TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAY3d02.yg.2.1
(541 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF082991.1|AF082991 Avena sativa beta-D-glucosidase beta... 126 5e-051
emb|X78433.1|ASLBDG A.sativa L. mRNA for beta-D-glucosidase 113 1e-046
>gb|AF082991.1|AF082991 Avena sativa beta-D-glucosidase beta subunit precursor (P60b) mRNA,
complete cds
Length = 1923
Score = 126 bits (270), Expect(3) = 5e-051
Identities = 43/97 (44%), Positives = 69/97 (71%)
Frame = +1 / +1
Query: 145 GGVNQAGVKFYNSLINGLLEKGIQPFVTINHYDIPEELQERYNSWLNLEIQEDFTYFAEL 324
GG+N G+++YN L++ L+E GI+P++T+ H+D P+ L ++YN +L+ I +D+T +A +
Sbjct: 493 GGINHEGIQYYNDLLDCLIENGIKPYITLFHWDTPQALADKYNDFLDRRIVKDYTDYATV 672
Query: 325 CFKXFGDRVKHWVTFNEPNLIXKLAYSXXAFPPNXCS 435
CF+ FGD+VK+W+TFNEP+ LAY P CS
Sbjct: 673 CFEHFGDKVKNWITFNEPHSFCGLAYGTGLHAPGLCS 783
Score = 75.8 bits (159), Expect(3) = 5e-051
Identities = 29/49 (59%), Positives = 37/49 (75%)
Frame = +1 / +1
Query: 4 IDDGSNGDVAADHYHRYKDDIEMMHSIGLGSYRFSLSWSRILPKGRFGG 150
I DGSN DVAA+ Y+ YK+D+ M+ IG+ SYRFS+SW RILP+G G
Sbjct: 349 IMDGSNWDVAANSYYMYKEDVRMLKEIGMDSYRFSISWPRILPEGTLEG 495
Score = 31.8 bits (63), Expect(3) = 5e-051
Identities = 10/26 (38%), Positives = 18/26 (69%)
Frame = +1 / +1
Query: 460 GNSSTXXYIXAHNMILAHAKXXNIYR 537
G++ YI HN++LAHA+ ++Y+
Sbjct: 811 GDALRQPYIVGHNLLLAHAETVDVYK 888
>emb|X78433.1|ASLBDG A.sativa L. mRNA for beta-D-glucosidase
Length = 1898
Score = 113 bits (242), Expect(3) = 1e-046
Identities = 39/93 (41%), Positives = 63/93 (67%)
Frame = +1 / +1
Query: 145 GGVNQAGVKFYNSLINGLLEKGIQPFVTINHYDIPEELQERYNSWLNLEIQEDFTYFAEL 324
GG+N G+++YN L++ L+E GI+P++T+ H+D P+ L + Y +L+ I +D+T +A +
Sbjct: 487 GGINHEGIQYYNDLLDCLIENGIKPYITLFHWDTPQALADEYKDFLDRRIVKDYTDYATV 666
Query: 325 CFKXFGDRVKHWVTFNEPNLIXKLAYSXXAFPP 423
CF+ FGD+VK+W TFNEP+ L Y P
Sbjct: 667 CFEHFGDKVKNWFTFNEPHSFCGLGYGTGLHAP 765
Score = 75.3 bits (158), Expect(3) = 1e-046
Identities = 29/49 (59%), Positives = 36/49 (73%)
Frame = +1 / +1
Query: 4 IDDGSNGDVAADHYHRYKDDIEMMHSIGLGSYRFSLSWSRILPKGRFGG 150
I D SN DVAA+ Y+ YK+D+ M+ IG+ SYRFS+SW RILPKG G
Sbjct: 343 IMDKSNADVAANSYYMYKEDVRMLKEIGMDSYRFSISWPRILPKGTLDG 489
Score = 29.9 bits (59), Expect(3) = 1e-046
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = +1 / +1
Query: 481 YIXAHNMILAHAKXXNIY 534
YI HN++LAHA+ ++Y
Sbjct: 829 YIVGHNLLLAHAETVDVY 882
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,041,390
Number of Sequences: 8143
Number of extensions: 53111
Number of successful extensions: 846
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 167890897
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)