TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3802264.2.1
(1189 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821037.1|CN821037 HRO4422_G12_M24ZS5 Lib AA069E1X Aven... 66 5e-033
gb|CN821305.1|CN821305 HRO4423_G12_N23ZS5 Lib AA069E1X Aven... 66 6e-032
gb|CN820388.1|CN820388 HRO4418_G08_M16ZS5 Lib AA069E1X Aven... 66 1e-027
gb|CN821108.1|CN821108 HRO4427_F02_L03ZS5 Lib AA069E1X Aven... 65 1e-026
gb|CN821143.1|CN821143 HRO4428_A02_B04ZS5 Lib AA069E1X Aven... 63 1e-023
>gb|CN821037.1|CN821037 HRO4422_G12_M24ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4422_G12_M24, mRNA
sequence
Length = 784
Score = 65.7 bits (137), Expect(3) = 5e-033
Identities = 29/64 (45%), Positives = 37/64 (57%)
Frame = +1 / +2
Query: 712 TDVNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGP 891
+DV G D++ PNPN GA+VGGP +ND FVD R N+ TE TY + +G
Sbjct: 344 SDVKTGCKGFDYLHSTSPNPNIAMGALVGGPFQNDSFVDLRDNAVQTESSTYNSGTLVGL 523
Query: 892 LAAL 903
LA L
Sbjct: 524 LAGL 535
Score = 65.7 bits (137), Expect(3) = 5e-033
Identities = 26/54 (48%), Positives = 33/54 (61%)
Frame = +1 / +2
Query: 535 VLCGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
V C + P + FA Q +Y+LG NP SY+VG+G+N P Q HHRGAS P
Sbjct: 182 VECSGKYFSPTDIRNFAISQANYILGDNPMKLSYLVGYGSNYPQQVHHRGASIP 343
Score = 43.2 bits (88), Expect(3) = 5e-033
Identities = 18/53 (33%), Positives = 32/53 (60%)
Frame = +1 / +2
Query: 361 SQADNFVCAVLPDTAFHQVFITPGGVIHLRDGANSQYVTSTAFLLVVYADLLL 519
++A+ +C +LPD+ T GG++ + + Q+ T+ AFL VVY+D +L
Sbjct: 5 TRAEAVICGLLPDSPSATGSRTSGGLVWISGWNSLQHATNAAFLAVVYSDYML 163
>gb|CN821305.1|CN821305 HRO4423_G12_N23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4423_G12_N23, mRNA
sequence
Length = 815
Score = 65.7 bits (137), Expect(3) = 6e-032
Identities = 29/64 (45%), Positives = 37/64 (57%)
Frame = +1 / +1
Query: 712 TDVNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGP 891
+DV G D++ PNPN GA+VGGP +ND FVD R N+ TE TY + +G
Sbjct: 319 SDVKTGCKGFDYLHSTSPNPNIAMGALVGGPFQNDSFVDLRDNAVQTESSTYNSGTLVGL 498
Query: 892 LAAL 903
LA L
Sbjct: 499 LAGL 510
Score = 65.7 bits (137), Expect(3) = 6e-032
Identities = 26/54 (48%), Positives = 33/54 (61%)
Frame = +1 / +1
Query: 535 VLCGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
V C + P + FA Q +Y+LG NP SY+VG+G+N P Q HHRGAS P
Sbjct: 157 VECSGKYFSPTYIRNFAISQANYILGDNPMKLSYLVGYGSNYPQQVHHRGASIP 318
Score = 39.6 bits (80), Expect(3) = 6e-032
Identities = 17/46 (36%), Positives = 27/46 (58%)
Frame = +1 / +1
Query: 382 CAVLPDTAFHQVFITPGGVIHLRDGANSQYVTSTAFLLVVYADLLL 519
C +LPD+ T GG++ + + Q+ T+ AFL VVY+D +L
Sbjct: 1 CGLLPDSPSATGSRTSGGLVWISGWNSLQHATNAAFLAVVYSDYML 138
>gb|CN820388.1|CN820388 HRO4418_G08_M16ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4418_G08_M16, mRNA
sequence
Length = 679
Score = 65.7 bits (137), Expect(3) = 1e-027
Identities = 29/64 (45%), Positives = 37/64 (57%)
Frame = +1 / +3
Query: 712 TDVNCGLSFGDWMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGP 891
+DV G D++ PNPN GA+VGGP +ND FVD R N+ TE TY + +G
Sbjct: 252 SDVKTGCKGFDYLHSTSPNPNIAMGALVGGPFQNDSFVDLRDNAVQTESSTYNSGTLVGL 431
Query: 892 LAAL 903
LA L
Sbjct: 432 LAGL 443
Score = 65.7 bits (137), Expect(3) = 1e-027
Identities = 26/54 (48%), Positives = 33/54 (61%)
Frame = +1 / +3
Query: 535 VLCGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
V C + P + FA Q +Y+LG NP SY+VG+G+N P Q HHRGAS P
Sbjct: 90 VECSGKYFSPTDIRNFAISQANYILGDNPMKLSYLVGYGSNYPQQVHHRGASIP 251
Score = 24.9 bits (48), Expect(3) = 1e-027
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +1 / +3
Query: 463 SQYVTSTAFLLVVYADLLL 519
S T+ AFL VVY+D +L
Sbjct: 15 SPVATNAAFLAVVYSDYML 71
>gb|CN821108.1|CN821108 HRO4427_F02_L03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_F02_L03, mRNA
sequence
Length = 714
Score = 65.2 bits (136), Expect(3) = 1e-026
Identities = 26/52 (50%), Positives = 33/52 (63%)
Frame = +1 / +2
Query: 541 CGNQPLPPARLHEFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
CG L +FA+ Q+DY+LG NP+ SYVVG+G P + HHRGAS P
Sbjct: 221 CGPNFYKTDVLRKFAKSQIDYILGKNPQKMSYVVGYGKKYPKRVHHRGASIP 376
Score = 62.5 bits (130), Expect(3) = 1e-026
Identities = 26/54 (48%), Positives = 33/54 (61%)
Frame = +1 / +2
Query: 745 WMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLAALA 906
W K NPN L GA+V GPD++DGF D R N +YTEP N+ + L +LA
Sbjct: 416 WRESKKANPNILVGAMVAGPDRHDGFKDIRTNYNYTEPTLAANAGLVAALISLA 577
Score = 25.4 bits (49), Expect(3) = 1e-026
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1 / +2
Query: 466 QYVTSTAFLLVVYADLL 516
QYV + AFL +YAD L
Sbjct: 143 QYVVNAAFLASLYADYL 193
>gb|CN821143.1|CN821143 HRO4428_A02_B04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4428_A02_B04, mRNA
sequence
Length = 636
Score = 62.9 bits (131), Expect(2) = 1e-023
Identities = 23/40 (57%), Positives = 30/40 (75%)
Frame = +1 / +2
Query: 577 EFARQQMDYLLGANPRHSSYVVGFGANPPTQPHHRGASTP 696
+FA+ Q+DY+LG NP+ SYVVG+G P + HHRGAS P
Sbjct: 14 KFAKSQIDYILGKNPQKMSYVVGYGKKYPKRVHHRGASIP 133
Score = 62.5 bits (130), Expect(2) = 1e-023
Identities = 26/54 (48%), Positives = 33/54 (61%)
Frame = +1 / +2
Query: 745 WMAPDKPNPNELTGAIVGGPDKNDGFVDKRGNSSYTEPCTYINSLAIGPLAALA 906
W K NPN L GA+V GPD++DGF D R N +YTEP N+ + L +LA
Sbjct: 173 WRESKKANPNILVGAMVAGPDRHDGFKDIRTNYNYTEPTLAANAGLVAALISLA 334
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,218,293
Number of Sequences: 8143
Number of extensions: 131724
Number of successful extensions: 976
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 424427445
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)