TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBS7b05.xg.2.1
(526 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Aven... 187 2e-048
gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Aven... 182 5e-047
gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Aven... 175 6e-045
gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Aven... 122 6e-029
>gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4510_G06_M12, mRNA sequence
Length = 521
Score = 187 bits (402), Expect = 2e-048
Identities = 71/85 (83%), Positives = 80/85 (94%)
Frame = -2 / +2
Query: 489 SDAINNGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRQNRTPTIVVIWSVLLASIFSLL 310
SDA+NNGY SWGPLFGKLFF+FWVIVHLYPFLKGLMGRQNRTPTIVV+WSVLLASIFSL+
Sbjct: 83 SDAVNNGYGSWGPLFGKLFFSFWVIVHLYPFLKGLMGRQNRTPTIVVLWSVLLASIFSLV 262
Query: 309 WVRIDPFIVRTKGPDVRQCGINC*A 235
WVRIDPFI ++KGP ++ CG+ C*A
Sbjct: 263 WVRIDPFIAKSKGPILKPCGVQC*A 337
Score = 148 bits (317), Expect = 8e-037
Identities = 66/85 (77%), Positives = 67/85 (78%)
Frame = +2 / -3
Query: 236 AQQLMPHCLTSGPLVLTMKGSILTQSREKMEASSTDQMTTMVGVRFWRPMSPLRNG*RWT 415
AQ PH L GPL L MKGSILTQ+REKMEAS TDQ TTMVGVRF PMSPLRNG*RWT
Sbjct: 336 AQHCTPHGLRMGPLDLAMKGSILTQTREKMEASRTDQSTTMVGVRFCLPMSPLRNG*RWT 157
Query: 416 MTQKAKKSLPKSGPHDWYPLLMASE 490
MTQK KKS PK GPHD PLL ASE
Sbjct: 156 MTQKEKKSFPKRGPHDP*PLLTASE 82
>gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C09_F17, mRNA
sequence
Length = 676
Score = 182 bits (391), Expect = 5e-047
Identities = 69/84 (82%), Positives = 77/84 (91%)
Frame = -2 / +1
Query: 489 SDAINNGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRQNRTPTIVVIWSVLLASIFSLL 310
S AIN+GYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGR NRTPTIV++W++LLASIFSLL
Sbjct: 76 SYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILLASIFSLL 255
Query: 309 WVRIDPFIVRTKGPDVRQCGINC* 238
WVRIDPF R GP+++ CGINC*
Sbjct: 256 WVRIDPFTTRVAGPNIQTCGINC* 327
Score = 106 bits (227), Expect = 2e-024
Identities = 48/83 (57%), Positives = 56/83 (67%)
Frame = +2 / -3
Query: 242 QLMPHCLTSGPLVLTMKGSILTQSREKMEASSTDQMTTMVGVRFWRPMSPLRNG*RWTMT 421
QL+P GP + GSI T S+EK+EA Q T+VGVRF P+ PLRNG + T+T
Sbjct: 323 QLIPQVWMFGPATRVVNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TIT 144
Query: 422 QKAKKSLPKSGPHDWYPLLMASE 490
QKAKKS P SGPHDW PLLMA E
Sbjct: 143 QKAKKSFPNSGPHDW*PLLMA*E 75
>gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_H12_O23, mRNA
sequence
Length = 620
Score = 175 bits (376), Expect = 6e-045
Identities = 65/79 (82%), Positives = 73/79 (92%)
Frame = -2 / +2
Query: 474 NGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRQNRTPTIVVIWSVLLASIFSLLWVRID 295
+GYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGR NRTPTIV++W++LLASIFSLLWVRID
Sbjct: 2 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILLASIFSLLWVRID 181
Query: 294 PFIVRTKGPDVRQCGINC* 238
PF R GP+++ CGINC*
Sbjct: 182 PFTTRVAGPNIQTCGINC* 238
Score = 102 bits (218), Expect = 4e-023
Identities = 45/78 (57%), Positives = 53/78 (67%)
Frame = +2 / -3
Query: 242 QLMPHCLTSGPLVLTMKGSILTQSREKMEASSTDQMTTMVGVRFWRPMSPLRNG*RWTMT 421
QL+P GP + GSI T S+EK+EA Q T+VGVRF P+ PLRNG + T+T
Sbjct: 234 QLIPQVWMFGPATRVVNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TIT 55
Query: 422 QKAKKSLPKSGPHDWYPL 475
QKAKKS PKSGPHDW PL
Sbjct: 54 QKAKKSFPKSGPHDW*PL 1
>gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4514_A01_A02, mRNA sequence
Length = 474
Score = 122 bits (260), Expect = 6e-029
Identities = 43/60 (71%), Positives = 55/60 (91%)
Frame = -2 / +3
Query: 420 VIVHLYPFLKGLMGRQNRTPTIVVIWSVLLASIFSLLWVRIDPFIVRTKGPDVRQCGINC 241
VIVHLYPFLKGL+GRQNRTPTIV++WS+LLASI SLLWVR++PF+ +T GP + +CG++C
Sbjct: 12 VIVHLYPFLKGLLGRQNRTPTIVIVWSILLASIISLLWVRVNPFLAKTDGPTLEECGLDC 191
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,247,562
Number of Sequences: 8143
Number of extensions: 56066
Number of successful extensions: 1247
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 161763492
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)