TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3204398.2.1
(666 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818277.1|CN818277 HRO4470_C12_F24ZS5 Lib AA070E1X Aven... 193 2e-050
gb|CN820072.1|CN820072 HRO4410_G07_M14ZS5 Lib AA069E1X Aven... 193 2e-050
gb|CN816332.1|CN816332 HRO4519_E08_J15ZS5 Lib AA071E1X Aven... 158 7e-040
gb|CN820119.1|CN820119 HRO4411_C12_F23ZS5 Lib AA069E1X Aven... 111 1e-025
>gb|CN818277.1|CN818277 HRO4470_C12_F24ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4470_C12_F24, mRNA sequence
Length = 620
Score = 193 bits (416), Expect = 2e-050
Identities = 80/89 (89%), Positives = 86/89 (96%)
Frame = +1 / +1
Query: 1 TSGNFEFALLAILRCAENPAKYFAKLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEY 180
TSGNFE AL+ ILRCAENPAKYFAK+LRK+MKGLGTDDKTL+RVVVTRTEIDMQYIKAEY
Sbjct: 214 TSGNFEVALVTILRCAENPAKYFAKVLRKSMKGLGTDDKTLVRVVVTRTEIDMQYIKAEY 393
Query: 181 FKKYKKPLAEAIHSETSGNYRTFLLSLVG 267
+KKYKKPLA+AIHSETSG YRTFLLSLVG
Sbjct: 394 YKKYKKPLADAIHSETSGGYRTFLLSLVG 480
Score = 166 bits (357), Expect = 3e-042
Identities = 73/88 (82%), Positives = 76/88 (86%)
Frame = -2 / -1
Query: 266 PTSERRNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISVLSSVPRPFMA 87
PTSE RNVR* PDVSEWIASA+GFLYFL YSA +YCISISVLVTTTL SVLSSVPRPFM
Sbjct: 479 PTSESRNVR*PPDVSEWIASASGFLYFL*YSAFMYCISISVLVTTTLTSVLSSVPRPFMD 300
Query: 86 FRKSFAKYFAGFSAHLRIAKSANSKFPD 3
F + AKYFAGFSAHLRI SA SKFPD
Sbjct: 299 FLNTLAKYFAGFSAHLRIVTSATSKFPD 216
Score = 105 bits (223), Expect = 1e-023
Identities = 48/88 (54%), Positives = 64/88 (72%)
Frame = +3 / +3
Query: 3 IWKL*IRTFSYPQMRGKPSKVFCKALTKGHERSRH**QDTYKGCGDED*N*YAIYQGRVL 182
IWK * T +YPQM + S+VFC + + HERS +* QDT KG G+++ +*YAI++GRVL
Sbjct: 216 IWKF*SCTGNYPQMC*ESSEVFC*GVKEIHERSWY*RQDTCKGGGNQNRD*YAIHKGRVL 395
Query: 183 QEV*EAIS*GYPLRNIRKLSDIPSFTCW 266
QE+ +A S* YP RNI LSD+P+ CW
Sbjct: 396 QEIQKAAS*CYPFRNIGGLSDVPALACW 479
>gb|CN820072.1|CN820072 HRO4410_G07_M14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4410_G07_M14, mRNA
sequence
Length = 798
Score = 193 bits (416), Expect = 2e-050
Identities = 80/89 (89%), Positives = 86/89 (96%)
Frame = +1 / +2
Query: 1 TSGNFEFALLAILRCAENPAKYFAKLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEY 180
TSGNFE AL+ ILRCAENPAKYFAK+LRK+MKGLGTDDKTL+RVVVTRTEIDMQYIKAEY
Sbjct: 320 TSGNFEVALVTILRCAENPAKYFAKVLRKSMKGLGTDDKTLVRVVVTRTEIDMQYIKAEY 499
Query: 181 FKKYKKPLAEAIHSETSGNYRTFLLSLVG 267
+KKYKKPLA+AIHSETSG YRTFLLSLVG
Sbjct: 500 YKKYKKPLADAIHSETSGGYRTFLLSLVG 586
Score = 166 bits (357), Expect = 3e-042
Identities = 73/88 (82%), Positives = 76/88 (86%)
Frame = -2 / -1
Query: 266 PTSERRNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISVLSSVPRPFMA 87
PTSE RNVR* PDVSEWIASA+GFLYFL YSA +YCISISVLVTTTL SVLSSVPRPFM
Sbjct: 585 PTSESRNVR*PPDVSEWIASASGFLYFL*YSAFMYCISISVLVTTTLTSVLSSVPRPFMD 406
Query: 86 FRKSFAKYFAGFSAHLRIAKSANSKFPD 3
F + AKYFAGFSAHLRI SA SKFPD
Sbjct: 405 FLNTLAKYFAGFSAHLRIVTSATSKFPD 322
Score = 105 bits (223), Expect = 1e-023
Identities = 48/88 (54%), Positives = 64/88 (72%)
Frame = +3 / +1
Query: 3 IWKL*IRTFSYPQMRGKPSKVFCKALTKGHERSRH**QDTYKGCGDED*N*YAIYQGRVL 182
IWK * T +YPQM + S+VFC + + HERS +* QDT KG G+++ +*YAI++GRVL
Sbjct: 322 IWKF*SCTGNYPQMC*ESSEVFC*GVKEIHERSWY*RQDTCKGGGNQNRD*YAIHKGRVL 501
Query: 183 QEV*EAIS*GYPLRNIRKLSDIPSFTCW 266
QE+ +A S* YP RNI LSD+P+ CW
Sbjct: 502 QEIQKAAS*CYPFRNIGGLSDVPALACW 585
>gb|CN816332.1|CN816332 HRO4519_E08_J15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4519_E08_J15, mRNA sequence
Length = 546
Score = 158 bits (340), Expect = 7e-040
Identities = 66/71 (92%), Positives = 70/71 (98%)
Frame = +1 / +3
Query: 55 PAKYFAKLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEYFKKYKKPLAEAIHSETSG 234
PAKYFAK+LRK+MKGLGTDDKTLIRVVVTRTEIDMQYIKAEY+KKYKKPLA+AIHSETSG
Sbjct: 12 PAKYFAKVLRKSMKGLGTDDKTLIRVVVTRTEIDMQYIKAEYYKKYKKPLADAIHSETSG 191
Query: 235 NYRTFLLSLVG 267
YRTFLLSLVG
Sbjct: 192 GYRTFLLSLVG 224
Score = 134 bits (287), Expect = 2e-032
Identities = 59/71 (83%), Positives = 62/71 (87%)
Frame = -2 / -3
Query: 266 PTSERRNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISVLSSVPRPFMA 87
PTSE RNVR* PDVSEWIASA+GFLYFL YSA +YCISISVLVTTTLI VLSSVPRPFM
Sbjct: 223 PTSESRNVR*PPDVSEWIASASGFLYFL*YSAFMYCISISVLVTTTLIRVLSSVPRPFMD 44
Query: 86 FRKSFAKYFAG 54
F + AKYFAG
Sbjct: 43 FLNTLAKYFAG 11
Score = 95.5 bits (202), Expect = 8e-021
Identities = 40/72 (55%), Positives = 57/72 (79%)
Frame = +3 / +2
Query: 51 KPSKVFCKALTKGHERSRH**QDTYKGCGDED*N*YAIYQGRVLQEV*EAIS*GYPLRNI 230
+ S+VFC + + HERS +**QD+YKG G+++ +*YAI++GRVLQE+ +A S* YP RNI
Sbjct: 8 RTSEVFC*GVKEIHERSWY**QDSYKGGGNQNRD*YAIHKGRVLQEIQKAAS*CYPFRNI 187
Query: 231 RKLSDIPSFTCW 266
+LSD+P+ CW
Sbjct: 188 GRLSDVPALACW 223
>gb|CN820119.1|CN820119 HRO4411_C12_F23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4411_C12_F23, mRNA
sequence
Length = 480
Score = 111 bits (237), Expect = 1e-025
Identities = 46/50 (92%), Positives = 49/50 (98%)
Frame = +1 / +1
Query: 118 TLIRVVVTRTEIDMQYIKAEYFKKYKKPLAEAIHSETSGNYRTFLLSLVG 267
TL+RVVVTRTEIDMQYIKAEY+KKYKKPLA+AIHSETSG YRTFLLSLVG
Sbjct: 13 TLVRVVVTRTEIDMQYIKAEYYKKYKKPLADAIHSETSGGYRTFLLSLVG 162
Score = 97.3 bits (206), Expect = 2e-021
Identities = 43/50 (86%), Positives = 45/50 (90%)
Frame = -2 / -2
Query: 266 PTSERRNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISV 117
PTSE RNVR* PDVSEWIASA+GFLYFL YSA +YCISISVLVTTTL SV
Sbjct: 161 PTSESRNVR*PPDVSEWIASASGFLYFL*YSAFMYCISISVLVTTTLTSV 12
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,732,649
Number of Sequences: 8143
Number of extensions: 110039
Number of successful extensions: 1118
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 219361099
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)