TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3276099.2.1
(545 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816950.1|CN816950 HRO4526_F04_K08ZS5 Lib AA071E1X Aven... 185 6e-048
>gb|CN816950.1|CN816950 HRO4526_F04_K08ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4526_F04_K08, mRNA sequence
Length = 701
Score = 185 bits (398), Expect = 6e-048
Identities = 78/88 (88%), Positives = 83/88 (94%)
Frame = +3 / +2
Query: 27 IDRSHFQALVHFYGCKLQVQERMTRQIAEAVYSVSHNGAMVVVEANHICMISRGIEKIRS 206
IDRSHFQALVHFYGCKLQVQERMTRQIAEAVYSVSH GA+VVVEANHICMISRGIEKIRS
Sbjct: 2 IDRSHFQALVHFYGCKLQVQERMTRQIAEAVYSVSHRGAIVVVEANHICMISRGIEKIRS 181
Query: 207 NTATIAVLGQFLADPSAKACFLQNVLDS 290
+TATIAVLG FL D SAKA FLQN++D+
Sbjct: 182 STATIAVLGHFLTDSSAKASFLQNIIDT 265
Score = 159 bits (342), Expect(2) = 2e-041
Identities = 73/94 (77%), Positives = 80/94 (85%)
Frame = -2 / -3
Query: 307 HTAEPTESKTFCKKHALAEGSAKNWPKTAIVAVLLLIFSIPLEIMQMWLASTTTMAPLCE 128
+++ S FC+K ALAE S + WPKTAIVAVLLLIFSIPL+IMQMW ASTTTMAP CE
Sbjct: 282 YSSRSAVSIMFCRKDALAEESVRKWPKTAIVAVLLLIFSIPLDIMQMWFASTTTMAPRCE 103
Query: 127 TE*TASAICLVILS*T*SLHP*K*TNA*KCDRSM 26
TE*TASAICLVILS*T*SLHP*K*T A*K +RSM
Sbjct: 102 TE*TASAICLVILS*T*SLHP*K*TRA*KWERSM 1
Score = 133 bits (286), Expect = 2e-032
Identities = 55/88 (62%), Positives = 64/88 (72%)
Frame = +2 / +1
Query: 26 H*SVAFSGVGSFLRVQASGSRKDDKADS*SGLFGFAQWGHGRC*GQPHLHDLKGDRENKE 205
H*S+ FSG GSFLRVQASGS KDD+ DS*S LF F W H C G+PHLHD+K DREN+E
Sbjct: 1 H*SLPFSGSGSFLRVQASGSGKDDETDS*SSLFRFTPWSHSCCRGEPHLHDIKRDRENQE 180
Query: 206 QHGNNCSFGPVFGRPFCQGVLLTECFGF 289
+ NNCSFGP FCQG+ TE + +
Sbjct: 181 *YSNNCSFGPFSD*FFCQGIFSTEHYRY 264
Score = 131 bits (281), Expect = 8e-032
Identities = 58/87 (66%), Positives = 65/87 (74%)
Frame = -1 / -2
Query: 287 IQNIL*EARLGRRVGQKLAQNCNCCRVAPYFLYPP*DHANVVGLNNDHGPIVRNRINRFS 108
I N+L*+ LGRR+ QK+AQNCNCC P FLYP * HA+VV L N++G V N IN FS
Sbjct: 262 IYNVL*KRCLGRRISQKMAQNCNCCCTTPDFLYPS*YHADVVRLYNNYGSTV*NGINCFS 83
Query: 107 YLPCHPFLNLKLAPVEMNQRLKMRPIN 27
YL HPFLNLKLAPVEMNQ LKM IN
Sbjct: 82 YLSRHPFLNLKLAPVEMNQSLKMGAIN 2
Score = 110 bits (234), Expect = 2e-025
Identities = 56/90 (62%), Positives = 62/90 (68%)
Frame = +1 / +3
Query: 28 LIGRIFRRWFISTGASFRFKKG*QGR*LKRFIRFRTMGPWSLLRPTTFA*SQGG*RK*GA 207
LI IFR WFISTGASFRF+KG*+ R*LK+FI F T+ P L R TT A* Q G*RK G
Sbjct: 3 LIAPIFRLWFISTGASFRFRKG*RDR*LKQFIPFHTVEP*LL*RRTTSA*YQEG*RKSGV 182
Query: 208 TRQQLQFWASFWPTLLPRRASYRMFWILLV 297
+QQLQFWA F LLPR YR I L+
Sbjct: 183 VQQQLQFWAIF*LILLPRHLFYRTL*IPLI 272
Score = 25.8 bits (50), Expect(2) = 2e-041
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = -2 / -1
Query: 499 QVTWYNTREPKPFWCFLVTSS 437
Q+ WYNT K F+ L+ +S
Sbjct: 518 QIKWYNTGGTKTFFLVLLVTS 456
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,096,820
Number of Sequences: 8143
Number of extensions: 102710
Number of successful extensions: 1903
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 170341859
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)