TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF25f04.yg.3.3
(1599 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818871.1|CN818871 HRO4456_A03_A06ZS5 Lib AA070E1X Aven... 90 5e-028
>gb|CN818871.1|CN818871 HRO4456_A03_A06ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4456_A03_A06, mRNA sequence
Length = 609
Score = 90.4 bits (191), Expect(4) = 5e-028
Identities = 37/57 (64%), Positives = 42/57 (73%)
Frame = -3 / -3
Query: 1207 TRNTTNLPSRLAIR*GQSAPEWQAFVGAPFLFGQQPLPRIDPCYPELRTPSTAPGNL 1037
+R+T NLPSRLAI GQ A EW + V PFLF QQPLP DPCYP +TPS APG+L
Sbjct: 181 SRHTMNLPSRLAIHSGQ*AQEWTSSVAVPFLFVQQPLPHTDPCYPAPKTPSIAPGSL 11
Score = 84.0 bits (177), Expect(3) = 3e-024
Identities = 37/56 (66%), Positives = 40/56 (71%)
Frame = +2 / +2
Query: 1037 EISWCSRRSSQLRITRIYTRKRLLPKEKGSANECLPFRG*LPLANCKTRW*IRSVP 1204
E SWC+RRS + RITRI RKRLL KEK + N PF G LP NCKTRW*I SVP
Sbjct: 11 ETSWCNRRSFRCRITRICMRKRLLYKEKRNGNG*CPFLGSLPRVNCKTRW*IHSVP 178
Score = 82.2 bits (173), Expect(2) = 4e-021
Identities = 37/56 (66%), Positives = 39/56 (69%)
Frame = +1 / +1
Query: 1030 HIRDFLVQSKEFSAQDNKDLYXXXXXXXXXXXXXXMLAIPGLIAPSELQDEMVDS* 1197
H DFLVQSKEFS QDNKDLY M++IPGLIAPSELQDEMVDS*
Sbjct: 4 HEGDFLVQSKEFSVQDNKDLYAEEAAVQREKERQRMMSIPGLIAPSELQDEMVDS* 171
Score = 44.1 bits (90), Expect(4) = 5e-028
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = -2 / -1
Query: 1343 SCGGNQK*NYSSHLSPPWKKTR 1278
S GGN++*NYS HLSPPWK T+
Sbjct: 306 SSGGNRE*NYSRHLSPPWKITK 241
Score = 40.0 bits (81), Expect(3) = 3e-024
Identities = 15/21 (71%), Positives = 16/21 (76%)
Frame = +2 / +3
Query: 1286 SSRVVKGGCCNFIFGYLHMML 1348
SS VVKG CCNFI G LH+ L
Sbjct: 249 SSMVVKGACCNFILGCLHLTL 311
Score = 35.0 bits (70), Expect(2) = 4e-021
Identities = 19/33 (57%), Positives = 19/33 (57%)
Frame = +3 / +1
Query: 1245 WSGLGSPRLSSASFLPGW*KVAAVILFLVTST* 1343
W G S S LP W*KV AVILF V ST*
Sbjct: 208 WRGPRHHPPKSLSDLPWW*KVPAVILFSVAST* 306
Score = 26.3 bits (51), Expect(3) = 3e-024
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3 / +1
Query: 1554 SRLIGTLIYLYCTIL 1598
SRLIGTL YCT++
Sbjct: 550 SRLIGTL*SCYCTVI 594
Score = 24.4 bits (47), Expect(4) = 5e-028
Identities = 8/15 (53%), Positives = 10/15 (66%)
Frame = -3 / -2
Query: 1522 QCCTTMIYHGQSTRT 1478
+CCT +I HG S T
Sbjct: 485 KCCTVIINHGSSHST 441
Score = 22.6 bits (43), Expect(4) = 5e-028
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -1 / -3
Query: 1590 YSTDISRFLSAWI 1552
Y++ I RFLS WI
Sbjct: 586 YNSRIIRFLSVWI 548
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 19,273,903
Number of Sequences: 8143
Number of extensions: 292519
Number of successful extensions: 1320
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 584912324
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)