TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3742446.2.1
(598 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|X78433.1|ASLBDG A.sativa L. mRNA for beta-D-glucosidase 98 3e-040
gb|AF082991.1|AF082991 Avena sativa beta-D-glucosidase beta... 95 2e-039
gb|CN818764.1|CN818764 HRO4468_D02_G04ZS5 Lib AA070E1X Aven... 110 4e-032
gb|CN820950.1|CN820950 HRO4421_G11_M21ZS5 Lib AA069E1X Aven... 111 1e-025
gb|CN819927.1|CN819927 HRO4407_H12_P23ZS5 Lib AA069E1X Aven... 98 1e-021
gb|CN821463.1|CN821463 HRO4425_F05_K09ZS5 Lib AA069E1X Aven... 98 1e-021
gb|CN821540.1|CN821540 HRO4426_E01_I02ZS5 Lib AA069E1X Aven... 98 1e-021
>emb|X78433.1|ASLBDG A.sativa L. mRNA for beta-D-glucosidase
Length = 1898
Score = 97.7 bits (207), Expect(2) = 3e-040
Identities = 37/90 (41%), Positives = 55/90 (61%)
Frame = +2 / +1
Query: 317 DKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYSMQEI 496
+K+ G+IG++LD + YEP+ ++ FH+GWFL+P+V G YP+SM+ +
Sbjct: 883 NKFYKGDDGQIGMVLDVMAYEPYGNNFLDQQAQERAIDFHIGWFLEPMVRGDYPFSMRSL 1062
Query: 497 AKDRLPLFSDEEARMAKGSIDYVGINHYTS 586
DRLP F+ E S D+VGIN+YTS
Sbjct: 1063 VGDRLPFFTKSEQEKLVSSYDFVGINYYTS 1152
Score = 82.2 bits (173), Expect(2) = 3e-040
Identities = 30/50 (60%), Positives = 35/50 (70%)
Frame = +2 / +1
Query: 65 WLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPG 214
+L +IV+ + DYA CF FGD+VKNWFTFNEP LGY GLHAPG
Sbjct: 619 FLDRRIVKDYTDYATVCFEHFGDKVKNWFTFNEPHSFCGLGYGTGLHAPG 768
>gb|AF082991.1|AF082991 Avena sativa beta-D-glucosidase beta subunit precursor (P60b) mRNA,
complete cds
Length = 1923
Score = 95.5 bits (202), Expect(2) = 2e-039
Identities = 36/93 (38%), Positives = 55/93 (59%)
Frame = +2 / +1
Query: 320 KYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYSMQEIA 499
K+ G+IG+++D + YEP+ ++ FH+GWFL+P+V G YP+SM+ +
Sbjct: 889 KFYKGDDGQIGMVMDVMAYEPYGNNFVDQQAQERSIDFHIGWFLEPMVRGDYPFSMRSLV 1068
Query: 500 KDRLPLFSDEEARMAKGSIDYVGINHYTSFLHE 598
DRLP F+ E S D+VGIN+YT+ E
Sbjct: 1069 GDRLPFFTKSEQEKLVSSYDFVGINYYTARFSE 1167
Score = 81.7 bits (172), Expect(2) = 2e-039
Identities = 30/53 (56%), Positives = 35/53 (66%)
Frame = +2 / +1
Query: 65 WLSPKIVEAFADYAEFCFHAFGDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
+L +IV+ + DYA CF FGD+VKNW TFNEP L Y GLHAPG CS
Sbjct: 625 FLDRRIVKDYTDYATVCFEHFGDKVKNWITFNEPHSFCGLAYGTGLHAPGLCS 783
>gb|CN818764.1|CN818764 HRO4468_D02_G04ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4468_D02_G04, mRNA sequence
Length = 718
Score = 110 bits (235), Expect(2) = 4e-032
Identities = 45/116 (38%), Positives = 60/116 (51%)
Frame = +2 / +2
Query: 239 GNSTTEPYXXXXXXXXXXXXXXRRYRDKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXX 418
GNST EPY R YR+KYQ QKG +GI + W P ++S
Sbjct: 128 GNSTVEPYIAAHNMILAHASATRLYREKYQAMQKGVVGINVYSFWTYPLTNSTADLAATK 307
Query: 419 XXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSDEEARMAKGSIDYVGINHYTS 586
F GW L+P+V G YP M+ RLP F++ ++ KG+ID++GINHY S
Sbjct: 308 RYQDFMFGWTLEPLVFGDYPQVMKTNVGSRLPSFTESQSEFVKGAIDFIGINHYYS 475
Score = 41.8 bits (85), Expect(2) = 4e-032
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = +2 / +2
Query: 128 GDRVKNWFTFNEPRCVAALGYDNGLHAPGRCS 223
G RV W T +EP YD+G+ APGRCS
Sbjct: 2 GTRVSYWTTLDEPNIGPIGSYDSGIFAPGRCS 97
>gb|CN820950.1|CN820950 HRO4421_G11_M21ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4421_G11_M21, mRNA
sequence
Length = 840
Score = 111 bits (236), Expect = 1e-025
Identities = 37/80 (46%), Positives = 56/80 (70%)
Frame = +2 / +3
Query: 347 IGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYSMQEIAKDRLPLFSD 526
+GI+LDF WYE ++S FH+GWF+DP+++G YP MQ++ K+RLP F+
Sbjct: 12 VGIVLDFNWYEALTNSTEDQAAAQRARDFHIGWFVDPLINGHYPQIMQDLVKERLPRFTP 191
Query: 527 EEARMAKGSIDYVGINHYTS 586
++A++ KGS DY+GIN YT+
Sbjct: 192 DQAKLVKGSADYIGINQYTA 251
>gb|CN819927.1|CN819927 HRO4407_H12_P23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_H12_P23, mRNA
sequence
Length = 832
Score = 97.7 bits (207), Expect = 1e-021
Identities = 37/90 (41%), Positives = 55/90 (61%)
Frame = +2 / +2
Query: 317 DKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYSMQEI 496
+K+ G+IG++LD + YEP+ ++ FH+GWFL+P+V G YP+SM+ +
Sbjct: 53 NKFYKGDDGQIGMVLDVMAYEPYGNNFLDQQAQERAIDFHIGWFLEPMVRGDYPFSMRSL 232
Query: 497 AKDRLPLFSDEEARMAKGSIDYVGINHYTS 586
DRLP F+ E S D+VGIN+YTS
Sbjct: 233 VGDRLPFFTKSEQEKLVSSYDFVGINYYTS 322
>gb|CN821463.1|CN821463 HRO4425_F05_K09ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4425_F05_K09, mRNA
sequence
Length = 812
Score = 97.7 bits (207), Expect = 1e-021
Identities = 37/90 (41%), Positives = 55/90 (61%)
Frame = +2 / +3
Query: 317 DKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYSMQEI 496
+K+ G+IG++LD + YEP+ ++ FH+GWFL+P+V G YP+SM+ +
Sbjct: 51 NKFYKGDDGQIGMVLDVMAYEPYGNNFLDQQAQERAIDFHIGWFLEPMVRGDYPFSMRSL 230
Query: 497 AKDRLPLFSDEEARMAKGSIDYVGINHYTS 586
DRLP F+ E S D+VGIN+YTS
Sbjct: 231 VGDRLPFFTKSEQEKLVSSYDFVGINYYTS 320
>gb|CN821540.1|CN821540 HRO4426_E01_I02ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4426_E01_I02, mRNA
sequence
Length = 612
Score = 97.7 bits (207), Expect = 1e-021
Identities = 37/90 (41%), Positives = 55/90 (61%)
Frame = +2 / +2
Query: 317 DKYQLHQKGKIGILLDFVWYEPFSDSNXXXXXXXXXXXFHLGWFLDPIVHGRYPYSMQEI 496
+K+ G+IG++LD + YEP+ ++ FH+GWFL+P+V G YP+SM+ +
Sbjct: 50 NKFYKGDDGQIGMVLDVMAYEPYGNNFLDQQAQERAIDFHIGWFLEPMVRGDYPFSMRSL 229
Query: 497 AKDRLPLFSDEEARMAKGSIDYVGINHYTS 586
DRLP F+ E S D+VGIN+YTS
Sbjct: 230 VGDRLPFFTKSEQEKLVSSYDFVGINYYTS 319
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,956,467
Number of Sequences: 8143
Number of extensions: 122905
Number of successful extensions: 2106
Number of sequences better than 1.0e-020: 7
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 191175036
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)