TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3829517.2.1
(913 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816947.1|CN816947 HRO4526_E12_I24ZS5 Lib AA071E1X Aven... 114 3e-069
gb|CN817117.1|CN817117 HRO4528_D08_H16ZS5 Lib AA071E1X Aven... 114 3e-069
gb|CN817427.1|CN817427 HRO4465_G11_N21ZS5 Lib AA070E1X Aven... 110 1e-047
gb|CN818645.1|CN818645 HRO4467_E04_I07ZS5 Lib AA070E1X Aven... 133 4e-032
>gb|CN816947.1|CN816947 HRO4526_E12_I24ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4526_E12_I24, mRNA sequence
Length = 867
Score = 114 bits (243), Expect(4) = 3e-069
Identities = 47/69 (68%), Positives = 52/69 (75%)
Frame = +1 / +3
Query: 463 LKGCAGKYATGDEVQLADVFLAPQIYAAIERTKIDMSNYPTLARLHSEYMSHPAFEAALP 642
L GC KY+ GDEV L DVFLAPQI+AAI R IDM+ YPTLAR H YM PAF+AALP
Sbjct: 453 LDGCDSKYSIGDEVHLGDVFLAPQIHAAIHRFHIDMTKYPTLARFHDAYMEIPAFQAALP 632
Query: 643 GKQPDAPSS 669
QPDAPS+
Sbjct: 633 QNQPDAPST 659
Score = 108 bits (230), Expect(4) = 3e-069
Identities = 44/75 (58%), Positives = 59/75 (78%)
Frame = +1 / +3
Query: 142 QSDPEFVKLNPMKFVPALVDGSSVIGDSYAITLYLEDKYPEPPLLPQDLQKKALNHQIAS 321
++D ++ K+NP+K++PALVD V+ DS AI LYLEDKYP+ PLLP+DL+KKAL+ QIA+
Sbjct: 141 RTDTDYEKINPIKYIPALVDEDFVVSDSLAIILYLEDKYPQHPLLPKDLKKKALDLQIAN 320
Query: 322 IVASGIQPLHNLTVL 366
IV S IQPL V+
Sbjct: 321 IVCSSIQPLQGYGVI 365
Score = 79.0 bits (166), Expect(3) = 1e-033
Identities = 39/66 (59%), Positives = 45/66 (68%)
Frame = -3 / -3
Query: 665 EGASGCLPGSAASNAGCDMYSEWSLARVG*FDMSILVRSMAA*IWGARNTSASWTSSPVA 486
EGASG GSAA NAG MY+ + A+VG F +SI R MAA I GARNTS WTSSP+
Sbjct: 655 EGASG*FWGSAA*NAGISMYAS*NRAKVGYFVISIWKR*MAAWICGARNTSPKWTSSPME 476
Query: 485 YFPAHP 468
Y +HP
Sbjct: 475 YLLSHP 458
Score = 66.1 bits (138), Expect(4) = 3e-069
Identities = 26/31 (83%), Positives = 27/31 (87%)
Frame = +1 / +3
Query: 34 MLYSYWRSSCSHRARIALNLKGVDYEYKAVN 126
+LY W SSCSHR RIALNLKGVDYEYKAVN
Sbjct: 45 ILYGAWISSCSHRVRIALNLKGVDYEYKAVN 137
Score = 53.8 bits (111), Expect(3) = 1e-033
Identities = 30/61 (49%), Positives = 35/61 (57%)
Frame = -3 / -3
Query: 350 WRG*IPEATMLAIW*FKAFFXXXXXXXXXXGYLSSKYSVIA*ESPITELPSTNAGTNFIG 171
WRG + E TM AI +AFF GYLSS+Y +IA ES T+ STNAG IG
Sbjct: 349 WRGWMLEQTMFAI*RSRAFFFRSLGKRGC*GYLSSRYKMIASESDTTKSSSTNAGIYLIG 170
Query: 170 L 168
L
Sbjct: 169 L 167
Score = 43.2 bits (88), Expect(3) = 1e-033
Identities = 22/43 (51%), Positives = 27/43 (62%)
Frame = -3 / -3
Query: 134 LRRFTALYS*STPLRLRAMRARWEHELRQYEYSISRPTVASTS 6
+R TALYS*STPLRLRA+ R E EL Q Y + ++S
Sbjct: 145 VRGLTALYS*STPLRLRAILTR*EQELIQAPYRMGFAVAIASS 17
Score = 25.8 bits (50), Expect(4) = 3e-069
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +1 / +3
Query: 382 KVGAGESVLWTQQQIERGFTAIENLI 459
++ ES+ Q+ I++GF AIE L+
Sbjct: 378 RLSPDESLQLVQRYIDKGFRAIEKLL 455
>gb|CN817117.1|CN817117 HRO4528_D08_H16ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_D08_H16, mRNA sequence
Length = 802
Score = 114 bits (243), Expect(4) = 3e-069
Identities = 47/69 (68%), Positives = 52/69 (75%)
Frame = +1 / +2
Query: 463 LKGCAGKYATGDEVQLADVFLAPQIYAAIERTKIDMSNYPTLARLHSEYMSHPAFEAALP 642
L GC KY+ GDEV L DVFLAPQI+AAI R IDM+ YPTLAR H YM PAF+AALP
Sbjct: 437 LDGCDSKYSIGDEVHLGDVFLAPQIHAAIHRFHIDMTKYPTLARFHDAYMEIPAFQAALP 616
Query: 643 GKQPDAPSS 669
QPDAPS+
Sbjct: 617 QNQPDAPST 643
Score = 108 bits (230), Expect(4) = 3e-069
Identities = 44/75 (58%), Positives = 59/75 (78%)
Frame = +1 / +2
Query: 142 QSDPEFVKLNPMKFVPALVDGSSVIGDSYAITLYLEDKYPEPPLLPQDLQKKALNHQIAS 321
++D ++ K+NP+K++PALVD V+ DS AI LYLEDKYP+ PLLP+DL+KKAL+ QIA+
Sbjct: 125 RTDTDYEKINPIKYIPALVDEDFVVSDSLAIILYLEDKYPQHPLLPKDLKKKALDLQIAN 304
Query: 322 IVASGIQPLHNLTVL 366
IV S IQPL V+
Sbjct: 305 IVCSSIQPLQGYGVI 349
Score = 79.0 bits (166), Expect(3) = 1e-033
Identities = 39/66 (59%), Positives = 45/66 (68%)
Frame = -3 / -2
Query: 665 EGASGCLPGSAASNAGCDMYSEWSLARVG*FDMSILVRSMAA*IWGARNTSASWTSSPVA 486
EGASG GSAA NAG MY+ + A+VG F +SI R MAA I GARNTS WTSSP+
Sbjct: 639 EGASG*FWGSAA*NAGISMYAS*NRAKVGYFVISIWKR*MAAWICGARNTSPKWTSSPME 460
Query: 485 YFPAHP 468
Y +HP
Sbjct: 459 YLLSHP 442
Score = 66.1 bits (138), Expect(4) = 3e-069
Identities = 26/31 (83%), Positives = 27/31 (87%)
Frame = +1 / +2
Query: 34 MLYSYWRSSCSHRARIALNLKGVDYEYKAVN 126
+LY W SSCSHR RIALNLKGVDYEYKAVN
Sbjct: 29 ILYGAWISSCSHRVRIALNLKGVDYEYKAVN 121
Score = 53.8 bits (111), Expect(3) = 1e-033
Identities = 30/61 (49%), Positives = 35/61 (57%)
Frame = -3 / -2
Query: 350 WRG*IPEATMLAIW*FKAFFXXXXXXXXXXGYLSSKYSVIA*ESPITELPSTNAGTNFIG 171
WRG + E TM AI +AFF GYLSS+Y +IA ES T+ STNAG IG
Sbjct: 333 WRGWMLEQTMFAI*RSRAFFFRSLGKRGC*GYLSSRYKMIASESDTTKSSSTNAGIYLIG 154
Query: 170 L 168
L
Sbjct: 153 L 151
Score = 43.2 bits (88), Expect(3) = 1e-033
Identities = 22/43 (51%), Positives = 27/43 (62%)
Frame = -3 / -2
Query: 134 LRRFTALYS*STPLRLRAMRARWEHELRQYEYSISRPTVASTS 6
+R TALYS*STPLRLRA+ R E EL Q Y + ++S
Sbjct: 129 VRGLTALYS*STPLRLRAILTR*EQELIQAPYRMGFAVAIASS 1
Score = 25.8 bits (50), Expect(4) = 3e-069
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +1 / +2
Query: 382 KVGAGESVLWTQQQIERGFTAIENLI 459
++ ES+ Q+ I++GF AIE L+
Sbjct: 362 RLSPDESLQLVQRYIDKGFRAIEKLL 439
>gb|CN817427.1|CN817427 HRO4465_G11_N21ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4465_G11_N21, mRNA sequence
Length = 688
Score = 110 bits (235), Expect(2) = 1e-047
Identities = 46/71 (64%), Positives = 52/71 (73%)
Frame = +1 / +2
Query: 463 LKGCAGKYATGDEVQLADVFLAPQIYAAIERTKIDMSNYPTLARLHSEYMSHPAFEAALP 642
L+GC K A GDEVQ+ DVFLAPQI+A + R +IDMS YP LARL Y HPAF+AALP
Sbjct: 233 LEGCDSKCANGDEVQMGDVFLAPQIHAGVTRFQIDMSKYPILARLQQAYNEHPAFQAALP 412
Query: 643 GKQPDAPSSS* 675
QPDAP S *
Sbjct: 413 QNQPDAPPSQ* 445
Score = 95.0 bits (201), Expect(2) = 1e-047
Identities = 42/79 (53%), Positives = 56/79 (70%)
Frame = +1 / +2
Query: 226 YAITLYLEDKYPEPPLLPQDLQKKALNHQIASIVASGIQPLHNLTVLRFIDQKVGAGESV 405
+AI LY+ED+YP+ PLLPQDL+ KALN QIASIV S IQPL + V+ +GA ES+
Sbjct: 2 FAIILYMEDRYPQNPLLPQDLKNKALNLQIASIVCSSIQPLQSHAVIGSYLGTMGADESL 181
Query: 406 LWTQQQIERGFTAIENLIQ 462
+ I++GF AIE L++
Sbjct: 182 EMVRHYIDKGFKAIEKLLE 238
Score = 84.5 bits (178), Expect(2) = 5e-026
Identities = 39/67 (58%), Positives = 47/67 (70%)
Frame = -3 / -2
Query: 668 DEGASGCLPGSAASNAGCDMYSEWSLARVG*FDMSILVRSMAA*IWGARNTSASWTSSPV 489
D GASG GSAA NAGC + + SLA++G FD+SI R A IWGARNTS WTSSP+
Sbjct: 438 DGGASGWF*GSAA*NAGCSL*AC*SLAKIGYFDISIWKRVTPAWIWGARNTSPIWTSSPL 259
Query: 488 AYFPAHP 468
A+ +HP
Sbjct: 258 AHLLSHP 238
Score = 48.3 bits (99), Expect(2) = 5e-026
Identities = 33/75 (44%), Positives = 40/75 (53%)
Frame = -3 / -2
Query: 452 FSIAVKPLSICC*VHNTLSPAPTFWSMNLNTVRLWRG*IPEATMLAIW*FKAFFXXXXXX 273
FSIA+KPLSI C + LS AP T L RG + E TMLAI +A F
Sbjct: 228 FSIALKPLSI*CRTISRLSSAPIVPRYEPITAWL*RGWMLEHTMLAICKLRALFFRSWGK 49
Query: 272 XXXXGYLSSKYSVIA 228
GYLSS Y+++A
Sbjct: 48 RGF*GYLSSIYNMMA 4
>gb|CN818645.1|CN818645 HRO4467_E04_I07ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4467_E04_I07, mRNA sequence
Length = 478
Score = 133 bits (285), Expect = 4e-032
Identities = 53/73 (72%), Positives = 64/73 (87%)
Frame = +1 / +2
Query: 463 LKGCAGKYATGDEVQLADVFLAPQIYAAIERTKIDMSNYPTLARLHSEYMSHPAFEAALP 642
+K CAGKYATGDEVQLADVFLAPQI+AA+ R +IDMSNYPTL +++ EY HPAFEAALP
Sbjct: 23 IKDCAGKYATGDEVQLADVFLAPQIFAAVTRFQIDMSNYPTLGKVNDEYTRHPAFEAALP 202
Query: 643 GKQPDAPSSS*EL 681
+QPDAP ++*+L
Sbjct: 203 DRQPDAPPTA*KL 241
Score = 118 bits (253), Expect = 1e-027
Identities = 54/75 (72%), Positives = 57/75 (76%)
Frame = -3 / -2
Query: 689 LGCSS*EDEGASGCLPGSAASNAGCDMYSEWSLARVG*FDMSILVRSMAA*IWGARNTSA 510
L CS * GASGCL GSAASNAGC +YS + RVG*FD+SI R AA IWGARNTSA
Sbjct: 249 LSCSF*AVGGASGCLSGSAASNAGCLVYSSLTFPRVG*FDISI*KRVTAAKIWGARNTSA 70
Query: 509 SWTSSPVAYFPAHPF 465
SWTSSPVAYFPA F
Sbjct: 69 SWTSSPVAYFPAQSF 25
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,759,133
Number of Sequences: 8143
Number of extensions: 159542
Number of successful extensions: 1194
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 316507880
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)