TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAD12g05.sg.3.2
(900 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815889.1|CN815889 HRO4511_F03_L05ZS5 Lib AA071E1X Aven... 310 2e-085
gb|CN815830.1|CN815830 HRO4510_H11_O22ZS5 Lib AA071E1X Aven... 271 9e-074
gb|CN817669.1|CN817669 HRO4453_H03_P05ZS5 Lib AA070E1X Aven... 162 8e-041
>gb|CN815889.1|CN815889 HRO4511_F03_L05ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4511_F03_L05, mRNA sequence
Length = 744
Score = 310 bits (671), Expect = 2e-085
Identities = 125/149 (83%), Positives = 132/149 (88%)
Frame = +1 / -2
Query: 316 MIEGPFVFTPDFFKKENPNFFDEVWKEYSKGDSRYVARDTATVTVEGITAVLEGPASLLA 495
+IEGPFVFTPDFF K +PNFFDE+WKEYSKGDSRYVARDTATVTVEGITAVLEGPASLLA
Sbjct: 617 LIEGPFVFTPDFFSKNSPNFFDELWKEYSKGDSRYVARDTATVTVEGITAVLEGPASLLA 438
Query: 496 VYAIASRKSFSHILQFAVCLGQLYGCLVYFITAYLDGFNFWVGPFYFWAYFIGANSFWIW 675
VYAIA+ KS+SHILQF VCLGQLYGCLVYFITAYLDGFNFWV PFYFWAYFIGANS W+
Sbjct: 437 VYAIAAGKSYSHILQFTVCLGQLYGCLVYFITAYLDGFNFWVSPFYFWAYFIGANSSWVV 258
Query: 676 IPMLIAIRSWKKTCAAFQAEKVKKTK*KL 762
IP LIA RSWKK CAA + KTK* L
Sbjct: 257 IPTLIAARSWKKICAAIHQSEKVKTK*GL 171
Score = 213 bits (459), Expect = 4e-056
Identities = 102/146 (69%), Positives = 112/146 (76%)
Frame = -2 / +1
Query: 755 YLVFFTFSA*NAAQVFFQDLMAMSIGIQIQKLFAPMKYAQK*NGPTQKLKPSKYAVMK*T 576
YLVF * AAQ+FFQ L A+S+GI +LFAPMKYAQK*NG TQKL PSK AVMK*T
Sbjct: 178 YLVFTFSL**IAAQIFFQLLAAISVGITTHELFAPMKYAQK*NGLTQKLNPSK*AVMK*T 357
Query: 575 RHP*SWPRQTANWRMWLKDFLDAIA*TASSDAGPSNTAVIPSTVTVAVSLATYLESPLLY 396
+HP*S P+ T NWR+WL DF AIA*TAS +AGPSNTAVIPSTVTVAVSLATYLESPLLY
Sbjct: 358 KHP*S*PKHTVNWRIWL*DFPAAIA*TASREAGPSNTAVIPSTVTVAVSLATYLESPLLY 537
Query: 395 SFQTXXXXXXXXXXXXXGVKTKGPSI 318
SFQ+ GVKTKGPS+
Sbjct: 538 SFQSSSKKLGLFLEKKSGVKTKGPSM 615
Score = 176 bits (379), Expect = 4e-045
Identities = 75/133 (56%), Positives = 92/133 (69%)
Frame = -1 / +3
Query: 717 ASFLPGPYGDEHRYPDPEAVCANEIRPEVERADPEVEAVQVRGDEVNQASVELAQTDGEL 538
A LP P GD+ Y +P VCA+EI PEVER DPEVE +QV GDEV+QASVEL Q EL
Sbjct: 216 ADLLPAPRGDQRWYHNPRTVCADEICPEVERTDPEVEPIQVSGDEVDQASVELTQAYSEL 395
Query: 537 ENVAEGLPGCNSVDSK**CRAFQYSGDPFDRNSCSVPSNIPRVSLTILFPNFIKEIGVLL 358
E++A G P CN +D K RAFQ+S D FD + VP ++ V+ +L P FI+EIG +L
Sbjct: 396 EDMAVGFPSCNRIDCKQGSRAFQHSRDSFDGDGGGVPGDVSGVAFAVLLPEFIEEIGAVL 575
Query: 357 LEEIRSEDKGPLD 319
EEIR ED+GPLD
Sbjct: 576 GEEIRGEDEGPLD 614
Score = 86.8 bits (183), Expect(2) = 8e-025
Identities = 42/84 (50%), Positives = 53/84 (63%)
Frame = +3 / -1
Query: 321 RGALCLHS*FLQEGEPQFL**SLERV**GRL*VCC*GHCNCYGRRDHRCIGRPCITTCCL 500
RGAL LH FL + +PQFL *+LE V RL + GH + + RR+H C+GRPC C L
Sbjct: 612 RGALRLHPGFLLQEQPQFLR*TLEGVQQRRLQIRRQGHRHRHRRRNHGCVGRPCFPACSL 433
Query: 501 RYCIQEVLQPHSPVRRLSGPALRM 572
C E+LQP+ PV + G ALRM
Sbjct: 432 CDCSWEILQPYPPVHCMLGSALRM 361
Score = 41.8 bits (85), Expect(2) = 8e-025
Identities = 19/38 (50%), Positives = 25/38 (65%)
Frame = +3 / -1
Query: 645 FHWRKQLLDLDTDAHRHKVLEENLRRVSS*EGEEDQIK 758
FH RKQ + DT+A R + LEE+LR SS EDQ++
Sbjct: 288 FHRRKQFVGCDTNADRREELEEDLRSNSSKRKGEDQVR 175
>gb|CN815830.1|CN815830 HRO4510_H11_O22ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4510_H11_O22, mRNA sequence
Length = 677
Score = 271 bits (587), Expect = 9e-074
Identities = 111/132 (84%), Positives = 115/132 (87%)
Frame = +1 / +3
Query: 367 PNFFDEVWKEYSKGDSRYVARDTATVTVEGITAVLEGPASLLAVYAIASRKSFSHILQFA 546
PNFFD VWKEYSKGDSRYVARDTATVTVEGITAVLEGPASLLAVYAIAS KS+SHILQF
Sbjct: 3 PNFFDVVWKEYSKGDSRYVARDTATVTVEGITAVLEGPASLLAVYAIASGKSYSHILQFT 182
Query: 547 VCLGQLYGCLVYFITAYLDGFNFWVGPFYFWAYFIGANSFWIWIPMLIAIRSWKKTCAAF 726
VCLGQLYGCLVYFITAYLDGFNFW PFYFWAYFIGANS W+ IP LIA RSWK+ CAA
Sbjct: 183 VCLGQLYGCLVYFITAYLDGFNFWASPFYFWAYFIGANSSWVVIPTLIAARSWKRICAAI 362
Query: 727 QAEKVKKTK*KL 762
+ KTK* L
Sbjct: 363 HQSEKVKTK*GL 398
Score = 198 bits (427), Expect = 1e-051
Identities = 89/113 (78%), Positives = 97/113 (85%)
Frame = -2 / -2
Query: 722 AAQVFFQDLMAMSIGIQIQKLFAPMKYAQK*NGPTQKLKPSKYAVMK*TRHP*SWPRQTA 543
AAQ+ FQ L A+S+GI +LFAPMKYAQK*NG QKLKPSK AVMK T+HP*S PR T
Sbjct: 358 AAQILFQLLAAISVGITTHELFAPMKYAQK*NGLAQKLKPSK*AVMKYTKHP*S*PRHTV 179
Query: 542 NWRMWLKDFLDAIA*TASSDAGPSNTAVIPSTVTVAVSLATYLESPLLYSFQT 384
NWR+WL DF DA+A*TAS +AGPSNTAVIPSTVTVAVSLATYLESPLLYSFQT
Sbjct: 178 NWRIWL*DFPDAMA*TASREAGPSNTAVIPSTVTVAVSLATYLESPLLYSFQT 20
Score = 137 bits (294), Expect = 2e-033
Identities = 61/114 (53%), Positives = 78/114 (68%)
Frame = -1 / -1
Query: 708 LPGPYGDEHRYPDPEAVCANEIRPEVERADPEVEAVQVRGDEVNQASVELAQTDGELENV 529
LP P G++ Y +P AVCA+EI PEVER PEVEA+QV GDEV QASVEL + ELE++
Sbjct: 344 LPAPCGNQCWYHNP*AVCADEICPEVERTGPEVEAIQVGGDEVYQASVELTEAHRELEDM 165
Query: 528 AEGLPGCNSVDSK**CRAFQYSGDPFDRNSCSVPSNIPRVSLTILFPNFIKEIG 367
A GLP CN +D K RAFQ+ D FD + VP ++ V+ +L P+ I+EIG
Sbjct: 164 AVGLPRCNGIDCKQGSRAFQHGRDSFDGDGGGVPGDVSGVAFAVLLPDDIEEIG 3
>gb|CN817669.1|CN817669 HRO4453_H03_P05ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4453_H03_P05, mRNA sequence
Length = 433
Score = 162 bits (348), Expect = 8e-041
Identities = 63/82 (76%), Positives = 68/82 (82%)
Frame = +1 / +3
Query: 517 KSFSHILQFAVCLGQLYGCLVYFITAYLDGFNFWVGPFYFWAYFIGANSFWIWIPMLIAI 696
+++SHILQF VCLGQLYGCLVYFITAYLDGFNFWV PFYFWAYFIGANS W+ IP LIA
Sbjct: 9 EAYSHILQFTVCLGQLYGCLVYFITAYLDGFNFWVSPFYFWAYFIGANSSWVVIPTLIAA 188
Query: 697 RSWKKTCAAFQAEKVKKTK*KL 762
RSWKK CAA + KTK* L
Sbjct: 189 RSWKKICAAIHQSEKVKTK*GL 254
Score = 116 bits (249), Expect = 4e-027
Identities = 54/77 (70%), Positives = 60/77 (77%)
Frame = -2 / -1
Query: 755 YLVFFTFSA*NAAQVFFQDLMAMSIGIQIQKLFAPMKYAQK*NGPTQKLKPSKYAVMK*T 576
YLVF * AAQ+FFQ L A+S+GI +LFAPMKYAQK* G TQKLKPSK AVMK*T
Sbjct: 247 YLVFTFSL**IAAQIFFQLLAAISVGITTHELFAPMKYAQK*KGLTQKLKPSK*AVMK*T 68
Query: 575 RHP*SWPRQTANWRMWL 525
+HP*S PR T NWR+WL
Sbjct: 67 KHP*S*PRHTVNWRIWL 17
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,369,323
Number of Sequences: 8143
Number of extensions: 123949
Number of successful extensions: 944
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 311638528
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)