TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBL6b07.xg.2.1
(644 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN814886.1|CN814886 HRO4503_E10_J19ZS5 Lib AA071E1X Aven... 127 1e-033
gb|CN816079.1|CN816079 HRO4516_F12_L24ZS5 Lib AA071E1X Aven... 113 2e-029
gb|CN814872.1|CN814872 HRO4503_D07_H13ZS5 Lib AA071E1X Aven... 105 9e-029
gb|CN814884.1|CN814884 HRO4503_E08_J15ZS5 Lib AA071E1X Aven... 64 7e-026
gb|CN818659.1|CN818659 HRO4455_A08_A15ZS5 Lib AA070E1X Aven... 109 1e-024
gb|CN819044.1|CN819044 HRO4474_A04_B08ZS5 Lib AA070E1X Aven... 95 2e-023
gb|CN816738.1|CN816738 HRO4524_C01_F02ZS5 Lib AA071E1X Aven... 94 1e-022
gb|CN816284.1|CN816284 HRO4519_A04_B07ZS5 Lib AA071E1X Aven... 64 2e-022
gb|CN814801.1|CN814801 HRO4502_F05_K10ZS5 Lib AA071E1X Aven... 90 3e-022
>gb|CN814886.1|CN814886 HRO4503_E10_J19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_E10_J19, mRNA sequence
Length = 889
Score = 127 bits (273), Expect(2) = 1e-033
Identities = 63/143 (44%), Positives = 83/143 (58%)
Frame = +2 / +2
Query: 80 AVEIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYK 259
++ I HRDVK++NILLD ++ KV+DFG SRL P + THV T QGT GY+DP Y +
Sbjct: 206 SISIFHRDVKSSNILLDGNYTAKVSDFGASRLVPMDQTHVDTNVQGTFGYLDPEYFHTRQ 385
Query: 260 LTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPELGYET 439
L+EKSDVYSFGVVLVEL+ V G S + +L+N L + +V + E
Sbjct: 386 LSEKSDVYSFGVVLVELLLRMKPVFTGESGTVQSLSNYFLQEFMEGRITSVVKSRVLEEA 565
Query: 440 DGETKKSIDLVAELAFRCLQLER 508
E + +AEL R ER
Sbjct: 566 TEEEIDGVASLAELCLRLHGEER 634
Score = 30.9 bits (61), Expect(2) = 1e-033
Identities = 12/22 (54%), Positives = 17/22 (77%)
Frame = +2 / +2
Query: 17 LTWPRRMSIAIETAEALAYLHA 82
L+W + IA+ETA AL+YLH+
Sbjct: 134 LSWGDYLRIAMETAGALSYLHS 199
>gb|CN816079.1|CN816079 HRO4516_F12_L24ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4516_F12_L24, mRNA sequence
Length = 827
Score = 113 bits (241), Expect(2) = 2e-029
Identities = 49/97 (50%), Positives = 68/97 (70%)
Frame = +2 / +2
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
+IIHRD+K++NILLD F KVADFGL++ THVST GT GY+ P Y KLT
Sbjct: 188 KIIHRDIKSSNILLDFKFEAKVADFGLAKFTSENNTHVSTRVMGTFGYLAPEYASSGKLT 367
Query: 266 EKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMA 376
EKSDV+SFGV+L+EL++ + VD +++ + +L + A
Sbjct: 368 EKSDVFSFGVMLLELITGRRPVDSNQTYMDDSLVDWA 478
Score = 31.3 bits (62), Expect(2) = 2e-029
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +2 / +2
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L WP R+ IA+ A+ LAY+H
Sbjct: 110 LEWPIRLRIALGAAKGLAYIH 172
>gb|CN814872.1|CN814872 HRO4503_D07_H13ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_D07_H13, mRNA sequence
Length = 728
Score = 105 bits (223), Expect(2) = 9e-029
Identities = 45/105 (42%), Positives = 73/105 (69%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTE 268
IIH DVK++NILLD+++ KV+DFG S L P + T+ QGT GY+DP Y Q +LT+
Sbjct: 55 IIHGDVKSSNILLDDNYMAKVSDFGASILAPSDKEQFVTMVQGTCGYLDPEYMQTCQLTD 234
Query: 269 KSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEV 403
KSDVYSFGV+L+E+++ + + + S + +L+ + L+ ++ + +
Sbjct: 235 KSDVYSFGVILLEILTGQLPLKLEGSEKQRSLSLLFLSAMKENNL 369
Score = 37.3 bits (75), Expect(2) = 9e-029
Identities = 16/37 (43%), Positives = 25/37 (67%)
Frame = +2 / +1
Query: 446 ETKKSIDLVAELAFRCLQLERDSRPSMXXVVEALNRI 556
E+ + + +A+LA RCL + D+RPSM V + LNR+
Sbjct: 403 ESMELLGGLADLAKRCLDMCGDNRPSMKEVADELNRL 513
>gb|CN814884.1|CN814884 HRO4503_E08_J15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_E08_J15, mRNA sequence
Length = 830
Score = 64.3 bits (134), Expect(4) = 7e-026
Identities = 33/77 (42%), Positives = 43/77 (55%)
Frame = +2 / +1
Query: 200 STVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMAL 379
ST GT GY P Y +L+ KSDVYSFGVVL+EL++ + VD + +L A
Sbjct: 286 STRVLGTFGYHAPEYAMTGQLSSKSDVYSFGVVLLELLTGRKPVDHTLPRGQQSLVTWAT 465
Query: 380 NRIQNHEVGQLVDPELG 430
R+ +V Q VD LG
Sbjct: 466 PRLSEDKVRQCVDSRLG 516
Score = 39.6 bits (80), Expect(4) = 7e-026
Identities = 16/31 (51%), Positives = 22/31 (70%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYP 181
IIHRD+K++N+LL + K+ADF LS P
Sbjct: 172 IIHRDIKSSNVLLFDDDVAKIADFDLSNQAP 264
Score = 34.5 bits (69), Expect(4) = 7e-026
Identities = 15/36 (41%), Positives = 22/36 (61%)
Frame = +2 / +1
Query: 455 KSIDLVAELAFRCLQLERDSRPSMXXVVEALNRIXN 562
K++ A +A C+Q E D RP+M VV+AL + N
Sbjct: 532 KAVAKFAAVAALCVQYEADFRPNMSIVVKALQPLLN 639
Score = 29.0 bits (57), Expect(4) = 7e-026
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = +2 / +1
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L+W +R+ IA+ A+ L YLH
Sbjct: 91 LSWVQRVKIAVGAAKGLEYLH 153
>gb|CN818659.1|CN818659 HRO4455_A08_A15ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4455_A08_A15, mRNA sequence
Length = 831
Score = 109 bits (232), Expect = 1e-024
Identities = 49/116 (42%), Positives = 71/116 (61%)
Frame = +2 / +3
Query: 83 VEIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKL 262
+ I+HRD+K +NILLD + K++DFGL++L TH+ST GT GY+ P Y L
Sbjct: 27 IRIVHRDIKASNILLDKDMNAKISDFGLAKLNEDGHTHISTKVAGTIGYMAPEYAMRGYL 206
Query: 263 TEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPELG 430
T+K+DVYSFGVV +E+VS K + + L + A + + +LVDP+LG
Sbjct: 207 TDKADVYSFGVVALEIVSGKSNTNYRPKEDFVYLLDWACVLHERGTLLELVDPDLG 374
>gb|CN819044.1|CN819044 HRO4474_A04_B08ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4474_A04_B08, mRNA sequence
Length = 734
Score = 95.0 bits (201), Expect(2) = 2e-023
Identities = 41/72 (56%), Positives = 52/72 (72%)
Frame = +2 / +2
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTE 268
I+H DVK+ NILL +++ KV DFG SR+ P + T+ QGT GY+DP Y Q +LTE
Sbjct: 158 IVHGDVKSPNILLGDNYTAKVTDFGASRMLPKDEIQFMTMVQGTIGYLDPEYLQERQLTE 337
Query: 269 KSDVYSFGVVLV 304
KSDVYSFGVVL+
Sbjct: 338 KSDVYSFGVVLL 373
Score = 29.5 bits (58), Expect(2) = 2e-023
Identities = 12/17 (70%), Positives = 15/17 (88%)
Frame = +2 / +2
Query: 32 RMSIAIETAEALAYLHA 82
R+ IA E+AEALAYLH+
Sbjct: 92 RLRIAQESAEALAYLHS 142
>gb|CN816738.1|CN816738 HRO4524_C01_F02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4524_C01_F02, mRNA sequence
Length = 537
Score = 93.6 bits (198), Expect(2) = 1e-022
Identities = 39/90 (43%), Positives = 59/90 (65%)
Frame = +2 / +2
Query: 86 EIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLT 265
+IIHRDVK N+LLD F V DFGL++L + T V+T +GT G++ P Y K +
Sbjct: 131 KIIHRDVKAANVLLDEYFEPVVGDFGLAKLVDVQKTSVTTQVRGTMGHIAPEYLSTGKSS 310
Query: 266 EKSDVYSFGVVLVELVSSKPAVDMGRSHSE 355
E++DV+ +G++L+ELV+ + A+D R E
Sbjct: 311 ERTDVFGYGIMLLELVTGQRAIDFSRLEEE 400
Score = 28.1 bits (55), Expect(2) = 1e-022
Identities = 11/21 (52%), Positives = 12/21 (57%)
Frame = +2 / +2
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L W R +AI TA L YLH
Sbjct: 53 LDWSARKRVAIGTARGLEYLH 115
>gb|CN816284.1|CN816284 HRO4519_A04_B07ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4519_A04_B07, mRNA sequence
Length = 747
Score = 64.3 bits (134), Expect(3) = 2e-022
Identities = 33/77 (42%), Positives = 43/77 (55%)
Frame = +2 / +1
Query: 200 STVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMAL 379
ST GT GY P Y +L+ KSDVYSFGVVL+EL++ + VD + +L A
Sbjct: 148 STRVLGTFGYHAPEYAMTGQLSSKSDVYSFGVVLLELLTGRKPVDHTLPRGQQSLVTWAT 327
Query: 380 NRIQNHEVGQLVDPELG 430
R+ +V Q VD LG
Sbjct: 328 PRLSEDKVRQCVDSRLG 378
Score = 39.6 bits (80), Expect(3) = 2e-022
Identities = 16/31 (51%), Positives = 22/31 (70%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYP 181
IIHRD+K++N+LL + K+ADF LS P
Sbjct: 34 IIHRDIKSSNVLLFDDDVAKIADFDLSNQAP 126
Score = 34.5 bits (69), Expect(3) = 2e-022
Identities = 15/36 (41%), Positives = 22/36 (61%)
Frame = +2 / +1
Query: 455 KSIDLVAELAFRCLQLERDSRPSMXXVVEALNRIXN 562
K++ A +A C+Q E D RP+M VV+AL + N
Sbjct: 394 KAVAKFAAVAALCVQYEADFRPNMSIVVKALQPLLN 501
>gb|CN814801.1|CN814801 HRO4502_F05_K10ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_F05_K10, mRNA sequence
Length = 848
Score = 89.5 bits (189), Expect(2) = 3e-022
Identities = 44/113 (38%), Positives = 64/113 (56%)
Frame = +2 / +2
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTHVSTVPQGTPGYVDPVYHQCYKLTE 268
IIH D+K NILLD F K+ADFG+++L ++ T +GT GY+ P + + +T
Sbjct: 53 IIHCDIKPENILLDAEFCPKIADFGMAKLLGRDINAALTTLRGTIGYLAPEWVYGHAITH 232
Query: 269 KSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANMALNRIQNHEVGQLVDPEL 427
K+DVYSFGVVL EL+S + RS + A ++ +V L+D L
Sbjct: 233 KADVYSFGVVLFELISGRRTTRSMRSGNHRYFPLYAAAKVNEGDVLCLLDGRL 391
Score = 30.9 bits (61), Expect(2) = 3e-022
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +2 / +2
Query: 455 KSIDLVAELAFRCLQLERDSRPSMXXVVEALNRI 556
K +D++ +A C+Q E RPSM VV L +
Sbjct: 410 KELDVICRVACWCIQDEEIHRPSMGQVVRMLEGV 511
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,365,592
Number of Sequences: 8143
Number of extensions: 86460
Number of successful extensions: 1494
Number of sequences better than 1.0e-020: 9
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 210782732
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)