TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8636641.2.1
(1326 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF078871.1|AF078871 Avena sativa cultivar Ogle peroxidas... 90 7e-049
gb|AF078872.1|AF078872 Avena sativa cultivar Ogle peroxidas... 90 9e-049
gb|CN816630.1|CN816630 HRO4523_A06_B11ZS5 Lib AA071E1X Aven... 80 1e-027
>gb|AF078871.1|AF078871 Avena sativa cultivar Ogle peroxidase PXC2 precursor (pxc2) gene,
complete cds
Length = 1191
Score = 90.4 bits (191), Expect(4) = 7e-049
Identities = 44/114 (38%), Positives = 59/114 (51%)
Frame = +2 / +1
Query: 665 GIDPTLSKAYAFLLKSICPANTSQFFPNTTVFMDLITPERFDNK*YVGLTNNLGLFKSDV 844
G D ++ A+A L++ CP T ++ +D TP FDN Y L + GL SD
Sbjct: 841 GGDTNINAAFATSLQANCPQATGGSGDSSLAPLDTKTPNAFDNSYYNNLLSQKGLLHSDQ 1020
Query: 845 ALLTNATMKALVDSFVRSEATFRTKFARSMIKMGQIEVLTGTQGEIRRNCRVIN 1006
L N T V +F S + F F +MIKMG I LTGTQG+IR +C +N
Sbjct: 1021 VLFNNGTTDNTVRNFASSASAFTGAFTTAMIKMGNISPLTGTQGQIRLSCSKVN 1182
Score = 61.6 bits (128), Expect(4) = 7e-049
Identities = 27/47 (57%), Positives = 32/47 (68%)
Frame = +2 / +3
Query: 257 EKDAPPNNPSLRFFDVVDRAKASLEAQCPGVVSCADVLAFAARDSVV 397
E++A PN SLR F V+D K +EA C VSC D+LA AARDSVV
Sbjct: 372 EQNAAPNAGSLRGFSVIDNIKTQVEAVCKQTVSCDDILAVAARDSVV 512
Score = 53.3 bits (110), Expect(4) = 7e-049
Identities = 19/41 (46%), Positives = 28/41 (68%)
Frame = +2 / +1
Query: 83 FYDRTCPTAETIVQQTVAAAFRNNSGVAPALIRMHFHDCFV 205
FYD +CP A ++ VAAA ++ + +L+R+HFHDCFV
Sbjct: 88 FYDTSCPKALATIKSGVAAAVSSDRRMGASLLRLHFHDCFV 210
Score = 49.6 bits (102), Expect(4) = 7e-049
Identities = 20/43 (46%), Positives = 26/43 (60%)
Frame = +2 / +1
Query: 476 NLPPPFFNATELADRFASKNLTIEDLVVLSGAHTIGVSHCSGF 604
+LP P + +L F+ KNL D+V LSGAHTIG + C F
Sbjct: 697 DLPAPTSSLAQLQAAFSKKNLDTTDMVALSGAHTIGQAQCKNF 825
Score = 49.6 bits (102), Expect(4) = 2e-022
Identities = 33/102 (32%), Positives = 47/102 (46%)
Frame = -3 / -1
Query: 994 AVAPDLALRPRQHLDLPHLDHGPGKLGPESRLAAHEGVDQGLHRRVGQQRHV*LEEAQVV 815
A PDLALRP Q D+ HLDHG G+ E R A E D + V +Q V ++++ +
Sbjct: 1170 AAEPDLALRPGQRRDVSHLDHGGGEGAGERRRAGREVPDGVVGGAVVEQHLVRVQKSLLG 991
Query: 814 GQADVLLVVKPFXXXXXXXXXXXXXELAGVGRADALEKKCVG 689
+ V+ VV+ AG A LE++ G
Sbjct: 990 QEIVVVAVVERVGGLRVQRRQAAVAGAAGRLGAVGLERRREG 865
Score = 46.4 bits (95), Expect(4) = 2e-022
Identities = 21/42 (50%), Positives = 24/42 (57%)
Frame = -1 / -2
Query: 603 KPLQCETPMVCAPESTTRSSIVRFLEANLSASSVALKNGGGR 478
K L C PMVCAPES T S + RF N + S L+ G GR
Sbjct: 824 KFLHCACPMVCAPESATMSVVSRFFLLNAAWSCARLEVGAGR 699
Score = 41.4 bits (84), Expect(4) = 2e-022
Identities = 20/41 (48%), Positives = 23/41 (56%)
Frame = -1 / -2
Query: 207 LTKQSWKCMRISAGATPELFLNAAATVCCTMVSAVGHVLS* 85
LTKQSWKC R S L AAAT + A+GH +S*
Sbjct: 212 LTKQSWKCSRSSDAPMRRSLLTAAATPLLMVARALGHDVS* 90
Score = 26.7 bits (52), Expect(4) = 2e-022
Identities = 19/52 (36%), Positives = 22/52 (42%)
Frame = -3 / -2
Query: 412 EATGEHDAVPGREGEHVGAGDHAGALSLQ*RLGTVDHVEEPEAGVVGWRVLL 257
+A D V G +GE V A L L VD E P+ V RVLL
Sbjct: 527 DAAYRDDGVTGGDGEDVVT*HSLLAHGLHLGLDVVDDTEAPQRPRVRCRVLL 372
>gb|AF078872.1|AF078872 Avena sativa cultivar Ogle peroxidase PXC6 precursor (pxc6) gene,
complete cds
Length = 1197
Score = 90.4 bits (191), Expect(4) = 9e-049
Identities = 44/114 (38%), Positives = 59/114 (51%)
Frame = +2 / +1
Query: 665 GIDPTLSKAYAFLLKSICPANTSQFFPNTTVFMDLITPERFDNK*YVGLTNNLGLFKSDV 844
G D ++ A+A L++ CP T ++ +D TP FDN Y L + GL SD
Sbjct: 847 GGDTNINAAFATSLQANCPQATGGSGDSSLAPLDTKTPNAFDNSYYNNLLSQKGLLHSDQ 1026
Query: 845 ALLTNATMKALVDSFVRSEATFRTKFARSMIKMGQIEVLTGTQGEIRRNCRVIN 1006
L N T V +F S + F F +MIKMG I LTGTQG+IR +C +N
Sbjct: 1027 VLFNNGTTDNTVRNFASSASAFTGAFTTAMIKMGNISPLTGTQGQIRLSCSKVN 1188
Score = 64.3 bits (134), Expect(4) = 9e-049
Identities = 28/47 (59%), Positives = 33/47 (70%)
Frame = +2 / +3
Query: 257 EKDAPPNNPSLRFFDVVDRAKASLEAQCPGVVSCADVLAFAARDSVV 397
E++A PN SLR F V+D K +EA C VSCAD+LA AARDSVV
Sbjct: 372 EQNAAPNAGSLRGFSVIDNIKTQVEAVCKQTVSCADILAVAARDSVV 512
Score = 53.3 bits (110), Expect(4) = 9e-049
Identities = 19/41 (46%), Positives = 28/41 (68%)
Frame = +2 / +1
Query: 83 FYDRTCPTAETIVQQTVAAAFRNNSGVAPALIRMHFHDCFV 205
FYD +CP A ++ VAAA ++ + +L+R+HFHDCFV
Sbjct: 88 FYDTSCPKALATIKSGVAAAVSSDRRMGASLLRLHFHDCFV 210
Score = 49.6 bits (102), Expect(4) = 6e-023
Identities = 33/102 (32%), Positives = 47/102 (46%)
Frame = -3 / -1
Query: 994 AVAPDLALRPRQHLDLPHLDHGPGKLGPESRLAAHEGVDQGLHRRVGQQRHV*LEEAQVV 815
A PDLALRP Q D+ HLDHG G+ E R A E D + V +Q V ++++ +
Sbjct: 1176 AAEPDLALRPGQRRDVSHLDHGGGEGAGERRRAGREVPDGVVGGAVVEQHLVRVQKSLLG 997
Query: 814 GQADVLLVVKPFXXXXXXXXXXXXXELAGVGRADALEKKCVG 689
+ V+ VV+ AG A LE++ G
Sbjct: 996 QEIVVVAVVERVGGLRVQRRQAAVAGAAGRLGAVGLERRREG 871
Score = 46.4 bits (95), Expect(4) = 9e-049
Identities = 19/43 (44%), Positives = 25/43 (58%)
Frame = +2 / +1
Query: 476 NLPPPFFNATELADRFASKNLTIEDLVVLSGAHTIGVSHCSGF 604
+LP P + +L F+ KNL +V LSGAHTIG + C F
Sbjct: 703 DLPAPTSSLAQLQAAFSKKNLDTTGMVALSGAHTIGQAQCKNF 831
Score = 44.1 bits (90), Expect(4) = 6e-023
Identities = 20/42 (47%), Positives = 23/42 (54%)
Frame = -1 / -2
Query: 603 KPLQCETPMVCAPESTTRSSIVRFLEANLSASSVALKNGGGR 478
K L C PMVCAPES T + RF N + S L+ G GR
Sbjct: 830 KFLHCACPMVCAPESATMPVVSRFFLLNAAWSCARLEVGAGR 705
Score = 41.4 bits (84), Expect(4) = 6e-023
Identities = 20/41 (48%), Positives = 23/41 (56%)
Frame = -1 / -2
Query: 207 LTKQSWKCMRISAGATPELFLNAAATVCCTMVSAVGHVLS* 85
LTKQSWKC R S L AAAT + A+GH +S*
Sbjct: 212 LTKQSWKCSRSSDAPMRRSLLTAAATPLLMVARALGHDVS* 90
Score = 30.9 bits (61), Expect(4) = 6e-023
Identities = 20/52 (38%), Positives = 23/52 (44%)
Frame = -3 / -2
Query: 412 EATGEHDAVPGREGEHVGAGDHAGALSLQ*RLGTVDHVEEPEAGVVGWRVLL 257
+A D V G +GE VG A L L VD E P+ V RVLL
Sbjct: 527 DAAYRDDGVTGGDGEDVGT*HSLLAHGLHLGLDVVDDAEAPQRPRVRCRVLL 372
>gb|CN816630.1|CN816630 HRO4523_A06_B11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4523_A06_B11, mRNA sequence
Length = 737
Score = 80.3 bits (169), Expect(2) = 1e-027
Identities = 35/82 (42%), Positives = 45/82 (54%)
Frame = +2 / +3
Query: 761 MDLITPERFDNK*YVGLTNNLGLFKSDVALLTNATMKALVDSFVRSEATFRTKFARSMIK 940
+D +TP FDN Y L + GL SD L + + V SF S +TF + FA +M+
Sbjct: 276 LDTMTPNAFDNAYYTNLQSQKGLLHSDQELFNGGSADSTVSSFASSASTFSSAFATAMVN 455
Query: 941 MGQIEVLTGTQGEIRRNCRVIN 1006
MG I TGTQGEIR C +N
Sbjct: 456 MGNIAPKTGTQGEIRLTCSKVN 521
Score = 61.6 bits (128), Expect(2) = 1e-027
Identities = 24/54 (44%), Positives = 35/54 (64%)
Frame = +2 / +3
Query: 443 GRISNDTEALNNLPPPFFNATELADRFASKNLTIEDLVVLSGAHTIGVSHCSGF 604
G ++ + A +NLP P F+ L D F++K L++ D++ LSGAHTIG S C F
Sbjct: 9 GTTASKSNAESNLPAPTFSLQNLTDNFSNKGLSLTDMIALSGAHTIGQSQCRFF 170
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,626,342
Number of Sequences: 8143
Number of extensions: 181772
Number of successful extensions: 1565
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 480050415
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)