TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161272.2.1
(747 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CB881182.1|CB881182 HM09A07w HM Hordeum vulgare subsp. v... 205 2e-052
gb|CK122346.1|CK122346 BES1824102p12 BES1824 Hordeum vulgar... 109 9e-052
gb|CB881625.1|CB881625 HM10G06w HM Hordeum vulgare subsp. v... 116 1e-050
gb|AV917139.1|AV917139 AV917139 K. Sato unpublished cDNA li... 105 1e-050
gb|BE060246.3|BE060246 HVSMEg0011L08f Hordeum vulgare pre-a... 101 2e-049
gb|BF622288.3|BF622288 HVSMEa0002I16f Hordeum vulgare seedl... 98 1e-048
gb|BQ759881.1|BQ759881 EBpi05_SQ004_P16_R pistil, 8 DPA, no... 134 1e-048
gb|CB880524.1|CB880524 HM06N05w HM Hordeum vulgare subsp. v... 116 2e-047
gb|BF254126.2|BF254126 HVSMEf0003B13f Hordeum vulgare seedl... 105 2e-045
gb|CK123980.1|CK123980 BES1824106f16 BES1824 Hordeum vulgar... 105 4e-044
gb|BF260026.3|BF260026 HVSMEf0020O13f Hordeum vulgare seedl... 134 6e-044
gb|BQ661443.1|BQ661443 HM03I08u HM Hordeum vulgare subsp. v... 116 1e-043
gb|BM442635.2|BM442635 EBan01_SQ003_L19_R anther, yellow st... 173 8e-043
gb|BI956772.1|BI956772 HVSMEn0005E06f Hordeum vulgare rachi... 105 1e-042
gb|CV058074.1|CV058074 BNEL34a7 Barley EST endosperm librar... 91 3e-041
gb|CK122737.1|CK122737 BES1824105j22 BES1824 Hordeum vulgar... 105 5e-041
gb|BE454367.2|BE454367 HVSMEh0093N18f Hordeum vulgare 5-45 ... 106 2e-040
gb|BQ468475.1|BQ468475 HM01F21T HM Hordeum vulgare subsp. v... 161 4e-039
gb|BF628930.2|BF628930 HVSMEb0009C23f Hordeum vulgare seedl... 103 6e-039
gb|BM097618.2|BM097618 EBem04_SQ002_D15_R embryo, 12 DPA, n... 100 7e-039
gb|BM442090.2|BM442090 EBan01_SQ002_C16_R anther, yellow st... 159 2e-038
gb|BI959138.1|BI959138 HVSMEn0018F10f Hordeum vulgare rachi... 105 2e-038
gb|BQ753409.1|BQ753409 EBan01_SQ004_D03_R anther, yellow st... 159 2e-038
gb|BF253787.2|BF253787 HVSMEf0002C01f Hordeum vulgare seedl... 103 6e-038
gb|BQ465138.1|BQ465138 HU02M06r HU Hordeum vulgare subsp. v... 105 2e-037
gb|CB883976.1|CB883976 HM06N05r HM Hordeum vulgare subsp. v... 155 2e-037
gb|BI950777.1|BI950777 HVSMEl0023C15f Hordeum vulgare spike... 106 4e-037
gb|AV928430.1|AV928430 AV928430 K. Sato unpublished cDNA li... 134 7e-037
gb|CV055940.1|CV055940 BNEL12B5 Barley EST endosperm librar... 95 1e-036
gb|BQ459628.1|BQ459628 HA08J01r HA Hordeum vulgare subsp. v... 89 7e-036
gb|BE196421.3|BE196421 HVSMEh0092D24f Hordeum vulgare 5-45 ... 105 4e-034
gb|BU980984.1|BU980984 HA22E18r HA Hordeum vulgare subsp. v... 80 5e-033
gb|BM098205.2|BM098205 EBpi03_SQ002_N14_R pistil, 4 DPA, no... 80 5e-033
gb|BJ463021.1|BJ463021 BJ463021 K. Sato unpublished cDNA li... 93 7e-033
gb|CV054003.1|CV054003 BNEL105f8 Barley EST endosperm libra... 99 8e-033
gb|CV063217.1|CV063217 BNEL87h3 Barley EST endosperm librar... 99 5e-032
gb|BQ753555.1|BQ753555 EBan01_SQ004_J12_R anther, yellow st... 137 9e-032
gb|BQ459308.1|BQ459308 HA01I15r HA Hordeum vulgare subsp. v... 87 3e-031
gb|CA019997.1|CA019997 HV14A15r HV Hordeum vulgare subsp. v... 105 5e-030
gb|BI958784.1|BI958784 HVSMEn0016J13f Hordeum vulgare rachi... 105 7e-030
gb|BQ469137.1|BQ469137 HM03I08r HM Hordeum vulgare subsp. v... 130 7e-030
gb|BU995044.1|BU995044 HM09A07r HM Hordeum vulgare subsp. v... 104 1e-029
gb|BI957603.1|BI957603 HVSMEn0010F11f Hordeum vulgare rachi... 105 2e-029
gb|BE230894.2|BE230894 HVSMEg0001I02f Hordeum vulgare pre-a... 127 5e-029
gb|BQ467895.1|BQ467895 HR01E04r HR Hordeum vulgare subsp. v... 127 9e-029
gb|BI959353.1|BI959353 HVSMEn0019F12f Hordeum vulgare rachi... 105 2e-028
gb|CB880652.1|CB880652 HM07E17w HM Hordeum vulgare subsp. v... 126 2e-028
gb|CB881234.1|CB881234 HM09C23w HM Hordeum vulgare subsp. v... 126 2e-028
gb|AV917082.1|AV917082 AV917082 K. Sato unpublished cDNA li... 100 2e-028
gb|CV063939.1|CV063939 BNEL94h5 Barley EST endosperm librar... 99 3e-028
gb|BU981055.1|BU981055 HA22I03r HA Hordeum vulgare subsp. v... 99 3e-028
gb|BQ458429.1|BQ458429 HA05E07r HA Hordeum vulgare subsp. v... 99 3e-028
gb|BU967134.1|BU967134 HB03G05r BC Hordeum vulgare subsp. v... 99 3e-028
gb|BI960458.1|BI960458 HVSMEn0024K16f Hordeum vulgare rachi... 105 5e-028
gb|BF256653.2|BF256653 HVSMEf0010J19f Hordeum vulgare seedl... 105 1e-027
gb|BU995090.1|BU995090 HM09C23r HM Hordeum vulgare subsp. v... 105 1e-027
gb|BI959698.1|BI959698 HVSMEn0020N13f Hordeum vulgare rachi... 105 3e-027
gb|BQ470849.1|BQ470849 HX04D14r HX Hordeum vulgare subsp. v... 98 9e-027
gb|CK124171.1|CK124171 BES1824107e17 BES1824 Hordeum vulgar... 89 1e-026
gb|BG369116.2|BG369116 HVSMEi0021M10f Hordeum vulgare 20 DA... 67 1e-026
gb|BI960089.1|BI960089 HVSMEn0023C15f Hordeum vulgare rachi... 109 2e-026
gb|CV060611.1|CV060611 BNEL5B4 Barley EST endosperm library... 99 2e-026
gb|BI959771.1|BI959771 HVSMEn0021G03f Hordeum vulgare rachi... 105 4e-026
gb|CK125696.1|CK125696 BES1824110b08 BES1824 Hordeum vulgar... 100 2e-025
gb|CV060955.1|CV060955 BNEL63c5 Barley EST endosperm librar... 54 3e-025
gb|BG369408.2|BG369408 HVSMEi0024E17f Hordeum vulgare 20 DA... 105 4e-025
gb|CA017263.1|CA017263 HV14A15u HV Hordeum vulgare subsp. v... 87 2e-024
gb|CB883447.1|CB883447 HQ02E17w HQ Hordeum vulgare subsp. v... 100 2e-024
gb|BM101447.2|BM101447 EBpi01_SQ003_O01_R pistil, 1 DPA, no... 87 2e-024
gb|BI956858.1|BI956858 HVSMEn0005K24f Hordeum vulgare rachi... 89 3e-024
gb|BQ767787.1|BQ767787 EBro08_SQ009_H22_R root, 3 week, dro... 101 6e-024
gb|BF257093.3|BF257093 HVSMEf0011N11f Hordeum vulgare seedl... 65 9e-024
gb|CA019924.1|CA019924 HV13L18r HV Hordeum vulgare subsp. v... 65 9e-024
gb|BI957183.1|BI957183 HVSMEn0007P24f Hordeum vulgare rachi... 102 1e-023
gb|BM372935.2|BM372935 EBma04_SQ002_H04_R maternal, 10 DPA,... 109 2e-023
gb|BJ453165.1|BJ453165 BJ453165 K. Sato unpublished cDNA li... 108 3e-023
gb|BJ460714.1|BJ460714 BJ460714 K. Sato unpublished cDNA li... 108 3e-023
gb|BU986289.1|BU986289 HF11E17r HF Hordeum vulgare subsp. v... 108 4e-023
gb|BG310251.1|BG310251 HVSMEc0016K12f Hordeum vulgare seedl... 105 2e-022
gb|BU980194.1|BU980194 HA18P06r HA Hordeum vulgare subsp. v... 105 2e-022
gb|CA031744.1|CA031744 HX11A03r HX Hordeum vulgare subsp. v... 105 2e-022
gb|BI959720.1|BI959720 HVSMEn0020P05f Hordeum vulgare rachi... 105 4e-022
gb|BG365461.2|BG365461 HVSMEi0002M09f Hordeum vulgare 20 DA... 105 4e-022
gb|CA022206.1|CA022206 HZ42H09r HZ Hordeum vulgare subsp. v... 105 4e-022
gb|AL504600.1|AL504600 AL504600 Hordeum vulgare Barke roots... 81 7e-022
gb|BI957582.1|BI957582 HVSMEn0010D14f Hordeum vulgare rachi... 104 7e-022
gb|CB882163.1|CB882163 HL01B01w HL Hordeum vulgare subsp. v... 104 7e-022
gb|CK124612.1|CK124612 BES1824109m10 BES1824 Hordeum vulgar... 103 1e-021
gb|CW512145.1|CW512145 ToNAR_Morex_118b Morex Exon trapped ... 103 1e-021
gb|BF259343.2|BF259343 HVSMEf0019F11f Hordeum vulgare seedl... 103 1e-021
gb|AV923254.1|AV923254 AV923254 K. Sato unpublished cDNA li... 103 1e-021
gb|CA022237.1|CA022237 HZ42I19r HZ Hordeum vulgare subsp. v... 103 1e-021
gb|CA023986.1|CA023986 HZ48A15r HZ Hordeum vulgare subsp. v... 103 1e-021
gb|BI959654.1|BI959654 HVSMEn0020K03f Hordeum vulgare rachi... 101 4e-021
>gb|CB881182.1|CB881182 HM09A07w HM Hordeum vulgare subsp. vulgare cDNA clone HM09A07
3-PRIME, mRNA sequence
Length = 680
Score = 205 bits (443), Expect = 2e-052
Identities = 77/138 (55%), Positives = 103/138 (74%)
Frame = +3 / -3
Query: 42 IAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYPAGQKIVFHIEKGCNPNY 221
IA YHFDLSG AFG++AK G KLR G ++++FRRV+CKYP G K+ FH+EKG +PNY
Sbjct: 678 IAAYHFDLSGHAFGTMAKKGQEQKLRDAGEVEIKFRRVKCKYPEGTKVNFHVEKGSSPNY 499
Query: 222 LAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGK 401
LA++VKF+ DGD+V ++I+ K +W +K SWGA+WR+DT L GPFS+R T+E G
Sbjct: 498 LALVVKFLQGDGDVVAVDIKPKGKDKWIELKESWGAVWRIDTPDKLIGPFSVRYTTEGGT 319
Query: 402 KVIAKDVIPPNWRPDAVY 455
K +A+DVIP W+PD Y
Sbjct: 318 KTVAEDVIPEGWKPDTSY 265
Score = 116 bits (247), Expect = 2e-025
Identities = 59/135 (43%), Positives = 69/135 (51%)
Frame = -3 / +2
Query: 445 SGLQFGGMTSLAMTFLPDSLVRRMEKGPLSALAVSILQMAPQESFMGFHSADNLXXXXXX 266
SG Q GMTS A +P S+V R E GP+S VSILQ AP +S H + L
Sbjct: 275 SGFQPSGMTSSATVLVPPSVV*RTENGPMSLSGVSILQTAPHDSLSSIHLSLPLGLMSTA 454
Query: 265 XXXXXSATNFTSTAR*LGLQPFSMWNTIFCPAGYLHRTLLNSTSMMPQWRSLSLSPGFAR 86
N T+ AR*LGL+PFS W P+GYLH T N S P RS P A
Sbjct: 455 TTSPSPWRNLTTRAR*LGLEPFSTWKFTLVPSGYLHLTRRNLISTSPASRSFCSWPFLAM 634
Query: 85 EPKALPLKSKW*GAI 41
PKA P +SKW A+
Sbjct: 635 VPKAWPDRSKWYAAM 679
Score = 100 bits (213), Expect = 9e-021
Identities = 55/141 (39%), Positives = 72/141 (51%)
Frame = +2 / -2
Query: 41 YRSLPLRLERQGLRLPGKARAQRQASPLRHHGRRVQKGAVQVPRRAEDCVPHREGLQPQL 220
+R +PLR R LR G+ +A+ R G +V VQVP + +P EGLQPQL
Sbjct: 679 HRGVPLRPVRPCLRHHGQEGPGAEAARRRRGGDQVPPRQVQVPGGHQGELPRGEGLQPQL 500
Query: 221 PGRACEVCGGRR*HRADGNPGQVVG*VEAHEALLGRHLEDGHCQGAQGPLLHPPHQRVRQ 400
P C+V GRR G+ Q G V+ + ++G LED H A P+L P H R R
Sbjct: 499 PRPGCQVPPGRRRRGGRGHQAQGQGQVDRAQGVVGSRLEDRHT*QAHRPILRPLHHRGRH 320
Query: 401 EGHRQRRHPAELETRCRLHFQ 463
+ R+ RHP LE R L Q
Sbjct: 319 QDRRRGRHPRGLEARHLLRDQ 257
>gb|CK122346.1|CK122346 BES1824102p12 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P122 5-PRIME, mRNA sequence
Length = 717
Score = 109 bits (232), Expect(3) = 9e-052
Identities = 41/54 (75%), Positives = 47/54 (87%)
Frame = +3 / +1
Query: 9 TVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
TV ITDMNY P+APYHFDLSG AFG LAKPGLND+LRH GI+D+EF RV C++P
Sbjct: 115 TVIITDMNYYPVAPYHFDLSGTAFGRLAKPGLNDRLRHSGIIDIEFTRVPCEFP 276
Score = 90.0 bits (190), Expect(3) = 8e-030
Identities = 39/55 (70%), Positives = 44/55 (80%)
Frame = -3 / -2
Query: 169 GYLHRTLLNSTSMMPQWRSLSLSPGFAREPKALPLKSKW*GAIGS*FMSVMYTVT 5
G H T +NS SMMP+WRSLSLSPGFA P A+PL+SKW*GA G *FMSVM TV+
Sbjct: 275 GNSHGTRVNSMSMMPEWRSLSLSPGFASLPNAVPLRSKW*GATG**FMSVMITVS 111
Score = 88.6 bits (187), Expect(3) = 9e-052
Identities = 33/69 (47%), Positives = 47/69 (68%)
Frame = +3 / +1
Query: 267 LMEIQDKLSAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPD 446
LME + +W M+ SWG++WR+D+ L+ PFSIR+ +ESGK ++A VIP NWRP+
Sbjct: 379 LMESRGPGGGKWTRMRESWGSVWRLDSNHRLQAPFSIRIRNESGKTLVANKVIPANWRPN 558
Query: 447 AVYTSNVQF 473
Y S VQ+
Sbjct: 559 TFYRSFVQY 585
Score = 81.7 bits (172), Expect(4) = 3e-023
Identities = 36/52 (69%), Positives = 40/52 (76%)
Frame = -2 / -1
Query: 164 LAPHPSELDVHDAAVAKLVVEPGLCQGAEGLAAQVEVVGSDRLVVHVSDVHR 9
LA HP ELDV DA VA+ VVEPGLC+ AE AA+VEVVG R+VVHV D HR
Sbjct: 270 LARHPCELDVDDAGVAEPVVEPGLCEPAERCAAEVEVVGRHRVVVHVGDDHR 115
Score = 71.6 bits (150), Expect(3) = 5e-024
Identities = 32/53 (60%), Positives = 36/53 (67%)
Frame = +2 / +3
Query: 11 GVHH*HELRAYRSLPLRLERQGLRLPGKARAQRQASPLRHHGRRVQKGAVQVP 169
G HH HEL +LPLR +R +R KARAQRQA PLRHH RV GAV+VP
Sbjct: 117 GDHHRHELLPGGALPLRPQRHSVRQARKARAQRQAPPLRHHRHRVHTGAVRVP 275
Score = 48.7 bits (100), Expect(3) = 5e-024
Identities = 24/66 (36%), Positives = 34/66 (51%)
Frame = +2 / +3
Query: 299 VEAHEALLGRHLEDGHCQGAQGPLLHPPHQRVRQEGHRQRRHPAELETRCRLHFQRPILL 478
V+ E ++G L G +LHP QRVRQ+ RQ+ HP +LE + L R + L
Sbjct: 411 VDEDEGVVGLRLAPRLQPPPPGAILHPHPQRVRQDARRQQGHPGQLEAQHLLPLLRAVQL 590
Query: 479 DFEFPS 496
+PS
Sbjct: 591 KSLYPS 608
Score = 44.6 bits (91), Expect(3) = 9e-052
Identities = 17/37 (45%), Positives = 26/37 (70%)
Frame = +3 / +1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G KI FH+E+ NP Y AVLV++ DGD+V +++ +
Sbjct: 277 GLKIGFHVEEYSNPVYFAVLVEYEDGDGDVVQVDLME 387
Score = 44.1 bits (90), Expect(3) = 8e-030
Identities = 25/56 (44%), Positives = 28/56 (50%)
Frame = -3 / -2
Query: 466 TLEV*TASGLQFGGMTSLAMTFLPDSLVRRMEKGPLSALAVSILQMAPQESFMGFH 299
T E * GLQ GMT LA + LPDSL RME G S Q P +S + H
Sbjct: 578 TKER*KVLGLQLAGMTLLATSVLPDSLRMRMENGAWRRWLESRRQTEPHDSLILVH 411
Score = 34.1 bits (68), Expect(3) = 8e-030
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = -2 / -1
Query: 278 DFHQHDVTVVRHKLHKHGQVVGVAALLDVEHNLLP 174
+ H H V V LH+H +V GV LL VE +L P
Sbjct: 381 EVHLHHVAVAVLVLHQHREVDGVRVLLHVEPDLEP 277
Score = 28.1 bits (55), Expect(3) = 5e-024
Identities = 15/35 (42%), Positives = 16/35 (45%)
Frame = +1 / +2
Query: 175 GRRLCSTSRRAATPTTWPCL*SLWRTTVTSC*WKS 279
G R ST R TP+T C S T T C W S
Sbjct: 278 GSRSGSTWRSTRTPSTSRCWWSTRTATATWCRWTS 382
Score = 28.1 bits (55), Expect(4) = 3e-023
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = -2 / -1
Query: 470 LDVGSVDGIGSPVRRDDV 417
L G+V+G+G PV RDD+
Sbjct: 582 LHEGAVEGVGPPVGRDDL 529
Score = 26.7 bits (52), Expect(4) = 3e-023
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = -3 / -2
Query: 235 TSTAR*LGLQPFSMWNTIFCP 173
TSTA+* G + S WN I P
Sbjct: 338 TSTAK*TGFEYSSTWNPILSP 276
Score = 26.3 bits (51), Expect(4) = 3e-023
Identities = 11/17 (64%), Positives = 11/17 (64%)
Frame = -2 / -1
Query: 344 VHPPDGAPRELHGLPLS 294
V PDGAPR H PLS
Sbjct: 456 VEAPDGAPRLPHPRPLS 406
>gb|CB881625.1|CB881625 HM10G06w HM Hordeum vulgare subsp. vulgare cDNA clone HM10G06
3-PRIME, mRNA sequence
Length = 588
Score = 116 bits (247), Expect(2) = 1e-050
Identities = 44/59 (74%), Positives = 50/59 (84%)
Frame = +3 / -1
Query: 297 EWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQF 473
+W PM LSWGAIWR D A A+K PFSIRLTSESGKK+IA+DVIP NW+PD VY SN+QF
Sbjct: 381 KWIPMTLSWGAIWRWDGANAIKAPFSIRLTSESGKKLIAQDVIPANWKPDTVYQSNIQF 205
Score = 104 bits (221), Expect(2) = 1e-050
Identities = 41/63 (65%), Positives = 49/63 (77%)
Frame = +3 / -1
Query: 99 GLNDKLRHCGIMDVEFRRVRCKYPAGQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEI 278
G LR G ++++FRRVRCKY G K+ FH+E G NPNYLA+LVKFVADDGDIV MEI
Sbjct: 582 GNEQTLRSFGELELQFRRVRCKYAPGTKVTFHVETGSNPNYLAILVKFVADDGDIVQMEI 403
Query: 279 QDK 287
Q+K
Sbjct: 402 QEK 394
Score = 87.2 bits (184), Expect(2) = 9e-027
Identities = 36/61 (59%), Positives = 43/61 (70%)
Frame = +2 / -3
Query: 299 VEAHEALLGRHLEDGHCQGAQGPLLHPPHQRVRQEGHRQRRHPAELETRCRLHFQRPILL 478
V+ + ++GRHLE G Q QGPLLHPPHQRVRQE HR RHP +LE R RL + P+L
Sbjct: 379 VDPDDLVVGRHLEVGRRQCDQGPLLHPPHQRVRQEAHRPGRHPRQLEARHRLPVKYPVLE 200
Query: 479 D 481
D
Sbjct: 199 D 197
Score = 87.2 bits (184), Expect(2) = 2e-028
Identities = 39/59 (66%), Positives = 43/59 (72%)
Frame = -3 / +2
Query: 475 *NWTLEV*TASGLQFGGMTSLAMTFLPDSLVRRMEKGPLSALAVSILQMAPQESFMGFH 299
*NW + *T SG Q GMTS AM+FLPDSLVRRMEKG L ALA S LQMAP + +G H
Sbjct: 203 *NWIFDW*TVSGFQLAGMTSWAMSFLPDSLVRRMEKGALIALAPSHLQMAPHDKVIGIH 379
Score = 58.8 bits (122), Expect(2) = 2e-028
Identities = 30/66 (45%), Positives = 41/66 (62%)
Frame = -2 / +1
Query: 293 RQLVLDFHQHDVTVVRHKLHKHGQVVGVAALLDVEHNLLPGGVLAPHPSELDVHDAAVAK 114
R+ +L+ H HDV VV +L ++GQVV V A LDVE +L VLA HP EL + A+
Sbjct: 388 RRFLLNLHLHDVAVVGDELDEYGQVVWVRAGLDVEGDLGARRVLAAHPPELQLELPE*AE 567
Query: 113 LVVEPG 96
++ PG
Sbjct: 568 RLLVPG 585
Score = 52.8 bits (109), Expect(2) = 9e-027
Identities = 25/64 (39%), Positives = 38/64 (59%)
Frame = +2 / -3
Query: 98 RAQRQASPLRHHGRRVQKGAVQVPRRAEDCVPHREGLQPQLPGRACEVCGGRR*HRADGN 277
R + A +R VQ+GA+QV + +P R+ L+P+LPG +V RR HRADG+
Sbjct: 583 RERADAPLIRGARAAVQEGALQVRAWHQGHLPRRDRLEPKLPGHTRQVRRRRRRHRADGD 404
Query: 278 PGQV 289
G++
Sbjct: 403 SGEI 392
>gb|AV917139.1|AV917139 AV917139 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20o17 5', mRNA sequence
Length = 590
Score = 105 bits (225), Expect(3) = 1e-050
Identities = 39/54 (72%), Positives = 46/54 (85%)
Frame = +3 / +1
Query: 9 TVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
TV ITDMNY P+APYHFDLSG AFG LAKPG ND+LRH GI+D++F RV C++P
Sbjct: 37 TVMITDMNYYPVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFP 198
Score = 87.2 bits (184), Expect(3) = 1e-050
Identities = 31/58 (53%), Positives = 44/58 (75%)
Frame = +3 / +1
Query: 300 WKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQF 473
W M+ SWG+IWR+D+ L+ PFSIR+ +ESGK ++A++VIP NWRP+ Y S VQ+
Sbjct: 340 WTRMRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIPKNWRPNTFYRSIVQY 513
Score = 75.3 bits (158), Expect(2) = 4e-022
Identities = 35/55 (63%), Positives = 40/55 (72%)
Frame = -3 / -1
Query: 169 GYLHRTLLNSTSMMPQWRSLSLSPGFAREPKALPLKSKW*GAIGS*FMSVMYTVT 5
G H TL+N SMMP RS SL PG A PKA+PL+SKW*GA G *FMSV+ TV+
Sbjct: 197 GNSHATLVNWMSMMPACRSSSLRPGLASLPKAVPLRSKW*GATG**FMSVIITVS 33
Score = 51.0 bits (105), Expect(2) = 4e-022
Identities = 27/57 (47%), Positives = 31/57 (54%)
Frame = -3 / -1
Query: 469 WTLEV*TASGLQFGGMTSLAMTFLPDSLVRRMEKGPLSALAVSILQMAPQESFMGFH 299
WT+E * GLQF GMT A + LPDSL RME G S QM P +S + H
Sbjct: 509 WTMER*KVLGLQFLGMTLRATSVLPDSLRMRMENGAWRRWLESRRQMDPHDSRIRVH 339
Score = 45.1 bits (92), Expect(3) = 1e-050
Identities = 15/37 (40%), Positives = 27/37 (72%)
Frame = +3 / +1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G K+ FH+E+G N Y+A+LV++ DGD+V +++ +
Sbjct: 199 GLKVGFHVEEGSNAVYMAILVEYENGDGDVVQVDLME 309
>gb|BE060246.3|BE060246 HVSMEg0011L08f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011L08f, mRNA sequence
Length = 544
Score = 101 bits (215), Expect(3) = 2e-049
Identities = 37/49 (75%), Positives = 43/49 (87%)
Frame = +3 / +2
Query: 24 DMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
DMNY P+APYHFDLSG AFG LAKPGLND+LRH GI+D+EF RV C++P
Sbjct: 2 DMNYYPVAPYHFDLSGTAFGRLAKPGLNDRLRHSGIIDIEFTRVPCEFP 148
Score = 88.6 bits (187), Expect(3) = 2e-049
Identities = 33/69 (47%), Positives = 47/69 (68%)
Frame = +3 / +2
Query: 267 LMEIQDKLSAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPD 446
LME + +W M+ SWG++WR+D+ L+ PFSIR+ +ESGK ++A VIP NWRP+
Sbjct: 251 LMESRGPGGGKWTRMRESWGSVWRLDSNHRLQAPFSIRIRNESGKTLVANKVIPANWRPN 430
Query: 447 AVYTSNVQF 473
Y S VQ+
Sbjct: 431 TFYRSFVQY 457
Score = 81.7 bits (172), Expect(3) = 2e-027
Identities = 35/49 (71%), Positives = 39/49 (79%)
Frame = -3 / -2
Query: 169 GYLHRTLLNSTSMMPQWRSLSLSPGFAREPKALPLKSKW*GAIGS*FMS 23
G H T +NS SMMP+WRSLSLSPGFA P A+PL+SKW*GA G *FMS
Sbjct: 147 GNSHGTRVNSMSMMPEWRSLSLSPGFASLPNAVPLRSKW*GATG**FMS 1
Score = 73.9 bits (155), Expect(4) = 5e-021
Identities = 33/47 (70%), Positives = 37/47 (78%)
Frame = -2 / -1
Query: 164 LAPHPSELDVHDAAVAKLVVEPGLCQGAEGLAAQVEVVGSDRLVVHV 24
LA HP ELDV DA VA+ VVEPGLC+ AE AA+VEVVG R+VVHV
Sbjct: 142 LARHPCELDVDDAGVAEPVVEPGLCEPAERRAAEVEVVGRHRVVVHV 2
Score = 67.5 bits (141), Expect(3) = 8e-023
Identities = 30/48 (62%), Positives = 34/48 (70%)
Frame = +2 / +1
Query: 26 HELRAYRSLPLRLERQGLRLPGKARAQRQASPLRHHGRRVQKGAVQVP 169
HEL +LPLR +R G+R KARAQRQA PLRHH RV GAV+VP
Sbjct: 4 HELLPGGALPLRPQRHGVRQARKARAQRQAPPLRHHRHRVHTGAVRVP 147
Score = 48.7 bits (100), Expect(3) = 8e-023
Identities = 24/66 (36%), Positives = 34/66 (51%)
Frame = +2 / +1
Query: 299 VEAHEALLGRHLEDGHCQGAQGPLLHPPHQRVRQEGHRQRRHPAELETRCRLHFQRPILL 478
V+ E ++G L G +LHP QRVRQ+ RQ+ HP +LE + L R + L
Sbjct: 283 VDEDEGVVGLRLAPRLQPPPPGAILHPHPQRVRQDARRQQGHPGQLEAQHLLPLLRAVQL 462
Query: 479 DFEFPS 496
+PS
Sbjct: 463 KSLYPS 480
Score = 44.6 bits (91), Expect(3) = 2e-049
Identities = 17/37 (45%), Positives = 26/37 (70%)
Frame = +3 / +2
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G KI FH+E+ NP Y AVLV++ DGD+V +++ +
Sbjct: 149 GLKIGFHVEEYSNPVYFAVLVEYEDGDGDVVQVDLME 259
Score = 44.1 bits (90), Expect(3) = 2e-027
Identities = 25/56 (44%), Positives = 28/56 (50%)
Frame = -3 / -2
Query: 466 TLEV*TASGLQFGGMTSLAMTFLPDSLVRRMEKGPLSALAVSILQMAPQESFMGFH 299
T E * GLQ GMT LA + LPDSL RME G S Q P +S + H
Sbjct: 450 TKER*KVLGLQLAGMTLLATSVLPDSLRMRMENGAWRRWLESRRQTEPHDSLILVH 283
Score = 34.1 bits (68), Expect(3) = 2e-027
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = -2 / -1
Query: 278 DFHQHDVTVVRHKLHKHGQVVGVAALLDVEHNLLP 174
+ H H V V LH+H +V GV LL VE +L P
Sbjct: 253 EVHLHHVAVAVLVLHQHREVDGVRVLLHVEPDLEP 149
Score = 28.1 bits (55), Expect(3) = 8e-023
Identities = 15/35 (42%), Positives = 16/35 (45%)
Frame = +1 / +3
Query: 175 GRRLCSTSRRAATPTTWPCL*SLWRTTVTSC*WKS 279
G R ST R TP+T C S T T C W S
Sbjct: 150 GSRSGSTWRSTRTPSTSRCWWSTRTATATWCRWTS 254
Score = 28.1 bits (55), Expect(4) = 5e-021
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = -2 / -1
Query: 470 LDVGSVDGIGSPVRRDDV 417
L G+V+G+G PV RDD+
Sbjct: 454 LHEGAVEGVGPPVGRDDL 401
Score = 26.7 bits (52), Expect(4) = 5e-021
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = -3 / -2
Query: 235 TSTAR*LGLQPFSMWNTIFCP 173
TSTA+* G + S WN I P
Sbjct: 210 TSTAK*TGFEYSSTWNPILSP 148
Score = 26.3 bits (51), Expect(4) = 5e-021
Identities = 11/17 (64%), Positives = 11/17 (64%)
Frame = -2 / -1
Query: 344 VHPPDGAPRELHGLPLS 294
V PDGAPR H PLS
Sbjct: 328 VEAPDGAPRLPHPRPLS 278
>gb|BF622288.3|BF622288 HVSMEa0002I16f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0002I16f, mRNA sequence
Length = 851
Score = 98.2 bits (208), Expect(3) = 1e-048
Identities = 36/56 (64%), Positives = 47/56 (83%)
Frame = +3 / +2
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
P TV ITDMNY P++ +HFDLSG AFG++AK G N++LRH GI+D++FRRV C+YP
Sbjct: 251 PETVIITDMNYYPVSRFHFDLSGTAFGAMAKDGRNEELRHAGIIDMQFRRVPCQYP 418
Score = 91.3 bits (193), Expect(3) = 1e-048
Identities = 31/60 (51%), Positives = 46/60 (76%)
Frame = +3 / +2
Query: 291 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQ 470
+ EW PM SWG+IWRMDT + ++GPFS+R+T+ESGK ++A V P +W P+ +Y+S +Q
Sbjct: 572 TGEWVPMTESWGSIWRMDTRRPMQGPFSLRITNESGKTLVADQVNPGDWEPNEIYSSFIQ 751
Score = 75.3 bits (158), Expect(3) = 1e-030
Identities = 35/56 (62%), Positives = 38/56 (67%)
Frame = -3 / -3
Query: 169 GYLHRTLLNSTSMMPQWRSLSLSPGFAREPKALPLKSKW*GAIGS*FMSVMYTVTG 2
GY H TLLN SMMP RS SL P A PKA+PL+SKW G *FMSVM TV+G
Sbjct: 417 GYWHGTLLNCMSMMPACRSSSLRPSLAMAPKAVPLRSKWKRDTG**FMSVMITVSG 250
Score = 65.2 bits (136), Expect(3) = 1e-030
Identities = 31/58 (53%), Positives = 36/58 (62%)
Frame = -3 / -3
Query: 469 WTLEV*TASGLQFGGMTSLAMTFLPDSLVRRMEKGPLSALAVSILQMAPQESFMGFHS 296
W E+* + G Q G+T A + LPDSLV R EKGP VSI QM PQ+S MG HS
Sbjct: 750 WMKEL*ISLGSQSPGLTWSATSVLPDSLVMRSEKGPCMGRRVSIRQMEPQDSVMGTHS 577
Score = 61.1 bits (127), Expect(3) = 3e-021
Identities = 29/53 (54%), Positives = 32/53 (60%)
Frame = +2 / +1
Query: 11 GVHH*HELRAYRSLPLRLERQGLRLPGKARAQRQASPLRHHGRRVQKGAVQVP 169
G HH HEL LPLR +R LR G+ R QR+A RHH VQKGAV VP
Sbjct: 259 GDHHGHELLPGVPLPLRPQRHRLRRHGQGRPQRRAPARRHHRHAVQKGAVPVP 417
Score = 51.9 bits (107), Expect(3) = 3e-021
Identities = 24/57 (42%), Positives = 29/57 (50%)
Frame = +2 / +1
Query: 299 VEAHEALLGRHLEDGHCQGAQGPLLHPPHQRVRQEGHRQRRHPAELETRCRLHFQRP 469
V AH+ +LG HL DGH GPLL HQRVRQ+ R+ L + L P
Sbjct: 580 VGAHDRVLGLHLADGHTAPHAGPLLAAHHQRVRQDARRRPGQSRRLGAQRNLQLLHP 750
Score = 42.3 bits (86), Expect(3) = 1e-048
Identities = 14/30 (46%), Positives = 21/30 (70%)
Frame = +3 / +2
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDI 263
G + FH++ G NP YLA+LV++ DGD+
Sbjct: 419 GLTVTFHVQHGSNPYYLAILVEYENGDGDV 508
Score = 30.4 bits (60), Expect(3) = 1e-030
Identities = 15/33 (45%), Positives = 18/33 (54%)
Frame = -2 / -2
Query: 272 HQHDVTVVRHKLHKHGQVVGVAALLDVEHNLLP 174
H V V LH+ GQVVGV +L VE + P
Sbjct: 517 HLVHVAVAVLVLHQDGQVVGVGTVLHVERHRQP 419
Score = 25.8 bits (50), Expect(3) = 3e-021
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +1 / +3
Query: 190 STSRRAATPTTWPCL*SLWRTTVTSC*W 273
ST TPTTWP S T T W
Sbjct: 435 STCSTVPTPTTWPSWWSTRTATATWTRW 518
>gb|BQ759881.1|BQ759881 EBpi05_SQ004_P16_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ004_P16 5', mRNA sequence
Length = 500
Score = 134 bits (287), Expect(2) = 1e-048
Identities = 49/100 (49%), Positives = 70/100 (70%)
Frame = +3 / +2
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDTAK 353
G K+ FH+E+G NP Y AVLV++ DGD+V +++ + S W PM+ SWG+IWR+D+
Sbjct: 122 GLKVTFHVEQGSNPVYFAVLVEYEDGDGDVVQVDLMEANSGTWTPMRESWGSIWRLDSGH 301
Query: 354 ALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQF 473
L+ PFS+R+T+ESGK ++A VIP NW P Y S VQ+
Sbjct: 302 RLQAPFSMRITNESGKTLVADKVIPANWAPSTFYRSIVQY 421
Score = 79.0 bits (166), Expect(2) = 1e-048
Identities = 27/40 (67%), Positives = 37/40 (92%)
Frame = +3 / +2
Query: 51 YHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
YHFDLSG AFG++AKPGL++KLRH GI+D++F+RV C++P
Sbjct: 2 YHFDLSGTAFGAMAKPGLSEKLRHSGIIDIQFKRVPCEFP 121
Score = 68.4 bits (143), Expect(2) = 1e-022
Identities = 36/99 (36%), Positives = 45/99 (45%)
Frame = -3 / -3
Query: 469 WTLEV*TASGLQFGGMTSLAMTFLPDSLVRRMEKGPLSALAVSILQMAPQESFMGFHSAD 290
WT+E * G Q GMT A + LPDSLV RMEKG S Q+ P +S +G H +
Sbjct: 417 WTMER*KVLGAQLAGMTLSATSVLPDSLVMRMEKGAWRRWPESSRQIDPHDSRIGVHVPE 238
Query: 289 NLXXXXXXXXXXXSATNFTSTAR*LGLQPFSMWNTIFCP 173
++ TSTA+ G P S W P
Sbjct: 237 LASMRSTCTTSPSPSSYSTSTAKYTGFDPCSTWKVTLRP 121
Score = 57.9 bits (120), Expect(2) = 1e-022
Identities = 24/39 (61%), Positives = 27/39 (69%)
Frame = -3 / -3
Query: 169 GYLHRTLLNSTSMMPQWRSLSLSPGFAREPKALPLKSKW 53
G H TLLN SMMP+ RS SL PG A P A+PL+SKW
Sbjct: 120 GNSHGTLLNWMSMMPECRSFSLRPGLAMAPNAVPLRSKW 4
>gb|CB880524.1|CB880524 HM06N05w HM Hordeum vulgare subsp. vulgare cDNA clone HM06N05
3-PRIME, mRNA sequence
Length = 545
Score = 116 bits (247), Expect(2) = 2e-047
Identities = 44/59 (74%), Positives = 50/59 (84%)
Frame = +3 / -2
Query: 297 EWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQF 473
+W PM LSWGAIWR D A A+K PFSIRLTSESGKK+IA+DVIP NW+PD VY SN+QF
Sbjct: 391 KWIPMTLSWGAIWRWDGANAIKAPFSIRLTSESGKKLIAQDVIPANWKPDTVYQSNIQF 215
Score = 93.2 bits (197), Expect(2) = 2e-047
Identities = 36/47 (76%), Positives = 40/47 (85%)
Frame = +3 / -2
Query: 147 RRVRCKYPAGQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDK 287
RRVRCKY G K+ FH+E G NPNYLA+LVKFVADDGDIV MEIQ+K
Sbjct: 544 RRVRCKYAPGTKVTFHVETGSNPNYLAILVKFVADDGDIVQMEIQEK 404
Score = 87.2 bits (184), Expect(2) = 2e-026
Identities = 39/59 (66%), Positives = 43/59 (72%)
Frame = -3 / +3
Query: 475 *NWTLEV*TASGLQFGGMTSLAMTFLPDSLVRRMEKGPLSALAVSILQMAPQESFMGFH 299
*NW + *T SG Q GMTS AM+FLPDSLVRRMEKG L ALA S LQMAP + +G H
Sbjct: 213 *NWIFDW*TVSGFQLAGMTSWAMSFLPDSLVRRMEKGALIALAPSHLQMAPHDKVIGIH 389
Score = 85.4 bits (180), Expect(2) = 2e-025
Identities = 35/61 (57%), Positives = 42/61 (68%)
Frame = +2 / -1
Query: 299 VEAHEALLGRHLEDGHCQGAQGPLLHPPHQRVRQEGHRQRRHPAELETRCRLHFQRPILL 478
V+ + ++GRHLE G Q QGPL HPPHQRVRQE HR RHP +LE R RL + P+L
Sbjct: 389 VDPDDLVVGRHLEVGRRQCDQGPLFHPPHQRVRQEAHRPGRHPRQLEARHRLPVKYPVLE 210
Query: 479 D 481
D
Sbjct: 209 D 207
Score = 51.9 bits (107), Expect(2) = 2e-026
Identities = 25/48 (52%), Positives = 32/48 (66%)
Frame = -2 / +2
Query: 293 RQLVLDFHQHDVTVVRHKLHKHGQVVGVAALLDVEHNLLPGGVLAPHP 150
R+ +L+ H HDV VV +L ++GQVV V A LDVE +L VLA HP
Sbjct: 398 RRFLLNLHLHDVAVVGDELDEYGQVVWVRAGLDVEGDLGARRVLAAHP 541
Score = 50.1 bits (103), Expect(2) = 2e-025
Identities = 25/48 (52%), Positives = 27/48 (56%)
Frame = +1 / -3
Query: 151 GCGASTPPGRRLCSTSRRAATPTTWPCL*SLWRTTVTSC*WKSRTSCR 294
GC AST R STSR A T TTWP S TT TSC W+ R + R
Sbjct: 540 GCAASTRLAPRSPSTSRPARTQTTWPYSSSSSPTTATSCRWRFRRNRR 397
>gb|BF254126.2|BF254126 HVSMEf0003B13f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0003B13f, mRNA sequence
Length = 789
Score = 105 bits (225), Expect(3) = 2e-045
Identities = 39/54 (72%), Positives = 46/54 (85%)
Frame = +3 / +2
Query: 9 TVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
TV ITDMNY P+APYHFDLSG AFG LAKPG ND+LRH GI+D++F RV C++P
Sbjct: 176 TVMITDMNYYPVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFP 337
Score = 70.3 bits (147), Expect(3) = 2e-045
Identities = 26/55 (47%), Positives = 39/55 (70%)
Frame = +3 / +2
Query: 300 WKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSN 464
W M+ SWG IWR+D+ L+ PFSIR+ +ESG+ ++A+ VIP RP+ +Y S+
Sbjct: 479 WTRMRESWGFIWRLDSNHRLQAPFSIRIRNESGQTLVAR*VIPKKGRPNTLYCSS 643
Score = 45.1 bits (92), Expect(3) = 2e-045
Identities = 15/37 (40%), Positives = 27/37 (72%)
Frame = +3 / +2
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G K+ FH+E+G N Y+A+LV++ DGD+V +++ +
Sbjct: 338 GLKVGFHVEEGSNAVYMAILVEYENGDGDVVQVDLME 448
>gb|CK123980.1|CK123980 BES1824106f16 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010F166 5-PRIME, mRNA sequence
Length = 750
Score = 105 bits (225), Expect(3) = 4e-044
Identities = 39/54 (72%), Positives = 46/54 (85%)
Frame = +3 / +1
Query: 9 TVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
TV ITDMNY P+APYHFDLSG AFG LAKPG ND+LRH GI+D++F RV C++P
Sbjct: 232 TVMITDMNYYPVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFP 393
Score = 65.7 bits (137), Expect(3) = 4e-044
Identities = 23/43 (53%), Positives = 34/43 (79%)
Frame = +3 / +1
Query: 300 WKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIP 428
W M+ SWG+IWR+D+ L+ PFSIR+ +ESGK ++A++VIP
Sbjct: 535 WTRMRESWGSIWRLDSNHRLQAPFSIRIRNESGKTLVARNVIP 663
Score = 45.1 bits (92), Expect(3) = 4e-044
Identities = 15/37 (40%), Positives = 27/37 (72%)
Frame = +3 / +1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G K+ FH+E+G N Y+A+LV++ DGD+V +++ +
Sbjct: 394 GLKVGFHVEEGSNAVYMAILVEYENGDGDVVQVDLME 504
>gb|BF260026.3|BF260026 HVSMEf0020O13f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020O13f, mRNA sequence
Length = 535
Score = 134 bits (287), Expect(2) = 6e-044
Identities = 51/100 (51%), Positives = 74/100 (74%)
Frame = +3 / +1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDTAK 353
G KI FH+ +G N YLAVL+++ DGD++ +++++ S W M+ SWG+IWRMD+
Sbjct: 103 GLKINFHVVEGSNAVYLAVLIEYEDMDGDVIQVDMKEANSGSWMAMRESWGSIWRMDSNH 282
Query: 354 ALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQF 473
L+GPFS+R+TS+SGKK++A +VIP NWRP+ Y S VQF
Sbjct: 283 RLQGPFSMRITSDSGKKLVANNVIPANWRPNTDYRSFVQF 402
Score = 82.6 bits (174), Expect(2) = 2e-025
Identities = 41/100 (41%), Positives = 51/100 (51%)
Frame = -3 / -3
Query: 472 NWTLEV*TASGLQFGGMTSLAMTFLPDSLVRRMEKGPLSALAVSILQMAPQESFMGFHSA 293
NWT E *+ GLQ GMT LA +FLP+SLV RMEKGP SI QM P +S + H
Sbjct: 401 NWTKER*SVLGLQLAGMTLLATSFLPESLVMRMEKGPWRRWLESIRQMDPHDSRIAIHDP 222
Query: 292 DNLXXXXXXXXXXXSATNFTSTAR*LGLQPFSMWNTIFCP 173
+ ++ STAR L+P + W I P
Sbjct: 221 ELASFMSTWITSPSMSSYSISTARYTALEPSTTWKLILRP 102
Score = 76.7 bits (161), Expect(2) = 1e-022
Identities = 40/102 (39%), Positives = 53/102 (51%)
Frame = +2 / +3
Query: 173 RAEDCVPHREGLQPQLPGRACEVCGGRR*HRADGNPGQVVG*VEAHEALLGRHLEDGHCQ 352
R +D +P GLQ +PG A V G R G+ G +G V+ ++G HL DG
Sbjct: 102 RPQDQLPRGGGLQRGVPGGADRVRGHGRRRDPGGHEGGQLGVVDGDARVVGIHLADGLQP 281
Query: 353 GAQGPLLHPPHQRVRQEGHRQRRHPAELETRCRLHFQRPILL 478
G LH HQR+RQE Q+ HP +LE + RL RP+ L
Sbjct: 282 PPPGAFLHAHHQRLRQEAGGQQCHPGQLEAQHRLPLLRPVQL 407
Score = 63.4 bits (132), Expect(2) = 6e-044
Identities = 23/34 (67%), Positives = 29/34 (85%)
Frame = +3 / +1
Query: 69 GKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
G AFG+LAK GLN+KLRH GI+D++FRRV C +P
Sbjct: 1 GTAFGALAKSGLNEKLRHSGIIDIQFRRVPCNFP 102
Score = 53.3 bits (110), Expect(2) = 2e-025
Identities = 22/33 (66%), Positives = 24/33 (72%)
Frame = -3 / -3
Query: 169 GYLHRTLLNSTSMMPQWRSLSLSPGFAREPKAL 71
G LH TLLN SMMP+WRS SL P AR PKA+
Sbjct: 101 GKLHGTLLNWMSMMPEWRSFSLRPDLARAPKAV 3
Score = 49.6 bits (102), Expect(2) = 1e-022
Identities = 20/33 (60%), Positives = 24/33 (72%)
Frame = +2 / +3
Query: 74 GLRLPGKARAQRQASPLRHHGRRVQKGAVQVPR 172
GLR PG+ QR+A PL HH VQ+GAVQ+PR
Sbjct: 6 GLRRPGQVGPQREAPPLGHHRHPVQEGAVQLPR 104
>gb|BQ661443.1|BQ661443 HM03I08u HM Hordeum vulgare subsp. vulgare cDNA clone HM03I08
3-PRIME, mRNA sequence
Length = 520
Score = 116 bits (247), Expect(2) = 1e-043
Identities = 44/59 (74%), Positives = 50/59 (84%)
Frame = +3 / -3
Query: 297 EWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQF 473
+W PM LSWGAIWR D A A+K PFSIRLTSESGKK+IA+DVIP NW+PD VY SN+QF
Sbjct: 380 KWIPMTLSWGAIWRWDGANAIKAPFSIRLTSESGKKLIAQDVIPANWKPDTVYQSNIQF 204
Score = 87.2 bits (184), Expect(2) = 3e-024
Identities = 39/59 (66%), Positives = 43/59 (72%)
Frame = -3 / +1
Query: 475 *NWTLEV*TASGLQFGGMTSLAMTFLPDSLVRRMEKGPLSALAVSILQMAPQESFMGFH 299
*NW + *T SG Q GMTS AM+FLPDSLVRRMEKG L ALA S LQMAP + +G H
Sbjct: 202 *NWIFDW*TVSGFQLAGMTSWAMSFLPDSLVRRMEKGALIALAPSHLQMAPHDKVIGIH 378
Score = 87.2 bits (184), Expect(2) = 7e-024
Identities = 36/61 (59%), Positives = 43/61 (70%)
Frame = +2 / -2
Query: 299 VEAHEALLGRHLEDGHCQGAQGPLLHPPHQRVRQEGHRQRRHPAELETRCRLHFQRPILL 478
V+ + ++GRHLE G Q QGPLLHPPHQRVRQE HR RHP +LE R RL + P+L
Sbjct: 378 VDPDDLVVGRHLEVGRRQCDQGPLLHPPHQRVRQEAHRPGRHPRQLEARHRLPVKYPVLE 199
Query: 479 D 481
D
Sbjct: 198 D 196
Score = 80.3 bits (169), Expect(2) = 1e-043
Identities = 31/42 (73%), Positives = 35/42 (83%)
Frame = +3 / -3
Query: 162 KYPAGQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDK 287
KY G K+ FH+E G NPNYLA+LVKFVADDGDIV MEIQ+K
Sbjct: 518 KYAPGTKVTFHVETGSNPNYLAILVKFVADDGDIVQMEIQEK 393
Score = 44.1 bits (90), Expect(2) = 3e-024
Identities = 20/38 (52%), Positives = 27/38 (71%)
Frame = -2 / +3
Query: 293 RQLVLDFHQHDVTVVRHKLHKHGQVVGVAALLDVEHNL 180
R+ +L+ H HDV VV +L ++GQVV V A LDVE +L
Sbjct: 387 RRFLLNLHLHDVAVVGDELDEYGQVVWVRAGLDVEGDL 500
Score = 43.2 bits (88), Expect(2) = 7e-024
Identities = 23/45 (51%), Positives = 25/45 (55%)
Frame = +1 / -1
Query: 160 ASTPPGRRLCSTSRRAATPTTWPCL*SLWRTTVTSC*WKSRTSCR 294
AST R STSR A T TTWP S TT TSC W+ R + R
Sbjct: 520 ASTRLAPRSPSTSRPARTQTTWPYSSSSSPTTATSCRWRFRRNRR 386
>gb|BM442635.2|BM442635 EBan01_SQ003_L19_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_L19 5', mRNA sequence
Length = 411
Score = 173 bits (373), Expect = 8e-043
Identities = 66/119 (55%), Positives = 88/119 (73%)
Frame = +3 / +3
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYPAGQK 182
P V ITD N EPIA YHFDLSG AFG++AK G KLR G ++++FRRV+CKYP G K
Sbjct: 54 PTIVTITDKNEEPIAAYHFDLSGHAFGTMAKKGQEQKLRDAGEVEIKFRRVKCKYPEGTK 233
Query: 183 IVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDTAKAL 359
+ FH+EKG +PNYLA+++KF+ DGD+V ++I+ K +W +K SWGA+WR+DT L
Sbjct: 234 VNFHVEKGSSPNYLALVIKFLQGDGDVVAVDIKPKGKDKWIELKESWGAVWRIDTPDKL 410
>gb|BI956772.1|BI956772 HVSMEn0005E06f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005E06f, mRNA sequence
Length = 796
Score = 105 bits (223), Expect(2) = 1e-042
Identities = 38/56 (67%), Positives = 49/56 (87%)
Frame = +3 / +1
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
P TV ITDMNY P+A YHFDLSG AFG++AKPGL++KLRH GI+D++F+RV C++P
Sbjct: 274 PETVVITDMNYYPVAKYHFDLSGTAFGAMAKPGLSEKLRHSGIIDIQFKRVPCEFP 441
Score = 88.1 bits (186), Expect(2) = 1e-042
Identities = 32/78 (41%), Positives = 51/78 (65%)
Frame = +3 / +1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDTAK 353
G K+ FH+E+G NP Y AVLV++ DGD+V +++ + S W PM+ SWG+I R+D+
Sbjct: 442 GLKVTFHVEQGSNPVYFAVLVEYXDGDGDMVQVDLMEANSCTWTPMRESWGSILRLDSGH 621
Query: 354 ALKGPFSIRLTSESGKKV 407
+ P S L +E G+++
Sbjct: 622 GWRPPLSFALXNEGGREL 675
>gb|CV058074.1|CV058074 BNEL34a7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL34a7 5' similar to Oryza sativa
beta-expansin, mRNA sequence
Length = 630
Score = 91.3 bits (193), Expect(3) = 3e-041
Identities = 33/66 (50%), Positives = 49/66 (74%)
Frame = +3 / +3
Query: 291 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQ 470
+ +W PMK SWG+IWRMD + GPFS+R+T+ESGK ++A VI +W+P+ Y+S VQ
Sbjct: 270 TGQWVPMKESWGSIWRMDAHHPMHGPFSLRVTNESGKTLVADQVIXGDWKPNKTYSSLVQ 449
Query: 471 FY*TLN 488
F+* ++
Sbjct: 450 FH*LID 467
Score = 69.8 bits (146), Expect(3) = 3e-041
Identities = 25/39 (64%), Positives = 34/39 (87%)
Frame = +3 / +3
Query: 54 HFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
HFDLSG AFG++AK G ND+LRH GI++++F+RV C+YP
Sbjct: 3 HFDLSGTAFGAMAKYGRNDELRHAGIINMQFKRVPCQYP 119
Score = 62.5 bits (130), Expect(2) = 5e-021
Identities = 31/60 (51%), Positives = 35/60 (58%)
Frame = -3 / -3
Query: 478 **NWTLEV*TASGLQFGGMTSLAMTFLPDSLVRRMEKGPLSALAVSILQMAPQESFMGFH 299
**NWT E+* G Q MT A + PDSLV R E GP SILQ+ P +SFMG H
Sbjct: 457 **NWTREL*VLLGFQSPXMTWSATSVFPDSLVTRNENGPCMGWWASILQIDPHDSFMGTH 278
Score = 60.2 bits (125), Expect(2) = 5e-021
Identities = 24/39 (61%), Positives = 27/39 (69%)
Frame = -3 / -3
Query: 169 GYLHRTLLNSTSMMPQWRSLSLSPGFAREPKALPLKSKW 53
GY H TLLN +MP WRS SL P A PKA+PL+SKW
Sbjct: 118 GYWHGTLLNCMLIMPAWRSSSLRPYLAMAPKAVPLRSKW 2
Score = 46.0 bits (94), Expect(3) = 3e-041
Identities = 15/37 (40%), Positives = 26/37 (70%)
Frame = +3 / +3
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G + FH+E+G NP Y+A+LV++ DGD+ ++I +
Sbjct: 120 GLTVTFHVEEGSNPFYMAILVEYENGDGDVKQLDIME 230
>gb|CK122737.1|CK122737 BES1824105j22 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010J225 5-PRIME, mRNA sequence
Length = 776
Score = 105 bits (225), Expect(3) = 5e-041
Identities = 39/54 (72%), Positives = 46/54 (85%)
Frame = +3 / +1
Query: 9 TVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
TV ITDMNY P+APYHFDLSG AFG LAKPG ND+LRH GI+D++F RV C++P
Sbjct: 349 TVMITDMNYYPVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFP 510
Score = 55.1 bits (114), Expect(3) = 5e-041
Identities = 20/44 (45%), Positives = 30/44 (68%)
Frame = +3 / +3
Query: 291 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDV 422
+ W M SWG+IW +D+ L+ P SIR+ +ESGKK+ A++V
Sbjct: 645 AGRWTRMPESWGSIWGLDSXNRLQXPXSIRIRNESGKKLXARNV 776
Score = 45.1 bits (92), Expect(3) = 5e-041
Identities = 15/37 (40%), Positives = 27/37 (72%)
Frame = +3 / +1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G K+ FH+E+G N Y+A+LV++ DGD+V +++ +
Sbjct: 511 GLKVGFHVEEGSNAVYMAILVEYENGDGDVVQVDLME 621
>gb|BE454367.2|BE454367 HVSMEh0093N18f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0093N18f, mRNA sequence
Length = 870
Score = 106 bits (227), Expect(3) = 2e-040
Identities = 40/53 (75%), Positives = 46/53 (86%)
Frame = +3 / +1
Query: 12 VYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
V ITDMNY P+APYHFDLSG AFG LAKPGLND+LRH GI+D+EF RV C++P
Sbjct: 1 VIITDMNYYPVAPYHFDLSGTAFGRLAKPGLNDRLRHSGIIDIEFTRVPCEFP 159
Score = 85.8 bits (181), Expect(3) = 2e-023
Identities = 37/51 (72%), Positives = 41/51 (80%)
Frame = -3 / -2
Query: 169 GYLHRTLLNSTSMMPQWRSLSLSPGFAREPKALPLKSKW*GAIGS*FMSVM 17
G H T +NS SMMP+WRSLSLSPGFA P A+PL+SKW*GA G *FMSVM
Sbjct: 158 GNSHGTRVNSMSMMPEWRSLSLSPGFASLPNAVPLRSKW*GATG**FMSVM 6
Score = 52.8 bits (109), Expect(3) = 2e-040
Identities = 19/59 (32%), Positives = 33/59 (55%)
Frame = +3 / +1
Query: 267 LMEIQDKLSAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRP 443
LME + +W M+ SWG++WR++ L+ PF I++ + G+ + +P WRP
Sbjct: 262 LMESRGPGGGKWTRMRESWGSVWRLNCNHRLQAPFCIQVRDKCGETLGGSMGVPGRWRP 438
Score = 44.6 bits (91), Expect(3) = 2e-040
Identities = 17/37 (45%), Positives = 26/37 (70%)
Frame = +3 / +1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G KI FH+E+ NP Y AVLV++ DGD+V +++ +
Sbjct: 160 GLKIGFHVEEYSNPVYFAVLVEYEDGDGDVVQVDLME 270
Score = 34.1 bits (68), Expect(3) = 2e-023
Identities = 16/35 (45%), Positives = 20/35 (57%)
Frame = -2 / -1
Query: 278 DFHQHDVTVVRHKLHKHGQVVGVAALLDVEHNLLP 174
+ H H V V LH+H +V GV LL VE +L P
Sbjct: 264 EVHLHHVAVAVLVLHQHREVDGVRVLLHVEPDLEP 160
Score = 26.7 bits (52), Expect(3) = 2e-023
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = -2 / -1
Query: 347 SVHPPDGAPRELHGLPLS 294
+V PDGAPR H PLS
Sbjct: 342 AVQAPDGAPRLPHPRPLS 289
>gb|BQ468475.1|BQ468475 HM01F21T HM Hordeum vulgare subsp. vulgare cDNA clone HM01F21
5-PRIME, mRNA sequence
Length = 650
Score = 161 bits (346), Expect = 4e-039
Identities = 63/92 (68%), Positives = 74/92 (80%)
Frame = +3 / +2
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYPAGQK 182
P+TV+ITD NYEPIAPYH DLSGKAFG++A G LR G ++++FRRVRCKY G K
Sbjct: 374 PITVFITDKNYEPIAPYHIDLSGKAFGAMATAGNEQTLRSFGELELQFRRVRCKYAPGTK 553
Query: 183 IVFHIEKGCNPNYLAVLVKFVADDGDIVLMEI 278
+ FH+E G NPNYLA+LVKFVADDGDIV MEI
Sbjct: 554 VTFHVETGSNPNYLAILVKFVADDGDIVQMEI 649
>gb|BF628930.2|BF628930 HVSMEb0009C23f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0009C23f, mRNA sequence
Length = 762
Score = 103 bits (219), Expect(2) = 6e-039
Identities = 39/54 (72%), Positives = 46/54 (85%)
Frame = +3 / +3
Query: 9 TVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
TV ITDMNY P+A YHFDLSG AFG+LAK GLN+KLRH GI+D++FRRV C +P
Sbjct: 402 TVMITDMNYYPVAQYHFDLSGTAFGALAKSGLNEKLRHSGIIDIQFRRVPCNFP 563
Score = 81.7 bits (172), Expect(2) = 8e-023
Identities = 36/55 (65%), Positives = 41/55 (74%)
Frame = -3 / -3
Query: 169 GYLHRTLLNSTSMMPQWRSLSLSPGFAREPKALPLKSKW*GAIGS*FMSVMYTVT 5
G LH TLLN SMMP+WRS SL P AR PKA+PL+SKW A G *FMSV+ TV+
Sbjct: 562 GKLHGTLLNWMSMMPEWRSFSLRPDLARAPKAVPLRSKWYWATG**FMSVIITVS 398
Score = 77.6 bits (163), Expect(2) = 6e-039
Identities = 29/65 (44%), Positives = 44/65 (67%)
Frame = +3 / +3
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDTAK 353
G KI FH+ +G N YLAVL+++ DGD++ ++++ S W M+ SWG+IWRMD+
Sbjct: 564 GLKINFHVVEGSNAVYLAVLIEYEDMDGDVIQVDMKGANSGSWMAMRESWGSIWRMDSNH 743
Query: 354 ALKGP 368
L+GP
Sbjct: 744 RLQGP 758
Score = 45.1 bits (92), Expect(2) = 8e-023
Identities = 28/65 (43%), Positives = 31/65 (47%)
Frame = -2 / -2
Query: 344 VHPPDGAPRELHGLPLSRQLVLDFHQHDVTVVRHKLHKHGQVVGVAALLDVEHNLLPGGV 165
VHPPDG+PR H P R L H V V L +H QV V AL VE +L G V
Sbjct: 734 VHPPDGSPRLAHRHPRPRVGSLHVHLDHVAVHVLVLDQHRQVHRVGALHHVEVDLEAGEV 555
Query: 164 LAPHP 150
P
Sbjct: 554 ARHPP 540
>gb|BM097618.2|BM097618 EBem04_SQ002_D15_R embryo, 12 DPA, no treatment, cv Optic, EBem04
Hordeum vulgare subsp. vulgare cDNA clone
EBem04_SQ002_D15 5', mRNA sequence
Length = 477
Score = 99.6 bits (211), Expect(3) = 7e-039
Identities = 37/56 (66%), Positives = 47/56 (83%)
Frame = +3 / +3
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
P TV ITDMNY P++ YHFDLSG AFG++AK G ND+LRH GI+D++F+RV C+YP
Sbjct: 75 PETVIITDMNYYPVSRYHFDLSGTAFGAMAKYGRNDELRHAGIIDMQFKRVPCQYP 242
Score = 53.8 bits (111), Expect(3) = 7e-039
Identities = 17/29 (58%), Positives = 24/29 (82%)
Frame = +3 / +3
Query: 300 WKPMKLSWGAIWRMDTAKALKGPFSIRLT 386
W PM+ SWG+IWR+D + L+GPFS+R+T
Sbjct: 387 WTPMRESWGSIWRLDRLRPLQGPFSLRVT 473
Score = 45.5 bits (93), Expect(3) = 7e-039
Identities = 15/37 (40%), Positives = 25/37 (67%)
Frame = +3 / +3
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G + F ++ G NPNYLA+LV++ DGD+ ++I +
Sbjct: 243 GLTVTFRVQHGSNPNYLAILVEYEDGDGDVAQVDIME 353
>gb|BM442090.2|BM442090 EBan01_SQ002_C16_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_C16 5', mRNA sequence
Length = 389
Score = 159 bits (342), Expect = 2e-038
Identities = 57/104 (54%), Positives = 78/104 (75%)
Frame = +3 / +2
Query: 144 FRRVRCKYPAGQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSW 323
FRRV+CKYP G K+ FH+EKG +PNYLA+++KF+ DGD+V ++I+ K +W +K SW
Sbjct: 2 FRRVKCKYPEGTKVNFHVEKGSSPNYLALVIKFLQGDGDVVAVDIKPKGKDKWIELKESW 181
Query: 324 GAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVY 455
GA+WR+DT L GPFS+R T+E G K +A+DVIP W+PD Y
Sbjct: 182 GAVWRIDTPDKLIGPFSVRYTTEGGTKTVAEDVIPEGWKPDTFY 313
>gb|BI959138.1|BI959138 HVSMEn0018F10f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0018F10f, mRNA sequence
Length = 669
Score = 105 bits (223), Expect(3) = 2e-038
Identities = 38/56 (67%), Positives = 49/56 (87%)
Frame = +3 / +3
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
P TV ITDMNY P+A YHFDLSG AFG++AKPGL++KLRH GI+D++F+RV C++P
Sbjct: 291 PETVVITDMNYYPVAKYHFDLSGTAFGAMAKPGLSEKLRHSGIIDIQFKRVPCEFP 458
Score = 53.8 bits (111), Expect(3) = 2e-038
Identities = 19/43 (44%), Positives = 29/43 (67%)
Frame = +3 / +3
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEW 302
G K+ FH+E+G NP Y AVLV++ DGD+V +++ + S W
Sbjct: 459 GLKVTFHVEQGSNPVYFAVLVEYEDGDGDLVQVDLMEANSGTW 587
Score = 38.6 bits (78), Expect(3) = 2e-038
Identities = 12/24 (50%), Positives = 19/24 (79%)
Frame = +3 / +1
Query: 306 PMKLSWGAIWRMDTAKALKGPFSI 377
PM+ SWG+IWR+++ L+ PFS+
Sbjct: 592 PMRESWGSIWRVESGHRLQAPFSM 663
>gb|BQ753409.1|BQ753409 EBan01_SQ004_D03_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_D03 5', mRNA sequence
Length = 677
Score = 159 bits (341), Expect = 2e-038
Identities = 61/109 (55%), Positives = 81/109 (74%)
Frame = +3 / +2
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYPAGQK 182
P V ITD N EPIA YHFDLSG AFG++AK G KLR G ++++FRRV+CKYP G K
Sbjct: 350 PTIVTITDKNEEPIAAYHFDLSGHAFGTMAKKGQEQKLRDAGEVEIKFRRVKCKYPEGTK 529
Query: 183 IVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGA 329
+ FH+EKG +PNYLA+++KF+ DGD+V ++I+ K +W +K SWGA
Sbjct: 530 VNFHVEKGSSPNYLALVIKFLQGDGDVVAVDIKPKGKDKWIELKESWGA 676
>gb|BF253787.2|BF253787 HVSMEf0002C01f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0002C01f, mRNA sequence
Length = 823
Score = 103 bits (220), Expect(3) = 6e-038
Identities = 38/53 (71%), Positives = 45/53 (84%)
Frame = +3 / +3
Query: 12 VYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
V ITDMNY P+APYHFDLSG AFG LAKPG ND+LRH GI+D++F RV C++P
Sbjct: 3 VMITDMNYYPVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFP 161
Score = 47.3 bits (97), Expect(3) = 6e-038
Identities = 18/43 (41%), Positives = 27/43 (62%)
Frame = +3 / +3
Query: 300 WKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIP 428
W PM SWG+IWR+ ++ P+SIR+ E G +I + +IP
Sbjct: 303 WTPMLKSWGSIWRLYYNHGIQTPYSIRIRCEFG*TLIMRILIP 431
Score = 44.6 bits (91), Expect(3) = 6e-038
Identities = 15/37 (40%), Positives = 27/37 (72%)
Frame = +3 / +3
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G K+ FH+E+G N Y+A+LV++ DGD+V +++ +
Sbjct: 162 GLKVGFHVEEGSNAVYMAILVEYENGDGDVVHVDLME 272
>gb|BQ465138.1|BQ465138 HU02M06r HU Hordeum vulgare subsp. vulgare cDNA clone HU02M06
5-PRIME, mRNA sequence
Length = 623
Score = 105 bits (225), Expect(3) = 2e-037
Identities = 39/54 (72%), Positives = 46/54 (85%)
Frame = +3 / +2
Query: 9 TVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
TV ITDMNY P+APYHFDLSG AFG LAKPG ND+LRH GI+D++F RV C++P
Sbjct: 197 TVMITDMNYYPVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFP 358
Score = 45.1 bits (92), Expect(3) = 2e-037
Identities = 15/37 (40%), Positives = 27/37 (72%)
Frame = +3 / +2
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G K+ FH+E+G N Y+A+LV++ DGD+V +++ +
Sbjct: 359 GLKVGFHVEEGSNAVYMAILVEYENGDGDVVQVDLME 469
Score = 43.2 bits (88), Expect(3) = 2e-037
Identities = 15/30 (50%), Positives = 24/30 (80%)
Frame = +3 / +3
Query: 333 WRMDTAKALKGPFSIRLTSESGKKVIAKDV 422
WR+D+ L+ PFSIR+ +ESGK ++A++V
Sbjct: 534 WRLDSNHRLQAPFSIRIRNESGKTLVARNV 623
>gb|CB883976.1|CB883976 HM06N05r HM Hordeum vulgare subsp. vulgare cDNA clone HM06N05
5-PRIME, mRNA sequence
Length = 616
Score = 155 bits (334), Expect = 2e-037
Identities = 60/88 (68%), Positives = 71/88 (80%)
Frame = +3 / +2
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYPAGQK 182
P+TV+ITD NYEPIAPYH DLSGKAFG++A G LR G ++++FRRVRCKY G K
Sbjct: 353 PITVFITDKNYEPIAPYHIDLSGKAFGAMATAGNEQTLRSFGELELQFRRVRCKYAPGTK 532
Query: 183 IVFHIEKGCNPNYLAVLVKFVADDGDIV 266
+ FH+E G NPNYLA+LVKFVADDGDIV
Sbjct: 533 VTFHVETGSNPNYLAILVKFVADDGDIV 616
>gb|BI950777.1|BI950777 HVSMEl0023C15f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023C15f, mRNA sequence
Length = 986
Score = 106 bits (227), Expect(2) = 4e-037
Identities = 39/100 (39%), Positives = 61/100 (61%)
Frame = +3 / +3
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDTAK 353
G I F +++G NP Y L++F DDGD+ + +++ S W M WGA+WR++
Sbjct: 285 GVNIAFKVDQGSNPFYFKTLIEFEDDDGDLKAVALKEAGSGAWTQMTQDWGALWRLNNGN 464
Query: 354 ALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQF 473
L+ PFS++LTS+SG+K++ +VI NW A Y S V +
Sbjct: 465 RLRAPFSLQLTSDSGRKLVINNVILANWNAGATYRSLVNY 584
Score = 68.0 bits (142), Expect(2) = 4e-037
Identities = 25/47 (53%), Positives = 35/47 (74%)
Frame = +3 / +3
Query: 30 NYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
N P P HFD+SG A G++AKPG+ DKLR G++ ++++RV CKYP
Sbjct: 144 NLYPGEPCHFDMSGTALGAMAKPGMADKLRAGGVIRMQYKRVPCKYP 284
>gb|AV928430.1|AV928430 AV928430 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basdg18 3', mRNA sequence
Length = 704
Score = 134 bits (287), Expect(2) = 7e-037
Identities = 51/100 (51%), Positives = 74/100 (74%)
Frame = +3 / -2
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDTAK 353
G KI FH+ +G N YLAVL+++ DGD++ +++++ S W M+ SWG+IWRMD+
Sbjct: 643 GLKINFHVVEGSNAVYLAVLIEYEDMDGDVIQVDMKEANSGSWMAMRESWGSIWRMDSNH 464
Query: 354 ALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQF 473
L+GPFS+R+TS+SGKK++A +VIP NWRP+ Y S VQF
Sbjct: 463 RLQGPFSMRITSDSGKKLVANNVIPANWRPNTDYRSFVQF 344
Score = 39.6 bits (80), Expect(2) = 7e-037
Identities = 13/20 (65%), Positives = 17/20 (85%)
Frame = +3 / -2
Query: 111 KLRHCGIMDVEFRRVRCKYP 170
KLRH GI+D++FRRV C +P
Sbjct: 703 KLRHSGIIDIQFRRVPCNFP 644
>gb|CV055940.1|CV055940 BNEL12B5 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL12B5 5' similar to Oryza sativa
beta-expansin, mRNA sequence
Length = 614
Score = 95.5 bits (202), Expect(3) = 1e-036
Identities = 34/66 (51%), Positives = 50/66 (75%)
Frame = +3 / -2
Query: 291 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQ 470
+ +W PMK SWG+IWRMD + GPFS+R+T+ESGK ++A VIP +W+P+ Y+S VQ
Sbjct: 358 TGQWVPMKESWGSIWRMDAHHPMHGPFSLRVTNESGKTLVADQVIPGDWKPNKTYSSLVQ 179
Query: 471 FY*TLN 488
F+* ++
Sbjct: 178 FH*LID 161
Score = 50.1 bits (103), Expect(3) = 1e-036
Identities = 17/30 (56%), Positives = 26/30 (86%)
Frame = +3 / -2
Query: 81 GSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
G++AK G ND+LRH GI++++F+RV C+YP
Sbjct: 598 GAMAKYGRNDELRHAGIINMQFKRVPCQYP 509
Score = 46.0 bits (94), Expect(3) = 1e-036
Identities = 15/37 (40%), Positives = 26/37 (70%)
Frame = +3 / -2
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G + FH+E+G NP Y+A+LV++ DGD+ ++I +
Sbjct: 508 GLTVTFHVEEGSNPFYMAILVEYENGDGDVKQLDIME 398
>gb|BQ459628.1|BQ459628 HA08J01r HA Hordeum vulgare subsp. vulgare cDNA clone HA08J01
5-PRIME, mRNA sequence
Length = 470
Score = 88.6 bits (187), Expect(3) = 7e-036
Identities = 33/69 (47%), Positives = 47/69 (68%)
Frame = +3 / +1
Query: 267 LMEIQDKLSAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPD 446
LME + +W M+ SWG++WR+D+ L+ PFSIR+ +ESGK ++A VIP NWRP+
Sbjct: 211 LMESRGPGGGKWTRMRESWGSVWRLDSNHRLQAPFSIRIRNESGKTLVANKVIPANWRPN 390
Query: 447 AVYTSNVQF 473
Y S VQ+
Sbjct: 391 TFYRSFVQY 417
Score = 55.6 bits (115), Expect(3) = 7e-036
Identities = 22/32 (68%), Positives = 25/32 (78%)
Frame = +3 / +2
Query: 63 LSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVR 158
LSG AFG LAKPGLND+LRH GI+D+E R
Sbjct: 2 LSGTAFGRLAKPGLNDRLRHSGIIDIELHGCR 97
Score = 44.6 bits (91), Expect(3) = 7e-036
Identities = 17/37 (45%), Positives = 26/37 (70%)
Frame = +3 / +1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G KI FH+E+ NP Y AVLV++ DGD+V +++ +
Sbjct: 109 GLKIGFHVEEYSNPVYFAVLVEYEDGDGDVVQVDLME 219
>gb|BE196421.3|BE196421 HVSMEh0092D24f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0092D24f, mRNA sequence
Length = 901
Score = 105 bits (223), Expect(3) = 4e-034
Identities = 38/56 (67%), Positives = 49/56 (87%)
Frame = +3 / +1
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
P TV ITDMNY P+A YHFDLSG AFG++AKPGL++KLRH GI+D++F+RV C++P
Sbjct: 313 PETVVITDMNYYPVAKYHFDLSGTAFGAMAKPGLSEKLRHSGIIDIQFKRVPCEFP 480
Score = 40.5 bits (82), Expect(3) = 4e-034
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = +3 / +3
Query: 300 WKPMKLSWGAIWRMDTAKALKGPFS 374
W PM+ SWG+IW +D L+ PFS
Sbjct: 606 WTPMRESWGSIWGLDFGHRLQAPFS 680
Score = 37.3 bits (75), Expect(3) = 4e-034
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = +3 / +3
Query: 180 KIVFHIEKGCNPNYLAVLVKFVADDGDIV 266
++ FH+E+ NP Y AVL ++ DGD+V
Sbjct: 486 QVTFHVEQXSNPVYFAVLGEYEDGDGDVV 572
>gb|BU980984.1|BU980984 HA22E18r HA Hordeum vulgare subsp. vulgare cDNA clone HA22E18
5-PRIME, mRNA sequence
Length = 567
Score = 80.3 bits (169), Expect(3) = 5e-033
Identities = 31/63 (49%), Positives = 40/63 (63%)
Frame = +3 / +3
Query: 294 AEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQF 473
A W M SWGA+WR ++ L PFS+RLTS SGK ++A +VIP W+P Y S V +
Sbjct: 315 AGWTKMDRSWGAVWRYNSGPMLHAPFSVRLTSSSGKTLVASNVIPAGWKPGGTYRSVVNY 494
Query: 474 Y*T 482
*T
Sbjct: 495 **T 503
Score = 57.4 bits (119), Expect(3) = 5e-033
Identities = 21/40 (52%), Positives = 28/40 (70%)
Frame = +3 / +3
Query: 48 PYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKY 167
P HFDLSG AFG +AKPG D+LR G + +++ RV C +
Sbjct: 66 PVHFDLSGTAFGGMAKPGQADQLRAAGRLQIQYTRVPCNW 185
Score = 41.4 bits (84), Expect(3) = 5e-033
Identities = 14/37 (37%), Positives = 24/37 (64%)
Frame = +3 / +3
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G + F ++ G NP YLAVL+++ DGD+ +E+ +
Sbjct: 189 GMHVAFKVDAGSNPYYLAVLIEYEDGDGDLSSVELME 299
>gb|BM098205.2|BM098205 EBpi03_SQ002_N14_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ002_N14 5', mRNA sequence
Length = 550
Score = 80.3 bits (169), Expect(3) = 5e-033
Identities = 31/63 (49%), Positives = 40/63 (63%)
Frame = +3 / +1
Query: 294 AEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQF 473
A W M SWGA+WR ++ L PFS+RLTS SGK ++A +VIP W+P Y S V +
Sbjct: 301 AGWTKMDRSWGAVWRYNSGPMLHAPFSVRLTSSSGKTLVASNVIPAGWKPGGTYRSVVNY 480
Query: 474 Y*T 482
*T
Sbjct: 481 **T 489
Score = 57.4 bits (119), Expect(3) = 5e-033
Identities = 21/40 (52%), Positives = 28/40 (70%)
Frame = +3 / +1
Query: 48 PYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKY 167
P HFDLSG AFG +AKPG D+LR G + +++ RV C +
Sbjct: 52 PVHFDLSGTAFGGMAKPGQADQLRAAGRLQIQYTRVPCNW 171
Score = 41.4 bits (84), Expect(3) = 5e-033
Identities = 14/37 (37%), Positives = 24/37 (64%)
Frame = +3 / +1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G + F ++ G NP YLAVL+++ DGD+ +E+ +
Sbjct: 175 GMHVAFKVDAGSNPYYLAVLIEYEDGDGDLSSVELME 285
>gb|BJ463021.1|BJ463021 BJ463021 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags25g15 5', mRNA sequence
Length = 532
Score = 92.7 bits (196), Expect(2) = 7e-033
Identities = 32/83 (38%), Positives = 52/83 (62%)
Frame = +3 / +3
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDTAK 353
G I F +++G NP Y L++F DDGD+ + +++ S W M WGA+WR++
Sbjct: 279 GVNIAFRVDQGSNPFYFKTLIEFEDDDGDLKAVALKEAGSGAWTQMTQDWGALWRLNNGN 458
Query: 354 ALKGPFSIRLTSESGKKVIAKDV 422
L+ PFS+RLT +SG+K++ +V
Sbjct: 459 RLRAPFSLRLTXDSGRKLVXNNV 527
Score = 68.0 bits (142), Expect(2) = 7e-033
Identities = 25/47 (53%), Positives = 35/47 (74%)
Frame = +3 / +3
Query: 30 NYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
N P P HFD+SG A G++AKPG+ DKLR G++ ++++RV CKYP
Sbjct: 138 NLYPGEPCHFDMSGTALGAMAKPGMADKLRAGGVIRMQYKRVPCKYP 278
>gb|CV054003.1|CV054003 BNEL105f8 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL105f8 5' similar to Oryza sativa
beta-expansin, mRNA sequence
Length = 702
Score = 99.1 bits (210), Expect(3) = 8e-033
Identities = 36/56 (64%), Positives = 48/56 (85%)
Frame = +3 / +3
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
P TV ITDMNY+P++ YHFDLSG AFG++AK G ND+LRH GI++++F+RV C+YP
Sbjct: 333 PKTVIITDMNYDPVSRYHFDLSGTAFGAMAKYGRNDELRHAGIINMQFKRVPCQYP 500
Score = 46.0 bits (94), Expect(3) = 8e-033
Identities = 15/37 (40%), Positives = 26/37 (70%)
Frame = +3 / +3
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G + FH+E+G NP Y+A+LV++ DGD+ ++I +
Sbjct: 501 GLTVTFHVEEGSNPFYMAILVEYENGDGDVKQLDIME 611
Score = 33.1 bits (66), Expect(3) = 8e-033
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = +3 / +3
Query: 291 SAEWKPMKLSWGAIWRM 341
+ +W PMK SW +IWRM
Sbjct: 651 TGQWVPMKESWXSIWRM 701
>gb|CV063217.1|CV063217 BNEL87h3 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL87h3 5' similar to Oryza sativa
beta-expansin, mRNA sequence
Length = 701
Score = 99.1 bits (210), Expect(3) = 5e-032
Identities = 36/56 (64%), Positives = 48/56 (85%)
Frame = +3 / +3
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
P TV ITDMNY+P++ YHFDLSG AFG++AK G ND+LRH GI++++F+RV C+YP
Sbjct: 339 PKTVIITDMNYDPVSRYHFDLSGTAFGAMAKYGRNDELRHAGIINMQFKRVPCQYP 506
Score = 44.6 bits (91), Expect(3) = 5e-032
Identities = 14/34 (41%), Positives = 25/34 (73%)
Frame = +3 / +3
Query: 183 IVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
+ FH+E+G NP Y+A+LV++ DGD+ ++I +
Sbjct: 516 VTFHVEEGSNPFYMAILVEYENGDGDVKQLDIME 617
Score = 31.8 bits (63), Expect(3) = 5e-032
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +3 / +3
Query: 291 SAEWKPMKLSWGAIW 335
+ +W PMK SWG+IW
Sbjct: 657 TGQWVPMKESWGSIW 701
>gb|BQ753555.1|BQ753555 EBan01_SQ004_J12_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_J12 5', mRNA sequence
Length = 286
Score = 137 bits (293), Expect = 9e-032
Identities = 50/93 (53%), Positives = 71/93 (76%)
Frame = +3 / +1
Query: 126 GIMDVEFRRVRCKYPAGQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWK 305
G ++++FRRV+CKYP G K+ FH+EKG +PNYLA++VKF+ DGD+V ++I+ K +W
Sbjct: 4 GEVEIKFRRVKCKYPEGTKVNFHVEKGSSPNYLALVVKFLQGDGDVVAVDIKPKGKDKWI 183
Query: 306 PMKLSWGAIWRMDTAKALKGPFSIRLTSESGKK 404
+K SWGA+WR+DT L GPFS+R T+E G K
Sbjct: 184 ELKESWGAVWRIDTPDKLIGPFSVRYTTEGGTK 282
>gb|BQ459308.1|BQ459308 HA01I15r HA Hordeum vulgare subsp. vulgare cDNA clone HA01I15
5-PRIME, mRNA sequence
Length = 396
Score = 86.8 bits (183), Expect(3) = 3e-031
Identities = 30/57 (52%), Positives = 43/57 (75%)
Frame = +3 / +2
Query: 291 SAEWKPMKLSWGAIWRMDTAKALKGPFSIRLTSESGKKVIAKDVIPPNWRPDAVYTS 461
+ EW PMK SWG+IWRMD ++GPF +R+T+ESGK ++A VIP +W+P+ Y+S
Sbjct: 224 TGEWVPMKESWGSIWRMDAHHPMRGPFWLRVTNESGKTLVADKVIPGDWKPNKTYSS 394
Score = 45.5 bits (93), Expect(3) = 3e-031
Identities = 15/37 (40%), Positives = 26/37 (70%)
Frame = +3 / +2
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G + FH+E+G NP Y+A+LV++ DGD+ ++I +
Sbjct: 74 GLTVTFHVEEGSNPFYMAILVEYDNGDGDVKQLDIME 184
Score = 40.9 bits (83), Expect(3) = 3e-031
Identities = 13/24 (54%), Positives = 20/24 (83%)
Frame = +3 / +2
Query: 99 GLNDKLRHCGIMDVEFRRVRCKYP 170
G D+LRH GI++++F+RV C+YP
Sbjct: 2 GTRDELRHAGIINMQFKRVPCQYP 73
>gb|CA019997.1|CA019997 HV14A15r HV Hordeum vulgare subsp. vulgare cDNA clone HV14A15
5-PRIME, mRNA sequence
Length = 616
Score = 105 bits (225), Expect(2) = 5e-030
Identities = 39/54 (72%), Positives = 46/54 (85%)
Frame = +3 / +1
Query: 9 TVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
TV ITDMNY P+APYHFDLSG AFG LAKPG ND+LRH GI+D++F RV C++P
Sbjct: 280 TVMITDMNYYPVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFP 441
Score = 45.1 bits (92), Expect(2) = 5e-030
Identities = 15/37 (40%), Positives = 27/37 (72%)
Frame = +3 / +1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G K+ FH+E+G N Y+A+LV++ DGD+V +++ +
Sbjct: 442 GLKVGFHVEEGSNAVYMAILVEYENGDGDVVQVDLME 552
>gb|BI958784.1|BI958784 HVSMEn0016J13f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0016J13f, mRNA sequence
Length = 656
Score = 105 bits (223), Expect(2) = 7e-030
Identities = 38/56 (67%), Positives = 49/56 (87%)
Frame = +3 / +3
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
P TV ITDMNY P+A YHFDLSG AFG++AKPGL++KLRH GI+D++F+RV C++P
Sbjct: 387 PETVVITDMNYYPVAKYHFDLSGTAFGAMAKPGLSEKLRHSGIIDIQFKRVPCEFP 554
Score = 45.5 bits (93), Expect(2) = 7e-030
Identities = 17/31 (54%), Positives = 22/31 (70%)
Frame = +3 / +3
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIV 266
G K+ FH+E+G NP Y AVLV++ DGD V
Sbjct: 555 GLKVTFHVEQGSNPVYFAVLVEYEDGDGDXV 647
>gb|BQ469137.1|BQ469137 HM03I08r HM Hordeum vulgare subsp. vulgare cDNA clone HM03I08
5-PRIME, mRNA sequence
Length = 616
Score = 130 bits (279), Expect = 7e-030
Identities = 49/77 (63%), Positives = 60/77 (77%)
Frame = +3 / +3
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYPAGQK 182
P+TV+ITD NYEPIAPYH DLSGKAFG++A G LR G ++++FRRVRCKY G K
Sbjct: 384 PITVFITDKNYEPIAPYHIDLSGKAFGAMATAGNEQTLRSFGELELQFRRVRCKYAPGTK 563
Query: 183 IVFHIEKGCNPNYLAVL 233
+ FH+E G NPNYLA+L
Sbjct: 564 VTFHVETGSNPNYLAIL 614
>gb|BU995044.1|BU995044 HM09A07r HM Hordeum vulgare subsp. vulgare cDNA clone HM09A07
5-PRIME, mRNA sequence
Length = 628
Score = 104 bits (222), Expect(2) = 1e-029
Identities = 41/71 (57%), Positives = 50/71 (70%)
Frame = +3 / +1
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYPAGQK 182
P V ITD N EPIA YHFDLSG AFG++AK G KLR G ++++FRRV+CKYP G K
Sbjct: 346 PTIVTITDKNEEPIAAYHFDLSGHAFGTMAKKGQEQKLRDAGEVEIKFRRVKCKYPEGTK 525
Query: 183 IVFHIEKGCNP 215
+ FH +G P
Sbjct: 526 VNFHXGEGLQP 558
Score = 45.1 bits (92), Expect(2) = 1e-029
Identities = 16/28 (57%), Positives = 24/28 (85%)
Frame = +3 / +2
Query: 198 EKGCNPNYLAVLVKFVADDGDIVLMEIQ 281
EKG +PNYLA++VKF+ DGD+V ++I+
Sbjct: 542 EKGSSPNYLALVVKFLQGDGDVVAVDIK 625
>gb|BI957603.1|BI957603 HVSMEn0010F11f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0010F11f, mRNA sequence
Length = 630
Score = 105 bits (223), Expect(2) = 2e-029
Identities = 38/56 (67%), Positives = 49/56 (87%)
Frame = +3 / +1
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
P TV ITDMNY P+A YHFDLSG AFG++AKPGL++KLRH GI+D++F+RV C++P
Sbjct: 349 PETVVITDMNYYPVAKYHFDLSGTAFGAMAKPGLSEKLRHSGIIDIQFKRVPCEFP 516
Score = 43.7 bits (89), Expect(2) = 2e-029
Identities = 15/37 (40%), Positives = 26/37 (70%)
Frame = +3 / +1
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G K+ FH+E+G NP Y VLV++ DG++V +++ +
Sbjct: 517 GLKVTFHVEQGSNPVYFPVLVEY*DGDGNLVQVDLME 627
>gb|BE230894.2|BE230894 HVSMEg0001I02f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0001I02f, mRNA sequence
Length = 606
Score = 127 bits (273), Expect = 5e-029
Identities = 50/81 (61%), Positives = 63/81 (77%)
Frame = +3 / +1
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYPAGQK 182
P V ITD N EPIA YHFDLSG AFG++AK G KLR G ++++FRRV+CKYP G K
Sbjct: 325 PTIVTITDKNEEPIAAYHFDLSGHAFGTMAKEGQ*QKLRDAGEVEIKFRRVKCKYPEGTK 504
Query: 183 IVFHIEKGCNPNYLAVLVKFV 245
+ FH+EKG +PNYLA++VKF+
Sbjct: 505 VNFHVEKGSSPNYLALVVKFL 567
>gb|BQ467895.1|BQ467895 HR01E04r HR Hordeum vulgare subsp. vulgare cDNA clone HR01E04
5-PRIME, mRNA sequence
Length = 540
Score = 127 bits (271), Expect = 9e-029
Identities = 47/94 (50%), Positives = 70/94 (74%)
Frame = +3 / +2
Query: 192 HIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDTAKALKGPF 371
H+ +G N YLAVL+++ DGD++ +++++ S W M+ SWG+IWRMD+ L+GPF
Sbjct: 2 HVVEGSNAVYLAVLIEYEDMDGDVIQVDMKEANSGSWMAMRESWGSIWRMDSNHRLQGPF 181
Query: 372 SIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQF 473
S+R+TS+SGKK++A +VIP NWRP+ Y S VQF
Sbjct: 182 SMRITSDSGKKLVANNVIPANWRPNTDYRSFVQF 283
>gb|BI959353.1|BI959353 HVSMEn0019F12f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0019F12f, mRNA sequence
Length = 669
Score = 105 bits (223), Expect(2) = 2e-028
Identities = 38/56 (67%), Positives = 49/56 (87%)
Frame = +3 / +2
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
P TV ITDMNY P+A YHFDLSG AFG++AKPGL++KLRH GI+D++F+RV C++P
Sbjct: 401 PETVVITDMNYYPVAKYHFDLSGTAFGAMAKPGLSEKLRHSGIIDIQFKRVPCEFP 568
Score = 40.9 bits (83), Expect(2) = 2e-028
Identities = 14/32 (43%), Positives = 23/32 (71%)
Frame = +3 / +1
Query: 180 KIVFHIEKGCNPNYLAVLVKFVADDGDIVLME 275
++ FH+E+G NP Y AVLV++ DG +V ++
Sbjct: 574 QVTFHVEQGSNPVYFAVLVEYQDGDGHVVQVD 669
>gb|CB880652.1|CB880652 HM07E17w HM Hordeum vulgare subsp. vulgare cDNA clone HM07E17
3-PRIME, mRNA sequence
Length = 562
Score = 126 bits (269), Expect = 2e-028
Identities = 46/94 (48%), Positives = 66/94 (70%)
Frame = +3 / -2
Query: 192 HIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDTAKALKGPF 371
H+E+G NP Y AVLV++ DGD+V +++ + S W PM+ SWG+IWR+D+ L+ PF
Sbjct: 561 HVEQGSNPVYFAVLVEYEDGDGDVVQVDLMEANSGTWTPMRESWGSIWRLDSGHRLQAPF 382
Query: 372 SIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQF 473
S+R+T+ESGK ++A VIP NW P Y S VQ+
Sbjct: 381 SMRITNESGKTLVADKVIPANWAPSTFYRSIVQY 280
>gb|CB881234.1|CB881234 HM09C23w HM Hordeum vulgare subsp. vulgare cDNA clone HM09C23
3-PRIME, mRNA sequence
Length = 563
Score = 126 bits (269), Expect = 2e-028
Identities = 46/94 (48%), Positives = 66/94 (70%)
Frame = +3 / -2
Query: 192 HIEKGCNPNYLAVLVKFVADDGDIVLMEIQDKLSAEWKPMKLSWGAIWRMDTAKALKGPF 371
H+E+G NP Y AVLV++ DGD+V +++ + S W PM+ SWG+IWR+D+ L+ PF
Sbjct: 562 HVEQGSNPVYFAVLVEYEDGDGDVVQVDLMEANSGTWTPMRESWGSIWRLDSGHRLQAPF 383
Query: 372 SIRLTSESGKKVIAKDVIPPNWRPDAVYTSNVQF 473
S+R+T+ESGK ++A VIP NW P Y S VQ+
Sbjct: 382 SMRITNESGKTLVADKVIPANWAPSTFYRSIVQY 281
>gb|AV917082.1|AV917082 AV917082 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20l09 5', mRNA sequence
Length = 323
Score = 100 bits (213), Expect(2) = 2e-028
Identities = 36/50 (72%), Positives = 43/50 (86%)
Frame = +3 / +2
Query: 21 TDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
TDMNY P+APYHFDLSG AFG LAKPG ND+LRH GI+D++F RV C++P
Sbjct: 2 TDMNYYPVAPYHFDLSGTAFGKLAKPGRNDELRHAGIIDIQFTRVACEFP 151
Score = 45.1 bits (92), Expect(2) = 2e-028
Identities = 15/37 (40%), Positives = 27/37 (72%)
Frame = +3 / +2
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G K+ FH+E+G N Y+A+LV++ DGD+V +++ +
Sbjct: 152 GLKVGFHVEEGSNAVYMAILVEYENGDGDVVQVDLME 262
>gb|CV063939.1|CV063939 BNEL94h5 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL94h5 5' similar to Oryza sativa
beta-expansin, mRNA sequence
Length = 661
Score = 99.1 bits (210), Expect(2) = 3e-028
Identities = 36/56 (64%), Positives = 48/56 (85%)
Frame = +3 / +2
Query: 3 PVTVYITDMNYEPIAPYHFDLSGKAFGSLAKPGLNDKLRHCGIMDVEFRRVRCKYP 170
P TV ITDMNY+P++ YHFDLSG AFG++AK G ND+LRH GI++++F+RV C+YP
Sbjct: 332 PKTVIITDMNYDPVSRYHFDLSGTAFGAMAKYGRNDELRHAGIINMQFKRVPCQYP 499
Score = 46.0 bits (94), Expect(2) = 3e-028
Identities = 15/37 (40%), Positives = 26/37 (70%)
Frame = +3 / +2
Query: 174 GQKIVFHIEKGCNPNYLAVLVKFVADDGDIVLMEIQD 284
G + FH+E+G NP Y+A+LV++ DGD+ ++I +
Sbjct: 500 GLTVTFHVEEGSNPFYMAILVEYENGDGDVKQLDIME 610
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 392,224,455
Number of Sequences: 312970
Number of extensions: 8003197
Number of successful extensions: 146032
Number of sequences better than 1.0e-020: 125
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8356091673
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)