TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1716323.2.2
(764 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU986484.1|BU986484 HF12A02r HF Hordeum vulgare subsp. v... 320 6e-087
gb|BF064865.2|BF064865 HV_CEb0021D12f Hordeum vulgare seedl... 297 5e-080
gb|BG418054.1|BG418054 HVSMEk0021B11f Hordeum vulgare testa... 284 3e-076
gb|BG414540.1|BG414540 HVSMEk0002N03f Hordeum vulgare testa... 203 5e-062
gb|AV916926.1|AV916926 AV916926 K. Sato unpublished cDNA li... 169 8e-057
gb|CK124865.1|CK124865 BES1824110k15 BES1824 Hordeum vulgar... 215 3e-055
gb|BE454671.3|BE454671 HVSMEh0094P16f Hordeum vulgare 5-45 ... 212 2e-054
gb|CA007491.1|CA007491 HU08C03r HU Hordeum vulgare subsp. v... 202 2e-051
gb|BQ763608.1|BQ763608 EBro02_SQ008_M23_R root, 3 week, hyd... 200 8e-051
gb|AV918175.1|AV918175 AV918175 K. Sato unpublished cDNA li... 146 3e-050
gb|BE213865.1|BE213865 HV_CEb0001H21f Hordeum vulgare seedl... 198 4e-050
gb|BF623201.2|BF623201 HVSMEa0015K22f Hordeum vulgare seedl... 179 7e-046
gb|BQ766207.1|BQ766207 EBro08_SQ005_H07_R root, 3 week, dro... 174 4e-043
gb|AL510457.1|AL510457 AL510457 Hordeum vulgare Barke devel... 173 1e-042
gb|CB882386.1|CB882386 HL01L15w HL Hordeum vulgare subsp. v... 171 4e-042
gb|AL507037.1|AL507037 AL507037 Hordeum vulgare Barke devel... 145 1e-038
gb|BI956935.1|BI956935 HVSMEn0006D04f Hordeum vulgare rachi... 147 6e-035
gb|AL502418.1|AL502418 AL502418 Hordeum vulgare Barke roots... 119 1e-032
gb|CV059902.1|CV059902 BNEL52b8 Barley EST endosperm librar... 137 6e-032
gb|CV061694.1|CV061694 BNEL70h10 Barley EST endosperm libra... 137 6e-032
gb|BI959839.1|BI959839 HVSMEn0021P10f Hordeum vulgare rachi... 98 7e-030
gb|CA005904.1|CA005904 HU08C03u HU Hordeum vulgare subsp. v... 127 5e-029
gb|CA016130.1|CA016130 HV10D20u HV Hordeum vulgare subsp. v... 127 1e-028
gb|AL510866.1|AL510866 AL510866 Hordeum vulgare Barke devel... 121 6e-027
gb|BQ465081.1|BQ465081 HU02J12r HU Hordeum vulgare subsp. v... 117 6e-026
gb|AV833853.1|AV833853 AV833853 K. Sato unpublished cDNA li... 90 7e-025
gb|BG344702.1|BG344702 HVSMEg0015E07f Hordeum vulgare pre-a... 112 2e-024
gb|BQ757736.1|BQ757736 EBem10_SQ005_B05_R embryo, 2 Day ger... 100 7e-021
gb|BQ766052.1|BQ766052 EBro03_SQ008_L23_R root, 3 week, wat... 91 9e-021
>gb|BU986484.1|BU986484 HF12A02r HF Hordeum vulgare subsp. vulgare cDNA clone HF12A02
5-PRIME, mRNA sequence
Length = 577
Score = 320 bits (693), Expect = 6e-087
Identities = 133/156 (85%), Positives = 144/156 (92%)
Frame = +3 / +1
Query: 102 GPVVMKGYVGDDEATAATVDSEGWLKTGDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAE 281
GP+VMKGYVGDD+ATA TVDSEGWLKTGDLCYFNEDG LYIVDRLKELIKYKGYQVPPAE
Sbjct: 10 GPLVMKGYVGDDKATAETVDSEGWLKTGDLCYFNEDGFLYIVDRLKELIKYKGYQVPPAE 189
Query: 282 LEHILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVR 461
LEHIL SHP+I DAAVI YPDED GQLPMAFIVR+PGS+LT +QVMD+VAK VAPYKKVR
Sbjct: 190 LEHILQSHPEIADAAVIGYPDEDVGQLPMAFIVRQPGSSLTGKQVMDYVAKHVAPYKKVR 369
Query: 462 RVAFVSAIPKSPAGKILRRELVEXAVSMGTXKL*SL 569
RVAFV+AIPKSPAGKILRRELV+ A+SMG KL* +
Sbjct: 370 RVAFVAAIPKSPAGKILRRELVQQAMSMGASKL*DV 477
Score = 252 bits (544), Expect = 2e-066
Identities = 98/157 (62%), Positives = 119/157 (75%)
Frame = -1 / -2
Query: 563 SKLXGTHGHGXLYKLPPQDLPSRRFGNCRNKGHSTNFLVGCDLFSHEIHDLLLGKV*PWF 384
SKL GTHGHG L+KLP QDLP RRFG CR++GHS +FLVG ++FSH IHDL K * W
Sbjct: 471 SKLGGTHGHGLLHKLPSQDLPRRRFGYCRDEGHSADFLVGRNMFSHVIHDLFPSKA*TWL 292
Query: 383 PHYKSHWELPGVFVWIRNNCCVHDIRMRIQDMLQFGRRYLISLVLYQLFQPINNVE*PIL 204
PHYK HW+LP +F+WI +NC + + RM + +MLQF R+L+S VLYQL Q INNVE I
Sbjct: 291 PHYKGHWQLPNIFIWISDNCSISNFRM*L*NMLQFSWRHLVSFVLYQLLQTINNVEKSIF 112
Query: 203 VEVA*VTGFQPAF*IHCCCSSLVVTDITFHDDWSSDP 93
+EVA +T FQPAF*+HC S VVTDITFHD+W+ P
Sbjct: 111 IEVAKITSFQPAF*VHCFRSCFVVTDITFHDEWALVP 1
Score = 221 bits (476), Expect = 5e-057
Identities = 103/154 (66%), Positives = 107/154 (69%)
Frame = -2 / -3
Query: 562 QSLXVPMDTACSTSSRRKIFPAGDLGIAETKATRRTFL*GATCLATKSMTCCSVRFDPGF 383
QSL PMD AC TSSRRKIFPAGDLGIA TKATRRTFL*GATCLAT SMTC VR +PG
Sbjct: 470 QSLEAPMDMACCTSSRRKIFPAGDLGIAATKATRRTFL*GATCLAT*SMTCFPVRLEPGC 291
Query: 382 LTIKAIGSCPXXXXXXXXXXXXXXXG*EFRICSSSAGGT*YPLYFINSFNRSTM*SNPSS 203
LTIKAIGSCP G + RICSSSAGGT YPLYFINSF RSTM* NPSS
Sbjct: 290 LTIKAIGSCPTSSSGYPITAASAISGCDCRICSSSAGGTWYPLYFINSFKRSTM*RNPSS 111
Query: 202 LK*HRSPVFNQXXXXXXXXXXXXXXXXPFMTTGP 101
LK* RSPVF+Q PFMT+GP
Sbjct: 110 LK*QRSPVFSQPSESTVSAVALSSPT*PFMTSGP 9
Score = 149 bits (320), Expect = 2e-035
Identities = 72/125 (57%), Positives = 82/125 (65%)
Frame = +1 / +2
Query: 118 KVMSVTTRLLQQQWIQKAG*KPVTYATSTRMGYSTLLIG*KS**STRDIRYLLPNWXXXX 297
KVMSVTT+ L++QW QKAG*K V +ATS +M +STLLI *+S**ST+D R L NW
Sbjct: 26 KVMSVTTKQLRKQWTQKAG*KLVIFATSMKMDFSTLLIV*RS**STKDTRCLQLNWSIFY 205
Query: 298 XXXXXXWTQQLFLIQTKTPGNSQWLL**GNQGQTLPSNKSWISWLNRSHPTRKFVEWPLF 477
QL IQ K GN QW L**GNQ Q L NKSWI+WLN PTRK EWP
Sbjct: 206 NHILKLLMLQLSDIQMKMLGNCQWPL**GNQVQALLGNKSWITWLNMLRPTRKSAEWPSS 385
Query: 478 LQFPN 492
Q+PN
Sbjct: 386 RQYPN 400
Score = 100 bits (214), Expect(2) = 5e-043
Identities = 46/70 (65%), Positives = 49/70 (70%)
Frame = +2 / +3
Query: 356 ATPNGFYSEETRVKPYRATSHGFRG*TGRTLQESSSSGLCFCNSQIACWEDLAAGACRAX 535
AT NG YSE TR KPY TSHG RG*T LQES SGL N+QI+C EDLA GAC A
Sbjct: 264 ATANGLYSEATRFKPYWETSHGLRG*TCCALQESPPSGLRRGNTQISCGEDLATGACAAS 443
Query: 536 RVHGYLQALK 565
VHG LQAL+
Sbjct: 444 HVHGCLQALR 473
Score = 93.6 bits (198), Expect(2) = 5e-043
Identities = 41/62 (66%), Positives = 46/62 (74%)
Frame = +2 / +3
Query: 107 SRHERLCR*RRGYCSNSGFRRLVENR*PMLLQRGWVTLHC*SVERVDKVQGISGTSCRTG 286
+RHERLCR*R+ C NSG RRL EN * +LLQ W++LHC*S E VDKVQ I G S TG
Sbjct: 15 TRHERLCR*RQSNCGNSGLRRLAENW*SLLLQ*RWISLHC*SFEGVDKVQRIPGASS*TG 194
Query: 287 AY 292
AY
Sbjct: 195 AY 200
Score = 60.6 bits (126), Expect(3) = 2e-023
Identities = 28/48 (58%), Positives = 31/48 (64%)
Frame = -3 / -1
Query: 312 YQDENSGYAPVRQEVPDIPCTLSTLSTDQQCRVTHPR*SSIGHRFSTS 169
+QD YAPV+ E P I CTLST S DQQCR H *SS H+FS S
Sbjct: 220 FQDVIVEYAPVQLEAPGILCTLSTPSNDQQCREIHLH*SSKDHQFSAS 77
Score = 58.8 bits (122), Expect(3) = 2e-023
Identities = 24/40 (60%), Positives = 28/40 (70%)
Frame = -3 / -1
Query: 567 NFKAXRYPWTRXALQAPAARSSQQAIWELQKQRPLDELSC 448
+ KA R+PWT A QAP ARSS Q IW L ++RPL LSC
Sbjct: 475 HLKAWRHPWTWLAAQAPVARSSPQEIWVLPRRRPLGGLSC 356
Score = 26.7 bits (52), Expect(3) = 2e-023
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = -3 / -1
Query: 408 AR*GLTLVSSL*KPLGVA 355
++*GL LV+SL*+PL VA
Sbjct: 316 SQ*GLNLVASL*RPLAVA 263
>gb|BF064865.2|BF064865 HV_CEb0021D12f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0021D12f, mRNA sequence
Length = 806
Score = 297 bits (643), Expect = 5e-080
Identities = 118/180 (65%), Positives = 148/180 (82%)
Frame = +3 / +3
Query: 3 SVGKLASHLEAKIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATVDSEGWLKT 182
+ G L+ + EAKIVDP TGE L + GELW+RGP VMKGY + EAT +TV +GWLKT
Sbjct: 78 TAGLLSPNTEAKIVDPETGEALPVNRTGELWIRGPYVMKGYFKNTEATQSTVTPDGWLKT 257
Query: 183 GDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQL 362
GDLCY +EDG L++VDRLKELIKYKGYQVPPAELE +L +HP++ D AVIP+PD + GQ
Sbjct: 258 GDLCYIDEDGYLFVVDRLKELIKYKGYQVPPAELEALLLTHPEVSDVAVIPFPDREVGQF 437
Query: 363 PMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSPAGKILRRELVEXAVS 542
PMA++VRK GSNL+ Q+VM+FVAKQVAPYKKVR+VAFV+ IPK+ +GKILR++L++ A S
Sbjct: 438 PMAYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVAFVTDIPKNASGKILRKDLIKLATS 617
Score = 133 bits (284), Expect = 2e-030
Identities = 74/180 (41%), Positives = 100/180 (55%)
Frame = -1 / -1
Query: 542 GHGXLYKLPPQDLPSRRFGNCRNKGHSTNFLVGCDLFSHEIHDLLLGKV*PWFPHYKSHW 363
G L K+ PQ L R G+ ++ H + LV C LF H++H LL ++ P+ PH H
Sbjct: 617 GGRELDKILPQYLARRILGDVCHERHLPHLLVRCYLFRHKLHHLLR*QIAPFLPHDIGHR 438
Query: 362 ELPGVFVWIRNNCCVHDIRMRIQDMLQFGRRYLISLVLYQLFQPINNVE*PILVEVA*VT 183
EL + VW RNN + ++ M Q Q R+LI+ V QLFQ +++ E PILV VA*VT
Sbjct: 437 ELTDLPVWERNNSDIGNLWMGQQKSFQLCWRHLIAFVFDQLFQTVHHEEIPILVYVA*VT 258
Query: 182 GFQPAF*IHCCCSSLVVTDITFHDDWSSDPELAALPWPKHLAGGGIDDLCLQVRCQLPDR 3
G +P+ H L V +++FHD S DPELA + LA +DDL L V Q P R
Sbjct: 257 GLEPSVGRHRRLCCLCVLEVSFHDVGSPDPELAGAVHRQRLACLRVDDLGLGVGRQEPGR 78
Score = 104 bits (222), Expect(2) = 4e-027
Identities = 58/124 (46%), Positives = 68/124 (54%)
Frame = -2 / -2
Query: 544 MDTACSTSSRRKIFPAGDLGIAETKATRRTFL*GATCLATKSMTCCSVRFDPGFLTIKAI 365
++ A S I P LG++ T AT TFL*GATC AT S+T C+ + P FLT AI
Sbjct: 619 LEVASLIRSFLSILPDAFLGMSVTNATFLTFL*GATCFATNSITSCADKLLPFFLTT*AI 440
Query: 364 GSCPXXXXXXXXXXXXXXXG*EFRICSSSAGGT*YPLYFINSFNRSTM*SNPSSLK*HRS 185
G+ P G R S+SAGGT* PLY INSF RST PSS *HRS
Sbjct: 439 GN*PTSLSGNGITATSETSGWVSRRASNSAGGT**PLYLINSFRRSTTKRYPSSSM*HRS 260
Query: 184 PVFN 173
PV +
Sbjct: 259 PVLS 248
Score = 36.8 bits (74), Expect(2) = 4e-027
Identities = 19/30 (63%), Positives = 19/30 (63%)
Frame = -2 / -2
Query: 118 FMTTGPRTQSSPRCPGPSTSPVEGSTIFAS 29
FMT GP QSSP S SPV GSTI AS
Sbjct: 193 FMT*GPLIQSSPVRFTGSASPVSGSTILAS 104
>gb|BG418054.1|BG418054 HVSMEk0021B11f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0021B11f, mRNA sequence
Length = 491
Score = 284 bits (615), Expect = 3e-076
Identities = 116/134 (86%), Positives = 124/134 (92%)
Frame = +3 / +1
Query: 3 SVGKLASHLEAKIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATVDSEGWLKT 182
SVGKLASHL+AKIVDPSTGE L PGQRGEL ++GP+VMKGYVGDD+ATA TVDSEGWLKT
Sbjct: 82 SVGKLASHLQAKIVDPSTGEALWPGQRGELLIKGPLVMKGYVGDDKATAETVDSEGWLKT 261
Query: 183 GDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQL 362
GDLCYFNEDG LYIVDRLKELIKYKGYQ PPAELEHIL SHP+I DAAVI YPDED GQL
Sbjct: 262 GDLCYFNEDGFLYIVDRLKELIKYKGYQGPPAELEHILQSHPEIADAAVIGYPDEDVGQL 441
Query: 363 PMAFIVRKPGSNLT 404
PMAFIVR+PGS+LT
Sbjct: 442 PMAFIVRQPGSSLT 483
Score = 188 bits (406), Expect = 2e-047
Identities = 79/132 (59%), Positives = 95/132 (71%)
Frame = -1 / -3
Query: 401 KV*PWFPHYKSHWELPGVFVWIRNNCCVHDIRMRIQDMLQFGRRYLISLVLYQLFQPINN 222
K * W PHYK HW+LP +F+WI NC + + RM + +MLQF R L+S VLYQL Q INN
Sbjct: 480 KA*TWLPHYKGHWQLPNIFIWISYNCSISNFRM*L*NMLQFSWRPLVSFVLYQLLQTINN 301
Query: 221 VE*PILVEVA*VTGFQPAF*IHCCCSSLVVTDITFHDDWSSDPELAALPWPKHLAGGGID 42
VE I +EVA +T F+PAF*+HC S VVT+ITFHD+W+ D ELAAL P+ LAGG ID
Sbjct: 300 VEKSIFIEVAKITSFKPAF*VHCFRSCFVVTNITFHDEWALDQELAALARPERLAGGRID 121
Query: 41 DLCLQVRCQLPD 6
L LQVR QL D
Sbjct: 120 YLRLQVRRQLAD 85
Score = 174 bits (375), Expect = 4e-043
Identities = 81/137 (59%), Positives = 87/137 (63%)
Frame = -2 / -1
Query: 412 CCSVRFDPGFLTIKAIGSCPXXXXXXXXXXXXXXXG*EFRICSSSAGGT*YPLYFINSFN 233
C VR +PG LTIKAIGSCP G + RICSSSAGG YPLYFINSF
Sbjct: 491 CFPVRLEPGCLTIKAIGSCPTSSSGYPITAASAISGCDCRICSSSAGGPWYPLYFINSFK 312
Query: 232 RSTM*SNPSSLK*HRSPVFNQXXXXXXXXXXXXXXXXPFMTTGPRTQSSPRCPGPSTSPV 53
RSTM* NPSSLK* RSPV +Q PFMT+GP +SSPR PG S SPV
Sbjct: 311 RSTM*RNPSSLK*QRSPVLSQPSESTVSAVALSSPT*PFMTSGPLIKSSPRWPGQSASPV 132
Query: 52 EGSTIFASKCDASFPTE 2
+GSTIFA KCDAS PTE
Sbjct: 131 DGSTIFACKCDASLPTE 81
Score = 105 bits (223), Expect(2) = 6e-026
Identities = 49/95 (51%), Positives = 58/95 (61%)
Frame = +2 / +3
Query: 8 REAGIALGGKDRRSLHXXXXXXXXXXXXLGPRTSRHERLCR*RRGYCSNSGFRRLVENR* 187
R+AG+AL +D RS+H L +RHERLC *R+ C NSG RRL N *
Sbjct: 87 RQAGVALASEDSRSVHRRGALAGPARRALDQGPTRHERLCW*RQSNCGNSGLRRLA*NW* 266
Query: 188 PMLLQRGWVTLHC*SVERVDKVQGISGTSCRTGAY 292
+LLQ W++LHC*S E VDKVQ I G S TGAY
Sbjct: 267 SLLLQ*RWISLHC*SFEGVDKVQRIPGASS*TGAY 371
Score = 96.8 bits (205), Expect(2) = 6e-023
Identities = 53/98 (54%), Positives = 61/98 (62%)
Frame = +1 / +2
Query: 118 KVMSVTTRLLQQQWIQKAG*KPVTYATSTRMGYSTLLIG*KS**STRDIRYLLPNWXXXX 297
KVM VTT+ L++QW QKAG K V +ATS +M +STLLI *+S**ST+D R L NW
Sbjct: 197 KVMLVTTKQLRKQWTQKAGLKLVIFATSMKMDFSTLLIV*RS**STKDTRGLQLNWSIFY 376
Query: 298 XXXXXXWTQQLFLIQTKTPGNSQWLL**GNQGQTLPSN 411
QL IQ K GN QW L**GNQ Q L N
Sbjct: 377 NHILKLLMLQL*DIQMKMLGNCQWPL**GNQVQALLGN 490
Score = 32.2 bits (64), Expect(2) = 6e-026
Identities = 13/19 (68%), Positives = 13/19 (68%)
Frame = +2 / +3
Query: 356 ATPNGFYSEETRVKPYRAT 412
AT NG YSE TR KPY T
Sbjct: 435 ATANGLYSEATRFKPYWET 491
Score = 30.4 bits (60), Expect(2) = 6e-023
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +1 / +2
Query: 7 SGSWHRTWRQRSSIPPPAR 63
S SW RT ++R SI PPAR
Sbjct: 86 SASWRRTCKRR*SIRPPAR 142
>gb|BG414540.1|BG414540 HVSMEk0002N03f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0002N03f, mRNA sequence
Length = 876
Score = 203 bits (437), Expect(2) = 5e-062
Identities = 78/121 (64%), Positives = 103/121 (85%)
Frame = +3 / +3
Query: 165 EGWLKTGDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPD 344
+GWL TGDL YF+E+G L++VDRLKELIKYKG+Q+ PAELE +L SHP+I+DA VIP+PD
Sbjct: 294 QGWLHTGDLGYFDEEGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPD 473
Query: 345 EDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSPAGKILRREL 524
++AG++P+A++VR P S+LTE V F+AKQV YK++RRV FV ++PKS AGKILRREL
Sbjct: 474 DEAGEVPIAYVVRSPASSLTEVDVQKFIAKQVTYYKRLRRVTFVESVPKSAAGKILRREL 653
Query: 525 V 527
+
Sbjct: 654 I 656
Score = 90.4 bits (191), Expect(2) = 3e-021
Identities = 55/123 (44%), Positives = 61/123 (49%)
Frame = -2 / -3
Query: 541 DTACSTSSRRKIFPAGDLGIAETKATRRTFL*GATCLATKSMTCCSVRFDPGFLTIKAIG 362
D C+ SS KI PA D G TK TR + L* TCLA T SV G LT AIG
Sbjct: 670 DLTCAMSSLLKILPAADFGTLSTKVTRLSLL*YVTCLAINFWTSTSVNELAGDLTT*AIG 491
Query: 361 SCPXXXXXXXXXXXXXXXG*EFRICSSSAGGT*YPLYFINSFNRSTM*SNPSSLK*HRSP 182
+ P G*+ SSSAG PLY I+SF+ ST S PSS K RSP
Sbjct: 490 TSPASSSGNGITTASRISG*DRSNPSSSAGAIWKPLYLISSFSLSTTKSWPSSSKYPRSP 311
Query: 181 VFN 173
V N
Sbjct: 310 VCN 302
Score = 55.1 bits (114), Expect(2) = 5e-062
Identities = 23/52 (44%), Positives = 33/52 (63%)
Frame = +3 / +3
Query: 3 SVGKLASHLEAKIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATV 158
S G L +EAK++D T + L P Q GE+ +RGP +M+GY + EAT T+
Sbjct: 135 STGTLVVGVEAKVIDVETQKHLPPSQLGEICIRGPHIMQGYFNNVEATEFTI 290
Score = 31.3 bits (62), Expect(2) = 3e-021
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -1 / -2
Query: 155 CCCSSLVVTDITFHDDWSSDPELAALPW 72
C SSL + +I H WSSD + + L W
Sbjct: 287 CKFSSLYIVEIPLHYVWSSDTDFS*LTW 204
>gb|AV916926.1|AV916926 AV916926 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20b24 5', mRNA sequence
Length = 547
Score = 169 bits (363), Expect(3) = 8e-057
Identities = 64/101 (63%), Positives = 86/101 (85%)
Frame = +3 / +2
Query: 165 EGWLKTGDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPD 344
+GWL TGDL YF+E+G L++VDRLKELIKYKG+Q+ PAELE +L SHP+I+DA VIP+PD
Sbjct: 176 QGWLHTGDLGYFDEEGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPD 355
Query: 345 EDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRV 467
++AG++P+A++VR P S+LTE V F+AKQV YK++RRV
Sbjct: 356 DEAGEVPIAYVVRSPASSLTEVDVQKFIAKQVTYYKRLRRV 478
Score = 53.3 bits (110), Expect(3) = 8e-057
Identities = 22/50 (44%), Positives = 32/50 (64%)
Frame = +3 / +2
Query: 9 GKLASHLEAKIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATV 158
G L +EAK++D T + L P Q GE+ +RGP +M+GY + EAT T+
Sbjct: 23 GTLVVGVEAKVIDVETQKHLPPSQLGEICIRGPHIMQGYFNNVEATEFTI 172
Score = 36.8 bits (74), Expect(3) = 8e-057
Identities = 14/19 (73%), Positives = 17/19 (89%)
Frame = +3 / +3
Query: 471 FVSAIPKSPAGKILRRELV 527
FV ++PKS AGKILRREL+
Sbjct: 483 FVESVPKSAAGKILRRELI 539
>gb|CK124865.1|CK124865 BES1824110k15 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010K1510 5-PRIME, mRNA sequence
Length = 630
Score = 215 bits (463), Expect = 3e-055
Identities = 87/168 (51%), Positives = 121/168 (72%)
Frame = +3 / +1
Query: 3 SVGKLASHLEAKIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATVDSEGWLKT 182
S G + + E K+VDP TG L GE+ +RGP +MKGY+ D ATAAT+D EGWL T
Sbjct: 73 SCGTVVRNAELKVVDPDTGLSLARNLPGEICIRGPQIMKGYLDDPVATAATIDVEGWLHT 252
Query: 183 GDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQL 362
GD+ Y ++D ++IVDR+KELIK+KG+QVPPAELE +L +HP I DAAV+P D+ AG++
Sbjct: 253 GDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLIAHPSIADAAVVPQKDDAAGEV 432
Query: 363 PMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSPAGK 506
P+AF+VR S++ E+ + +FV+KQV YK++ +V F AIPK G+
Sbjct: 433 PVAFVVRAADSDIAEEAIKEFVSKQVVFYKRLHKVYFTHAIPKFGVGE 576
>gb|BE454671.3|BE454671 HVSMEh0094P16f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0094P16f, mRNA sequence
Length = 685
Score = 212 bits (458), Expect = 2e-054
Identities = 85/168 (50%), Positives = 120/168 (71%)
Frame = +3 / +1
Query: 3 SVGKLASHLEAKIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATVDSEGWLKT 182
S G + + KIVDP TG LG Q GE+ +RG +MKGY+ D E+T T+D +GWL T
Sbjct: 178 SCGTVVRNAGLKIVDPDTGASLGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHT 357
Query: 183 GDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQL 362
GD+ ++D ++IVDRLKE+IKYKG+QV PAELE +L +HP+I DAAV+ D+ AG++
Sbjct: 358 GDIGIVDDDDEIFIVDRLKEIIKYKGFQVAPAELEALLITHPEIKDAAVVSLKDDLAGEV 537
Query: 363 PMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSPAGK 506
P+AF++R GS +TE + FVAK+V YK++ +V F +IPK+P+GK
Sbjct: 538 PIAFVMRIEGSEITEDDIKKFVAKEVVFYKRIHKVFFTDSIPKNPSGK 681
>gb|CA007491.1|CA007491 HU08C03r HU Hordeum vulgare subsp. vulgare cDNA clone HU08C03
5-PRIME, mRNA sequence
Length = 581
Score = 202 bits (435), Expect = 2e-051
Identities = 78/124 (62%), Positives = 97/124 (78%)
Frame = +3 / +1
Query: 3 SVGKLASHLEAKIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATVDSEGWLKT 182
+ G L+ + EAKIV P TGE L + GELW+RGP VMKGY + EAT +TV +GWLKT
Sbjct: 208 TAGLLSPNTEAKIVPPETGEALPVNRTGELWIRGPYVMKGYFKNTEATQSTVTPDGWLKT 387
Query: 183 GDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQL 362
GDLCY +EDG L++VDRLKELIKYKGYQ+PPAELE +L +HP++ D AVIP+PD + GQ
Sbjct: 388 GDLCYIDEDGYLFVVDRLKELIKYKGYQLPPAELEALLLTHPEVSDVAVIPFPDREVGQF 567
Query: 363 PMAF 374
PMA+
Sbjct: 568 PMAY 579
>gb|BQ763608.1|BQ763608 EBro02_SQ008_M23_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_M23 5', mRNA sequence
Length = 590
Score = 200 bits (431), Expect = 8e-051
Identities = 81/163 (49%), Positives = 114/163 (69%)
Frame = +3 / +3
Query: 3 SVGKLASHLEAKIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATVDSEGWLKT 182
S G + + KIVDP TG LG Q GE+ +RG +MKGY+ D E+T T+D +GWL T
Sbjct: 102 SCGTVVRNAGLKIVDPDTGASLGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHT 281
Query: 183 GDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQL 362
GD+ ++D ++IVDRLKE+IKYKG+QV PAELE +L +HP+I DAAV+ D+ AG++
Sbjct: 282 GDIGIVDDDDEIFIVDRLKEIIKYKGFQVAPAELEALLITHPEIKDAAVVSLKDDLAGEV 461
Query: 363 PMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPK 491
P+AF++R GS +TE + FVAK+ YK++ +V F +IPK
Sbjct: 462 PIAFVMRIEGSEITEDDIKKFVAKEGVFYKRIHKVFFTDSIPK 590
>gb|AV918175.1|AV918175 AV918175 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20b24 3', mRNA sequence
Length = 664
Score = 146 bits (314), Expect(3) = 3e-050
Identities = 57/97 (58%), Positives = 78/97 (80%)
Frame = +3 / -3
Query: 237 KELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQV 416
K + KG+Q+ PAELE +L SHP+I+DA VIP+PD++AG++P+A++VR P S+LTE V
Sbjct: 518 KS*LSTKGFQIAPAELEGLLLSHPEILDAVVIPFPDDEAGEVPIAYVVRSPASSLTEVDV 339
Query: 417 MDFVAKQVAPYKKVRRVAFVSAIPKSPAGKILRRELV 527
F+AKQV YK++RRV FV ++PKS AGKILRREL+
Sbjct: 338 QKFIAKQVTYYKRLRRVTFVESVPKSAAGKILRRELI 228
Score = 61.6 bits (128), Expect(3) = 3e-050
Identities = 23/33 (69%), Positives = 28/33 (84%)
Frame = +3 / -2
Query: 165 EGWLKTGDLCYFNEDGLLYIVDRLKELIKYKGY 263
+GWL TGDL YF +G L++VDRLKELIKYKG+
Sbjct: 591 QGWLHTGDLGYFE*EGQLFVVDRLKELIKYKGF 493
Score = 29.0 bits (57), Expect(3) = 3e-050
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +3 / -1
Query: 96 VRGPVVMKGYVGDDEATAATVDS 164
+RGP +M+GY + EAT T+ +
Sbjct: 658 IRGPHIMQGYFNNVEATEFTIQA 590
>gb|BE213865.1|BE213865 HV_CEb0001H21f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0001H21f, mRNA sequence
Length = 922
Score = 198 bits (426), Expect = 4e-050
Identities = 78/153 (50%), Positives = 114/153 (74%)
Frame = +3 / +1
Query: 66 LGPGQRGELWVRGPVVMKGYVGDDEATAATVDSEGWLKTGDLCYFNEDGLLYIVDRLKEL 245
LG Q GE VRG +MKGY+ D E+T T+D +GWL TGD+ ++D ++IVDRLKE+
Sbjct: 1 LGRNQPGERGVRGEHIMKGYLNDPESTKNTIDKDGWLHTGDIGIVDDDDEIFIVDRLKEI 180
Query: 246 IKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDF 425
+KYKG+QV PAELE +L +HP+I DAAV+ D+ AG++P+AF++R GS +TE + F
Sbjct: 181 MKYKGFQVAPAELEALLITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDIKKF 360
Query: 426 VAKQVAPYKKVRRVAFVSAIPKSPAGKILRREL 524
VAK+V YK++ +V F ++PK+P+G+ILR++L
Sbjct: 361 VAKEVGFYKRIHKVFFTDSMPKNPSGEILRKDL 459
>gb|BF623201.2|BF623201 HVSMEa0015K22f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0015K22f, mRNA sequence
Length = 817
Score = 179 bits (385), Expect(2) = 7e-046
Identities = 74/139 (53%), Positives = 98/139 (70%)
Frame = +3 / +3
Query: 36 KIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATVDSEGWLKTGDLCYFNEDGL 215
KIVDP TG L GE+ +RG +MKGY+ D EAT T D +GWL TGD+ Y ++D
Sbjct: 342 KIVDPDTGASLARNLPGEICIRGKQIMKGYLNDPEATKNTTDKDGWLHTGDIGYVDDDDE 521
Query: 216 LYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGS 395
++IVDRLKE+IKYKG+QVPPAELE +L +H +I DAAV+ DE G++P+AFIVR GS
Sbjct: 522 IFIVDRLKEIIKYKGFQVPPAELEPLLITHAEIKDAAVVSMQDELTGEVPVAFIVRIEGS 701
Query: 396 NLTEQQVMDFVAKQVAPYK 452
++E ++ F AK+V K
Sbjct: 702 EISESEIKQFGAKEVVSTK 758
Score = 24.9 bits (48), Expect(2) = 7e-046
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +3 / +3
Query: 462 RVAFVSAIPKSPAGK 506
+V F ++PKSPAG+
Sbjct: 765 QVFFADSVPKSPAGR 809
>gb|BQ766207.1|BQ766207 EBro08_SQ005_H07_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ005_H07 5', mRNA sequence
Length = 552
Score = 174 bits (375), Expect = 4e-043
Identities = 70/138 (50%), Positives = 98/138 (71%)
Frame = +3 / +3
Query: 3 SVGKLASHLEAKIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATVDSEGWLKT 182
S G + + KIVDP TG LG Q GE+ +RG +MKGY+ D E+T T+D +GWL T
Sbjct: 132 SCGTVVRNAGLKIVDPDTGASLGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHT 311
Query: 183 GDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQL 362
GD+ ++D ++IVDRLKE+IKYKG+QV PAELE +L +HP+I DAAV+ D+ AG++
Sbjct: 312 GDIGIVDDDDEIFIVDRLKEIIKYKGFQVAPAELEALLITHPEIKDAAVVSLKDDLAGEV 491
Query: 363 PMAFIVRKPGSNLTEQQV 416
P+AF++R GS +TE +
Sbjct: 492 PIAFVMRIEGSEITEDDI 545
>gb|AL510457.1|AL510457 AL510457 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04P21u 3',
mRNA sequence
Length = 508
Score = 173 bits (372), Expect = 1e-042
Identities = 69/125 (55%), Positives = 93/125 (74%)
Frame = +3 / +1
Query: 3 SVGKLASHLEAKIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATVDSEGWLKT 182
S G + + E K+VDP TG L GE+ +RGP +MKGY+ D ATAAT+D EGWL T
Sbjct: 124 SCGTVVRNAELKVVDPDTGLSLARNLPGEICIRGPQIMKGYLDDPVATAATIDVEGWLHT 303
Query: 183 GDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQL 362
GD+ Y ++D ++IVDR+KELIK+KG+QVPPAELE +L +HP I DAAV+P D+ AG++
Sbjct: 304 GDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLIAHPSIADAAVVPQKDDAAGEV 483
Query: 363 PMAFI 377
P+AF+
Sbjct: 484 PVAFV 498
>gb|CB882386.1|CB882386 HL01L15w HL Hordeum vulgare subsp. vulgare cDNA clone HL01L15
3-PRIME, mRNA sequence
Length = 490
Score = 171 bits (368), Expect = 4e-042
Identities = 67/120 (55%), Positives = 94/120 (78%)
Frame = +3 / +1
Query: 165 EGWLKTGDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPD 344
+GWL TGD+ Y ++D ++IVDRLKE+IKYKG+QVPPAELE +L +H +I DAAV+ D
Sbjct: 7 DGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHAEIKDAAVVSMQD 186
Query: 345 EDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSPAGKILRREL 524
E G++P+AFIVR GS ++E ++ FVAK+V YK++ +V F ++PKSPAGKILR++L
Sbjct: 187 ELTGEVPVAFIVRIEGSEISESEIKQFVAKEVVFYKRIHKVFFADSVPKSPAGKILRKDL 366
>gb|AL507037.1|AL507037 AL507037 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04P21T 5',
mRNA sequence
Length = 700
Score = 145 bits (311), Expect(2) = 1e-038
Identities = 59/103 (57%), Positives = 81/103 (78%)
Frame = +3 / -2
Query: 216 LYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGS 395
+ IVDR+KELIK+KG+QVPPAELE L +HP I DAAV+P D+ AG++P+AF+VR S
Sbjct: 624 VXIVDRVKELIKFKGFQVPPAELEAXLIAHPSIADAAVVPQKDDAAGEVPVAFVVRAADS 445
Query: 396 NLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSPAGKILRREL 524
++ E+ + +FV+KQV YK++ +V F AIPKS +GKILR+EL
Sbjct: 444 DIAEEAIKEFVSKQVVFYKRLHKVYFTHAIPKSASGKILRKEL 316
Score = 34.5 bits (69), Expect(2) = 1e-038
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +3 / -3
Query: 156 VDSEGWLKTGDLCYFNEDG 212
+D EGWL TGD+ Y ++DG
Sbjct: 686 IDVEGWLHTGDIGYVDDDG 630
>gb|BI956935.1|BI956935 HVSMEn0006D04f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0006D04f, mRNA sequence
Length = 654
Score = 147 bits (316), Expect = 6e-035
Identities = 59/125 (47%), Positives = 86/125 (68%)
Frame = +3 / +1
Query: 3 SVGKLASHLEAKIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATVDSEGWLKT 182
S G + + KIVDP TG LG Q GE+ +RG +MKGY+ D ++T T+D +GWL T
Sbjct: 271 SCGTVVRNAGLKIVDPDTGASLGRNQPGEICIRGEQIMKGYLNDPKSTKNTIDKDGWLHT 450
Query: 183 GDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQL 362
GD+ ++D ++IVDRLKE+IKYKG+QV PA+LE +L +HP+I D V+ D+ A ++
Sbjct: 451 GDIGIVDDDDEIFIVDRLKEIIKYKGFQVAPAKLEALLITHPEIQDXGVVFLKDDLACEV 630
Query: 363 PMAFI 377
P+ F+
Sbjct: 631 PIPFV 645
>gb|AL502418.1|AL502418 AL502418 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07I12u 3', mRNA sequence
Length = 592
Score = 119 bits (254), Expect(2) = 1e-032
Identities = 45/74 (60%), Positives = 62/74 (83%)
Frame = +3 / -1
Query: 306 PDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAI 485
P+I+DA VIP+PD++AG++P+A++VR P S+LTE V F+AKQV YK++RRV FV ++
Sbjct: 520 PEILDAVVIPFPDDEAGEVPIAYVVRSPASSLTEVDVQKFIAKQVTYYKRLRRVTFVESV 341
Query: 486 PKSPAGKILRRELV 527
PKS AGKILRREL+
Sbjct: 340 PKSAAGKILRRELI 299
Score = 40.5 bits (82), Expect(2) = 1e-032
Identities = 16/24 (66%), Positives = 19/24 (79%)
Frame = +3 / -3
Query: 237 KELIKYKGYQVPPAELEHILNSHP 308
KELIK KG+Q+ PAE E +L SHP
Sbjct: 590 KELIKSKGFQIAPAEXEGLLLSHP 519
>gb|CV059902.1|CV059902 BNEL52b8 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL52b8 5' similar to Putative
AMP-binding protein, mRNA sequence
Length = 548
Score = 137 bits (294), Expect = 6e-032
Identities = 53/84 (63%), Positives = 72/84 (85%)
Frame = +3 / +1
Query: 291 ILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVA 470
+L +HP++ D AVIP+PD + GQ PMA++VRK GSNL+ Q+VM+FVAKQVAPYKKVR+VA
Sbjct: 1 LLLTHPEVSDVAVIPFPDREVGQFPMAYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVA 180
Query: 471 FVSAIPKSPAGKILRRELVEXAVS 542
FV+ IPK+ +GKILR++L++ A S
Sbjct: 181 FVTDIPKNASGKILRKDLIKLATS 252
>gb|CV061694.1|CV061694 BNEL70h10 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL70h10 5' similar to Putative
AMP-binding protein, mRNA sequence
Length = 568
Score = 137 bits (294), Expect = 6e-032
Identities = 53/84 (63%), Positives = 72/84 (85%)
Frame = +3 / +1
Query: 291 ILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVA 470
+L +HP++ D AVIP+PD + GQ PMA++VRK GSNL+ Q+VM+FVAKQVAPYKKVR+VA
Sbjct: 1 LLLTHPEVSDVAVIPFPDREVGQFPMAYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVA 180
Query: 471 FVSAIPKSPAGKILRRELVEXAVS 542
FV+ IPK+ +GKILR++L++ A S
Sbjct: 181 FVTDIPKNASGKILRKDLIKLATS 252
>gb|BI959839.1|BI959839 HVSMEn0021P10f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0021P10f, mRNA sequence
Length = 657
Score = 98.2 bits (208), Expect(2) = 7e-030
Identities = 37/75 (49%), Positives = 57/75 (76%)
Frame = +3 / -1
Query: 300 SHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVS 479
+HP+I DAAV+ D+ AG++P+AF++R GS +TE + FVAK+V YK++ +V F
Sbjct: 549 THPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDIKKFVAKEVVFYKRIHKVFFTD 370
Query: 480 AIPKSPAGKILRREL 524
+IPK+P+GKILR++L
Sbjct: 369 SIPKNPSGKILRKDL 325
Score = 52.4 bits (108), Expect(2) = 7e-030
Identities = 20/29 (68%), Positives = 26/29 (89%)
Frame = +3 / -3
Query: 201 NEDGLLYIVDRLKELIKYKGYQVPPAELE 287
++D ++IVDRLKE+IKYKG+QV PAELE
Sbjct: 649 DDDDEIFIVDRLKEIIKYKGFQVAPAELE 563
>gb|CA005904.1|CA005904 HU08C03u HU Hordeum vulgare subsp. vulgare cDNA clone HU08C03
3-PRIME, mRNA sequence
Length = 484
Score = 127 bits (273), Expect = 5e-029
Identities = 50/77 (64%), Positives = 66/77 (85%)
Frame = +3 / -1
Query: 312 IMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPK 491
+ D AVIP+PD + GQ PMA++VRK GSNL+ Q+VM+FVAKQVAPYKKVR+VAFV+ IPK
Sbjct: 484 VSDVAVIPFPDREVGQFPMAYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVAFVTDIPK 305
Query: 492 SPAGKILRRELVEXAVS 542
+ +GKILR++L++ A S
Sbjct: 304 NASGKILRKDLIKLATS 254
>gb|CA016130.1|CA016130 HV10D20u HV Hordeum vulgare subsp. vulgare cDNA clone HV10D20
3-PRIME, mRNA sequence
Length = 491
Score = 127 bits (271), Expect = 1e-028
Identities = 50/75 (66%), Positives = 65/75 (86%)
Frame = +3 / -3
Query: 318 DAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSP 497
D AVIP+PD + GQ PMA++VRK GSNL+ Q+VM+FVAKQVAPYKKVR+VAFV+ IPK+
Sbjct: 489 DVAVIPFPDREVGQFPMAYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVAFVTDIPKNA 310
Query: 498 AGKILRRELVEXAVS 542
+GKILR++L++ A S
Sbjct: 309 SGKILRKDLIKLATS 265
>gb|AL510866.1|AL510866 AL510866 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY06D22u 3',
mRNA sequence
Length = 513
Score = 121 bits (258), Expect = 6e-027
Identities = 47/73 (64%), Positives = 63/73 (86%)
Frame = +3 / -1
Query: 324 AVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSPAG 503
A+IP+PD + GQ PM ++VRK GSNL+ Q+VM+FVAKQVAPYKKVR+VAFV+ IPK+ +G
Sbjct: 492 ALIPFPDREVGQFPMXYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVAFVTDIPKNASG 313
Query: 504 KILRRELVEXAVS 542
KILR++L++ A S
Sbjct: 312 KILRKDLIKLATS 274
>gb|BQ465081.1|BQ465081 HU02J12r HU Hordeum vulgare subsp. vulgare cDNA clone HU02J12
5-PRIME, mRNA sequence
Length = 633
Score = 117 bits (251), Expect = 6e-026
Identities = 47/89 (52%), Positives = 62/89 (69%)
Frame = +3 / +2
Query: 3 SVGKLASHLEAKIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATVDSEGWLKT 182
S G + + E KIVDP TG L GE+ +RG +MKGY+ D EAT T+D +GWL T
Sbjct: 365 SCGTVVRNAELKIVDPDTGASLARNLPGEICIRGKQIMKGYLNDPEATKNTIDKDGWLHT 544
Query: 183 GDLCYFNEDGLLYIVDRLKELIKYKGYQV 269
GD+ Y ++D ++IVDRLKE+IKYKG+QV
Sbjct: 545 GDIGYVDDDDEIFIVDRLKEIIKYKGFQV 631
>gb|AV833853.1|AV833853 AV833853 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags8m01, mRNA sequence
Length = 599
Score = 90.0 bits (190), Expect(2) = 7e-025
Identities = 35/71 (49%), Positives = 53/71 (74%)
Frame = +3 / +3
Query: 312 IMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPK 491
I DAAV+ D+ AG++P+AF++R GS +TE + FVAK+V YK++ +V F +IPK
Sbjct: 168 IKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDIKKFVAKEVVFYKRIHKVFFTDSIPK 347
Query: 492 SPAGKILRREL 524
+P+GKILR++L
Sbjct: 348 NPSGKILRKDL 380
Score = 43.7 bits (89), Expect(2) = 7e-025
Identities = 16/21 (76%), Positives = 20/21 (95%)
Frame = +3 / +1
Query: 216 LYIVDRLKELIKYKGYQVPPA 278
++IVDRLKE+IKYKG+QV PA
Sbjct: 70 IFIVDRLKEIIKYKGFQVAPA 132
>gb|BG344702.1|BG344702 HVSMEg0015E07f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0015E07f, mRNA sequence
Length = 875
Score = 112 bits (239), Expect = 2e-024
Identities = 45/89 (50%), Positives = 61/89 (68%)
Frame = +3 / +1
Query: 3 SVGKLASHLEAKIVDPSTGEVLGPGQRGELWVRGPVVMKGYVGDDEATAATVDSEGWLKT 182
S G + + KIVDP TG +LG Q GE+ +RG +MKGY+ D E+T T+D +GWL T
Sbjct: 427 SCGTVVRNAGLKIVDPDTGXLLGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHT 606
Query: 183 GDLCYFNEDGLLYIVDRLKELIKYKGYQV 269
GD+ ++D ++IVDRLKE+IKYKG V
Sbjct: 607 GDIGIVDDDDEIFIVDRLKEIIKYKGLXV 693
>gb|BQ757736.1|BQ757736 EBem10_SQ005_B05_R embryo, 2 Day germination, no treatment, cv
Optic, EBem10 Hordeum vulgare subsp. vulgare cDNA clone
EBem10_SQ005_B05 5', mRNA sequence
Length = 338
Score = 100 bits (214), Expect = 7e-021
Identities = 42/82 (51%), Positives = 60/82 (73%)
Frame = +3 / +2
Query: 318 DAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSP 497
+AAV+ Y D+ AG+LP+AF+V++ GS + E Q+ DFVAKQV YK++ RV V +IPK+
Sbjct: 2 EAAVVGYADDQAGELPVAFVVQRSGSKVHEAQIKDFVAKQVVHYKRIHRVFLVDSIPKNA 181
Query: 498 AGKILRRELVEXAVSMGTXKL* 563
AGKILR++L + A+ KL*
Sbjct: 182 AGKILRKDLAKLALHRINAKL* 247
>gb|BQ766052.1|BQ766052 EBro03_SQ008_L23_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ008_L23 5', mRNA sequence
Length = 656
Score = 91.3 bits (193), Expect(2) = 9e-021
Identities = 40/113 (35%), Positives = 62/113 (54%)
Frame = +3 / +1
Query: 135 DEATAATVDSEGWLKTGDLCYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDI 314
D A A GWL++GDL + DG + + DR K++I G + E+E L HP +
Sbjct: 175 DAAATAEAMKGGWLRSGDLGVRHPDGYVQLKDRSKDIIISGGENISSIEVESALFGHPAV 354
Query: 315 MDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAF 473
+DAAV+ PD+ G+ P AF+ K G++ TE +++F ++ Y R V F
Sbjct: 355 LDAAVVARPDDHWGETPCAFVTLKDGASATEADMIEFCRARLPRYMAPRTVLF 513
Score = 28.6 bits (56), Expect(2) = 9e-021
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +3 / +1
Query: 84 GELWVRGPVVMKGYVGDDEATA 149
GE+ +RG VM GY D ATA
Sbjct: 127 GEVMLRGNTVMSGYYKDAAATA 192
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 320,156,724
Number of Sequences: 312970
Number of extensions: 4349972
Number of successful extensions: 44770
Number of sequences better than 1.0e-020: 38
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8610591927
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)