74/103 (71%), Positives = 79/103 (76%)
Frame = -3 / -3
Query: 693 PLVTGAGLDFKDVGVLDSLNPFENDTPIEELIRAGLPAFNSMFSICIRTH*MKCSVTSLK 514
PL GAGLDF + GV S N EN PIEEL+ GLP F+ +FS CIRTH*MKC VTSL
Sbjct: 311 PLAAGAGLDFTEGGVAASFNLLENGVPIEELVCVGLPTFDFVFSACIRTH*MKCRVTSL* 132
Query: 513 SVIGLIEKAVARFLSCSPLRLPMTAMYIPF*IAAVSKESEASF 385
SVIGL KAVARFLS +PLRLPMTA YIPF*IAAVS ES ASF
Sbjct: 131 SVIGLTAKAVARFLS*TPLRLPMTATYIPF*IAAVSAESAASF 3
Score = 160 bits (343), Expect(3) = 2e-062
Identities = 66/108 (61%), Positives = 80/108 (74%)
Frame = +2 / +1
Query: 386 NDASDSFETAAIQNGMYIAVIGSLKGLQERKRATAFSIRPITDFNEVTLHFIQCVRMHIE 565
NDA+DS ETAAIQNGMY+AVIGSLKG+Q++KRATAF++RPITD+NEVTLHFIQCVRMH E
Sbjct: 4 NDAADSAETAAIQNGMYVAVIGSLKGVQDKKRATAFAVRPITDYNEVTLHFIQCVRMHAE 183
Query: 566 NIELKAGSPARISSSMGVSFSNGFXXXXXXXXXXXXXAPVTSGSSDTD 709
N + K GSP +SSMG FSN AP SG++ ++
Sbjct: 184 NTKSKVGSPTHTNSSMGTPFSNRLNEAATPPSVKSNPAPAASGTNGSE 327
Score = 155 bits (332), Expect(2) = 4e-041
Identities = 61/103 (59%), Positives = 75/103 (72%)
Frame = +1 / +3
Query: 385 E*CFRFF*NCCYSEWYVHCGHWKPQGTAREEACYCFLNQAYNRFQ*GYAAFHSVCSDAYR 564
E*C R *NCCYSE YV C HW+PQG++ +EACYCF +Q YN Q G+ AFHSVC+DA R
Sbjct: 3 E*CCRLC*NCCYSERYVCCRHWEPQGSSGQEACYCFCSQTYN*LQRGHPAFHSVCTDACR 182
Query: 565 EH*IKGWQSCTNQFFYGSVILKWIQ*IKHTDIFEIQSSTGDQR 693
EH IKGWQS +QFFYG+ IL+ I+ H+ + E+Q T QR
Sbjct: 183 EHKIKGWQSHAHQFFYGNPILQQIERSSHSTLCEVQPGTSGQR 311
Score = 122 bits (260), Expect(2) = 1e-028
Identities = 62/103 (60%), Positives = 71/103 (68%)
Frame = -1 / -1
Query: 692 RWSPVLDWISKMSVCLIH*IHLRMTLP*KN*FVQDCQPLIQCSLYASEHTE*NAA*PH*N 513
RW V W S+ L+ I RM P*KN DCQPLI CSL+AS HTE*NA *P N
Sbjct: 310 RWPLVPGWTSQRVEWLLRSICWRMGFP*KNWCAWDCQPLILCSLHASVHTE*NAG*PRCN 131
Query: 512 RL*A*LRKQ*HASSLAVP*GFQ*PQCTYHSE*QQFQKNLKHHS 384
+L* *L+KQ*HAS +P*G Q* Q TY SE*QQFQ++L+HHS
Sbjct: 130 QL*V*LQKQ*HASCPELP*GSQ*RQHTYRSE*QQFQQSLQHHS 2
Score = 116 bits (248), Expect = 3e-025
Identities = 58/102 (56%), Positives = 68/102 (66%)
Frame = -2 / -2
Query: 691 AGHRCWTGFQRCRCA*FTESI*E*HSHRRTDSCRTASL*FNVLYMHPNTLNEMQRNLIEI 512
AG C G R F +S+ E SHRR TA+L*F VL MHP TLNEMQ +L+ I
Sbjct: 309 AGRWCRVGLHRGWSGCFVQSVGEWGSHRRIGVRGTANL*FCVLCMHPYTLNEMQGDLVVI 130
Query: 511 GYRPD*ESSSTLPLLQSLEASNDRNVHTILNSSSFKRI*SII 386
YR D +SSSTL +L SLEA ND N+HT+LNSSSF R+ SII
Sbjct: 129 SYRSDCKSSSTLLVLNSLEAPNDGNIHTVLNSSSFSRVCSII 4
Score = 68.4 bits (143), Expect(3) = 2e-062
Identities = 24/39 (61%), Positives = 33/39 (84%)
Frame = +2 / +1
Query: 689 SGSSDTDLHTQVLNFFNEPANLESEHGVHVDEVLKRFKL 805
+ S+TD +TQVLN F EPAN+ES+HGVH+D++ KRF+L
Sbjct: 313 TNGSETDFYTQVLNVFREPANMESDHGVHMDDIAKRFRL 429
Score = 50.6 bits (104), Expect(3) = 2e-062
Identities = 18/30 (60%), Positives = 25/30 (83%)
Frame = +2 / +1
Query: 806 LPKKQITDAIDYNMDSGRLYSTIDEFHYKA 895
LP +I +AIDY++D G +YSTID+FHYK+
Sbjct: 427 LPALKIKEAIDYHVDVGHIYSTIDDFHYKS 516
Score = 35.9 bits (72), Expect(3) = 5e-047
Identities = 20/44 (45%), Positives = 22/44 (50%)
Frame = -3 / -3
Query: 804 SLNRXXXXXXXXXXXXSRFAGSLKKFRTCVCRSVSDDPLVTGAG 673
SLNR S FAGS K F TCV +SVSD + AG
Sbjct: 428 SLNRLAMSSICTPWSLSMFAGSRKTFSTCV*KSVSDPFVPLAAG 297
Score = 34.1 bits (68), Expect(2) = 4e-041
Identities = 17/63 (26%), Positives = 34/63 (53%)
Frame = +3 / +2
Query: 708 ICTRRS*IFLMNQRTSRVSMGCTLMKYSSGSNFCRRSRSRMLLITIWTRGVFTQQLMNST 887
I T R *+ +N++T RV+M C M SGS++ R+ T+ + ++++T
Sbjct: 332 ISTHRC*MSSVNRQTWRVTMACIWMTLPSGSDYQHSRSRRLSTTTLMSGTSTRPSMISTT 511
Query: 888 TRQ 896
+++
Sbjct: 512 SQR 520
Score = 30.9 bits (61), Expect(3) = 5e-047
Identities = 17/28 (60%), Positives = 19/28 (67%)
Frame = -3 / -3
Query: 891 L*WNSSIVE*RRPESIL*SIASVICFFG 808
L*W SS+VE* P S *SIAS+I G
Sbjct: 512 L*WKSSMVE*MCPTSTW*SIASLILSAG 429
Score = 25.4 bits (49), Expect(2) = 1e-028
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = -1 / -1
Query: 782 HQRAPHAHSRGSLVH*KNSGPACADQ 705
H A H HS VH ++S P C ++
Sbjct: 406 HPYARHGHSPCLPVHGRHSAPVCRNR 329
>gb|BQ764915.1|BQ764915 EBca01_SQ005_B12_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ005_B12 5', mRNA sequence
Length = 657
Score = 133 bits (285), Expect(2) = 2e-035
Identities = 49/116 (42%), Positives = 78/116 (67%)
Frame = +2 / +2
Query: 233 TGDKGAPFIVNGVEMANIRLVGMVNAKVERTTDVTFTLDDGTGRLDFIRWVNDASDSFET 412
T D + F VNG E++ +RLVG + K+ER TDV F LDDGTGR++ RW N+ SD+ E
Sbjct: 248 TNDDKSSFAVNGTEVSTVRLVGRMMNKIERVTDVQFVLDDGTGRIEVNRWANETSDTTEM 427
Query: 413 AAIQNGMYIAVIGSLKGLQERKRATAFSIRPITDFNEVTLHFIQCVRMHIENIELK 580
+++G Y+ V G LK Q ++ A+S+R +T++N++T HF+ C+ +H++ + K
Sbjct: 428 GLVKDGDYVIVNGGLKSFQGKRHIVAYSVRLVTNYNDITHHFLYCIYVHLDLAKAK 595
Score = 36.8 bits (74), Expect(2) = 2e-035
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +2 / +2
Query: 161 SKMRGASSTMPLTVKQVVDAQQS 229
SK RGA + +PLTVKQ++DA Q+
Sbjct: 182 SKSRGAQTLLPLTVKQIMDATQT 250
>gb|BU990110.1|BU990110 HF24B24r HF Hordeum vulgare subsp. vulgare cDNA clone HF24B24
5-PRIME, mRNA sequence
Length = 593
Score = 133 bits (285), Expect(2) = 2e-035
Identities = 49/116 (42%), Positives = 78/116 (67%)
Frame = +2 / +2
Query: 233 TGDKGAPFIVNGVEMANIRLVGMVNAKVERTTDVTFTLDDGTGRLDFIRWVNDASDSFET 412
T D + F VNG E++ +RLVG + K+ER TDV F LDDGTGR++ RW N+ SD+ E
Sbjct: 146 TNDDKSSFAVNGTEVSTVRLVGRMMNKIERVTDVQFVLDDGTGRIEVNRWANETSDTTEM 325
Query: 413 AAIQNGMYIAVIGSLKGLQERKRATAFSIRPITDFNEVTLHFIQCVRMHIENIELK 580
+++G Y+ V G LK Q ++ A+S+R +T++N++T HF+ C+ +H++ + K
Sbjct: 326 GLVKDGDYVIVNGGLKSFQGKRHIVAYSVRLVTNYNDITHHFLYCIYVHLDLAKAK 493
Score = 36.8 bits (74), Expect(2) = 2e-035
Identities = 14/23 (60%), Positives = 19/23 (82%)
Frame = +2 / +2
Query: 161 SKMRGASSTMPLTVKQVVDAQQS 229
SK RGA + +PLTVKQ++DA Q+
Sbjct: 80 SKSRGAQTLLPLTVKQIMDATQT 148
>gb|AJ462971.1|AJ462971 AJ462971 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200043C12F1, mRNA sequence
Length = 420
Score = 68.4 bits (143), Expect(3) = 9e-029
Identities = 24/39 (61%), Positives = 33/39 (84%)
Frame = +2 / +3
Query: 689 SGSSDTDLHTQVLNFFNEPANLESEHGVHVDEVLKRFKL 805
+ S+TD +TQVLN F EPAN+ES+HGVH+D++ KRF+L
Sbjct: 135 TNGSETDFYTQVLNVFREPANMESDHGVHMDDIAKRFRL 251
Score = 50.6 bits (104), Expect(3) = 9e-029
Identities = 18/30 (60%), Positives = 25/30 (83%)
Frame = +2 / +3
Query: 806 LPKKQITDAIDYNMDSGRLYSTIDEFHYKA 895
LP +I +AIDY++D G +YSTID+FHYK+
Sbjct: 249 LPALKIKEAIDYHVDVGHIYSTIDDFHYKS 338
Score = 46.4 bits (95), Expect(3) = 9e-029
Identities = 18/39 (46%), Positives = 26/39 (66%)
Frame = +1 / +2
Query: 577 KGWQSCTNQFFYGSVILKWIQ*IKHTDIFEIQSSTGDQR 693
+GWQS +QFFYG+ IL+ I+ H+ + E+Q T QR
Sbjct: 17 QGWQSHAHQFFYGNPILQQIERSSHSTLCEVQPGTSGQR 133
>gb|AV936595.1|AV936595 AV936595 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal5b09 3', mRNA sequence
Length = 687
Score = 105 bits (224), Expect = 5e-022
Identities = 40/105 (38%), Positives = 65/105 (61%)
Frame = +2 / -1
Query: 251 PFIVNGVEMANIRLVGMVNAKVERTTDVTFTLDDGTGRLDFIRWVNDASDSFETAAIQNG 430
PF+++G+ ++++ L+GMV K TF L DGTG ++ I W A DS + + NG
Sbjct: 582 PFVIDGMAVSSVELLGMVTNKTVSMDHCTFDLYDGTGAVNVIYWFEGAQDSKDAWSFSNG 403
Query: 431 MYIAVIGSLKGLQERKRATAFSIRPITDFNEVTLHFIQCVRMHIE 565
MY+ V+G +++ + A+ +RPI +FN+VT HFI C+ HI+
Sbjct: 402 MYVRVVGRTTSMEQFFQIKAYIVRPINNFNDVTHHFIYCIYAHID 268
>gb|BJ475678.1|BJ475678 BJ475678 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal29h02 3', mRNA sequence
Length = 662
Score = 105 bits (224), Expect = 5e-022
Identities = 40/105 (38%), Positives = 65/105 (61%)
Frame = +2 / -3
Query: 251 PFIVNGVEMANIRLVGMVNAKVERTTDVTFTLDDGTGRLDFIRWVNDASDSFETAAIQNG 430
PF+++G+ ++++ L+GMV K TF L DGTG ++ I W A DS + + NG
Sbjct: 582 PFVIDGMAVSSVELLGMVTNKTVSMDHCTFDLYDGTGAVNVIYWFEGAQDSKDAWSFSNG 403
Query: 431 MYIAVIGSLKGLQERKRATAFSIRPITDFNEVTLHFIQCVRMHIE 565
MY+ V+G +++ + A+ +RPI +FN+VT HFI C+ HI+
Sbjct: 402 MYVRVVGRTTSMEQFFQIKAYIVRPINNFNDVTHHFIYCIYAHID 268
>gb|BJ476594.1|BJ476594 BJ476594 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal35n21 3', mRNA sequence
Length = 626
Score = 105 bits (224), Expect = 5e-022
Identities = 40/105 (38%), Positives = 65/105 (61%)
Frame = +2 / -1
Query: 251 PFIVNGVEMANIRLVGMVNAKVERTTDVTFTLDDGTGRLDFIRWVNDASDSFETAAIQNG 430
PF+++G+ ++++ L+GMV K TF L DGTG ++ I W A DS + + NG
Sbjct: 581 PFVIDGMAVSSVELLGMVTNKTVSMDHCTFDLYDGTGAVNVIYWFEGAQDSKDAWSFSNG 402
Query: 431 MYIAVIGSLKGLQERKRATAFSIRPITDFNEVTLHFIQCVRMHIE 565
MY+ V+G +++ + A+ +RPI +FN+VT HFI C+ HI+
Sbjct: 401 MYVRVVGRTTSMEQFFQIKAYIVRPINNFNDVTHHFIYCIYAHID 267
>gb|BJ477067.1|BJ477067 BJ477067 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal39k22 3', mRNA sequence
Length = 717
Score = 105 bits (224), Expect = 5e-022
Identities = 40/105 (38%), Positives = 65/105 (61%)
Frame = +2 / -1
Query: 251 PFIVNGVEMANIRLVGMVNAKVERTTDVTFTLDDGTGRLDFIRWVNDASDSFETAAIQNG 430
PF+++G+ ++++ L+GMV K TF L DGTG ++ I W A DS + + NG
Sbjct: 606 PFVIDGMAVSSVELLGMVTNKTVSMDHCTFDLYDGTGAVNVIYWFEGAQDSKDAWSFSNG 427
Query: 431 MYIAVIGSLKGLQERKRATAFSIRPITDFNEVTLHFIQCVRMHIE 565
MY+ V+G +++ + A+ +RPI +FN+VT HFI C+ HI+
Sbjct: 426 MYVRVVGRTTSMEQFFQIKAYIVRPINNFNDVTHHFIYCIYAHID 292
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 520,999,229
Number of Sequences: 312970
Number of extensions: 7509388
Number of successful extensions: 43147
Number of sequences better than 1.0e-020: 13
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 15504375299
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)