TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3713013.2.1
(3807 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Aven... 217 1e-056
gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Aven... 193 2e-049
gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Aven... 182 3e-046
gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Aven... 116 2e-026
>gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C09_F17, mRNA
sequence
Length = 676
Score = 217 bits (468), Expect = 1e-056
Identities = 87/95 (91%), Positives = 90/95 (94%)
Frame = -2 / +1
Query: 527 VGVVAGISYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRQNRTPTIVVVWAILL 348
VGVVAG SYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGR NRTPTIV+VWAILL
Sbjct: 55 VGVVAGTSYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILL 234
Query: 347 ASIFSLLWVRIDPFTTRVTGPDTQTCGINC*GSGR 243
ASIFSLLWVRIDPFTTRV GP+ QTCGINC* +GR
Sbjct: 235 ASIFSLLWVRIDPFTTRVAGPNIQTCGINC*ENGR 339
Score = 199 bits (429), Expect = 3e-051
Identities = 82/110 (74%), Positives = 96/110 (87%)
Frame = +1 / -3
Query: 241 NLPLP*QLMPHVWVSGPVTRVVKGSMRTHSKEKMDASRMAQTTTMVGVRFCLPMRPLRNG 420
NLP *QL+P VW+ GP TRVV GS+RTHSKEK++A R+AQT T+VGVRF LP+RPLRNG
Sbjct: 341 NLPFS*QLIPQVWMFGPATRVVNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNG 162
Query: 421 YRWTMTQKAKKSLPKSGPHDWYPLLMA*EMPATTPTRLMIRMVVGGIRSV 570
Y+ T+TQKAKKS P SGPHDW PLLMA*E+PATTPT LMI++VVGG+RSV
Sbjct: 161 YK*TITQKAKKSFPNSGPHDW*PLLMA*EVPATTPTMLMIKIVVGGMRSV 12
>gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_H12_O23, mRNA
sequence
Length = 620
Score = 193 bits (416), Expect = 2e-049
Identities = 76/83 (91%), Positives = 79/83 (95%)
Frame = -2 / +2
Query: 491 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRQNRTPTIVVVWAILLASIFSLLWVRID 312
SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGR NRTPTIV+VWAILLASIFSLLWVRID
Sbjct: 2 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILLASIFSLLWVRID 181
Query: 311 PFTTRVTGPDTQTCGINC*GSGR 243
PFTTRV GP+ QTCGINC* +GR
Sbjct: 182 PFTTRVAGPNIQTCGINC*ENGR 250
Score = 155 bits (332), Expect = 6e-038
Identities = 62/84 (73%), Positives = 72/84 (85%)
Frame = +1 / -3
Query: 241 NLPLP*QLMPHVWVSGPVTRVVKGSMRTHSKEKMDASRMAQTTTMVGVRFCLPMRPLRNG 420
NLP *QL+P VW+ GP TRVV GS+RTHSKEK++A R+AQT T+VGVRF LP+RPLRNG
Sbjct: 252 NLPFS*QLIPQVWMFGPATRVVNGSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNG 73
Query: 421 YRWTMTQKAKKSLPKSGPHDWYPL 492
Y+ T+TQKAKKS PKSGPHDW PL
Sbjct: 72 YK*TITQKAKKSFPKSGPHDW*PL 1
>gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4510_G06_M12, mRNA sequence
Length = 521
Score = 182 bits (392), Expect = 3e-046
Identities = 69/91 (75%), Positives = 81/91 (89%)
Frame = -2 / +2
Query: 527 VGVVAGISYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRQNRTPTIVVVWAILL 348
VG+VAG+S A+N+GY SWGPLFGKLFF+FWVIVHLYPFLKGLMGRQNRTPTIVV+W++LL
Sbjct: 62 VGIVAGVSDAVNNGYGSWGPLFGKLFFSFWVIVHLYPFLKGLMGRQNRTPTIVVLWSVLL 241
Query: 347 ASIFSLLWVRIDPFTTRVTGPDTQTCGINC* 255
ASIFSL+WVRIDPF + GP + CG+ C*
Sbjct: 242 ASIFSLVWVRIDPFIAKSKGPILKPCGVQC* 334
Score = 156 bits (335), Expect = 2e-038
Identities = 68/103 (66%), Positives = 76/103 (73%)
Frame = +1 / -3
Query: 268 PHVWVSGPVTRVVKGSMRTHSKEKMDASRMAQTTTMVGVRFCLPMRPLRNGYRWTMTQKA 447
PH GP+ +KGS+ T ++EKM+ASR Q+TTMVGVRFCLPM PLRNG RWTMTQK
Sbjct: 321 PHGLRMGPLDLAMKGSILTQTREKMEASRTDQSTTMVGVRFCLPMSPLRNG*RWTMTQKE 142
Query: 448 KKSLPKSGPHDWYPLLMA*EMPATTPTRLMIRMVVGGIRSVVH 576
KKS PK GPHD PLL A E PAT PT LM+ VVGGI VVH
Sbjct: 141 KKSFPKRGPHDP*PLLTASETPATMPTMLMMMSVVGGISRVVH 13
>gb|CN815647.1|CN815647 HRO4514_A01_A02ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4514_A01_A02, mRNA sequence
Length = 474
Score = 116 bits (248), Expect = 2e-026
Identities = 43/60 (71%), Positives = 52/60 (86%)
Frame = -2 / +3
Query: 437 VIVHLYPFLKGLMGRQNRTPTIVVVWAILLASIFSLLWVRIDPFTTRVTGPDTQTCGINC 258
VIVHLYPFLKGL+GRQNRTPTIV+VW+ILLASI SLLWVR++PF + GP + CG++C
Sbjct: 12 VIVHLYPFLKGLLGRQNRTPTIVIVWSILLASIISLLWVRVNPFLAKTDGPTLEECGLDC 191
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 42,844,737
Number of Sequences: 8143
Number of extensions: 677230
Number of successful extensions: 4161
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 47
effective length of database: 1,184,766
effective search space used: 1446599286
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)