TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3588987.2.1
(487 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815408.1|CN815408 HRO4509_C11_E21ZS5 Lib AA071E1X Aven... 192 4e-050
gb|CN815415.1|CN815415 HRO4509_D07_G13ZS5 Lib AA071E1X Aven... 192 4e-050
gb|CN816540.1|CN816540 HRO4521_H08_O15ZS5 Lib AA071E1X Aven... 192 4e-050
gb|CN819629.1|CN819629 HRO4402_D05_G10ZS5 Lib AA069E1X Aven... 192 4e-050
>gb|CN815408.1|CN815408 HRO4509_C11_E21ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_C11_E21, mRNA sequence
Length = 894
Score = 192 bits (413), Expect = 4e-050
Identities = 77/104 (74%), Positives = 94/104 (90%)
Frame = -1 / +2
Query: 487 LETYQAGLKHXPKNQELLDGVRRCVEQITKASRGELSEEELKERQNKAMQDPEIQNILTD 308
+ETYQAGLKH P NQELLDG+RRC+EQI KA+RG++S+E+L+E+Q+KAMQDPEIQ ILTD
Sbjct: 452 METYQAGLKHDPNNQELLDGIRRCIEQINKANRGDISQEDLQEKQSKAMQDPEIQGILTD 631
Query: 307 PIMQQVLTDLQENPRAAQAHLKNPGVMQKIQKLVSAGIVQMK*T 176
PIM+QVL D QENP AAQ HLK+PGV QKIQKL++AGIVQ +*+
Sbjct: 632 PIMRQVLMDFQENPSAAQHHLKDPGVAQKIQKLINAGIVQTR*S 763
Score = 102 bits (218), Expect = 3e-023
Identities = 52/102 (50%), Positives = 64/102 (62%)
Frame = +2 / -3
Query: 179 LLHLNYPCADELLDLLHHSGVLEVRLRSPRVLLQIRQNLLHDRISQDVLDLRVLHGFVLS 358
L L+Y ELL+LL ++ VL++ L S RVLL+I Q L HDR+ +D LD VLH F L
Sbjct: 760 LSRLDYSSVYELLNLLCNTRVLKMMLSSTRVLLEIHQYLSHDRVGKDSLDFWVLHSFTLF 581
Query: 359 LFQFLLTEFASARLGDLFNTPPYTIK*LLVFRXML*ASLISF 484
L LLT L +L NTPPYTIK LV R +L SL+SF
Sbjct: 580 LL*VLLTYITPVSLVNLLNTPPYTIKQFLVIRIVLQPSLVSF 455
Score = 74.4 bits (156), Expect(2) = 3e-024
Identities = 33/52 (63%), Positives = 37/52 (71%)
Frame = +3 / -1
Query: 180 YFI*TIPALTSFWIFCITPGFLRCACAARGFSCRSVKTCCMIGSVRMFWISG 335
Y + TIPA SF IFC TPG LR AA GFS +S+ TC MIGSVR+ WISG
Sbjct: 759 YLVWTIPAFMSF*IFCATPGSLR*C*AALGFSWKSINTCRMIGSVRIPWISG 604
Score = 51.5 bits (106), Expect(2) = 3e-024
Identities = 20/28 (71%), Positives = 22/28 (78%)
Frame = +3 / -1
Query: 402 VICSTHLLTPSSNSWFFGXCFRPA**VS 485
+ICS HLL PSS+SW FG CF PA *VS
Sbjct: 537 LICSIHLLIPSSSSWLFGSCFNPAW*VS 454
>gb|CN815415.1|CN815415 HRO4509_D07_G13ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_D07_G13, mRNA sequence
Length = 725
Score = 192 bits (413), Expect = 4e-050
Identities = 77/104 (74%), Positives = 94/104 (90%)
Frame = -1 / -1
Query: 487 LETYQAGLKHXPKNQELLDGVRRCVEQITKASRGELSEEELKERQNKAMQDPEIQNILTD 308
+ETYQAGLKH P NQELLDG+RRC+EQI KA+RG++S+E+L+E+Q+KAMQDPEIQ ILTD
Sbjct: 503 METYQAGLKHDPNNQELLDGIRRCIEQINKANRGDISQEDLQEKQSKAMQDPEIQGILTD 324
Query: 307 PIMQQVLTDLQENPRAAQAHLKNPGVMQKIQKLVSAGIVQMK*T 176
PIM+QVL D QENP AAQ HLK+PGV QKIQKL++AGIVQ +*+
Sbjct: 323 PIMRQVLMDFQENPSAAQHHLKDPGVAQKIQKLINAGIVQTR*S 192
Score = 102 bits (218), Expect = 3e-023
Identities = 52/102 (50%), Positives = 64/102 (62%)
Frame = +2 / +3
Query: 179 LLHLNYPCADELLDLLHHSGVLEVRLRSPRVLLQIRQNLLHDRISQDVLDLRVLHGFVLS 358
L L+Y ELL+LL ++ VL++ L S RVLL+I Q L HDR+ +D LD VLH F L
Sbjct: 195 LSRLDYSSVYELLNLLCNTRVLKMMLSSTRVLLEIHQYLSHDRVGKDSLDFWVLHSFTLF 374
Query: 359 LFQFLLTEFASARLGDLFNTPPYTIK*LLVFRXML*ASLISF 484
L LLT L +L NTPPYTIK LV R +L SL+SF
Sbjct: 375 LL*VLLTYITPVSLVNLLNTPPYTIKQFLVIRIVLQPSLVSF 500
Score = 74.4 bits (156), Expect(2) = 3e-024
Identities = 33/52 (63%), Positives = 37/52 (71%)
Frame = +3 / +1
Query: 180 YFI*TIPALTSFWIFCITPGFLRCACAARGFSCRSVKTCCMIGSVRMFWISG 335
Y + TIPA SF IFC TPG LR AA GFS +S+ TC MIGSVR+ WISG
Sbjct: 196 YLVWTIPAFMSF*IFCATPGSLR*C*AALGFSWKSINTCRMIGSVRIPWISG 351
Score = 51.5 bits (106), Expect(2) = 3e-024
Identities = 20/28 (71%), Positives = 22/28 (78%)
Frame = +3 / +1
Query: 402 VICSTHLLTPSSNSWFFGXCFRPA**VS 485
+ICS HLL PSS+SW FG CF PA *VS
Sbjct: 418 LICSIHLLIPSSSSWLFGSCFNPAW*VS 501
>gb|CN816540.1|CN816540 HRO4521_H08_O15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4521_H08_O15, mRNA sequence
Length = 771
Score = 192 bits (413), Expect = 4e-050
Identities = 77/104 (74%), Positives = 94/104 (90%)
Frame = -1 / +2
Query: 487 LETYQAGLKHXPKNQELLDGVRRCVEQITKASRGELSEEELKERQNKAMQDPEIQNILTD 308
+ETYQAGLKH P NQELLDG+RRC+EQI KA+RG++S+E+L+E+Q+KAMQDPEIQ ILTD
Sbjct: 296 METYQAGLKHDPNNQELLDGIRRCIEQINKANRGDISQEDLQEKQSKAMQDPEIQGILTD 475
Query: 307 PIMQQVLTDLQENPRAAQAHLKNPGVMQKIQKLVSAGIVQMK*T 176
PIM+QVL D QENP AAQ HLK+PGV QKIQKL++AGIVQ +*+
Sbjct: 476 PIMRQVLMDFQENPSAAQHHLKDPGVAQKIQKLINAGIVQTR*S 607
Score = 101 bits (215), Expect = 9e-023
Identities = 51/102 (50%), Positives = 63/102 (61%)
Frame = +2 / -3
Query: 179 LLHLNYPCADELLDLLHHSGVLEVRLRSPRVLLQIRQNLLHDRISQDVLDLRVLHGFVLS 358
L L+Y ELL+LL ++ +L++ L RVLL+I Q L HDRI +D LD VLH F L
Sbjct: 604 LSRLDYSSVYELLNLLCNTRILKMMLSCTRVLLEIHQYLSHDRIGKDALDFWVLHSFTLF 425
Query: 359 LFQFLLTEFASARLGDLFNTPPYTIK*LLVFRXML*ASLISF 484
L LLT L +L NTPPYTIK LV R +L SL+SF
Sbjct: 424 LL*VLLTYITPVSLVNLLNTPPYTIKQFLVIRIVLQPSLVSF 299
Score = 76.2 bits (160), Expect(2) = 8e-025
Identities = 34/52 (65%), Positives = 37/52 (71%)
Frame = +3 / -1
Query: 180 YFI*TIPALTSFWIFCITPGFLRCACAARGFSCRSVKTCCMIGSVRMFWISG 335
Y + TIPA SF IFC TPG LR AA GFS +S+ TC MIGSVRM WISG
Sbjct: 603 YLVWTIPAFMSF*IFCATPGSLR*C*AALGFSWKSINTCRMIGSVRMPWISG 448
Score = 51.5 bits (106), Expect(2) = 8e-025
Identities = 20/28 (71%), Positives = 22/28 (78%)
Frame = +3 / -1
Query: 402 VICSTHLLTPSSNSWFFGXCFRPA**VS 485
+ICS HLL PSS+SW FG CF PA *VS
Sbjct: 381 LICSIHLLIPSSSSWLFGSCFNPAW*VS 298
>gb|CN819629.1|CN819629 HRO4402_D05_G10ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4402_D05_G10, mRNA
sequence
Length = 766
Score = 192 bits (413), Expect = 4e-050
Identities = 77/104 (74%), Positives = 94/104 (90%)
Frame = -1 / +3
Query: 487 LETYQAGLKHXPKNQELLDGVRRCVEQITKASRGELSEEELKERQNKAMQDPEIQNILTD 308
+ETYQAGLKH P NQELLDG+RRC+EQI KA+RG++S+E+L+E+Q+KAMQDPEIQ ILTD
Sbjct: 288 METYQAGLKHDPNNQELLDGIRRCIEQINKANRGDISQEDLQEKQSKAMQDPEIQGILTD 467
Query: 307 PIMQQVLTDLQENPRAAQAHLKNPGVMQKIQKLVSAGIVQMK*T 176
PIM+QVL D QENP AAQ HLK+PGV QKIQKL++AGIVQ +*+
Sbjct: 468 PIMRQVLMDFQENPSAAQHHLKDPGVAQKIQKLINAGIVQTR*S 599
Score = 102 bits (218), Expect = 3e-023
Identities = 52/102 (50%), Positives = 64/102 (62%)
Frame = +2 / -3
Query: 179 LLHLNYPCADELLDLLHHSGVLEVRLRSPRVLLQIRQNLLHDRISQDVLDLRVLHGFVLS 358
L L+Y ELL+LL ++ VL++ L S RVLL+I Q L HDR+ +D LD VLH F L
Sbjct: 596 LSRLDYSSVYELLNLLCNTRVLKMMLSSTRVLLEIHQYLSHDRVGKDSLDFWVLHSFTLF 417
Query: 359 LFQFLLTEFASARLGDLFNTPPYTIK*LLVFRXML*ASLISF 484
L LLT L +L NTPPYTIK LV R +L SL+SF
Sbjct: 416 LL*VLLTYITPVSLVNLLNTPPYTIKQFLVIRIVLQPSLVSF 291
Score = 74.4 bits (156), Expect(2) = 3e-024
Identities = 33/52 (63%), Positives = 37/52 (71%)
Frame = +3 / -1
Query: 180 YFI*TIPALTSFWIFCITPGFLRCACAARGFSCRSVKTCCMIGSVRMFWISG 335
Y + TIPA SF IFC TPG LR AA GFS +S+ TC MIGSVR+ WISG
Sbjct: 595 YLVWTIPAFMSF*IFCATPGSLR*C*AALGFSWKSINTCRMIGSVRIPWISG 440
Score = 51.5 bits (106), Expect(2) = 3e-024
Identities = 20/28 (71%), Positives = 22/28 (78%)
Frame = +3 / -1
Query: 402 VICSTHLLTPSSNSWFFGXCFRPA**VS 485
+ICS HLL PSS+SW FG CF PA *VS
Sbjct: 373 LICSIHLLIPSSSSWLFGSCFNPAW*VS 290
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,199,325
Number of Sequences: 8143
Number of extensions: 66911
Number of successful extensions: 1956
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 41
effective length of database: 1,233,624
effective search space used: 148034880
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)