TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBD3a09.xg.3.1
(1105 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819961.1|CN819961 HRO4409_C01_E01ZS5 Lib AA069E1X Aven... 209 4e-070
>gb|CN819961.1|CN819961 HRO4409_C01_E01ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4409_C01_E01, mRNA
sequence
Length = 540
Score = 209 bits (451), Expect(2) = 4e-070
Identities = 83/94 (88%), Positives = 90/94 (95%)
Frame = +1 / +3
Query: 514 DAIGAIGIARCFTYGGSKNSALHDPRILPRDSLSKEKYMSKEEKQTSINHFHEKLFKLKD 693
D + AIG+ARCFTYGGSKNSALHDP++LPRD+LSKEKYMSKEEKQTSINHFHEKLFKLKD
Sbjct: 96 DRLDAIGVARCFTYGGSKNSALHDPKVLPRDNLSKEKYMSKEEKQTSINHFHEKLFKLKD 275
Query: 694 MMKTEAGKRRAEKRHRFMEEFVAEFYEEWSGRA* 795
MMKTEAGK+RAEKRH FME FV+EFYEEWSGRA*
Sbjct: 276 MMKTEAGKKRAEKRHMFMENFVSEFYEEWSGRA* 377
Score = 137 bits (293), Expect(2) = 2e-049
Identities = 62/90 (68%), Positives = 64/90 (71%)
Frame = -3 / -3
Query: 794 QALPLHXXXXXXXXXXXNLCLFSALLFPASVFIMXXXXXXXXXXXLIDVCFSSLDMYFSF 615
QALPLH N+CLFSAL FPASVFIM LIDVCFSSLDMYFSF
Sbjct: 376 QALPLHSS*NSDTKFSMNMCLFSALFFPASVFIMSFNLKSFSWK*LIDVCFSSLDMYFSF 197
Query: 614 DKLSRGRILGSCNALFLLPPYVKHLAIPIA 525
D+LSRG LGSCNALFLLPPYVKHLA PIA
Sbjct: 196 DRLSRGSTLGSCNALFLLPPYVKHLATPIA 107
Score = 131 bits (280), Expect(2) = 8e-044
Identities = 56/91 (61%), Positives = 64/91 (70%)
Frame = -2 / -2
Query: 798 TSSPATPLFVEFSHKFLHEPMPFLSSPFPGLCLHHVLQFKKLLMKMID*CXXXXXXXXXX 619
TSS ATPL VEF HK LHE +PFL S FP LCLHHVLQ ++LLM+MID*C
Sbjct: 380 TSSSATPLLVEFGHKILHEHVPFLRSLFPSLCLHHVLQLEELLMEMID*CLFLLLGHVLL 201
Query: 618 LRQAITWQNSRIM*CAVLATTICKTSCNTNC 526
L Q I W+ RIM C VLA ++CKTSCNT+C
Sbjct: 200 L*QIIPWKYFRIMQCTVLAPSVCKTSCNTDC 108
Score = 129 bits (277), Expect(2) = 1e-042
Identities = 61/91 (67%), Positives = 70/91 (76%)
Frame = +2 / +1
Query: 527 QLVLQDVLHMVVARTAHYMILEFCHVIACRRKSTCPRKRSRHQSIIFMRSFLN*RT**RQ 706
Q VLQDVLH ART H MIL++ H I C+R+STCPR+R++HQSII MRS +*RT**RQ
Sbjct: 109 QSVLQDVLHTEGARTVHCMILKYFHGIICQRRSTCPRRRNKHQSIISMRSSSS*RT**RQ 288
Query: 707 RPGKGELRKGIGSWRNLWLNSTKSGVAGLEV 799
R GK E RKG SWR L NST+SGVA LEV
Sbjct: 289 RLGKRERRKGTCSWRILCPNSTRSGVAELEV 381
Score = 119 bits (254), Expect(2) = 4e-033
Identities = 59/90 (65%), Positives = 66/90 (73%)
Frame = +3 / +2
Query: 528 NWYCKMFYIWW*QEQRIT*S*NSAT**LVEGKVHVQGREADINQSFS*EAF*TEGHDEDR 707
N CKMFYI QEQ I *S*+++T * V+G+VHVQG E +INQSF *EA EGHDEDR
Sbjct: 110 NRCCKMFYIRREQEQCIA*S*STSTG*SVKGEVHVQGGETNINQSFP*EALQVEGHDEDR 289
Query: 708 GREKES*EKA*VHGGICG*ILRRVEWQGLR 797
G EKES EKA VHG C ILR VEWQ L+
Sbjct: 290 GWEKESGEKAHVHGEFCVRILRGVEWQSLK 379
Score = 95.0 bits (201), Expect(2) = 4e-030
Identities = 47/91 (51%), Positives = 52/91 (57%)
Frame = -1 / -1
Query: 799 NLKPCHSTLRRIQPQIPP*TYAXXXXXXXXXXXXXXXSV*KASHEND*LMSASLPWTCTF 620
+ K CHST RRI+ Q P*T A S *+ASH ND*LM S PWTCT
Sbjct: 381 HFKLCHSTPRRIRTQNSP*TCAFSPLSFSQPLSSSCPST*RASHGND*LMFVSPPWTCTS 202
Query: 619 PSTSYHVAEF*DHVMRCSCYHHM*NILQYQL 527
P T Y V *DH M CSC M*NILQ++L
Sbjct: 201 PLTDYPVEVL*DHAMHCSCSLRM*NILQHRL 109
Score = 74.8 bits (157), Expect(2) = 2e-049
Identities = 31/36 (86%), Positives = 33/36 (91%)
Frame = -3 / -3
Query: 524 PIASRRSASCTIANSRVGSTIDLFETSFLNPIPLII 417
PIASRRSASCTIANSRVGS + FETSFLNPIP+II
Sbjct: 115 PIASRRSASCTIANSRVGSALSFFETSFLNPIPVII 8
Score = 71.2 bits (149), Expect(2) = 4e-070
Identities = 31/37 (83%), Positives = 32/37 (86%)
Frame = +1 / +3
Query: 415 AIIKGMGFKNEVSNKSIVEPTLEFAIVQDADRLDAIG 525
+II GMGFKNEVS K EPTLEFAIVQDADRLDAIG
Sbjct: 6 SIITGMGFKNEVSKKLNAEPTLEFAIVQDADRLDAIG 116
Score = 61.6 bits (128), Expect(2) = 8e-044
Identities = 23/35 (65%), Positives = 27/35 (77%)
Frame = -2 / -2
Query: 522 NCIQAICILYNCKFKSGLNNRFI*NFIFEPHPLNN 418
+CIQ ICIL+NCK KSGL + +*N IFE HP NN
Sbjct: 113 DCIQTICILHNCKLKSGLRIKLL*NLIFESHPSNN 9
Score = 58.8 bits (122), Expect(2) = 1e-042
Identities = 23/35 (65%), Positives = 26/35 (74%)
Frame = +3 / +2
Query: 417 NY*GDGVQK*SFK*IYC*AHS*ICNCTRCRSPGCN 521
+Y DG+QK* FK C AHS*+CNC RCRS GCN
Sbjct: 8 DYYWDGIQK*GFKEA*CGAHS*VCNCARCRSSGCN 112
Score = 51.9 bits (107), Expect(2) = 4e-030
Identities = 22/32 (68%), Positives = 23/32 (71%)
Frame = -1 / -1
Query: 523 QLHPGDLHLVQLQIQEWAQQ*IYLKLHF*TPS 428
+LHP DLHL QLQ QEWA * LK HF* PS
Sbjct: 114 RLHPDDLHLAQLQTQEWAPH*ASLKPHF*IPS 19
Score = 37.7 bits (76), Expect(2) = 4e-033
Identities = 16/35 (45%), Positives = 19/35 (54%)
Frame = +2 / +1
Query: 416 QLLRGWGSKMKFQINXXXXXXXXXXXYKMQIAWMQ 520
+LL GW SKM+FQ + KMQI WMQ
Sbjct: 7 RLLLGWDSKMRFQRSLMRSPLLSLQLCKMQIVWMQ 111
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,081,747
Number of Sequences: 8143
Number of extensions: 171742
Number of successful extensions: 1175
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 390569985
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)