TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3748535.2.1
         (619 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CN814661.1|CN814661  HRO4501_B06_C11ZS5 Lib AA071E1X Aven...   226   6e-064

>gb|CN814661.1|CN814661 HRO4501_B06_C11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
           sativa cDNA clone HRO4501_B06_C11, mRNA sequence
          Length = 842

 Score =  226 bits (488), Expect(2) = 6e-064
 Identities = 93/120 (77%), Positives = 102/120 (85%)
 Frame = +2 / +2

                                                                       
Query: 260 GETSREAIKRHQHFLALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLNNNDYHIS 439
           GE+ RE+IKRH+HFLALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGLNNNDY I+
Sbjct: 158 GESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDYQIT 337

                                                                       
Query: 440 RGKELISSAGLSEQCCFLKGDFMDMPISDNTFDAAYAIQATCHAPDAQGVYSEVYRVLKP 619
           RGKEL   AGLS  C F+K DFM+MP SDNTFDA YAI+ATCHAPD  G Y E+YRVLKP
Sbjct: 338 RGKELNRLAGLSGTCDFVKADFMNMPFSDNTFDAVYAIEATCHAPDPVGCYKEIYRVLKP 517




 Score =  137 bits (293), Expect = 2e-033
 Identities = 67/120 (55%), Positives = 79/120 (65%)
 Frame = -2 / -3

                                                                       
Query: 618 GFSTR*TSL*TPCASGXXXXXXXXXXASKVLSDIGMSMKSPFKKQHCSLKPAEEMSSLPL 439
           GF+TR* SL* P  SG          ASKVLS+ G+ MKS   K    L PA  +SS PL
Sbjct: 516 GFNTR*ISL*QPTGSGA*HVASMA*TASKVLSEKGIFMKSALTKSQVPLSPARRLSSFPL 337

                                                                       
Query: 438 EMW*SLLFSPVIDVELNLAISIKGPPIPHPTSKTFIPFLSPNCSAKKCWCRLMASREVSP 259
            + *SLLF+PV +VELNLAIS+ GPPIP PTSKTFIP  + +  A+KC CRLM S + SP
Sbjct: 336 VI**SLLFNPVTEVELNLAISLNGPPIPQPTSKTFIPGFNSS*RARKCSCRLMLSLKDSP 157




 Score = 99.1 bits (210), Expect = 6e-022
 Identities = 54/115 (46%), Positives = 70/115 (60%)
 Frame = -1 / -2

                                                                       
Query: 619 RFQHTVDLTIDALCIWCMTCSLYCIGRIKGVIRYWHVHEVPLQEAALLAQTC*RNELLAP 440
           RF +TVDL I A  I CMTC L  I  IK VI   H+HEV L +     ++C   ELL+P
Sbjct: 517 RF*YTVDLLIAANRIRCMTCCLNGINSIKSVIGERHIHEVCLDKVTSSTKSCQTIELLSP 338

                                                                  
Query: 439 *DVVVIVVQSSYRC*AESGDFY*RSPNSTSHIQNLHTLLEPQL*CQKMLVPLNGF 275
             ++V+VVQS    *A+SG+  * S  ST+H+QNLH   + +L  QKMLV  + F
Sbjct: 337 SYLIVVVVQSGN*G*AKSGNLS*WSTYSTAHVQNLHPWFQLKLKGQKMLVSFDAF 173




 Score = 32.7 bits (65), Expect(2) = 6e-064
 Identities = 10/17 (58%), Positives = 16/17 (94%)
 Frame = +1 / +2

                            
Query: 208 HGGKQESRKSNYTDLIS 258
           +GGK+E+RK NYTD+++
Sbjct: 32  YGGKEEARKFNYTDMVN 82



  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 7,692,068
Number of Sequences: 8143
Number of extensions: 107952
Number of successful extensions: 1392
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 199753403
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)