TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3204272.2.1
(739 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815151.1|CN815151 HRO4506_D10_G20ZS5 Lib AA071E1X Aven... 188 7e-049
gb|CN818949.1|CN818949 HRO4474_G09_N18ZS5 Lib AA070E1X Aven... 174 2e-044
>gb|CN815151.1|CN815151 HRO4506_D10_G20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4506_D10_G20, mRNA sequence
Length = 599
Score = 188 bits (406), Expect = 7e-049
Identities = 84/104 (80%), Positives = 92/104 (88%)
Frame = -3 / +1
Query: 500 ITWQDSENSFLRVKVINFGSDAVTLTISTSGLQASINALGSTATVLTSADVMDENSFSNP 321
ITWQDS NSFLRVK++NFGSD V+LTIS +GLQASIN LGS ATVLTS++VMDENSFSNP
Sbjct: 25 ITWQDSGNSFLRVKIVNFGSDQVSLTISATGLQASINTLGSNATVLTSSNVMDENSFSNP 204
Query: 320 TKVVPVKSDLSNAAEQMQVTLAPRSFSSFDLALAQSKLVAEM*A 189
KVVPV S L NAAEQMQVTL SFSSFDLALAQS+LVAEM*+
Sbjct: 205 NKVVPVTSQLRNAAEQMQVTLPAHSFSSFDLALAQSQLVAEM*S 336
Score = 179 bits (385), Expect = 5e-046
Identities = 85/114 (74%), Positives = 89/114 (78%)
Frame = +1 / -1
Query: 178 CTSLAHISATSLD*ARARSNELKERGASVTCICSAALLRSLFTGTTLVGLLKEFSSITSA 357
C S HISATS D*A ARSNE KE SVTCICSAAL L TGTTL GLLKEFSSIT
Sbjct: 347 CLSPLHISATSCD*ASARSNEEKE*AGSVTCICSAALRSWLVTGTTLFGLLKEFSSITLL 168
Query: 358 DVRTVAVDPRALILAWSPLVEIVRVTASDPKFMTFTLRKLFSESCQVIADAASE 519
DVRTVA DPR L+LA SP+ EIVR+T S+PKF FTLRKLF ESCQVIADAA E
Sbjct: 167 DVRTVAFDPRVLMLA*SPVAEIVRLTWSEPKFTIFTLRKLFPESCQVIADAARE 6
Score = 133 bits (284), Expect = 4e-032
Identities = 58/111 (52%), Positives = 77/111 (69%)
Frame = +3 / -3
Query: 189 SSHLRYKLGLSQGEIERAEGARSQRDLHLLCSIAQIALYRHNLGWVAEGVFIHHIC*CEN 368
+SHLR KL LSQ ++ER EG + DLHLL S+AQ+A +RHNL WV EG+FIHHI CEN
Sbjct: 336 ASHLRDKL*LSQRKVERGEGVSRECDLHLLRSVAQLACHRHNLVWVTEGIFIHHIAGCEN 157
Query: 369 SGG*SQSVDTGLEPTCGDCQGHSI*PEVYDLYS*EAVLGVLPGNRRRCK*T 521
S SVD L+P DC+ H + +V++L+S EA+ +LPG+ R C+ T
Sbjct: 156 SSVRP*SVDASLKPGRRDCEAHLVRTKVHNLHSQEAIPRILPGDSRCCQRT 4
Score = 117 bits (251), Expect = 2e-027
Identities = 58/119 (48%), Positives = 73/119 (61%)
Frame = -2 / +3
Query: 519 FTCSVCDYLAGLREQLPKSKGHKLRVRCCDPDNLHKWAPGQYQRSGINRHCSHISRCDG* 340
F+ S+C +LAG E LP+S+ +L +P NL A G +Q S + R+CSHI +CDG*
Sbjct: 6 FSGSICYHLAGFWE*LPESEDCELWFGPGEPHNLCDRASG*HQHSRVERYCSHIQQCDG* 185
Query: 339 KLLQQPNQGCAGKERSEQCCRADAGHAGSSLLQLVRSRPGSVQACSGDVS*AGTLVYNT 163
K LQ P QGCA + + Q C ADAGH SLL LVR GSV AC GDV T + +T
Sbjct: 186 KFLQ*PKQGCACDKPAAQRCGADAGHTPCSLLLLVRPCAGSVTACRGDVKRRQTEIRDT 362
Score = 106 bits (226), Expect = 4e-024
Identities = 61/107 (57%), Positives = 66/107 (61%)
Frame = -1 / +2
Query: 511 QRLRLPGRTPRTAS*E*RS*TSGQML*P*QSPQVGSRPVSTLWDQPPLFSHQQM*WMKTP 332
Q L PGR AS*E*R *T + * QS + G R STL + LFSH M*WMK P
Sbjct: 14 QHLLSPGRILGIAS*E*RL*TLVRTR*ASQSLRPGFRLASTL*GRTLLFSHPAM*WMKIP 193
Query: 331 SATQPRLCR*RAI*AMLQSRCRSRWLLAPSARSISPWLSPSL*RRCE 191
S TQ RLC *+A A L+SRCRS LL PS RS WLS SL RRCE
Sbjct: 194 SVTQTRLCL*QASCATLRSRCRSHSLLTPSPRSTLRWLSHSLSRRCE 334
>gb|CN818949.1|CN818949 HRO4474_G09_N18ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4474_G09_N18, mRNA sequence
Length = 495
Score = 174 bits (374), Expect = 2e-044
Identities = 78/97 (80%), Positives = 87/97 (89%)
Frame = -3 / +3
Query: 479 NSFLRVKVINFGSDAVTLTISTSGLQASINALGSTATVLTSADVMDENSFSNPTKVVPVK 300
NSFLRVK++NFGSD V+LTI+ +GLQASINALGS ATVLTS++VMDENSFSNP KVVPV
Sbjct: 12 NSFLRVKIVNFGSDQVSLTITATGLQASINALGSNATVLTSSNVMDENSFSNPNKVVPVT 191
Query: 299 SDLSNAAEQMQVTLAPRSFSSFDLALAQSKLVAEM*A 189
S L NAAEQMQVTL SFSSFDLALAQS+LVAEM*+
Sbjct: 192 SQLRNAAEQMQVTLPAHSFSSFDLALAQSQLVAEM*S 302
Score = 149 bits (319), Expect = 7e-037
Identities = 72/99 (72%), Positives = 77/99 (77%)
Frame = +1 / -3
Query: 184 SLAHISATSLD*ARARSNELKERGASVTCICSAALLRSLFTGTTLVGLLKEFSSITSADV 363
SL HISATS D*A ARSNE KE SVTCICSAAL L TGTTL GLLKEFSSIT DV
Sbjct: 307 SLLHISATSCD*ASARSNEEKE*AGSVTCICSAALRSWLVTGTTLFGLLKEFSSITLLDV 128
Query: 364 RTVAVDPRALILAWSPLVEIVRVTASDPKFMTFTLRKLF 480
RTVA +P AL+LA SP+ IVR+T S+PKF FTLRKLF
Sbjct: 127 RTVAFEPSALMLA*SPVAVIVRLTWSEPKFTIFTLRKLF 11
Score = 110 bits (235), Expect = 3e-025
Identities = 49/97 (50%), Positives = 67/97 (69%)
Frame = +3 / -2
Query: 189 SSHLRYKLGLSQGEIERAEGARSQRDLHLLCSIAQIALYRHNLGWVAEGVFIHHIC*CEN 368
+SHLR KL LSQ ++ER EG + DLHLL S+AQ+A +RHNL V EG+FIHHI +N
Sbjct: 302 ASHLRDKL*LSQRKVERGEGVSRECDLHLLRSVAQLACHRHNLVRVTEGIFIHHIAGRQN 123
Query: 369 SGG*SQSVDTGLEPTCGDCQGHSI*PEVYDLYS*EAV 479
S +Q VD L+P DC+ H + +V++L+S EA+
Sbjct: 122 SSVRTQRVDASLKPGRSDCEAHLVRTKVHNLHSQEAI 12
Score = 103 bits (219), Expect = 4e-023
Identities = 50/99 (50%), Positives = 61/99 (61%)
Frame = -2 / +2
Query: 489 GLREQLPKSKGHKLRVRCCDPDNLHKWAPGQYQRSGINRHCSHISRCDG*KLLQQPNQGC 310
G RE LP+S+ +L +P N A G +QR+G R+CS + +CDG*K LQ P QGC
Sbjct: 2 GTRE*LPESEDCELWFGPGEPHNHCDRASG*HQRAGFERYCSDVQQCDG*KFLQ*PEQGC 181
Query: 309 AGKERSEQCCRADAGHAGSSLLQLVRSRPGSVQACSGDV 193
A + + Q C ADAGH SLL LVR GSV AC GDV
Sbjct: 182 ACDKPAAQRCGADAGHTPCSLLLLVRPCAGSVTACRGDV 298
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,119,137
Number of Sequences: 8143
Number of extensions: 141093
Number of successful extensions: 1233
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 245902276
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)