TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3713002.2.1
(923 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|Z15131.1|ASBGLUCAN A.sativa mRNA for beta glucanase 341 7e-095
gb|AY277385.1| Avena sativa 1,3-beta glucanase (Oglc13) gen... 122 5e-040
gb|CN821029.1|CN821029 HRO4422_G03_M06ZS5 Lib AA069E1X Aven... 158 1e-039
gb|AF155932.1| Avena sativa 1,3-beta glucanase (Oglc13) mRN... 120 2e-039
>emb|Z15131.1|ASBGLUCAN A.sativa mRNA for beta glucanase
Length = 1448
Score = 341 bits (740), Expect = 7e-095
Identities = 134/148 (90%), Positives = 142/148 (95%)
Frame = +3 / +1
Query: 3 RTGSPLMANIYPYLAWAYNPSAMDMSYALFTSSGTVVQDGAYGYQNLFDTTVDAFYVAMG 182
RTGSPLMANIYPYLAWAYNPSAMDMSYALFT+SGTVVQDGAYGYQNLFDTTVDAFY AMG
Sbjct: 589 RTGSPLMANIYPYLAWAYNPSAMDMSYALFTASGTVVQDGAYGYQNLFDTTVDAFYTAMG 768
Query: 183 KNGGSGVPLVVSESGWPSGGGVQATPANARVYNQYLINHVGRGTPRHPGAIETYLFSMFN 362
K+GG+GV LVVSESGWPS GG ATPANAR+YNQYLINHVGRGTPRHPG IETY+F+MFN
Sbjct: 769 KHGGAGVKLVVSESGWPSAGGEAATPANARIYNQYLINHVGRGTPRHPGGIETYVFAMFN 948
Query: 363 ENQKESGVEQNWGLFYPNMQHVYPISF* 446
ENQK++GVEQNWGLFYPNMQHVYPISF*
Sbjct: 949 ENQKDNGVEQNWGLFYPNMQHVYPISF* 1032
Score = 287 bits (622), Expect = 1e-078
Identities = 126/148 (85%), Positives = 129/148 (87%)
Frame = -2 / -1
Query: 445 QKLMG*TCCMLG*KSPQFCSTPLSFWFSLNMEKR*VSMAPGWRGVPRPTWLMRYWLYTLA 266
QKLMG*TCCMLG*KSPQFCSTPLSFWFSLNM *VSM PG RGVPRPTWL+RYWL LA
Sbjct: 1031 QKLMG*TCCMLG*KSPQFCSTPLSFWFSLNMANT*VSMPPGCRGVPRPTWLIRYWL*ILA 852
Query: 265 LAGVAWTPPPDGHPLSDTTSGTPEPPFFPMAT*KASTVVSKRFWYP*APSCTTVPEEVKS 86
LAGVA +PP DGHPLSDTTS TP PP P+A *KASTVVS RFWYP*APS TTVPE VKS
Sbjct: 851 LAGVAASPPADGHPLSDTTSFTPAPPCLPIAV*KASTVVSNRFWYP*APSWTTVPEAVKS 672
Query: 85 A*LMSMALGLYAQARYG*MLAMSGDPVR 2
A*LMSMALGL AQARYG*MLAMSG PVR
Sbjct: 671 A*LMSMALGL*AQARYG*MLAMSGLPVR 588
Score = 76.2 bits (160), Expect(3) = 2e-023
Identities = 31/35 (88%), Positives = 33/35 (94%)
Frame = +2 / +3
Query: 341 LPLLHVQREPEGERRGAELGALLPQHAARLPHQLL 445
L + HVQREPEG+RRGAELGALLPQHAARLPHQLL
Sbjct: 927 LRVRHVQREPEGQRRGAELGALLPQHAARLPHQLL 1031
Score = 74.8 bits (157), Expect(3) = 7e-030
Identities = 31/35 (88%), Positives = 31/35 (88%)
Frame = -1 / -3
Query: 107 GAGGGEERVAHVHGAGVVCPGQVRVDVGHERRPGA 3
GAGGGEERVAHVHGA VV PGQVRVDVGHER GA
Sbjct: 693 GAGGGEERVAHVHGARVVGPGQVRVDVGHERAAGA 589
Score = 56.1 bits (116), Expect(3) = 7e-030
Identities = 24/38 (63%), Positives = 27/38 (71%)
Frame = -1 / -3
Query: 290 EVLVVHPRVGRRGLDAAAGRPPALGHHERHAGAAVLSH 177
+VLVV P VGR AA RPP LGHH+ H GAAVL+H
Sbjct: 876 QVLVVDPGVGRGRSLAAGRRPPTLGHHQLHPGAAVLAH 763
Score = 49.6 bits (102), Expect(3) = 2e-023
Identities = 17/21 (80%), Positives = 19/21 (90%)
Frame = +2 / +3
Query: 2 PHRVAAHGQHLPVPGLGIQPQ 64
PHR AHGQHLPVPGLG+QP+
Sbjct: 588 PHRQPAHGQHLPVPGLGLQPE 650
Score = 32.7 bits (65), Expect(3) = 7e-030
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = -1 / -3
Query: 446 SEADGVDVLHVGV 408
SEADGVDVLHVGV
Sbjct: 1032 SEADGVDVLHVGV 994
Score = 21.7 bits (41), Expect(3) = 2e-023
Identities = 7/13 (53%), Positives = 7/13 (53%)
Frame = +1 / +2
Query: 178 WERTAAPACRSWC 216
W TAAP WC
Sbjct: 764 WASTAAPG*SWWC 802
>gb|AY277385.1| Avena sativa 1,3-beta glucanase (Oglc13) gene, complete cds
Length = 3281
Score = 122 bits (261), Expect(3) = 5e-040
Identities = 50/81 (61%), Positives = 60/81 (74%)
Frame = +3 / +2
Query: 132 YQNLFDTTVDAFYVAMGKNGGSGVPLVVSESGWPSGGGVQATPANARVYNQYLINHVGRG 311
Y NLFD VDA + A+ K G GV +VVSESGWP GG A+ NAR YNQ LI+HVG G
Sbjct: 2846 YTNLFDAMVDAVHAALEKAGAPGVKVVVSESGWPKTGGTGASTDNARAYNQGLIDHVGGG 3025
Query: 312 TPRHPGAIETYLFSMFNENQK 374
TP+ GA+ETY+F+MF+ENQK
Sbjct: 3026 TPKKRGALETYVFAMFDENQK 3088
Score = 99.1 bits (210), Expect(2) = 2e-024
Identities = 44/81 (54%), Positives = 51/81 (62%)
Frame = -2 / -3
Query: 373 FWFSLNMEKR*VSMAPGWRGVPRPTWLMRYWLYTLALAGVAWTPPPDGHPLSDTTSGTPE 194
FWFS N+ VS AP + GVP PTW + WLY LAL+ +A PP GHPLSDTT+ TP
Sbjct: 3087 FWFSSNIANTYVSSAPRFLGVPPPTWSISPWLYALALSVLAPVPPVLGHPLSDTTTFTPG 2908
Query: 193 PPFFPMAT*KASTVVSKRFWY 131
P F A AST+ S RF Y
Sbjct: 2907 APAFSSAACTASTIASNRFVY 2845
Score = 46.0 bits (94), Expect(3) = 5e-040
Identities = 17/35 (48%), Positives = 24/35 (68%)
Frame = +3 / +2
Query: 6 TGSPLMANIYPYLAWAYNPSAMDMSYALFTSSGTV 110
TG+PL+ANIYPY ++ NP + ++YA F TV
Sbjct: 2717 TGAPLLANIYPYFSYRDNPRDIQLNYATFRPGTTV 2821
Score = 29.5 bits (58), Expect(3) = 5e-040
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +3 / +2
Query: 378 SGVEQNWGLFYPNMQHVYPISF* 446
+G E+++GLF P+ YPI F*
Sbjct: 3095 AGTEKHFGLFNPDKSPAYPIRF* 3163
Score = 28.6 bits (56), Expect(2) = 2e-024
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = -3 / -1
Query: 60 GCMPRPGTGRCWP*AATRC 4
GC GRCWP AA C
Sbjct: 2771 GCRGTRSRGRCWPAAARPC 2715
>gb|CN821029.1|CN821029 HRO4422_G03_M06ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4422_G03_M06, mRNA
sequence
Length = 508
Score = 158 bits (340), Expect = 1e-039
Identities = 62/91 (68%), Positives = 72/91 (79%)
Frame = +3 / +3
Query: 102 GTVVQDGAYGYQNLFDTTVDAFYVAMGKNGGSGVPLVVSESGWPSGGGVQATPANARVYN 281
GTVVQDG+ YQNLFD VD FY A+G G V +VVSESGWPS GG A+ ANA+ YN
Sbjct: 9 GTVVQDGSNAYQNLFDALVDTFYSALGSAGAGNVKIVVSESGWPSAGGTAASAANAQTYN 188
Query: 282 QYLINHVGRGTPRHPGAIETYLFSMFNENQK 374
Q LINHVG+GTP+ PGAIETY+F+MFNEN+K
Sbjct: 189 QNLINHVGKGTPKRPGAIETYIFAMFNENKK 281
Score = 125 bits (268), Expect = 9e-030
Identities = 57/91 (62%), Positives = 60/91 (65%)
Frame = -2 / -1
Query: 373 FWFSLNMEKR*VSMAPGWRGVPRPTWLMRYWLYTLALAGVAWTPPPDGHPLSDTTSGTPE 194
F FSLNM VSMAPG GVP PTWLMR+WL ALA +A PP +GHPLSDTT T
Sbjct: 280 FLFSLNMANMYVSMAPGLLGVPFPTWLMRFWL*VCALAALAAVPPAEGHPLSDTTILTFP 101
Query: 193 PPFFPMAT*KASTVVSKRFWYP*APSCTTVP 101
P P A *K ST SKRFW PSCTTVP
Sbjct: 100 APALPKAE*KVSTRASKRFW*ALLPSCTTVP 8
>gb|AF155932.1| Avena sativa 1,3-beta glucanase (Oglc13) mRNA, partial cds
Length = 1045
Score = 120 bits (256), Expect(3) = 2e-039
Identities = 49/81 (60%), Positives = 60/81 (74%)
Frame = +3 / +1
Query: 132 YQNLFDTTVDAFYVAMGKNGGSGVPLVVSESGWPSGGGVQATPANARVYNQYLINHVGRG 311
Y NLFD VDA + A+ K G + V +VVSESGWP GG A+ NAR YNQ LI+HVG G
Sbjct: 601 YTNLFDAMVDAVHAALEKAGAASVKVVVSESGWPKEGGTGASVDNARAYNQGLIDHVGGG 780
Query: 312 TPRHPGAIETYLFSMFNENQK 374
TP+ GA+ETY+F+MF+ENQK
Sbjct: 781 TPKKRGALETYVFAMFDENQK 843
Score = 96.4 bits (204), Expect(2) = 1e-023
Identities = 43/79 (54%), Positives = 49/79 (62%)
Frame = -2 / -3
Query: 373 FWFSLNMEKR*VSMAPGWRGVPRPTWLMRYWLYTLALAGVAWTPPPDGHPLSDTTSGTPE 194
FWFS NM VS AP + GVP PTW + WLY LAL+ +A PP GHPLSDTT+ T
Sbjct: 842 FWFSSNMANTYVSSAPRFLGVPPPTWSISPWLYALALSTLAPVPPSFGHPLSDTTTFTLA 663
Query: 193 PPFFPMAT*KASTVVSKRF 137
P F A AST+ S RF
Sbjct: 662 APAFSSAACTASTIASNRF 606
Score = 46.0 bits (94), Expect(3) = 2e-039
Identities = 17/35 (48%), Positives = 24/35 (68%)
Frame = +3 / +1
Query: 6 TGSPLMANIYPYLAWAYNPSAMDMSYALFTSSGTV 110
TG+PL+ANIYPY ++ NP + ++YA F TV
Sbjct: 472 TGAPLLANIYPYFSYRDNPRDIQLNYATFRPGTTV 576
Score = 29.5 bits (58), Expect(3) = 2e-039
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +3 / +1
Query: 378 SGVEQNWGLFYPNMQHVYPISF* 446
+G E+++GLF P+ YPI F*
Sbjct: 850 AGTEKHFGLFNPDKSPAYPIRF* 918
Score = 28.6 bits (56), Expect(2) = 1e-023
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = -3 / -1
Query: 60 GCMPRPGTGRCWP*AATRC 4
GC GRCWP AA C
Sbjct: 526 GCRGTRSRGRCWPAAARPC 470
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,613,458
Number of Sequences: 8143
Number of extensions: 110123
Number of successful extensions: 968
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 321377232
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)