TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1716323.2.1
(724 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU986484.1|BU986484 HF12A02r HF Hordeum vulgare subsp. v... 303 5e-082
gb|BF064865.2|BF064865 HV_CEb0021D12f Hordeum vulgare seedl... 232 1e-060
gb|BG414540.1|BG414540 HVSMEk0002N03f Hordeum vulgare testa... 200 6e-051
gb|BG418054.1|BG418054 HVSMEk0021B11f Hordeum vulgare testa... 200 8e-051
gb|AV918175.1|AV918175 AV918175 K. Sato unpublished cDNA li... 149 2e-046
gb|AV916926.1|AV916926 AV916926 K. Sato unpublished cDNA li... 164 1e-045
gb|BE213865.1|BE213865 HV_CEb0001H21f Hordeum vulgare seedl... 177 8e-044
gb|CK124865.1|CK124865 BES1824110k15 BES1824 Hordeum vulgar... 174 4e-043
gb|BE454671.3|BE454671 HVSMEh0094P16f Hordeum vulgare 5-45 ... 171 3e-042
gb|CB882386.1|CB882386 HL01L15w HL Hordeum vulgare subsp. v... 167 5e-041
gb|BQ763608.1|BQ763608 EBro02_SQ008_M23_R root, 3 week, hyd... 159 1e-038
gb|AL507037.1|AL507037 AL507037 Hordeum vulgare Barke devel... 145 1e-037
gb|BF623201.2|BF623201 HVSMEa0015K22f Hordeum vulgare seedl... 146 3e-036
gb|AL502418.1|AL502418 AL502418 Hordeum vulgare Barke roots... 121 2e-033
gb|CA007491.1|CA007491 HU08C03r HU Hordeum vulgare subsp. v... 141 5e-033
gb|CV059902.1|CV059902 BNEL52b8 Barley EST endosperm librar... 137 8e-032
gb|CV061694.1|CV061694 BNEL70h10 Barley EST endosperm libra... 137 8e-032
gb|BQ766207.1|BQ766207 EBro08_SQ005_H07_R root, 3 week, dro... 133 8e-031
gb|AL510457.1|AL510457 AL510457 Hordeum vulgare Barke devel... 133 1e-030
gb|BI959839.1|BI959839 HVSMEn0021P10f Hordeum vulgare rachi... 98 6e-030
gb|CA005904.1|CA005904 HU08C03u HU Hordeum vulgare subsp. v... 127 7e-029
gb|CA016130.1|CA016130 HV10D20u HV Hordeum vulgare subsp. v... 126 1e-028
gb|AL510866.1|AL510866 AL510866 Hordeum vulgare Barke devel... 120 8e-027
gb|AV833853.1|AV833853 AV833853 K. Sato unpublished cDNA li... 90 7e-025
gb|BI956935.1|BI956935 HVSMEn0006D04f Hordeum vulgare rachi... 106 1e-022
gb|BQ757736.1|BQ757736 EBem10_SQ005_B05_R embryo, 2 Day ger... 100 7e-021
>gb|BU986484.1|BU986484 HF12A02r HF Hordeum vulgare subsp. vulgare cDNA clone HF12A02
5-PRIME, mRNA sequence
Length = 577
Score = 303 bits (657), Expect = 5e-082
Identities = 127/154 (82%), Positives = 139/154 (90%)
Frame = +3 / +1
Query: 78 MIFSGYVGDDEATAATVDSEGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELE 257
++ GYVGDD+ATA TVDSEGWLKTG YFNEDG LYIVDRLKELIKYKGYQVPPAELE
Sbjct: 16 LVMKGYVGDDKATAETVDSEGWLKTGDLCYFNEDGFLYIVDRLKELIKYKGYQVPPAELE 195
Query: 258 HILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRV 437
HIL SHP+I DAAVI YPDED GQLPMAFIVR+PGS+LT +QVMD+VAK VAPYKKVRRV
Sbjct: 196 HILQSHPEIADAAVIGYPDEDVGQLPMAFIVRQPGSSLTGKQVMDYVAKHVAPYKKVRRV 375
Query: 438 AFVSAIPKSPAGKILRRELVEQAVSMGTSKL*SL 539
AFV+AIPKSPAGKILRRELV+QA+SMG SKL* +
Sbjct: 376 AFVAAIPKSPAGKILRRELVQQAMSMGASKL*DV 477
Score = 240 bits (518), Expect = 8e-063
Identities = 95/148 (64%), Positives = 113/148 (76%)
Frame = -3 / -2
Query: 533 SKLGGTHGHGLLYKLPPQDLPSRRFGDCRNKGHSTNFLVGCDLFSHEIHDLLLGKV*PWF 354
SKLGGTHGHGLL+KLP QDLP RRFG CR++GHS +FLVG ++FSH IHDL K * W
Sbjct: 471 SKLGGTHGHGLLHKLPSQDLPRRRFGYCRDEGHSADFLVGRNMFSHVIHDLFPSKA*TWL 292
Query: 353 PHYKSHWELPGVFVWIRNNCCVHDIRMRIQDMLQFGRRYLISLVLYQLFQPINNVE*PIL 174
PHYK HW+LP +F+WI +NC + + RM + +MLQF R+L+S VLYQL Q INNVE I
Sbjct: 291 PHYKGHWQLPNIFIWISDNCSISNFRM*L*NMLQFSWRHLVSFVLYQLLQTINNVEKSIF 112
Query: 173 VEVAXXTGFQPAF*IHCCCSSLVVTDIT 90
+EVA T FQPAF*+HC S VVTDIT
Sbjct: 111 IEVAKITSFQPAF*VHCFRSCFVVTDIT 28
Score = 211 bits (455), Expect = 4e-054
Identities = 96/131 (73%), Positives = 99/131 (75%)
Frame = -1 / -3
Query: 532 QSLEVPMDTACSTSSRRKIFPAGDLGIAETKATRRTFL*GATCLATKSMTCCSVRFDPGF 353
QSLE PMD AC TSSRRKIFPAGDLGIA TKATRRTFL*GATCLAT SMTC VR +PG
Sbjct: 470 QSLEAPMDMACCTSSRRKIFPAGDLGIAATKATRRTFL*GATCLAT*SMTCFPVRLEPGC 291
Query: 352 LTIKAIGSCPXXXXXXXXXXXXXXXG*EFRICSSSAGGT*YPLYFINSFNRSTM*SNPSS 173
LTIKAIGSCP G + RICSSSAGGT YPLYFINSF RSTM* NPSS
Sbjct: 290 LTIKAIGSCPTSSSGYPITAASAISGCDCRICSSSAGGTWYPLYFINSFKRSTM*RNPSS 111
Query: 172 LK*XXXPVFNQ 140
LK* PVF+Q
Sbjct: 110 LK*QRSPVFSQ 78
Score = 143 bits (307), Expect(2) = 6e-035
Identities = 71/125 (56%), Positives = 80/125 (64%)
Frame = +1 / +2
Query: 88 QVMSVTTRLLQQQWIQKAG*KPVXXATSTRMGYSTLLIG*KS**STRDIRYLLPNWXXXX 267
+VMSVTT+ L++QW QKAG*K V ATS +M +STLLI *+S**ST+D R L NW
Sbjct: 26 KVMSVTTKQLRKQWTQKAG*KLVIFATSMKMDFSTLLIV*RS**STKDTRCLQLNWSIFY 205
Query: 268 XXXXXXWTQQLFLIQTKTPGNSQWLL**GNQGQTLPSNKSWISWLNRSHPTRKFVEWPLF 447
QL IQ K GN QW L**GNQ Q L NKSWI+WLN PTRK EWP
Sbjct: 206 NHILKLLMLQLSDIQMKMLGNCQWPL**GNQVQALLGNKSWITWLNMLRPTRKSAEWPSS 385
Query: 448 LQSPN 462
Q PN
Sbjct: 386 RQYPN 400
Score = 100 bits (212), Expect(2) = 3e-039
Identities = 46/70 (65%), Positives = 48/70 (68%)
Frame = +2 / +3
Query: 326 ATPNGFYSEETRVKPYRATSHGFRG*TGRTLQESSSSGLCFCNPQIACWEDLAAGACRAG 505
AT NG YSE TR KPY TSHG RG*T LQES SGL N QI+C EDLA GAC A
Sbjct: 264 ATANGLYSEATRFKPYWETSHGLRG*TCCALQESPPSGLRRGNTQISCGEDLATGACAAS 443
Query: 506 RVHGYLQALK 535
VHG LQAL+
Sbjct: 444 HVHGCLQALR 473
Score = 81.7 bits (172), Expect(2) = 3e-039
Identities = 37/58 (63%), Positives = 40/58 (68%)
Frame = +2 / +3
Query: 89 RLCR*RRGYCSNSGFRRLVENRXXXLLQRGWVTLHC*SVERVDKVQGISGTSCRTGAY 262
RLCR*R+ C NSG RRL EN LLQ W++LHC*S E VDKVQ I G S TGAY
Sbjct: 27 RLCR*RQSNCGNSGLRRLAENW*SLLLQ*RWISLHC*SFEGVDKVQRIPGASS*TGAY 200
Score = 63.4 bits (132), Expect(3) = 9e-024
Identities = 25/40 (62%), Positives = 29/40 (72%)
Frame = -2 / -1
Query: 537 NFKAWRYPWTRPALQAPAARSSQQAIWGLQKQRPLDELSC 418
+ KAWR+PWT A QAP ARSS Q IW L ++RPL LSC
Sbjct: 475 HLKAWRHPWTWLAAQAPVARSSPQEIWVLPRRRPLGGLSC 356
Score = 57.4 bits (119), Expect(3) = 9e-024
Identities = 27/48 (56%), Positives = 30/48 (62%)
Frame = -2 / -1
Query: 282 YQDENSGYAPVRQEVPDIPCTLSTLSTDQQCRVTHPR*SSXXXRFSTS 139
+QD YAPV+ E P I CTLST S DQQCR H *SS +FS S
Sbjct: 220 FQDVIVEYAPVQLEAPGILCTLSTPSNDQQCREIHLH*SSKDHQFSAS 77
Score = 26.7 bits (52), Expect(3) = 9e-024
Identities = 12/18 (66%), Positives = 16/18 (88%)
Frame = -2 / -1
Query: 378 AR*GLTLVSSL*KPLGVA 325
++*GL LV+SL*+PL VA
Sbjct: 316 SQ*GLNLVASL*RPLAVA 263
Score = 24.0 bits (46), Expect(2) = 6e-035
Identities = 8/9 (88%), Positives = 9/9 (100%)
Frame = +1 / +2
Query: 514 WVPPSFEV* 540
WVPPSFE+*
Sbjct: 452 WVPPSFEM* 478
>gb|BF064865.2|BF064865 HV_CEb0021D12f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0021D12f, mRNA sequence
Length = 806
Score = 232 bits (502), Expect = 1e-060
Identities = 92/144 (63%), Positives = 118/144 (81%)
Frame = +3 / +3
Query: 81 IFSGYVGDDEATAATVDSEGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELEH 260
+ GY + EAT +TV +GWLKTG Y +EDG L++VDRLKELIKYKGYQVPPAELE
Sbjct: 186 VMKGYFKNTEATQSTVTPDGWLKTGDLCYIDEDGYLFVVDRLKELIKYKGYQVPPAELEA 365
Query: 261 ILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVA 440
+L +HP++ D AVIP+PD + GQ PMA++VRK GSNL+ Q+VM+FVAKQVAPYKKVR+VA
Sbjct: 366 LLLTHPEVSDVAVIPFPDREVGQFPMAYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVA 545
Query: 441 FVSAIPKSPAGKILRRELVEQAVS 512
FV+ IPK+ +GKILR++L++ A S
Sbjct: 546 FVTDIPKNASGKILRKDLIKLATS 617
>gb|BG414540.1|BG414540 HVSMEk0002N03f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0002N03f, mRNA sequence
Length = 876
Score = 200 bits (432), Expect = 6e-051
Identities = 78/126 (61%), Positives = 103/126 (81%)
Frame = +3 / +3
Query: 135 EGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPD 314
+GWL TG YF+E+G L++VDRLKELIKYKG+Q+ PAELE +L SHP+I+DA VIP+PD
Sbjct: 294 QGWLHTGDLGYFDEEGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPD 473
Query: 315 EDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSPAGKILRREL 494
++AG++P+A++VR P S+LTE V F+AKQV YK++RRV FV ++PKS AGKILRREL
Sbjct: 474 DEAGEVPIAYVVRSPASSLTEVDVQKFIAKQVTYYKRLRRVTFVESVPKSAAGKILRREL 653
Query: 495 VEQAVS 512
+ Q S
Sbjct: 654 IAQVRS 671
>gb|BG418054.1|BG418054 HVSMEk0021B11f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0021B11f, mRNA sequence
Length = 491
Score = 200 bits (431), Expect = 8e-051
Identities = 81/99 (81%), Positives = 87/99 (87%)
Frame = +3 / +1
Query: 78 MIFSGYVGDDEATAATVDSEGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELE 257
++ GYVGDD+ATA TVDSEGWLKTG YFNEDG LYIVDRLKELIKYKGYQ PPAELE
Sbjct: 187 LVMKGYVGDDKATAETVDSEGWLKTGDLCYFNEDGFLYIVDRLKELIKYKGYQGPPAELE 366
Query: 258 HILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLT 374
HIL SHP+I DAAVI YPDED GQLPMAFIVR+PGS+LT
Sbjct: 367 HILQSHPEIADAAVIGYPDEDVGQLPMAFIVRQPGSSLT 483
Score = 134 bits (288), Expect = 4e-031
Identities = 54/94 (57%), Positives = 66/94 (70%)
Frame = -3 / -3
Query: 371 KV*PWFPHYKSHWELPGVFVWIRNNCCVHDIRMRIQDMLQFGRRYLISLVLYQLFQPINN 192
K * W PHYK HW+LP +F+WI NC + + RM + +MLQF R L+S VLYQL Q INN
Sbjct: 480 KA*TWLPHYKGHWQLPNIFIWISYNCSISNFRM*L*NMLQFSWRPLVSFVLYQLLQTINN 301
Query: 191 VE*PILVEVAXXTGFQPAF*IHCCCSSLVVTDIT 90
VE I +EVA T F+PAF*+HC S VVT+IT
Sbjct: 300 VEKSIFIEVAKITSFKPAF*VHCFRSCFVVTNIT 199
Score = 106 bits (226), Expect = 1e-022
Identities = 49/81 (60%), Positives = 52/81 (64%)
Frame = -1 / -1
Query: 382 CCSVRFDPGFLTIKAIGSCPXXXXXXXXXXXXXXXG*EFRICSSSAGGT*YPLYFINSFN 203
C VR +PG LTIKAIGSCP G + RICSSSAGG YPLYFINSF
Sbjct: 491 CFPVRLEPGCLTIKAIGSCPTSSSGYPITAASAISGCDCRICSSSAGGPWYPLYFINSFK 312
Query: 202 RSTM*SNPSSLK*XXXPVFNQ 140
RSTM* NPSSLK* PV +Q
Sbjct: 311 RSTM*RNPSSLK*QRSPVLSQ 249
>gb|AV918175.1|AV918175 AV918175 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20b24 3', mRNA sequence
Length = 664
Score = 149 bits (319), Expect(2) = 2e-046
Identities = 59/102 (57%), Positives = 80/102 (78%)
Frame = +3 / -3
Query: 207 KELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQV 386
K + KG+Q+ PAELE +L SHP+I+DA VIP+PD++AG++P+A++VR P S+LTE V
Sbjct: 518 KS*LSTKGFQIAPAELEGLLLSHPEILDAVVIPFPDDEAGEVPIAYVVRSPASSLTEVDV 339
Query: 387 MDFVAKQVAPYKKVRRVAFVSAIPKSPAGKILRRELVEQAVS 512
F+AKQV YK++RRV FV ++PKS AGKILRREL+ Q S
Sbjct: 338 QKFIAKQVTYYKRLRRVTFVESVPKSAAGKILRRELIAQVRS 213
Score = 57.0 bits (118), Expect(2) = 2e-046
Identities = 21/33 (63%), Positives = 26/33 (78%)
Frame = +3 / -2
Query: 135 EGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGY 233
+GWL TG YF +G L++VDRLKELIKYKG+
Sbjct: 591 QGWLHTGDLGYFE*EGQLFVVDRLKELIKYKGF 493
>gb|AV916926.1|AV916926 AV916926 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20b24 5', mRNA sequence
Length = 547
Score = 164 bits (353), Expect(2) = 1e-045
Identities = 62/101 (61%), Positives = 84/101 (83%)
Frame = +3 / +2
Query: 135 EGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPD 314
+GWL TG YF+E+G L++VDRLKELIKYKG+Q+ PAELE +L SHP+I+DA VIP+PD
Sbjct: 176 QGWLHTGDLGYFDEEGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPD 355
Query: 315 EDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRV 437
++AG++P+A++VR P S+LTE V F+AKQV YK++RRV
Sbjct: 356 DEAGEVPIAYVVRSPASSLTEVDVQKFIAKQVTYYKRLRRV 478
Score = 38.6 bits (78), Expect(2) = 1e-045
Identities = 15/21 (71%), Positives = 18/21 (85%)
Frame = +3 / +3
Query: 441 FVSAIPKSPAGKILRRELVEQ 503
FV ++PKS AGKILRREL+ Q
Sbjct: 483 FVESVPKSAAGKILRRELIAQ 545
>gb|BE213865.1|BE213865 HV_CEb0001H21f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0001H21f, mRNA sequence
Length = 922
Score = 177 bits (380), Expect = 8e-044
Identities = 68/138 (49%), Positives = 102/138 (73%)
Frame = +3 / +1
Query: 81 IFSGYVGDDEATAATVDSEGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELEH 260
I GY+ D E+T T+D +GWL TG ++D ++IVDRLKE++KYKG+QV PAELE
Sbjct: 46 IMKGYLNDPESTKNTIDKDGWLHTGDIGIVDDDDEIFIVDRLKEIMKYKGFQVAPAELEA 225
Query: 261 ILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVA 440
+L +HP+I DAAV+ D+ AG++P+AF++R GS +TE + FVAK+V YK++ +V
Sbjct: 226 LLITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDIKKFVAKEVGFYKRIHKVF 405
Query: 441 FVSAIPKSPAGKILRREL 494
F ++PK+P+G+ILR++L
Sbjct: 406 FTDSMPKNPSGEILRKDL 459
>gb|CK124865.1|CK124865 BES1824110k15 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010K1510 5-PRIME, mRNA sequence
Length = 630
Score = 174 bits (375), Expect = 4e-043
Identities = 70/132 (53%), Positives = 97/132 (73%)
Frame = +3 / +1
Query: 81 IFSGYVGDDEATAATVDSEGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELEH 260
I GY+ D ATAAT+D EGWL TG Y ++D ++IVDR+KELIK+KG+QVPPAELE
Sbjct: 181 IMKGYLDDPVATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEA 360
Query: 261 ILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVA 440
+L +HP I DAAV+P D+ AG++P+AF+VR S++ E+ + +FV+KQV YK++ +V
Sbjct: 361 LLIAHPSIADAAVVPQKDDAAGEVPVAFVVRAADSDIAEEAIKEFVSKQVVFYKRLHKVY 540
Query: 441 FVSAIPKSPAGK 476
F AIPK G+
Sbjct: 541 FTHAIPKFGVGE 576
>gb|BE454671.3|BE454671 HVSMEh0094P16f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0094P16f, mRNA sequence
Length = 685
Score = 171 bits (369), Expect = 3e-042
Identities = 67/132 (50%), Positives = 96/132 (72%)
Frame = +3 / +1
Query: 81 IFSGYVGDDEATAATVDSEGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELEH 260
I GY+ D E+T T+D +GWL TG ++D ++IVDRLKE+IKYKG+QV PAELE
Sbjct: 286 IMKGYLNDPESTKNTIDKDGWLHTGDIGIVDDDDEIFIVDRLKEIIKYKGFQVAPAELEA 465
Query: 261 ILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVA 440
+L +HP+I DAAV+ D+ AG++P+AF++R GS +TE + FVAK+V YK++ +V
Sbjct: 466 LLITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDIKKFVAKEVVFYKRIHKVF 645
Query: 441 FVSAIPKSPAGK 476
F +IPK+P+GK
Sbjct: 646 FTDSIPKNPSGK 681
>gb|CB882386.1|CB882386 HL01L15w HL Hordeum vulgare subsp. vulgare cDNA clone HL01L15
3-PRIME, mRNA sequence
Length = 490
Score = 167 bits (360), Expect = 5e-041
Identities = 66/120 (55%), Positives = 92/120 (76%)
Frame = +3 / +1
Query: 135 EGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPD 314
+GWL TG Y ++D ++IVDRLKE+IKYKG+QVPPAELE +L +H +I DAAV+ D
Sbjct: 7 DGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHAEIKDAAVVSMQD 186
Query: 315 EDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSPAGKILRREL 494
E G++P+AFIVR GS ++E ++ FVAK+V YK++ +V F ++PKSPAGKILR++L
Sbjct: 187 ELTGEVPVAFIVRIEGSEISESEIKQFVAKEVVFYKRIHKVFFADSVPKSPAGKILRKDL 366
>gb|BQ763608.1|BQ763608 EBro02_SQ008_M23_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_M23 5', mRNA sequence
Length = 590
Score = 159 bits (342), Expect = 1e-038
Identities = 63/127 (49%), Positives = 90/127 (70%)
Frame = +3 / +3
Query: 81 IFSGYVGDDEATAATVDSEGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELEH 260
I GY+ D E+T T+D +GWL TG ++D ++IVDRLKE+IKYKG+QV PAELE
Sbjct: 210 IMKGYLNDPESTKNTIDKDGWLHTGDIGIVDDDDEIFIVDRLKEIIKYKGFQVAPAELEA 389
Query: 261 ILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVA 440
+L +HP+I DAAV+ D+ AG++P+AF++R GS +TE + FVAK+ YK++ +V
Sbjct: 390 LLITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDIKKFVAKEGVFYKRIHKVF 569
Query: 441 FVSAIPK 461
F +IPK
Sbjct: 570 FTDSIPK 590
>gb|AL507037.1|AL507037 AL507037 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04P21T 5',
mRNA sequence
Length = 700
Score = 145 bits (311), Expect(2) = 1e-037
Identities = 59/103 (57%), Positives = 81/103 (78%)
Frame = +3 / -2
Query: 186 LYIVDRLKELIKYKGYQVPPAELEHILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGS 365
+ IVDR+KELIK+KG+QVPPAELE L +HP I DAAV+P D+ AG++P+AF+VR S
Sbjct: 624 VXIVDRVKELIKFKGFQVPPAELEAXLIAHPSIADAAVVPQKDDAAGEVPVAFVVRAADS 445
Query: 366 NLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSPAGKILRREL 494
++ E+ + +FV+KQV YK++ +V F AIPKS +GKILR+EL
Sbjct: 444 DIAEEAIKEFVSKQVVFYKRLHKVYFTHAIPKSASGKILRKEL 316
Score = 30.9 bits (61), Expect(2) = 1e-037
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +3 / -3
Query: 126 VDSEGWLKTGXXXYFNEDG 182
+D EGWL TG Y ++DG
Sbjct: 686 IDVEGWLHTGDIGYVDDDG 630
>gb|BF623201.2|BF623201 HVSMEa0015K22f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0015K22f, mRNA sequence
Length = 817
Score = 146 bits (314), Expect(2) = 3e-036
Identities = 60/114 (52%), Positives = 80/114 (70%)
Frame = +3 / +3
Query: 81 IFSGYVGDDEATAATVDSEGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELEH 260
I GY+ D EAT T D +GWL TG Y ++D ++IVDRLKE+IKYKG+QVPPAELE
Sbjct: 417 IMKGYLNDPEATKNTTDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEP 596
Query: 261 ILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYK 422
+L +H +I DAAV+ DE G++P+AFIVR GS ++E ++ F AK+V K
Sbjct: 597 LLITHAEIKDAAVVSMQDELTGEVPVAFIVRIEGSEISESEIKQFGAKEVVSTK 758
Score = 24.9 bits (48), Expect(2) = 3e-036
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +3 / +3
Query: 432 RVAFVSAIPKSPAGK 476
+V F ++PKSPAG+
Sbjct: 765 QVFFADSVPKSPAGR 809
>gb|AL502418.1|AL502418 AL502418 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07I12u 3', mRNA sequence
Length = 592
Score = 121 bits (259), Expect(2) = 2e-033
Identities = 47/79 (59%), Positives = 64/79 (81%)
Frame = +3 / -1
Query: 276 PDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAI 455
P+I+DA VIP+PD++AG++P+A++VR P S+LTE V F+AKQV YK++RRV FV ++
Sbjct: 520 PEILDAVVIPFPDDEAGEVPIAYVVRSPASSLTEVDVQKFIAKQVTYYKRLRRVTFVESV 341
Query: 456 PKSPAGKILRRELVEQAVS 512
PKS AGKILRREL+ Q S
Sbjct: 340 PKSAAGKILRRELIAQVRS 284
Score = 40.5 bits (82), Expect(2) = 2e-033
Identities = 16/24 (66%), Positives = 19/24 (79%)
Frame = +3 / -3
Query: 207 KELIKYKGYQVPPAELEHILNSHP 278
KELIK KG+Q+ PAE E +L SHP
Sbjct: 590 KELIKSKGFQIAPAEXEGLLLSHP 519
>gb|CA007491.1|CA007491 HU08C03r HU Hordeum vulgare subsp. vulgare cDNA clone HU08C03
5-PRIME, mRNA sequence
Length = 581
Score = 141 bits (302), Expect = 5e-033
Identities = 53/88 (60%), Positives = 68/88 (77%)
Frame = +3 / +1
Query: 81 IFSGYVGDDEATAATVDSEGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELEH 260
+ GY + EAT +TV +GWLKTG Y +EDG L++VDRLKELIKYKGYQ+PPAELE
Sbjct: 316 VMKGYFKNTEATQSTVTPDGWLKTGDLCYIDEDGYLFVVDRLKELIKYKGYQLPPAELEA 495
Query: 261 ILNSHPDIMDAAVIPYPDEDAGQLPMAF 344
+L +HP++ D AVIP+PD + GQ PMA+
Sbjct: 496 LLLTHPEVSDVAVIPFPDREVGQFPMAY 579
>gb|CV059902.1|CV059902 BNEL52b8 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL52b8 5' similar to Putative
AMP-binding protein, mRNA sequence
Length = 548
Score = 137 bits (293), Expect = 8e-032
Identities = 53/84 (63%), Positives = 72/84 (85%)
Frame = +3 / +1
Query: 261 ILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVA 440
+L +HP++ D AVIP+PD + GQ PMA++VRK GSNL+ Q+VM+FVAKQVAPYKKVR+VA
Sbjct: 1 LLLTHPEVSDVAVIPFPDREVGQFPMAYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVA 180
Query: 441 FVSAIPKSPAGKILRRELVEQAVS 512
FV+ IPK+ +GKILR++L++ A S
Sbjct: 181 FVTDIPKNASGKILRKDLIKLATS 252
>gb|CV061694.1|CV061694 BNEL70h10 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL70h10 5' similar to Putative
AMP-binding protein, mRNA sequence
Length = 568
Score = 137 bits (293), Expect = 8e-032
Identities = 53/84 (63%), Positives = 72/84 (85%)
Frame = +3 / +1
Query: 261 ILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVA 440
+L +HP++ D AVIP+PD + GQ PMA++VRK GSNL+ Q+VM+FVAKQVAPYKKVR+VA
Sbjct: 1 LLLTHPEVSDVAVIPFPDREVGQFPMAYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVA 180
Query: 441 FVSAIPKSPAGKILRRELVEQAVS 512
FV+ IPK+ +GKILR++L++ A S
Sbjct: 181 FVTDIPKNASGKILRKDLIKLATS 252
>gb|BQ766207.1|BQ766207 EBro08_SQ005_H07_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ005_H07 5', mRNA sequence
Length = 552
Score = 133 bits (286), Expect = 8e-031
Identities = 52/102 (50%), Positives = 74/102 (72%)
Frame = +3 / +3
Query: 81 IFSGYVGDDEATAATVDSEGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELEH 260
I GY+ D E+T T+D +GWL TG ++D ++IVDRLKE+IKYKG+QV PAELE
Sbjct: 240 IMKGYLNDPESTKNTIDKDGWLHTGDIGIVDDDDEIFIVDRLKEIIKYKGFQVAPAELEA 419
Query: 261 ILNSHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQV 386
+L +HP+I DAAV+ D+ AG++P+AF++R GS +TE +
Sbjct: 420 LLITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDI 545
>gb|AL510457.1|AL510457 AL510457 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04P21u 3',
mRNA sequence
Length = 508
Score = 133 bits (284), Expect = 1e-030
Identities = 52/89 (58%), Positives = 69/89 (77%)
Frame = +3 / +1
Query: 81 IFSGYVGDDEATAATVDSEGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELEH 260
I GY+ D ATAAT+D EGWL TG Y ++D ++IVDR+KELIK+KG+QVPPAELE
Sbjct: 232 IMKGYLDDPVATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEA 411
Query: 261 ILNSHPDIMDAAVIPYPDEDAGQLPMAFI 347
+L +HP I DAAV+P D+ AG++P+AF+
Sbjct: 412 LLIAHPSIADAAVVPQKDDAAGEVPVAFV 498
>gb|BI959839.1|BI959839 HVSMEn0021P10f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0021P10f, mRNA sequence
Length = 657
Score = 98.2 bits (208), Expect(2) = 6e-030
Identities = 37/75 (49%), Positives = 57/75 (76%)
Frame = +3 / -1
Query: 270 SHPDIMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVS 449
+HP+I DAAV+ D+ AG++P+AF++R GS +TE + FVAK+V YK++ +V F
Sbjct: 549 THPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDIKKFVAKEVVFYKRIHKVFFTD 370
Query: 450 AIPKSPAGKILRREL 494
+IPK+P+GKILR++L
Sbjct: 369 SIPKNPSGKILRKDL 325
Score = 52.4 bits (108), Expect(2) = 6e-030
Identities = 20/29 (68%), Positives = 26/29 (89%)
Frame = +3 / -3
Query: 171 NEDGLLYIVDRLKELIKYKGYQVPPAELE 257
++D ++IVDRLKE+IKYKG+QV PAELE
Sbjct: 649 DDDDEIFIVDRLKEIIKYKGFQVAPAELE 563
>gb|CA005904.1|CA005904 HU08C03u HU Hordeum vulgare subsp. vulgare cDNA clone HU08C03
3-PRIME, mRNA sequence
Length = 484
Score = 127 bits (272), Expect = 7e-029
Identities = 50/77 (64%), Positives = 66/77 (85%)
Frame = +3 / -1
Query: 282 IMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPK 461
+ D AVIP+PD + GQ PMA++VRK GSNL+ Q+VM+FVAKQVAPYKKVR+VAFV+ IPK
Sbjct: 484 VSDVAVIPFPDREVGQFPMAYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVAFVTDIPK 305
Query: 462 SPAGKILRRELVEQAVS 512
+ +GKILR++L++ A S
Sbjct: 304 NASGKILRKDLIKLATS 254
>gb|CA016130.1|CA016130 HV10D20u HV Hordeum vulgare subsp. vulgare cDNA clone HV10D20
3-PRIME, mRNA sequence
Length = 491
Score = 126 bits (270), Expect = 1e-028
Identities = 50/75 (66%), Positives = 65/75 (86%)
Frame = +3 / -3
Query: 288 DAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSP 467
D AVIP+PD + GQ PMA++VRK GSNL+ Q+VM+FVAKQVAPYKKVR+VAFV+ IPK+
Sbjct: 489 DVAVIPFPDREVGQFPMAYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVAFVTDIPKNA 310
Query: 468 AGKILRRELVEQAVS 512
+GKILR++L++ A S
Sbjct: 309 SGKILRKDLIKLATS 265
>gb|AL510866.1|AL510866 AL510866 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY06D22u 3',
mRNA sequence
Length = 513
Score = 120 bits (257), Expect = 8e-027
Identities = 47/73 (64%), Positives = 63/73 (86%)
Frame = +3 / -1
Query: 294 AVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSPAG 473
A+IP+PD + GQ PM ++VRK GSNL+ Q+VM+FVAKQVAPYKKVR+VAFV+ IPK+ +G
Sbjct: 492 ALIPFPDREVGQFPMXYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVAFVTDIPKNASG 313
Query: 474 KILRRELVEQAVS 512
KILR++L++ A S
Sbjct: 312 KILRKDLIKLATS 274
>gb|AV833853.1|AV833853 AV833853 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags8m01, mRNA sequence
Length = 599
Score = 90.0 bits (190), Expect(2) = 7e-025
Identities = 35/71 (49%), Positives = 53/71 (74%)
Frame = +3 / +3
Query: 282 IMDAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPK 461
I DAAV+ D+ AG++P+AF++R GS +TE + FVAK+V YK++ +V F +IPK
Sbjct: 168 IKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDIKKFVAKEVVFYKRIHKVFFTDSIPK 347
Query: 462 SPAGKILRREL 494
+P+GKILR++L
Sbjct: 348 NPSGKILRKDL 380
Score = 43.7 bits (89), Expect(2) = 7e-025
Identities = 16/21 (76%), Positives = 20/21 (95%)
Frame = +3 / +1
Query: 186 LYIVDRLKELIKYKGYQVPPA 248
++IVDRLKE+IKYKG+QV PA
Sbjct: 70 IFIVDRLKEIIKYKGFQVAPA 132
>gb|BI956935.1|BI956935 HVSMEn0006D04f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0006D04f, mRNA sequence
Length = 654
Score = 106 bits (227), Expect = 1e-022
Identities = 41/89 (46%), Positives = 62/89 (69%)
Frame = +3 / +1
Query: 81 IFSGYVGDDEATAATVDSEGWLKTGXXXYFNEDGLLYIVDRLKELIKYKGYQVPPAELEH 260
I GY+ D ++T T+D +GWL TG ++D ++IVDRLKE+IKYKG+QV PA+LE
Sbjct: 379 IMKGYLNDPKSTKNTIDKDGWLHTGDIGIVDDDDEIFIVDRLKEIIKYKGFQVAPAKLEA 558
Query: 261 ILNSHPDIMDAAVIPYPDEDAGQLPMAFI 347
+L +HP+I D V+ D+ A ++P+ F+
Sbjct: 559 LLITHPEIQDXGVVFLKDDLACEVPIPFV 645
>gb|BQ757736.1|BQ757736 EBem10_SQ005_B05_R embryo, 2 Day germination, no treatment, cv
Optic, EBem10 Hordeum vulgare subsp. vulgare cDNA clone
EBem10_SQ005_B05 5', mRNA sequence
Length = 338
Score = 100 bits (214), Expect = 7e-021
Identities = 42/82 (51%), Positives = 61/82 (74%)
Frame = +3 / +2
Query: 288 DAAVIPYPDEDAGQLPMAFIVRKPGSNLTEQQVMDFVAKQVAPYKKVRRVAFVSAIPKSP 467
+AAV+ Y D+ AG+LP+AF+V++ GS + E Q+ DFVAKQV YK++ RV V +IPK+
Sbjct: 2 EAAVVGYADDQAGELPVAFVVQRSGSKVHEAQIKDFVAKQVVHYKRIHRVFLVDSIPKNA 181
Query: 468 AGKILRRELVEQAVSMGTSKL* 533
AGKILR++L + A+ +KL*
Sbjct: 182 AGKILRKDLAKLALHRINAKL* 247
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 312,232,724
Number of Sequences: 312970
Number of extensions: 4428981
Number of successful extensions: 34375
Number of sequences better than 1.0e-020: 34
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8016758001
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)